Restriction map for
pFN21AA1902
(Circular) (Six-base) MAPSORT of: pFN21AA1902.seq Check: 5446 from: 1 to: 7984
pFN21AA1902 7984 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3030 3030
Size: 7984
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5794 278
Size: 53 83 186 936 4258 2468
Fragments arranged by size:
4258 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3401 5327 1937
Size: 1464 1926 4594
Fragments arranged by size:
4594 1926 1464
Acc65I G'GTAC_C
Cuts at: 5305 5305
Size: 7984
AclI AA'CG_TT
Cuts at: 1563 6111 6484 1563
Size: 4548 373 3063
Fragments arranged by size:
4548 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3929 4526 4805 4838 6047 7431 7699 1632
Size: 2297 597 279 33 1209 1384 268 1917
Fragments arranged by size:
2297 1917 1384 1209 597 279 268 33
AfeI AGC'GCT
Cuts at: 2900 2900
Size: 7984
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7787
AflIII A'CryG_T
Cuts at: 1203 5131 6883 7860 1203
Size: 3928 1752 977 1327
Fragments arranged by size:
3928 1752 1327 977
AgeI A'CCGG_T
Cuts at: 6815 6815
Size: 7984
AhdI GACnn_n'nnGTC
Cuts at: 6713 6713
Size: 7984
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7984
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4412 4851 1184
Size: 3228 439 4317
Fragments arranged by size:
4317 3228 439
AlwNI CAG_nnn'CTG
Cuts at: 3737 4399 4433 5727 7299 3737
Size: 662 34 1294 1572 4422
Fragments arranged by size:
4422 1572 1294 662 34
ApaLI G'TGCA_C
Cuts at: 6041 7197 6041
Size: 1156 6828
ApoI r'AATT_y
Cuts at: 1493 2288 2337 2375 2635 3007 3032 3450 3960
Size: 795 49 38 260 372 25 418 510
Cuts at: 3960 3986 4467 5293 5519 5671 7780 1493
Size: 26 481 826 226 152 2109 1697
Fragments arranged by size:
2109 1697 826 795 510 481 418 372
260 226 152 49 38 26 25
AseI AT'TA_AT
Cuts at: 160 1032 2976 6538 160
Size: 872 1944 3562 1606
Fragments arranged by size:
3562 1944 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7984
AvaI C'yCGr_G
Cuts at: 1870 1943 5309 1870
Size: 73 3366 4545
Fragments arranged by size:
4545 3366 73
BamHI G'GATC_C
Cuts at: 2266 5044 5314 5681 2266
Size: 2778 270 367 4569
Fragments arranged by size:
4569 2778 367 270
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5153 5267 5305 6760 618
Size: 325 217 627 3366 114 38 1455 1842
Fragments arranged by size:
3366 1842 1455 627 325 217 114 38
BanII G_rGCy'C
Cuts at: 1391 2486 3145 4725 5303 1391
Size: 1095 659 1580 578 4072
Fragments arranged by size:
4072 1580 1095 659 578
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2590 2940 3928 4190 4906 5113 928
Size: 971 691 350 988 262 716 207 3799
Fragments arranged by size:
3799 988 971 716 691 350 262 207
BbvCI CC'TCA_GC
Cuts at: 4976 4976
Size: 7984
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6175 6209 1903
Size: 34 4238 34 3678
Fragments arranged by size:
4238 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5876 7086 5876
Size: 1210 6774
BclI T'GATC_A
Cuts at: 1548 2200 5242 1548
Size: 652 3042 4290
Fragments arranged by size:
4290 3042 652
BfrBI ATG'CAT
Cuts at: 4765 4765
Size: 7984
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6595 136
Size: 107 122 71 631 605 4923 1525
Fragments arranged by size:
4923 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4552 5176 7979 4552
Size: 624 2803 4557
Fragments arranged by size:
4557 2803 624
BlpI GC'TnA_GC
Cuts at: 5401 5401
Size: 7984
Bme1580I G_kGCm'C
Cuts at: 1790 6045 7201 1790
Size: 4255 1156 2573
Fragments arranged by size:
4255 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7984
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3907 5715 6673
Size: 416 53 83 399 24 2490 1808 958
Cuts at: 6673 442
Size: 1753
Fragments arranged by size:
2490 1808 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7984
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2527 3986 4493 4709 6644 1239
Size: 878 410 1459 507 216 1935 2579
Fragments arranged by size:
2579 1935 1459 878 507 410 216
Bpu10I CC'TnA_GC
Cuts at: 3766 4976 3766
Size: 1210 6774
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3327 4445 4715 6109 6974 7272 3327
Size: 1118 270 1394 865 298 4039
Fragments arranged by size:
4039 1394 1118 865 298 270
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6647 882
Size: 617 5148 2219
Fragments arranged by size:
5148 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2690 5775 493
Size: 711 1486 3085 2702
Fragments arranged by size:
3085 2702 1486 711
BsaBI GATnn'nnATC
Cuts at: 4302 4355 7978 4302
Size: 53 3623 4308
Fragments arranged by size:
4308 3623 53
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5791 6173 6808 275
Size: 53 83 186 936 4258 382 635 1451
Fragments arranged by size:
4258 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4932 6417 6815 7089 7236 7647 4932
Size: 1485 398 274 147 411 5269
Fragments arranged by size:
5269 1485 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2555 2585 1178
Size: 30 165 30 1152 30 6577
Fragments arranged by size:
6577 1152 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 3579 3675 3684 3687 3711 3714 3750
Size: 928 1465 96 9 3 24 3 36
Cuts at: 3750 4395 4533 5230 1186
Size: 645 138 697 3940
Fragments arranged by size:
3940 1465 928 697 645 138 96 36
24 9 3 3
BseYI C'CCAG_C
Cuts at: 7187 7683 7187
Size: 496 7488
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2513 2577 3771 1163
Size: 573 777 64 1194 5376
Fragments arranged by size:
5376 1194 777 573 64
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5434 6195 7223 1540
Size: 275 186 3433 761 1028 2301
Fragments arranged by size:
3433 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2486 3145 4725 6045 6130 7201 2486
Size: 659 1580 1320 85 1071 3269
Fragments arranged by size:
3269 1580 1320 1071 659 85
BsmI GAATG_Cn'
Cuts at: 3175 3888 4214 4389 5495 5588 3175
Size: 713 326 175 1106 93 5571
Fragments arranged by size:
5571 1106 713 326 175 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2486 3145 4725 5303 6045 6130 7201
Size: 399 696 659 1580 578 742 85 1071
Cuts at: 7201 1391
Size: 2174
Fragments arranged by size:
2174 1580 1071 742 696 659 578 399
85
BspEI T'CCGG_A
Cuts at: 7647 7647
Size: 7984
BspHI T'CATG_A
Cuts at: 2697 3421 4311 5873 7603 2697
Size: 724 890 1562 1730 3078
Fragments arranged by size:
3078 1730 1562 890 724
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2535 2553 3030 3524 4030 5339 844
Size: 1691 18 477 494 506 1309 3489
Fragments arranged by size:
3489 1691 1309 506 494 477 18
BsrBI CCG'CTC
Cuts at: 1394 1823 2577 4267 5871 1394
Size: 429 754 1690 1604 3507
Fragments arranged by size:
3507 1690 1604 754 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2609 6479 6653 65
Size: 1183 1361 3870 174 1396
Fragments arranged by size:
3870 1396 1361 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6628 6815 1700
Size: 110 4818 187 2869
Fragments arranged by size:
4818 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7984
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7984
BssSI C'ACGA_G
Cuts at: 6044 7056 6044
Size: 1012 6972
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2059 3286 4853 794
Size: 1265 1227 1567 3925
Fragments arranged by size:
3925 1567 1265 1227
BstBI TT'CG_AA
Cuts at: 6788 6788
Size: 7984
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7984
BstXI CCAn_nnnn'nTGG
Cuts at: 3265 3265
Size: 7984
BstYI r'GATC_y
Cuts at: 1972 2266 3105 4552 5044 5176 5314 5681 6066
Size: 294 839 1447 492 132 138 367 385
Cuts at: 6066 6083 7535 7621 7633 7979 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1447 839 492 385 367 346
294 138 132 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3571 3571
Size: 7984
BtgI C'CryG_G
Cuts at: 513 1065 2004 3513 513
Size: 552 939 1509 4984
Fragments arranged by size:
4984 1509 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7245
Fragments arranged by size:
7245 683 56
BtsI GCAGTG_nn'
Cuts at: 2594 3599 4187 5500 6294 6314 2594
Size: 1005 588 1313 794 20 4264
Fragments arranged by size:
4264 1313 1005 794 588 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7984
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7949
DraI TTT'AAA
Cuts at: 2875 3975 4537 5288 2875
Size: 1100 562 751 5571
Fragments arranged by size:
5571 1100 751 562
DraIII CAC_nnn'GTG
Cuts at: 2059 4639 2059
Size: 2580 5404
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6991 809
Size: 507 5675 1802
Fragments arranged by size:
5675 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5431 6320 7949
Size: 54 1425 50 275 18 3601 889 1629
Cuts at: 7949 8
Size: 43
Fragments arranged by size:
3601 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5431 1537
Size: 275 3619 4090
Fragments arranged by size:
4090 3619 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4956 5914 1353
Size: 3603 958 3423
Fragments arranged by size:
3603 3423 958
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6571 6945 7091 387
Size: 1064 138 4982 374 146 1280
Fragments arranged by size:
4982 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2484 3143 4723 2484
Size: 659 1580 5745
Fragments arranged by size:
5745 1580 659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2527 3929 3986 4493 4526 4709
Size: 393 485 410 1402 57 507 33 183
Cuts at: 4709 4805 4838 6047 6644 7431 7699 1239
Size: 96 33 1209 597 787 268 1524
Fragments arranged by size:
1524 1402 1209 787 597 507 485 410
393 268 183 96 57 33 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2509 3181 3729 2509
Size: 672 548 6764
Fragments arranged by size:
6764 672 548
EcoO109I rG'GnC_Cy
Cuts at: 2524 3548 3575 3734 4019 2524
Size: 1024 27 159 285 6489
Fragments arranged by size:
6489 1024 285 159 27
EcoRI G'AATT_C
Cuts at: 3960 5293 5671 3960
Size: 1333 378 6273
Fragments arranged by size:
6273 1333 378
EcoRV GAT'ATC
Cuts at: 3475 3475
Size: 7984
FspI TGC'GCA
Cuts at: 6490 6490
Size: 7984
HaeII r_GCGC'y
Cuts at: 1678 2902 5236 7131 1678
Size: 1224 2334 1895 2531
Fragments arranged by size:
2531 2334 1895 1224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2097 2129 2185 2217 4387
Size: 32 135 32 525 32 56 32 2170
Cuts at: 4387 4419 4537 4556 4569 4588 5036 5068 6625
Size: 32 118 19 13 19 448 32 1557
Cuts at: 6625 6657 6699 6731 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2170 1557 525 448 135 118 56
42 32 32 32 32 32 32 32
32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 4575 5328 5574 669
Size: 988 281 2637 753 246 3079
Fragments arranged by size:
3079 2637 988 753 281 246
HindIII A'AGCT_T
Cuts at: 748 3092 748
Size: 2344 5640
HpaI GTT'AAC
Cuts at: 5574 5574
Size: 7984
KpnI G_GTAC'C
Cuts at: 5309 5309
Size: 7984
MfeI C'AATT_G
Cuts at: 5583 5583
Size: 7984
MluI A'CGCG_T
Cuts at: 7860 7860
Size: 7984
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7098 7282 1305
Size: 5793 184 2007
Fragments arranged by size:
5793 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6162
Fragments arranged by size:
6162 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2726 4394 5136 5942 6301 6460 518
Size: 648 1560 1668 742 806 359 159 2042
Fragments arranged by size:
2042 1668 1560 806 742 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2273 3796 3845 5400 6077 7225 7470 1606
Size: 667 1523 49 1555 677 1148 245 2120
Fragments arranged by size:
2120 1555 1523 1148 677 667 245 49
NcoI C'CATG_G
Cuts at: 513 1065 2004 3513 513
Size: 552 939 1509 4984
Fragments arranged by size:
4984 1509 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7984
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7984
NotI GC'GGCC_GC
Cuts at: 5431 5431
Size: 7984
NsiI A_TGCA'T
Cuts at: 4767 4767
Size: 7984
NspI r_CATG'y
Cuts at: 1132 1229 2672 4418 5135 5342 6887 1132
Size: 97 1443 1746 717 207 1545 2229
Fragments arranged by size:
2229 1746 1545 1443 717 207 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7984
PciI A'CATG_T
Cuts at: 5131 6883 5131
Size: 1752 6232
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7984
PmeI GTTT'AAAC
Cuts at: 5288 5288
Size: 7984
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4983 5015 6053 6085 7566 7598 4983
Size: 32 1038 32 1481 32 5369
Fragments arranged by size:
5369 1481 1038 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2524 3548 3575 4019 2524
Size: 1024 27 444 6489
Fragments arranged by size:
6489 1024 444 27
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7984
PsiI TTA'TAA
Cuts at: 4653 5554 4653
Size: 901 7083
PstI C_TGCA'G
Cuts at: 830 3027 4687 4830 5336 830
Size: 2197 1660 143 506 3478
Fragments arranged by size:
3478 2197 1660 506 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7984
PvuII CAG'CTG
Cuts at: 2273 3845 2273
Size: 1572 6412
SacI G_AGCT'C
Cuts at: 2486 3145 4725 2486
Size: 659 1580 5745
Fragments arranged by size:
5745 1580 659
SalI G'TCGA_C
Cuts at: 1936 5326 1936
Size: 3390 4594
SanDI GG'GwC_CC
Cuts at: 3548 3548
Size: 7984
SbfI CC_TGCA'GG
Cuts at: 5336 5336
Size: 7984
ScaI AGT'ACT
Cuts at: 2473 6232 7944 2473
Size: 3759 1712 2513
Fragments arranged by size:
3759 2513 1712
SfcI C'TryA_G
Cuts at: 826 1045 3023 4683 4826 5332 6467 7148 7339
Size: 219 1978 1660 143 506 1135 681 191
Cuts at: 7339 826
Size: 1471
Fragments arranged by size:
1978 1660 1471 1135 681 506 219 191
143
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7984
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7984
SmaI CCC'GGG
Cuts at: 1872 5311 1872
Size: 3439 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3342 4460 4694 6088 6989 7251
Size: 197 926 1399 1118 234 1394 901 262
Cuts at: 7251 820
Size: 1553
Fragments arranged by size:
1553 1399 1394 1118 926 901 262 234
197
SnaBI TAC'GTA
Cuts at: 493 5775 493
Size: 5282 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7984
SphI G_CATG'C
Cuts at: 1132 5342 1132
Size: 4210 3774
SspI AAT'ATT
Cuts at: 5 52 2980 3999 5908 5
Size: 47 2928 1019 1909 2081
Fragments arranged by size:
2928 2081 1909 1019 47
StuI AGG'CCT
Cuts at: 1517 3048 1517
Size: 1531 6453
StyI C'CwwG_G
Cuts at: 513 1065 2004 3177 3513 4015 4489 5423 513
Size: 552 939 1173 336 502 474 934 3074
Fragments arranged by size:
3074 1173 939 934 552 502 474 336
SwaI ATTT'AAAT
Cuts at: 3975 3975
Size: 7984
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2054 3829 6011 6028 6181 6366 6811
Size: 1255 474 1775 2182 17 153 185 445
Cuts at: 6811 325
Size: 1498
Fragments arranged by size:
2182 1775 1498 1255 474 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2471 4755 6230
Size: 274 80 33 51 1443 494 2284 1475
Cuts at: 6230 7942 96
Size: 1712 138
Fragments arranged by size:
2284 1712 1475 1443 494 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4592 4624 4625 4657 7944 7976 4592
Size: 32 1 32 3287 32 4600
Fragments arranged by size:
4600 3287 32 32 32 1
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7984
XbaI T'CTAG_A
Cuts at: 5320 5320
Size: 7984
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3046 3894 3046
Size: 848 7136
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7984
XmaI C'CCGG_G
Cuts at: 1870 5309 1870
Size: 3439 4545
XmnI GAAnn'nnTTC
Cuts at: 2288 2587 3387 3808 4389 4790 6113 2288
Size: 299 800 421 581 401 1323 4159
Fragments arranged by size:
4159 1323 800 581 421 401 299
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5792 276
Size: 53 83 186 936 4258 2468
Fragments arranged by size:
4258 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacII SapI SexAI SfiI SfoI SrfI