Restriction map for
pFN21AA1926
(Circular) (Six-base) MAPSORT of: pFN21AA1926.seq Check: 1951 from: 1 to: 5911
pFN21AA1926 5911 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2158 2158
Size: 5911
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2298 3721 278
Size: 53 83 186 936 762 1423 2468
Fragments arranged by size:
2468 1423 936 762 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3087 3254 1937
Size: 1150 167 4594
Fragments arranged by size:
4594 1150 167
Acc65I G'GTAC_C
Cuts at: 3232 3232
Size: 5911
AclI AA'CG_TT
Cuts at: 1563 4038 4411 1563
Size: 2475 373 3063
Fragments arranged by size:
3063 2475 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2858 3974 5358 5626 1632
Size: 1226 1116 1384 268 1917
Fragments arranged by size:
1917 1384 1226 1116 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5714
AflIII A'CryG_T
Cuts at: 1203 4810 5787 1203
Size: 3607 977 1327
Fragments arranged by size:
3607 1327 977
AgeI A'CCGG_T
Cuts at: 4742 4742
Size: 5911
AhdI GACnn_n'nnGTC
Cuts at: 4640 4640
Size: 5911
AleI CACnn'nnGTG
Cuts at: 1166 2184 2511 1166
Size: 1018 327 4566
Fragments arranged by size:
4566 1018 327
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5911
AlwNI CAG_nnn'CTG
Cuts at: 2081 2162 3654 5226 2081
Size: 81 1492 1572 2766
Fragments arranged by size:
2766 1572 1492 81
ApaLI G'TGCA_C
Cuts at: 2114 3968 5124 2114
Size: 1854 1156 2901
Fragments arranged by size:
2901 1854 1156
ApoI r'AATT_y
Cuts at: 1493 3220 3446 3598 5707 1493
Size: 1727 226 152 2109 1697
Fragments arranged by size:
2109 1727 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4465 160
Size: 872 3433 1606
Fragments arranged by size:
3433 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
AvaI C'yCGr_G
Cuts at: 1870 1943 2016 2682 3236 1870
Size: 73 73 666 554 4545
Fragments arranged by size:
4545 666 554 73 73
BamHI G'GATC_C
Cuts at: 3241 3608 3241
Size: 367 5544
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2189 3121 3232 4687 618
Size: 325 217 627 402 932 111 1455 1842
Fragments arranged by size:
1842 1455 932 627 402 325 217 111
BanII G_rGCy'C
Cuts at: 1391 2141 2691 3230 1391
Size: 750 550 539 4072
Fragments arranged by size:
4072 750 550 539
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2290 928
Size: 971 391 4549
Fragments arranged by size:
4549 971 391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2966 3000 3034 3170 3204 4102 4136
Size: 34 1029 34 34 136 34 898 34
Cuts at: 4136 1903
Size: 3678
Fragments arranged by size:
3678 1029 898 136 34 34 34 34
34
BciVI GTATCCnnnnn_n'
Cuts at: 3803 5013 3803
Size: 1210 4701
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5911
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4522 136
Size: 107 122 71 631 605 2850 1525
Fragments arranged by size:
2850 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5906 5906
Size: 5911
BlpI GC'TnA_GC
Cuts at: 2352 3328 2352
Size: 976 4935
Bme1580I G_kGCm'C
Cuts at: 1790 2118 3972 5128 1790
Size: 328 1854 1156 2573
Fragments arranged by size:
2573 1854 1156 328
BmgBI CAC'GTC
Cuts at: 1318 2808 3208 1318
Size: 1490 400 4021
Fragments arranged by size:
4021 1490 400
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3642 4600 442
Size: 416 53 83 399 24 2225 958 1753
Fragments arranged by size:
2225 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5911
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 5911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 3087 4571 1239
Size: 905 943 1484 2579
Fragments arranged by size:
2579 1484 943 905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2240 4036 4901 5199 2240
Size: 1796 865 298 2952
Fragments arranged by size:
2952 1796 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4574 882
Size: 617 3075 2219
Fragments arranged by size:
3075 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2119 3702 493
Size: 711 915 1583 2702
Fragments arranged by size:
2702 1583 915 711
BsaBI GATnn'nnATC
Cuts at: 5905 5905
Size: 5911
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2061 2247 2295 2793
Size: 53 83 186 936 528 186 48 498
Cuts at: 2793 3063 3084 3718 4100 4735 275
Size: 270 21 634 382 635 1451
Fragments arranged by size:
1451 936 635 634 528 498 382 270
186 186 83 53 48 21
BsaWI w'CCGG_w
Cuts at: 2864 3112 4344 4742 5016 5163 5574 2864
Size: 248 1232 398 274 147 411 3201
Fragments arranged by size:
3201 1232 411 398 274 248 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2117 2147 1178
Size: 30 165 30 714 30 4942
Fragments arranged by size:
4942 714 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5911
BseYI C'CCAG_C
Cuts at: 2316 3132 3138 3144 3162 5114 5610 2316
Size: 816 6 6 18 1952 496 2617
Fragments arranged by size:
2617 1952 816 496 18 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2582 2739 2941 3188 1163
Size: 573 846 157 202 247 3886
Fragments arranged by size:
3886 846 573 247 202 157
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2901 3361 4122 5150 1540
Size: 275 186 900 460 761 1028 2301
Fragments arranged by size:
2301 1028 900 761 460 275 186
BsiHKAI G_wGCw'C
Cuts at: 2118 3112 3972 4057 5128 2118
Size: 994 860 85 1071 2901
Fragments arranged by size:
2901 1071 994 860 85
BsmI GAATG_Cn'
Cuts at: 2327 3422 3515 2327
Size: 1095 93 4723
Fragments arranged by size:
4723 1095 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2141 2691 3112 3230 3972 4057
Size: 399 328 23 550 421 118 742 85
Cuts at: 4057 5128 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 550 421 399 328 118
85 23
BspEI T'CCGG_A
Cuts at: 2864 5574 2864
Size: 2710 3201
BspHI T'CATG_A
Cuts at: 3800 5530 3800
Size: 1730 4181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2158 2823 3266 844
Size: 1314 665 443 3489
Fragments arranged by size:
3489 1314 665 443
BsrBI CCG'CTC
Cuts at: 1394 1823 3798 1394
Size: 429 1975 3507
Fragments arranged by size:
3507 1975 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4406 4580 65
Size: 1183 3158 174 1396
Fragments arranged by size:
3158 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2676 4555 4742 1700
Size: 110 866 1879 187 2869
Fragments arranged by size:
2869 1879 866 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5911
BssHII G'CGCG_C
Cuts at: 1925 2037 2551 2873 2875 2987 3014 3174 3176
Size: 112 514 322 2 112 27 160 2
Cuts at: 3176 1925
Size: 4660
Fragments arranged by size:
4660 514 322 160 112 112 27 2
2
BssSI C'ACGA_G
Cuts at: 3971 4983 3971
Size: 1012 4899
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5911
BstBI TT'CG_AA
Cuts at: 4715 4715
Size: 5911
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5911
BstXI CCAn_nnnn'nTGG
Cuts at: 2186 2449 2513 2186
Size: 263 64 5584
Fragments arranged by size:
5584 263 64
BstYI r'GATC_y
Cuts at: 1972 3241 3608 3993 4010 5462 5548 5560 5906
Size: 1269 367 385 17 1452 86 12 346
Cuts at: 5906 1972
Size: 1977
Fragments arranged by size:
1977 1452 1269 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2049 2182 2233 2341 2512 2901
Size: 552 939 45 133 51 108 171 389
Cuts at: 2901 2961 513
Size: 60 3463
Fragments arranged by size:
3463 939 552 389 171 133 108 60
51 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5172
Fragments arranged by size:
5172 683 56
BtsI GCAGTG_nn'
Cuts at: 2989 3427 4221 4241 2989
Size: 438 794 20 4659
Fragments arranged by size:
4659 794 438 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5911
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2253 2288 529
Size: 35 1689 35 4152
Fragments arranged by size:
4152 1689 35 35
DraI TTT'AAA
Cuts at: 3215 3215
Size: 5911
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4918 809
Size: 507 3602 1802
Fragments arranged by size:
3602 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 2898 2904
Size: 54 1425 50 275 18 268 800 6
Cuts at: 2904 2973 3358 4247 5876 8
Size: 69 385 889 1629 43
Fragments arranged by size:
1629 1425 889 800 385 275 268 69
54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2898 3358 1537
Size: 275 1086 460 4090
Fragments arranged by size:
4090 1086 460 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3083 3841 1353
Size: 1730 758 3423
Fragments arranged by size:
3423 1730 758
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4498 4872 5018 387
Size: 1064 138 2909 374 146 1280
Fragments arranged by size:
2909 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2858 3087 3974 4571 5358 5626
Size: 393 512 714 229 887 597 787 268
Cuts at: 5626 1239
Size: 1524
Fragments arranged by size:
1524 887 787 714 597 512 393 268
229
EcoO109I rG'GnC_Cy
Cuts at: 2312 2746 2312
Size: 434 5477
EcoRI G'AATT_C
Cuts at: 3220 3598 3220
Size: 378 5533
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 5911
FspI TGC'GCA
Cuts at: 2753 3044 4417 2753
Size: 291 1373 4247
Fragments arranged by size:
4247 1373 291
HaeII r_GCGC'y
Cuts at: 1678 2162 2425 5058 1678
Size: 484 263 2633 2531
Fragments arranged by size:
2633 2531 484 263
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 4552 4584 4626
Size: 32 135 32 545 32 2403 32 42
Cuts at: 4626 4658 1373
Size: 32 2626
Fragments arranged by size:
2626 2403 545 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3255 3501 669
Size: 988 281 1317 246 3079
Fragments arranged by size:
3079 1317 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5911
HpaI GTT'AAC
Cuts at: 3501 3501
Size: 5911
KpnI G_GTAC'C
Cuts at: 3236 3236
Size: 5911
MfeI C'AATT_G
Cuts at: 3510 3510
Size: 5911
MluI A'CGCG_T
Cuts at: 5787 5787
Size: 5911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2835 5025 5209 1305
Size: 1530 2190 184 2007
Fragments arranged by size:
2190 2007 1530 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2906 10
Size: 54 1425 343 1074 3015
Fragments arranged by size:
3015 1425 1074 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2184 2511 3869 4228 4387 518
Size: 648 1018 327 1358 359 159 2042
Fragments arranged by size:
2042 1358 1018 648 359 327 159
MspA1I CmG'CkG
Cuts at: 1606 2051 2281 2596 2845 2920 2944 2955 2963
Size: 445 230 315 249 75 24 11 8
Cuts at: 2963 3327 4004 5152 5397 1606
Size: 364 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 445 364 315 249 245
230 75 24 11 8
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4420
Fragments arranged by size:
4420 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5911
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5911
NotI GC'GGCC_GC
Cuts at: 3358 3358
Size: 5911
NspI r_CATG'y
Cuts at: 1132 1229 3269 4814 1132
Size: 97 2040 1545 2229
Fragments arranged by size:
2229 2040 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5911
PciI A'CATG_T
Cuts at: 4810 4810
Size: 5911
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5911
PfoI T'CCnGG_A
Cuts at: 3057 3103 3057
Size: 46 5865
PmeI GTTT'AAAC
Cuts at: 3215 3215
Size: 5911
PmlI CAC'GTG
Cuts at: 2119 2119
Size: 5911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3980 4012 5493 5525 3980
Size: 32 1481 32 4366
Fragments arranged by size:
4366 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5911
PsiI TTA'TAA
Cuts at: 3481 3481
Size: 5911
PstI C_TGCA'G
Cuts at: 830 2361 2590 2943 3263 830
Size: 1531 229 353 320 3478
Fragments arranged by size:
3478 1531 353 320 229
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5911
PvuII CAG'CTG
Cuts at: 2596 2920 2944 2596
Size: 324 24 5563
Fragments arranged by size:
5563 324 24
SacII CC_GC'GG
Cuts at: 2052 2964 2052
Size: 912 4999
SalI G'TCGA_C
Cuts at: 1936 3253 1936
Size: 1317 4594
SapI GCTCTTCn'nnn_
Cuts at: 3083 3083
Size: 5911
SbfI CC_TGCA'GG
Cuts at: 3263 3263
Size: 5911
ScaI AGT'ACT
Cuts at: 4159 5871 4159
Size: 1712 4199
SfcI C'TryA_G
Cuts at: 826 1045 2357 2586 2939 3259 4394 5075 5266
Size: 219 1312 229 353 320 1135 681 191
Cuts at: 5266 826
Size: 1471
Fragments arranged by size:
1471 1312 1135 681 353 320 229 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5911
SmaI CCC'GGG
Cuts at: 1872 2684 3238 1872
Size: 812 554 4545
Fragments arranged by size:
4545 812 554
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2255 4015 4916 5178 820
Size: 197 926 312 1760 901 262 1553
Fragments arranged by size:
1760 1553 926 901 312 262 197
SnaBI TAC'GTA
Cuts at: 493 3702 493
Size: 3209 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5911
SphI G_CATG'C
Cuts at: 1132 3269 1132
Size: 2137 3774
SspI AAT'ATT
Cuts at: 5 52 3835 5
Size: 47 3783 2081
Fragments arranged by size:
3783 2081 47
StuI AGG'CCT
Cuts at: 1517 3034 1517
Size: 1517 4394
StyI C'CwwG_G
Cuts at: 513 1065 2004 2907 3350 513
Size: 552 939 903 443 3074
Fragments arranged by size:
3074 939 903 552 443
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2018 3938 3955 4108 4293 4738 325
Size: 1255 438 1920 17 153 185 445 1498
Fragments arranged by size:
1920 1498 1255 445 438 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4157 5869 96
Size: 274 80 33 51 1443 2180 1712 138
Fragments arranged by size:
2180 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2701 2733 5871 5903 2701
Size: 32 3138 32 2709
Fragments arranged by size:
3138 2709 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2246 1316
Size: 930 4981
XbaI T'CTAG_A
Cuts at: 3247 3247
Size: 5911
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5911
XmaI C'CCGG_G
Cuts at: 1870 2682 3236 1870
Size: 812 554 4545
Fragments arranged by size:
4545 812 554
XmnI GAAnn'nnTTC
Cuts at: 4040 4040
Size: 5911
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2296 3719 276
Size: 53 83 186 936 762 1423 2468
Fragments arranged by size:
2468 1423 936 762 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PstI PvuI PvuII SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII
SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI