Restriction map for
pFN21AA1938
(Circular) (Six-base) MAPSORT of: pFN21AA1938.seq Check: 6668 from: 1 to: 8734
pFN21AA1938 8734 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6544 278
Size: 53 83 186 936 5008 2468
Fragments arranged by size:
5008 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2784 3423 3582 6077 1937
Size: 847 639 159 2495 4594
Fragments arranged by size:
4594 2495 847 639 159
Acc65I G'GTAC_C
Cuts at: 3090 6055 3090
Size: 2965 5769
AclI AA'CG_TT
Cuts at: 1563 6861 7234 1563
Size: 5298 373 3063
Fragments arranged by size:
5298 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2050 3719 4151 4295 5411 6797 8181 8449
Size: 418 1669 432 144 1116 1386 1384 268
Cuts at: 8449 1632
Size: 1917
Fragments arranged by size:
1917 1669 1386 1384 1116 432 418 268
144
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8537
AflIII A'CryG_T
Cuts at: 1203 5634 7633 8610 1203
Size: 4431 1999 977 1327
Fragments arranged by size:
4431 1999 1327 977
AgeI A'CCGG_T
Cuts at: 3426 7565 3426
Size: 4139 4595
AhdI GACnn_n'nnGTC
Cuts at: 2258 4312 7463 2258
Size: 2054 3151 3529
Fragments arranged by size:
3529 3151 2054
AleI CACnn'nnGTG
Cuts at: 1166 4905 1166
Size: 3739 4995
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2261 5819 1184
Size: 1077 3558 4099
Fragments arranged by size:
4099 3558 1077
AlwNI CAG_nnn'CTG
Cuts at: 2482 3901 4765 5150 5456 6477 8049 2482
Size: 1419 864 385 306 1021 1572 3167
Fragments arranged by size:
3167 1572 1419 1021 864 385 306
ApaI G_GGCC'C
Cuts at: 2518 2518
Size: 8734
ApaLI G'TGCA_C
Cuts at: 2630 3351 3595 6791 7947 2630
Size: 721 244 3196 1156 3417
Fragments arranged by size:
3417 3196 1156 721 244
ApoI r'AATT_y
Cuts at: 1493 3539 6043 6269 6421 8530 1493
Size: 2046 2504 226 152 2109 1697
Fragments arranged by size:
2504 2109 2046 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7288 160
Size: 872 6256 1606
Fragments arranged by size:
6256 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8734
AvaI C'yCGr_G
Cuts at: 1870 1943 2016 2158 2317 2817 3157 3688 4326
Size: 73 73 142 159 500 340 531 638
Cuts at: 4326 6059 1870
Size: 1733 4545
Fragments arranged by size:
4545 1733 638 531 500 340 159 142
73 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2833 2866 2833
Size: 33 8701
BamHI G'GATC_C
Cuts at: 6064 6431 6064
Size: 367 8367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2363 3069 3090 6055 7510
Size: 325 217 627 576 706 21 2965 1455
Cuts at: 7510 618
Size: 1842
Fragments arranged by size:
2965 1842 1455 706 627 576 325 217
21
BanII G_rGCy'C
Cuts at: 1391 2518 2598 2856 3158 3315 4071 4917 6053
Size: 1127 80 258 302 157 756 846 1136
Cuts at: 6053 1391
Size: 4072
Fragments arranged by size:
4072 1136 1127 846 756 302 258 157
80
BbeI G_GCGC'C
Cuts at: 3073 3073
Size: 8734
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2914 928
Size: 971 1015 6748
Fragments arranged by size:
6748 1015 971
BbvCI CC'TCA_GC
Cuts at: 2583 4233 4721 5330 2583
Size: 1650 488 609 5987
Fragments arranged by size:
5987 1650 609 488
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6925 6959 1903
Size: 34 4988 34 3678
Fragments arranged by size:
4988 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6626 7836 6626
Size: 1210 7524
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2383 3062 7345
Size: 107 122 71 631 605 711 679 4283
Cuts at: 7345 136
Size: 1525
Fragments arranged by size:
4283 1525 711 679 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4002 8729 4002
Size: 4727 4007
BlpI GC'TnA_GC
Cuts at: 4112 5847 6151 4112
Size: 1735 304 6695
Fragments arranged by size:
6695 1735 304
Bme1580I G_kGCm'C
Cuts at: 1790 2368 2518 2634 2971 3355 3599 5518 6795
Size: 578 150 116 337 384 244 1919 1277
Cuts at: 6795 7951 1790
Size: 1156 2573
Fragments arranged by size:
2573 1919 1277 1156 578 384 337 244
150 116
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8734
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3813 6465 7423
Size: 416 53 83 399 24 2396 2652 958
Cuts at: 7423 442
Size: 1753
Fragments arranged by size:
2652 2396 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8734
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2031 2563 4984 5629 2031
Size: 532 2421 645 5136
Fragments arranged by size:
5136 2421 645 532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3983 5060 5172 5747 7394 1239
Size: 2744 1077 112 575 1647 2579
Fragments arranged by size:
2744 2579 1647 1077 575 112
Bpu10I CC'TnA_GC
Cuts at: 2402 2583 3169 4233 4721 5330 5747 5886 2402
Size: 181 586 1064 488 609 417 139 5250
Fragments arranged by size:
5250 1064 609 586 488 417 181 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3506 4603 5528 6859 7724 8022 3506
Size: 1097 925 1331 865 298 4218
Fragments arranged by size:
4218 1331 1097 925 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2019 5482 7397 882
Size: 617 520 3463 1915 2219
Fragments arranged by size:
3463 2219 1915 617 520
BsaAI yAC'GTr
Cuts at: 493 1204 6525 493
Size: 711 5321 2702
Fragments arranged by size:
5321 2702 711
BsaBI GATnn'nnATC
Cuts at: 8728 8728
Size: 8734
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3070 4672 6541 6923
Size: 53 83 186 936 1537 1602 1869 382
Cuts at: 6923 7558 275
Size: 635 1451
Fragments arranged by size:
1869 1602 1537 1451 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2217 2457 3426 5643 7167 7565 7839 7986 8397
Size: 240 969 2217 1524 398 274 147 411
Cuts at: 8397 2217
Size: 2554
Fragments arranged by size:
2554 2217 1524 969 411 398 274 240
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2314 2344 1178
Size: 30 165 30 911 30 7568
Fragments arranged by size:
7568 911 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2236 2335 3353 4145 5615 5618 5828 5831
Size: 1050 99 1018 792 1470 3 210 3
Cuts at: 5831 1186
Size: 4089
Fragments arranged by size:
4089 1470 1050 1018 792 210 99 3
3
BseYI C'CCAG_C
Cuts at: 3065 3802 4107 4135 4758 5965 7937 8433 3065
Size: 737 305 28 623 1207 1972 496 3366
Fragments arranged by size:
3366 1972 1207 737 623 496 305 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2598 3321 4450 4598 5615 1163
Size: 573 862 723 1129 148 1017 4282
Fragments arranged by size:
4282 1129 1017 862 723 573 148
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2715 4599 6184 6945 7973 1540
Size: 275 186 714 1884 1585 761 1028 2301
Fragments arranged by size:
2301 1884 1585 1028 761 714 275 186
BsiHKAI G_wGCw'C
Cuts at: 2598 2634 2856 2937 3355 3447 3553 3599 3621
Size: 36 222 81 418 92 106 46 22
Cuts at: 3621 4071 6795 6880 7951 2598
Size: 450 2724 85 1071 3381
Fragments arranged by size:
3381 2724 1071 450 418 222 106 92
85 81 46 36 22
BsmI GAATG_Cn'
Cuts at: 5413 6245 6338 5413
Size: 832 93 7809
Fragments arranged by size:
7809 832 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2245 2245
Size: 8734
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2368 2518 2598 2634 2856 2937 2971
Size: 399 578 150 80 36 222 81 34
Cuts at: 2971 3158 3315 3355 3447 3553 3599 3621 4071
Size: 187 157 40 92 106 46 22 450
Cuts at: 4071 4917 5518 6053 6795 6880 7951 1391
Size: 846 601 535 742 85 1071 2174
Fragments arranged by size:
2174 1071 846 742 601 578 535 450
399 222 187 157 150 106 92 85
81 80 46 40 36 34 22
BspEI T'CCGG_A
Cuts at: 8397 8397
Size: 8734
BspHI T'CATG_A
Cuts at: 2551 3948 6623 8353 2551
Size: 1397 2675 1730 2932
Fragments arranged by size:
2932 2675 1730 1397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2001 2961 3639 4617 5094 6089 844
Size: 1157 960 678 978 477 995 3489
Fragments arranged by size:
3489 1157 995 978 960 678 477
BsrBI CCG'CTC
Cuts at: 1394 1823 2901 4222 5163 5461 5527 5626 5665
Size: 429 1078 1321 941 298 66 99 39
Cuts at: 5665 6621 1394
Size: 956 3507
Fragments arranged by size:
3507 1321 1078 956 941 429 298 99
66 39
BsrDI GCAATG_nn'
Cuts at: 65 1248 3879 5204 7229 7403 65
Size: 1183 2631 1325 2025 174 1396
Fragments arranged by size:
2631 2025 1396 1325 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2269 2956 3426 5574 7378 7565 1700
Size: 110 459 687 470 2148 1804 187 2869
Fragments arranged by size:
2869 2148 1804 687 470 459 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8734
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8734
BssSI C'ACGA_G
Cuts at: 4468 6794 7806 4468
Size: 2326 1012 5396
Fragments arranged by size:
5396 2326 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4504 5095 794
Size: 3710 591 4433
Fragments arranged by size:
4433 3710 591
BstBI TT'CG_AA
Cuts at: 4586 7538 4586
Size: 2952 5782
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8734
BstXI CCAn_nnnn'nTGG
Cuts at: 4688 4907 4688
Size: 219 8515
BstYI r'GATC_y
Cuts at: 1972 2478 3122 4002 6064 6431 6816 6833 8285
Size: 506 644 880 2062 367 385 17 1452
Cuts at: 8285 8371 8383 8729 1972
Size: 86 12 346 1977
Fragments arranged by size:
2062 1977 1452 880 644 506 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5143 5143
Size: 8734
BtgI C'CryG_G
Cuts at: 513 1065 2287 3243 4837 4855 4942 5315 5841
Size: 552 1222 956 1594 18 87 373 526
Cuts at: 5841 5945 513
Size: 104 3302
Fragments arranged by size:
3302 1594 1222 956 552 526 373 104
87 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2063 3739 487
Size: 683 56 837 1676 5482
Fragments arranged by size:
5482 1676 837 683 56
BtsI GCAGTG_nn'
Cuts at: 2581 3092 3121 3676 4076 4142 4585 6250 7044
Size: 511 29 555 400 66 443 1665 794
Cuts at: 7044 7064 2581
Size: 20 4251
Fragments arranged by size:
4251 1665 794 555 511 443 400 66
29 20
ClaI AT'CG_AT
Cuts at: 1554 5561 1554
Size: 4007 4727
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8699
DraI TTT'AAA
Cuts at: 6038 6038
Size: 8734
DraIII CAC_nnn'GTG
Cuts at: 3682 3682
Size: 8734
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3507 7741 809
Size: 507 2191 4234 1802
Fragments arranged by size:
4234 2191 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2712 3054 3408
Size: 54 1425 50 275 18 882 342 354
Cuts at: 3408 3903 4561 4596 4834 4852 5258 6181 7070
Size: 495 658 35 238 18 406 923 889
Cuts at: 7070 8699 8
Size: 1629 43
Fragments arranged by size:
1629 1425 923 889 882 658 495 406
354 342 275 238 54 50 43 35
18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2712 4596 6181 1537
Size: 275 900 1884 1585 4090
Fragments arranged by size:
4090 1884 1585 900 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2321 3483 3788 4802 4975 5526 5649 5735
Size: 968 1162 305 1014 173 551 123 86
Cuts at: 5735 5864 6664 1353
Size: 129 800 3423
Fragments arranged by size:
3423 1162 1014 968 800 551 305 173
129 123 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5388 7321 7695 7841 387
Size: 1064 138 3799 1933 374 146 1280
Fragments arranged by size:
3799 1933 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2596 2854 4069 2596
Size: 258 1215 7261
Fragments arranged by size:
7261 1215 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2050 3719 3983 4151 4295 5060 5172
Size: 393 418 1669 264 168 144 765 112
Cuts at: 5172 5411 5747 6797 7394 8181 8449 1239
Size: 239 336 1050 597 787 268 1524
Fragments arranged by size:
1669 1524 1050 787 765 597 418 393
336 268 264 239 168 144 112
EcoNI CCTnn'n_nnAGG
Cuts at: 2648 3659 4679 5333 2648
Size: 1011 1020 654 6049
Fragments arranged by size:
6049 1020 1011 654
EcoO109I rG'GnC_Cy
Cuts at: 2081 4288 4606 4823 4963 4978 5147 2081
Size: 2207 318 217 140 15 169 5668
Fragments arranged by size:
5668 2207 318 217 169 140 15
EcoRI G'AATT_C
Cuts at: 3539 6043 6421 3539
Size: 2504 378 5852
Fragments arranged by size:
5852 2504 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3952 3952
Size: 8734
FspI TGC'GCA
Cuts at: 3733 7240 3733
Size: 3507 5227
HaeII r_GCGC'y
Cuts at: 1678 3073 3777 7881 1678
Size: 1395 704 4104 2531
Fragments arranged by size:
4104 2531 1395 704
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2010 2042 2244 2276 2470
Size: 32 135 32 438 32 202 32 194
Cuts at: 2470 2502 2722 2754 3528 3560 3994 4026 4298
Size: 32 220 32 774 32 434 32 272
Cuts at: 4298 4330 5698 5730 7375 7407 7449 7481 1373
Size: 32 1368 32 1645 32 42 32 2626
Fragments arranged by size:
2626 1645 1368 774 438 434 272 220
202 194 135 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3670 4516 5232 6078 6324 669
Size: 988 281 1732 846 716 846 246 3079
Fragments arranged by size:
3079 1732 988 846 846 716 281 246
HindIII A'AGCT_T
Cuts at: 748 2530 748
Size: 1782 6952
HpaI GTT'AAC
Cuts at: 6324 6324
Size: 8734
KasI G'GCGC_C
Cuts at: 3069 3069
Size: 8734
KpnI G_GTAC'C
Cuts at: 3094 6059 3094
Size: 2965 5769
MfeI C'AATT_G
Cuts at: 6333 6333
Size: 8734
MluI A'CGCG_T
Cuts at: 8610 8610
Size: 8734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2114 5505 5664 5939 7848 8032 1305
Size: 809 3391 159 275 1909 184 2007
Fragments arranged by size:
3391 2007 1909 809 275 184 159
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3056 3410 3905 4854 10
Size: 54 1425 343 1224 354 495 949 3890
Fragments arranged by size:
3890 1425 1224 949 495 354 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2550 3410 4905 6692 7051 7210 518
Size: 648 1384 860 1495 1787 359 159 2042
Fragments arranged by size:
2042 1787 1495 1384 860 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2170 2582 2746 5175 5268 5358 5367 5854
Size: 564 412 164 2429 93 90 9 487
Cuts at: 5854 6150 6827 7975 8220 1606
Size: 296 677 1148 245 2120
Fragments arranged by size:
2429 2120 1148 677 564 487 412 296
245 164 93 90 9
NaeI GCC'GGC
Cuts at: 2958 5576 2958
Size: 2618 6116
NarI GG'CG_CC
Cuts at: 3070 3070
Size: 8734
NcoI C'CATG_G
Cuts at: 513 1065 3243 4837 4855 4942 5841 513
Size: 552 2178 1594 18 87 899 3406
Fragments arranged by size:
3406 2178 1594 899 552 87 18
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8734
NgoMIV G'CCGG_C
Cuts at: 2956 5574 2956
Size: 2618 6116
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8734
NotI GC'GGCC_GC
Cuts at: 6181 6181
Size: 8734
NspI r_CATG'y
Cuts at: 1132 1229 2872 4085 4717 5098 5488 5638 6092
Size: 97 1643 1213 632 381 390 150 454
Cuts at: 6092 7637 1132
Size: 1545 2229
Fragments arranged by size:
2229 1643 1545 1213 632 454 390 381
150 97
PasI CC'CwG_GG
Cuts at: 1348 2336 4681 4919 1348
Size: 988 2345 238 5163
Fragments arranged by size:
5163 2345 988 238
PciI A'CATG_T
Cuts at: 5634 7633 5634
Size: 1999 6735
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3901 4400 4943 5300 1108
Size: 2793 499 543 357 4542
Fragments arranged by size:
4542 2793 543 499 357
PfoI T'CCnGG_A
Cuts at: 2211 5076 2211
Size: 2865 5869
PmeI GTTT'AAAC
Cuts at: 6038 6038
Size: 8734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6803 6835 8316 8348 6803
Size: 32 1481 32 7189
Fragments arranged by size:
7189 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2081 4288 4606 4823 4963 4978 2081
Size: 2207 318 217 140 15 5837
Fragments arranged by size:
5837 2207 318 217 140 15
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8734
PsiI TTA'TAA
Cuts at: 6304 6304
Size: 8734
PspOMI G'GGCC_C
Cuts at: 2514 2514
Size: 8734
PspXI vC'TCGA_Gb
Cuts at: 2317 4326 2317
Size: 2009 6725
PstI C_TGCA'G
Cuts at: 830 2745 3372 5091 5994 6086 830
Size: 1915 627 1719 903 92 3478
Fragments arranged by size:
3478 1915 1719 903 627 92
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8734
PvuII CAG'CTG
Cuts at: 2170 2582 2170
Size: 412 8322
SacI G_AGCT'C
Cuts at: 2598 2856 4071 2598
Size: 258 1215 7261
Fragments arranged by size:
7261 1215 258
SalI G'TCGA_C
Cuts at: 1936 6076 1936
Size: 4140 4594
SanDI GG'GwC_CC
Cuts at: 2081 4978 2081
Size: 2897 5837
SbfI CC_TGCA'GG
Cuts at: 6086 6086
Size: 8734
ScaI AGT'ACT
Cuts at: 5552 6982 8694 5552
Size: 1430 1712 5592
Fragments arranged by size:
5592 1712 1430
SfcI C'TryA_G
Cuts at: 826 1045 2741 3368 4948 5020 5087 5990 6082
Size: 219 1696 627 1580 72 67 903 92
Cuts at: 6082 7217 7898 8089 826
Size: 1135 681 191 1471
Fragments arranged by size:
1696 1580 1471 1135 903 681 627 219
191 92 72 67
SfoI GGC'GCC
Cuts at: 3071 3071
Size: 8734
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8734
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8734
SmaI CCC'GGG
Cuts at: 1872 2819 6061 1872
Size: 947 3242 4545
Fragments arranged by size:
4545 3242 947
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2016 2317 3521 4326 4618 5543
Size: 197 926 73 301 1204 805 292 925
Cuts at: 5543 6838 7739 8001 820
Size: 1295 901 262 1553
Fragments arranged by size:
1553 1295 1204 926 925 901 805 301
292 262 197 73
SnaBI TAC'GTA
Cuts at: 493 6525 493
Size: 6032 2702
SpeI A'CTAG_T
Cuts at: 152 3355 152
Size: 3203 5531
SphI G_CATG'C
Cuts at: 1132 4085 4717 6092 1132
Size: 2953 632 1375 3774
Fragments arranged by size:
3774 2953 1375 632
SspI AAT'ATT
Cuts at: 5 52 6658 5
Size: 47 6606 2081
Fragments arranged by size:
6606 2081 47
StuI AGG'CCT
Cuts at: 1517 4332 4628 1517
Size: 2815 296 5623
Fragments arranged by size:
5623 2815 296
StyI C'CwwG_G
Cuts at: 513 1065 3243 3387 3885 4374 4644 4837 4855
Size: 552 2178 144 498 489 270 193 18
Cuts at: 4855 4942 5841 5907 6173 513
Size: 87 899 66 266 3074
Fragments arranged by size:
3074 2178 899 552 498 489 270 266
193 144 87 66 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2694 4721 6761 6778 6931 7116 7561
Size: 1255 1114 2027 2040 17 153 185 445
Cuts at: 7561 325
Size: 1498
Fragments arranged by size:
2040 2027 1498 1255 1114 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2349 3374 5535
Size: 274 80 33 51 1443 372 1025 2161
Cuts at: 5535 5550 6980 8692 96
Size: 15 1430 1712 138
Fragments arranged by size:
2161 1712 1443 1430 1025 372 274 138
80 51 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2314 2346 3283 3315 5084 5107 5116 5139 8694
Size: 32 937 32 1769 23 9 23 3555
Cuts at: 8694 8726 2314
Size: 32 2322
Fragments arranged by size:
3555 2322 1769 937 32 32 32 23
23 9
Tth111I GACn'n_nGTC
Cuts at: 1316 2918 4748 1316
Size: 1602 1830 5302
Fragments arranged by size:
5302 1830 1602
XbaI T'CTAG_A
Cuts at: 6070 6070
Size: 8734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4904 5117 5647 4904
Size: 213 530 7991
Fragments arranged by size:
7991 530 213
XhoI C'TCGA_G
Cuts at: 1943 2016 2317 4326 1943
Size: 73 301 2009 6351
Fragments arranged by size:
6351 2009 301 73
XmaI C'CCGG_G
Cuts at: 1870 2817 6059 1870
Size: 947 3242 4545
Fragments arranged by size:
4545 3242 947
XmnI GAAnn'nnTTC
Cuts at: 6863 6863
Size: 8734
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6542 276
Size: 53 83 186 936 5008 2468
Fragments arranged by size:
5008 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BfrBI BsiWI BstZ17I
EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI
RsrII SacII SapI SexAI SfiI SrfI SwaI