Restriction map for
pFN21AA1853
(Circular) (Six-base) MAPSORT of: pFN21AA1853.seq Check: 9788 from: 1 to: 6538
pFN21AA1853 6538 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 4348 278
Size: 53 83 186 936 1930 882 2468
Fragments arranged by size:
2468 1930 936 882 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3881 1937
Size: 1944 4594
Acc65I G'GTAC_C
Cuts at: 3859 3859
Size: 6538
AclI AA'CG_TT
Cuts at: 1563 4665 5038 1563
Size: 3102 373 3063
Fragments arranged by size:
3102 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4601 5985 6253 1632
Size: 2969 1384 268 1917
Fragments arranged by size:
2969 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6341
AflIII A'CryG_T
Cuts at: 1203 2874 5437 6414 1203
Size: 1671 2563 977 1327
Fragments arranged by size:
2563 1671 1327 977
AgeI A'CCGG_T
Cuts at: 5369 5369
Size: 6538
AhdI GACnn_n'nnGTC
Cuts at: 5267 5267
Size: 6538
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6538
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2144 1184
Size: 960 5578
AlwNI CAG_nnn'CTG
Cuts at: 2203 4281 5853 2203
Size: 2078 1572 2888
Fragments arranged by size:
2888 2078 1572
ApaLI G'TGCA_C
Cuts at: 4595 5751 4595
Size: 1156 5382
ApoI r'AATT_y
Cuts at: 1493 3847 4073 4225 6334 1493
Size: 2354 226 152 2109 1697
Fragments arranged by size:
2354 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5092 160
Size: 872 4060 1606
Fragments arranged by size:
4060 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6538
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 2916 3505 3863 1870
Size: 73 144 829 589 358 4545
Fragments arranged by size:
4545 829 589 358 144 73
BamHI G'GATC_C
Cuts at: 3403 3868 4235 3403
Size: 465 367 5706
Fragments arranged by size:
5706 465 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2222 2264 2619 3859 5314
Size: 325 217 627 435 42 355 1240 1455
Cuts at: 5314 618
Size: 1842
Fragments arranged by size:
1842 1455 1240 627 435 355 325 217
42
BanII G_rGCy'C
Cuts at: 1391 2145 2217 2588 2939 2970 3155 3335 3740
Size: 754 72 371 351 31 185 180 405
Cuts at: 3740 3857 1391
Size: 117 4072
Fragments arranged by size:
4072 754 405 371 351 185 180 117
72 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5567
BbvCI CC'TCA_GC
Cuts at: 3593 3593
Size: 6538
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2083 2117 4729 4763 1903
Size: 34 146 34 2612 34 3678
Fragments arranged by size:
3678 2612 146 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4430 5640 4430
Size: 1210 5328
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6538
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5149 136
Size: 107 122 71 631 605 3477 1525
Fragments arranged by size:
3477 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6533 6533
Size: 6538
BlpI GC'TnA_GC
Cuts at: 2905 3808 3955 2905
Size: 903 147 5488
Fragments arranged by size:
5488 903 147
Bme1580I G_kGCm'C
Cuts at: 1790 2225 3541 4599 5755 1790
Size: 435 1316 1058 1156 2573
Fragments arranged by size:
2573 1316 1156 1058 435
BmgBI CAC'GTC
Cuts at: 1318 2877 1318
Size: 1559 4979
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2270 2707 2724
Size: 416 53 83 399 24 853 437 17
Cuts at: 2724 2790 2934 4269 5227 442
Size: 66 144 1335 958 1753
Fragments arranged by size:
1753 1335 958 853 437 416 399 144
83 66 53 24 17
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6538
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2878 3067 3442 2878
Size: 189 375 5974
Fragments arranged by size:
5974 375 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2305 2726 3190 3536 3682 3808 5198 1239
Size: 1066 421 464 346 146 126 1390 2579
Fragments arranged by size:
2579 1390 1066 464 421 346 146 126
Bpu10I CC'TnA_GC
Cuts at: 2169 3593 2169
Size: 1424 5114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2587 2971 4663 5528 5826 2587
Size: 384 1692 865 298 3299
Fragments arranged by size:
3299 1692 865 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2919 5201 882
Size: 617 1420 2282 2219
Fragments arranged by size:
2282 2219 1420 617
BsaAI yAC'GTr
Cuts at: 493 1204 4329 493
Size: 711 3125 2702
Fragments arranged by size:
3125 2702 711
BsaBI GATnn'nnATC
Cuts at: 6532 6532
Size: 6538
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3463 4345 4727 5362
Size: 53 83 186 936 1930 882 382 635
Cuts at: 5362 275
Size: 1451
Fragments arranged by size:
1930 1451 936 882 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4971 5369 5643 5790 6201 4971
Size: 398 274 147 411 5308
Fragments arranged by size:
5308 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2926 2956 3649 3679 3806
Size: 30 165 30 1523 30 693 30 127
Cuts at: 3806 3836 1178
Size: 30 3880
Fragments arranged by size:
3880 1523 693 165 127 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2461 3004 3172 3343 3568 3595 1186
Size: 1275 543 168 171 225 27 4129
Fragments arranged by size:
4129 1275 543 225 171 168 27
BseYI C'CCAG_C
Cuts at: 2164 2191 3302 3338 3389 3569 5741 6237 2164
Size: 27 1111 36 51 180 2172 496 2465
Fragments arranged by size:
2465 2172 1111 496 180 51 36 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5965
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2629 3988 4749 5777 1540
Size: 275 186 628 1359 761 1028 2301
Fragments arranged by size:
2301 1359 1028 761 628 275 186
BsiHKAI G_wGCw'C
Cuts at: 2588 2970 3155 4599 4684 5755 2588
Size: 382 185 1444 85 1071 3371
Fragments arranged by size:
3371 1444 1071 382 185 85
BsmI GAATG_Cn'
Cuts at: 4049 4142 4049
Size: 93 6445
BsmBI CGTCTCn'nnnn_
Cuts at: 3454 3676 3454
Size: 222 6316
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2217 2225 2588 2939 2970 3155
Size: 399 355 72 8 363 351 31 185
Cuts at: 3155 3335 3541 3740 3857 4599 4684 5755 1391
Size: 180 206 199 117 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 363 355 351 206
199 185 180 117 85 72 31 8
BspEI T'CCGG_A
Cuts at: 6201 6201
Size: 6538
BspHI T'CATG_A
Cuts at: 2290 4427 6157 2290
Size: 2137 1730 2671
Fragments arranged by size:
2671 2137 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2430 3893 844
Size: 1586 1463 3489
Fragments arranged by size:
3489 1586 1463
BsrBI CCG'CTC
Cuts at: 1394 1823 2827 3297 3400 3633 4425 1394
Size: 429 1004 470 103 233 792 3507
Fragments arranged by size:
3507 1004 792 470 429 233 103
BsrDI GCAATG_nn'
Cuts at: 65 1248 5033 5207 65
Size: 1183 3785 174 1396
Fragments arranged by size:
3785 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2529 2628 5182 5369 1700
Size: 110 719 99 2554 187 2869
Fragments arranged by size:
2869 2554 719 187 110 99
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6538
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6538
BssSI C'ACGA_G
Cuts at: 3700 3829 4598 5610 3700
Size: 129 769 1012 4628
Fragments arranged by size:
4628 1012 769 129
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6538
BstBI TT'CG_AA
Cuts at: 5342 5342
Size: 6538
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6538
BstXI CCAn_nnnn'nTGG
Cuts at: 2750 2750
Size: 6538
BstYI r'GATC_y
Cuts at: 1972 3403 3868 4235 4620 4637 6089 6175 6187
Size: 1431 465 367 385 17 1452 86 12
Cuts at: 6187 6533 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1431 465 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2637 2706 2637
Size: 69 6469
BtgI C'CryG_G
Cuts at: 513 1065 2004 2623 513
Size: 552 939 619 4428
Fragments arranged by size:
4428 939 619 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2373 487
Size: 683 56 1147 4652
Fragments arranged by size:
4652 1147 683 56
BtsI GCAGTG_nn'
Cuts at: 4054 4848 4868 4054
Size: 794 20 5724
Fragments arranged by size:
5724 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6538
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6503
DraI TTT'AAA
Cuts at: 3842 3842
Size: 6538
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5545 809
Size: 507 4229 1802
Fragments arranged by size:
4229 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2205 2626
Size: 54 1425 50 275 18 243 132 421
Cuts at: 2626 3636 3985 4874 6503 8
Size: 1010 349 889 1629 43
Fragments arranged by size:
1629 1425 1010 889 421 349 275 243
132 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2626 3985 1537
Size: 275 814 1359 4090
Fragments arranged by size:
4090 1359 814 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2354 2450 2488 2498 3277 4468 1353
Size: 1001 96 38 10 779 1191 3423
Fragments arranged by size:
3423 1191 1001 779 96 38 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5125 5499 5645 387
Size: 1064 138 3536 374 146 1280
Fragments arranged by size:
3536 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2586 2968 3153 2586
Size: 382 185 5971
Fragments arranged by size:
5971 382 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2305 2726 3190 3536 3682 3808 4601
Size: 393 673 421 464 346 146 126 793
Cuts at: 4601 5198 5985 6253 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 793 787 673 597 464 421 393
346 268 146 126
EcoNI CCTnn'n_nnAGG
Cuts at: 2640 2693 2640
Size: 53 6485
EcoO109I rG'GnC_Cy
Cuts at: 2370 3285 3354 2370
Size: 915 69 5554
Fragments arranged by size:
5554 915 69
EcoRI G'AATT_C
Cuts at: 3847 4225 3847
Size: 378 6160
FspI TGC'GCA
Cuts at: 5044 5044
Size: 6538
HaeII r_GCGC'y
Cuts at: 1678 3493 5685 1678
Size: 1815 2192 2531
Fragments arranged by size:
2531 2192 1815
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2165 2197 2699 2731 2864
Size: 32 135 32 593 32 502 32 133
Cuts at: 2864 2896 3649 3681 3806 3838 5179 5211 5253
Size: 32 753 32 125 32 1341 32 42
Cuts at: 5253 5285 1373
Size: 32 2626
Fragments arranged by size:
2626 1341 753 593 502 135 133 125
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2735 3882 4128 669
Size: 988 281 797 1147 246 3079
Fragments arranged by size:
3079 1147 988 797 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6538
HpaI GTT'AAC
Cuts at: 4128 4128
Size: 6538
KpnI G_GTAC'C
Cuts at: 3863 3863
Size: 6538
MfeI C'AATT_G
Cuts at: 4137 4137
Size: 6538
MluI A'CGCG_T
Cuts at: 6414 6414
Size: 6538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3021 3437 3647 5652 5836 1305
Size: 1716 416 210 2005 184 2007
Fragments arranged by size:
2007 2005 1716 416 210 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2075 2207 10
Size: 54 1425 343 243 132 4341
Fragments arranged by size:
4341 1425 343 243 132 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4496 4855 5014 518
Size: 648 3330 359 159 2042
Fragments arranged by size:
3330 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2128 2565 2625 2676 3436 3954 4631 5779
Size: 522 437 60 51 760 518 677 1148
Cuts at: 5779 6024 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 760 677 522 518 437 245
60 51
NaeI GCC'GGC
Cuts at: 2531 2531
Size: 6538
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5047
Fragments arranged by size:
5047 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6538
NgoMIV G'CCGG_C
Cuts at: 2529 2529
Size: 6538
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6538
NotI GC'GGCC_GC
Cuts at: 3985 3985
Size: 6538
NruI TCG'CGA
Cuts at: 2839 2839
Size: 6538
NspI r_CATG'y
Cuts at: 1132 1229 3896 5441 1132
Size: 97 2667 1545 2229
Fragments arranged by size:
2667 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3234 1348
Size: 1886 4652
PciI A'CATG_T
Cuts at: 5437 5437
Size: 6538
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2232 1108
Size: 1124 5414
PfoI T'CCnGG_A
Cuts at: 3653 3653
Size: 6538
PmeI GTTT'AAAC
Cuts at: 3842 3842
Size: 6538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4607 4639 6120 6152 4607
Size: 32 1481 32 4993
Fragments arranged by size:
4993 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2370 3285 3354 2370
Size: 915 69 5554
Fragments arranged by size:
5554 915 69
PshAI GACnn'nnGTC
Cuts at: 1613 2440 3466 1613
Size: 827 1026 4685
Fragments arranged by size:
4685 1026 827
PsiI TTA'TAA
Cuts at: 4108 4108
Size: 6538
PstI C_TGCA'G
Cuts at: 830 3890 830
Size: 3060 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6538
SacI G_AGCT'C
Cuts at: 2588 2970 3155 2588
Size: 382 185 5971
Fragments arranged by size:
5971 382 185
SacII CC_GC'GG
Cuts at: 2626 2626
Size: 6538
SalI G'TCGA_C
Cuts at: 1936 3880 1936
Size: 1944 4594
SbfI CC_TGCA'GG
Cuts at: 3890 3890
Size: 6538
ScaI AGT'ACT
Cuts at: 4786 6498 4786
Size: 1712 4826
SfcI C'TryA_G
Cuts at: 826 1045 2380 3382 3802 3886 5021 5702 5893
Size: 219 1335 1002 420 84 1135 681 191
Cuts at: 5893 826
Size: 1471
Fragments arranged by size:
1471 1335 1135 1002 681 420 219 191
84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6538
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6538
SmaI CCC'GGG
Cuts at: 1872 3865 1872
Size: 1993 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2602 2950 4642 5543 5805 820
Size: 197 926 659 348 1692 901 262 1553
Fragments arranged by size:
1692 1553 926 901 659 348 262 197
SnaBI TAC'GTA
Cuts at: 493 4329 493
Size: 3836 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6538
SphI G_CATG'C
Cuts at: 1132 3896 1132
Size: 2764 3774
SspI AAT'ATT
Cuts at: 5 52 4462 5
Size: 47 4410 2081
Fragments arranged by size:
4410 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6538
StyI C'CwwG_G
Cuts at: 513 1065 2004 2132 2217 3977 513
Size: 552 939 128 85 1760 3074
Fragments arranged by size:
3074 1760 939 552 128 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2252 3172 3269 3456 4565 4582 4735
Size: 1255 672 920 97 187 1109 17 153
Cuts at: 4735 4920 5365 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1109 920 672 445 187 185
153 97 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4784 6496 96
Size: 274 80 33 51 1443 2807 1712 138
Fragments arranged by size:
2807 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2068 2100 2926 2958 3520 3552 6498 6530 2068
Size: 32 826 32 562 32 2946 32 2076
Fragments arranged by size:
2946 2076 826 562 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6538
XbaI T'CTAG_A
Cuts at: 3874 3874
Size: 6538
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2215 2215
Size: 6538
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6538
XmaI C'CCGG_G
Cuts at: 1870 3863 1870
Size: 1993 4545
XmnI GAAnn'nnTTC
Cuts at: 3002 4667 3002
Size: 1665 4873
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 4346 276
Size: 53 83 186 936 1930 882 2468
Fragments arranged by size:
2468 1930 936 882 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI
KasI NarI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI
SwaI