Restriction map for
pFN21AA1878
(Circular) (Six-base) MAPSORT of: pFN21AA1878.seq Check: 6629 from: 1 to: 6022
pFN21AA1878 6022 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3832 278
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3365 1937
Size: 1428 4594
Acc65I G'GTAC_C
Cuts at: 3343 3343
Size: 6022
AclI AA'CG_TT
Cuts at: 1563 4149 4522 1563
Size: 2586 373 3063
Fragments arranged by size:
3063 2586 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4085 5469 5737 1632
Size: 2453 1384 268 1917
Fragments arranged by size:
2453 1917 1384 268
AfeI AGC'GCT
Cuts at: 2612 2612
Size: 6022
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5825
AflIII A'CryG_T
Cuts at: 1203 4921 5898 1203
Size: 3718 977 1327
Fragments arranged by size:
3718 1327 977
AgeI A'CCGG_T
Cuts at: 4853 4853
Size: 6022
AhdI GACnn_n'nnGTC
Cuts at: 4751 4751
Size: 6022
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6022
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2562 1184
Size: 1378 4644
AlwNI CAG_nnn'CTG
Cuts at: 2264 3765 5337 2264
Size: 1501 1572 2949
Fragments arranged by size:
2949 1572 1501
ApaI G_GGCC'C
Cuts at: 2300 2321 3251 2300
Size: 21 930 5071
Fragments arranged by size:
5071 930 21
ApaLI G'TGCA_C
Cuts at: 4079 5235 4079
Size: 1156 4866
ApoI r'AATT_y
Cuts at: 1493 2915 3331 3557 3709 5818 1493
Size: 1422 416 226 152 2109 1697
Fragments arranged by size:
2109 1697 1422 416 226 152
AscI GG'CGCG_CC
Cuts at: 2839 2839
Size: 6022
AseI AT'TA_AT
Cuts at: 160 1032 4576 160
Size: 872 3544 1606
Fragments arranged by size:
3544 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
AvaI C'yCGr_G
Cuts at: 1870 1943 2122 2244 2320 2402 2576 2640 2822
Size: 73 179 122 76 82 174 64 182
Cuts at: 2822 3250 3347 1870
Size: 428 97 4545
Fragments arranged by size:
4545 428 182 179 174 122 97 82
76 73 64
BamHI G'GATC_C
Cuts at: 2523 3352 3719 2523
Size: 829 367 4826
Fragments arranged by size:
4826 829 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2126 2566 2581 3343 4798
Size: 325 217 627 339 440 15 762 1455
Cuts at: 4798 618
Size: 1842
Fragments arranged by size:
1842 1455 762 627 440 339 325 217
15
BanII G_rGCy'C
Cuts at: 1391 2300 2321 2333 2409 3251 3341 1391
Size: 909 21 12 76 842 90 4072
Fragments arranged by size:
4072 909 842 90 76 21 12
BbeI G_GCGC'C
Cuts at: 2130 2570 2585 2130
Size: 440 15 5567
Fragments arranged by size:
5567 440 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5051
BbvCI CC'TCA_GC
Cuts at: 2634 3166 3309 2634
Size: 532 143 5347
Fragments arranged by size:
5347 532 143
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2216 2250 4213 4247 1903
Size: 34 279 34 1963 34 3678
Fragments arranged by size:
3678 1963 279 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3914 5124 3914
Size: 1210 4812
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6022
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2558 2636 2690
Size: 107 122 71 631 605 886 78 54
Cuts at: 2690 4633 136
Size: 1943 1525
Fragments arranged by size:
1943 1525 886 631 605 122 107 78
71 54
BglII A'GATC_T
Cuts at: 2541 6017 2541
Size: 3476 2546
BlpI GC'TnA_GC
Cuts at: 3439 3439
Size: 6022
Bme1580I G_kGCm'C
Cuts at: 1790 2300 2321 2772 3251 4083 5239 1790
Size: 510 21 451 479 832 1156 2573
Fragments arranged by size:
2573 1156 832 510 479 451 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6022
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2461 2962 3753
Size: 416 53 83 399 24 1044 501 791
Cuts at: 3753 4711 442
Size: 958 1753
Fragments arranged by size:
1753 1044 958 791 501 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4682 1239
Size: 3443 2579
Bpu10I CC'TnA_GC
Cuts at: 2634 3166 3309 2634
Size: 532 143 5347
Fragments arranged by size:
5347 532 143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4147 5012 5310 4147
Size: 865 298 4859
Fragments arranged by size:
4859 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2456 2996 4685 882
Size: 617 957 540 1689 2219
Fragments arranged by size:
2219 1689 957 617 540
BsaAI yAC'GTr
Cuts at: 493 1204 3813 493
Size: 711 2609 2702
Fragments arranged by size:
2702 2609 711
BsaBI GATnn'nnATC
Cuts at: 6016 6016
Size: 6022
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2127 2561 2567 2582
Size: 53 83 186 936 594 434 6 15
Cuts at: 2582 2693 2780 2853 3829 4211 4846 275
Size: 111 87 73 976 382 635 1451
Fragments arranged by size:
1451 976 936 635 594 434 382 186
111 87 83 73 53 15 6
BsaWI w'CCGG_w
Cuts at: 4455 4853 5127 5274 5685 4455
Size: 398 274 147 411 4792
Fragments arranged by size:
4792 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2423 2453 1178
Size: 30 165 30 1020 30 4747
Fragments arranged by size:
4747 1020 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2891 1186
Size: 1705 4317
BseYI C'CCAG_C
Cuts at: 2408 5225 5721 2408
Size: 2817 496 2709
Fragments arranged by size:
2817 2709 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2414 2558 2642 2910 1163
Size: 573 678 144 84 268 4275
Fragments arranged by size:
4275 678 573 268 144 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2305 2420 2597 2685 3472 4233
Size: 275 186 304 115 177 88 787 761
Cuts at: 4233 5261 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 787 761 304 275 186 177
115 88
BsiHKAI G_wGCw'C
Cuts at: 2121 3019 4083 4168 5239 2121
Size: 898 1064 85 1071 2904
Fragments arranged by size:
2904 1071 1064 898 85
BsmI GAATG_Cn'
Cuts at: 3533 3626 3533
Size: 93 5929
BsmBI CGTCTCn'nnnn_
Cuts at: 2201 2201
Size: 6022
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2300 2321 2333 2409 2772 3019
Size: 399 331 179 21 12 76 363 247
Cuts at: 3019 3251 3341 4083 4168 5239 1391
Size: 232 90 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 363 331 247 232
179 90 85 76 21 12
BspEI T'CCGG_A
Cuts at: 5685 5685
Size: 6022
BspHI T'CATG_A
Cuts at: 2998 3911 5641 2998
Size: 913 1730 3379
Fragments arranged by size:
3379 1730 913
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3377 844
Size: 2533 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2310 2669 3909 1394
Size: 429 487 359 1240 3507
Fragments arranged by size:
3507 1240 487 429 359
BsrDI GCAATG_nn'
Cuts at: 65 1248 4517 4691 65
Size: 1183 3269 174 1396
Fragments arranged by size:
3269 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2167 2273 2287 2415 2592 2816 2862
Size: 110 357 106 14 128 177 224 46
Cuts at: 2862 4666 4853 1700
Size: 1804 187 2869
Fragments arranged by size:
2869 1804 357 224 187 177 128 110
106 46 14
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6022
BssHII G'CGCG_C
Cuts at: 1925 2189 2191 2839 1925
Size: 264 2 648 5108
Fragments arranged by size:
5108 648 264 2
BssSI C'ACGA_G
Cuts at: 2023 2771 3184 4082 5094 2023
Size: 748 413 898 1012 2951
Fragments arranged by size:
2951 1012 898 748 413
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6022
BstBI TT'CG_AA
Cuts at: 4826 4826
Size: 6022
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6022
BstYI r'GATC_y
Cuts at: 1972 2523 2541 3352 3719 4104 4121 5573 5659
Size: 551 18 811 367 385 17 1452 86
Cuts at: 5659 5671 6017 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 811 551 385 367 346 86
18 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2352 2506 2519 2790 2844 513
Size: 552 939 348 154 13 271 54 3691
Fragments arranged by size:
3691 939 552 348 271 154 54 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2023 2059 487
Size: 683 56 797 36 4450
Fragments arranged by size:
4450 797 683 56 36
BtsI GCAGTG_nn'
Cuts at: 2898 3538 4332 4352 2898
Size: 640 794 20 4568
Fragments arranged by size:
4568 794 640 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6022
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5987
DraI TTT'AAA
Cuts at: 3326 3326
Size: 6022
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3062 5029 809
Size: 507 1746 1967 1802
Fragments arranged by size:
1967 1802 1746 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2012 2302 2417
Size: 54 1425 50 275 18 182 290 115
Cuts at: 2417 2492 2594 2615 2682 3469 4358 5987 8
Size: 75 102 21 67 787 889 1629 43
Fragments arranged by size:
1629 1425 889 787 290 275 182 115
102 75 67 54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2302 2417 2594 2682 3469 1537
Size: 275 490 115 177 88 787 4090
Fragments arranged by size:
4090 787 490 275 177 115 88
EarI CTCTTCn'nnn_
Cuts at: 1353 3952 1353
Size: 2599 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2679 2717 2864 4609 4983 5129
Size: 1064 138 1090 38 147 1745 374 146
Cuts at: 5129 387
Size: 1280
Fragments arranged by size:
1745 1280 1090 1064 374 147 146 138
38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4085 4682 5469 5737 1239
Size: 393 2453 597 787 268 1524
Fragments arranged by size:
2453 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2028 2297 2761 3247 2028
Size: 269 464 486 4803
Fragments arranged by size:
4803 486 464 269
EcoRI G'AATT_C
Cuts at: 2915 3331 3709 2915
Size: 416 378 5228
Fragments arranged by size:
5228 416 378
FspI TGC'GCA
Cuts at: 4528 4528
Size: 6022
HaeII r_GCGC'y
Cuts at: 1678 2130 2174 2264 2570 2585 2593 2614 5169
Size: 452 44 90 306 15 8 21 2555
Cuts at: 5169 1678
Size: 2531
Fragments arranged by size:
2555 2531 452 306 90 44 21 15
8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4663 4695 4737 4769 1373
Size: 32 135 32 3091 32 42 32 2626
Fragments arranged by size:
3091 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3366 3612 669
Size: 988 281 1428 246 3079
Fragments arranged by size:
3079 1428 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6022
HpaI GTT'AAC
Cuts at: 3612 3612
Size: 6022
KasI G'GCGC_C
Cuts at: 2126 2566 2581 2126
Size: 440 15 5567
Fragments arranged by size:
5567 440 15
KpnI G_GTAC'C
Cuts at: 3347 3347
Size: 6022
MfeI C'AATT_G
Cuts at: 3621 3621
Size: 6022
MluI A'CGCG_T
Cuts at: 5898 5898
Size: 6022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2040 2211 2466 5136 5320 1305
Size: 735 171 255 2670 184 2007
Fragments arranged by size:
2670 2007 735 255 184 171
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2617 10
Size: 54 1425 343 785 3415
Fragments arranged by size:
3415 1425 785 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2078 3980 4339 4498 518
Size: 648 912 1902 359 159 2042
Fragments arranged by size:
2042 1902 912 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2086 2354 2398 2412 2497 2508 2633 2785
Size: 480 268 44 14 85 11 125 152
Cuts at: 2785 2792 2846 3053 3438 4115 5263 5508 1606
Size: 7 54 207 385 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 480 385 268 245 207
152 125 85 54 44 14 11 7
NaeI GCC'GGC
Cuts at: 2169 2289 2417 2594 2169
Size: 120 128 177 5597
Fragments arranged by size:
5597 177 128 120
NarI GG'CG_CC
Cuts at: 2127 2567 2582 2127
Size: 440 15 5567
Fragments arranged by size:
5567 440 15
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4531
Fragments arranged by size:
4531 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6022
NgoMIV G'CCGG_C
Cuts at: 2167 2287 2415 2592 2167
Size: 120 128 177 5597
Fragments arranged by size:
5597 177 128 120
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6022
NotI GC'GGCC_GC
Cuts at: 3469 3469
Size: 6022
NspI r_CATG'y
Cuts at: 1132 1229 3380 4925 1132
Size: 97 2151 1545 2229
Fragments arranged by size:
2229 2151 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2221 1348
Size: 873 5149
PciI A'CATG_T
Cuts at: 4921 4921
Size: 6022
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2264 1108
Size: 902 254 4866
Fragments arranged by size:
4866 902 254
PmeI GTTT'AAAC
Cuts at: 3326 3326
Size: 6022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4091 4123 5604 5636 4091
Size: 32 1481 32 4477
Fragments arranged by size:
4477 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6022
PsiI TTA'TAA
Cuts at: 3592 3592
Size: 6022
PspOMI G'GGCC_C
Cuts at: 2296 2317 3247 2296
Size: 21 930 5071
Fragments arranged by size:
5071 930 21
PstI C_TGCA'G
Cuts at: 830 3374 830
Size: 2544 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6022
PvuII CAG'CTG
Cuts at: 2412 3053 2412
Size: 641 5381
SacII CC_GC'GG
Cuts at: 2355 2509 2793 2847 2355
Size: 154 284 54 5530
Fragments arranged by size:
5530 284 154 54
SalI G'TCGA_C
Cuts at: 1936 3364 1936
Size: 1428 4594
SbfI CC_TGCA'GG
Cuts at: 3374 3374
Size: 6022
ScaI AGT'ACT
Cuts at: 2102 4270 5982 2102
Size: 2168 1712 2142
Fragments arranged by size:
2168 2142 1712
SfcI C'TryA_G
Cuts at: 826 1045 2905 3010 3094 3370 4505 5186 5377
Size: 219 1860 105 84 276 1135 681 191
Cuts at: 5377 826
Size: 1471
Fragments arranged by size:
1860 1471 1135 681 276 219 191 105
84
SfoI GGC'GCC
Cuts at: 2128 2568 2583 2128
Size: 440 15 5567
Fragments arranged by size:
5567 440 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6022
SmaI CCC'GGG
Cuts at: 1872 2124 2246 2322 2642 3349 1872
Size: 252 122 76 320 707 4545
Fragments arranged by size:
4545 707 320 252 122 76
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4126 5027 5289 820
Size: 197 926 2183 901 262 1553
Fragments arranged by size:
2183 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3813 493
Size: 3320 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6022
SphI G_CATG'C
Cuts at: 1132 3380 1132
Size: 2248 3774
SrfI GCCC'GGGC
Cuts at: 2246 2322 2246
Size: 76 5946
SspI AAT'ATT
Cuts at: 5 52 3946 5
Size: 47 3894 2081
Fragments arranged by size:
3894 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6022
StyI C'CwwG_G
Cuts at: 513 1065 2004 3461 513
Size: 552 939 1457 3074
Fragments arranged by size:
3074 1457 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4049 4066 4219 4404 4849 325
Size: 1255 2469 17 153 185 445 1498
Fragments arranged by size:
2469 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2100 2970 4268
Size: 274 80 33 51 1443 123 870 1298
Cuts at: 4268 5980 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1298 870 274 138 123 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2504 2536 5982 6014 2504
Size: 32 3446 32 2512
Fragments arranged by size:
3446 2512 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6022
XbaI T'CTAG_A
Cuts at: 3358 3358
Size: 6022
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6022
XmaI C'CCGG_G
Cuts at: 1870 2122 2244 2320 2640 3347 1870
Size: 252 122 76 320 707 4545
Fragments arranged by size:
4545 707 320 252 122 76
XmnI GAAnn'nnTTC
Cuts at: 2480 2915 4151 2480
Size: 435 1236 4351
Fragments arranged by size:
4351 1236 435
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3830 276
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AvrII BaeI BfrBI BplI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacI SanDI SapI SexAI SfiI SwaI
XcmI