Restriction map for
pFN21AA1868
(Circular) (Six-base) MAPSORT of: pFN21AA1868.seq Check: 1484 from: 1 to: 6700
pFN21AA1868 6700 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4510 278
Size: 53 83 186 936 2974 2468
Fragments arranged by size:
2974 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4043 1937
Size: 2106 4594
Acc65I G'GTAC_C
Cuts at: 3990 4021 3990
Size: 31 6669
AclI AA'CG_TT
Cuts at: 1563 4827 5200 1563
Size: 3264 373 3063
Fragments arranged by size:
3264 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2987 3119 3290 3419 4763 6147 6415 1632
Size: 1355 132 171 129 1344 1384 268 1917
Fragments arranged by size:
1917 1384 1355 1344 268 171 132 129
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6503
AflIII A'CryG_T
Cuts at: 1203 3486 5599 6576 1203
Size: 2283 2113 977 1327
Fragments arranged by size:
2283 2113 1327 977
AgeI A'CCGG_T
Cuts at: 5531 5531
Size: 6700
AhdI GACnn_n'nnGTC
Cuts at: 3415 5429 3415
Size: 2014 4686
AleI CACnn'nnGTG
Cuts at: 1166 3242 1166
Size: 2076 4624
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6700
AlwNI CAG_nnn'CTG
Cuts at: 2290 2653 2848 3022 3142 3256 4443 6015 2290
Size: 363 195 174 120 114 1187 1572 2975
Fragments arranged by size:
2975 1572 1187 363 195 174 120 114
ApaI G_GGCC'C
Cuts at: 3319 3319
Size: 6700
ApaLI G'TGCA_C
Cuts at: 2480 4757 5913 2480
Size: 2277 1156 3267
Fragments arranged by size:
3267 2277 1156
ApoI r'AATT_y
Cuts at: 1493 2072 2294 3695 3715 4009 4235 4387 6496
Size: 579 222 1401 20 294 226 152 2109
Cuts at: 6496 1493
Size: 1697
Fragments arranged by size:
2109 1697 1401 579 294 226 222 152
20
AseI AT'TA_AT
Cuts at: 160 1032 5254 160
Size: 872 4222 1606
Fragments arranged by size:
4222 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6700
AvaI C'yCGr_G
Cuts at: 1870 1943 4025 1870
Size: 73 2082 4545
Fragments arranged by size:
4545 2082 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3548 3581 3548
Size: 33 6667
BamHI G'GATC_C
Cuts at: 4030 4397 4030
Size: 367 6333
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2911 3339 3990 4021 5476
Size: 325 217 627 1124 428 651 31 1455
Cuts at: 5476 618
Size: 1842
Fragments arranged by size:
1842 1455 1124 651 627 428 325 217
31
BanII G_rGCy'C
Cuts at: 1391 3319 3580 3951 4019 1391
Size: 1928 261 371 68 4072
Fragments arranged by size:
4072 1928 371 261 68
BbeI G_GCGC'C
Cuts at: 3343 3343
Size: 6700
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2085 3645 3908 928
Size: 971 186 1560 263 3720
Fragments arranged by size:
3720 1560 971 263 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4891 4925 1903
Size: 34 2954 34 3678
Fragments arranged by size:
3678 2954 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4592 5802 4592
Size: 1210 5490
BclI T'GATC_A
Cuts at: 1548 2784 1548
Size: 1236 5464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2909 3500 5311
Size: 107 122 71 631 605 1237 591 1811
Cuts at: 5311 136
Size: 1525
Fragments arranged by size:
1811 1525 1237 631 605 591 122 107
71
BglII A'GATC_T
Cuts at: 6695 6695
Size: 6700
BlpI GC'TnA_GC
Cuts at: 4117 4117
Size: 6700
Bme1580I G_kGCm'C
Cuts at: 1790 2484 3319 4761 5917 1790
Size: 694 835 1442 1156 2573
Fragments arranged by size:
2573 1442 1156 835 694
BmgBI CAC'GTC
Cuts at: 1318 3160 1318
Size: 1842 4858
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3989 4431 5389
Size: 416 53 83 399 24 2572 442 958
Cuts at: 5389 442
Size: 1753
Fragments arranged by size:
2572 1753 958 442 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2420 2505 2786 3072 3278 3452 5360 1239
Size: 1181 85 281 286 206 174 1908 2579
Fragments arranged by size:
2579 1908 1181 286 281 206 174 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2440 4825 5690 5988 2440
Size: 2385 865 298 3152
Fragments arranged by size:
3152 2385 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2205 2341 2396 3059 5363 882
Size: 617 706 136 55 663 2304 2219
Fragments arranged by size:
2304 2219 706 663 617 136 55
BsaAI yAC'GTr
Cuts at: 493 1204 4491 493
Size: 711 3287 2702
Fragments arranged by size:
3287 2702 711
BsaBI GATnn'nnATC
Cuts at: 6694 6694
Size: 6700
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3340 4507 4889 5524
Size: 53 83 186 936 1807 1167 382 635
Cuts at: 5524 275
Size: 1451
Fragments arranged by size:
1807 1451 1167 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2350 5133 5531 5805 5952 6363 2350
Size: 2783 398 274 147 411 2687
Fragments arranged by size:
2783 2687 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6475
Fragments arranged by size:
6475 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 2375 3139 3290 1186
Size: 1069 120 764 151 4596
Fragments arranged by size:
4596 1069 764 151 120
BseYI C'CCAG_C
Cuts at: 3831 5903 6399 3831
Size: 2072 496 4132
Fragments arranged by size:
4132 2072 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3128 3950 1163
Size: 573 1392 822 3913
Fragments arranged by size:
3913 1392 822 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4150 4911 5939 1540
Size: 275 186 2149 761 1028 2301
Fragments arranged by size:
2301 2149 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2247 2484 3516 3580 4761 4846 5917 2247
Size: 237 1032 64 1181 85 1071 3030
Fragments arranged by size:
3030 1181 1071 1032 237 85 64
BsmI GAATG_Cn'
Cuts at: 2111 4211 4304 2111
Size: 2100 93 4507
Fragments arranged by size:
4507 2100 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2247 2484 3319 3516 3580 3951 4019
Size: 399 457 237 835 197 64 371 68
Cuts at: 4019 4761 4846 5917 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 835 742 457 399 371 237
197 85 68 64
BspEI T'CCGG_A
Cuts at: 6363 6363
Size: 6700
BspHI T'CATG_A
Cuts at: 2023 3453 3885 4589 6319 2023
Size: 1430 432 704 1730 2404
Fragments arranged by size:
2404 1730 1430 704 432
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4055 844
Size: 3211 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3939 4587 1394
Size: 429 2116 648 3507
Fragments arranged by size:
3507 2116 648 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3097 3633 3676 5195 5369 65
Size: 1183 1849 536 43 1519 174 1396
Fragments arranged by size:
1849 1519 1396 1183 536 174 43
BsrFI r'CCGG_y
Cuts at: 1700 1810 5344 5531 1700
Size: 110 3534 187 2869
Fragments arranged by size:
3534 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3582 96
Size: 3486 3214
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6700
BssSI C'ACGA_G
Cuts at: 2869 3151 3509 4760 5772 2869
Size: 282 358 1251 1012 3797
Fragments arranged by size:
3797 1251 1012 358 282
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3022 794
Size: 2228 4472
BstBI TT'CG_AA
Cuts at: 5504 5504
Size: 6700
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6700
BstXI CCAn_nnnn'nTGG
Cuts at: 2476 2476
Size: 6700
BstYI r'GATC_y
Cuts at: 1972 2147 2185 2703 3529 4030 4397 4782 4799
Size: 175 38 518 826 501 367 385 17
Cuts at: 4799 6251 6337 6349 6695 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 826 518 501 385 367 346
175 86 38 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3867 513
Size: 552 939 1863 3346
Fragments arranged by size:
3346 1863 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3371 487
Size: 683 56 2145 3816
Fragments arranged by size:
3816 2145 683 56
BtsI GCAGTG_nn'
Cuts at: 3938 4216 5010 5030 3938
Size: 278 794 20 5608
Fragments arranged by size:
5608 794 278 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6700
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6665
DraI TTT'AAA
Cuts at: 2494 4004 2494
Size: 1510 5190
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5707 809
Size: 507 4391 1802
Fragments arranged by size:
4391 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3177 3538 3976
Size: 54 1425 50 275 18 1347 361 438
Cuts at: 3976 4147 5036 6665 8
Size: 171 889 1629 43
Fragments arranged by size:
1629 1425 1347 889 438 361 275 171
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4147 1537
Size: 275 2335 4090
Fragments arranged by size:
4090 2335 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2507 3717 3771 4630 1353
Size: 1154 1210 54 859 3423
Fragments arranged by size:
3423 1210 1154 859 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2160 2807 3674 3925 5287 5661
Size: 1064 138 571 647 867 251 1362 374
Cuts at: 5661 5807 387
Size: 146 1280
Fragments arranged by size:
1362 1280 1064 867 647 571 374 251
146 138
EcoICRI GAG'CTC
Cuts at: 3578 3578
Size: 6700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2420 2505 2786 2987 3072 3119 3278
Size: 393 788 85 281 201 85 47 159
Cuts at: 3278 3290 3419 3452 4763 5360 6147 6415 1239
Size: 12 129 33 1311 597 787 268 1524
Fragments arranged by size:
1524 1311 788 787 597 393 281 268
201 159 129 85 85 47 33 12
EcoO109I rG'GnC_Cy
Cuts at: 3315 3316 3403 3315
Size: 1 87 6612
Fragments arranged by size:
6612 87 1
EcoRI G'AATT_C
Cuts at: 2294 3695 4009 4387 2294
Size: 1401 314 378 4607
Fragments arranged by size:
4607 1401 378 314
EcoRV GAT'ATC
Cuts at: 3289 3289
Size: 6700
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 6700
FspI TGC'GCA
Cuts at: 5206 5206
Size: 6700
HaeII r_GCGC'y
Cuts at: 1678 3034 3343 5847 1678
Size: 1356 309 2504 2531
Fragments arranged by size:
2531 2504 1356 309
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2150 2182 2258 2290 3401
Size: 32 135 32 578 32 76 32 1111
Cuts at: 3401 3433 5341 5373 5415 5447 1373
Size: 32 1908 32 42 32 2626
Fragments arranged by size:
2626 1908 1111 578 135 76 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4044 4290 669
Size: 988 281 2106 246 3079
Fragments arranged by size:
3079 2106 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2585 3857 748
Size: 1837 1272 3591
Fragments arranged by size:
3591 1837 1272
HpaI GTT'AAC
Cuts at: 4290 4290
Size: 6700
KasI G'GCGC_C
Cuts at: 3339 3339
Size: 6700
KpnI G_GTAC'C
Cuts at: 3994 4025 3994
Size: 31 6669
MfeI C'AATT_G
Cuts at: 4299 4299
Size: 6700
MluI A'CGCG_T
Cuts at: 6576 6576
Size: 6700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2520 5814 5998 1305
Size: 1215 3294 184 2007
Fragments arranged by size:
3294 2007 1215 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3179 3540 10
Size: 54 1425 343 1347 361 3170
Fragments arranged by size:
3170 1425 1347 361 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3242 4658 5017 5176 518
Size: 648 2076 1416 359 159 2042
Fragments arranged by size:
2076 2042 1416 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2653 2775 3346 4116 4793 5941 6186 1606
Size: 1047 122 571 770 677 1148 245 2120
Fragments arranged by size:
2120 1148 1047 770 677 571 245 122
NarI GG'CG_CC
Cuts at: 3340 3340
Size: 6700
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5209
Fragments arranged by size:
5209 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6700
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6700
NotI GC'GGCC_GC
Cuts at: 4147 4147
Size: 6700
NspI r_CATG'y
Cuts at: 1132 1229 3490 4058 5603 1132
Size: 97 2261 568 1545 2229
Fragments arranged by size:
2261 2229 1545 568 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6700
PciI A'CATG_T
Cuts at: 3486 5599 3486
Size: 2113 4587
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2290 2653 1108
Size: 1182 363 5155
Fragments arranged by size:
5155 1182 363
PfoI T'CCnGG_A
Cuts at: 2505 2513 3049 2505
Size: 8 536 6156
Fragments arranged by size:
6156 536 8
PmeI GTTT'AAAC
Cuts at: 4004 4004
Size: 6700
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4769 4801 6282 6314 4769
Size: 32 1481 32 5155
Fragments arranged by size:
5155 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3403 3403
Size: 6700
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6700
PsiI TTA'TAA
Cuts at: 4270 4270
Size: 6700
PspOMI G'GGCC_C
Cuts at: 3315 3315
Size: 6700
PstI C_TGCA'G
Cuts at: 830 3252 3324 3351 4052 830
Size: 2422 72 27 701 3478
Fragments arranged by size:
3478 2422 701 72 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6700
PvuII CAG'CTG
Cuts at: 2653 2653
Size: 6700
SacI G_AGCT'C
Cuts at: 3580 3580
Size: 6700
SalI G'TCGA_C
Cuts at: 1936 4042 1936
Size: 2106 4594
SapI GCTCTTCn'nnn_
Cuts at: 3717 3717
Size: 6700
SbfI CC_TGCA'GG
Cuts at: 4052 4052
Size: 6700
ScaI AGT'ACT
Cuts at: 2336 3437 4948 6660 2336
Size: 1101 1511 1712 2376
Fragments arranged by size:
2376 1712 1511 1101
SfcI C'TryA_G
Cuts at: 826 1045 3248 3320 3347 4048 5183 5864 6055
Size: 219 2203 72 27 701 1135 681 191
Cuts at: 6055 826
Size: 1471
Fragments arranged by size:
2203 1471 1135 701 681 219 191 72
27
SfoI GGC'GCC
Cuts at: 3341 3341
Size: 6700
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6700
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6700
SmaI CCC'GGG
Cuts at: 1872 4027 1872
Size: 2155 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2419 4804 5705 5967 820
Size: 197 926 476 2385 901 262 1553
Fragments arranged by size:
2385 1553 926 901 476 262 197
SnaBI TAC'GTA
Cuts at: 493 4491 493
Size: 3998 2702
SpeI A'CTAG_T
Cuts at: 152 2044 152
Size: 1892 4808
SphI G_CATG'C
Cuts at: 1132 4058 1132
Size: 2926 3774
SspI AAT'ATT
Cuts at: 5 52 4624 5
Size: 47 4572 2081
Fragments arranged by size:
4572 2081 47
StuI AGG'CCT
Cuts at: 1517 2875 3507 1517
Size: 1358 632 4710
Fragments arranged by size:
4710 1358 632
StyI C'CwwG_G
Cuts at: 513 1065 2004 2907 2949 3495 4139 513
Size: 552 939 903 42 546 644 3074
Fragments arranged by size:
3074 939 903 644 552 546 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4727 4744 4897 5082 5527 325
Size: 1255 3147 17 153 185 445 1498
Fragments arranged by size:
3147 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2334 3171 3435
Size: 274 80 33 51 1443 357 837 264
Cuts at: 3435 3582 4946 6658 96
Size: 147 1364 1712 138
Fragments arranged by size:
1712 1443 1364 837 357 274 264 147
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3031 3063 6660 6692 3031
Size: 32 3597 32 3039
Fragments arranged by size:
3597 3039 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6700
XbaI T'CTAG_A
Cuts at: 2758 4036 2758
Size: 1278 5422
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2726 3157 3874 2726
Size: 431 717 5552
Fragments arranged by size:
5552 717 431
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6700
XmaI C'CCGG_G
Cuts at: 1870 4025 1870
Size: 2155 4545
XmnI GAAnn'nnTTC
Cuts at: 2222 2547 3005 4829 2222
Size: 325 458 1824 4093
Fragments arranged by size:
4093 1824 458 325
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4508 276
Size: 53 83 186 936 2974 2468
Fragments arranged by size:
2974 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI