Restriction map for
pFN21AA1903
(Circular) (Six-base) MAPSORT of: pFN21AA1903.seq Check: 3767 from: 1 to: 8101
pFN21AA1903 8101 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3228 5911 278
Size: 53 83 186 936 1692 2683 2468
Fragments arranged by size:
2683 2468 1692 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5444 1937
Size: 3507 4594
Acc65I G'GTAC_C
Cuts at: 5422 5422
Size: 8101
AclI AA'CG_TT
Cuts at: 1563 3303 6228 6601 1563
Size: 1740 2925 373 3063
Fragments arranged by size:
3063 2925 1740 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2094 3623 3873 4383 4488 4517 4770 6164
Size: 462 1529 250 510 105 29 253 1394
Cuts at: 6164 7548 7816 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1529 1394 1384 510 462 268 253
250 105 29
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7904
AflIII A'CryG_T
Cuts at: 1203 2802 3020 3648 4804 7000 7977 1203
Size: 1599 218 628 1156 2196 977 1327
Fragments arranged by size:
2196 1599 1327 1156 977 628 218
AgeI A'CCGG_T
Cuts at: 6932 6932
Size: 8101
AhdI GACnn_n'nnGTC
Cuts at: 4517 6830 4517
Size: 2313 5788
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8101
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8101
AlwNI CAG_nnn'CTG
Cuts at: 2984 3551 5844 7416 2984
Size: 567 2293 1572 3669
Fragments arranged by size:
3669 2293 1572 567
ApaLI G'TGCA_C
Cuts at: 6158 7314 6158
Size: 1156 6945
ApoI r'AATT_y
Cuts at: 1493 2034 2692 2751 3333 3377 4484 4931 5410
Size: 541 658 59 582 44 1107 447 479
Cuts at: 5410 5636 5788 7897 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1107 658 582 541 479 447
226 152 59 44
AseI AT'TA_AT
Cuts at: 160 1032 3755 6655 160
Size: 872 2723 2900 1606
Fragments arranged by size:
2900 2723 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8101
AvaI C'yCGr_G
Cuts at: 1870 1943 5426 1870
Size: 73 3483 4545
Fragments arranged by size:
4545 3483 73
BamHI G'GATC_C
Cuts at: 4793 5431 5798 4793
Size: 638 367 7096
Fragments arranged by size:
7096 638 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4022 5422 6877 618
Size: 325 217 627 2235 1400 1455 1842
Fragments arranged by size:
2235 1842 1455 1400 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2737 5420 1391
Size: 1346 2683 4072
Fragments arranged by size:
4072 2683 1346
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2990 3774 928
Size: 971 1091 784 5255
Fragments arranged by size:
5255 1091 971 784
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6292 6326 1903
Size: 34 4355 34 3678
Fragments arranged by size:
4355 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3877 5993 7203 3877
Size: 2116 1210 4775
Fragments arranged by size:
4775 2116 1210
BclI T'GATC_A
Cuts at: 1548 4864 1548
Size: 3316 4785
BfrBI ATG'CAT
Cuts at: 3999 3999
Size: 8101
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4845 6712 136
Size: 107 122 71 631 605 3173 1867 1525
Fragments arranged by size:
3173 1867 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3491 8096 3491
Size: 4605 3496
BlpI GC'TnA_GC
Cuts at: 5518 5518
Size: 8101
Bme1580I G_kGCm'C
Cuts at: 1790 6162 7318 1790
Size: 4372 1156 2573
Fragments arranged by size:
4372 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8101
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5832 6790 442
Size: 416 53 83 399 24 4415 958 1753
Fragments arranged by size:
4415 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2031 3006 6761 1239
Size: 792 975 3755 2579
Fragments arranged by size:
3755 2579 975 792
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3871 6226 7091 7389 3871
Size: 2355 865 298 4583
Fragments arranged by size:
4583 2355 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6764 882
Size: 617 5265 2219
Fragments arranged by size:
5265 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5892 493
Size: 711 4688 2702
Fragments arranged by size:
4688 2702 711
BsaBI GATnn'nnATC
Cuts at: 4863 8095 4863
Size: 3232 4869
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3225 5908 6290 6925
Size: 53 83 186 936 1692 2683 382 635
Cuts at: 6925 275
Size: 1451
Fragments arranged by size:
2683 1692 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4173 6534 6932 7206 7353 7764 4173
Size: 2361 398 274 147 411 4510
Fragments arranged by size:
4510 2361 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2107 2137 2434 2464 1178
Size: 30 165 30 704 30 297 30 6815
Fragments arranged by size:
6815 704 297 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2146 3090 3741 3744 5100 1186
Size: 960 944 651 3 1356 4187
Fragments arranged by size:
4187 1356 960 944 651 3
BseYI C'CCAG_C
Cuts at: 7304 7800 7304
Size: 496 7605
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2630 1163
Size: 573 894 6634
Fragments arranged by size:
6634 894 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5551 6312 7340 1540
Size: 275 186 3550 761 1028 2301
Fragments arranged by size:
3550 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2737 3276 3414 4424 6162 6247 7318 2737
Size: 539 138 1010 1738 85 1071 3520
Fragments arranged by size:
3520 1738 1071 1010 539 138 85
BsmI GAATG_Cn'
Cuts at: 2103 4616 4760 5612 5705 2103
Size: 2513 144 852 93 4499
Fragments arranged by size:
4499 2513 852 144 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2737 3276 3414 4424 5420 6162 6247
Size: 399 947 539 138 1010 996 742 85
Cuts at: 6247 7318 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1010 996 947 742 539 399
138 85
BspEI T'CCGG_A
Cuts at: 7764 7764
Size: 8101
BspHI T'CATG_A
Cuts at: 5990 7720 5990
Size: 1730 6371
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5456 844
Size: 4612 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3265 5988 1394
Size: 429 1442 2723 3507
Fragments arranged by size:
3507 2723 1442 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6596 6770 65
Size: 1183 5348 174 1396
Fragments arranged by size:
5348 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6745 6932 1700
Size: 110 4935 187 2869
Fragments arranged by size:
4935 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8101
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8101
BssSI C'ACGA_G
Cuts at: 3531 6161 7173 3531
Size: 2630 1012 4459
Fragments arranged by size:
4459 2630 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8101
BstBI TT'CG_AA
Cuts at: 5384 6905 5384
Size: 1521 6580
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8101
BstXI CCAn_nnnn'nTGG
Cuts at: 3402 3402
Size: 8101
BstYI r'GATC_y
Cuts at: 1972 3491 4580 4793 5431 5798 6183 6200 7652
Size: 1519 1089 213 638 367 385 17 1452
Cuts at: 7652 7738 7750 8096 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1519 1452 1089 638 385 367 346
213 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2079 4089 513
Size: 552 1014 2010 4525
Fragments arranged by size:
4525 2010 1014 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4161 487
Size: 683 56 2935 4427
Fragments arranged by size:
4427 2935 683 56
BtsI GCAGTG_nn'
Cuts at: 2642 4407 5617 6411 6431 2642
Size: 1765 1210 794 20 4312
Fragments arranged by size:
4312 1765 1210 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8101
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3644 3679 529
Size: 35 3080 35 4951
Fragments arranged by size:
4951 3080 35 35
DraI TTT'AAA
Cuts at: 5405 5405
Size: 8101
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7108 809
Size: 507 5792 1802
Fragments arranged by size:
5792 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3927 5548 6437
Size: 54 1425 50 275 18 2097 1621 889
Cuts at: 6437 8066 8
Size: 1629 43
Fragments arranged by size:
2097 1629 1621 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5548 1537
Size: 275 3736 4090
Fragments arranged by size:
4090 3736 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3599 4536 4692 5358 6031 1353
Size: 2246 937 156 666 673 3423
Fragments arranged by size:
3423 2246 937 673 666 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6688 7062 7208 387
Size: 1064 138 5099 374 146 1280
Fragments arranged by size:
5099 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2735 2735
Size: 8101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 2094 3006 3623 3873 4383 4488
Size: 393 399 63 912 617 250 510 105
Cuts at: 4488 4517 4770 6164 6761 7548 7816 1239
Size: 29 253 1394 597 787 268 1524
Fragments arranged by size:
1524 1394 912 787 617 597 510 399
393 268 253 250 105 63 29
EcoNI CCTnn'n_nnAGG
Cuts at: 4199 4199
Size: 8101
EcoRI G'AATT_C
Cuts at: 5410 5788 5410
Size: 378 7723
EcoRV GAT'ATC
Cuts at: 2278 3356 2278
Size: 1078 7023
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4547 4547
Size: 8101
FspI TGC'GCA
Cuts at: 6607 6607
Size: 8101
HaeII r_GCGC'y
Cuts at: 1678 7248 1678
Size: 5570 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2538 2570 3725 3757 3994
Size: 32 135 32 966 32 1155 32 237
Cuts at: 3994 4026 4503 4535 6742 6774 6816 6848 1373
Size: 32 477 32 2207 32 42 32 2626
Fragments arranged by size:
2626 2207 1155 966 477 237 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4171 5445 5691 669
Size: 988 281 2233 1274 246 3079
Fragments arranged by size:
3079 2233 1274 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3092 3945 748
Size: 2344 853 4904
Fragments arranged by size:
4904 2344 853
HpaI GTT'AAC
Cuts at: 5691 5691
Size: 8101
KpnI G_GTAC'C
Cuts at: 5426 5426
Size: 8101
MfeI C'AATT_G
Cuts at: 5700 5700
Size: 8101
MluI A'CGCG_T
Cuts at: 7977 7977
Size: 8101
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2394 2693 5093 5291 7215 7399 1305
Size: 1089 299 2400 198 1924 184 2007
Fragments arranged by size:
2400 2007 1924 1089 299 198 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3929 10
Size: 54 1425 343 2097 4182
Fragments arranged by size:
4182 2097 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3249 6059 6418 6577 518
Size: 648 2083 2810 359 159 2042
Fragments arranged by size:
2810 2083 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4019 5517 6194 7342 7587 1606
Size: 2413 1498 677 1148 245 2120
Fragments arranged by size:
2413 2120 1498 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2079 4089 513
Size: 552 1014 2010 4525
Fragments arranged by size:
4525 2010 1014 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8101
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8101
NotI GC'GGCC_GC
Cuts at: 5548 5548
Size: 8101
NsiI A_TGCA'T
Cuts at: 4001 4001
Size: 8101
NspI r_CATG'y
Cuts at: 1132 1229 3024 3652 4808 5126 5140 5459 7004
Size: 97 1795 628 1156 318 14 319 1545
Cuts at: 7004 1132
Size: 2229
Fragments arranged by size:
2229 1795 1545 1156 628 319 318 97
14
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8101
PciI A'CATG_T
Cuts at: 3020 3648 4804 7000 3020
Size: 628 1156 2196 4121
Fragments arranged by size:
4121 2196 1156 628
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8101
PfoI T'CCnGG_A
Cuts at: 5019 5019
Size: 8101
PmeI GTTT'AAAC
Cuts at: 5405 5405
Size: 8101
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4197 4229 6170 6202 7683 7715 4197
Size: 32 1941 32 1481 32 4583
Fragments arranged by size:
4583 1941 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8101
PsiI TTA'TAA
Cuts at: 4293 5671 4293
Size: 1378 6723
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4143 4175 4143
Size: 32 8069
PstI C_TGCA'G
Cuts at: 830 5401 5453 830
Size: 4571 52 3478
Fragments arranged by size:
4571 3478 52
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8101
SacI G_AGCT'C
Cuts at: 2737 2737
Size: 8101
SalI G'TCGA_C
Cuts at: 1936 5443 1936
Size: 3507 4594
SbfI CC_TGCA'GG
Cuts at: 5453 5453
Size: 8101
ScaI AGT'ACT
Cuts at: 2370 2872 6349 8061 2370
Size: 502 3477 1712 2410
Fragments arranged by size:
3477 2410 1712 502
SexAI A'CCwGG_T
Cuts at: 4618 4702 4618
Size: 84 8017
SfcI C'TryA_G
Cuts at: 826 1045 2703 3158 3636 4731 5397 5449 6584
Size: 219 1658 455 478 1095 666 52 1135
Cuts at: 6584 7265 7456 826
Size: 681 191 1471
Fragments arranged by size:
1658 1471 1135 1095 681 666 478 455
219 191 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8101
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8101
SmaI CCC'GGG
Cuts at: 1872 5428 1872
Size: 3556 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3886 6205 7106 7368 820
Size: 197 926 1943 2319 901 262 1553
Fragments arranged by size:
2319 1943 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5892 493
Size: 5399 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8101
SphI G_CATG'C
Cuts at: 1132 5140 5459 1132
Size: 4008 319 3774
Fragments arranged by size:
4008 3774 319
SspI AAT'ATT
Cuts at: 5 52 2346 3085 3111 4441 6025 5
Size: 47 2294 739 26 1330 1584 2081
Fragments arranged by size:
2294 2081 1584 1330 739 47 26
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8101
StyI C'CwwG_G
Cuts at: 513 1065 2079 2557 4089 5540 513
Size: 552 1014 478 1532 1451 3074
Fragments arranged by size:
3074 1532 1451 1014 552 478
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6128 6145 6298 6483 6928 325
Size: 1255 4548 17 153 185 445 1498
Fragments arranged by size:
4548 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2368 2870 3031
Size: 274 80 33 51 1443 391 502 161
Cuts at: 3031 4976 6347 8059 96
Size: 1945 1371 1712 138
Fragments arranged by size:
1945 1712 1443 1371 502 391 274 161
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2107 2139 2964 2996 5149 5181 8061 8093 2107
Size: 32 825 32 2153 32 2880 32 2115
Fragments arranged by size:
2880 2153 2115 825 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8101
XbaI T'CTAG_A
Cuts at: 2569 3974 5437 2569
Size: 1405 1463 5233
Fragments arranged by size:
5233 1463 1405
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4399 4399
Size: 8101
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8101
XmaI C'CCGG_G
Cuts at: 1870 5426 1870
Size: 3556 4545
XmnI GAAnn'nnTTC
Cuts at: 2189 2841 3085 4530 6230 2189
Size: 652 244 1445 1700 4060
Fragments arranged by size:
4060 1700 1445 652 244
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3226 5909 276
Size: 53 83 186 936 1692 2683 2468
Fragments arranged by size:
2683 2468 1692 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PsrI PstI PvuI SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI