Restriction map for
pFN21AA1973
(Circular) (Six-base) MAPSORT of: pFN21AA1973.seq Check: 1019 from: 1 to: 8050
pFN21AA1973 8050 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5860 278
Size: 53 83 186 936 4324 2468
Fragments arranged by size:
4324 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4869 5393 1937
Size: 2932 524 4594
Fragments arranged by size:
4594 2932 524
Acc65I G'GTAC_C
Cuts at: 5371 5371
Size: 8050
AclI AA'CG_TT
Cuts at: 1563 6177 6550 1563
Size: 4614 373 3063
Fragments arranged by size:
4614 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3273 4140 4431 4520 6113 7497 7765 1632
Size: 1641 867 291 89 1593 1384 268 1917
Fragments arranged by size:
1917 1641 1593 1384 867 291 268 89
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7853
AflIII A'CryG_T
Cuts at: 1203 4999 6949 7926 1203
Size: 3796 1950 977 1327
Fragments arranged by size:
3796 1950 1327 977
AgeI A'CCGG_T
Cuts at: 6881 6881
Size: 8050
AhdI GACnn_n'nnGTC
Cuts at: 3108 6779 3108
Size: 3671 4379
AleI CACnn'nnGTG
Cuts at: 1166 2978 1166
Size: 1812 6238
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3767 4083 1184
Size: 2583 316 5151
Fragments arranged by size:
5151 2583 316
AlwNI CAG_nnn'CTG
Cuts at: 2043 5281 5793 7365 2043
Size: 3238 512 1572 2728
Fragments arranged by size:
3238 2728 1572 512
ApaLI G'TGCA_C
Cuts at: 2162 6107 7263 2162
Size: 3945 1156 2949
Fragments arranged by size:
3945 2949 1156
ApoI r'AATT_y
Cuts at: 1493 2734 3716 5359 5585 5737 7846 1493
Size: 1241 982 1643 226 152 2109 1697
Fragments arranged by size:
2109 1697 1643 1241 982 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6604 160
Size: 872 5572 1606
Fragments arranged by size:
5572 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8050
AvaI C'yCGr_G
Cuts at: 1870 1943 2342 3981 5249 5375 1870
Size: 73 399 1639 1268 126 4545
Fragments arranged by size:
4545 1639 1268 399 126 73
BamHI G'GATC_C
Cuts at: 5380 5747 5380
Size: 367 7683
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2086 2272 2351 5371 6826
Size: 325 217 627 299 186 79 3020 1455
Cuts at: 6826 618
Size: 1842
Fragments arranged by size:
3020 1842 1455 627 325 299 217 186
79
BanII G_rGCy'C
Cuts at: 1391 2233 2333 2631 3126 4806 5169 5250 5369
Size: 842 100 298 495 1680 363 81 119
Cuts at: 5369 1391
Size: 4072
Fragments arranged by size:
4072 1680 842 495 363 298 119 100
81
BbeI G_GCGC'C
Cuts at: 2276 2355 2276
Size: 79 7971
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2826 3574 3777 4066 4135 928
Size: 971 927 748 203 289 69 4843
Fragments arranged by size:
4843 971 927 748 289 203 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6241 6275 1903
Size: 34 4304 34 3678
Fragments arranged by size:
4304 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3017 4613 5942 7152 3017
Size: 1596 1329 1210 3915
Fragments arranged by size:
3915 1596 1329 1210
BclI T'GATC_A
Cuts at: 1548 2664 1548
Size: 1116 6934
BfrBI ATG'CAT
Cuts at: 4444 4444
Size: 8050
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2468 3121 3621
Size: 107 122 71 631 605 796 653 500
Cuts at: 3621 3884 6661 136
Size: 263 2777 1525
Fragments arranged by size:
2777 1525 796 653 631 605 500 263
122 107 71
BglII A'GATC_T
Cuts at: 4320 8045 4320
Size: 3725 4325
BlpI GC'TnA_GC
Cuts at: 2033 5467 2033
Size: 3434 4616
Bme1580I G_kGCm'C
Cuts at: 1790 2091 2142 2166 3617 3711 3840 6111 7267
Size: 301 51 24 1451 94 129 2271 1156
Cuts at: 7267 1790
Size: 2573
Fragments arranged by size:
2573 2271 1451 1156 301 129 94 51
24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8050
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3028 3855 5781
Size: 416 53 83 399 24 1611 827 1926
Cuts at: 5781 6739 442
Size: 958 1753
Fragments arranged by size:
1926 1753 1611 958 827 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8050
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5333 6710 1239
Size: 4094 1377 2579
Fragments arranged by size:
4094 2579 1377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2111 2947 2969 3568 6175 7040 7338 2111
Size: 836 22 599 2607 865 298 2823
Fragments arranged by size:
2823 2607 865 836 599 298 22
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6713 882
Size: 617 5214 2219
Fragments arranged by size:
5214 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3169 5841 493
Size: 711 1965 2672 2702
Fragments arranged by size:
2702 2672 1965 711
BsaBI GATnn'nnATC
Cuts at: 2462 2669 8044 2462
Size: 207 5375 2468
Fragments arranged by size:
5375 2468 207
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2273 2352 2382 4524
Size: 53 83 186 936 740 79 30 2142
Cuts at: 4524 5857 6239 6874 275
Size: 1333 382 635 1451
Fragments arranged by size:
2142 1451 1333 936 740 635 382 186
83 79 53 30
BsaWI w'CCGG_w
Cuts at: 2209 6483 6881 7155 7302 7713 2209
Size: 4274 398 274 147 411 2546
Fragments arranged by size:
4274 2546 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2002 2032 2354 2384 2522
Size: 30 165 30 599 30 322 30 138
Cuts at: 2522 2552 2894 2924 3979 4009 1178
Size: 30 342 30 1055 30 5219
Fragments arranged by size:
5219 1055 599 342 322 165 138 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2086 2089 2494 2834 5330 1186
Size: 837 63 3 405 340 2496 3906
Fragments arranged by size:
3906 2496 837 405 340 63 3
BseYI C'CCAG_C
Cuts at: 2090 3070 3245 4039 5116 7253 7749 2090
Size: 980 175 794 1077 2137 496 2391
Fragments arranged by size:
2391 2137 1077 980 794 496 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2636 3897 4043 1163
Size: 573 900 1261 146 5170
Fragments arranged by size:
5170 1261 900 573 146
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5500 6261 7289 1540
Size: 275 186 3499 761 1028 2301
Fragments arranged by size:
3499 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2166 2306 2631 3165 4863 5169 5250 6111 6196
Size: 140 325 534 1698 306 81 861 85
Cuts at: 6196 7267 2166
Size: 1071 2949
Fragments arranged by size:
2949 1698 1071 861 534 325 306 140
85 81
BsmI GAATG_Cn'
Cuts at: 5561 5654 5561
Size: 93 7957
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2142 2166 2233 2306 2333 2631
Size: 399 301 51 24 67 73 27 298
Cuts at: 2631 3126 3165 3617 3711 3840 4806 4863 5169
Size: 495 39 452 94 129 966 57 306
Cuts at: 5169 5250 5369 6111 6196 7267 1391
Size: 81 119 742 85 1071 2174
Fragments arranged by size:
2174 1071 966 742 495 452 399 306
301 298 129 119 94 85 81 73
67 57 51 39 27 24
BspEI T'CCGG_A
Cuts at: 2209 7713 2209
Size: 5504 2546
BspHI T'CATG_A
Cuts at: 5065 5939 7669 5065
Size: 874 1730 5446
Fragments arranged by size:
5446 1730 874
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5405 844
Size: 4561 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5937 1394
Size: 429 4114 3507
Fragments arranged by size:
4114 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3126 6545 6719 65
Size: 1183 1878 3419 174 1396
Fragments arranged by size:
3419 1878 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6694 6881 1700
Size: 110 4884 187 2869
Fragments arranged by size:
4884 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8050
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8050
BssSI C'ACGA_G
Cuts at: 2681 6110 7122 2681
Size: 3429 1012 3609
Fragments arranged by size:
3609 3429 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8050
BstBI TT'CG_AA
Cuts at: 3021 6854 3021
Size: 3833 4217
BstEII G'GTnAC_C
Cuts at: 1184 3556 1184
Size: 2372 5678
BstXI CCAn_nnnn'nTGG
Cuts at: 3961 4026 4221 5002 3961
Size: 65 195 781 7009
Fragments arranged by size:
7009 781 195 65
BstYI r'GATC_y
Cuts at: 1972 2121 2940 4320 5127 5380 5747 6132 6149
Size: 149 819 1380 807 253 367 385 17
Cuts at: 6149 7601 7687 7699 8045 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1380 819 807 385 367 346
253 149 86 17 12
BstZ17I GTA'TAC
Cuts at: 4870 4870
Size: 8050
Bsu36I CC'TnA_GG
Cuts at: 3118 3118
Size: 8050
BtgI C'CryG_G
Cuts at: 513 1065 2375 2397 2519 2565 2690 3420 513
Size: 552 1310 22 122 46 125 730 5143
Fragments arranged by size:
5143 1310 730 552 125 122 46 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7311
Fragments arranged by size:
7311 683 56
BtsI GCAGTG_nn'
Cuts at: 2533 4065 4411 5566 6360 6380 2533
Size: 1532 346 1155 794 20 4203
Fragments arranged by size:
4203 1532 1155 794 346 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8050
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8015
DraI TTT'AAA
Cuts at: 5354 5354
Size: 8050
DraIII CAC_nnn'GTG
Cuts at: 2146 2146
Size: 8050
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2263 5344 7057 809
Size: 507 947 3081 1713 1802
Fragments arranged by size:
3081 1802 1713 947 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2196 2314
Size: 54 1425 50 275 18 249 117 118
Cuts at: 2314 2372 2460 3491 3622 4016 4200 5497 6386
Size: 58 88 1031 131 394 184 1297 889
Cuts at: 6386 8015 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1297 1031 889 394 275 249
184 131 118 117 88 58 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5497 1537
Size: 275 3685 4090
Fragments arranged by size:
4090 3685 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3661 4091 4323 5155 5980 1353
Size: 2308 430 232 832 825 3423
Fragments arranged by size:
3423 2308 832 825 430 232
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2375 3028 6637 7011 7157 387
Size: 1064 138 786 653 3609 374 146 1280
Fragments arranged by size:
3609 1280 1064 786 653 374 146 138
EcoICRI GAG'CTC
Cuts at: 2629 5167 5248 2629
Size: 2538 81 5431
Fragments arranged by size:
5431 2538 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3273 4140 4431 4520 5333 6113 6710
Size: 393 1641 867 291 89 813 780 597
Cuts at: 6710 7497 7765 1239
Size: 787 268 1524
Fragments arranged by size:
1641 1524 867 813 787 780 597 393
291 268 89
EcoNI CCTnn'n_nnAGG
Cuts at: 4371 4371
Size: 8050
EcoO109I rG'GnC_Cy
Cuts at: 2365 2920 3978 5024 2365
Size: 555 1058 1046 5391
Fragments arranged by size:
5391 1058 1046 555
EcoRI G'AATT_C
Cuts at: 5359 5737 5359
Size: 378 7672
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3562 5355 3562
Size: 1793 6257
FspI TGC'GCA
Cuts at: 2068 6556 2068
Size: 4488 3562
HaeII r_GCGC'y
Cuts at: 1678 2276 2355 2591 7197 1678
Size: 598 79 236 4606 2531
Fragments arranged by size:
4606 2531 598 236 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2522 2554 2689 2721 3094
Size: 32 135 32 950 32 135 32 373
Cuts at: 3094 3126 3556 3588 6691 6723 6765 6797 1373
Size: 32 430 32 3103 32 42 32 2626
Fragments arranged by size:
3103 2626 950 430 373 135 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5394 5640 669
Size: 988 281 3456 246 3079
Fragments arranged by size:
3456 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8050
HpaI GTT'AAC
Cuts at: 5640 5640
Size: 8050
KasI G'GCGC_C
Cuts at: 2272 2351 2272
Size: 79 7971
KpnI G_GTAC'C
Cuts at: 5375 5375
Size: 8050
MfeI C'AATT_G
Cuts at: 5649 5649
Size: 8050
MluI A'CGCG_T
Cuts at: 7926 7926
Size: 8050
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3247 3550 4202 5315 7164 7348 1305
Size: 1942 303 652 1113 1849 184 2007
Fragments arranged by size:
2007 1942 1849 1113 652 303 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2374 2462 3493 3624 4018
Size: 54 1425 343 542 88 1031 131 394
Cuts at: 4018 4202 10
Size: 184 3858
Fragments arranged by size:
3858 1425 1031 542 394 343 184 131
88 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2660 2978 3266 3971 6008 6367 6526
Size: 648 1494 318 288 705 2037 359 159
Cuts at: 6526 518
Size: 2042
Fragments arranged by size:
2042 2037 1494 705 648 359 318 288
159
MspA1I CmG'CkG
Cuts at: 1606 3453 5116 5287 5466 6143 7291 7536 1606
Size: 1847 1663 171 179 677 1148 245 2120
Fragments arranged by size:
2120 1847 1663 1148 677 245 179 171
NarI GG'CG_CC
Cuts at: 2273 2352 2273
Size: 79 7971
NcoI C'CATG_G
Cuts at: 513 1065 2519 3420 513
Size: 552 1454 901 5143
Fragments arranged by size:
5143 1454 901 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8050
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8050
NotI GC'GGCC_GC
Cuts at: 5497 5497
Size: 8050
NsiI A_TGCA'T
Cuts at: 4446 4446
Size: 8050
NspI r_CATG'y
Cuts at: 1132 1229 5408 6953 1132
Size: 97 4179 1545 2229
Fragments arranged by size:
4179 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8050
PciI A'CATG_T
Cuts at: 6949 6949
Size: 8050
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2566 3088 1108
Size: 1458 522 6070
Fragments arranged by size:
6070 1458 522
PmeI GTTT'AAAC
Cuts at: 5354 5354
Size: 8050
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3222 3254 6119 6151 7632 7664 3222
Size: 32 2865 32 1481 32 3608
Fragments arranged by size:
3608 2865 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2920 3978 5024 2920
Size: 1058 1046 5946
Fragments arranged by size:
5946 1058 1046
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8050
PsiI TTA'TAA
Cuts at: 5620 5620
Size: 8050
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2774 2806 2774
Size: 32 8018
PstI C_TGCA'G
Cuts at: 830 5286 5402 830
Size: 4456 116 3478
Fragments arranged by size:
4456 3478 116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8050
PvuII CAG'CTG
Cuts at: 3453 5116 5287 3453
Size: 1663 171 6216
Fragments arranged by size:
6216 1663 171
SacI G_AGCT'C
Cuts at: 2631 5169 5250 2631
Size: 2538 81 5431
Fragments arranged by size:
5431 2538 81
SalI G'TCGA_C
Cuts at: 1936 5392 1936
Size: 3456 4594
SanDI GG'GwC_CC
Cuts at: 5024 5024
Size: 8050
SapI GCTCTTCn'nnn_
Cuts at: 4323 4323
Size: 8050
SbfI CC_TGCA'GG
Cuts at: 5402 5402
Size: 8050
ScaI AGT'ACT
Cuts at: 6298 8010 6298
Size: 1712 6338
SfcI C'TryA_G
Cuts at: 826 1045 2936 3201 5282 5398 6533 7214 7405
Size: 219 1891 265 2081 116 1135 681 191
Cuts at: 7405 826
Size: 1471
Fragments arranged by size:
2081 1891 1471 1135 681 265 219 191
116
SfoI GGC'GCC
Cuts at: 2274 2353 2274
Size: 79 7971
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8050
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8050
SmaI CCC'GGG
Cuts at: 1872 2344 5377 1872
Size: 472 3033 4545
Fragments arranged by size:
4545 3033 472
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2126 2926 2948 3547 5249 6154
Size: 197 926 183 800 22 599 1702 905
Cuts at: 6154 7055 7317 820
Size: 901 262 1553
Fragments arranged by size:
1702 1553 926 905 901 800 599 262
197 183 22
SnaBI TAC'GTA
Cuts at: 493 3169 5841 493
Size: 2676 2672 2702
Fragments arranged by size:
2702 2676 2672
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8050
SphI G_CATG'C
Cuts at: 1132 5408 1132
Size: 4276 3774
SspI AAT'ATT
Cuts at: 5 52 3299 5974 5
Size: 47 3247 2675 2081
Fragments arranged by size:
3247 2675 2081 47
StuI AGG'CCT
Cuts at: 1517 2475 3336 1517
Size: 958 861 6231
Fragments arranged by size:
6231 958 861
StyI C'CwwG_G
Cuts at: 513 1065 2519 2875 3420 4119 4372 5153 5489
Size: 552 1454 356 545 699 253 781 336
Cuts at: 5489 513
Size: 3074
Fragments arranged by size:
3074 1454 781 699 552 545 356 336
253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2930 2986 3055 3845 4654 6077 6094
Size: 1255 1350 56 69 790 809 1423 17
Cuts at: 6094 6247 6432 6877 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1423 1350 1255 809 790 445 185
153 69 56 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6296 8008 96
Size: 274 80 33 51 1443 4319 1712 138
Fragments arranged by size:
4319 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2354 2386 8010 8042 2354
Size: 32 5624 32 2362
Fragments arranged by size:
5624 2362 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2636 5028 1316
Size: 1320 2392 4338
Fragments arranged by size:
4338 2392 1320
XbaI T'CTAG_A
Cuts at: 3634 5386 3634
Size: 1752 6298
XhoI C'TCGA_G
Cuts at: 1943 5249 1943
Size: 3306 4744
XmaI C'CCGG_G
Cuts at: 1870 2342 5375 1870
Size: 472 3033 4545
Fragments arranged by size:
4545 3033 472
XmnI GAAnn'nnTTC
Cuts at: 6179 6179
Size: 8050
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5858 276
Size: 53 83 186 936 4324 2468
Fragments arranged by size:
4324 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsiWI BsmBI EcoRV FseI FspAI NaeI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII
SacII SexAI SfiI SrfI SwaI XcmI