Restriction map for
pFN21AA2021
(Circular) (Six-base) MAPSORT of: pFN21AA2021.seq Check: 5825 from: 1 to: 6613
pFN21AA2021 6613 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4423 278
Size: 53 83 186 936 2887 2468
Fragments arranged by size:
2887 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2592 3956 1937
Size: 655 1364 4594
Fragments arranged by size:
4594 1364 655
Acc65I G'GTAC_C
Cuts at: 3934 3934
Size: 6613
AclI AA'CG_TT
Cuts at: 1563 4740 5113 1563
Size: 3177 373 3063
Fragments arranged by size:
3177 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2621 2721 2759 2819 4676 6060 6328 1632
Size: 989 100 38 60 1857 1384 268 1917
Fragments arranged by size:
1917 1857 1384 989 268 100 60 38
AfeI AGC'GCT
Cuts at: 3116 3116
Size: 6613
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6416
AflIII A'CryG_T
Cuts at: 1203 5512 6489 1203
Size: 4309 977 1327
Fragments arranged by size:
4309 1327 977
AgeI A'CCGG_T
Cuts at: 5444 5444
Size: 6613
AhdI GACnn_n'nnGTC
Cuts at: 5342 5342
Size: 6613
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6613
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2345 3710 3882 1184
Size: 1161 1365 172 3915
Fragments arranged by size:
3915 1365 1161 172
AlwNI CAG_nnn'CTG
Cuts at: 2341 2782 2841 3010 3079 3401 3481 3547 3775
Size: 441 59 169 69 322 80 66 228
Cuts at: 3775 4356 5928 2341
Size: 581 1572 3026
Fragments arranged by size:
3026 1572 581 441 322 228 169 80
69 66 59
ApaI G_GGCC'C
Cuts at: 3733 3733
Size: 6613
ApaLI G'TGCA_C
Cuts at: 2261 4670 5826 2261
Size: 2409 1156 3048
Fragments arranged by size:
3048 2409 1156
ApoI r'AATT_y
Cuts at: 1493 3922 4148 4300 6409 1493
Size: 2429 226 152 2109 1697
Fragments arranged by size:
2429 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5167 160
Size: 872 4135 1606
Fragments arranged by size:
4135 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6613
AvaI C'yCGr_G
Cuts at: 1870 1943 2231 3938 1870
Size: 73 288 1707 4545
Fragments arranged by size:
4545 1707 288 73
BamHI G'GATC_C
Cuts at: 3943 4310 3943
Size: 367 6246
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2040 2091 3524 3934 5389
Size: 325 217 627 253 51 1433 410 1455
Cuts at: 5389 618
Size: 1842
Fragments arranged by size:
1842 1455 1433 627 410 325 253 217
51
BanII G_rGCy'C
Cuts at: 1391 2180 2789 3027 3176 3309 3733 3932 1391
Size: 789 609 238 149 133 424 199 4072
Fragments arranged by size:
4072 789 609 424 238 199 149 133
BbeI G_GCGC'C
Cuts at: 2044 2095 2044
Size: 51 6562
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2717 2837 3915 928
Size: 971 818 120 1078 3626
Fragments arranged by size:
3626 1078 971 818 120
BbvCI CC'TCA_GC
Cuts at: 2568 2568
Size: 6613
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4804 4838 1903
Size: 34 2867 34 3678
Fragments arranged by size:
3678 2867 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4505 5715 4505
Size: 1210 5403
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6613
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3728 5224 136
Size: 107 122 71 631 605 2056 1496 1525
Fragments arranged by size:
2056 1525 1496 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2251 2829 6608 2251
Size: 578 3779 2256
Fragments arranged by size:
3779 2256 578
BlpI GC'TnA_GC
Cuts at: 3170 3750 4030 3170
Size: 580 280 5753
Fragments arranged by size:
5753 580 280
Bme1580I G_kGCm'C
Cuts at: 1790 2265 3733 4674 5830 1790
Size: 475 1468 941 1156 2573
Fragments arranged by size:
2573 1468 1156 941 475
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6613
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2247 2878 4344
Size: 416 53 83 399 24 830 631 1466
Cuts at: 4344 5302 442
Size: 958 1753
Fragments arranged by size:
1753 1466 958 830 631 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6613
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2139 2561 2681 2818 3020 3458 3730 5273
Size: 900 422 120 137 202 438 272 1543
Cuts at: 5273 1239
Size: 2579
Fragments arranged by size:
2579 1543 900 438 422 272 202 137
120
Bpu10I CC'TnA_GC
Cuts at: 2174 2568 2721 2783 3141 2174
Size: 394 153 62 358 5646
Fragments arranged by size:
5646 394 358 153 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3745 4738 5603 5901 3745
Size: 993 865 298 4457
Fragments arranged by size:
4457 993 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2528 3468 3777 5276 882
Size: 617 1029 940 309 1499 2219
Fragments arranged by size:
2219 1499 1029 940 617 309
BsaAI yAC'GTr
Cuts at: 493 1204 4404 493
Size: 711 3200 2702
Fragments arranged by size:
3200 2702 711
BsaBI GATnn'nnATC
Cuts at: 6607 6607
Size: 6613
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2041 2092 4420 4802
Size: 53 83 186 936 508 51 2328 382
Cuts at: 4802 5437 275
Size: 635 1451
Fragments arranged by size:
2328 1451 936 635 508 382 186 83
53 51
BsaWI w'CCGG_w
Cuts at: 2131 5046 5444 5718 5865 6276 2131
Size: 2915 398 274 147 411 2468
Fragments arranged by size:
2915 2468 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2771 2801 1178
Size: 30 165 30 1368 30 4990
Fragments arranged by size:
4990 1368 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2504 2507 2510 2513 2516 2519 2522 2558
Size: 1318 3 3 3 3 3 3 36
Cuts at: 2558 2779 3065 3179 3398 3401 1186
Size: 221 286 114 219 3 4398
Fragments arranged by size:
4398 1318 286 221 219 114 36 3
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2050 2473 2716 5816 6312 2050
Size: 423 243 3100 496 2351
Fragments arranged by size:
3100 2351 496 423 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3069 3321 3470 1163
Size: 573 1333 252 149 4306
Fragments arranged by size:
4306 1333 573 252 149
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4063 4824 5852 1540
Size: 275 186 2062 761 1028 2301
Fragments arranged by size:
2301 2062 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2265 2789 3057 3411 3585 4674 4759 5830 2265
Size: 524 268 354 174 1089 85 1071 3048
Fragments arranged by size:
3048 1089 1071 524 354 268 174 85
BsmI GAATG_Cn'
Cuts at: 3290 4124 4217 3290
Size: 834 93 5686
Fragments arranged by size:
5686 834 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2180 2265 2789 3027 3057 3176 3309
Size: 399 390 85 524 238 30 119 133
Cuts at: 3309 3411 3585 3733 3932 4674 4759 5830 1391
Size: 102 174 148 199 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 524 399 390 238 199
174 148 133 119 102 85 85 30
BspEI T'CCGG_A
Cuts at: 2131 6276 2131
Size: 4145 2468
BspHI T'CATG_A
Cuts at: 4502 6232 4502
Size: 1730 4883
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2995 3968 844
Size: 2151 973 3489
Fragments arranged by size:
3489 2151 973
BsrBI CCG'CTC
Cuts at: 1394 1823 4500 1394
Size: 429 2677 3507
Fragments arranged by size:
3507 2677 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2805 2911 3093 3219 3426 5108 5282
Size: 1183 1557 106 182 126 207 1682 174
Cuts at: 5282 65
Size: 1396
Fragments arranged by size:
1682 1557 1396 1183 207 182 174 126
106
BsrFI r'CCGG_y
Cuts at: 1700 1810 2043 2149 5257 5444 1700
Size: 110 233 106 3108 187 2869
Fragments arranged by size:
3108 2869 233 187 110 106
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6613
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6613
BssSI C'ACGA_G
Cuts at: 4673 5685 4673
Size: 1012 5601
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3010 3401 3484 794
Size: 2216 391 83 3923
Fragments arranged by size:
3923 2216 391 83
BstBI TT'CG_AA
Cuts at: 3514 5417 3514
Size: 1903 4710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6613
BstXI CCAn_nnnn'nTGG
Cuts at: 2998 3352 3439 2998
Size: 354 87 6172
Fragments arranged by size:
6172 354 87
BstYI r'GATC_y
Cuts at: 1972 2251 2829 3943 4310 4695 4712 6164 6250
Size: 279 578 1114 367 385 17 1452 86
Cuts at: 6250 6262 6608 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1114 578 385 367 346 279
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2529 2683 3682 513
Size: 552 939 525 154 999 3444
Fragments arranged by size:
3444 999 939 552 525 154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5874
Fragments arranged by size:
5874 683 56
BtsI GCAGTG_nn'
Cuts at: 2329 2817 3402 4129 4923 4943 2329
Size: 488 585 727 794 20 3999
Fragments arranged by size:
3999 794 727 585 488 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6613
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6578
DraI TTT'AAA
Cuts at: 3917 3917
Size: 6613
DraIII CAC_nnn'GTG
Cuts at: 2035 3415 3802 2035
Size: 1380 387 4846
Fragments arranged by size:
4846 1380 387
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5620 809
Size: 507 4304 1802
Fragments arranged by size:
4304 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2123 3423 3793
Size: 54 1425 50 275 18 293 1300 370
Cuts at: 3793 3873 4060 4949 6578 8
Size: 80 187 889 1629 43
Fragments arranged by size:
1629 1425 1300 889 370 293 275 187
80 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4060 1537
Size: 275 2248 4090
Fragments arranged by size:
4090 2248 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4543 1353
Size: 3190 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2189 5200 5574 5720 387
Size: 1064 138 600 3011 374 146 1280
Fragments arranged by size:
3011 1280 1064 600 374 146 138
EcoICRI GAG'CTC
Cuts at: 2787 2787
Size: 6613
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2139 2561 2621 2681 2721 2759 2818
Size: 393 507 422 60 60 40 38 59
Cuts at: 2818 2819 3020 3458 3730 4676 5273 6060 6328
Size: 1 201 438 272 946 597 787 268
Cuts at: 6328 1239
Size: 1524
Fragments arranged by size:
1524 946 787 597 507 438 422 393
272 268 201 60 60 59 40 38
1
EcoNI CCTnn'n_nnAGG
Cuts at: 3002 3047 3002
Size: 45 6568
EcoO109I rG'GnC_Cy
Cuts at: 2293 2679 2779 3007 3234 3252 3268 3729 2293
Size: 386 100 228 227 18 16 461 5177
Fragments arranged by size:
5177 461 386 228 227 100 18 16
EcoRI G'AATT_C
Cuts at: 3922 4300 3922
Size: 378 6235
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3804 3804
Size: 6613
FspI TGC'GCA
Cuts at: 2405 5119 2405
Size: 2714 3899
HaeII r_GCGC'y
Cuts at: 1678 2044 2095 2143 2210 3118 5760 1678
Size: 366 51 48 67 908 2642 2531
Fragments arranged by size:
2642 2531 908 366 67 51 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2126 2158 2415 2447 2704
Size: 32 135 32 554 32 257 32 257
Cuts at: 2704 2736 2771 2803 2816 2848 3134 3166 3296
Size: 32 35 32 13 32 286 32 130
Cuts at: 3296 3328 5254 5286 5328 5360 1373
Size: 32 1926 32 42 32 2626
Fragments arranged by size:
2626 1926 554 286 257 257 135 130
42 35 32 32 32 32 32 32
32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3957 4203 669
Size: 988 281 2019 246 3079
Fragments arranged by size:
3079 2019 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6613
HpaI GTT'AAC
Cuts at: 4203 4203
Size: 6613
KasI G'GCGC_C
Cuts at: 2040 2091 2040
Size: 51 6562
KpnI G_GTAC'C
Cuts at: 3938 3938
Size: 6613
MfeI C'AATT_G
Cuts at: 4212 4212
Size: 6613
MluI A'CGCG_T
Cuts at: 6489 6489
Size: 6613
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3000 3264 5727 5911 1305
Size: 1695 264 2463 184 2007
Fragments arranged by size:
2463 2007 1695 264 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3875 10
Size: 54 1425 343 2043 2748
Fragments arranged by size:
2748 2043 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2810 3350 4571 4930 5089 518
Size: 648 1644 540 1221 359 159 2042
Fragments arranged by size:
2042 1644 1221 648 540 359 159
MspA1I CmG'CkG
Cuts at: 1606 2054 2567 2738 3070 3569 4029 4706 5854
Size: 448 513 171 332 499 460 677 1148
Cuts at: 5854 6099 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 513 499 460 448 332
245 171
NaeI GCC'GGC
Cuts at: 2151 2151
Size: 6613
NarI GG'CG_CC
Cuts at: 2041 2092 2041
Size: 51 6562
NcoI C'CATG_G
Cuts at: 513 1065 2004 3682 513
Size: 552 939 1678 3444
Fragments arranged by size:
3444 1678 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6613
NgoMIV G'CCGG_C
Cuts at: 2149 2149
Size: 6613
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6613
NotI GC'GGCC_GC
Cuts at: 4060 4060
Size: 6613
NspI r_CATG'y
Cuts at: 1132 1229 3971 5516 1132
Size: 97 2742 1545 2229
Fragments arranged by size:
2742 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3199 3209 1348
Size: 1851 10 4752
Fragments arranged by size:
4752 1851 10
PciI A'CATG_T
Cuts at: 5512 5512
Size: 6613
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2057 3802 1108
Size: 949 1745 3919
Fragments arranged by size:
3919 1745 949
PfoI T'CCnGG_A
Cuts at: 2997 2997
Size: 6613
PmeI GTTT'AAAC
Cuts at: 3917 3917
Size: 6613
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4682 4714 6195 6227 4682
Size: 32 1481 32 5068
Fragments arranged by size:
5068 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2779 3007 3268 2779
Size: 228 261 6124
Fragments arranged by size:
6124 261 228
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6613
PsiI TTA'TAA
Cuts at: 4183 4183
Size: 6613
PspOMI G'GGCC_C
Cuts at: 3729 3729
Size: 6613
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2920 2952 2920
Size: 32 6581
PstI C_TGCA'G
Cuts at: 830 2337 2488 3075 3406 3642 3965 830
Size: 1507 151 587 331 236 323 3478
Fragments arranged by size:
3478 1507 587 331 323 236 151
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6613
PvuII CAG'CTG
Cuts at: 2054 2738 3070 2054
Size: 684 332 5597
Fragments arranged by size:
5597 684 332
SacI G_AGCT'C
Cuts at: 2789 2789
Size: 6613
SalI G'TCGA_C
Cuts at: 1936 3955 1936
Size: 2019 4594
SbfI CC_TGCA'GG
Cuts at: 3965 3965
Size: 6613
ScaI AGT'ACT
Cuts at: 2929 4861 6573 2929
Size: 1932 1712 2969
Fragments arranged by size:
2969 1932 1712
SexAI A'CCwGG_T
Cuts at: 3411 3411
Size: 6613
SfcI C'TryA_G
Cuts at: 826 1045 2333 2456 2484 3071 3272 3402 3638
Size: 219 1288 123 28 587 201 130 236
Cuts at: 3638 3673 3961 5096 5777 5968 826
Size: 35 288 1135 681 191 1471
Fragments arranged by size:
1471 1288 1135 681 587 288 236 219
201 191 130 123 35 28
SfoI GGC'GCC
Cuts at: 2042 2093 2042
Size: 51 6562
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6613
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6613
SmaI CCC'GGG
Cuts at: 1872 3940 1872
Size: 2068 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3724 4717 5618 5880 820
Size: 197 926 1781 993 901 262 1553
Fragments arranged by size:
1781 1553 993 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4404 493
Size: 3911 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6613
SphI G_CATG'C
Cuts at: 1132 3971 1132
Size: 2839 3774
SspI AAT'ATT
Cuts at: 5 52 4537 5
Size: 47 4485 2081
Fragments arranged by size:
4485 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6613
StyI C'CwwG_G
Cuts at: 513 1065 2004 2079 3264 3438 3457 3682 4052
Size: 552 939 75 1185 174 19 225 370
Cuts at: 4052 513
Size: 3074
Fragments arranged by size:
3074 1185 939 552 370 225 174 75
19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3222 3512 4640 4657 4810 4995 5440
Size: 1255 1642 290 1128 17 153 185 445
Cuts at: 5440 325
Size: 1498
Fragments arranged by size:
1642 1498 1255 1128 445 290 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2927 3366 3508
Size: 274 80 33 51 1443 950 439 142
Cuts at: 3508 4859 6571 96
Size: 1351 1712 138
Fragments arranged by size:
1712 1443 1351 950 439 274 142 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2308 2340 3645 3677 6573 6605 2308
Size: 32 1305 32 2896 32 2316
Fragments arranged by size:
2896 2316 1305 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6613
XbaI T'CTAG_A
Cuts at: 3949 3949
Size: 6613
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2536 3566 2536
Size: 1030 5583
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6613
XmaI C'CCGG_G
Cuts at: 1870 3938 1870
Size: 2068 4545
XmnI GAAnn'nnTTC
Cuts at: 4742 4742
Size: 6613
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4421 276
Size: 53 83 186 936 2887 2468
Fragments arranged by size:
2887 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI
PacI PmlI PspXI RsrII SacII SanDI SapI SfiI
SrfI SwaI