Restriction map for
pFN21AA1966
(Circular) (Six-base) MAPSORT of: pFN21AA1966.seq Check: 2173 from: 1 to: 6886
pFN21AA1966 6886 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 4696 278
Size: 53 83 186 936 2035 1125 2468
Fragments arranged by size:
2468 2035 1125 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3610 4033 4229 1937
Size: 1673 423 196 4594
Fragments arranged by size:
4594 1673 423 196
Acc65I G'GTAC_C
Cuts at: 4207 4207
Size: 6886
AclI AA'CG_TT
Cuts at: 1563 5013 5386 1563
Size: 3450 373 3063
Fragments arranged by size:
3450 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2088 2553 3237 4949 6333 6601 1632
Size: 456 465 684 1712 1384 268 1917
Fragments arranged by size:
1917 1712 1384 684 465 456 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6689
AflIII A'CryG_T
Cuts at: 1203 5785 6762 1203
Size: 4582 977 1327
Fragments arranged by size:
4582 1327 977
AgeI A'CCGG_T
Cuts at: 5717 5717
Size: 6886
AhdI GACnn_n'nnGTC
Cuts at: 5615 5615
Size: 6886
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6886
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6886
AlwNI CAG_nnn'CTG
Cuts at: 4629 6201 4629
Size: 1572 5314
ApaLI G'TGCA_C
Cuts at: 4943 6099 4943
Size: 1156 5730
ApoI r'AATT_y
Cuts at: 1493 2346 3227 3788 4195 4421 4573 6682 1493
Size: 853 881 561 407 226 152 2109 1697
Fragments arranged by size:
2109 1697 881 853 561 407 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5440 160
Size: 872 4408 1606
Fragments arranged by size:
4408 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
AvaI C'yCGr_G
Cuts at: 1870 1943 4211 1870
Size: 73 2268 4545
Fragments arranged by size:
4545 2268 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2055 2088 2055
Size: 33 6853
BamHI G'GATC_C
Cuts at: 2419 3700 4216 4583 2419
Size: 1281 516 367 4722
Fragments arranged by size:
4722 1281 516 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4207 5662 618
Size: 325 217 627 2420 1455 1842
Fragments arranged by size:
2420 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2231 4205 1391
Size: 840 1974 4072
Fragments arranged by size:
4072 1974 840
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2502 3464 928
Size: 971 603 962 4350
Fragments arranged by size:
4350 971 962 603
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5077 5111 1903
Size: 34 3140 34 3678
Fragments arranged by size:
3678 3140 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4778 5988 4778
Size: 1210 5676
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6886
BfrBI ATG'CAT
Cuts at: 3544 3544
Size: 6886
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5497 136
Size: 107 122 71 631 605 337 3488 1525
Fragments arranged by size:
3488 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2309 2861 3179 6881 2309
Size: 552 318 3702 2314
Fragments arranged by size:
3702 2314 552 318
BlpI GC'TnA_GC
Cuts at: 4303 4303
Size: 6886
Bme1580I G_kGCm'C
Cuts at: 1790 4947 6103 1790
Size: 3157 1156 2573
Fragments arranged by size:
3157 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2489 3428 4112 1318
Size: 1171 939 684 4092
Fragments arranged by size:
4092 1171 939 684
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3295 4617 5575
Size: 416 53 83 399 24 1878 1322 958
Cuts at: 5575 442
Size: 1753
Fragments arranged by size:
1878 1753 1322 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6886
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2353 5546 1239
Size: 1114 3193 2579
Fragments arranged by size:
3193 2579 1114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3879 5011 5876 6174 3879
Size: 1132 865 298 4591
Fragments arranged by size:
4591 1132 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2763 3676 4041 4059 4149 5549 882
Size: 617 1264 913 365 18 90 1400 2219
Fragments arranged by size:
2219 1400 1264 913 617 365 90 18
BsaAI yAC'GTr
Cuts at: 493 1204 4677 493
Size: 711 3473 2702
Fragments arranged by size:
3473 2702 711
BsaBI GATnn'nnATC
Cuts at: 2337 6880 2337
Size: 4543 2343
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3568 3607 4693 5075
Size: 53 83 186 936 2035 39 1086 382
Cuts at: 5075 5710 275
Size: 635 1451
Fragments arranged by size:
2035 1451 1086 936 635 382 186 83
53 39
BsaWI w'CCGG_w
Cuts at: 2409 5319 5717 5991 6138 6549 2409
Size: 2910 398 274 147 411 2746
Fragments arranged by size:
2910 2746 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6661
Fragments arranged by size:
6661 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2621 2708 2711 2714 2723 2726 2729
Size: 853 582 87 3 3 9 3 3
Cuts at: 2729 2738 2741 2744 2747 2750 2795 3882 1186
Size: 9 3 3 3 3 45 1087 4190
Fragments arranged by size:
4190 1087 853 582 87 45 9 9
3 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 3319 3462 6089 6585 3319
Size: 143 2627 496 3620
Fragments arranged by size:
3620 2627 496 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6313
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4336 5097 6125 1540
Size: 275 186 2335 761 1028 2301
Fragments arranged by size:
2335 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2231 4947 5032 6103 2231
Size: 2716 85 1071 3014
Fragments arranged by size:
3014 2716 1071 85
BsmI GAATG_Cn'
Cuts at: 3544 3546 3831 4397 4490 3544
Size: 2 285 566 93 5940
Fragments arranged by size:
5940 566 285 93 2
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2231 4205 4947 5032 6103 1391
Size: 399 441 1974 742 85 1071 2174
Fragments arranged by size:
2174 1974 1071 742 441 399 85
BspEI T'CCGG_A
Cuts at: 2409 6549 2409
Size: 4140 2746
BspHI T'CATG_A
Cuts at: 4775 6505 4775
Size: 1730 5156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2966 4241 844
Size: 2122 1275 3489
Fragments arranged by size:
3489 2122 1275
BsrBI CCG'CTC
Cuts at: 1394 1823 4773 1394
Size: 429 2950 3507
Fragments arranged by size:
3507 2950 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5381 5555 65
Size: 1183 4133 174 1396
Fragments arranged by size:
4133 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5530 5717 1700
Size: 110 3720 187 2869
Fragments arranged by size:
3720 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6886
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6886
BssSI C'ACGA_G
Cuts at: 3561 4946 5958 3561
Size: 1385 1012 4489
Fragments arranged by size:
4489 1385 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3826 794
Size: 3032 3854
BstBI TT'CG_AA
Cuts at: 5690 5690
Size: 6886
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6886
BstXI CCAn_nnnn'nTGG
Cuts at: 2558 3816 2558
Size: 1258 5628
BstYI r'GATC_y
Cuts at: 1972 2309 2419 2498 2861 3179 3272 3700 4216
Size: 337 110 79 363 318 93 428 516
Cuts at: 4216 4583 4968 4985 6437 6523 6535 6881 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 516 428 385 367 363 346
337 318 110 93 86 79 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5395
Fragments arranged by size:
5395 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6147
Fragments arranged by size:
6147 683 56
BtsI GCAGTG_nn'
Cuts at: 2572 4402 5196 5216 2572
Size: 1830 794 20 4242
Fragments arranged by size:
4242 1830 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6886
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6851
DraI TTT'AAA
Cuts at: 3334 3696 3759 4190 3334
Size: 362 63 431 6030
Fragments arranged by size:
6030 431 362 63
DraIII CAC_nnn'GTG
Cuts at: 3289 3289
Size: 6886
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5893 809
Size: 507 4577 1802
Fragments arranged by size:
4577 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4021 4333 5222
Size: 54 1425 50 275 18 2191 312 889
Cuts at: 5222 6851 8
Size: 1629 43
Fragments arranged by size:
2191 1629 1425 889 312 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4333 1537
Size: 275 2521 4090
Fragments arranged by size:
4090 2521 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2667 2700 2715 2903 4177 4816 1353
Size: 1314 33 15 188 1274 639 3423
Fragments arranged by size:
3423 1314 1274 639 188 33 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5473 5847 5993 387
Size: 1064 138 3884 374 146 1280
Fragments arranged by size:
3884 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2229 2229
Size: 6886
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 2353 2553 3237 4949 5546 6333
Size: 393 456 265 200 684 1712 597 787
Cuts at: 6333 6601 1239
Size: 268 1524
Fragments arranged by size:
1712 1524 787 684 597 456 393 268
265 200
EcoNI CCTnn'n_nnAGG
Cuts at: 3819 3837 3912 3819
Size: 18 75 6793
Fragments arranged by size:
6793 75 18
EcoO109I rG'GnC_Cy
Cuts at: 3765 3765
Size: 6886
EcoRI G'AATT_C
Cuts at: 4195 4573 4195
Size: 378 6508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3267 3267
Size: 6886
FspI TGC'GCA
Cuts at: 5392 5392
Size: 6886
HaeII r_GCGC'y
Cuts at: 1678 6033 1678
Size: 4355 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2987 3019 3063 3092 3095
Size: 32 135 32 1415 32 44 29 3
Cuts at: 3095 3124 3573 3605 3646 3678 5527 5559 5601
Size: 29 449 32 41 32 1849 32 42
Cuts at: 5601 5633 1373
Size: 32 2626
Fragments arranged by size:
2626 1849 1415 449 135 44 42 41
32 32 32 32 32 32 32 29
29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3611 4230 4476 669
Size: 988 281 1673 619 246 3079
Fragments arranged by size:
3079 1673 988 619 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6886
HpaI GTT'AAC
Cuts at: 4476 4476
Size: 6886
KpnI G_GTAC'C
Cuts at: 4211 4211
Size: 6886
MfeI C'AATT_G
Cuts at: 2931 4485 2931
Size: 1554 5332
MluI A'CGCG_T
Cuts at: 6762 6762
Size: 6886
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6000 6184 1305
Size: 4695 184 2007
Fragments arranged by size:
4695 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5064
Fragments arranged by size:
5064 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3102 3969 4844 5203 5362 518
Size: 648 1936 867 875 359 159 2042
Fragments arranged by size:
2042 1936 875 867 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4302 4979 6127 6372 1606
Size: 2696 677 1148 245 2120
Fragments arranged by size:
2696 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5395
Fragments arranged by size:
5395 939 552
NdeI CA'TA_TG
Cuts at: 387 2971 387
Size: 2584 4302
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6886
NotI GC'GGCC_GC
Cuts at: 4333 4333
Size: 6886
NsiI A_TGCA'T
Cuts at: 3546 3546
Size: 6886
NspI r_CATG'y
Cuts at: 1132 1229 4244 5789 1132
Size: 97 3015 1545 2229
Fragments arranged by size:
3015 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6886
PciI A'CATG_T
Cuts at: 5785 5785
Size: 6886
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3396 3718 1108
Size: 2288 322 4276
Fragments arranged by size:
4276 2288 322
PfoI T'CCnGG_A
Cuts at: 3591 3591
Size: 6886
PmeI GTTT'AAAC
Cuts at: 4190 4190
Size: 6886
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4955 4987 6468 6500 4955
Size: 32 1481 32 5341
Fragments arranged by size:
5341 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3765 3765
Size: 6886
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6886
PsiI TTA'TAA
Cuts at: 2382 4456 2382
Size: 2074 4812
PstI C_TGCA'G
Cuts at: 830 4238 830
Size: 3408 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6886
SacI G_AGCT'C
Cuts at: 2231 2231
Size: 6886
SalI G'TCGA_C
Cuts at: 1936 3609 4228 1936
Size: 1673 619 4594
Fragments arranged by size:
4594 1673 619
SbfI CC_TGCA'GG
Cuts at: 4238 4238
Size: 6886
ScaI AGT'ACT
Cuts at: 5134 6846 5134
Size: 1712 5174
SfcI C'TryA_G
Cuts at: 826 1045 4234 5369 6050 6241 826
Size: 219 3189 1135 681 191 1471
Fragments arranged by size:
3189 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6886
SmaI CCC'GGG
Cuts at: 1872 4213 1872
Size: 2341 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3894 4990 5891 6153 820
Size: 197 926 1951 1096 901 262 1553
Fragments arranged by size:
1951 1553 1096 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4677 493
Size: 4184 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6886
SphI G_CATG'C
Cuts at: 1132 4244 1132
Size: 3112 3774
SspI AAT'ATT
Cuts at: 5 52 3204 4810 5
Size: 47 3152 1606 2081
Fragments arranged by size:
3152 2081 1606 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6886
StyI C'CwwG_G
Cuts at: 513 1065 2004 3395 3650 3815 4325 513
Size: 552 939 1391 255 165 510 3074
Fragments arranged by size:
3074 1391 939 552 510 255 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3277 4173 4913 4930 5083 5268 5713
Size: 1255 1697 896 740 17 153 185 445
Cuts at: 5713 325
Size: 1498
Fragments arranged by size:
1697 1498 1255 896 740 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2261 3961 5132
Size: 274 80 33 51 1443 284 1700 1171
Cuts at: 5132 6844 96
Size: 1712 138
Fragments arranged by size:
1712 1700 1443 1171 284 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6846 6878 6846
Size: 32 6854
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6886
XbaI T'CTAG_A
Cuts at: 2329 4222 2329
Size: 1893 4993
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3813 3813
Size: 6886
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6886
XmaI C'CCGG_G
Cuts at: 1870 4211 1870
Size: 2341 4545
XmnI GAAnn'nnTTC
Cuts at: 3544 5015 3544
Size: 1471 5415
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 4694 276
Size: 53 83 186 936 2035 1125 2468
Fragments arranged by size:
2468 2035 1125 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI