Restriction map for
pFN21AA2001
(Circular) (Six-base) MAPSORT of: pFN21AA2001.seq Check: 7666 from: 1 to: 6412
pFN21AA2001 6412 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3643 4222 278
Size: 53 83 186 936 2107 579 2468
Fragments arranged by size:
2468 2107 936 579 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2487 3755 1937
Size: 550 1268 4594
Fragments arranged by size:
4594 1268 550
Acc65I G'GTAC_C
Cuts at: 3733 3733
Size: 6412
AclI AA'CG_TT
Cuts at: 1563 4539 4912 1563
Size: 2976 373 3063
Fragments arranged by size:
3063 2976 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4475 5859 6127 1632
Size: 2843 1384 268 1917
Fragments arranged by size:
2843 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6215
AflIII A'CryG_T
Cuts at: 1203 2707 3432 5311 6288 1203
Size: 1504 725 1879 977 1327
Fragments arranged by size:
1879 1504 1327 977 725
AgeI A'CCGG_T
Cuts at: 5243 5243
Size: 6412
AhdI GACnn_n'nnGTC
Cuts at: 5141 5141
Size: 6412
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6412
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6412
AlwNI CAG_nnn'CTG
Cuts at: 2785 3335 4155 5727 2785
Size: 550 820 1572 3470
Fragments arranged by size:
3470 1572 820 550
ApaI G_GGCC'C
Cuts at: 2355 2355
Size: 6412
ApaLI G'TGCA_C
Cuts at: 4469 5625 4469
Size: 1156 5256
ApoI r'AATT_y
Cuts at: 1493 2498 2684 2849 3702 3721 3947 4099 6208
Size: 1005 186 165 853 19 226 152 2109
Cuts at: 6208 1493
Size: 1697
Fragments arranged by size:
2109 1697 1005 853 226 186 165 152
19
AseI AT'TA_AT
Cuts at: 160 1032 4966 160
Size: 872 3934 1606
Fragments arranged by size:
3934 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6412
AvaI C'yCGr_G
Cuts at: 1870 1943 2111 2243 2549 3737 1870
Size: 73 168 132 306 1188 4545
Fragments arranged by size:
4545 1188 306 168 132 73
BamHI G'GATC_C
Cuts at: 3325 3742 4109 3325
Size: 417 367 5628
Fragments arranged by size:
5628 417 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2440 2555 3563 3733 5188
Size: 325 217 627 653 115 1008 170 1455
Cuts at: 5188 618
Size: 1842
Fragments arranged by size:
1842 1455 1008 653 627 325 217 170
115
BanII G_rGCy'C
Cuts at: 1391 2122 2292 2355 2469 2868 3731 1391
Size: 731 170 63 114 399 863 4072
Fragments arranged by size:
4072 863 731 399 170 114 63
BbeI G_GCGC'C
Cuts at: 2559 2559
Size: 6412
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3054 3643 928
Size: 971 1155 589 3697
Fragments arranged by size:
3697 1155 971 589
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4603 4637 1903
Size: 34 2666 34 3678
Fragments arranged by size:
3678 2666 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4304 5514 4304
Size: 1210 5202
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6412
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3561 5023 136
Size: 107 122 71 631 605 1889 1462 1525
Fragments arranged by size:
1889 1525 1462 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6407 6407
Size: 6412
BlpI GC'TnA_GC
Cuts at: 3829 3829
Size: 6412
Bme1580I G_kGCm'C
Cuts at: 1790 2190 2355 4473 5629 1790
Size: 400 165 2118 1156 2573
Fragments arranged by size:
2573 2118 1156 400 165
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6412
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2477 2617 4143
Size: 416 53 83 399 24 1060 140 1526
Cuts at: 4143 5101 442
Size: 958 1753
Fragments arranged by size:
1753 1526 1060 958 416 399 140 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2140 2468 2985 5072 1239
Size: 851 50 328 517 2087 2579
Fragments arranged by size:
2579 2087 851 517 328 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2720 4537 5402 5700 2720
Size: 1817 865 298 3432
Fragments arranged by size:
3432 1817 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2472 2510 3335 5075 882
Size: 617 973 38 825 1740 2219
Fragments arranged by size:
2219 1740 973 825 617 38
BsaAI yAC'GTr
Cuts at: 493 1204 2708 4203 493
Size: 711 1504 1495 2702
Fragments arranged by size:
2702 1504 1495 711
BsaBI GATnn'nnATC
Cuts at: 6406 6406
Size: 6412
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 2556 3640 4219
Size: 53 83 186 936 482 541 1084 579
Cuts at: 4219 4601 5236 275
Size: 382 635 1451
Fragments arranged by size:
1451 1084 936 635 579 541 482 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4845 5243 5517 5664 6075 4845
Size: 398 274 147 411 5182
Fragments arranged by size:
5182 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2469 2499 1178
Size: 30 165 30 1066 30 5091
Fragments arranged by size:
5091 1066 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2216 3149 3152 3155 3200 3203 3206 3233
Size: 1030 933 3 3 45 3 3 27
Cuts at: 3233 3236 3239 3242 3263 3353 3374 3377 1186
Size: 3 3 3 21 90 21 3 4221
Fragments arranged by size:
4221 1030 933 90 45 27 21 21
3 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 5615 6111 5615
Size: 496 5916
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5839
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3862 4623 5651 1540
Size: 275 186 1861 761 1028 2301
Fragments arranged by size:
2301 1861 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2868 4473 4558 5629 2868
Size: 1605 85 1071 3651
Fragments arranged by size:
3651 1605 1071 85
BsmI GAATG_Cn'
Cuts at: 2095 3923 4016 2095
Size: 1828 93 4491
Fragments arranged by size:
4491 1828 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2122 2190 2292 2355 2469 2868 3731
Size: 399 332 68 102 63 114 399 863
Cuts at: 3731 4473 4558 5629 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 863 742 399 399 332 114
102 85 68 63
BspEI T'CCGG_A
Cuts at: 6075 6075
Size: 6412
BspHI T'CATG_A
Cuts at: 4301 6031 4301
Size: 1730 4682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3767 844
Size: 2923 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2452 2563 2959 4299 1394
Size: 429 629 111 396 1340 3507
Fragments arranged by size:
3507 1340 629 429 396 111
BsrDI GCAATG_nn'
Cuts at: 65 1248 2155 4907 5081 65
Size: 1183 907 2752 174 1396
Fragments arranged by size:
2752 1396 1183 907 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2268 2389 5056 5243 1700
Size: 110 458 121 2667 187 2869
Fragments arranged by size:
2869 2667 458 187 121 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6412
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6412
BssSI C'ACGA_G
Cuts at: 3693 4472 5484 3693
Size: 779 1012 4621
Fragments arranged by size:
4621 1012 779
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6412
BstBI TT'CG_AA
Cuts at: 2971 3634 5216 2971
Size: 663 1582 4167
Fragments arranged by size:
4167 1582 663
BstEII G'GTnAC_C
Cuts at: 1184 2620 3464 1184
Size: 1436 844 4132
Fragments arranged by size:
4132 1436 844
BstXI CCAn_nnnn'nTGG
Cuts at: 3449 3449
Size: 6412
BstYI r'GATC_y
Cuts at: 1972 3325 3742 4109 4494 4511 5963 6049 6061
Size: 1353 417 367 385 17 1452 86 12
Cuts at: 6061 6407 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1353 417 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5860
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5673
Fragments arranged by size:
5673 683 56
BtsI GCAGTG_nn'
Cuts at: 3928 4722 4742 3928
Size: 794 20 5598
Fragments arranged by size:
5598 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6412
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6377
DraI TTT'AAA
Cuts at: 3716 3716
Size: 6412
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5419 809
Size: 507 4103 1802
Fragments arranged by size:
4103 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 2171 2886
Size: 54 1425 50 275 18 294 47 715
Cuts at: 2886 3697 3859 4748 6377 8
Size: 811 162 889 1629 43
Fragments arranged by size:
1629 1425 889 811 715 294 275 162
54 50 47 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3859 1537
Size: 275 2047 4090
Fragments arranged by size:
4090 2047 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3021 3171 3192 4342 1353
Size: 1668 150 21 1150 3423
Fragments arranged by size:
3423 1668 1150 150 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2391 3647 4999 5373 5519 387
Size: 1064 138 802 1256 1352 374 146 1280
Fragments arranged by size:
1352 1280 1256 1064 802 374 146 138
EcoICRI GAG'CTC
Cuts at: 2866 2866
Size: 6412
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2140 2468 2985 4475 5072 5859
Size: 393 458 50 328 517 1490 597 787
Cuts at: 5859 6127 1239
Size: 268 1524
Fragments arranged by size:
1524 1490 787 597 517 458 393 328
268 50
EcoNI CCTnn'n_nnAGG
Cuts at: 2126 2198 2126
Size: 72 6340
EcoRI G'AATT_C
Cuts at: 2498 2684 2849 3702 3721 4099 2498
Size: 186 165 853 19 378 4811
Fragments arranged by size:
4811 853 378 186 165 19
FspI TGC'GCA
Cuts at: 4918 4918
Size: 6412
HaeII r_GCGC'y
Cuts at: 1678 2396 2559 5559 1678
Size: 718 163 3000 2531
Fragments arranged by size:
3000 2531 718 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2285 2317 2349 2469 2501
Size: 32 135 32 713 32 32 120 32
Cuts at: 2501 5053 5085 5127 5159 1373
Size: 2552 32 42 32 2626
Fragments arranged by size:
2626 2552 713 135 120 42 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2877 3756 4002 669
Size: 988 281 939 879 246 3079
Fragments arranged by size:
3079 988 939 879 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6412
HpaI GTT'AAC
Cuts at: 4002 4002
Size: 6412
KasI G'GCGC_C
Cuts at: 2555 2555
Size: 6412
KpnI G_GTAC'C
Cuts at: 3737 3737
Size: 6412
MfeI C'AATT_G
Cuts at: 4011 4011
Size: 6412
MluI A'CGCG_T
Cuts at: 3432 6288 3432
Size: 2856 3556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2826 5526 5710 1305
Size: 1521 2700 184 2007
Fragments arranged by size:
2700 2007 1521 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2173 10
Size: 54 1425 343 341 4249
Fragments arranged by size:
4249 1425 343 341 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4370 4729 4888 518
Size: 648 3204 359 159 2042
Fragments arranged by size:
3204 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2169 2357 2446 3828 4505 5653 5898 1606
Size: 563 188 89 1382 677 1148 245 2120
Fragments arranged by size:
2120 1382 1148 677 563 245 188 89
NaeI GCC'GGC
Cuts at: 2270 2391 2270
Size: 121 6291
NarI GG'CG_CC
Cuts at: 2556 2556
Size: 6412
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5860
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6412
NgoMIV G'CCGG_C
Cuts at: 2268 2389 2268
Size: 121 6291
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6412
NotI GC'GGCC_GC
Cuts at: 3859 3859
Size: 6412
NspI r_CATG'y
Cuts at: 1132 1229 2648 3770 5315 1132
Size: 97 1419 1122 1545 2229
Fragments arranged by size:
2229 1545 1419 1122 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6412
PciI A'CATG_T
Cuts at: 5311 5311
Size: 6412
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2607 1108
Size: 1499 4913
PfoI T'CCnGG_A
Cuts at: 2328 2664 2328
Size: 336 6076
PmeI GTTT'AAAC
Cuts at: 3716 3716
Size: 6412
PmlI CAC'GTG
Cuts at: 2708 2708
Size: 6412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4481 4513 5994 6026 4481
Size: 32 1481 32 4867
Fragments arranged by size:
4867 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6412
PsiI TTA'TAA
Cuts at: 3982 3982
Size: 6412
PspOMI G'GGCC_C
Cuts at: 2351 2351
Size: 6412
PstI C_TGCA'G
Cuts at: 830 3764 830
Size: 2934 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6412
SacI G_AGCT'C
Cuts at: 2868 2868
Size: 6412
SalI G'TCGA_C
Cuts at: 1936 3754 1936
Size: 1818 4594
SbfI CC_TGCA'GG
Cuts at: 3764 3764
Size: 6412
ScaI AGT'ACT
Cuts at: 3032 4660 6372 3032
Size: 1628 1712 3072
Fragments arranged by size:
3072 1712 1628
SfcI C'TryA_G
Cuts at: 826 1045 3034 3580 3760 4895 5576 5767 826
Size: 219 1989 546 180 1135 681 191 1471
Fragments arranged by size:
1989 1471 1135 681 546 219 191 180
SfoI GGC'GCC
Cuts at: 2557 2557
Size: 6412
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6412
SgrAI Cr'CCGG_yG
Cuts at: 1700 2389 1700
Size: 689 5723
SmaI CCC'GGG
Cuts at: 1872 2245 2551 3739 1872
Size: 373 306 1188 4545
Fragments arranged by size:
4545 1188 373 306
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2735 4516 5417 5679 820
Size: 197 926 792 1781 901 262 1553
Fragments arranged by size:
1781 1553 926 901 792 262 197
SnaBI TAC'GTA
Cuts at: 493 4203 493
Size: 3710 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6412
SphI G_CATG'C
Cuts at: 1132 2648 3770 1132
Size: 1516 1122 3774
Fragments arranged by size:
3774 1516 1122
SspI AAT'ATT
Cuts at: 5 52 4336 5
Size: 47 4284 2081
Fragments arranged by size:
4284 2081 47
StuI AGG'CCT
Cuts at: 1517 2841 3555 1517
Size: 1324 714 4374
Fragments arranged by size:
4374 1324 714
StyI C'CwwG_G
Cuts at: 513 1065 2601 3081 3851 513
Size: 552 1536 480 770 3074
Fragments arranged by size:
3074 1536 770 552 480
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2608 2639 3476 4439 4456 4609 4794
Size: 1255 1028 31 837 963 17 153 185
Cuts at: 4794 5239 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1028 963 837 445 185 153
31 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3030 4658 6370
Size: 274 80 33 51 1443 1053 1628 1712
Cuts at: 6370 96
Size: 138
Fragments arranged by size:
1712 1628 1443 1053 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3422 3454 6372 6404 3422
Size: 32 2918 32 3430
Fragments arranged by size:
3430 2918 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2483 1316
Size: 1167 5245
XbaI T'CTAG_A
Cuts at: 3748 3748
Size: 6412
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6412
XmaI C'CCGG_G
Cuts at: 1870 2243 2549 3737 1870
Size: 373 306 1188 4545
Fragments arranged by size:
4545 1188 373 306
XmnI GAAnn'nnTTC
Cuts at: 2238 3555 4541 2238
Size: 1317 986 4109
Fragments arranged by size:
4109 1317 986
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3641 4220 276
Size: 53 83 186 936 2107 579 2468
Fragments arranged by size:
2468 2107 936 579 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I
EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI