Restriction map for
pFN21AA1887
(Circular) (Six-base) MAPSORT of: pFN21AA1887.seq Check: 1967 from: 1 to: 7714
pFN21AA1887 7714 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2372 2665 2901 2372
Size: 293 236 7185
Fragments arranged by size:
7185 293 236
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5524 278
Size: 53 83 186 936 3988 2468
Fragments arranged by size:
3988 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4441 5057 1937
Size: 2504 616 4594
Fragments arranged by size:
4594 2504 616
Acc65I G'GTAC_C
Cuts at: 5035 5035
Size: 7714
AclI AA'CG_TT
Cuts at: 1563 5841 6214 1563
Size: 4278 373 3063
Fragments arranged by size:
4278 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2754 2862 3801 3831 4785 5777 7161 7429
Size: 1122 108 939 30 954 992 1384 268
Cuts at: 7429 1632
Size: 1917
Fragments arranged by size:
1917 1384 1122 992 954 939 268 108
30
AfeI AGC'GCT
Cuts at: 2084 2084
Size: 7714
AflII C'TTAA_G
Cuts at: 820 1017 4820 820
Size: 197 3803 3714
Fragments arranged by size:
3803 3714 197
AflIII A'CryG_T
Cuts at: 1203 4688 6613 7590 1203
Size: 3485 1925 977 1327
Fragments arranged by size:
3485 1925 1327 977
AgeI A'CCGG_T
Cuts at: 6545 6545
Size: 7714
AhdI GACnn_n'nnGTC
Cuts at: 6443 6443
Size: 7714
AleI CACnn'nnGTG
Cuts at: 1166 2793 1166
Size: 1627 6087
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7714
AlwNI CAG_nnn'CTG
Cuts at: 2546 3583 4481 4541 5457 7029 2546
Size: 1037 898 60 916 1572 3231
Fragments arranged by size:
3231 1572 1037 916 898 60
ApaI G_GGCC'C
Cuts at: 2051 2430 2913 2926 3178 3902 4806 4969 2051
Size: 379 483 13 252 724 904 163 4796
Fragments arranged by size:
4796 904 724 483 379 252 163 13
ApaLI G'TGCA_C
Cuts at: 5771 6927 5771
Size: 1156 6558
ApoI r'AATT_y
Cuts at: 1493 4033 5023 5249 5401 7510 1493
Size: 2540 990 226 152 2109 1697
Fragments arranged by size:
2540 2109 1697 990 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4608 6268 160
Size: 872 3576 1660 1606
Fragments arranged by size:
3576 1660 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7714
AvaI C'yCGr_G
Cuts at: 1870 1943 2254 2824 3171 4069 4153 4448 4629
Size: 73 311 570 347 898 84 295 181
Cuts at: 4629 4799 4831 4938 4962 5039 1870
Size: 170 32 107 24 77 4545
Fragments arranged by size:
4545 898 570 347 311 295 181 170
107 84 77 73 32 24
BamHI G'GATC_C
Cuts at: 4060 4786 5044 5411 4060
Size: 726 258 367 6363
Fragments arranged by size:
6363 726 367 258
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2716 2947 3052 3366 3425
Size: 325 217 627 929 231 105 314 59
Cuts at: 3425 4226 4825 5035 6490 618
Size: 801 599 210 1455 1842
Fragments arranged by size:
1842 1455 929 801 627 599 325 314
231 217 210 105 59
BanII G_rGCy'C
Cuts at: 1391 2051 2399 2430 2735 2913 2926 3178 3353
Size: 660 348 31 305 178 13 252 175
Cuts at: 3353 3729 3902 4276 4354 4457 4782 4806 4969
Size: 376 173 374 78 103 325 24 163
Cuts at: 4969 5033 1391
Size: 64 4072
Fragments arranged by size:
4072 660 376 374 348 325 305 252
178 175 173 163 103 78 64 31
24 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2130 2868 4242 928
Size: 971 231 738 1374 4400
Fragments arranged by size:
4400 1374 971 738 231
BbvCI CC'TCA_GC
Cuts at: 2168 3058 3410 4462 2168
Size: 890 352 1052 5420
Fragments arranged by size:
5420 1052 890 352
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4439 4473 5905 5939 1903
Size: 34 2502 34 1432 34 3678
Fragments arranged by size:
3678 2502 1432 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5606 6816 5606
Size: 1210 6504
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7714
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3784 4486 6325
Size: 107 122 71 631 605 2112 702 1839
Cuts at: 6325 136
Size: 1525
Fragments arranged by size:
2112 1839 1525 702 631 605 122 107
71
BglII A'GATC_T
Cuts at: 7709 7709
Size: 7714
BlpI GC'TnA_GC
Cuts at: 4776 5131 4776
Size: 355 7359
Bme1580I G_kGCm'C
Cuts at: 1790 2051 2430 2721 2913 2926 2950 3049 3178
Size: 261 379 291 192 13 24 99 129
Cuts at: 3178 3430 3902 4105 4806 4830 4969 5775 6931
Size: 252 472 203 701 24 139 806 1156
Cuts at: 6931 1790
Size: 2573
Fragments arranged by size:
2573 1156 806 701 472 379 291 261
252 203 192 139 129 99 24 24
13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7714
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2573 2698 3452
Size: 416 53 83 399 24 1156 125 754
Cuts at: 3452 3509 3708 4529 4563 5006 5445 6403 442
Size: 57 199 821 34 443 439 958 1753
Fragments arranged by size:
1753 1156 958 821 754 443 439 416
399 199 125 83 57 53 34 24
BmtI G_CTAG'C
Cuts at: 1056 2967 1056
Size: 1911 5803
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2717 3162 3186 2717
Size: 445 24 7245
Fragments arranged by size:
7245 445 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2216 2403 2622 4111 4290 4391 4401 6374
Size: 977 187 219 1489 179 101 10 1973
Cuts at: 6374 1239
Size: 2579
Fragments arranged by size:
2579 1973 1489 977 219 187 179 101
10
Bpu10I CC'TnA_GC
Cuts at: 2168 3058 3410 3573 3723 4211 4462 4504 4542
Size: 890 352 163 150 488 251 42 38
Cuts at: 4542 2168
Size: 5340
Fragments arranged by size:
5340 890 488 352 251 163 150 42
38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5839 6704 7002 5839
Size: 865 298 6551
Fragments arranged by size:
6551 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2351 3969 4189 6377 882
Size: 617 852 1618 220 2188 2219
Fragments arranged by size:
2219 2188 1618 852 617 220
BsaAI yAC'GTr
Cuts at: 493 1204 4691 5505 493
Size: 711 3487 814 2702
Fragments arranged by size:
3487 2702 814 711
BsaBI GATnn'nnATC
Cuts at: 7708 7708
Size: 7714
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5521 5903 6538 275
Size: 53 83 186 936 3988 382 635 1451
Fragments arranged by size:
3988 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6147 6545 6819 6966 7377 6147
Size: 398 274 147 411 6484
Fragments arranged by size:
6484 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2797 2827 3213 3243 4389
Size: 30 165 30 1394 30 386 30 1146
Cuts at: 4389 4419 1178
Size: 30 4473
Fragments arranged by size:
4473 1394 1146 386 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2300 2640 2727 2746 2749 2769 3061
Size: 970 144 340 87 19 3 20 292
Cuts at: 3061 3315 3331 3720 4153 4325 4344 4602 5011
Size: 254 16 389 433 172 19 258 409
Cuts at: 5011 1186
Size: 3889
Fragments arranged by size:
3889 970 433 409 389 340 292 258
254 172 144 87 20 19 19 16
3
BseYI C'CCAG_C
Cuts at: 2042 2264 3017 3250 3357 3467 3566 3857 3908
Size: 222 753 233 107 110 99 291 51
Cuts at: 3908 4083 4222 4237 4280 4925 6917 7413 2042
Size: 175 139 15 43 645 1992 496 2343
Fragments arranged by size:
2343 1992 753 645 496 291 233 222
175 139 110 107 99 51 43 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7141
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5164 5925 6953 1540
Size: 275 186 3163 761 1028 2301
Fragments arranged by size:
3163 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2109 4354 5775 5860 6931 2109
Size: 2245 1421 85 1071 2892
Fragments arranged by size:
2892 2245 1421 1071 85
BsmI GAATG_Cn'
Cuts at: 5225 5318 5225
Size: 93 7621
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2109 2399 2430 2721 2735 2913
Size: 399 261 58 290 31 291 14 178
Cuts at: 2913 2926 2950 3049 3178 3353 3430 3729 3902
Size: 13 24 99 129 175 77 299 173
Cuts at: 3902 4105 4276 4354 4457 4782 4806 4830 4969
Size: 203 171 78 103 325 24 24 139
Cuts at: 4969 5033 5775 5860 6931 1391
Size: 64 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 325 299 291 290
261 203 178 175 173 171 139 129
103 99 85 78 77 64 58 31
24 24 24 14 13
BspEI T'CCGG_A
Cuts at: 7377 7377
Size: 7714
BspHI T'CATG_A
Cuts at: 5603 7333 5603
Size: 1730 5984
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2189 2372 2665 2901 5069 844
Size: 1345 183 293 236 2168 3489
Fragments arranged by size:
3489 2168 1345 293 236 183
BsrBI CCG'CTC
Cuts at: 1394 1823 5601 1394
Size: 429 3778 3507
Fragments arranged by size:
3778 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2023 2632 3665 6209 6383 65
Size: 1183 775 609 1033 2544 174 1396
Fragments arranged by size:
2544 1396 1183 1033 775 609 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2313 4650 6358 6545 1700
Size: 110 503 2337 1708 187 2869
Fragments arranged by size:
2869 2337 1708 503 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7714
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7714
BssSI C'ACGA_G
Cuts at: 5774 6786 5774
Size: 1012 6702
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7714
BstBI TT'CG_AA
Cuts at: 6518 6518
Size: 7714
BstEII G'GTnAC_C
Cuts at: 1184 2248 4241 1184
Size: 1064 1993 4657
Fragments arranged by size:
4657 1993 1064
BstXI CCAn_nnnn'nTGG
Cuts at: 3284 4237 3284
Size: 953 6761
BstYI r'GATC_y
Cuts at: 1972 2705 3923 4060 4410 4786 5044 5411 5796
Size: 733 1218 137 350 376 258 367 385
Cuts at: 5796 5813 7265 7351 7363 7709 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1218 733 385 376 367 350
346 258 137 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3343 3343
Size: 7714
BtgI C'CryG_G
Cuts at: 513 1065 2928 4137 4516 513
Size: 552 1863 1209 379 3711
Fragments arranged by size:
3711 1863 1209 552 379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2187 487
Size: 683 56 961 6014
Fragments arranged by size:
6014 961 683 56
BtsI GCAGTG_nn'
Cuts at: 2032 3421 5230 6024 6044 2032
Size: 1389 1809 794 20 3702
Fragments arranged by size:
3702 1809 1389 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7714
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7679
DraI TTT'AAA
Cuts at: 4032 5018 4032
Size: 986 6728
DraIII CAC_nnn'GTG
Cuts at: 2416 3593 4694 2416
Size: 1177 1101 5436
Fragments arranged by size:
5436 1177 1101
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3165 4535 6721 809
Size: 507 1849 1370 2186 1802
Fragments arranged by size:
2186 1849 1802 1370 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2985 4315
Size: 54 1425 50 275 18 225 930 1330
Cuts at: 4315 4663 4858 4975 5161 6050 7679 8
Size: 348 195 117 186 889 1629 43
Fragments arranged by size:
1629 1425 1330 930 889 348 275 225
195 186 117 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5161 1537
Size: 275 3349 4090
Fragments arranged by size:
4090 3349 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2380 2789 4311 5644 1353
Size: 1027 409 1522 1333 3423
Fragments arranged by size:
3423 1522 1333 1027 409
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2332 6301 6675 6821 387
Size: 1064 138 743 3969 374 146 1280
Fragments arranged by size:
3969 1280 1064 743 374 146 138
EcoICRI GAG'CTC
Cuts at: 4352 4352
Size: 7714
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2216 2403 2622 2754 2862 3801 3831
Size: 393 584 187 219 132 108 939 30
Cuts at: 3831 4111 4290 4391 4401 4785 5777 6374 7161
Size: 280 179 101 10 384 992 597 787
Cuts at: 7161 7429 1239
Size: 268 1524
Fragments arranged by size:
1524 992 939 787 597 584 393 384
280 268 219 187 179 132 108 101
30 10
EcoNI CCTnn'n_nnAGG
Cuts at: 4367 4367
Size: 7714
EcoO109I rG'GnC_Cy
Cuts at: 2047 2048 2178 2426 2427 2483 2543 2909 2910
Size: 1 130 248 1 56 60 366 1
Cuts at: 2910 2922 2923 2939 3039 3143 3175 3580 3621
Size: 12 1 16 100 104 32 405 41
Cuts at: 3621 3749 3785 3899 4158 4177 4719 4803 4816
Size: 128 36 114 259 19 542 84 13
Cuts at: 4816 2047
Size: 4945
Fragments arranged by size:
4945 542 405 366 259 248 130 128
114 104 100 84 60 56 41 36
32 19 16 13 12 1 1 1
1
EcoRI G'AATT_C
Cuts at: 5023 5401 5023
Size: 378 7336
FspI TGC'GCA
Cuts at: 6220 6220
Size: 7714
HaeII r_GCGC'y
Cuts at: 1678 2086 6861 1678
Size: 408 4775 2531
Fragments arranged by size:
4775 2531 408
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2717 2749 2818 2850 4052
Size: 32 135 32 1145 32 69 32 1202
Cuts at: 4052 4084 4659 4691 4932 4964 6355 6387 6429
Size: 32 575 32 241 32 1391 32 42
Cuts at: 6429 6461 1373
Size: 32 2626
Fragments arranged by size:
2626 1391 1202 1145 575 241 135 69
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5058 5304 669
Size: 988 281 3120 246 3079
Fragments arranged by size:
3120 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3289 748
Size: 2541 5173
HpaI GTT'AAC
Cuts at: 5304 5304
Size: 7714
KpnI G_GTAC'C
Cuts at: 5039 5039
Size: 7714
MfeI C'AATT_G
Cuts at: 5313 5313
Size: 7714
MluI A'CGCG_T
Cuts at: 7590 7590
Size: 7714
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4209 6828 7012 1305
Size: 2904 2619 184 2007
Fragments arranged by size:
2904 2619 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 4317 10
Size: 54 1425 343 225 2260 3407
Fragments arranged by size:
3407 2260 1425 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2793 3282 5672 6031 6190 518
Size: 648 1627 489 2390 359 159 2042
Fragments arranged by size:
2390 2042 1627 648 489 359 159
MspA1I CmG'CkG
Cuts at: 1606 2984 3443 3908 4946 5130 5807 6955 7200
Size: 1378 459 465 1038 184 677 1148 245
Cuts at: 7200 1606
Size: 2120
Fragments arranged by size:
2120 1378 1148 1038 677 465 459 245
184
NaeI GCC'GGC
Cuts at: 4652 4652
Size: 7714
NcoI C'CATG_G
Cuts at: 513 1065 4516 513
Size: 552 3451 3711
Fragments arranged by size:
3711 3451 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7714
NgoMIV G'CCGG_C
Cuts at: 4650 4650
Size: 7714
NheI G'CTAG_C
Cuts at: 1052 2963 1052
Size: 1911 5803
NotI GC'GGCC_GC
Cuts at: 5161 5161
Size: 7714
NspI r_CATG'y
Cuts at: 1132 1229 5072 6617 1132
Size: 97 3843 1545 2229
Fragments arranged by size:
3843 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2423 2546 2914 3608 4340 1348
Size: 1075 123 368 694 732 4722
Fragments arranged by size:
4722 1075 732 694 368 123
PciI A'CATG_T
Cuts at: 6613 6613
Size: 7714
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2076 2477 4517 1108
Size: 968 401 2040 4305
Fragments arranged by size:
4305 2040 968 401
PfoI T'CCnGG_A
Cuts at: 2380 3207 2380
Size: 827 6887
PmeI GTTT'AAAC
Cuts at: 5018 5018
Size: 7714
PmlI CAC'GTG
Cuts at: 4691 4691
Size: 7714
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5783 5815 7296 7328 5783
Size: 32 1481 32 6169
Fragments arranged by size:
6169 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2178 2483 3749 4719 4816 2178
Size: 305 1266 970 97 5076
Fragments arranged by size:
5076 1266 970 305 97
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7714
PsiI TTA'TAA
Cuts at: 5284 5284
Size: 7714
PspOMI G'GGCC_C
Cuts at: 2047 2426 2909 2922 3174 3898 4802 4965 2047
Size: 379 483 13 252 724 904 163 4796
Fragments arranged by size:
4796 904 724 483 379 252 163 13
PstI C_TGCA'G
Cuts at: 830 2983 3931 4332 5066 830
Size: 2153 948 401 734 3478
Fragments arranged by size:
3478 2153 948 734 401
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7714
PvuII CAG'CTG
Cuts at: 3443 3908 4946 3443
Size: 465 1038 6211
Fragments arranged by size:
6211 1038 465
SacI G_AGCT'C
Cuts at: 4354 4354
Size: 7714
SalI G'TCGA_C
Cuts at: 1936 5056 1936
Size: 3120 4594
SbfI CC_TGCA'GG
Cuts at: 5066 5066
Size: 7714
ScaI AGT'ACT
Cuts at: 5962 7674 5962
Size: 1712 6002
SexAI A'CCwGG_T
Cuts at: 3751 3751
Size: 7714
SfcI C'TryA_G
Cuts at: 826 1045 2791 2979 3927 4328 4397 4932 5062
Size: 219 1746 188 948 401 69 535 130
Cuts at: 5062 6197 6878 7069 826
Size: 1135 681 191 1471
Fragments arranged by size:
1746 1471 1135 948 681 535 401 219
191 188 130 69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7714
SgrAI Cr'CCGG_yG
Cuts at: 1700 2313 1700
Size: 613 7101
SmaI CCC'GGG
Cuts at: 1872 2256 4155 4631 4801 4833 4964 5041 1872
Size: 384 1899 476 170 32 131 77 4545
Fragments arranged by size:
4545 1899 476 384 170 131 77 32
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4820 5818 6719 6981 820
Size: 197 926 2877 998 901 262 1553
Fragments arranged by size:
2877 1553 998 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5505 493
Size: 5012 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7714
SphI G_CATG'C
Cuts at: 1132 5072 1132
Size: 3940 3774
SspI AAT'ATT
Cuts at: 5 52 5638 5
Size: 47 5586 2081
Fragments arranged by size:
5586 2081 47
StuI AGG'CCT
Cuts at: 1517 2525 4489 1517
Size: 1008 1964 4742
Fragments arranged by size:
4742 1964 1008
StyI C'CwwG_G
Cuts at: 513 1065 2142 2217 2564 2618 2988 4516 4782
Size: 552 1077 75 347 54 370 1528 266
Cuts at: 4782 4917 5153 513
Size: 135 236 3074
Fragments arranged by size:
3074 1528 1077 552 370 347 266 236
135 75 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4214 4229 5741 5758 5911 6096 6541
Size: 1255 2634 15 1512 17 153 185 445
Cuts at: 6541 325
Size: 1498
Fragments arranged by size:
2634 1512 1498 1255 445 185 153 17
15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5960 7672 96
Size: 274 80 33 51 1443 3983 1712 138
Fragments arranged by size:
3983 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2196 2228 2402 2434 2940 2972 3385 3417 4045
Size: 32 174 32 506 32 413 32 628
Cuts at: 4045 4077 4678 4710 7674 7706 2196
Size: 32 601 32 2964 32 2204
Fragments arranged by size:
2964 2204 628 601 506 413 174 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2579 3753 1316
Size: 1263 1174 5277
Fragments arranged by size:
5277 1263 1174
XbaI T'CTAG_A
Cuts at: 5050 5050
Size: 7714
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3683 3683
Size: 7714
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7714
XmaI C'CCGG_G
Cuts at: 1870 2254 4153 4629 4799 4831 4962 5039 1870
Size: 384 1899 476 170 32 131 77 4545
Fragments arranged by size:
4545 1899 476 384 170 131 77 32
XmnI GAAnn'nnTTC
Cuts at: 5843 5843
Size: 7714
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5522 276
Size: 53 83 186 936 3988 2468
Fragments arranged by size:
3988 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PspXI PsrI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI