Restriction map for
pFN21AA1877
(Circular) (Six-base) MAPSORT of: pFN21AA1877.seq Check: 2546 from: 1 to: 6292
pFN21AA1877 6292 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3099 4102 278
Size: 53 83 186 936 1563 1003 2468
Fragments arranged by size:
2468 1563 1003 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3635 1937
Size: 1698 4594
Acc65I G'GTAC_C
Cuts at: 3613 3613
Size: 6292
AclI AA'CG_TT
Cuts at: 1563 4419 4792 1563
Size: 2856 373 3063
Fragments arranged by size:
3063 2856 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4355 5739 6007 1632
Size: 2723 1384 268 1917
Fragments arranged by size:
2723 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6095
AflIII A'CryG_T
Cuts at: 1203 2556 2987 3360 5191 6168 1203
Size: 1353 431 373 1831 977 1327
Fragments arranged by size:
1831 1353 1327 977 431 373
AgeI A'CCGG_T
Cuts at: 5123 5123
Size: 6292
AhdI GACnn_n'nnGTC
Cuts at: 5021 5021
Size: 6292
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3371 3579 1184
Size: 2187 208 3897
Fragments arranged by size:
3897 2187 208
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2627 2659 2627
Size: 32 6260
AlwNI CAG_nnn'CTG
Cuts at: 2242 2758 3049 3487 4035 5607 2242
Size: 516 291 438 548 1572 2927
Fragments arranged by size:
2927 1572 548 516 438 291
ApaI G_GGCC'C
Cuts at: 2305 2305
Size: 6292
ApaLI G'TGCA_C
Cuts at: 3356 3554 4349 5505 3356
Size: 198 795 1156 4143
Fragments arranged by size:
4143 1156 795 198
ApoI r'AATT_y
Cuts at: 1493 3239 3601 3827 3979 6088 1493
Size: 1746 362 226 152 2109 1697
Fragments arranged by size:
2109 1746 1697 362 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3036 4846 160
Size: 872 2004 1810 1606
Fragments arranged by size:
2004 1810 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
AvaI C'yCGr_G
Cuts at: 1870 1943 2462 3440 3617 1870
Size: 73 519 978 177 4545
Fragments arranged by size:
4545 978 519 177 73
BamHI G'GATC_C
Cuts at: 3622 3989 3622
Size: 367 5925
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2192 2532 2801 2809 2835
Size: 325 217 627 405 340 269 8 26
Cuts at: 2835 2842 3613 5068 618
Size: 7 771 1455 1842
Fragments arranged by size:
1842 1455 771 627 405 340 325 269
217 26 8 7
BanII G_rGCy'C
Cuts at: 1391 2067 2208 2305 3611 1391
Size: 676 141 97 1306 4072
Fragments arranged by size:
4072 1306 676 141 97
BbeI G_GCGC'C
Cuts at: 2196 2813 2839 2196
Size: 617 26 5649
Fragments arranged by size:
5649 617 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5321
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4483 4517 1903
Size: 34 2546 34 3678
Fragments arranged by size:
3678 2546 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4184 5394 4184
Size: 1210 5082
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6292
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2422 4903 136
Size: 107 122 71 631 605 750 2481 1525
Fragments arranged by size:
2481 1525 750 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6287 6287
Size: 6292
BlpI GC'TnA_GC
Cuts at: 2112 2741 3709 2112
Size: 629 968 4695
Fragments arranged by size:
4695 968 629
Bme1580I G_kGCm'C
Cuts at: 1790 2305 2752 2847 2856 3360 3500 3558 4353
Size: 515 447 95 9 504 140 58 795
Cuts at: 4353 5509 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 795 515 504 447 140 95
58 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6292
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2299 4023 4981
Size: 416 53 83 399 24 882 1724 958
Cuts at: 4981 442
Size: 1753
Fragments arranged by size:
1753 1724 958 882 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6292
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2379 2379
Size: 6292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2162 2304 4952 1239
Size: 923 142 2648 2579
Fragments arranged by size:
2648 2579 923 142
Bpu10I CC'TnA_GC
Cuts at: 2858 2858
Size: 6292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4417 5282 5580 4417
Size: 865 298 5129
Fragments arranged by size:
5129 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2138 2280 4955 882
Size: 617 639 142 2675 2219
Fragments arranged by size:
2675 2219 639 617 142
BsaAI yAC'GTr
Cuts at: 493 1204 2557 2990 3361 4083 493
Size: 711 1353 433 371 722 2702
Fragments arranged by size:
2702 1353 722 711 433 371
BsaBI GATnn'nnATC
Cuts at: 2084 3513 6286 2084
Size: 1429 2773 2090
Fragments arranged by size:
2773 2090 1429
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2193 2810 2836 3096
Size: 53 83 186 936 660 617 26 260
Cuts at: 3096 4099 4481 5116 275
Size: 1003 382 635 1451
Fragments arranged by size:
1451 1003 936 660 635 617 382 260
186 83 53 26
BsaWI w'CCGG_w
Cuts at: 2535 3160 4725 5123 5397 5544 5955 2535
Size: 625 1565 398 274 147 411 2872
Fragments arranged by size:
2872 1565 625 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6067
Fragments arranged by size:
6067 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2527 2579 2584 1186
Size: 871 470 52 5 4894
Fragments arranged by size:
4894 871 470 52 5
BseYI C'CCAG_C
Cuts at: 2123 2257 2824 3059 5495 5991 2123
Size: 134 567 235 2436 496 2424
Fragments arranged by size:
2436 2424 567 496 235 134
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2186 2484 2852 3367 3418 3500 3549
Size: 573 450 298 368 515 51 82 49
Cuts at: 3549 1163
Size: 3906
Fragments arranged by size:
3906 573 515 450 368 298 82 51
49
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3742 4503 5531 1540
Size: 275 186 1741 761 1028 2301
Fragments arranged by size:
2301 1741 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3360 3558 4353 4438 5509 3360
Size: 198 795 85 1071 4143
Fragments arranged by size:
4143 1071 795 198 85
BsmI GAATG_Cn'
Cuts at: 2043 3803 3896 2043
Size: 1760 93 4439
Fragments arranged by size:
4439 1760 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2644 2644
Size: 6292
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2208 2305 2752 2847 2856 3360
Size: 399 277 141 97 447 95 9 504
Cuts at: 3360 3500 3558 3611 4353 4438 5509 1391
Size: 140 58 53 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 504 447 399 277 141
140 97 95 85 58 53 9
BspEI T'CCGG_A
Cuts at: 5955 5955
Size: 6292
BspHI T'CATG_A
Cuts at: 2953 3333 4181 5911 2953
Size: 380 848 1730 3334
Fragments arranged by size:
3334 1730 848 380
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2298 3647 844
Size: 1454 1349 3489
Fragments arranged by size:
3489 1454 1349
BsrBI CCG'CTC
Cuts at: 1394 1823 4179 1394
Size: 429 2356 3507
Fragments arranged by size:
3507 2356 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2030 4787 4961 65
Size: 1183 782 2757 174 1396
Fragments arranged by size:
2757 1396 1183 782 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2161 2261 2619 4936 5123 1700
Size: 110 351 100 358 2317 187 2869
Fragments arranged by size:
2869 2317 358 351 187 110 100
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6292
BssHII G'CGCG_C
Cuts at: 1925 2778 2780 1925
Size: 853 2 5437
Fragments arranged by size:
5437 853 2
BssSI C'ACGA_G
Cuts at: 3410 4352 5364 3410
Size: 942 1012 4338
Fragments arranged by size:
4338 1012 942
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2710 794
Size: 1916 4376
BstBI TT'CG_AA
Cuts at: 5096 5096
Size: 6292
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6292
BstYI r'GATC_y
Cuts at: 1972 3622 3989 4374 4391 5843 5929 5941 6287
Size: 1650 367 385 17 1452 86 12 346
Cuts at: 6287 1972
Size: 1977
Fragments arranged by size:
1977 1650 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2477 2719 2875 513
Size: 552 1412 242 156 3930
Fragments arranged by size:
3930 1412 552 242 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2690 3500 487
Size: 683 56 1464 810 3279
Fragments arranged by size:
3279 1464 810 683 56
BtsI GCAGTG_nn'
Cuts at: 3556 3808 4602 4622 3556
Size: 252 794 20 5226
Fragments arranged by size:
5226 794 252 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6257
DraI TTT'AAA
Cuts at: 3596 3596
Size: 6292
DraIII CAC_nnn'GTG
Cuts at: 2710 2710
Size: 6292
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5299 809
Size: 507 3983 1802
Fragments arranged by size:
3983 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3739 4628 6257
Size: 54 1425 50 275 18 1909 889 1629
Cuts at: 6257 8
Size: 43
Fragments arranged by size:
1909 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3739 1537
Size: 275 1927 4090
Fragments arranged by size:
4090 1927 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2522 2647 2932 4222 1353
Size: 1169 125 285 1290 3423
Fragments arranged by size:
3423 1290 1169 285 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4879 5253 5399 387
Size: 1064 138 3290 374 146 1280
Fragments arranged by size:
3290 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2304 4355 4952 5739 6007 1239
Size: 393 530 142 2051 597 787 268 1524
Fragments arranged by size:
2051 1524 787 597 530 393 268 142
EcoNI CCTnn'n_nnAGG
Cuts at: 2669 2669
Size: 6292
EcoO109I rG'GnC_Cy
Cuts at: 2221 2279 2607 3115 2221
Size: 58 328 508 5398
Fragments arranged by size:
5398 508 328 58
EcoRI G'AATT_C
Cuts at: 3601 3979 3601
Size: 378 5914
FspI TGC'GCA
Cuts at: 2885 4798 2885
Size: 1913 4379
HaeII r_GCGC'y
Cuts at: 1678 2196 2243 2813 2839 3487 5439 1678
Size: 518 47 570 26 648 1952 2531
Fragments arranged by size:
2531 1952 648 570 518 47 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4933 4965 5007 5039 1373
Size: 32 135 32 3361 32 42 32 2626
Fragments arranged by size:
3361 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3636 3882 669
Size: 988 281 1698 246 3079
Fragments arranged by size:
3079 1698 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6292
HpaI GTT'AAC
Cuts at: 3882 3882
Size: 6292
KasI G'GCGC_C
Cuts at: 2192 2809 2835 2192
Size: 617 26 5649
Fragments arranged by size:
5649 617 26
KpnI G_GTAC'C
Cuts at: 3617 3617
Size: 6292
MfeI C'AATT_G
Cuts at: 3891 3891
Size: 6292
MluI A'CGCG_T
Cuts at: 6168 6168
Size: 6292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2541 5406 5590 1305
Size: 1236 2865 184 2007
Fragments arranged by size:
2865 2007 1236 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4470
Fragments arranged by size:
4470 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3104 3335 3363 4250 4609 4768 518
Size: 648 1938 231 28 887 359 159 2042
Fragments arranged by size:
2042 1938 887 648 359 231 159 28
MspA1I CmG'CkG
Cuts at: 1606 2111 2179 2721 2755 2767 2866 2877 2889
Size: 505 68 542 34 12 99 11 12
Cuts at: 2889 3708 4385 5533 5778 1606
Size: 819 677 1148 245 2120
Fragments arranged by size:
2120 1148 819 677 542 505 245 99
68 34 12 12 11
NarI GG'CG_CC
Cuts at: 2193 2810 2836 2193
Size: 617 26 5649
Fragments arranged by size:
5649 617 26
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5740
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6292
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6292
NotI GC'GGCC_GC
Cuts at: 3739 3739
Size: 6292
NspI r_CATG'y
Cuts at: 1132 1229 3650 5195 1132
Size: 97 2421 1545 2229
Fragments arranged by size:
2421 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6292
PciI A'CATG_T
Cuts at: 5191 5191
Size: 6292
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2560 3311 1108
Size: 1452 751 4089
Fragments arranged by size:
4089 1452 751
PfoI T'CCnGG_A
Cuts at: 2139 3267 2139
Size: 1128 5164
PmeI GTTT'AAAC
Cuts at: 3596 3596
Size: 6292
PmlI CAC'GTG
Cuts at: 2557 2990 3361 2557
Size: 433 371 5488
Fragments arranged by size:
5488 433 371
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4361 4393 5874 5906 4361
Size: 32 1481 32 4747
Fragments arranged by size:
4747 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3115 3115
Size: 6292
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6292
PsiI TTA'TAA
Cuts at: 3862 3862
Size: 6292
PspOMI G'GGCC_C
Cuts at: 2301 2301
Size: 6292
PstI C_TGCA'G
Cuts at: 830 2295 2670 2911 3644 830
Size: 1465 375 241 733 3478
Fragments arranged by size:
3478 1465 733 375 241
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6292
PvuII CAG'CTG
Cuts at: 2755 2767 2866 2889 2755
Size: 12 99 23 6158
Fragments arranged by size:
6158 99 23 12
SacII CC_GC'GG
Cuts at: 2722 2878 2722
Size: 156 6136
SalI G'TCGA_C
Cuts at: 1936 3634 1936
Size: 1698 4594
SanDI GG'GwC_CC
Cuts at: 3115 3115
Size: 6292
SbfI CC_TGCA'GG
Cuts at: 3644 3644
Size: 6292
ScaI AGT'ACT
Cuts at: 4540 6252 4540
Size: 1712 4580
SfcI C'TryA_G
Cuts at: 826 1045 2291 2666 2907 3640 4775 5456 5647
Size: 219 1246 375 241 733 1135 681 191
Cuts at: 5647 826
Size: 1471
Fragments arranged by size:
1471 1246 1135 733 681 375 241 219
191
SfoI GGC'GCC
Cuts at: 2194 2811 2837 2194
Size: 617 26 5649
Fragments arranged by size:
5649 617 26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6292
SmaI CCC'GGG
Cuts at: 1872 2464 3619 1872
Size: 592 1155 4545
Fragments arranged by size:
4545 1155 592
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4396 5297 5559 820
Size: 197 926 2453 901 262 1553
Fragments arranged by size:
2453 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4083 493
Size: 3590 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6292
SphI G_CATG'C
Cuts at: 1132 3650 1132
Size: 2518 3774
SspI AAT'ATT
Cuts at: 5 52 2052 4216 5
Size: 47 2000 2164 2081
Fragments arranged by size:
2164 2081 2000 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6292
StyI C'CwwG_G
Cuts at: 513 1065 2931 3731 513
Size: 552 1866 800 3074
Fragments arranged by size:
3074 1866 800 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2271 3468 4319 4336 4489 4674 5119
Size: 1255 691 1197 851 17 153 185 445
Cuts at: 5119 325
Size: 1498
Fragments arranged by size:
1498 1255 1197 851 691 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2589 3417 4538
Size: 274 80 33 51 1443 612 828 1121
Cuts at: 4538 6250 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1121 828 612 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6252 6284 6252
Size: 32 6260
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6292
XbaI T'CTAG_A
Cuts at: 3628 3628
Size: 6292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2312 2312
Size: 6292
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6292
XmaI C'CCGG_G
Cuts at: 1870 2462 3617 1870
Size: 592 1155 4545
Fragments arranged by size:
4545 1155 592
XmnI GAAnn'nnTTC
Cuts at: 4421 4421
Size: 6292
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3097 4100 276
Size: 53 83 186 936 1563 1003 2468
Fragments arranged by size:
2468 1563 1003 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII
SacI SapI SexAI SfiI SrfI SwaI