Restriction map for
pFN21AA1948
(Circular) (Six-base) MAPSORT of: pFN21AA1948.seq Check: 814 from: 1 to: 6301
pFN21AA1948 6301 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4111 278
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3644 1937
Size: 1707 4594
Acc65I G'GTAC_C
Cuts at: 3622 3622
Size: 6301
AclI AA'CG_TT
Cuts at: 1563 4428 4801 1563
Size: 2865 373 3063
Fragments arranged by size:
3063 2865 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2401 2608 3280 4364 5748 6016 1632
Size: 769 207 672 1084 1384 268 1917
Fragments arranged by size:
1917 1384 1084 769 672 268 207
AflII C'TTAA_G
Cuts at: 820 1017 2339 820
Size: 197 1322 4782
Fragments arranged by size:
4782 1322 197
AflIII A'CryG_T
Cuts at: 1203 5200 6177 1203
Size: 3997 977 1327
Fragments arranged by size:
3997 1327 977
AgeI A'CCGG_T
Cuts at: 5132 5132
Size: 6301
AhdI GACnn_n'nnGTC
Cuts at: 5030 5030
Size: 6301
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6301
AlwNI CAG_nnn'CTG
Cuts at: 4044 5616 4044
Size: 1572 4729
ApaLI G'TGCA_C
Cuts at: 4358 5514 4358
Size: 1156 5145
ApoI r'AATT_y
Cuts at: 1493 2293 2574 3078 3162 3498 3582 3610 3836
Size: 800 281 504 84 336 84 28 226
Cuts at: 3836 3988 6097 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 800 504 336 281 226 152
84 84 28
AseI AT'TA_AT
Cuts at: 160 1032 4855 160
Size: 872 3823 1606
Fragments arranged by size:
3823 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
AvaI C'yCGr_G
Cuts at: 1870 1943 3626 1870
Size: 73 1683 4545
Fragments arranged by size:
4545 1683 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2404 2437 2404
Size: 33 6268
BamHI G'GATC_C
Cuts at: 3631 3998 3631
Size: 367 5934
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3622 5077 618
Size: 325 217 627 1835 1455 1842
Fragments arranged by size:
1842 1835 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3620 1391
Size: 2229 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2227 928
Size: 971 328 5002
Fragments arranged by size:
5002 971 328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4492 4526 1903
Size: 34 2555 34 3678
Fragments arranged by size:
3678 2555 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2047 2405 4193 5403 2047
Size: 358 1788 1210 2945
Fragments arranged by size:
2945 1788 1210 358
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6301
BfrBI ATG'CAT
Cuts at: 2746 2746
Size: 6301
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4912 136
Size: 107 122 71 631 605 3240 1525
Fragments arranged by size:
3240 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2722 6296 2722
Size: 3574 2727
BlpI GC'TnA_GC
Cuts at: 3718 3718
Size: 6301
Bme1580I G_kGCm'C
Cuts at: 1790 4362 5518 1790
Size: 2572 1156 2573
Fragments arranged by size:
2573 2572 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6301
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2147 2296 4032
Size: 416 53 83 399 24 730 149 1736
Cuts at: 4032 4990 442
Size: 958 1753
Fragments arranged by size:
1753 1736 958 730 416 399 149 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2037 4961 1239
Size: 798 2924 2579
Fragments arranged by size:
2924 2579 798
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2388 4426 5291 5589 2388
Size: 2038 865 298 3100
Fragments arranged by size:
3100 2038 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2137 2234 2246 2351 2392 4964 882
Size: 617 638 97 12 105 41 2572 2219
Fragments arranged by size:
2572 2219 638 617 105 97 41 12
BsaAI yAC'GTr
Cuts at: 493 1204 4092 493
Size: 711 2888 2702
Fragments arranged by size:
2888 2702 711
BsaBI GATnn'nnATC
Cuts at: 6295 6295
Size: 6301
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4108 4490 5125 275
Size: 53 83 186 936 2575 382 635 1451
Fragments arranged by size:
2575 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4734 5132 5406 5553 5964 4734
Size: 398 274 147 411 5071
Fragments arranged by size:
5071 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2231 2261 3051 3081 1178
Size: 30 165 30 828 30 790 30 4398
Fragments arranged by size:
4398 828 790 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6301
BseYI C'CCAG_C
Cuts at: 5504 6000 5504
Size: 496 5805
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2619 2739 1163
Size: 573 883 120 4725
Fragments arranged by size:
4725 883 573 120
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3751 4512 5540 1540
Size: 275 186 1750 761 1028 2301
Fragments arranged by size:
2301 1750 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4362 4447 5518 4362
Size: 85 1071 5145
Fragments arranged by size:
5145 1071 85
BsmI GAATG_Cn'
Cuts at: 2942 3035 3203 3287 3414 3812 3905 2942
Size: 93 168 84 127 398 93 5338
Fragments arranged by size:
5338 398 168 127 93 93 84
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3620 4362 4447 5518 1391
Size: 399 1830 742 85 1071 2174
Fragments arranged by size:
2174 1830 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5964 5964
Size: 6301
BspHI T'CATG_A
Cuts at: 4190 5920 4190
Size: 1730 4571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3656 844
Size: 2812 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4188 1394
Size: 429 2365 3507
Fragments arranged by size:
3507 2365 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4796 4970 65
Size: 1183 3548 174 1396
Fragments arranged by size:
3548 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4945 5132 1700
Size: 110 3135 187 2869
Fragments arranged by size:
3135 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2094 96
Size: 1998 4303
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6301
BssSI C'ACGA_G
Cuts at: 4361 5373 4361
Size: 1012 5289
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6301
BstBI TT'CG_AA
Cuts at: 5105 5105
Size: 6301
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6301
BstXI CCAn_nnnn'nTGG
Cuts at: 2061 2061
Size: 6301
BstYI r'GATC_y
Cuts at: 1972 2722 3631 3998 4383 4400 5852 5938 5950
Size: 750 909 367 385 17 1452 86 12
Cuts at: 5950 6296 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 909 750 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2332 3133 513
Size: 552 939 328 801 3681
Fragments arranged by size:
3681 939 801 552 328
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5562
Fragments arranged by size:
5562 683 56
BtsI GCAGTG_nn'
Cuts at: 3817 4611 4631 3817
Size: 794 20 5487
Fragments arranged by size:
5487 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6301
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2929 2964 529
Size: 35 2365 35 3866
Fragments arranged by size:
3866 2365 35 35
DraI TTT'AAA
Cuts at: 2355 3605 2355
Size: 1250 5051
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5308 809
Size: 507 3992 1802
Fragments arranged by size:
3992 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3748 4637 6266
Size: 54 1425 50 275 18 1918 889 1629
Cuts at: 6266 8
Size: 43
Fragments arranged by size:
1918 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3748 1537
Size: 275 1936 4090
Fragments arranged by size:
4090 1936 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 4231 1353
Size: 699 2179 3423
Fragments arranged by size:
3423 2179 699
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4888 5262 5408 387
Size: 1064 138 3299 374 146 1280
Fragments arranged by size:
3299 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2401 2608 3280 4364 4961 5748
Size: 393 405 364 207 672 1084 597 787
Cuts at: 5748 6016 1239
Size: 268 1524
Fragments arranged by size:
1524 1084 787 672 597 405 393 364
268 207
EcoNI CCTnn'n_nnAGG
Cuts at: 2406 2406
Size: 6301
EcoRI G'AATT_C
Cuts at: 2574 3078 3162 3498 3610 3988 2574
Size: 504 84 336 112 378 4887
Fragments arranged by size:
4887 504 378 336 112 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3562 3562
Size: 6301
FspI TGC'GCA
Cuts at: 4807 4807
Size: 6301
HaeII r_GCGC'y
Cuts at: 1678 5448 1678
Size: 3770 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2354 2386 2887 2919 4942
Size: 32 135 32 782 32 501 32 2023
Cuts at: 4942 4974 5016 5048 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2023 782 501 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3645 3891 669
Size: 988 281 1707 246 3079
Fragments arranged by size:
3079 1707 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2703 2909 748
Size: 1955 206 4140
Fragments arranged by size:
4140 1955 206
HpaI GTT'AAC
Cuts at: 3891 3891
Size: 6301
KpnI G_GTAC'C
Cuts at: 3626 3626
Size: 6301
MfeI C'AATT_G
Cuts at: 3900 3900
Size: 6301
MluI A'CGCG_T
Cuts at: 6177 6177
Size: 6301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 5415 5599 1305
Size: 786 3324 184 2007
Fragments arranged by size:
3324 2007 786 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4479
Fragments arranged by size:
4479 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2140 2583 2667 2751 2835 3003 3087
Size: 648 974 443 84 84 84 168 84
Cuts at: 3087 3171 3255 3339 3507 4259 4618 4777 518
Size: 84 84 84 168 752 359 159 2042
Fragments arranged by size:
2042 974 752 648 443 359 168 168
159 84 84 84 84 84 84 84
MspA1I CmG'CkG
Cuts at: 1606 3717 4394 5542 5787 1606
Size: 2111 677 1148 245 2120
Fragments arranged by size:
2120 2111 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2332 513
Size: 552 939 328 4482
Fragments arranged by size:
4482 939 552 328
NdeI CA'TA_TG
Cuts at: 387 2711 387
Size: 2324 3977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6301
NotI GC'GGCC_GC
Cuts at: 3748 3748
Size: 6301
NsiI A_TGCA'T
Cuts at: 2748 2748
Size: 6301
NspI r_CATG'y
Cuts at: 1132 1229 3659 5204 1132
Size: 97 2430 1545 2229
Fragments arranged by size:
2430 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6301
PciI A'CATG_T
Cuts at: 5200 5200
Size: 6301
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6301
PmeI GTTT'AAAC
Cuts at: 3605 3605
Size: 6301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4370 4402 5883 5915 4370
Size: 32 1481 32 4756
Fragments arranged by size:
4756 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6301
PsiI TTA'TAA
Cuts at: 3354 3871 3354
Size: 517 5784
PstI C_TGCA'G
Cuts at: 830 3653 830
Size: 2823 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6301
SalI G'TCGA_C
Cuts at: 1936 3643 1936
Size: 1707 4594
SbfI CC_TGCA'GG
Cuts at: 3653 3653
Size: 6301
ScaI AGT'ACT
Cuts at: 4549 6261 4549
Size: 1712 4589
SfcI C'TryA_G
Cuts at: 826 1045 2119 3649 4784 5465 5656 826
Size: 219 1074 1530 1135 681 191 1471
Fragments arranged by size:
1530 1471 1135 1074 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6301
SmaI CCC'GGG
Cuts at: 1872 3628 1872
Size: 1756 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2339 2403 4405 5306 5568 820
Size: 197 926 396 64 2002 901 262 1553
Fragments arranged by size:
2002 1553 926 901 396 262 197 64
SnaBI TAC'GTA
Cuts at: 493 4092 493
Size: 3599 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6301
SphI G_CATG'C
Cuts at: 1132 3659 1132
Size: 2527 3774
SspI AAT'ATT
Cuts at: 5 52 4225 5
Size: 47 4173 2081
Fragments arranged by size:
4173 2081 47
StuI AGG'CCT
Cuts at: 1517 2957 3041 3293 3377 3461 3545 1517
Size: 1440 84 252 84 84 84 4273
Fragments arranged by size:
4273 1440 252 84 84 84 84
StyI C'CwwG_G
Cuts at: 513 1065 2004 2195 2332 3740 513
Size: 552 939 191 137 1408 3074
Fragments arranged by size:
3074 1408 939 552 191 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4328 4345 4498 4683 5128 325
Size: 1255 2748 17 153 185 445 1498
Fragments arranged by size:
2748 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2094 2731 2983
Size: 274 80 33 51 1443 117 637 252
Cuts at: 2983 4547 6259 96
Size: 1564 1712 138
Fragments arranged by size:
1712 1564 1443 637 274 252 138 117
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6261 6293 6261
Size: 32 6269
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6301
XbaI T'CTAG_A
Cuts at: 3637 3637
Size: 6301
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6301
XmaI C'CCGG_G
Cuts at: 1870 3626 1870
Size: 1756 4545
XmnI GAAnn'nnTTC
Cuts at: 2537 3293 3502 4430 2537
Size: 756 209 928 4408
Fragments arranged by size:
4408 928 756 209
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4109 276
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI