Restriction map for
pFN21AA1913
(Circular) (Six-base) MAPSORT of: pFN21AA1913.seq Check: 2176 from: 1 to: 6178
pFN21AA1913 6178 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3988 278
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3521 1937
Size: 1584 4594
Acc65I G'GTAC_C
Cuts at: 3499 3499
Size: 6178
AclI AA'CG_TT
Cuts at: 1563 4305 4678 1563
Size: 2742 373 3063
Fragments arranged by size:
3063 2742 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2952 4241 5625 5893 1632
Size: 1320 1289 1384 268 1917
Fragments arranged by size:
1917 1384 1320 1289 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5981
AflIII A'CryG_T
Cuts at: 1203 5077 6054 1203
Size: 3874 977 1327
Fragments arranged by size:
3874 1327 977
AgeI A'CCGG_T
Cuts at: 5009 5009
Size: 6178
AhdI GACnn_n'nnGTC
Cuts at: 4907 4907
Size: 6178
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2076 1184
Size: 892 5286
AlwNI CAG_nnn'CTG
Cuts at: 3921 5493 3921
Size: 1572 4606
ApaLI G'TGCA_C
Cuts at: 4235 5391 4235
Size: 1156 5022
ApoI r'AATT_y
Cuts at: 1493 2965 3487 3713 3865 5974 1493
Size: 1472 522 226 152 2109 1697
Fragments arranged by size:
2109 1697 1472 522 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4732 160
Size: 872 3700 1606
Fragments arranged by size:
3700 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
AvaI C'yCGr_G
Cuts at: 1870 1943 2136 2611 2644 3503 1870
Size: 73 193 475 33 859 4545
Fragments arranged by size:
4545 859 475 193 73 33
AvrII C'CTAG_G
Cuts at: 3263 3263
Size: 6178
BamHI G'GATC_C
Cuts at: 3508 3875 3508
Size: 367 5811
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2127 3499 4954 618
Size: 325 217 627 340 1372 1455 1842
Fragments arranged by size:
1842 1455 1372 627 340 325 217
BanII G_rGCy'C
Cuts at: 1391 2603 2913 3497 1391
Size: 1212 310 584 4072
Fragments arranged by size:
4072 1212 584 310
BbeI G_GCGC'C
Cuts at: 2131 2131
Size: 6178
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3148 3321 928
Size: 971 1249 173 3785
Fragments arranged by size:
3785 1249 971 173
BbvCI CC'TCA_GC
Cuts at: 2087 2087
Size: 6178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4369 4403 1903
Size: 34 2432 34 3678
Fragments arranged by size:
3678 2432 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4070 5280 4070
Size: 1210 4968
BclI T'GATC_A
Cuts at: 1548 2880 1548
Size: 1332 4846
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2125 4789 136
Size: 107 122 71 631 605 453 2664 1525
Fragments arranged by size:
2664 1525 631 605 453 122 107 71
BglII A'GATC_T
Cuts at: 3398 6173 3398
Size: 2775 3403
BlpI GC'TnA_GC
Cuts at: 3595 3595
Size: 6178
Bme1580I G_kGCm'C
Cuts at: 1790 3342 4239 5395 1790
Size: 1552 897 1156 2573
Fragments arranged by size:
2573 1552 1156 897
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6178
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2907 3909 4867
Size: 416 53 83 399 24 1490 1002 958
Cuts at: 4867 442
Size: 1753
Fragments arranged by size:
1753 1490 1002 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4838 1239
Size: 3599 2579
Bpu10I CC'TnA_GC
Cuts at: 2087 2087
Size: 6178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4303 5168 5466 4303
Size: 865 298 5015
Fragments arranged by size:
5015 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2459 4841 882
Size: 617 960 2382 2219
Fragments arranged by size:
2382 2219 960 617
BsaAI yAC'GTr
Cuts at: 493 1204 3969 493
Size: 711 2765 2702
Fragments arranged by size:
2765 2702 711
BsaBI GATnn'nnATC
Cuts at: 6172 6172
Size: 6178
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2128 3985 4367 5002
Size: 53 83 186 936 595 1857 382 635
Cuts at: 5002 275
Size: 1451
Fragments arranged by size:
1857 1451 936 635 595 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4611 5009 5283 5430 5841 4611
Size: 398 274 147 411 4948
Fragments arranged by size:
4948 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2590 2620 3053 3083 1178
Size: 30 165 30 1187 30 433 30 4273
Fragments arranged by size:
4273 1187 433 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2699 1186
Size: 1513 4665
BseYI C'CCAG_C
Cuts at: 5381 5877 5381
Size: 496 5682
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5605
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2634 3628 4389 5417 1540
Size: 275 186 633 994 761 1028 2301
Fragments arranged by size:
2301 1028 994 761 633 275 186
BsiHKAI G_wGCw'C
Cuts at: 2214 2603 4239 4324 5395 2214
Size: 389 1636 85 1071 2997
Fragments arranged by size:
2997 1636 1071 389 85
BsmI GAATG_Cn'
Cuts at: 2365 3689 3782 2365
Size: 1324 93 4761
Fragments arranged by size:
4761 1324 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2100 2100
Size: 6178
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2603 2913 3342 3497 4239 4324
Size: 399 424 389 310 429 155 742 85
Cuts at: 4324 5395 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 429 424 399 389 310
155 85
BspEI T'CCGG_A
Cuts at: 5841 5841
Size: 6178
BspHI T'CATG_A
Cuts at: 2446 4067 5797 2446
Size: 1621 1730 2827
Fragments arranged by size:
2827 1730 1621
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3533 844
Size: 2689 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4065 1394
Size: 429 2242 3507
Fragments arranged by size:
3507 2242 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2870 4673 4847 65
Size: 1183 1622 1803 174 1396
Fragments arranged by size:
1803 1622 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2130 4822 5009 1700
Size: 110 320 2692 187 2869
Fragments arranged by size:
2869 2692 320 187 110
BsrGI T'GTAC_A
Cuts at: 96 2556 96
Size: 2460 3718
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6178
BssSI C'ACGA_G
Cuts at: 4238 5250 4238
Size: 1012 5166
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2362 2731 794
Size: 1568 369 4241
Fragments arranged by size:
4241 1568 369
BstBI TT'CG_AA
Cuts at: 2665 4982 2665
Size: 2317 3861
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6178
BstYI r'GATC_y
Cuts at: 1972 3398 3508 3875 4260 4277 5729 5815 5827
Size: 1426 110 367 385 17 1452 86 12
Cuts at: 5827 6173 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1426 385 367 346 110 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2400 2679 513
Size: 552 1335 279 4012
Fragments arranged by size:
4012 1335 552 279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5439
Fragments arranged by size:
5439 683 56
BtsI GCAGTG_nn'
Cuts at: 3694 4488 4508 3694
Size: 794 20 5364
Fragments arranged by size:
5364 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2488 2523 529
Size: 35 1924 35 4184
Fragments arranged by size:
4184 1924 35 35
DraI TTT'AAA
Cuts at: 3482 3482
Size: 6178
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5185 809
Size: 507 3869 1802
Fragments arranged by size:
3869 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2238 3625
Size: 54 1425 50 275 18 207 201 1387
Cuts at: 3625 4514 6143 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1387 889 275 207 201 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3625 1537
Size: 275 1813 4090
Fragments arranged by size:
4090 1813 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4108 1353
Size: 2755 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4765 5139 5285 387
Size: 1064 138 3176 374 146 1280
Fragments arranged by size:
3176 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2601 2601
Size: 6178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2952 4241 4838 5625 5893 1239
Size: 393 1320 1289 597 787 268 1524
Fragments arranged by size:
1524 1320 1289 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2047 2047
Size: 6178
EcoO109I rG'GnC_Cy
Cuts at: 3212 3212
Size: 6178
EcoRI G'AATT_C
Cuts at: 3487 3865 3487
Size: 378 5800
EcoRV GAT'ATC
Cuts at: 2491 2491
Size: 6178
FspI TGC'GCA
Cuts at: 4684 4684
Size: 6178
HaeII r_GCGC'y
Cuts at: 1678 2131 5325 1678
Size: 453 3194 2531
Fragments arranged by size:
3194 2531 453
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2199 2231 3053 3085 4819
Size: 32 135 32 627 32 822 32 1734
Cuts at: 4819 4851 4893 4925 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1734 822 627 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2083 2298 3522 3768 669
Size: 988 281 145 215 1224 246 3079
Fragments arranged by size:
3079 1224 988 281 246 215 145
HindIII A'AGCT_T
Cuts at: 748 3380 748
Size: 2632 3546
HpaI GTT'AAC
Cuts at: 3768 3768
Size: 6178
KasI G'GCGC_C
Cuts at: 2127 2127
Size: 6178
KpnI G_GTAC'C
Cuts at: 3503 3503
Size: 6178
MfeI C'AATT_G
Cuts at: 2841 3777 2841
Size: 936 5242
MluI A'CGCG_T
Cuts at: 6054 6054
Size: 6178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3249 5292 5476 1305
Size: 1944 2043 184 2007
Fragments arranged by size:
2043 2007 1944 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4356
Fragments arranged by size:
4356 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2480 4136 4495 4654 518
Size: 648 1314 1656 359 159 2042
Fragments arranged by size:
2042 1656 1314 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2814 3594 4271 5419 5664 1606
Size: 1208 780 677 1148 245 2120
Fragments arranged by size:
2120 1208 1148 780 677 245
NaeI GCC'GGC
Cuts at: 2132 2132
Size: 6178
NarI GG'CG_CC
Cuts at: 2128 2128
Size: 6178
NcoI C'CATG_G
Cuts at: 513 1065 2400 513
Size: 552 1335 4291
Fragments arranged by size:
4291 1335 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6178
NgoMIV G'CCGG_C
Cuts at: 2130 2130
Size: 6178
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6178
NotI GC'GGCC_GC
Cuts at: 3625 3625
Size: 6178
NspI r_CATG'y
Cuts at: 1132 1229 2081 2557 3536 5081 1132
Size: 97 852 476 979 1545 2229
Fragments arranged by size:
2229 1545 979 852 476 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6178
PciI A'CATG_T
Cuts at: 5077 5077
Size: 6178
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6178
PmeI GTTT'AAAC
Cuts at: 3482 3482
Size: 6178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4247 4279 5760 5792 4247
Size: 32 1481 32 4633
Fragments arranged by size:
4633 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3212 3212
Size: 6178
PshAI GACnn'nnGTC
Cuts at: 1613 3210 1613
Size: 1597 4581
PsiI TTA'TAA
Cuts at: 3748 3748
Size: 6178
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2540 2572 2540
Size: 32 6146
PstI C_TGCA'G
Cuts at: 830 3530 830
Size: 2700 3478
PvuI CG_AT'CG
Cuts at: 2001 2634 2001
Size: 633 5545
SacI G_AGCT'C
Cuts at: 2603 2603
Size: 6178
SalI G'TCGA_C
Cuts at: 1936 3520 1936
Size: 1584 4594
SanDI GG'GwC_CC
Cuts at: 3212 3212
Size: 6178
SbfI CC_TGCA'GG
Cuts at: 3530 3530
Size: 6178
ScaI AGT'ACT
Cuts at: 4426 6138 4426
Size: 1712 4466
SfcI C'TryA_G
Cuts at: 826 1045 3526 4661 5342 5533 826
Size: 219 2481 1135 681 191 1471
Fragments arranged by size:
2481 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2129 2129
Size: 6178
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6178
SmaI CCC'GGG
Cuts at: 1872 2138 3505 1872
Size: 266 1367 4545
Fragments arranged by size:
4545 1367 266
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2611 4282 5183 5445 820
Size: 197 926 668 1671 901 262 1553
Fragments arranged by size:
1671 1553 926 901 668 262 197
SnaBI TAC'GTA
Cuts at: 493 3969 493
Size: 3476 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6178
SphI G_CATG'C
Cuts at: 1132 3536 1132
Size: 2404 3774
SspI AAT'ATT
Cuts at: 5 52 4102 5
Size: 47 4050 2081
Fragments arranged by size:
4050 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6178
StyI C'CwwG_G
Cuts at: 513 1065 2400 3041 3195 3263 3617 513
Size: 552 1335 641 154 68 354 3074
Fragments arranged by size:
3074 1335 641 552 354 154 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4205 4222 4375 4560 5005 325
Size: 1255 2625 17 153 185 445 1498
Fragments arranged by size:
2625 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2556 3078 4424
Size: 274 80 33 51 1443 579 522 1346
Cuts at: 4424 6136 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1346 579 522 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2590 2622 3176 3208 6138 6170 2590
Size: 32 554 32 2930 32 2598
Fragments arranged by size:
2930 2598 554 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6178
XbaI T'CTAG_A
Cuts at: 3514 3514
Size: 6178
XhoI C'TCGA_G
Cuts at: 1943 2611 1943
Size: 668 5510
XmaI C'CCGG_G
Cuts at: 1870 2136 3503 1870
Size: 266 1367 4545
Fragments arranged by size:
4545 1367 266
XmnI GAAnn'nnTTC
Cuts at: 4307 4307
Size: 6178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3986 276
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI SacI SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PspOMI PspXI PvuII
RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI