Restriction map for
pFN21AA1901
(Circular) (Six-base) MAPSORT of: pFN21AA1901.seq Check: 140 from: 1 to: 8479
pFN21AA1901 8479 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6289 278
Size: 53 83 186 936 4753 2468
Fragments arranged by size:
4753 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5822 1937
Size: 3885 4594
Acc65I G'GTAC_C
Cuts at: 5800 5800
Size: 8479
AclI AA'CG_TT
Cuts at: 1563 6606 6979 1563
Size: 5043 373 3063
Fragments arranged by size:
5043 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2663 2871 3792 3848 4020 4074 4454 4866
Size: 1031 208 921 56 172 54 380 412
Cuts at: 4866 5307 6542 7926 8194 1632
Size: 441 1235 1384 268 1917
Fragments arranged by size:
1917 1384 1235 1031 921 441 412 380
268 208 172 56 54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8282
AflIII A'CryG_T
Cuts at: 1203 2356 5153 7378 8355 1203
Size: 1153 2797 2225 977 1327
Fragments arranged by size:
2797 2225 1327 1153 977
AgeI A'CCGG_T
Cuts at: 7310 7310
Size: 8479
AhdI GACnn_n'nnGTC
Cuts at: 2747 4623 7208 2747
Size: 1876 2585 4018
Fragments arranged by size:
4018 2585 1876
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8479
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5736 1184
Size: 4552 3927
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4947 4979 4947
Size: 32 8447
AlwNI CAG_nnn'CTG
Cuts at: 4433 6222 7794 4433
Size: 1789 1572 5118
Fragments arranged by size:
5118 1789 1572
ApaLI G'TGCA_C
Cuts at: 6536 7692 6536
Size: 1156 7323
ApoI r'AATT_y
Cuts at: 1493 2373 2825 3110 3438 3706 4675 4707 5089
Size: 880 452 285 328 268 969 32 382
Cuts at: 5089 5606 5667 5788 6014 6166 8275 1493
Size: 517 61 121 226 152 2109 1697
Fragments arranged by size:
2109 1697 969 880 517 452 382 328
285 268 226 152 121 61 32
AseI AT'TA_AT
Cuts at: 160 1032 7033 160
Size: 872 6001 1606
Fragments arranged by size:
6001 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8479
AvaI C'yCGr_G
Cuts at: 1870 1943 3321 3651 4080 4628 4858 5804 1870
Size: 73 1378 330 429 548 230 946 4545
Fragments arranged by size:
4545 1378 946 548 429 330 230 73
AvrII C'CTAG_G
Cuts at: 2732 2732
Size: 8479
BamHI G'GATC_C
Cuts at: 5809 6176 5809
Size: 367 8112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5800 7255 618
Size: 325 217 627 4013 1455 1842
Fragments arranged by size:
4013 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2569 5798 1391
Size: 1178 3229 4072
Fragments arranged by size:
4072 3229 1178
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3416 5295 928
Size: 971 1517 1879 4112
Fragments arranged by size:
4112 1879 1517 971
BbvCI CC'TCA_GC
Cuts at: 2808 2808
Size: 8479
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3799 3833 6670 6704 1903
Size: 34 1862 34 2837 34 3678
Fragments arranged by size:
3678 2837 1862 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6371 7581 6371
Size: 1210 7269
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7090 136
Size: 107 122 71 631 605 5418 1525
Fragments arranged by size:
5418 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4016 5167 8474 4016
Size: 1151 3307 4021
Fragments arranged by size:
4021 3307 1151
BlpI GC'TnA_GC
Cuts at: 5896 5896
Size: 8479
Bme1580I G_kGCm'C
Cuts at: 1790 5482 6540 7696 1790
Size: 3692 1058 1156 2573
Fragments arranged by size:
3692 2573 1156 1058
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8479
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6210 7168 442
Size: 416 53 83 399 24 4793 958 1753
Fragments arranged by size:
4793 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3584 1056
Size: 2528 5951
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3021 3078 3468 4569 5603 7139 1239
Size: 1782 57 390 1101 1034 1536 2579
Fragments arranged by size:
2579 1782 1536 1101 1034 390 57
Bpu10I CC'TnA_GC
Cuts at: 2808 2808
Size: 8479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2101 4155 4989 6604 7469 7767 2101
Size: 2054 834 1615 865 298 2813
Fragments arranged by size:
2813 2054 1615 865 834 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7142 882
Size: 617 5643 2219
Fragments arranged by size:
5643 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3848 6270 493
Size: 711 2644 2422 2702
Fragments arranged by size:
2702 2644 2422 711
BsaBI GATnn'nnATC
Cuts at: 5460 8473 5460
Size: 3013 5466
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6286 6668 7303 275
Size: 53 83 186 936 4753 382 635 1451
Fragments arranged by size:
4753 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4951 6912 7310 7584 7731 8142 4951
Size: 1961 398 274 147 411 5288
Fragments arranged by size:
5288 1961 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3286 3316 1178
Size: 30 165 30 1883 30 6341
Fragments arranged by size:
6341 1883 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2796 1186
Size: 1610 6869
BseYI C'CCAG_C
Cuts at: 5465 7682 8178 5465
Size: 2217 496 5766
Fragments arranged by size:
5766 2217 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7906
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5929 6690 7718 1540
Size: 275 186 3928 761 1028 2301
Fragments arranged by size:
3928 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6540 6625 7696 6540
Size: 85 1071 7323
Fragments arranged by size:
7323 1071 85
BsmI GAATG_Cn'
Cuts at: 5543 5990 6083 5543
Size: 447 93 7939
Fragments arranged by size:
7939 447 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2569 5482 5798 6540 6625 7696 1391
Size: 399 779 2913 316 742 85 1071 2174
Fragments arranged by size:
2913 2174 1071 779 742 399 316 85
BspEI T'CCGG_A
Cuts at: 8142 8142
Size: 8479
BspHI T'CATG_A
Cuts at: 4225 5641 6368 8098 4225
Size: 1416 727 1730 4606
Fragments arranged by size:
4606 1730 1416 727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3470 5834 844
Size: 2626 2364 3489
Fragments arranged by size:
3489 2626 2364
BsrBI CCG'CTC
Cuts at: 1394 1823 6366 1394
Size: 429 4543 3507
Fragments arranged by size:
4543 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6974 7148 65
Size: 1183 5726 174 1396
Fragments arranged by size:
5726 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7123 7310 1700
Size: 110 5313 187 2869
Fragments arranged by size:
5313 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2332 2359 2594 96
Size: 2236 27 235 5981
Fragments arranged by size:
5981 2236 235 27
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8479
BssSI C'ACGA_G
Cuts at: 6539 7551 6539
Size: 1012 7467
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3851 794
Size: 3057 5422
BstBI TT'CG_AA
Cuts at: 2846 7283 2846
Size: 4437 4042
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8479
BstYI r'GATC_y
Cuts at: 1972 2263 2657 4016 4394 5167 5809 6176 6561
Size: 291 394 1359 378 773 642 367 385
Cuts at: 6561 6578 8030 8116 8128 8474 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1359 773 642 394 385 378
367 346 291 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6988
Fragments arranged by size:
6988 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7740
Fragments arranged by size:
7740 683 56
BtsI GCAGTG_nn'
Cuts at: 3005 3292 5476 5995 6789 6809 3005
Size: 287 2184 519 794 20 4675
Fragments arranged by size:
4675 2184 794 519 287 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3724 3759 529
Size: 35 3160 35 5249
Fragments arranged by size:
5249 3160 35 35
DraI TTT'AAA
Cuts at: 2722 5783 2722
Size: 3061 5418
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7486 809
Size: 507 6170 1802
Fragments arranged by size:
6170 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5926 6815 8444
Size: 54 1425 50 275 18 4096 889 1629
Cuts at: 8444 8
Size: 43
Fragments arranged by size:
4096 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5926 1537
Size: 275 4114 4090
Fragments arranged by size:
4114 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3038 3603 3786 3915 4089 4399 5385 6409
Size: 1685 565 183 129 174 310 986 1024
Cuts at: 6409 1353
Size: 3423
Fragments arranged by size:
3423 1685 1024 986 565 310 183 174
129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7066 7440 7586 387
Size: 1064 138 5477 374 146 1280
Fragments arranged by size:
5477 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2663 2871 3021 3078 3468 3792 3848
Size: 393 1031 208 150 57 390 324 56
Cuts at: 3848 4020 4074 4454 4569 4866 5307 5603 6542
Size: 172 54 380 115 297 441 296 939
Cuts at: 6542 7139 7926 8194 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1031 939 787 597 441 393 390
380 324 297 296 268 208 172 150
115 57 56 54
EcoO109I rG'GnC_Cy
Cuts at: 2499 4385 5273 2499
Size: 1886 888 5705
Fragments arranged by size:
5705 1886 888
EcoRI G'AATT_C
Cuts at: 3438 5089 5788 6166 3438
Size: 1651 699 378 5751
Fragments arranged by size:
5751 1651 699 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2078 2901 3755 5110 5249 2078
Size: 823 854 1355 139 5308
Fragments arranged by size:
5308 1355 854 823 139
FspI TGC'GCA
Cuts at: 6985 6985
Size: 8479
HaeII r_GCGC'y
Cuts at: 1678 3721 7626 1678
Size: 2043 3905 2531
Fragments arranged by size:
3905 2531 2043
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2378 2410 2729 2733 2761
Size: 32 135 32 806 32 319 4 28
Cuts at: 2761 2765 3286 3318 4609 4641 4841 4873 5563
Size: 4 521 32 1291 32 200 32 690
Cuts at: 5563 5595 7120 7152 7194 7226 1373
Size: 32 1525 32 42 32 2626
Fragments arranged by size:
2626 1525 1291 806 690 521 319 200
135 42 32 32 32 32 32 32
32 32 32 28 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2752 4298 4323 5823 6069 669
Size: 988 281 814 1546 25 1500 246 3079
Fragments arranged by size:
3079 1546 1500 988 814 281 246 25
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8479
HpaI GTT'AAC
Cuts at: 4323 6069 4323
Size: 1746 6733
KpnI G_GTAC'C
Cuts at: 5804 5804
Size: 8479
MfeI C'AATT_G
Cuts at: 6078 6078
Size: 8479
MluI A'CGCG_T
Cuts at: 8355 8355
Size: 8479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3177 4448 7593 7777 1305
Size: 1872 1271 3145 184 2007
Fragments arranged by size:
3145 2007 1872 1271 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6657
Fragments arranged by size:
6657 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2240 2361 3342 3840 4230 5646 5718
Size: 648 1074 121 981 498 390 1416 72
Cuts at: 5718 6437 6796 6955 518
Size: 719 359 159 2042
Fragments arranged by size:
2042 1416 1074 981 719 648 498 390
359 159 121 72
MspA1I CmG'CkG
Cuts at: 1606 5895 6572 7720 7965 1606
Size: 4289 677 1148 245 2120
Fragments arranged by size:
4289 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6988
Fragments arranged by size:
6988 939 552
NdeI CA'TA_TG
Cuts at: 387 3666 387
Size: 3279 5200
NheI G'CTAG_C
Cuts at: 1052 3580 1052
Size: 2528 5951
NotI GC'GGCC_GC
Cuts at: 5926 5926
Size: 8479
NspI r_CATG'y
Cuts at: 1132 1229 2360 2609 5157 5837 7382 1132
Size: 97 1131 249 2548 680 1545 2229
Fragments arranged by size:
2548 2229 1545 1131 680 249 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8479
PciI A'CATG_T
Cuts at: 2356 5153 7378 2356
Size: 2797 2225 3457
Fragments arranged by size:
3457 2797 2225
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4972 1108
Size: 3864 4615
PfoI T'CCnGG_A
Cuts at: 3457 3457
Size: 8479
PmeI GTTT'AAAC
Cuts at: 5783 5783
Size: 8479
PmlI CAC'GTG
Cuts at: 3848 3848
Size: 8479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6548 6580 8061 8093 6548
Size: 32 1481 32 6934
Fragments arranged by size:
6934 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2499 4385 5273 2499
Size: 1886 888 5705
Fragments arranged by size:
5705 1886 888
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8479
PsiI TTA'TAA
Cuts at: 6049 6049
Size: 8479
PspXI vC'TCGA_Gb
Cuts at: 3651 3651
Size: 8479
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5202 5234 5202
Size: 32 8447
PstI C_TGCA'G
Cuts at: 830 3481 3621 5334 5831 830
Size: 2651 140 1713 497 3478
Fragments arranged by size:
3478 2651 1713 497 140
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8479
SalI G'TCGA_C
Cuts at: 1936 5821 1936
Size: 3885 4594
SanDI GG'GwC_CC
Cuts at: 2499 2499
Size: 8479
SapI GCTCTTCn'nnn_
Cuts at: 3915 4399 3915
Size: 484 7995
SbfI CC_TGCA'GG
Cuts at: 5831 5831
Size: 8479
ScaI AGT'ACT
Cuts at: 2470 4864 6727 8439 2470
Size: 2394 1863 1712 2510
Fragments arranged by size:
2510 2394 1863 1712
SexAI A'CCwGG_T
Cuts at: 2634 5365 2634
Size: 2731 5748
SfcI C'TryA_G
Cuts at: 826 1045 2024 2073 2472 3297 3477 3585 3617
Size: 219 979 49 399 825 180 108 32
Cuts at: 3617 3701 5330 5827 6962 7643 7834 826
Size: 84 1629 497 1135 681 191 1471
Fragments arranged by size:
1629 1471 1135 979 825 681 497 399
219 191 180 108 84 49 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8479
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8479
SmaI CCC'GGG
Cuts at: 1872 4082 5806 1872
Size: 2210 1724 4545
Fragments arranged by size:
4545 2210 1724
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2116 3321 3651 4170 4858 5004
Size: 197 926 173 1205 330 519 688 146
Cuts at: 5004 6583 7484 7746 820
Size: 1579 901 262 1553
Fragments arranged by size:
1579 1553 1205 926 901 688 519 330
262 197 173 146
SnaBI TAC'GTA
Cuts at: 493 6270 493
Size: 5777 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8479
SphI G_CATG'C
Cuts at: 1132 5837 1132
Size: 4705 3774
SspI AAT'ATT
Cuts at: 5 52 2191 5662 5692 6403 5
Size: 47 2139 3471 30 711 2081
Fragments arranged by size:
3471 2139 2081 711 47 30
StuI AGG'CCT
Cuts at: 1517 5336 1517
Size: 3819 4660
StyI C'CwwG_G
Cuts at: 513 1065 2004 2732 5918 513
Size: 552 939 728 3186 3074
Fragments arranged by size:
3186 3074 939 728 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6506 6523 6676 6861 7306 325
Size: 1255 4926 17 153 185 445 1498
Fragments arranged by size:
4926 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2332 2359 2428
Size: 274 80 33 51 1443 355 27 69
Cuts at: 2428 2468 2594 3856 4530 4561 4862 6725 8437
Size: 40 126 1262 674 31 301 1863 1712
Cuts at: 8437 96
Size: 138
Fragments arranged by size:
1863 1712 1443 1262 674 355 301 274
138 126 80 69 51 40 33 31
27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8439 8471 8439
Size: 32 8447
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8479
XbaI T'CTAG_A
Cuts at: 5815 5815
Size: 8479
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4407 4407
Size: 8479
XhoI C'TCGA_G
Cuts at: 1943 3321 3651 4858 1943
Size: 1378 330 1207 5564
Fragments arranged by size:
5564 1378 1207 330
XmaI C'CCGG_G
Cuts at: 1870 4080 5804 1870
Size: 2210 1724 4545
Fragments arranged by size:
4545 2210 1724
XmnI GAAnn'nnTTC
Cuts at: 2796 4140 6608 2796
Size: 1344 2468 4667
Fragments arranged by size:
4667 2468 1344
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6287 276
Size: 53 83 186 936 4753 2468
Fragments arranged by size:
4753 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PspOMI PvuII RsrII SacI SacII SfiI
SfoI SrfI SwaI