Restriction map for
pFN21AA1866
(Circular) (Six-base) MAPSORT of: pFN21AA1866.seq Check: 4166 from: 1 to: 10024
pFN21AA1866 10024 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4984 5012 4984
Size: 28 9996
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6350 7834 278
Size: 53 83 186 936 4814 1484 2468
Fragments arranged by size:
4814 2468 1484 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2050 2580 3269 3370 4211 7367 1937
Size: 113 530 689 101 841 3156 4594
Fragments arranged by size:
4594 3156 841 689 530 113 101
Acc65I G'GTAC_C
Cuts at: 5772 7345 5772
Size: 1573 8451
AclI AA'CG_TT
Cuts at: 1563 8151 8524 1563
Size: 6588 373 3063
Fragments arranged by size:
6588 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3248 6276 6456 6605 8087 9471 9739 1632
Size: 1616 3028 180 149 1482 1384 268 1917
Fragments arranged by size:
3028 1917 1616 1482 1384 268 180 149
AfeI AGC'GCT
Cuts at: 3429 5455 3429
Size: 2026 7998
AflII C'TTAA_G
Cuts at: 820 1017 4384 820
Size: 197 3367 6460
Fragments arranged by size:
6460 3367 197
AflIII A'CryG_T
Cuts at: 1203 3528 4584 8923 9900 1203
Size: 2325 1056 4339 977 1327
Fragments arranged by size:
4339 2325 1327 1056 977
AgeI A'CCGG_T
Cuts at: 8855 8855
Size: 10024
AhdI GACnn_n'nnGTC
Cuts at: 2365 5035 5245 8753 2365
Size: 2670 210 3508 3636
Fragments arranged by size:
3636 3508 2670 210
AleI CACnn'nnGTG
Cuts at: 1166 6632 1166
Size: 5466 4558
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10024
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4433 4465 6740 6772 4433
Size: 32 2275 32 7685
Fragments arranged by size:
7685 2275 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2834 2918 2936 3011 4081 7767 9339 2834
Size: 84 18 75 1070 3686 1572 3519
Fragments arranged by size:
3686 3519 1572 1070 84 75 18
ApaI G_GGCC'C
Cuts at: 3418 4090 4663 4939 3418
Size: 672 573 276 8503
Fragments arranged by size:
8503 672 573 276
ApaLI G'TGCA_C
Cuts at: 3719 8081 9237 3719
Size: 4362 1156 4506
Fragments arranged by size:
4506 4362 1156
ApoI r'AATT_y
Cuts at: 1493 2843 6338 6443 7333 7559 7711 9820 1493
Size: 1350 3495 105 890 226 152 2109 1697
Fragments arranged by size:
3495 2109 1697 1350 890 226 152 105
AseI AT'TA_AT
Cuts at: 160 1032 8578 160
Size: 872 7546 1606
Fragments arranged by size:
7546 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10024
AvaI C'yCGr_G
Cuts at: 1870 1943 3303 4224 4368 4708 5103 6055 7349
Size: 73 1360 921 144 340 395 952 1294
Cuts at: 7349 1870
Size: 4545
Fragments arranged by size:
4545 1360 1294 952 921 395 340 144
73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6279 6312 7282 7315 6279
Size: 33 970 33 8988
Fragments arranged by size:
8988 970 33 33
BamHI G'GATC_C
Cuts at: 2692 6237 6942 7354 7721 2692
Size: 3545 705 412 367 4995
Fragments arranged by size:
4995 3545 705 412 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2383 3214 3493 3542 3641
Size: 325 217 627 596 831 279 49 99
Cuts at: 3641 5480 5772 6416 7345 8800 618
Size: 1839 292 644 929 1455 1842
Fragments arranged by size:
1842 1839 1455 929 831 644 627 596
325 292 279 217 99 49
BanII G_rGCy'C
Cuts at: 1391 3112 3418 3626 3872 4090 4231 4663 4838
Size: 1721 306 208 246 218 141 432 175
Cuts at: 4838 4939 5471 6230 7343 1391
Size: 101 532 759 1113 4072
Fragments arranged by size:
4072 1721 1113 759 532 432 306 246
218 208 175 141 101
BbeI G_GCGC'C
Cuts at: 3218 3497 3645 6420 3218
Size: 279 148 2775 6822
Fragments arranged by size:
6822 2775 279 148
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2890 4050 5241 5244 6741 7134 928
Size: 971 991 1160 1191 3 1497 393 3818
Fragments arranged by size:
3818 1497 1191 1160 991 971 393 3
BbvCI CC'TCA_GC
Cuts at: 3796 3796
Size: 10024
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4897 4931 6019 6053 8215 8249 1903
Size: 34 2960 34 1088 34 2162 34 3678
Fragments arranged by size:
3678 2960 2162 1088 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4292 7916 9126 4292
Size: 3624 1210 5190
Fragments arranged by size:
5190 3624 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10024
BfrBI ATG'CAT
Cuts at: 5569 5569
Size: 10024
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3035 3245 3746
Size: 107 122 71 631 605 1363 210 501
Cuts at: 3746 4502 4964 5082 5266 8635 136
Size: 756 462 118 184 3369 1525
Fragments arranged by size:
3369 1525 1363 756 631 605 501 462
210 184 122 118 107 71
BglII A'GATC_T
Cuts at: 5839 10019 5839
Size: 4180 5844
BlpI GC'TnA_GC
Cuts at: 4271 7441 4271
Size: 3170 6854
Bme1580I G_kGCm'C
Cuts at: 1790 2322 3418 3545 3723 4090 4221 4663 4939
Size: 532 1096 127 178 367 131 442 276
Cuts at: 4939 5483 8085 9241 1790
Size: 544 2602 1156 2573
Fragments arranged by size:
2602 2573 1156 1096 544 532 442 367
276 178 131 127
BmgBI CAC'GTC
Cuts at: 1318 4695 4791 5428 1318
Size: 3377 96 637 5914
Fragments arranged by size:
5914 3377 637 96
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3291 4623 5086
Size: 416 53 83 399 24 1874 1332 463
Cuts at: 5086 6682 7755 8713 442
Size: 1596 1073 958 1753
Fragments arranged by size:
1874 1753 1596 1332 1073 958 463 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2516 1056
Size: 1460 8564
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2146 3109 4865 5388 2146
Size: 963 1756 523 6782
Fragments arranged by size:
6782 1756 963 523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2259 2418 3096 4180 4336 4822 4847 5385
Size: 1020 159 678 1084 156 486 25 538
Cuts at: 5385 8684 1239
Size: 3299 2579
Fragments arranged by size:
3299 2579 1084 1020 678 538 486 159
156 25
Bpu10I CC'TnA_GC
Cuts at: 3084 3796 3866 4266 4276 4506 3084
Size: 712 70 400 10 230 8602
Fragments arranged by size:
8602 712 400 230 70 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3793 5697 6747 8149 9014 9312 3793
Size: 1904 1050 1402 865 298 4505
Fragments arranged by size:
4505 1904 1402 1050 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2542 3994 4155 5188 6880 7309 8687
Size: 617 1043 1452 161 1033 1692 429 1378
Cuts at: 8687 882
Size: 2219
Fragments arranged by size:
2219 1692 1452 1378 1043 1033 617 429
161
BsaAI yAC'GTr
Cuts at: 493 1204 2022 4339 6484 7011 7815 493
Size: 711 818 2317 2145 527 804 2702
Fragments arranged by size:
2702 2317 2145 818 804 711 527
BsaBI GATnn'nnATC
Cuts at: 4467 10018 4467
Size: 5551 4473
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3215 3494 3642 3906
Size: 53 83 186 936 1682 279 148 264
Cuts at: 3906 5301 6032 6097 6347 6417 7831 8213 8848
Size: 1395 731 65 250 70 1414 382 635
Cuts at: 8848 275
Size: 1451
Fragments arranged by size:
1682 1451 1414 1395 936 731 635 382
279 264 250 186 148 83 70 65
53
BsaWI w'CCGG_w
Cuts at: 2346 8457 8855 9129 9276 9687 2346
Size: 6111 398 274 147 411 2683
Fragments arranged by size:
6111 2683 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2917 2947 5576 5606 6684
Size: 30 165 30 1514 30 2629 30 1078
Cuts at: 6684 6714 1178
Size: 30 4488
Fragments arranged by size:
4488 2629 1514 1078 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3266 3302 3305 3943 4310 4339 4477 4671
Size: 2080 36 3 638 367 29 138 194
Cuts at: 4671 4801 4804 5213 5216 5219 5832 1186
Size: 130 3 409 3 3 613 5378
Fragments arranged by size:
5378 2080 638 613 409 367 194 138
130 36 29 3 3 3 3
BseYI C'CCAG_C
Cuts at: 3228 3295 3651 3674 3855 3910 4374 4698 4746
Size: 67 356 23 181 55 464 324 48
Cuts at: 4746 5040 5072 5111 5172 6606 6968 7113 9227
Size: 294 32 39 61 1434 362 145 2114
Cuts at: 9227 9723 3228
Size: 496 3529
Fragments arranged by size:
3529 2114 1434 496 464 362 356 324
294 181 145 67 61 55 48 39
32 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 9451
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4657 5441 7474 8235 9263 1540
Size: 275 186 2656 784 2033 761 1028 2301
Fragments arranged by size:
2656 2301 2033 1028 784 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3112 3723 3872 4526 4838 6230 6521 8085 8170
Size: 611 149 654 312 1392 291 1564 85
Cuts at: 8170 9241 3112
Size: 1071 3895
Fragments arranged by size:
3895 1564 1392 1071 654 611 312 291
149 85
BsmI GAATG_Cn'
Cuts at: 2122 5764 7535 7628 2122
Size: 3642 1771 93 4518
Fragments arranged by size:
4518 3642 1771 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3964 4284 4719 6338 3964
Size: 320 435 1619 7650
Fragments arranged by size:
7650 1619 435 320
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2322 3112 3418 3545 3626 3723 3872
Size: 399 532 790 306 127 81 97 149
Cuts at: 3872 4090 4221 4231 4526 4663 4838 4939 5471
Size: 218 131 10 295 137 175 101 532
Cuts at: 5471 5483 6230 6521 7343 8085 8170 9241 1391
Size: 12 747 291 822 742 85 1071 2174
Fragments arranged by size:
2174 1071 822 790 747 742 532 532
399 306 295 291 218 175 149 137
131 127 101 97 85 81 12 10
BspEI T'CCGG_A
Cuts at: 9687 9687
Size: 10024
BspHI T'CATG_A
Cuts at: 7913 9643 7913
Size: 1730 8294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3976 4149 4984 5012 6606 7379 844
Size: 3132 173 835 28 1594 773 3489
Fragments arranged by size:
3489 3132 1594 835 773 173 28
BsrBI CCG'CTC
Cuts at: 1394 1823 4001 4803 7911 1394
Size: 429 2178 802 3108 3507
Fragments arranged by size:
3507 3108 2178 802 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2070 7132 8519 8693 65
Size: 1183 822 5062 1387 174 1396
Fragments arranged by size:
5062 1396 1387 1183 822 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3745 8668 8855 1700
Size: 110 1935 4923 187 2869
Fragments arranged by size:
4923 2869 1935 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10024
BssHII G'CGCG_C
Cuts at: 1925 3613 4952 4954 5509 1925
Size: 1688 1339 2 555 6440
Fragments arranged by size:
6440 1688 1339 555 2
BssSI C'ACGA_G
Cuts at: 6811 8084 9096 6811
Size: 1273 1012 7739
Fragments arranged by size:
7739 1273 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 10024
BstBI TT'CG_AA
Cuts at: 8828 8828
Size: 10024
BstEII G'GTnAC_C
Cuts at: 1184 4737 5027 1184
Size: 3553 290 6181
Fragments arranged by size:
6181 3553 290
BstYI r'GATC_y
Cuts at: 1972 2692 3259 5839 6237 6942 7354 7721 8106
Size: 720 567 2580 398 705 412 367 385
Cuts at: 8106 8123 9575 9661 9673 10019 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2580 1977 1452 720 705 567 412 398
385 367 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3356 4473 3356
Size: 1117 8907
BtgI C'CryG_G
Cuts at: 513 1065 2004 3826 4024 4068 4285 4854 5495
Size: 552 939 1822 198 44 217 569 641
Cuts at: 5495 5856 6630 6639 6985 513
Size: 361 774 9 346 3552
Fragments arranged by size:
3552 1822 939 774 641 569 552 361
346 217 198 44 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5326 487
Size: 683 56 4100 5185
Fragments arranged by size:
5185 4100 683 56
BtsI GCAGTG_nn'
Cuts at: 3414 6066 6288 6381 7540 8334 8354 3414
Size: 2652 222 93 1159 794 20 5084
Fragments arranged by size:
5084 2652 1159 794 222 93 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10024
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9989
DraI TTT'AAA
Cuts at: 5224 6826 7328 5224
Size: 1602 502 7920
Fragments arranged by size:
7920 1602 502
DraIII CAC_nnn'GTG
Cuts at: 2867 3385 2867
Size: 518 9506
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2648 9031 809
Size: 507 1332 6383 1802
Fragments arranged by size:
6383 1802 1332 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2452 2769 2954
Size: 54 1425 50 275 18 622 317 185
Cuts at: 2954 3376 3471 3659 4654 5492 5521 5913 6636
Size: 422 95 188 995 838 29 392 723
Cuts at: 6636 7471 8360 9989 8
Size: 835 889 1629 43
Fragments arranged by size:
1629 1425 995 889 838 835 723 622
422 392 317 275 188 185 95 54
50 43 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 4654 7471 1537
Size: 275 2842 2817 4090
Fragments arranged by size:
4090 2842 2817 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2610 3967 5278 5319 5805 6300 6584 7114
Size: 1257 1357 1311 41 486 495 284 530
Cuts at: 7114 7954 1353
Size: 840 3423
Fragments arranged by size:
3423 1357 1311 1257 840 530 495 486
284 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3053 4065 4076 8611 8985 9131
Size: 1064 138 1464 1012 11 4535 374 146
Cuts at: 9131 387
Size: 1280
Fragments arranged by size:
4535 1464 1280 1064 1012 374 146 138
11
EcoICRI GAG'CTC
Cuts at: 3110 3870 4836 6228 3110
Size: 760 966 1392 6906
Fragments arranged by size:
6906 1392 966 760
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2259 2418 3096 3248 4180 4336 4822
Size: 393 627 159 678 152 932 156 486
Cuts at: 4822 4847 5385 6276 6456 6605 8087 8684 9471
Size: 25 538 891 180 149 1482 597 787
Cuts at: 9471 9739 1239
Size: 268 1524
Fragments arranged by size:
1524 1482 932 891 787 678 627 597
538 486 393 268 180 159 156 152
149 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2141 4351 5318 5358 2141
Size: 2210 967 40 6807
Fragments arranged by size:
6807 2210 967 40
EcoO109I rG'GnC_Cy
Cuts at: 2026 3169 3414 3756 4178 4660 4712 4936 5037
Size: 1143 245 342 422 482 52 224 101
Cuts at: 5037 5107 5418 5850 6186 6918 7172 2026
Size: 70 311 432 336 732 254 4878
Fragments arranged by size:
4878 1143 732 482 432 422 342 336
311 254 245 224 101 70 52
EcoRI G'AATT_C
Cuts at: 7333 7711 7333
Size: 378 9646
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3478 3661 5258 3478
Size: 183 1597 8244
Fragments arranged by size:
8244 1597 183
FspI TGC'GCA
Cuts at: 8530 8530
Size: 10024
HaeII r_GCGC'y
Cuts at: 1678 3218 3431 3497 3645 5126 5457 6420 9171
Size: 1540 213 66 148 1481 331 963 2751
Cuts at: 9171 1678
Size: 2531
Fragments arranged by size:
2751 2531 1540 1481 963 331 213 148
66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2351 2383 3251 3283 3698
Size: 32 135 32 779 32 868 32 415
Cuts at: 3698 3730 5021 5053 5231 5263 6254 6286 6684
Size: 32 1291 32 178 32 991 32 398
Cuts at: 6684 6716 8665 8697 8739 8771 1373
Size: 32 1949 32 42 32 2626
Fragments arranged by size:
2626 1949 1291 991 868 779 415 398
178 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3270 4212 7368 7614 669
Size: 988 281 1332 942 3156 246 3079
Fragments arranged by size:
3156 3079 1332 988 942 281 246
HindIII A'AGCT_T
Cuts at: 748 4268 6765 748
Size: 3520 2497 4007
Fragments arranged by size:
4007 3520 2497
HpaI GTT'AAC
Cuts at: 7614 7614
Size: 10024
KasI G'GCGC_C
Cuts at: 3214 3493 3641 6416 3214
Size: 279 148 2775 6822
Fragments arranged by size:
6822 2775 279 148
KpnI G_GTAC'C
Cuts at: 5776 7349 5776
Size: 1573 8451
MfeI C'AATT_G
Cuts at: 7623 7623
Size: 10024
MluI A'CGCG_T
Cuts at: 3528 4584 9900 3528
Size: 1056 5316 3652
Fragments arranged by size:
5316 3652 1056
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5115 6125 9138 9322 1305
Size: 3810 1010 3013 184 2007
Fragments arranged by size:
3810 3013 2007 1010 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2771 2956 3473 5523 5915
Size: 54 1425 343 939 185 517 2050 392
Cuts at: 5915 10
Size: 4119
Fragments arranged by size:
4119 2050 1425 939 517 392 343 185
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2111 4629 5540 6008 6204 6629 6632
Size: 648 945 2518 911 468 196 425 3
Cuts at: 6632 7158 7982 8341 8500 518
Size: 526 824 359 159 2042
Fragments arranged by size:
2518 2042 945 911 824 648 526 468
425 359 196 159 3
MspA1I CmG'CkG
Cuts at: 1606 4970 5497 5959 7113 7440 8117 9265 9510
Size: 3364 527 462 1154 327 677 1148 245
Cuts at: 9510 1606
Size: 2120
Fragments arranged by size:
3364 2120 1154 1148 677 527 462 327
245
NaeI GCC'GGC
Cuts at: 3747 3747
Size: 10024
NarI GG'CG_CC
Cuts at: 3215 3494 3642 6417 3215
Size: 279 148 2775 6822
Fragments arranged by size:
6822 2775 279 148
NcoI C'CATG_G
Cuts at: 513 1065 2004 3826 4285 6985 513
Size: 552 939 1822 459 2700 3552
Fragments arranged by size:
3552 2700 1822 939 552 459
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10024
NgoMIV G'CCGG_C
Cuts at: 3745 3745
Size: 10024
NheI G'CTAG_C
Cuts at: 1052 2512 1052
Size: 1460 8564
NotI GC'GGCC_GC
Cuts at: 7471 7471
Size: 10024
NsiI A_TGCA'T
Cuts at: 5571 5571
Size: 10024
NspI r_CATG'y
Cuts at: 1132 1229 5054 5573 6614 7382 8927 1132
Size: 97 3825 519 1041 768 1545 2229
Fragments arranged by size:
3825 2229 1545 1041 768 519 97
PasI CC'CwG_GG
Cuts at: 1348 3488 3538 4236 4885 5286 5415 6182 1348
Size: 2140 50 698 649 401 129 767 5190
Fragments arranged by size:
5190 2140 767 698 649 401 129 50
PciI A'CATG_T
Cuts at: 8923 8923
Size: 10024
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2963 3385 3827 4996 5885 7144 1108
Size: 1855 422 442 1169 889 1259 3988
Fragments arranged by size:
3988 1855 1259 1169 889 442 422
PfoI T'CCnGG_A
Cuts at: 2281 3977 2281
Size: 1696 8328
PmeI GTTT'AAAC
Cuts at: 7328 7328
Size: 10024
PmlI CAC'GTG
Cuts at: 2022 4339 7011 2022
Size: 2317 2672 5035
Fragments arranged by size:
5035 2672 2317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7159 7191 8093 8125 9606 9638 7159
Size: 32 902 32 1481 32 7545
Fragments arranged by size:
7545 1481 902 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2026 3169 3756 4178 4712 5037 5107 5850 6186
Size: 1143 587 422 534 325 70 743 336
Cuts at: 6186 2026
Size: 5864
Fragments arranged by size:
5864 1143 743 587 534 422 336 325
70
PshAI GACnn'nnGTC
Cuts at: 1613 3986 4731 1613
Size: 2373 745 6906
Fragments arranged by size:
6906 2373 745
PsiI TTA'TAA
Cuts at: 2784 5631 7594 2784
Size: 2847 1963 5214
Fragments arranged by size:
5214 2847 1963
PspOMI G'GGCC_C
Cuts at: 3414 4086 4659 4935 3414
Size: 672 573 276 8503
Fragments arranged by size:
8503 672 573 276
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 7007 7039 7007
Size: 32 9992
PstI C_TGCA'G
Cuts at: 830 2188 2547 3409 4191 6295 7376 830
Size: 1358 359 862 782 2104 1081 3478
Fragments arranged by size:
3478 2104 1358 1081 862 782 359
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10024
PvuII CAG'CTG
Cuts at: 4970 7113 4970
Size: 2143 7881
SacI G_AGCT'C
Cuts at: 3112 3872 4838 6230 3112
Size: 760 966 1392 6906
Fragments arranged by size:
6906 1392 966 760
SacII CC_GC'GG
Cuts at: 5498 5498
Size: 10024
SalI G'TCGA_C
Cuts at: 1936 3268 4210 7366 1936
Size: 1332 942 3156 4594
Fragments arranged by size:
4594 3156 1332 942
SanDI GG'GwC_CC
Cuts at: 2026 4712 5107 5850 2026
Size: 2686 395 743 6200
Fragments arranged by size:
6200 2686 743 395
SbfI CC_TGCA'GG
Cuts at: 7376 7376
Size: 10024
ScaI AGT'ACT
Cuts at: 4484 8272 9984 4484
Size: 3788 1712 4524
Fragments arranged by size:
4524 3788 1712
SfcI C'TryA_G
Cuts at: 826 1045 2184 2543 2581 2973 3405 4187 6291
Size: 219 1139 359 38 392 432 782 2104
Cuts at: 6291 7096 7372 8507 9188 9379 826
Size: 805 276 1135 681 191 1471
Fragments arranged by size:
2104 1471 1139 1135 805 782 681 432
392 359 276 219 191 38
SfoI GGC'GCC
Cuts at: 3216 3495 3643 6418 3216
Size: 279 148 2775 6822
Fragments arranged by size:
6822 2775 279 148
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10024
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10024
SmaI CCC'GGG
Cuts at: 1872 3305 4370 4710 5105 7351 1872
Size: 1433 1065 340 395 2246 4545
Fragments arranged by size:
4545 2246 1433 1065 395 340
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3808 4384 5712 6762 8128 9029
Size: 197 926 1865 576 1328 1050 1366 901
Cuts at: 9029 9291 820
Size: 262 1553
Fragments arranged by size:
1865 1553 1366 1328 1050 926 901 576
262 197
SnaBI TAC'GTA
Cuts at: 493 7815 493
Size: 7322 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10024
SphI G_CATG'C
Cuts at: 1132 5573 6614 7382 1132
Size: 4441 1041 768 3774
Fragments arranged by size:
4441 3774 1041 768
SspI AAT'ATT
Cuts at: 5 52 3198 7948 5
Size: 47 3146 4750 2081
Fragments arranged by size:
4750 3146 2081 47
StuI AGG'CCT
Cuts at: 1517 6311 1517
Size: 4794 5230
StyI C'CwwG_G
Cuts at: 513 1065 2004 3151 3165 3636 3826 3882 4285
Size: 552 939 1147 14 471 190 56 403
Cuts at: 4285 4500 4959 5319 6189 6985 7463 513
Size: 215 459 360 870 796 478 3074
Fragments arranged by size:
3074 1147 939 870 796 552 478 471
459 403 360 215 190 56 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2047 2677 3350 3551 3869 4725 5170
Size: 1255 467 630 673 201 318 856 445
Cuts at: 5170 5498 6005 6862 8051 8068 8221 8406 8851
Size: 328 507 857 1189 17 153 185 445
Cuts at: 8851 325
Size: 1498
Fragments arranged by size:
1498 1255 1189 857 856 673 630 507
467 445 445 328 318 201 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2166 2736 4482
Size: 274 80 33 51 1443 189 570 1746
Cuts at: 4482 8270 9982 96
Size: 3788 1712 138
Fragments arranged by size:
3788 1746 1712 1443 570 274 189 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3515 3547 3883 3915 4241 4273 4328 4360 5417
Size: 32 336 32 326 32 55 32 1057
Cuts at: 5417 5449 6082 6114 9984 10016 3515
Size: 32 633 32 3870 32 3523
Fragments arranged by size:
3870 3523 1057 633 336 326 55 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10024
XbaI T'CTAG_A
Cuts at: 7360 7360
Size: 10024
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2053 5786 5839 2053
Size: 3733 53 6238
Fragments arranged by size:
6238 3733 53
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10024
XmaI C'CCGG_G
Cuts at: 1870 3303 4368 4708 5103 7349 1870
Size: 1433 1065 340 395 2246 4545
Fragments arranged by size:
4545 2246 1433 1065 395 340
XmnI GAAnn'nnTTC
Cuts at: 2608 5241 8153 2608
Size: 2633 2912 4479
Fragments arranged by size:
4479 2912 2633
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6348 7832 276
Size: 53 83 186 936 4814 1484 2468
Fragments arranged by size:
4814 2468 1484 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BsiWI BstXI BstZ17I EcoRV FseI FspAI
NruI PacI PspXI RsrII SapI SexAI SfiI SrfI
SwaI