Restriction map for
pFN21AA2014
(Circular) (Six-base) MAPSORT of: pFN21AA2014.seq Check: 1113 from: 1 to: 7786
pFN21AA2014 7786 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3039 3097 3039
Size: 58 7728
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2676 5596 278
Size: 53 83 186 936 1140 2920 2468
Fragments arranged by size:
2920 2468 1140 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5129 1937
Size: 3192 4594
Acc65I G'GTAC_C
Cuts at: 4976 5107 4976
Size: 131 7655
AclI AA'CG_TT
Cuts at: 1563 5913 6286 1563
Size: 4350 373 3063
Fragments arranged by size:
4350 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2660 3754 4138 4313 5849 7233 7501 1632
Size: 1028 1094 384 175 1536 1384 268 1917
Fragments arranged by size:
1917 1536 1384 1094 1028 384 268 175
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7589
AflIII A'CryG_T
Cuts at: 1203 3135 6685 7662 1203
Size: 1932 3550 977 1327
Fragments arranged by size:
3550 1932 1327 977
AgeI A'CCGG_T
Cuts at: 6617 6617
Size: 7786
AhdI GACnn_n'nnGTC
Cuts at: 6515 6515
Size: 7786
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7786
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2918 3870 3915 4263 1184
Size: 1734 952 45 348 4707
Fragments arranged by size:
4707 1734 952 348 45
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2099 2131 2099
Size: 32 7754
AlwNI CAG_nnn'CTG
Cuts at: 4070 4114 4291 4381 5529 7101 4070
Size: 44 177 90 1148 1572 4755
Fragments arranged by size:
4755 1572 1148 177 90 44
ApaI G_GGCC'C
Cuts at: 2638 3854 2638
Size: 1216 6570
ApaLI G'TGCA_C
Cuts at: 2996 3356 5843 6999 2996
Size: 360 2487 1156 3783
Fragments arranged by size:
3783 2487 1156 360
ApoI r'AATT_y
Cuts at: 1493 2384 5095 5321 5473 7582 1493
Size: 891 2711 226 152 2109 1697
Fragments arranged by size:
2711 2109 1697 891 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6340 160
Size: 872 5308 1606
Fragments arranged by size:
5308 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7786
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 5067 5111 1870
Size: 73 92 3032 44 4545
Fragments arranged by size:
4545 3032 92 73 44
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2668 2701 2668
Size: 33 7753
BamHI G'GATC_C
Cuts at: 5116 5483 5116
Size: 367 7419
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4932 4976 5107 6562 618
Size: 325 217 627 3145 44 131 1455 1842
Fragments arranged by size:
3145 1842 1455 627 325 217 131 44
BanII G_rGCy'C
Cuts at: 1391 2046 2097 2135 2638 3854 5074 5105 1391
Size: 655 51 38 503 1216 1220 31 4072
Fragments arranged by size:
4072 1220 1216 655 503 51 38 31
BbeI G_GCGC'C
Cuts at: 4936 4936
Size: 7786
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3715 3760 4272 928
Size: 971 1816 45 512 4442
Fragments arranged by size:
4442 1816 971 512 45
BbvCI CC'TCA_GC
Cuts at: 3978 4071 4131 4677 3978
Size: 93 60 546 7087
Fragments arranged by size:
7087 546 93 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2509 2543 5977 6011 1903
Size: 34 572 34 3434 34 3678
Fragments arranged by size:
3678 3434 572 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2696 4390 5678 6888 2696
Size: 1694 1288 1210 3594
Fragments arranged by size:
3594 1694 1288 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7786
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2558 4673 6397
Size: 107 122 71 631 605 886 2115 1724
Cuts at: 6397 136
Size: 1525
Fragments arranged by size:
2115 1724 1525 886 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2346 3792 3984 7781 2346
Size: 1446 192 3797 2351
Fragments arranged by size:
3797 2351 1446 192
BlpI GC'TnA_GC
Cuts at: 2129 5203 2129
Size: 3074 4712
Bme1580I G_kGCm'C
Cuts at: 1790 2638 3000 3360 3854 5847 7003 1790
Size: 848 362 360 494 1993 1156 2573
Fragments arranged by size:
2573 1993 1156 848 494 362 360
BmgBI CAC'GTC
Cuts at: 1318 2680 4546 1318
Size: 1362 1866 4558
Fragments arranged by size:
4558 1866 1362
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2274 2437 4331
Size: 416 53 83 399 24 857 163 1894
Cuts at: 4331 4745 5049 5517 6475 442
Size: 414 304 468 958 1753
Fragments arranged by size:
1894 1753 958 857 468 416 414 399
304 163 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7786
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 7786
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2126 2133 2246 2363 2536 3209 3458
Size: 797 90 7 113 117 173 673 249
Cuts at: 3458 3510 4136 4358 4547 4640 5058 6446 1239
Size: 52 626 222 189 93 418 1388 2579
Fragments arranged by size:
2579 1388 797 673 626 418 249 222
189 173 117 113 93 90 52 7
Bpu10I CC'TnA_GC
Cuts at: 2091 3978 4071 4131 4677 4848 2091
Size: 1887 93 60 546 171 5029
Fragments arranged by size:
5029 1887 546 171 93 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5911 6776 7074 5911
Size: 865 298 6623
Fragments arranged by size:
6623 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3164 6449 882
Size: 617 1665 3285 2219
Fragments arranged by size:
3285 2219 1665 617
BsaAI yAC'GTr
Cuts at: 493 1204 5577 493
Size: 711 4373 2702
Fragments arranged by size:
4373 2702 711
BsaBI GATnn'nnATC
Cuts at: 7780 7780
Size: 7786
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2673 4262 4665 4933
Size: 53 83 186 936 1140 1589 403 268
Cuts at: 4933 5593 5975 6610 275
Size: 660 382 635 1451
Fragments arranged by size:
1589 1451 1140 936 660 635 403 382
268 186 83 53
BsaWI w'CCGG_w
Cuts at: 2508 2607 2934 4413 4614 6219 6617 6891 7038
Size: 99 327 1479 201 1605 398 274 147
Cuts at: 7038 7449 2508
Size: 411 2845
Fragments arranged by size:
2845 1605 1479 411 398 327 274 201
147 99
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2099 2129 3203 3233 4717
Size: 30 165 30 696 30 1074 30 1484
Cuts at: 4717 4747 1178
Size: 30 4217
Fragments arranged by size:
4217 1484 1074 696 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2320 2859 3077 3215 3224 3404 3557 3995
Size: 1134 539 218 138 9 180 153 438
Cuts at: 3995 4133 4694 4835 1186
Size: 138 561 141 4137
Fragments arranged by size:
4137 1134 561 539 438 218 180 153
141 138 138 9
BseYI C'CCAG_C
Cuts at: 2546 3052 3619 4176 4555 4884 6989 7485 2546
Size: 506 567 557 379 329 2105 496 2847
Fragments arranged by size:
2847 2105 567 557 506 496 379 329
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2333 2888 3131 3627 4478 4609 5031
Size: 573 597 555 243 496 851 131 422
Cuts at: 5031 1163
Size: 3918
Fragments arranged by size:
3918 851 597 573 555 496 422 243
131
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5236 5997 7025 1540
Size: 275 186 3235 761 1028 2301
Fragments arranged by size:
3235 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2135 2607 3000 3360 5847 5932 7003 2135
Size: 472 393 360 2487 85 1071 2918
Fragments arranged by size:
2918 2487 1071 472 393 360 85
BsmI GAATG_Cn'
Cuts at: 5297 5390 5297
Size: 93 7693
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2097 2135 2607 2638 3000 3360
Size: 399 256 51 38 472 31 362 360
Cuts at: 3360 3854 5074 5105 5847 5932 7003 1391
Size: 494 1220 31 742 85 1071 2174
Fragments arranged by size:
2174 1220 1071 742 494 472 399 362
360 256 85 51 38 31 31
BspEI T'CCGG_A
Cuts at: 2934 4614 7449 2934
Size: 1680 2835 3271
Fragments arranged by size:
3271 2835 1680
BspHI T'CATG_A
Cuts at: 2706 5675 7405 2706
Size: 2969 1730 3087
Fragments arranged by size:
3087 2969 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2151 2511 2544 3039 3097 4230 5141 844
Size: 1307 360 33 495 58 1133 911 3489
Fragments arranged by size:
3489 1307 1133 911 495 360 58 33
BsrBI CCG'CTC
Cuts at: 1394 1823 3972 4099 4105 5673 1394
Size: 429 2149 127 6 1568 3507
Fragments arranged by size:
3507 2149 1568 429 127 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 4254 6281 6455 65
Size: 1183 3006 2027 174 1396
Fragments arranged by size:
3006 2027 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4950 6430 6617 1700
Size: 110 3140 1480 187 2869
Fragments arranged by size:
3140 2869 1480 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7786
BssHII G'CGCG_C
Cuts at: 1925 2591 1925
Size: 666 7120
BssSI C'ACGA_G
Cuts at: 5846 6858 5846
Size: 1012 6774
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3364 4492 794
Size: 2570 1128 4088
Fragments arranged by size:
4088 2570 1128
BstBI TT'CG_AA
Cuts at: 3682 6590 3682
Size: 2908 4878
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7786
BstXI CCAn_nnnn'nTGG
Cuts at: 3758 3943 4864 3758
Size: 185 921 6680
Fragments arranged by size:
6680 921 185
BstYI r'GATC_y
Cuts at: 1972 2197 2346 3792 3808 3984 4384 5116 5483
Size: 225 149 1446 16 176 400 732 367
Cuts at: 5483 5868 5885 7337 7423 7435 7781 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1446 732 400 385 367 346
225 176 149 86 17 16 12
Bsu36I CC'TnA_GG
Cuts at: 3540 3540
Size: 7786
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6295
Fragments arranged by size:
6295 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4423 487
Size: 683 56 3197 3850
Fragments arranged by size:
3850 3197 683 56
BtsI GCAGTG_nn'
Cuts at: 4424 4524 5005 5302 6096 6116 4424
Size: 100 481 297 794 20 6094
Fragments arranged by size:
6094 794 481 297 100 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7786
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7751
DraI TTT'AAA
Cuts at: 5090 5090
Size: 7786
DraIII CAC_nnn'GTG
Cuts at: 3004 3355 3004
Size: 351 7435
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2678 4607 6793 809
Size: 507 1362 1929 2186 1802
Fragments arranged by size:
2186 1929 1802 1362 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3462 3945 3967
Size: 54 1425 50 275 18 1632 483 22
Cuts at: 3967 5233 6122 7751 8
Size: 1266 889 1629 43
Fragments arranged by size:
1632 1629 1425 1266 889 483 275 54
50 43 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 5233 1537
Size: 275 3421 4090
Fragments arranged by size:
4090 3421 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2358 3561 4157 5716 1353
Size: 1005 1203 596 1559 3423
Fragments arranged by size:
3423 1559 1203 1005 596
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3564 6373 6747 6893 387
Size: 1064 138 1975 2809 374 146 1280
Fragments arranged by size:
2809 1975 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2133 2133
Size: 7786
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2126 2133 2246 2363 2536 2660
Size: 393 404 90 7 113 117 173 124
Cuts at: 2660 3209 3458 3510 3754 4136 4138 4313 4358
Size: 549 249 52 244 382 2 175 45
Cuts at: 4358 4547 4640 5058 5849 6446 7233 7501 1239
Size: 189 93 418 791 597 787 268 1524
Fragments arranged by size:
1524 791 787 597 549 418 404 393
382 268 249 244 189 175 173 124
117 113 93 90 52 45 7 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2518 3191 3959 2518
Size: 673 768 6345
Fragments arranged by size:
6345 768 673
EcoO109I rG'GnC_Cy
Cuts at: 2032 2533 2634 2635 3433 3457 3473 3547 3802
Size: 501 101 1 798 24 16 74 255
Cuts at: 3802 3850 3851 5078 2032
Size: 48 1 1227 4740
Fragments arranged by size:
4740 1227 798 501 255 101 74 48
24 16 1 1
EcoRI G'AATT_C
Cuts at: 5095 5473 5095
Size: 378 7408
FspI TGC'GCA
Cuts at: 6292 6292
Size: 7786
HaeII r_GCGC'y
Cuts at: 1678 2025 2553 4936 6933 1678
Size: 347 528 2383 1997 2531
Fragments arranged by size:
2531 2383 1997 528 347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2417 2449 3203 3235 4369
Size: 32 135 32 845 32 754 32 1134
Cuts at: 4369 4388 4401 4420 4717 4749 6427 6459 6501
Size: 19 13 19 297 32 1678 32 42
Cuts at: 6501 6533 1373
Size: 32 2626
Fragments arranged by size:
2626 1678 1134 845 754 297 135 42
32 32 32 32 32 32 32 19
19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2322 3660 4173 5130 5376 669
Size: 988 281 384 1338 513 957 246 3079
Fragments arranged by size:
3079 1338 988 957 513 384 281 246
HindIII A'AGCT_T
Cuts at: 748 3236 748
Size: 2488 5298
HpaI GTT'AAC
Cuts at: 5376 5376
Size: 7786
KasI G'GCGC_C
Cuts at: 4932 4932
Size: 7786
KpnI G_GTAC'C
Cuts at: 4980 5111 4980
Size: 131 7655
MfeI C'AATT_G
Cuts at: 5385 5385
Size: 7786
MluI A'CGCG_T
Cuts at: 7662 7662
Size: 7786
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3138 3186 3531 3900 4284 6900 7084 1305
Size: 1833 48 345 369 384 2616 184 2007
Fragments arranged by size:
2616 2007 1833 384 369 345 184 48
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3464 3947 10
Size: 54 1425 343 1632 483 3849
Fragments arranged by size:
3849 1632 1425 483 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2748 2993 5744 6103 6262 518
Size: 648 1582 245 2751 359 159 2042
Fragments arranged by size:
2751 2042 1582 648 359 245 159
MspA1I CmG'CkG
Cuts at: 1606 2828 3292 3301 4130 4660 4843 4888 5202
Size: 1222 464 9 829 530 183 45 314
Cuts at: 5202 5879 7027 7272 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1222 1148 829 677 530 464 314
245 183 45 9
NarI GG'CG_CC
Cuts at: 4933 4933
Size: 7786
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6295
Fragments arranged by size:
6295 939 552
NdeI CA'TA_TG
Cuts at: 387 3334 387
Size: 2947 4839
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7786
NotI GC'GGCC_GC
Cuts at: 5233 5233
Size: 7786
NspI r_CATG'y
Cuts at: 1132 1229 2977 3218 3499 5144 6689 1132
Size: 97 1748 241 281 1645 1545 2229
Fragments arranged by size:
2229 1748 1645 1545 281 241 97
PasI CC'CwG_GG
Cuts at: 1348 2624 1348
Size: 1276 6510
PciI A'CATG_T
Cuts at: 6685 6685
Size: 7786
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4183 4381 5017 1108
Size: 3075 198 636 3877
Fragments arranged by size:
3877 3075 636 198
PfoI T'CCnGG_A
Cuts at: 2513 4212 2513
Size: 1699 6087
PmeI GTTT'AAAC
Cuts at: 5090 5090
Size: 7786
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2099 2131 5855 5887 7368 7400 2099
Size: 32 3724 32 1481 32 2485
Fragments arranged by size:
3724 2485 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2032 2533 3547 3802 2032
Size: 501 1014 255 6016
Fragments arranged by size:
6016 1014 501 255
PshAI GACnn'nnGTC
Cuts at: 1613 3612 1613
Size: 1999 5787
PsiI TTA'TAA
Cuts at: 5356 5356
Size: 7786
PspOMI G'GGCC_C
Cuts at: 2634 3850 2634
Size: 1216 6570
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2702 2734 2702
Size: 32 7754
PstI C_TGCA'G
Cuts at: 830 2541 3036 3075 3108 3481 3992 4227 4516
Size: 1711 495 39 33 373 511 235 289
Cuts at: 4516 5138 830
Size: 622 3478
Fragments arranged by size:
3478 1711 622 511 495 373 289 235
39 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7786
PvuII CAG'CTG
Cuts at: 2828 3292 4130 4843 2828
Size: 464 838 713 5771
Fragments arranged by size:
5771 838 713 464
SacI G_AGCT'C
Cuts at: 2135 2135
Size: 7786
SalI G'TCGA_C
Cuts at: 1936 5128 1936
Size: 3192 4594
SanDI GG'GwC_CC
Cuts at: 2032 2032
Size: 7786
SapI GCTCTTCn'nnn_
Cuts at: 4157 4157
Size: 7786
SbfI CC_TGCA'GG
Cuts at: 5138 5138
Size: 7786
ScaI AGT'ACT
Cuts at: 2331 6034 7746 2331
Size: 3703 1712 2371
Fragments arranged by size:
3703 2371 1712
SexAI A'CCwGG_T
Cuts at: 2730 3351 2730
Size: 621 7165
SfcI C'TryA_G
Cuts at: 826 1045 2537 3032 3071 3104 3296 3477 3988
Size: 219 1492 495 39 33 192 181 511
Cuts at: 3988 4223 4512 5134 6269 6950 7141 826
Size: 235 289 622 1135 681 191 1471
Fragments arranged by size:
1492 1471 1135 681 622 511 495 289
235 219 192 191 181 39 33
SfoI GGC'GCC
Cuts at: 4934 4934
Size: 7786
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7786
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7786
SmaI CCC'GGG
Cuts at: 1872 2037 5113 1872
Size: 165 3076 4545
Fragments arranged by size:
4545 3076 165
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5890 6791 7053 820
Size: 197 926 3947 901 262 1553
Fragments arranged by size:
3947 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5577 493
Size: 5084 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7786
SphI G_CATG'C
Cuts at: 1132 2977 3499 5144 1132
Size: 1845 522 1645 3774
Fragments arranged by size:
3774 1845 1645 522
SspI AAT'ATT
Cuts at: 5 52 2953 5710 5
Size: 47 2901 2757 2081
Fragments arranged by size:
2901 2757 2081 47
StuI AGG'CCT
Cuts at: 1517 2409 3395 4685 5001 1517
Size: 892 986 1290 316 4302
Fragments arranged by size:
4302 1290 986 892 316
StyI C'CwwG_G
Cuts at: 513 1065 2004 2792 4941 5225 513
Size: 552 939 788 2149 284 3074
Fragments arranged by size:
3074 2149 939 788 552 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2361 2365 2560 5813 5830 5983 6168
Size: 1255 781 4 195 3253 17 153 185
Cuts at: 6168 6613 325
Size: 445 1498
Fragments arranged by size:
3253 1498 1255 781 445 195 185 153
17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2329 2416 6032
Size: 274 80 33 51 1443 352 87 3616
Cuts at: 6032 7744 96
Size: 1712 138
Fragments arranged by size:
3616 1712 1443 352 274 138 87 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7746 7778 7746
Size: 32 7754
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7786
XbaI T'CTAG_A
Cuts at: 5122 5122
Size: 7786
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 4184 2011
Size: 2173 5613
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7786
XmaI C'CCGG_G
Cuts at: 1870 2035 5111 1870
Size: 165 3076 4545
Fragments arranged by size:
4545 3076 165
XmnI GAAnn'nnTTC
Cuts at: 2297 5915 2297
Size: 3618 4168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2674 5594 276
Size: 53 83 186 936 1140 2920 2468
Fragments arranged by size:
2920 2468 1140 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BsiWI BsmBI BstZ17I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI RsrII SacII SfiI SrfI SwaI