Restriction map for
pFN21AA1962
(Circular) (Six-base) MAPSORT of: pFN21AA1962.seq Check: 2434 from: 1 to: 6937
pFN21AA1962 6937 bp
With 182 enzymes: SgfI *
March 28, 2012 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4747 278
Size: 53 83 186 936 3211 2468
Fragments arranged by size:
3211 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2973 4280 1937
Size: 1036 1307 4594
Fragments arranged by size:
4594 1307 1036
Acc65I G'GTAC_C
Cuts at: 4258 4258
Size: 6937
AclI AA'CG_TT
Cuts at: 1563 5064 5437 1563
Size: 3501 373 3063
Fragments arranged by size:
3501 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2931 3004 3102 3667 4003 5000 6384 6652
Size: 1299 73 98 565 336 997 1384 268
Cuts at: 6652 1632
Size: 1917
Fragments arranged by size:
1917 1384 1299 997 565 336 268 98
73
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6740
AflIII A'CryG_T
Cuts at: 1203 3659 5836 6813 1203
Size: 2456 2177 977 1327
Fragments arranged by size:
2456 2177 1327 977
AgeI A'CCGG_T
Cuts at: 5768 5768
Size: 6937
AhdI GACnn_n'nnGTC
Cuts at: 5666 5666
Size: 6937
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6937
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6937
AlwNI CAG_nnn'CTG
Cuts at: 2245 2305 4680 6252 2245
Size: 60 2375 1572 2930
Fragments arranged by size:
2930 2375 1572 60
ApaLI G'TGCA_C
Cuts at: 4994 6150 4994
Size: 1156 5781
ApoI r'AATT_y
Cuts at: 1493 2624 2842 2900 3381 3549 3633 3717 3775
Size: 1131 218 58 481 168 84 84 58
Cuts at: 3775 3801 3969 4053 4137 4246 4472 4624 6733
Size: 26 168 84 84 109 226 152 2109
Cuts at: 6733 1493
Size: 1697
Fragments arranged by size:
2109 1697 1131 481 226 218 168 168
152 109 84 84 84 84 58 58
26
AseI AT'TA_AT
Cuts at: 160 1032 5491 160
Size: 872 4459 1606
Fragments arranged by size:
4459 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6937
AvaI C'yCGr_G
Cuts at: 1870 1943 4262 1870
Size: 73 2319 4545
Fragments arranged by size:
4545 2319 73
BamHI G'GATC_C
Cuts at: 4267 4634 4267
Size: 367 6570
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2757 4258 5713 618
Size: 325 217 627 970 1501 1455 1842
Fragments arranged by size:
1842 1501 1455 970 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2232 4256 1391
Size: 841 2024 4072
Fragments arranged by size:
4072 2024 841
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4187 928
Size: 971 2288 3678
Fragments arranged by size:
3678 2288 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5128 5162 1903
Size: 34 50 34 3107 34 3678
Fragments arranged by size:
3678 3107 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4829 6039 4829
Size: 1210 5727
BclI T'GATC_A
Cuts at: 1548 2773 1548
Size: 1225 5712
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5548 136
Size: 107 122 71 631 605 3876 1525
Fragments arranged by size:
3876 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6932 6932
Size: 6937
BlpI GC'TnA_GC
Cuts at: 4354 4354
Size: 6937
Bme1580I G_kGCm'C
Cuts at: 1790 2022 4998 6154 1790
Size: 232 2976 1156 2573
Fragments arranged by size:
2976 2573 1156 232
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6937
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4668 5626 442
Size: 416 53 83 399 24 3251 958 1753
Fragments arranged by size:
3251 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6937
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2473 4050 2473
Size: 1577 5360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2585 3411 3579 3663 3747 3831 5597 1239
Size: 1346 826 168 84 84 84 1766 2579
Fragments arranged by size:
2579 1766 1346 826 168 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2412 2998 5062 5927 6225 2412
Size: 586 2064 865 298 3124
Fragments arranged by size:
3124 2064 865 586 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2242 3586 5600 882
Size: 617 743 1344 2014 2219
Fragments arranged by size:
2219 2014 1344 743 617
BsaAI yAC'GTr
Cuts at: 493 1204 4728 493
Size: 711 3524 2702
Fragments arranged by size:
3524 2702 711
BsaBI GATnn'nnATC
Cuts at: 2772 6931 2772
Size: 4159 2778
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4744 5126 5761 275
Size: 53 83 186 936 3211 382 635 1451
Fragments arranged by size:
3211 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4145 5370 5768 6042 6189 6600 4145
Size: 1225 398 274 147 411 4482
Fragments arranged by size:
4482 1225 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2872 2902 1178
Size: 30 165 30 1469 30 5213
Fragments arranged by size:
5213 1469 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2465 2562 2834 1186
Size: 1279 97 272 5289
Fragments arranged by size:
5289 1279 272 97
BseYI C'CCAG_C
Cuts at: 2664 2796 6140 6636 2664
Size: 132 3344 496 2965
Fragments arranged by size:
3344 2965 496 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6364
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4387 5148 6176 1540
Size: 275 186 2386 761 1028 2301
Fragments arranged by size:
2386 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2232 3529 4047 4998 5083 6154 2232
Size: 1297 518 951 85 1071 3015
Fragments arranged by size:
3015 1297 1071 951 518 85
BsmI GAATG_Cn'
Cuts at: 4448 4541 4448
Size: 93 6844
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2232 3529 4047 4256 4998 5083
Size: 399 232 210 1297 518 209 742 85
Cuts at: 5083 6154 1391
Size: 1071 2174
Fragments arranged by size:
2174 1297 1071 742 518 399 232 210
209 85
BspEI T'CCGG_A
Cuts at: 6600 6600
Size: 6937
BspHI T'CATG_A
Cuts at: 4826 6556 4826
Size: 1730 5207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4292 844
Size: 3448 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4824 1394
Size: 429 3001 3507
Fragments arranged by size:
3507 3001 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5432 5606 65
Size: 1183 4184 174 1396
Fragments arranged by size:
4184 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5581 5768 1700
Size: 110 3771 187 2869
Fragments arranged by size:
3771 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6937
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6937
BssSI C'ACGA_G
Cuts at: 4997 6009 4997
Size: 1012 5925
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6937
BstBI TT'CG_AA
Cuts at: 5741 5741
Size: 6937
BstEII G'GTnAC_C
Cuts at: 1184 2374 1184
Size: 1190 5747
BstYI r'GATC_y
Cuts at: 1972 2416 4267 4634 5019 5036 6488 6574 6586
Size: 444 1851 367 385 17 1452 86 12
Cuts at: 6586 6932 1972
Size: 346 1977
Fragments arranged by size:
1977 1851 1452 444 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2612 2762 2612
Size: 150 6787
BtgI C'CryG_G
Cuts at: 513 1065 2687 513
Size: 552 1622 4763
Fragments arranged by size:
4763 1622 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3429 487
Size: 683 56 2203 3995
Fragments arranged by size:
3995 2203 683 56
BtsI GCAGTG_nn'
Cuts at: 3947 4453 5247 5267 3947
Size: 506 794 20 5617
Fragments arranged by size:
5617 794 506 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6937
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6902
DraI TTT'AAA
Cuts at: 4189 4241 4189
Size: 52 6885
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5944 809
Size: 507 4628 1802
Fragments arranged by size:
4628 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4384 5273 6902
Size: 54 1425 50 275 18 2554 889 1629
Cuts at: 6902 8
Size: 43
Fragments arranged by size:
2554 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4384 1537
Size: 275 2572 4090
Fragments arranged by size:
4090 2572 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2848 3121 4867 1353
Size: 1495 273 1746 3423
Fragments arranged by size:
3423 1746 1495 273
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5524 5898 6044 387
Size: 1064 138 3935 374 146 1280
Fragments arranged by size:
3935 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2230 2230
Size: 6937
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2585 2931 3004 3102 3411 3579 3663
Size: 393 953 346 73 98 309 168 84
Cuts at: 3663 3667 3747 3831 4003 5000 5597 6384 6652
Size: 4 80 84 172 997 597 787 268
Cuts at: 6652 1239
Size: 1524
Fragments arranged by size:
1524 997 953 787 597 393 346 309
268 172 168 98 84 84 80 73
4
EcoO109I rG'GnC_Cy
Cuts at: 2086 3168 2086
Size: 1082 5855
EcoRI G'AATT_C
Cuts at: 3381 3549 3633 3717 3775 3801 3969 4053 4246
Size: 168 84 84 58 26 168 84 193
Cuts at: 4246 4624 3381
Size: 378 5694
Fragments arranged by size:
5694 378 193 168 168 84 84 84
58 26
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3034 3034
Size: 6937
FspI TGC'GCA
Cuts at: 5443 5443
Size: 6937
HaeII r_GCGC'y
Cuts at: 1678 3360 6084 1678
Size: 1682 2724 2531
Fragments arranged by size:
2724 2531 1682
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 2408 2440 3428
Size: 32 135 32 563 32 241 32 988
Cuts at: 3428 3460 3596 3628 3862 3894 5578 5610 5652
Size: 32 136 32 234 32 1684 32 42
Cuts at: 5652 5684 1373
Size: 32 2626
Fragments arranged by size:
2626 1684 988 563 241 234 136 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2219 4281 4527 669
Size: 988 281 281 2062 246 3079
Fragments arranged by size:
3079 2062 988 281 281 246
HindIII A'AGCT_T
Cuts at: 748 3426 748
Size: 2678 4259
HpaI GTT'AAC
Cuts at: 4527 4527
Size: 6937
KpnI G_GTAC'C
Cuts at: 4262 4262
Size: 6937
MfeI C'AATT_G
Cuts at: 3667 4536 3667
Size: 869 6068
MluI A'CGCG_T
Cuts at: 6813 6813
Size: 6937
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3233 6051 6235 1305
Size: 1928 2818 184 2007
Fragments arranged by size:
2818 2007 1928 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5115
Fragments arranged by size:
5115 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3826 3978 4895 5254 5413 518
Size: 648 2660 152 917 359 159 2042
Fragments arranged by size:
2660 2042 917 648 359 159 152
MspA1I CmG'CkG
Cuts at: 1606 4353 5030 6178 6423 1606
Size: 2747 677 1148 245 2120
Fragments arranged by size:
2747 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2687 513
Size: 552 1622 4763
Fragments arranged by size:
4763 1622 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6937
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6937
NotI GC'GGCC_GC
Cuts at: 4384 4384
Size: 6937
NspI r_CATG'y
Cuts at: 1132 1229 3663 4295 5840 1132
Size: 97 2434 632 1545 2229
Fragments arranged by size:
2434 2229 1545 632 97
PacI TTA_AT'TAA
Cuts at: 2958 2958
Size: 6937
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6937
PciI A'CATG_T
Cuts at: 3659 5836 3659
Size: 2177 4760
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6937
PmeI GTTT'AAAC
Cuts at: 4241 4241
Size: 6937
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5006 5038 6519 6551 5006
Size: 32 1481 32 5392
Fragments arranged by size:
5392 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2086 3168 2086
Size: 1082 5855
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6937
PsiI TTA'TAA
Cuts at: 3573 3993 4507 3573
Size: 420 514 6003
Fragments arranged by size:
6003 514 420
PstI C_TGCA'G
Cuts at: 830 4234 4289 830
Size: 3404 55 3478
Fragments arranged by size:
3478 3404 55
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6937
SacI G_AGCT'C
Cuts at: 2232 2232
Size: 6937
SalI G'TCGA_C
Cuts at: 1936 4279 1936
Size: 2343 4594
SbfI CC_TGCA'GG
Cuts at: 4289 4289
Size: 6937
ScaI AGT'ACT
Cuts at: 5185 6897 5185
Size: 1712 5225
SfcI C'TryA_G
Cuts at: 826 1045 2013 3352 4230 4285 5420 6101 6292
Size: 219 968 1339 878 55 1135 681 191
Cuts at: 6292 826
Size: 1471
Fragments arranged by size:
1471 1339 1135 968 878 681 219 191
55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6937
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6937
SmaI CCC'GGG
Cuts at: 1872 4264 1872
Size: 2392 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2427 2977 5041 5942 6204 820
Size: 197 926 484 550 2064 901 262 1553
Fragments arranged by size:
2064 1553 926 901 550 484 262 197
SnaBI TAC'GTA
Cuts at: 493 4728 493
Size: 4235 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6937
SphI G_CATG'C
Cuts at: 1132 4295 1132
Size: 3163 3774
SspI AAT'ATT
Cuts at: 5 52 2804 4861 5
Size: 47 2752 2057 2081
Fragments arranged by size:
2752 2081 2057 47
StuI AGG'CCT
Cuts at: 1517 4203 1517
Size: 2686 4251
StyI C'CwwG_G
Cuts at: 513 1065 2687 4376 513
Size: 552 1622 1689 3074
Fragments arranged by size:
3074 1689 1622 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4964 4981 5134 5319 5764 325
Size: 1255 3384 17 153 185 445 1498
Fragments arranged by size:
3384 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5183 6895 96
Size: 274 80 33 51 1443 3206 1712 138
Fragments arranged by size:
3206 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2470 2502 6897 6929 2470
Size: 32 4395 32 2478
Fragments arranged by size:
4395 2478 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6937
XbaI T'CTAG_A
Cuts at: 4273 4273
Size: 6937
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2804 2804
Size: 6937
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6937
XmaI C'CCGG_G
Cuts at: 1870 4262 1870
Size: 2392 4545
XmnI GAAnn'nnTTC
Cuts at: 3017 3485 3512 3932 4016 5066 3017
Size: 468 27 420 84 1050 4888
Fragments arranged by size:
4888 1050 468 420 84 27
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4745 276
Size: 53 83 186 936 3211 2468
Fragments arranged by size:
3211 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PacI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI