Restriction map for
pFN21AA1893
(Circular) (Six-base) MAPSORT of: pFN21AA1893.seq Check: 6524 from: 1 to: 7099
pFN21AA1893 7099 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4909 278
Size: 53 83 186 936 3373 2468
Fragments arranged by size:
3373 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2681 4442 1937
Size: 744 1761 4594
Fragments arranged by size:
4594 1761 744
Acc65I G'GTAC_C
Cuts at: 4420 4420
Size: 7099
AclI AA'CG_TT
Cuts at: 1563 5226 5599 1563
Size: 3663 373 3063
Fragments arranged by size:
3663 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2037 3177 3225 3438 3462 5162 6546 6814
Size: 405 1140 48 213 24 1700 1384 268
Cuts at: 6814 1632
Size: 1917
Fragments arranged by size:
1917 1700 1384 1140 405 268 213 48
24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6902
AflIII A'CryG_T
Cuts at: 1203 5998 6975 1203
Size: 4795 977 1327
Fragments arranged by size:
4795 1327 977
AgeI A'CCGG_T
Cuts at: 5930 5930
Size: 7099
AhdI GACnn_n'nnGTC
Cuts at: 3379 3513 3712 5828 3379
Size: 134 199 2116 4650
Fragments arranged by size:
4650 2116 199 134
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7099
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7099
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3104 3136 3104
Size: 32 7067
AlwNI CAG_nnn'CTG
Cuts at: 2396 2564 3353 3686 3971 4842 6414 2396
Size: 168 789 333 285 871 1572 3081
Fragments arranged by size:
3081 1572 871 789 333 285 168
ApaI G_GGCC'C
Cuts at: 2883 2883
Size: 7099
ApaLI G'TGCA_C
Cuts at: 5156 6312 5156
Size: 1156 5943
ApoI r'AATT_y
Cuts at: 1493 4408 4634 4786 6895 1493
Size: 2915 226 152 2109 1697
Fragments arranged by size:
2915 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5653 160
Size: 872 4621 1606
Fragments arranged by size:
4621 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7099
AvaI C'yCGr_G
Cuts at: 1870 1943 2884 3763 3883 4217 4354 4424 1870
Size: 73 941 879 120 334 137 70 4545
Fragments arranged by size:
4545 941 879 334 137 120 73 70
BamHI G'GATC_C
Cuts at: 2512 2584 2608 2639 2704 2728 3040 3229 3253
Size: 72 24 31 65 24 312 189 24
Cuts at: 3253 3775 3871 4015 4022 4429 4796 2512
Size: 522 96 144 7 407 367 4815
Fragments arranged by size:
4815 522 407 367 312 189 144 96
72 65 31 24 24 24 7
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3757 4223 4420 5875 618
Size: 325 217 627 1970 466 197 1455 1842
Fragments arranged by size:
1970 1842 1455 627 466 325 217 197
BanII G_rGCy'C
Cuts at: 1391 2421 2781 2883 2955 3019 3306 3639 4044
Size: 1030 360 102 72 64 287 333 405
Cuts at: 4044 4418 1391
Size: 374 4072
Fragments arranged by size:
4072 1030 405 374 360 333 287 102
72 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2025 3231 3444 3468 4160 928
Size: 971 126 1206 213 24 692 3867
Fragments arranged by size:
3867 1206 971 692 213 126 24
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4180 4214 4317 4351 5290 5324 1903
Size: 34 2243 34 103 34 939 34 3678
Fragments arranged by size:
3678 2243 939 103 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3010 3175 3367 4991 6201 3010
Size: 165 192 1624 1210 3908
Fragments arranged by size:
3908 1624 1210 192 165
BclI T'GATC_A
Cuts at: 1548 4386 1548
Size: 2838 4261
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5710 136
Size: 107 122 71 631 605 4038 1525
Fragments arranged by size:
4038 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2632 2848 3205 7094 2632
Size: 216 357 3889 2637
Fragments arranged by size:
3889 2637 357 216
BlpI GC'TnA_GC
Cuts at: 3537 4204 4516 3537
Size: 667 312 6120
Fragments arranged by size:
6120 667 312
Bme1580I G_kGCm'C
Cuts at: 1790 2883 2907 3762 5160 6316 1790
Size: 1093 24 855 1398 1156 2573
Fragments arranged by size:
2573 1398 1156 1093 855 24
BmgBI CAC'GTC
Cuts at: 1318 2379 1318
Size: 1061 6038
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2220 4830 5788
Size: 416 53 83 399 24 803 2610 958
Cuts at: 5788 442
Size: 1753
Fragments arranged by size:
2610 1753 958 803 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7099
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2335 2335
Size: 7099
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2018 2653 3370 3723 4159 4169 5759 1239
Size: 779 635 717 353 436 10 1590 2579
Fragments arranged by size:
2579 1590 779 717 635 436 353 10
Bpu10I CC'TnA_GC
Cuts at: 3289 3289
Size: 7099
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5224 6089 6387 5224
Size: 865 298 5936
Fragments arranged by size:
5936 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3736 4218 5762 882
Size: 617 2237 482 1544 2219
Fragments arranged by size:
2237 2219 1544 617 482
BsaAI yAC'GTr
Cuts at: 493 1204 4890 493
Size: 711 3686 2702
Fragments arranged by size:
3686 2702 711
BsaBI GATnn'nnATC
Cuts at: 7093 7093
Size: 7099
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3160 4906 5288 5923
Size: 53 83 186 936 1627 1746 382 635
Cuts at: 5923 275
Size: 1451
Fragments arranged by size:
1746 1627 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5532 5930 6204 6351 6762 5532
Size: 398 274 147 411 5869
Fragments arranged by size:
5869 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2129 2159 2768 2798 3001
Size: 30 165 30 726 30 609 30 203
Cuts at: 3001 3031 3128 3158 4053 4083 1178
Size: 30 97 30 895 30 4194
Fragments arranged by size:
4194 895 726 609 203 165 97 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2789 3136 4159 4162 1186
Size: 1603 347 1023 3 4123
Fragments arranged by size:
4123 1603 1023 347 3
BseYI C'CCAG_C
Cuts at: 2148 2234 6302 6798 2148
Size: 86 4068 496 2449
Fragments arranged by size:
4068 2449 496 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2151 4077 1163
Size: 573 415 1926 4185
Fragments arranged by size:
4185 1926 573 415
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4296 4327 4549 5310 6338 1540
Size: 275 186 2295 31 222 761 1028 2301
Fragments arranged by size:
2301 2295 1028 761 275 222 186 31
BsiHKAI G_wGCw'C
Cuts at: 2669 3290 3306 5160 5245 6316 2669
Size: 621 16 1854 85 1071 3452
Fragments arranged by size:
3452 1854 1071 621 85 16
BsmI GAATG_Cn'
Cuts at: 4610 4703 4610
Size: 93 7006
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2421 2669 2781 2883 2907 2955 3019
Size: 399 631 248 112 102 24 48 64
Cuts at: 3019 3290 3306 3639 3762 4044 4418 5160 5245
Size: 271 16 333 123 282 374 742 85
Cuts at: 5245 6316 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 631 399 374 333 282
271 248 123 112 102 85 64 48
24 16
BspEI T'CCGG_A
Cuts at: 6762 6762
Size: 7099
BspHI T'CATG_A
Cuts at: 4988 6718 4988
Size: 1730 5369
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2303 3503 3575 4454 844
Size: 1459 1200 72 879 3489
Fragments arranged by size:
3489 1459 1200 879 72
BsrBI CCG'CTC
Cuts at: 1394 1823 4986 1394
Size: 429 3163 3507
Fragments arranged by size:
3507 3163 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5594 5768 65
Size: 1183 4346 174 1396
Fragments arranged by size:
4346 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4291 5743 5930 1700
Size: 110 2481 1452 187 2869
Fragments arranged by size:
2869 2481 1452 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7099
BssHII G'CGCG_C
Cuts at: 1925 4270 1925
Size: 2345 4754
BssSI C'ACGA_G
Cuts at: 5159 6171 5159
Size: 1012 6087
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2165 4286 794
Size: 1371 2121 3607
Fragments arranged by size:
3607 2121 1371
BstBI TT'CG_AA
Cuts at: 5903 5903
Size: 7099
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7099
BstXI CCAn_nnnn'nTGG
Cuts at: 3052 3502 3052
Size: 450 6649
BstYI r'GATC_y
Cuts at: 1972 2512 2536 2584 2608 2632 2639 2656 2704
Size: 540 24 48 24 24 7 17 48
Cuts at: 2704 2728 2848 2872 2896 3040 3064 3205 3229
Size: 24 120 24 24 144 24 141 24
Cuts at: 3229 3253 3562 3775 3871 4015 4022 4429 4796
Size: 24 309 213 96 144 7 407 367
Cuts at: 4796 5181 5198 6650 6736 6748 7094 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 540 407 385 367 346 309
213 144 144 141 120 96 86 48
48 24 24 24 24 24 24 24
24 24 17 17 12 7 7
BstZ17I GTA'TAC
Cuts at: 2682 2682
Size: 7099
Bsu36I CC'TnA_GG
Cuts at: 3148 3622 4051 3148
Size: 474 429 6196
Fragments arranged by size:
6196 474 429
BtgI C'CryG_G
Cuts at: 513 1065 2004 4296 4321 513
Size: 552 939 2292 25 3291
Fragments arranged by size:
3291 2292 939 552 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6360
Fragments arranged by size:
6360 683 56
BtsI GCAGTG_nn'
Cuts at: 2010 4240 4615 5409 5429 2010
Size: 2230 375 794 20 3680
Fragments arranged by size:
3680 2230 794 375 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7099
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7064
DraI TTT'AAA
Cuts at: 4403 4403
Size: 7099
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4162 6106 809
Size: 507 2846 1944 1802
Fragments arranged by size:
2846 1944 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3172 4293 4324
Size: 54 1425 50 275 18 1342 1121 31
Cuts at: 4324 4546 5435 7064 8
Size: 222 889 1629 43
Fragments arranged by size:
1629 1425 1342 1121 889 275 222 54
50 43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 4293 4324 4546 1537
Size: 275 2481 31 222 4090
Fragments arranged by size:
4090 2481 275 222 31
EarI CTCTTCn'nnn_
Cuts at: 1353 5029 1353
Size: 3676 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3884 4243 5686 6060 6206 387
Size: 1064 138 2295 359 1443 374 146 1280
Fragments arranged by size:
2295 1443 1280 1064 374 359 146 138
EcoICRI GAG'CTC
Cuts at: 3304 3304
Size: 7099
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2018 2037 2653 3177 3225 3370 3438
Size: 393 386 19 616 524 48 145 68
Cuts at: 3438 3462 3723 4159 4169 5162 5759 6546 6814
Size: 24 261 436 10 993 597 787 268
Cuts at: 6814 1239
Size: 1524
Fragments arranged by size:
1524 993 787 616 597 524 436 393
386 268 261 145 68 48 24 19
10
EcoNI CCTnn'n_nnAGG
Cuts at: 3031 3388 4174 3031
Size: 357 786 5956
Fragments arranged by size:
5956 786 357
EcoO109I rG'GnC_Cy
Cuts at: 2066 2244 2520 2880 3024 3373 3397 3498 3515
Size: 178 276 360 144 349 24 101 17
Cuts at: 3515 3658 3879 4317 4394 2066
Size: 143 221 438 77 4771
Fragments arranged by size:
4771 438 360 349 276 221 178 144
143 101 77 24 17
EcoRI G'AATT_C
Cuts at: 4408 4786 4408
Size: 378 6721
FspI TGC'GCA
Cuts at: 5605 5605
Size: 7099
HaeII r_GCGC'y
Cuts at: 1678 3948 6246 1678
Size: 2270 2298 2531
Fragments arranged by size:
2531 2298 2270
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2337 2369 2384 2408 2416
Size: 32 135 32 765 32 15 24 8
Cuts at: 2416 2440 2456 2488 2504 2536 2624 2656 2696
Size: 24 16 32 16 32 88 32 40
Cuts at: 2696 2720 2728 2752 2912 2944 3001 3033 3049
Size: 24 8 24 160 32 57 32 16
Cuts at: 3049 3056 3081 3088 3128 3160 3197 3221 3229
Size: 7 25 7 40 32 37 24 8
Cuts at: 3229 3253 3293 3317 3325 3349 3365 3389 3397
Size: 24 40 24 8 24 16 24 8
Cuts at: 3397 3421 3482 3499 3514 3531 3554 3586 3698
Size: 24 61 17 15 17 23 32 112
Cuts at: 3698 3730 3767 3799 4007 4039 4055 4087 4103
Size: 32 37 32 208 32 16 32 16
Cuts at: 4103 4135 5740 5772 5814 5846 1373
Size: 32 1605 32 42 32 2626
Fragments arranged by size:
2626 1605 765 208 160 135 112 88
61 57 42 40 40 40 37 37
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 25 24 24 24 24 24 24
24 24 24 24 23 17 17 16
16 16 16 16 16 15 15 8
8 8 8 8 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 4443 4689 669
Size: 988 281 2505 246 3079
Fragments arranged by size:
3079 2505 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7099
HpaI GTT'AAC
Cuts at: 4689 4689
Size: 7099
KpnI G_GTAC'C
Cuts at: 4424 4424
Size: 7099
MfeI C'AATT_G
Cuts at: 4698 4698
Size: 7099
MluI A'CGCG_T
Cuts at: 6975 6975
Size: 7099
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4387 6213 6397 1305
Size: 3082 1826 184 2007
Fragments arranged by size:
3082 2007 1826 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3174 10
Size: 54 1425 343 1342 3935
Fragments arranged by size:
3935 1425 1342 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5057 5416 5575 518
Size: 648 3891 359 159 2042
Fragments arranged by size:
3891 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 4298 4515 5192 6340 6585 1606
Size: 432 2260 217 677 1148 245 2120
Fragments arranged by size:
2260 2120 1148 677 432 245 217
NaeI GCC'GGC
Cuts at: 4293 4293
Size: 7099
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5608
Fragments arranged by size:
5608 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7099
NgoMIV G'CCGG_C
Cuts at: 4291 4291
Size: 7099
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7099
NotI GC'GGCC_GC
Cuts at: 4546 4546
Size: 7099
NspI r_CATG'y
Cuts at: 1132 1229 3051 4457 6002 1132
Size: 97 1822 1406 1545 2229
Fragments arranged by size:
2229 1822 1545 1406 97
PasI CC'CwG_GG
Cuts at: 1348 2644 3787 3876 3932 4075 1348
Size: 1296 1143 89 56 143 4372
Fragments arranged by size:
4372 1296 1143 143 89 56
PciI A'CATG_T
Cuts at: 5998 5998
Size: 7099
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2099 1108
Size: 991 6108
PfoI T'CCnGG_A
Cuts at: 2596 3313 3739 4018 2596
Size: 717 426 279 5677
Fragments arranged by size:
5677 717 426 279
PmeI GTTT'AAAC
Cuts at: 4403 4403
Size: 7099
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5168 5200 6681 6713 5168
Size: 32 1481 32 5554
Fragments arranged by size:
5554 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2066 2520 3024 3373 3397 3498 3515 3658 4317
Size: 454 504 349 24 101 17 143 659
Cuts at: 4317 2066
Size: 4848
Fragments arranged by size:
4848 659 504 454 349 143 101 24
17
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7099
PsiI TTA'TAA
Cuts at: 4669 4669
Size: 7099
PspOMI G'GGCC_C
Cuts at: 2879 2879
Size: 7099
PstI C_TGCA'G
Cuts at: 830 2839 3529 3952 4031 4282 4451 830
Size: 2009 690 423 79 251 169 3478
Fragments arranged by size:
3478 2009 690 423 251 169 79
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7099
PvuII CAG'CTG
Cuts at: 2038 2038
Size: 7099
SacI G_AGCT'C
Cuts at: 3306 3306
Size: 7099
SacII CC_GC'GG
Cuts at: 4299 4299
Size: 7099
SalI G'TCGA_C
Cuts at: 1936 4441 1936
Size: 2505 4594
SanDI GG'GwC_CC
Cuts at: 4317 4317
Size: 7099
SbfI CC_TGCA'GG
Cuts at: 4451 4451
Size: 7099
ScaI AGT'ACT
Cuts at: 5347 7059 5347
Size: 1712 5387
SfcI C'TryA_G
Cuts at: 826 1045 2835 3525 3717 3948 4027 4278 4447
Size: 219 1790 690 192 231 79 251 169
Cuts at: 4447 5582 6263 6454 826
Size: 1135 681 191 1471
Fragments arranged by size:
1790 1471 1135 690 681 251 231 219
192 191 169 79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7099
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7099
SmaI CCC'GGG
Cuts at: 1872 4426 1872
Size: 2554 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5203 6104 6366 820
Size: 197 926 3260 901 262 1553
Fragments arranged by size:
3260 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4890 493
Size: 4397 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7099
SphI G_CATG'C
Cuts at: 1132 4457 1132
Size: 3325 3774
SspI AAT'ATT
Cuts at: 5 52 5023 5
Size: 47 4971 2081
Fragments arranged by size:
4971 2081 47
StuI AGG'CCT
Cuts at: 1517 4001 1517
Size: 2484 4615
StyI C'CwwG_G
Cuts at: 513 1065 2004 2322 2907 3027 3501 4122 4538
Size: 552 939 318 585 120 474 621 416
Cuts at: 4538 513
Size: 3074
Fragments arranged by size:
3074 939 621 585 552 474 416 318
120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5126 5143 5296 5481 5926 325
Size: 1255 3546 17 153 185 445 1498
Fragments arranged by size:
3546 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5345 7057 96
Size: 274 80 33 51 1443 3368 1712 138
Fragments arranged by size:
3368 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3269 3301 4053 4085 7059 7091 3269
Size: 32 752 32 2974 32 3277
Fragments arranged by size:
3277 2974 752 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7099
XbaI T'CTAG_A
Cuts at: 2210 3835 4435 2210
Size: 1625 600 4874
Fragments arranged by size:
4874 1625 600
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2272 2329 2272
Size: 57 7042
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7099
XmaI C'CCGG_G
Cuts at: 1870 4424 1870
Size: 2554 4545
XmnI GAAnn'nnTTC
Cuts at: 5228 5228
Size: 7099
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4907 276
Size: 53 83 186 936 3373 2468
Fragments arranged by size:
3373 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI DraIII EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SapI SexAI SfiI SfoI SrfI SwaI