Restriction map for
pFN21AA1849
(Circular) (Six-base) MAPSORT of: pFN21AA1849.seq Check: 6816 from: 1 to: 7924
pFN21AA1849 7924 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3868 4404 3868
Size: 536 7388
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5734 278
Size: 53 83 186 936 4198 2468
Fragments arranged by size:
4198 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3711 5267 1937
Size: 1774 1556 4594
Fragments arranged by size:
4594 1774 1556
Acc65I G'GTAC_C
Cuts at: 5245 5245
Size: 7924
AclI AA'CG_TT
Cuts at: 1563 6051 6424 1563
Size: 4488 373 3063
Fragments arranged by size:
4488 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2075 2219 4264 5987 7371 7639 1632
Size: 443 144 2045 1723 1384 268 1917
Fragments arranged by size:
2045 1917 1723 1384 443 268 144
AfeI AGC'GCT
Cuts at: 4785 4785
Size: 7924
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7727
AflIII A'CryG_T
Cuts at: 1203 6823 7800 1203
Size: 5620 977 1327
Fragments arranged by size:
5620 1327 977
AgeI A'CCGG_T
Cuts at: 6755 6755
Size: 7924
AhdI GACnn_n'nnGTC
Cuts at: 6653 6653
Size: 7924
AleI CACnn'nnGTG
Cuts at: 1166 2790 1166
Size: 1624 6300
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7924
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 7892
AlwNI CAG_nnn'CTG
Cuts at: 2220 3088 3211 3376 4030 4396 5020 5667 7239
Size: 868 123 165 654 366 624 647 1572
Cuts at: 7239 2220
Size: 2905
Fragments arranged by size:
2905 1572 868 654 647 624 366 165
123
ApaI G_GGCC'C
Cuts at: 2702 3163 3968 4126 4235 4261 2702
Size: 461 805 158 109 26 6365
Fragments arranged by size:
6365 805 461 158 109 26
ApaLI G'TGCA_C
Cuts at: 3881 5981 7137 3881
Size: 2100 1156 4668
Fragments arranged by size:
4668 2100 1156
ApoI r'AATT_y
Cuts at: 1493 5233 5459 5611 7720 1493
Size: 3740 226 152 2109 1697
Fragments arranged by size:
3740 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3107 3107
Size: 7924
AseI AT'TA_AT
Cuts at: 160 1032 6478 160
Size: 872 5446 1606
Fragments arranged by size:
5446 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7924
AvaI C'yCGr_G
Cuts at: 1870 1943 2451 2702 2960 3096 3155 3162 3815
Size: 73 508 251 258 136 59 7 653
Cuts at: 3815 3887 4271 4493 4537 4578 5000 5026 5249
Size: 72 384 222 44 41 422 26 223
Cuts at: 5249 1870
Size: 4545
Fragments arranged by size:
4545 653 508 422 384 258 251 223
222 136 73 72 59 44 41 26
7
BamHI G'GATC_C
Cuts at: 5254 5621 5254
Size: 367 7557
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2144 2258 3201 4524 4994
Size: 325 217 627 357 114 943 1323 470
Cuts at: 4994 5052 5245 6700 618
Size: 58 193 1455 1842
Fragments arranged by size:
1842 1455 1323 943 627 470 357 325
217 193 114 58
BanII G_rGCy'C
Cuts at: 1391 2169 2621 2669 2702 2712 2955 3045 3163
Size: 778 452 48 33 10 243 90 118
Cuts at: 3163 3231 3462 3546 3968 4126 4181 4235 4261
Size: 68 231 84 422 158 55 54 26
Cuts at: 4261 4621 4667 5007 5243 1391
Size: 360 46 340 236 4072
Fragments arranged by size:
4072 778 452 422 360 340 243 236
231 158 118 90 84 68 55 54
48 46 33 26 10
BbeI G_GCGC'C
Cuts at: 2148 3205 4998 5056 2148
Size: 1057 1793 58 5016
Fragments arranged by size:
5016 1793 1057 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4912 928
Size: 971 3013 3940
Fragments arranged by size:
3940 3013 971
BbvCI CC'TCA_GC
Cuts at: 2480 2480
Size: 7924
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2555 2589 6115 6149 1903
Size: 34 618 34 3526 34 3678
Fragments arranged by size:
3678 3526 618 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5084 5816 7026 5084
Size: 732 1210 5982
Fragments arranged by size:
5982 1210 732
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7924
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2361 4157 4662
Size: 107 122 71 631 605 689 1796 505
Cuts at: 4662 5103 6535 136
Size: 441 1432 1525
Fragments arranged by size:
1796 1525 1432 689 631 605 505 441
122 107 71
BglII A'GATC_T
Cuts at: 7919 7919
Size: 7924
BlpI GC'TnA_GC
Cuts at: 2099 5341 2099
Size: 3242 4682
Bme1580I G_kGCm'C
Cuts at: 1790 2702 3000 3163 3362 3764 3885 3968 4126
Size: 912 298 163 199 402 121 83 158
Cuts at: 4126 4235 4261 4493 4527 4630 5985 7141 1790
Size: 109 26 232 34 103 1355 1156 2573
Fragments arranged by size:
2573 1355 1156 912 402 298 232 199
163 158 121 109 103 83 34 26
BmgBI CAC'GTC
Cuts at: 1318 2347 2497 5182 1318
Size: 1029 150 2685 4060
Fragments arranged by size:
4060 2685 1029 150
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2663 5655 6613
Size: 416 53 83 399 24 1246 2992 958
Cuts at: 6613 442
Size: 1753
Fragments arranged by size:
2992 1753 1246 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7924
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2640 3481 3913 4635 2640
Size: 841 432 722 5929
Fragments arranged by size:
5929 841 722 432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2119 2248 2315 2824 2969 3239 3257 3437
Size: 880 129 67 509 145 270 18 180
Cuts at: 3437 3695 3776 4052 4086 4319 4550 4857 4879
Size: 258 81 276 34 233 231 307 22
Cuts at: 4879 5099 6584 1239
Size: 220 1485 2579
Fragments arranged by size:
2579 1485 880 509 307 276 270 258
233 231 220 180 145 129 81 67
34 22 18
Bpu10I CC'TnA_GC
Cuts at: 2480 2663 3974 4734 2480
Size: 183 1311 760 5670
Fragments arranged by size:
5670 1311 760 183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3365 4630 6049 6914 7212 3365
Size: 1265 1419 865 298 4077
Fragments arranged by size:
4077 1419 1265 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2648 6587 882
Size: 617 1149 3939 2219
Fragments arranged by size:
3939 2219 1149 617
BsaAI yAC'GTr
Cuts at: 493 1204 2335 4510 5715 493
Size: 711 1131 2175 1205 2702
Fragments arranged by size:
2702 2175 1205 1131 711
BsaBI GATnn'nnATC
Cuts at: 7918 7918
Size: 7924
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2145 3202 4995 5053
Size: 53 83 186 936 612 1057 1793 58
Cuts at: 5053 5731 6113 6748 275
Size: 678 382 635 1451
Fragments arranged by size:
1793 1451 1057 936 678 635 612 382
186 83 58 53
BsaWI w'CCGG_w
Cuts at: 4834 6357 6755 7029 7176 7587 4834
Size: 1523 398 274 147 411 5171
Fragments arranged by size:
5171 1523 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2108 2138 3017 3047 3704
Size: 30 165 30 705 30 879 30 657
Cuts at: 3704 3734 1178
Size: 30 5368
Fragments arranged by size:
5368 879 705 657 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2516 2613 2720 3608 3671 3692 3833 3944
Size: 1330 97 107 888 63 21 141 111
Cuts at: 3944 4877 1186
Size: 933 4233
Fragments arranged by size:
4233 1330 933 888 141 111 107 97
63 21
BseYI C'CCAG_C
Cuts at: 2175 2369 2849 3081 4125 4180 4334 4479 4570
Size: 194 480 232 1044 55 154 145 91
Cuts at: 4570 4715 4954 5062 7127 7623 2175
Size: 145 239 108 2065 496 2476
Fragments arranged by size:
2476 2065 1044 496 480 239 232 194
154 145 145 108 91 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2206 2875 2999 3622 4078 4175 4848
Size: 573 470 669 124 623 456 97 673
Cuts at: 4848 1163
Size: 4239
Fragments arranged by size:
4239 673 669 623 573 470 456 124
97
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4657 5046 5374 6135 7163 1540
Size: 275 186 2656 389 328 761 1028 2301
Fragments arranged by size:
2656 2301 1028 761 389 328 275 186
BsiHKAI G_wGCw'C
Cuts at: 2169 2621 2803 3546 3885 4074 4244 5985 6070
Size: 452 182 743 339 189 170 1741 85
Cuts at: 6070 7141 2169
Size: 1071 2952
Fragments arranged by size:
2952 1741 1071 743 452 339 189 182
170 85
BsmI GAATG_Cn'
Cuts at: 5435 5528 5435
Size: 93 7831
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2621 2669 2702 2712 2803 2955
Size: 399 379 452 48 33 10 91 152
Cuts at: 2955 3000 3045 3163 3231 3362 3462 3546 3764
Size: 45 45 118 68 131 100 84 218
Cuts at: 3764 3885 3968 4074 4126 4181 4235 4244 4261
Size: 121 83 106 52 55 54 9 17
Cuts at: 4261 4493 4527 4621 4630 4667 5007 5243 5985
Size: 232 34 94 9 37 340 236 742
Cuts at: 5985 6070 7141 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 452 399 379 340 236
232 218 152 131 121 118 106 100
94 91 85 84 83 68 55 54
52 48 45 45 37 34 33 17
10 9 9
BspEI T'CCGG_A
Cuts at: 4834 7587 4834
Size: 2753 5171
BspHI T'CATG_A
Cuts at: 3261 5813 7543 3261
Size: 2552 1730 3642
Fragments arranged by size:
3642 2552 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3036 3060 3868 4113 4404 5279 844
Size: 2192 24 808 245 291 875 3489
Fragments arranged by size:
3489 2192 875 808 291 245 24
BsrBI CCG'CTC
Cuts at: 1394 1823 2299 2911 5811 1394
Size: 429 476 612 2900 3507
Fragments arranged by size:
3507 2900 612 476 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6419 6593 65
Size: 1183 5171 174 1396
Fragments arranged by size:
5171 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2255 2324 2360 2812 2901 3135 4049
Size: 110 445 69 36 452 89 234 914
Cuts at: 4049 4194 6568 6755 1700
Size: 145 2374 187 2869
Fragments arranged by size:
2869 2374 914 452 445 234 187 145
110 89 69 36
BsrGI T'GTAC_A
Cuts at: 96 3732 96
Size: 3636 4288
BssHII G'CGCG_C
Cuts at: 1925 3107 1925
Size: 1182 6742
BssSI C'ACGA_G
Cuts at: 5984 6996 5984
Size: 1012 6912
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3376 4198 4856 794
Size: 2582 822 658 3862
Fragments arranged by size:
3862 2582 822 658
BstBI TT'CG_AA
Cuts at: 6728 6728
Size: 7924
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7924
BstXI CCAn_nnnn'nTGG
Cuts at: 2521 3469 4372 2521
Size: 948 903 6073
Fragments arranged by size:
6073 948 903
BstYI r'GATC_y
Cuts at: 1972 5199 5254 5621 6006 6023 7475 7561 7573
Size: 3227 55 367 385 17 1452 86 12
Cuts at: 7573 7919 1972
Size: 346 1977
Fragments arranged by size:
3227 1977 1452 385 367 346 86 55
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2931 3006 3019 3483 3663 4227 4657
Size: 552 1866 75 13 464 180 564 430
Cuts at: 4657 513
Size: 3780
Fragments arranged by size:
3780 1866 564 552 464 430 180 75
13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2166 2379 487
Size: 683 56 940 213 6032
Fragments arranged by size:
6032 940 683 213 56
BtsI GCAGTG_nn'
Cuts at: 2887 3710 3935 4474 5440 6234 6254 2887
Size: 823 225 539 966 794 20 4557
Fragments arranged by size:
4557 966 823 794 539 225 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7924
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2616 2651 529
Size: 35 2052 35 5802
Fragments arranged by size:
5802 2052 35 35
DraI TTT'AAA
Cuts at: 5228 5228
Size: 7924
DraIII CAC_nnn'GTG
Cuts at: 2822 4510 2822
Size: 1688 6236
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6931 809
Size: 507 5615 1802
Fragments arranged by size:
5615 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2326 2923
Size: 54 1425 50 275 18 420 76 597
Cuts at: 2923 3339 3471 3648 4110 4224 4654 4754 5043
Size: 416 132 177 462 114 430 100 289
Cuts at: 5043 5371 6260 7889 8
Size: 328 889 1629 43
Fragments arranged by size:
1629 1425 889 597 462 430 420 416
328 289 275 177 132 114 100 76
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4654 5043 5371 1537
Size: 275 2842 389 328 4090
Fragments arranged by size:
4090 2842 389 328 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2480 3923 4608 5854 1353
Size: 1127 1443 685 1246 3423
Fragments arranged by size:
3423 1443 1246 1127 685
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2888 4397 6511 6885 7031 387
Size: 1064 138 1299 1509 2114 374 146 1280
Fragments arranged by size:
2114 1509 1299 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2167 2619 3544 2167
Size: 452 925 6547
Fragments arranged by size:
6547 925 452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2119 2219 2248 2315 2824 2969
Size: 393 443 44 100 29 67 509 145
Cuts at: 2969 3239 3257 3437 3695 3776 4052 4086 4264
Size: 270 18 180 258 81 276 34 178
Cuts at: 4264 4319 4550 4857 4879 5099 5987 6584 7371
Size: 55 231 307 22 220 888 597 787
Cuts at: 7371 7639 1239
Size: 268 1524
Fragments arranged by size:
1524 888 787 597 509 443 393 307
276 270 268 258 231 220 180 178
145 100 81 67 55 44 34 29
22 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2765 4859 2765
Size: 2094 5830
EcoO109I rG'GnC_Cy
Cuts at: 2116 2698 2699 3025 3067 3159 3964 3965 4122
Size: 582 1 326 42 92 805 1 157
Cuts at: 4122 4232 4257 4473 4534 4561 4633 4744 4943
Size: 110 25 216 61 27 72 111 199
Cuts at: 4943 2116
Size: 5097
Fragments arranged by size:
5097 805 582 326 216 199 157 111
110 92 72 61 42 27 25 1
1
EcoRI G'AATT_C
Cuts at: 5233 5611 5233
Size: 378 7546
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 7924
FseI GG_CCGG'CC
Cuts at: 2328 2328
Size: 7924
FspI TGC'GCA
Cuts at: 6430 6430
Size: 7924
HaeII r_GCGC'y
Cuts at: 1678 2148 2271 2294 3205 3784 4050 4787 4998
Size: 470 123 23 911 579 266 737 211
Cuts at: 4998 5056 7071 1678
Size: 58 2015 2531
Fragments arranged by size:
2531 2015 911 737 579 470 266 211
123 58 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2108 2140 3017 3049 3779
Size: 32 135 32 536 32 877 32 730
Cuts at: 3779 3811 6565 6597 6639 6671 1373
Size: 32 2754 32 42 32 2626
Fragments arranged by size:
2754 2626 877 730 536 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2980 5268 5514 669
Size: 988 281 1042 2288 246 3079
Fragments arranged by size:
3079 2288 1042 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7924
HpaI GTT'AAC
Cuts at: 5514 5514
Size: 7924
KasI G'GCGC_C
Cuts at: 2144 3201 4994 5052 2144
Size: 1057 1793 58 5016
Fragments arranged by size:
5016 1793 1057 58
KpnI G_GTAC'C
Cuts at: 5249 5249
Size: 7924
MfeI C'AATT_G
Cuts at: 5523 5523
Size: 7924
MluI A'CGCG_T
Cuts at: 7800 7800
Size: 7924
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2472 5202 7038 7222 1305
Size: 1167 2730 1836 184 2007
Fragments arranged by size:
2730 2007 1836 1167 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2252 3473 4112 4756 10
Size: 54 1425 343 420 1221 639 644 3178
Fragments arranged by size:
3178 1425 1221 644 639 420 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2790 3668 4136 5882 6241 6400 518
Size: 648 1624 878 468 1746 359 159 2042
Fragments arranged by size:
2042 1746 1624 878 648 468 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2179 2377 2569 2687 2831 2922 2933
Size: 465 108 198 192 118 144 91 11
Cuts at: 2933 3211 3477 3601 3874 4189 4216 4815 5042
Size: 278 266 124 273 315 27 599 227
Cuts at: 5042 5340 6017 7165 7410 1606
Size: 298 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 599 465 315 298 278
273 266 245 227 198 192 144 124
118 108 91 27 11
NaeI GCC'GGC
Cuts at: 2326 2362 2903 3137 4051 2326
Size: 36 541 234 914 6199
Fragments arranged by size:
6199 914 541 234 36
NarI GG'CG_CC
Cuts at: 2145 3202 4995 5053 2145
Size: 1057 1793 58 5016
Fragments arranged by size:
5016 1793 1057 58
NcoI C'CATG_G
Cuts at: 513 1065 3483 3663 4227 513
Size: 552 2418 180 564 4210
Fragments arranged by size:
4210 2418 564 552 180
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7924
NgoMIV G'CCGG_C
Cuts at: 2324 2360 2901 3135 4049 2324
Size: 36 541 234 914 6199
Fragments arranged by size:
6199 914 541 234 36
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7924
NotI GC'GGCC_GC
Cuts at: 5371 5371
Size: 7924
NruI TCG'CGA
Cuts at: 5171 5171
Size: 7924
NspI r_CATG'y
Cuts at: 1132 1229 2086 3583 5282 6827 1132
Size: 97 857 1497 1699 1545 2229
Fragments arranged by size:
2229 1699 1545 1497 857 97
PasI CC'CwG_GG
Cuts at: 1348 2602 2695 3037 4119 4557 4623 1348
Size: 1254 93 342 1082 438 66 4649
Fragments arranged by size:
4649 1254 1082 438 342 93 66
PciI A'CATG_T
Cuts at: 6823 6823
Size: 7924
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7924
PfoI T'CCnGG_A
Cuts at: 2090 2433 2465 2913 2946 3216 3453 3753 3929
Size: 343 32 448 33 270 237 300 176
Cuts at: 3929 4029 5076 2090
Size: 100 1047 4938
Fragments arranged by size:
4938 1047 448 343 300 270 237 176
100 33 32
PmeI GTTT'AAAC
Cuts at: 5228 5228
Size: 7924
PmlI CAC'GTG
Cuts at: 4510 4510
Size: 7924
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5993 6025 7506 7538 5993
Size: 32 1481 32 6379
Fragments arranged by size:
6379 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2116 3025 3067 4561 4744 2116
Size: 909 42 1494 183 5296
Fragments arranged by size:
5296 1494 909 183 42
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7924
PsiI TTA'TAA
Cuts at: 5494 5494
Size: 7924
PspOMI G'GGCC_C
Cuts at: 2698 3159 3964 4122 4231 4257 2698
Size: 461 805 158 109 26 6365
Fragments arranged by size:
6365 805 461 158 109 26
PstI C_TGCA'G
Cuts at: 830 2568 2769 3255 3879 4023 4852 5276 830
Size: 1738 201 486 624 144 829 424 3478
Fragments arranged by size:
3478 1738 829 624 486 424 201 144
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7924
PvuII CAG'CTG
Cuts at: 2071 2179 3211 3477 3601 3874 4189 4216 2071
Size: 108 1032 266 124 273 315 27 5779
Fragments arranged by size:
5779 1032 315 273 266 124 108 27
SacI G_AGCT'C
Cuts at: 2169 2621 3546 2169
Size: 452 925 6547
Fragments arranged by size:
6547 925 452
SacII CC_GC'GG
Cuts at: 2934 2934
Size: 7924
SalI G'TCGA_C
Cuts at: 1936 5266 1936
Size: 3330 4594
SanDI GG'GwC_CC
Cuts at: 4561 4744 4561
Size: 183 7741
SbfI CC_TGCA'GG
Cuts at: 5276 5276
Size: 7924
ScaI AGT'ACT
Cuts at: 6172 7884 6172
Size: 1712 6212
SfcI C'TryA_G
Cuts at: 826 1045 2564 2765 2773 3251 3875 4019 4848
Size: 219 1519 201 8 478 624 144 829
Cuts at: 4848 5272 6407 7088 7279 826
Size: 424 1135 681 191 1471
Fragments arranged by size:
1519 1471 1135 829 681 624 478 424
219 201 191 144 8
SfoI GGC'GCC
Cuts at: 2146 3203 4996 5054 2146
Size: 1057 1793 58 5016
Fragments arranged by size:
5016 1793 1057 58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7924
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7924
SmaI CCC'GGG
Cuts at: 1872 2962 3098 3164 4580 5002 5028 5251 1872
Size: 1090 136 66 1416 422 26 223 4545
Fragments arranged by size:
4545 1416 1090 422 223 136 66 26
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3380 4609 6028 6929 7191 820
Size: 197 926 1437 1229 1419 901 262 1553
Fragments arranged by size:
1553 1437 1419 1229 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5715 493
Size: 5222 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7924
SphI G_CATG'C
Cuts at: 1132 2086 5282 1132
Size: 954 3196 3774
Fragments arranged by size:
3774 3196 954
SrfI GCCC'GGGC
Cuts at: 3098 3098
Size: 7924
SspI AAT'ATT
Cuts at: 5 52 5848 5
Size: 47 5796 2081
Fragments arranged by size:
5796 2081 47
StuI AGG'CCT
Cuts at: 1517 4007 4277 4462 1517
Size: 2490 270 185 4979
Fragments arranged by size:
4979 2490 270 185
StyI C'CwwG_G
Cuts at: 513 1065 2514 3102 3483 3663 4227 5107 5363
Size: 552 1449 588 381 180 564 880 256
Cuts at: 5363 513
Size: 3074
Fragments arranged by size:
3074 1449 880 588 564 552 381 256
180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2189 3359 4132 4336 5951 5968 6121
Size: 1255 609 1170 773 204 1615 17 153
Cuts at: 6121 6306 6751 325
Size: 185 445 1498
Fragments arranged by size:
1615 1498 1255 1170 773 609 445 204
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3549 3732 6170
Size: 274 80 33 51 1443 1572 183 2438
Cuts at: 6170 7882 96
Size: 1712 138
Fragments arranged by size:
2438 1712 1572 1443 274 183 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4311 4343 7884 7916 4311
Size: 32 3541 32 4319
Fragments arranged by size:
4319 3541 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2216 1316
Size: 900 7024
XbaI T'CTAG_A
Cuts at: 5260 5260
Size: 7924
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2786 3481 3490 3670 2786
Size: 695 9 180 7040
Fragments arranged by size:
7040 695 180 9
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7924
XmaI C'CCGG_G
Cuts at: 1870 2960 3096 3162 4578 5000 5026 5249 1870
Size: 1090 136 66 1416 422 26 223 4545
Fragments arranged by size:
4545 1416 1090 422 223 136 66 26
XmnI GAAnn'nnTTC
Cuts at: 6053 6053
Size: 7924
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5732 276
Size: 53 83 186 936 4198 2468
Fragments arranged by size:
4198 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV
FspAI NsiI PacI PspXI PsrI RsrII SapI SexAI
SfiI SwaI