Restriction map for
pFN21AA1998
(Circular) (Six-base) MAPSORT of: pFN21AA1998.seq Check: 1461 from: 1 to: 9820
pFN21AA1998 9820 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6363 6363
Size: 9820
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2658 5760 7630 278
Size: 53 83 186 936 1122 3102 1870 2468
Fragments arranged by size:
3102 2468 1870 1122 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3123 6834 7163 1937
Size: 1186 3711 329 4594
Fragments arranged by size:
4594 3711 1186 329
Acc65I G'GTAC_C
Cuts at: 4286 7141 4286
Size: 2855 6965
AclI AA'CG_TT
Cuts at: 1563 2163 7947 8320 1563
Size: 600 5784 373 3063
Fragments arranged by size:
5784 3063 600 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2106 2180 2928 3342 4288 4612 5598 5649
Size: 474 74 748 414 946 324 986 51
Cuts at: 5649 6146 6317 7883 9267 9535 1632
Size: 497 171 1566 1384 268 1917
Fragments arranged by size:
1917 1566 1384 986 946 748 497 474
414 324 268 171 74 51
AfeI AGC'GCT
Cuts at: 5094 5094
Size: 9820
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9623
AflIII A'CryG_T
Cuts at: 1203 2131 5221 8719 9696 1203
Size: 928 3090 3498 977 1327
Fragments arranged by size:
3498 3090 1327 977 928
AgeI A'CCGG_T
Cuts at: 8651 8651
Size: 9820
AhdI GACnn_n'nnGTC
Cuts at: 4559 6280 8549 4559
Size: 1721 2269 5830
Fragments arranged by size:
5830 2269 1721
AleI CACnn'nnGTG
Cuts at: 1166 4042 1166
Size: 2876 6944
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5739 1184
Size: 4555 5265
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3520 3552 3520
Size: 32 9788
AlwNI CAG_nnn'CTG
Cuts at: 2356 2906 3845 4606 5242 6541 6583 6893 7563
Size: 550 939 761 636 1299 42 310 670
Cuts at: 7563 9135 2356
Size: 1572 3041
Fragments arranged by size:
3041 1572 1299 939 761 670 636 550
310 42
ApaI G_GGCC'C
Cuts at: 6574 6574
Size: 9820
ApaLI G'TGCA_C
Cuts at: 4067 6323 7877 9033 4067
Size: 2256 1554 1156 4854
Fragments arranged by size:
4854 2256 1554 1156
ApoI r'AATT_y
Cuts at: 1493 2662 2923 3693 3795 4109 4156 4680 4777
Size: 1169 261 770 102 314 47 524 97
Cuts at: 4777 4862 4930 4977 5505 5646 5688 6091 6136
Size: 85 68 47 528 141 42 403 45
Cuts at: 6136 6997 7129 7355 7507 9616 1493
Size: 861 132 226 152 2109 1697
Fragments arranged by size:
2109 1697 1169 861 770 528 524 403
314 261 226 152 141 132 102 97
85 68 47 47 45 42
AseI AT'TA_AT
Cuts at: 160 1032 3177 8374 160
Size: 872 2145 5197 1606
Fragments arranged by size:
5197 2145 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9820
AvaI C'yCGr_G
Cuts at: 1870 1943 2560 2694 6026 6647 7145 1870
Size: 73 617 134 3332 621 498 4545
Fragments arranged by size:
4545 3332 621 617 498 134 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4802 4835 4802
Size: 33 9787
BamHI G'GATC_C
Cuts at: 4510 5550 6022 6030 7150 7517 4510
Size: 1040 472 8 1120 367 6813
Fragments arranged by size:
6813 1120 1040 472 367 8
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2600 4286 5885 7141 8596
Size: 325 217 627 813 1686 1599 1256 1455
Cuts at: 8596 618
Size: 1842
Fragments arranged by size:
1842 1686 1599 1455 1256 813 627 325
217
BanII G_rGCy'C
Cuts at: 1391 2261 2412 3521 4397 5772 6574 7139 1391
Size: 870 151 1109 876 1375 802 565 4072
Fragments arranged by size:
4072 1375 1109 876 870 802 565 151
BbeI G_GCGC'C
Cuts at: 5889 5889
Size: 9820
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2658 3012 5619 928
Size: 971 759 354 2607 5129
Fragments arranged by size:
5129 2607 971 759 354
BbvCI CC'TCA_GC
Cuts at: 4460 6524 4460
Size: 2064 7756
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2309 2343 2582 2616 8011 8045 1903
Size: 34 372 34 239 34 5395 34 3678
Fragments arranged by size:
5395 3678 372 239 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7712 8922 7712
Size: 1210 8610
BclI T'GATC_A
Cuts at: 1548 3145 5392 5469 1548
Size: 1597 2247 77 5899
Fragments arranged by size:
5899 2247 1597 77
BfrBI ATG'CAT
Cuts at: 4591 4989 4591
Size: 398 9422
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2378 5639 6588
Size: 107 122 71 631 605 706 3261 949
Cuts at: 6588 8431 136
Size: 1843 1525
Fragments arranged by size:
3261 1843 1525 949 706 631 605 122
107 71
BglII A'GATC_T
Cuts at: 4623 9815 4623
Size: 5192 4628
BlpI GC'TnA_GC
Cuts at: 7237 7237
Size: 9820
Bme1580I G_kGCm'C
Cuts at: 1790 4071 6327 6574 7881 9037 1790
Size: 2281 2256 247 1307 1156 2573
Fragments arranged by size:
2573 2281 2256 1307 1156 247
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9820
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2536 3270 3540
Size: 416 53 83 399 24 1119 734 270
Cuts at: 3540 7551 8509 442
Size: 4011 958 1753
Fragments arranged by size:
4011 1753 1119 958 734 416 399 270
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9820
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2720 2720
Size: 9820
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2153 2875 3489 3580 3675 4892 6200 6329
Size: 914 722 614 91 95 1217 1308 129
Cuts at: 6329 6473 6479 6536 7107 8480 1239
Size: 144 6 57 571 1373 2579
Fragments arranged by size:
2579 1373 1308 1217 914 722 614 571
144 129 95 91 57 6
Bpu10I CC'TnA_GC
Cuts at: 4460 6524 4460
Size: 2064 7756
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3508 5189 6824 7945 8810 9108 3508
Size: 1681 1635 1121 865 298 4220
Fragments arranged by size:
4220 1681 1635 1121 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3516 4193 4368 8483 882
Size: 617 2017 677 175 4115 2219
Fragments arranged by size:
4115 2219 2017 677 617 175
BsaAI yAC'GTr
Cuts at: 493 1204 2278 2627 7611 493
Size: 711 1074 349 4984 2702
Fragments arranged by size:
4984 2702 1074 711 349
BsaBI GATnn'nnATC
Cuts at: 2048 3027 6021 9814 2048
Size: 979 2994 3793 2054
Fragments arranged by size:
3793 2994 2054 979
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2655 3453 4969 5757
Size: 53 83 186 936 1122 798 1516 788
Cuts at: 5757 5886 7627 8009 8644 275
Size: 129 1741 382 635 1451
Fragments arranged by size:
1741 1516 1451 1122 936 798 788 635
382 186 129 83 53
BsaWI w'CCGG_w
Cuts at: 2245 2808 3580 8253 8651 8925 9072 9483 2245
Size: 563 772 4673 398 274 147 411 2582
Fragments arranged by size:
4673 2582 772 563 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5910 5940 6218 6248 6422
Size: 30 165 30 4507 30 278 30 174
Cuts at: 6422 6428 6452 6458 1178
Size: 6 24 6 4540
Fragments arranged by size:
4540 4507 278 174 165 30 30 30
30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 3511 4661 5465 5720 6323 6467 6628
Size: 1234 1091 1150 804 255 603 144 161
Cuts at: 6628 1186
Size: 4378
Fragments arranged by size:
4378 1234 1150 1091 804 603 255 161
144
BseYI C'CCAG_C
Cuts at: 3327 6573 6586 9023 9519 3327
Size: 3246 13 2437 496 3628
Fragments arranged by size:
3628 3246 2437 496 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2720 2919 1163
Size: 573 984 199 8064
Fragments arranged by size:
8064 984 573 199
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4792 6607 7270 8031 9059 1540
Size: 275 186 2791 1815 663 761 1028 2301
Fragments arranged by size:
2791 2301 1815 1028 761 663 275 186
BsiHKAI G_wGCw'C
Cuts at: 2389 2412 3521 4071 6327 6633 7881 7966 9037
Size: 23 1109 550 2256 306 1248 85 1071
Cuts at: 9037 2389
Size: 3172
Fragments arranged by size:
3172 2256 1248 1109 1071 550 306 85
23
BsmI GAATG_Cn'
Cuts at: 3475 4989 4991 7331 7424 3475
Size: 1514 2 2340 93 5871
Fragments arranged by size:
5871 2340 1514 93 2
BsmBI CGTCTCn'nnnn_
Cuts at: 2195 5539 6064 6152 2195
Size: 3344 525 88 5863
Fragments arranged by size:
5863 3344 525 88
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2261 2389 2412 3521 4071 4397 5772
Size: 399 471 128 23 1109 550 326 1375
Cuts at: 5772 6327 6574 6633 7139 7881 7966 9037 1391
Size: 555 247 59 506 742 85 1071 2174
Fragments arranged by size:
2174 1375 1109 1071 742 555 550 506
471 399 326 247 128 85 59 23
BspEI T'CCGG_A
Cuts at: 2808 9483 2808
Size: 6675 3145
BspHI T'CATG_A
Cuts at: 2136 3882 5950 6685 7709 9439 2136
Size: 1746 2068 735 1024 1730 2517
Fragments arranged by size:
2517 2068 1746 1730 1024 735
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3838 4350 6363 7175 844
Size: 2994 512 2013 812 3489
Fragments arranged by size:
3489 2994 2013 812 512
BsrBI CCG'CTC
Cuts at: 1394 1823 5745 6427 6433 6520 7707 1394
Size: 429 3922 682 6 87 1187 3507
Fragments arranged by size:
3922 3507 1187 682 429 87 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 3071 5660 8315 8489 65
Size: 1183 1823 2589 2655 174 1396
Fragments arranged by size:
2655 2589 1823 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3087 6606 8464 8651 1700
Size: 110 1277 3519 1858 187 2869
Fragments arranged by size:
3519 2869 1858 1277 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9820
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9820
BssSI C'ACGA_G
Cuts at: 2411 7880 8892 2411
Size: 5469 1012 3339
Fragments arranged by size:
5469 3339 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2393 6583 794
Size: 1599 4190 4031
Fragments arranged by size:
4190 4031 1599
BstBI TT'CG_AA
Cuts at: 6863 8624 6863
Size: 1761 8059
BstEII G'GTnAC_C
Cuts at: 1184 2248 6043 1184
Size: 1064 3795 4961
Fragments arranged by size:
4961 3795 1064
BstXI CCAn_nnnn'nTGG
Cuts at: 2647 4324 2647
Size: 1677 8143
BstYI r'GATC_y
Cuts at: 1972 2117 4510 4623 5172 5550 6022 6030 6198
Size: 145 2393 113 549 378 472 8 168
Cuts at: 6198 7150 7517 7902 7919 9371 9457 9469 9815
Size: 952 367 385 17 1452 86 12 346
Cuts at: 9815 1972
Size: 1977
Fragments arranged by size:
2393 1977 1452 952 549 472 385 378
367 346 168 145 113 86 17 12
8
BtgI C'CryG_G
Cuts at: 513 1065 2004 2188 2253 4389 4671 6624 513
Size: 552 939 184 65 2136 282 1953 3709
Fragments arranged by size:
3709 2136 1953 939 552 282 184 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9081
Fragments arranged by size:
9081 683 56
BtsI GCAGTG_nn'
Cuts at: 2387 3192 3644 4004 4037 4049 4471 5871 7336
Size: 805 452 360 33 12 422 1400 1465
Cuts at: 7336 8130 8150 2387
Size: 794 20 4057
Fragments arranged by size:
4057 1465 1400 805 794 452 422 360
33 20 12
ClaI AT'CG_AT
Cuts at: 1554 6667 1554
Size: 5113 4707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9785
DraI TTT'AAA
Cuts at: 4900 5133 7124 4900
Size: 233 1991 7596
Fragments arranged by size:
7596 1991 233
DraIII CAC_nnn'GTG
Cuts at: 3231 3293 3231
Size: 62 9758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6056 8827 809
Size: 507 4740 2771 1802
Fragments arranged by size:
4740 2771 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5640 6054 6241
Size: 54 1425 50 275 18 3810 414 187
Cuts at: 6241 6315 6604 6901 7267 8156 9785 8
Size: 74 289 297 366 889 1629 43
Fragments arranged by size:
3810 1629 1425 889 414 366 297 289
275 187 74 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6604 7267 1537
Size: 275 4792 663 4090
Fragments arranged by size:
4792 4090 663 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2505 2589 2810 2907 3744 4402 5998 7750
Size: 1152 84 221 97 837 658 1596 1752
Cuts at: 7750 1353
Size: 3423
Fragments arranged by size:
3423 1752 1596 1152 837 658 221 97
84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5948 8407 8781 8927 387
Size: 1064 138 4359 2459 374 146 1280
Fragments arranged by size:
4359 2459 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2410 3519 2410
Size: 1109 8711
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2106 2153 2180 2875 2928 3342 3489
Size: 393 474 47 27 695 53 414 147
Cuts at: 3489 3580 3675 4288 4612 4892 5598 5649 6146
Size: 91 95 613 324 280 706 51 497
Cuts at: 6146 6200 6317 6329 6473 6479 6536 7107 7883
Size: 54 117 12 144 6 57 571 776
Cuts at: 7883 8480 9267 9535 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 776 706 695 613 597 571
497 474 414 393 324 280 268 147
144 117 95 91 57 54 53 51
47 27 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 5793 5793
Size: 9820
EcoO109I rG'GnC_Cy
Cuts at: 2524 6229 2524
Size: 3705 6115
EcoRI G'AATT_C
Cuts at: 2923 3795 4862 4977 7129 7507 2923
Size: 872 1067 115 2152 378 5236
Fragments arranged by size:
5236 2152 1067 872 378 115
EcoRV GAT'ATC
Cuts at: 3043 3166 5317 3043
Size: 123 2151 7546
Fragments arranged by size:
7546 2151 123
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2048 2048
Size: 9820
FspI TGC'GCA
Cuts at: 6371 8326 6371
Size: 1955 7865
HaeII r_GCGC'y
Cuts at: 1678 5096 5889 6364 6541 8967 1678
Size: 3418 793 475 177 2426 2531
Fragments arranged by size:
3418 2531 2426 793 475 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3886 3918 4545 4577 5910
Size: 32 135 32 2314 32 627 32 1333
Cuts at: 5910 5942 6218 6250 6266 6298 6428 6460 6667
Size: 32 276 32 16 32 130 32 207
Cuts at: 6667 6699 8461 8493 8535 8567 1373
Size: 32 1762 32 42 32 2626
Fragments arranged by size:
2626 2314 1762 1333 627 276 207 135
130 42 32 32 32 32 32 32
32 32 32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 4891 7164 7410 669
Size: 988 281 2953 2273 246 3079
Fragments arranged by size:
3079 2953 2273 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2686 3759 4300 5423 6711 748
Size: 1938 1073 541 1123 1288 3857
Fragments arranged by size:
3857 1938 1288 1123 1073 541
HpaI GTT'AAC
Cuts at: 4891 7410 4891
Size: 2519 7301
KasI G'GCGC_C
Cuts at: 5885 5885
Size: 9820
KpnI G_GTAC'C
Cuts at: 4290 7145 4290
Size: 2855 6965
MfeI C'AATT_G
Cuts at: 7419 7419
Size: 9820
MluI A'CGCG_T
Cuts at: 9696 9696
Size: 9820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2766 3390 4201 8934 9118 1305
Size: 1461 624 811 4733 184 2007
Fragments arranged by size:
4733 2007 1461 811 624 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5642 6317 10
Size: 54 1425 343 3810 675 3513
Fragments arranged by size:
3810 3513 1425 675 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4042 4371 5721 6771 7778 8137 8296
Size: 648 2876 329 1350 1050 1007 359 159
Cuts at: 8296 518
Size: 2042
Fragments arranged by size:
2876 2042 1350 1050 1007 648 359 329
159
MspA1I CmG'CkG
Cuts at: 1606 2895 4350 4657 6358 6391 6626 6893 7236
Size: 1289 1455 307 1701 33 235 267 343
Cuts at: 7236 7913 9061 9306 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1701 1455 1289 1148 677 343 307
267 245 235 33
NaeI GCC'GGC
Cuts at: 3089 6608 3089
Size: 3519 6301
NarI GG'CG_CC
Cuts at: 5886 5886
Size: 9820
NcoI C'CATG_G
Cuts at: 513 1065 2004 2188 2253 4389 513
Size: 552 939 184 65 2136 5944
Fragments arranged by size:
5944 2136 939 552 184 65
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9820
NgoMIV G'CCGG_C
Cuts at: 3087 6606 3087
Size: 3519 6301
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9820
NotI GC'GGCC_GC
Cuts at: 7267 7267
Size: 9820
NruI TCG'CGA
Cuts at: 6288 6288
Size: 9820
NsiI A_TGCA'T
Cuts at: 4593 4991 4593
Size: 398 9422
NspI r_CATG'y
Cuts at: 1132 1229 2135 3103 4101 5225 7178 8723 1132
Size: 97 906 968 998 1124 1953 1545 2229
Fragments arranged by size:
2229 1953 1545 1124 998 968 906 97
PacI TTA_AT'TAA
Cuts at: 5106 5106
Size: 9820
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9820
PciI A'CATG_T
Cuts at: 2131 5221 8719 2131
Size: 3090 3498 3232
Fragments arranged by size:
3498 3232 3090
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2189 2578 4184 6040 6208 6541 1108
Size: 1081 389 1606 1856 168 333 4387
Fragments arranged by size:
4387 1856 1606 1081 389 333 168
PfoI T'CCnGG_A
Cuts at: 6495 6495
Size: 9820
PmeI GTTT'AAAC
Cuts at: 7124 7124
Size: 9820
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7889 7921 9402 9434 7889
Size: 32 1481 32 8275
Fragments arranged by size:
8275 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9820
PsiI TTA'TAA
Cuts at: 7028 7390 7028
Size: 362 9458
PspOMI G'GGCC_C
Cuts at: 6570 6570
Size: 9820
PstI C_TGCA'G
Cuts at: 830 2021 2345 3085 3999 4044 4355 4656 6423
Size: 1191 324 740 914 45 311 301 1767
Cuts at: 6423 7020 7172 830
Size: 597 152 3478
Fragments arranged by size:
3478 1767 1191 914 740 597 324 311
301 152 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9820
PvuII CAG'CTG
Cuts at: 4350 4657 4350
Size: 307 9513
SacI G_AGCT'C
Cuts at: 2412 3521 2412
Size: 1109 8711
SacII CC_GC'GG
Cuts at: 6627 6627
Size: 9820
SalI G'TCGA_C
Cuts at: 1936 7162 1936
Size: 5226 4594
SapI GCTCTTCn'nnn_
Cuts at: 3744 4402 3744
Size: 658 9162
SbfI CC_TGCA'GG
Cuts at: 7172 7172
Size: 9820
ScaI AGT'ACT
Cuts at: 8068 9780 8068
Size: 1712 8108
SfcI C'TryA_G
Cuts at: 826 1045 2017 2341 3081 3261 3629 3995 4040
Size: 219 972 324 740 180 368 366 45
Cuts at: 4040 4351 4382 4407 4652 4729 5298 5562 6419
Size: 311 31 25 245 77 569 264 857
Cuts at: 6419 7016 7168 8303 8984 9175 826
Size: 597 152 1135 681 191 1471
Fragments arranged by size:
1471 1135 972 857 740 681 597 569
368 366 324 311 264 245 219 191
180 152 77 45 31 25
SfoI GGC'GCC
Cuts at: 5887 5887
Size: 9820
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9820
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9820
SmaI CCC'GGG
Cuts at: 1872 2562 6028 7147 1872
Size: 690 3466 1119 4545
Fragments arranged by size:
4545 3466 1119 690
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3523 5168 6839 7924 8825 9087
Size: 197 926 1580 1645 1671 1085 901 262
Cuts at: 9087 820
Size: 1553
Fragments arranged by size:
1671 1645 1580 1553 1085 926 901 262
197
SnaBI TAC'GTA
Cuts at: 493 7611 493
Size: 7118 2702
SpeI A'CTAG_T
Cuts at: 152 6818 152
Size: 6666 3154
SphI G_CATG'C
Cuts at: 1132 4101 7178 1132
Size: 2969 3077 3774
Fragments arranged by size:
3774 3077 2969
SspI AAT'ATT
Cuts at: 5 52 7108 7744 5
Size: 47 7056 636 2081
Fragments arranged by size:
7056 2081 636 47
StuI AGG'CCT
Cuts at: 1517 2576 2846 5873 1517
Size: 1059 270 3027 5464
Fragments arranged by size:
5464 3027 1059 270
StyI C'CwwG_G
Cuts at: 513 1065 2004 2188 2253 2397 2577 3447 4150
Size: 552 939 184 65 144 180 870 703
Cuts at: 4150 4293 4323 4389 5520 7259 513
Size: 143 30 66 1131 1739 3074
Fragments arranged by size:
3074 1739 1131 939 870 703 552 184
180 144 143 66 65 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3703 4806 7847 7864 8017 8202 8647
Size: 1255 2123 1103 3041 17 153 185 445
Cuts at: 8647 325
Size: 1498
Fragments arranged by size:
3041 2123 1498 1255 1103 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5049 6718 8066
Size: 274 80 33 51 1443 3072 1669 1348
Cuts at: 8066 9778 96
Size: 1712 138
Fragments arranged by size:
3072 1712 1669 1443 1348 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3526 3558 4131 4163 4190 4222 5501 5533 6022
Size: 32 573 32 27 32 1279 32 489
Cuts at: 6022 6054 6422 6454 6959 6991 9780 9812 3526
Size: 32 368 32 505 32 2789 32 3534
Fragments arranged by size:
3534 2789 1279 573 505 489 368 32
32 32 32 32 32 32 32 27
Tth111I GACn'n_nGTC
Cuts at: 1316 5924 1316
Size: 4608 5212
XbaI T'CTAG_A
Cuts at: 2443 5764 7156 2443
Size: 3321 1392 5107
Fragments arranged by size:
5107 3321 1392
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9820
XmaI C'CCGG_G
Cuts at: 1870 2560 6026 7145 1870
Size: 690 3466 1119 4545
Fragments arranged by size:
4545 3466 1119 690
XmnI GAAnn'nnTTC
Cuts at: 2467 2499 3609 4159 4989 7949 2467
Size: 32 1110 550 830 2960 4338
Fragments arranged by size:
4338 2960 1110 830 550 32
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2656 5758 7628 276
Size: 53 83 186 936 1122 3102 1870 2468
Fragments arranged by size:
3102 2468 1870 1122 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BsiWI BstZ17I Bsu36I FseI FspAI PmlI
PpuMI PspXI PsrI RsrII SanDI SexAI SfiI SrfI
SwaI XcmI