Restriction map for
pFN21AA1975
(Circular) (Six-base) MAPSORT of: pFN21AA1975.seq Check: 5086 from: 1 to: 6355
pFN21AA1975 6355 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3375 3375
Size: 6355
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3546 4165 278
Size: 53 83 186 936 2010 619 2468
Fragments arranged by size:
2468 2010 936 619 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3698 1937
Size: 1761 4594
Acc65I G'GTAC_C
Cuts at: 3676 3676
Size: 6355
AclI AA'CG_TT
Cuts at: 1563 4482 4855 1563
Size: 2919 373 3063
Fragments arranged by size:
3063 2919 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2432 2662 4418 5802 6070 1632
Size: 800 230 1756 1384 268 1917
Fragments arranged by size:
1917 1756 1384 800 268 230
AfeI AGC'GCT
Cuts at: 3576 3576
Size: 6355
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6158
AflIII A'CryG_T
Cuts at: 1203 5254 6231 1203
Size: 4051 977 1327
Fragments arranged by size:
4051 1327 977
AgeI A'CCGG_T
Cuts at: 5186 5186
Size: 6355
AhdI GACnn_n'nnGTC
Cuts at: 5084 5084
Size: 6355
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6355
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3635 1184
Size: 2451 3904
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3279 3311 3279
Size: 32 6323
AlwNI CAG_nnn'CTG
Cuts at: 2923 3225 3367 3523 4098 5670 2923
Size: 302 142 156 575 1572 3608
Fragments arranged by size:
3608 1572 575 302 156 142
ApaLI G'TGCA_C
Cuts at: 2117 4412 5568 2117
Size: 2295 1156 2904
Fragments arranged by size:
2904 2295 1156
ApoI r'AATT_y
Cuts at: 1493 3664 3890 4042 6151 1493
Size: 2171 226 152 2109 1697
Fragments arranged by size:
2171 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4909 160
Size: 872 3877 1606
Fragments arranged by size:
3877 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
AvaI C'yCGr_G
Cuts at: 1870 1943 3552 3680 1870
Size: 73 1609 128 4545
Fragments arranged by size:
4545 1609 128 73
BamHI G'GATC_C
Cuts at: 3294 3685 4052 3294
Size: 391 367 5597
Fragments arranged by size:
5597 391 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3125 3676 5131 618
Size: 325 217 627 1338 551 1455 1842
Fragments arranged by size:
1842 1455 1338 627 551 325 217
BanII G_rGCy'C
Cuts at: 1391 3180 3674 1391
Size: 1789 494 4072
Fragments arranged by size:
4072 1789 494
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2420 928
Size: 971 521 4863
Fragments arranged by size:
4863 971 521
BbvCI CC'TCA_GC
Cuts at: 2808 2808
Size: 6355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4546 4580 1903
Size: 34 2609 34 3678
Fragments arranged by size:
3678 2609 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4247 5457 4247
Size: 1210 5145
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6355
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2570 3593 4966
Size: 107 122 71 631 605 898 1023 1373
Cuts at: 4966 136
Size: 1525
Fragments arranged by size:
1525 1373 1023 898 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2066 6350 2066
Size: 4284 2071
BlpI GC'TnA_GC
Cuts at: 3772 3772
Size: 6355
Bme1580I G_kGCm'C
Cuts at: 1790 2121 4416 5572 1790
Size: 331 2295 1156 2573
Fragments arranged by size:
2573 2295 1156 331
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6355
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2679 4086 5044
Size: 416 53 83 399 24 1262 1407 958
Cuts at: 5044 442
Size: 1753
Fragments arranged by size:
1753 1407 1262 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2348 2572 2677 3173 3583 5015 1239
Size: 1109 224 105 496 410 1432 2579
Fragments arranged by size:
2579 1432 1109 496 410 224 105
Bpu10I CC'TnA_GC
Cuts at: 2476 2808 3179 2476
Size: 332 371 5652
Fragments arranged by size:
5652 371 332
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4480 5345 5643 4480
Size: 865 298 5192
Fragments arranged by size:
5192 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3038 3440 5018 882
Size: 617 1539 402 1578 2219
Fragments arranged by size:
2219 1578 1539 617 402
BsaAI yAC'GTr
Cuts at: 493 1204 4146 493
Size: 711 2942 2702
Fragments arranged by size:
2942 2702 711
BsaBI GATnn'nnATC
Cuts at: 6349 6349
Size: 6355
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3543 4162 4544 5179
Size: 53 83 186 936 2010 619 382 635
Cuts at: 5179 275
Size: 1451
Fragments arranged by size:
2010 1451 936 635 619 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4788 5186 5460 5607 6018 4788
Size: 398 274 147 411 5125
Fragments arranged by size:
5125 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2150 2180 1178
Size: 30 165 30 747 30 5353
Fragments arranged by size:
5353 747 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2158 2897 3449 1186
Size: 972 739 552 4092
Fragments arranged by size:
4092 972 739 552
BseYI C'CCAG_C
Cuts at: 2186 2659 2964 5558 6054 2186
Size: 473 305 2594 496 2487
Fragments arranged by size:
2594 2487 496 473 305
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3325 3454 1163
Size: 573 1589 129 4064
Fragments arranged by size:
4064 1589 573 129
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3805 4566 5594 1540
Size: 275 186 1804 761 1028 2301
Fragments arranged by size:
2301 1804 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2121 2460 3180 4416 4501 5572 2121
Size: 339 720 1236 85 1071 2904
Fragments arranged by size:
2904 1236 1071 720 339 85
BsmI GAATG_Cn'
Cuts at: 3866 3959 3866
Size: 93 6262
BsmBI CGTCTCn'nnnn_
Cuts at: 3456 3456
Size: 6355
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2460 3180 3674 4416 4501 5572
Size: 399 331 339 720 494 742 85 1071
Cuts at: 5572 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 720 494 399 339 331
85
BspEI T'CCGG_A
Cuts at: 6018 6018
Size: 6355
BspHI T'CATG_A
Cuts at: 4244 5974 4244
Size: 1730 4625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2727 3375 3402 3710 844
Size: 1883 648 27 308 3489
Fragments arranged by size:
3489 1883 648 308 27
BsrBI CCG'CTC
Cuts at: 1394 1823 2355 2887 4242 1394
Size: 429 532 532 1355 3507
Fragments arranged by size:
3507 1355 532 532 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2608 3211 4850 5024 65
Size: 1183 1360 603 1639 174 1396
Fragments arranged by size:
1639 1396 1360 1183 603 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4999 5186 1700
Size: 110 3189 187 2869
Fragments arranged by size:
3189 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6355
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6355
BssSI C'ACGA_G
Cuts at: 4415 5427 4415
Size: 1012 5343
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3370 794
Size: 2576 3779
BstBI TT'CG_AA
Cuts at: 5159 5159
Size: 6355
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6355
BstXI CCAn_nnnn'nTGG
Cuts at: 2550 2620 2550
Size: 70 6285
BstYI r'GATC_y
Cuts at: 1972 2066 2919 3294 3685 4052 4437 4454 5906
Size: 94 853 375 391 367 385 17 1452
Cuts at: 5906 5992 6004 6350 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 853 391 385 375 367 346
94 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2262 2988 3562 513
Size: 552 1197 726 574 3306
Fragments arranged by size:
3306 1197 726 574 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5616
Fragments arranged by size:
5616 683 56
BtsI GCAGTG_nn'
Cuts at: 2226 2802 3028 3871 4665 4685 2226
Size: 576 226 843 794 20 3896
Fragments arranged by size:
3896 843 794 576 226 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6355
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6320
DraI TTT'AAA
Cuts at: 2154 2866 3659 2154
Size: 712 793 4850
Fragments arranged by size:
4850 793 712
DraIII CAC_nnn'GTG
Cuts at: 2442 3034 2442
Size: 592 5763
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5362 809
Size: 507 4046 1802
Fragments arranged by size:
4046 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2552 2848 2925
Size: 54 1425 50 275 18 722 296 77
Cuts at: 2925 3167 3802 4691 6320 8
Size: 242 635 889 1629 43
Fragments arranged by size:
1629 1425 889 722 635 296 275 242
77 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3802 1537
Size: 275 1990 4090
Fragments arranged by size:
4090 1990 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3273 4285 1353
Size: 1920 1012 3423
Fragments arranged by size:
3423 1920 1012
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4942 5316 5462 387
Size: 1064 138 3353 374 146 1280
Fragments arranged by size:
3353 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3178 3178
Size: 6355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2348 2432 2572 2662 2677 3173 3583
Size: 393 716 84 140 90 15 496 410
Cuts at: 3583 4418 5015 5802 6070 1239
Size: 835 597 787 268 1524
Fragments arranged by size:
1524 835 787 716 597 496 410 393
268 140 90 84 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2612 2612
Size: 6355
EcoO109I rG'GnC_Cy
Cuts at: 2203 3391 2203
Size: 1188 5167
EcoRI G'AATT_C
Cuts at: 3664 4042 3664
Size: 378 5977
EcoRV GAT'ATC
Cuts at: 2653 2653
Size: 6355
FspI TGC'GCA
Cuts at: 2578 4861 2578
Size: 2283 4072
HaeII r_GCGC'y
Cuts at: 1678 3479 3578 5502 1678
Size: 1801 99 1924 2531
Fragments arranged by size:
2531 1924 1801 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2023 2055 2780 2812 3037
Size: 32 135 32 451 32 725 32 225
Cuts at: 3037 3069 4996 5028 5070 5102 1373
Size: 32 1927 32 42 32 2626
Fragments arranged by size:
2626 1927 725 451 225 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3270 3699 3945 669
Size: 988 281 1332 429 246 3079
Fragments arranged by size:
3079 1332 988 429 281 246
HindIII A'AGCT_T
Cuts at: 748 2473 748
Size: 1725 4630
HpaI GTT'AAC
Cuts at: 3945 3945
Size: 6355
KpnI G_GTAC'C
Cuts at: 3680 3680
Size: 6355
MfeI C'AATT_G
Cuts at: 3954 3954
Size: 6355
MluI A'CGCG_T
Cuts at: 6231 6231
Size: 6355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2298 5469 5653 1305
Size: 993 3171 184 2007
Fragments arranged by size:
3171 2007 993 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2554 2850 2927 3169 10
Size: 54 1425 343 722 296 77 242 3196
Fragments arranged by size:
3196 1425 722 343 296 242 77 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3617 4313 4672 4831 518
Size: 648 2451 696 359 159 2042
Fragments arranged by size:
2451 2042 696 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2968 3383 3771 4448 5596 5841 1606
Size: 1362 415 388 677 1148 245 2120
Fragments arranged by size:
2120 1362 1148 677 415 388 245
NcoI C'CATG_G
Cuts at: 513 1065 2262 2988 513
Size: 552 1197 726 3880
Fragments arranged by size:
3880 1197 726 552
NdeI CA'TA_TG
Cuts at: 387 2634 2672 387
Size: 2247 38 4070
Fragments arranged by size:
4070 2247 38
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6355
NotI GC'GGCC_GC
Cuts at: 3802 3802
Size: 6355
NspI r_CATG'y
Cuts at: 1132 1229 2386 3016 3713 5258 1132
Size: 97 1157 630 697 1545 2229
Fragments arranged by size:
2229 1545 1157 697 630 97
PasI CC'CwG_GG
Cuts at: 1348 3355 1348
Size: 2007 4348
PciI A'CATG_T
Cuts at: 5254 5254
Size: 6355
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2268 2923 3034 1108
Size: 1160 655 111 4429
Fragments arranged by size:
4429 1160 655 111
PmeI GTTT'AAAC
Cuts at: 3659 3659
Size: 6355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4424 4456 5937 5969 4424
Size: 32 1481 32 4810
Fragments arranged by size:
4810 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2203 3391 2203
Size: 1188 5167
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6355
PsiI TTA'TAA
Cuts at: 3925 3925
Size: 6355
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3085 3117 3085
Size: 32 6323
PstI C_TGCA'G
Cuts at: 830 2937 3000 3630 3707 830
Size: 2107 63 630 77 3478
Fragments arranged by size:
3478 2107 630 77 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6355
PvuII CAG'CTG
Cuts at: 2968 2968
Size: 6355
SacI G_AGCT'C
Cuts at: 3180 3180
Size: 6355
SalI G'TCGA_C
Cuts at: 1936 3697 1936
Size: 1761 4594
SbfI CC_TGCA'GG
Cuts at: 3707 3707
Size: 6355
ScaI AGT'ACT
Cuts at: 4603 6315 4603
Size: 1712 4643
SfcI C'TryA_G
Cuts at: 826 1045 2933 2996 3626 3703 4838 5519 5710
Size: 219 1888 63 630 77 1135 681 191
Cuts at: 5710 826
Size: 1471
Fragments arranged by size:
1888 1471 1135 681 630 219 191 77
63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6355
SmaI CCC'GGG
Cuts at: 1872 3682 1872
Size: 1810 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4459 5360 5622 820
Size: 197 926 2516 901 262 1553
Fragments arranged by size:
2516 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4146 493
Size: 3653 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6355
SphI G_CATG'C
Cuts at: 1132 2386 3713 1132
Size: 1254 1327 3774
Fragments arranged by size:
3774 1327 1254
SspI AAT'ATT
Cuts at: 5 52 2497 4279 5
Size: 47 2445 1782 2081
Fragments arranged by size:
2445 2081 1782 47
StuI AGG'CCT
Cuts at: 1517 3596 1517
Size: 2079 4276
StyI C'CwwG_G
Cuts at: 513 1065 2025 2262 2613 2988 3794 513
Size: 552 960 237 351 375 806 3074
Fragments arranged by size:
3074 960 806 552 375 351 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2254 2596 2651 4382 4399 4552 4737
Size: 1255 674 342 55 1731 17 153 185
Cuts at: 4737 5182 325
Size: 445 1498
Fragments arranged by size:
1731 1498 1255 674 445 342 185 153
55 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4601 6313 96
Size: 274 80 33 51 1443 2624 1712 138
Fragments arranged by size:
2624 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6315 6347 6315
Size: 32 6323
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6355
XbaI T'CTAG_A
Cuts at: 3691 3691
Size: 6355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3014 3073 3119 3412 3014
Size: 59 46 293 5957
Fragments arranged by size:
5957 293 59 46
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6355
XmaI C'CCGG_G
Cuts at: 1870 3680 1870
Size: 1810 4545
XmnI GAAnn'nnTTC
Cuts at: 4484 4484
Size: 6355
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3544 4163 276
Size: 53 83 186 936 2010 619 2468
Fragments arranged by size:
2468 2010 936 619 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I Bsu36I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI