Restriction map for
pFN21AA1847
(Circular) (Six-base) MAPSORT of: pFN21AA1847.seq Check: 7020 from: 1 to: 6238
pFN21AA1847 6238 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2050 2339 2593 2050
Size: 289 254 5695
Fragments arranged by size:
5695 289 254
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4048 278
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3581 1937
Size: 1644 4594
Acc65I G'GTAC_C
Cuts at: 3559 3559
Size: 6238
AclI AA'CG_TT
Cuts at: 1563 4365 4738 1563
Size: 2802 373 3063
Fragments arranged by size:
3063 2802 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2153 2555 4301 5685 5953 1632
Size: 521 402 1746 1384 268 1917
Fragments arranged by size:
1917 1746 1384 521 402 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6041
AflIII A'CryG_T
Cuts at: 1203 3273 5137 6114 1203
Size: 2070 1864 977 1327
Fragments arranged by size:
2070 1864 1327 977
AgeI A'CCGG_T
Cuts at: 5069 5069
Size: 6238
AhdI GACnn_n'nnGTC
Cuts at: 4967 4967
Size: 6238
AleI CACnn'nnGTG
Cuts at: 1166 2723 1166
Size: 1557 4681
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6238
AlwNI CAG_nnn'CTG
Cuts at: 2342 2427 2891 3981 5553 2342
Size: 85 464 1090 1572 3027
Fragments arranged by size:
3027 1572 1090 464 85
ApaI G_GGCC'C
Cuts at: 3232 3307 3232
Size: 75 6163
ApaLI G'TGCA_C
Cuts at: 2936 4295 5451 2936
Size: 1359 1156 3723
Fragments arranged by size:
3723 1359 1156
ApoI r'AATT_y
Cuts at: 1493 3547 3773 3925 6034 1493
Size: 2054 226 152 2109 1697
Fragments arranged by size:
2109 2054 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4792 160
Size: 872 3760 1606
Fragments arranged by size:
3760 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
AvaI C'yCGr_G
Cuts at: 1870 1943 2215 3051 3563 1870
Size: 73 272 836 512 4545
Fragments arranged by size:
4545 836 512 272 73
BamHI G'GATC_C
Cuts at: 3568 3935 3568
Size: 367 5871
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2278 3116 3135 3559
Size: 325 217 627 291 200 838 19 424
Cuts at: 3559 5014 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 838 627 424 325 291 217
200 19
BanII G_rGCy'C
Cuts at: 1391 2142 2151 2654 3030 3232 3307 3448 3475
Size: 751 9 503 376 202 75 141 27
Cuts at: 3475 3557 1391
Size: 82 4072
Fragments arranged by size:
4072 751 503 376 202 141 82 75
27 9
BbeI G_GCGC'C
Cuts at: 2082 2082
Size: 6238
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5267
BbvCI CC'TCA_GC
Cuts at: 2768 2768
Size: 6238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4429 4463 1903
Size: 34 2492 34 3678
Fragments arranged by size:
3678 2492 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4130 5340 4130
Size: 1210 5028
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6238
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3245 3302 4849
Size: 107 122 71 631 605 1573 57 1547
Cuts at: 4849 136
Size: 1525
Fragments arranged by size:
1573 1547 1525 631 605 122 107 71
57
BglII A'GATC_T
Cuts at: 6233 6233
Size: 6238
BlpI GC'TnA_GC
Cuts at: 3655 3655
Size: 6238
Bme1580I G_kGCm'C
Cuts at: 1790 2435 2546 2940 3232 3307 4299 5455 1790
Size: 645 111 394 292 75 992 1156 2573
Fragments arranged by size:
2573 1156 992 645 394 292 111 75
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6238
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3969 4927 442
Size: 416 53 83 399 24 2552 958 1753
Fragments arranged by size:
2552 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6238
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2503 2851 2503
Size: 348 5890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2459 2576 2845 2941 4898 1239
Size: 803 417 117 269 96 1957 2579
Fragments arranged by size:
2579 1957 803 417 269 117 96
Bpu10I CC'TnA_GC
Cuts at: 2648 2768 3308 3474 2648
Size: 120 540 166 5412
Fragments arranged by size:
5412 540 166 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4363 5228 5526 4363
Size: 865 298 5075
Fragments arranged by size:
5075 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2303 2614 3074 4901 882
Size: 617 804 311 460 1827 2219
Fragments arranged by size:
2219 1827 804 617 460 311
BsaAI yAC'GTr
Cuts at: 493 1204 4029 493
Size: 711 2825 2702
Fragments arranged by size:
2825 2702 711
BsaBI GATnn'nnATC
Cuts at: 6232 6232
Size: 6238
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 3413 4045 4427
Size: 53 83 186 936 546 1334 632 382
Cuts at: 4427 5062 275
Size: 635 1451
Fragments arranged by size:
1451 1334 936 635 632 546 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2300 4671 5069 5343 5490 5901 2300
Size: 2371 398 274 147 411 2637
Fragments arranged by size:
2637 2371 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2408 2438 2549 2579 2636
Size: 30 165 30 1005 30 111 30 57
Cuts at: 2636 2666 1178
Size: 30 4750
Fragments arranged by size:
4750 1005 165 111 57 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2375 2954 3165 3274 3277 3347 1186
Size: 841 348 579 211 109 3 70 4077
Fragments arranged by size:
4077 841 579 348 211 109 70 3
BseYI C'CCAG_C
Cuts at: 2928 3169 3250 3351 3420 3447 3457 5441 5937
Size: 241 81 101 69 27 10 1984 496
Cuts at: 5937 2928
Size: 3229
Fragments arranged by size:
3229 1984 496 241 101 81 69 27
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5665
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3688 4449 5477 1540
Size: 275 186 1687 761 1028 2301
Fragments arranged by size:
2301 1687 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2142 2151 2654 2940 4299 4384 5455 2142
Size: 9 503 286 1359 85 1071 2925
Fragments arranged by size:
2925 1359 1071 503 286 85 9
BsmI GAATG_Cn'
Cuts at: 3749 3842 3749
Size: 93 6145
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2151 2435 2546 2654 2940 3030
Size: 399 352 9 284 111 108 286 90
Cuts at: 3030 3232 3307 3448 3475 3557 4299 4384 5455
Size: 202 75 141 27 82 742 85 1071
Cuts at: 5455 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 352 286 284 202
141 111 108 90 85 82 75 27
9
BspEI T'CCGG_A
Cuts at: 2300 5901 2300
Size: 3601 2637
BspHI T'CATG_A
Cuts at: 4127 5857 4127
Size: 1730 4508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2050 2118 2175 2339 2577 2593 2915 3593
Size: 1206 68 57 164 238 16 322 678
Cuts at: 3593 844
Size: 3489
Fragments arranged by size:
3489 1206 678 322 238 164 68 57
16
BsrBI CCG'CTC
Cuts at: 1394 1823 4125 1394
Size: 429 2302 3507
Fragments arranged by size:
3507 2302 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4733 4907 65
Size: 1183 3485 174 1396
Fragments arranged by size:
3485 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2081 2663 3399 3482 4882 5069 1700
Size: 110 271 582 736 83 1400 187 2869
Fragments arranged by size:
2869 1400 736 582 271 187 110 83
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6238
BssHII G'CGCG_C
Cuts at: 1925 2409 1925
Size: 484 5754
BssSI C'ACGA_G
Cuts at: 4298 5310 4298
Size: 1012 5226
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6238
BstBI TT'CG_AA
Cuts at: 5042 5042
Size: 6238
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6238
BstXI CCAn_nnnn'nTGG
Cuts at: 2461 2878 2461
Size: 417 5821
BstYI r'GATC_y
Cuts at: 1972 3568 3935 4320 4337 5789 5875 5887 6233
Size: 1596 367 385 17 1452 86 12 346
Cuts at: 6233 1972
Size: 1977
Fragments arranged by size:
1977 1596 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2293 2454 2596 2778 513
Size: 552 939 289 161 142 182 3973
Fragments arranged by size:
3973 939 552 289 182 161 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5499
Fragments arranged by size:
5499 683 56
BtsI GCAGTG_nn'
Cuts at: 2807 3754 4548 4568 2807
Size: 947 794 20 4477
Fragments arranged by size:
4477 947 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6203
DraI TTT'AAA
Cuts at: 3542 3542
Size: 6238
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5245 809
Size: 507 3929 1802
Fragments arranged by size:
3929 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2679 3397
Size: 54 1425 50 275 18 363 486 718
Cuts at: 3397 3685 4574 6203 8
Size: 288 889 1629 43
Fragments arranged by size:
1629 1425 889 718 486 363 288 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3685 1537
Size: 275 1873 4090
Fragments arranged by size:
4090 1873 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2174 2202 2361 2379 2466 3139 3326 4168
Size: 821 28 159 18 87 673 187 842
Cuts at: 4168 1353
Size: 3423
Fragments arranged by size:
3423 842 821 673 187 159 87 28
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2492 4825 5199 5345 387
Size: 1064 138 903 2333 374 146 1280
Fragments arranged by size:
2333 1280 1064 903 374 146 138
EcoICRI GAG'CTC
Cuts at: 2140 2149 2652 2140
Size: 9 503 5726
Fragments arranged by size:
5726 503 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2153 2459 2555 2576 2845 2941
Size: 393 410 111 306 96 21 269 96
Cuts at: 2941 4301 4898 5685 5953 1239
Size: 1360 597 787 268 1524
Fragments arranged by size:
1524 1360 787 597 410 393 306 269
268 111 96 96 21
EcoNI CCTnn'n_nnAGG
Cuts at: 3491 3491
Size: 6238
EcoO109I rG'GnC_Cy
Cuts at: 2128 2638 2904 3228 3229 3304 2128
Size: 510 266 324 1 75 5062
Fragments arranged by size:
5062 510 324 266 75 1
EcoRI G'AATT_C
Cuts at: 3547 3925 3547
Size: 378 5860
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2587 2587
Size: 6238
FspI TGC'GCA
Cuts at: 2049 2813 4744 2049
Size: 764 1931 3543
Fragments arranged by size:
3543 1931 764
HaeII r_GCGC'y
Cuts at: 1678 2082 2215 3454 5385 1678
Size: 404 133 1239 1931 2531
Fragments arranged by size:
2531 1931 1239 404 133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2636 2668 4879 4911 4953
Size: 32 135 32 1064 32 2211 32 42
Cuts at: 4953 4985 1373
Size: 32 2626
Fragments arranged by size:
2626 2211 1064 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3582 3828 669
Size: 988 281 1644 246 3079
Fragments arranged by size:
3079 1644 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6238
HpaI GTT'AAC
Cuts at: 3828 3828
Size: 6238
KasI G'GCGC_C
Cuts at: 2078 2078
Size: 6238
KpnI G_GTAC'C
Cuts at: 3563 3563
Size: 6238
MfeI C'AATT_G
Cuts at: 3837 3837
Size: 6238
MluI A'CGCG_T
Cuts at: 6114 6114
Size: 6238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2856 5352 5536 1305
Size: 1551 2496 184 2007
Fragments arranged by size:
2496 2007 1551 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2681 10
Size: 54 1425 343 849 3567
Fragments arranged by size:
3567 1425 849 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2459 2691 2723 2972 4196 4555 4714
Size: 648 1293 232 32 249 1224 359 159
Cuts at: 4714 518
Size: 2042
Fragments arranged by size:
2042 1293 1224 648 359 249 232 159
32
MspA1I CmG'CkG
Cuts at: 1606 2053 2119 2288 3254 3355 3424 3457 3654
Size: 447 66 169 966 101 69 33 197
Cuts at: 3654 4331 5479 5724 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 966 677 447 245 197 169
101 69 66 33
NaeI GCC'GGC
Cuts at: 2083 2665 3401 3484 2083
Size: 582 736 83 4837
Fragments arranged by size:
4837 736 582 83
NarI GG'CG_CC
Cuts at: 2079 2079
Size: 6238
NcoI C'CATG_G
Cuts at: 513 1065 2004 2454 2596 513
Size: 552 939 450 142 4155
Fragments arranged by size:
4155 939 552 450 142
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6238
NgoMIV G'CCGG_C
Cuts at: 2081 2663 3399 3482 2081
Size: 582 736 83 4837
Fragments arranged by size:
4837 736 582 83
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6238
NotI GC'GGCC_GC
Cuts at: 3685 3685
Size: 6238
NspI r_CATG'y
Cuts at: 1132 1229 3277 3596 5141 1132
Size: 97 2048 319 1545 2229
Fragments arranged by size:
2229 2048 1545 319 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6238
PciI A'CATG_T
Cuts at: 3273 5137 3273
Size: 1864 4374
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3040 3076 1108
Size: 1932 36 4270
Fragments arranged by size:
4270 1932 36
PfoI T'CCnGG_A
Cuts at: 2250 2553 2634 3372 2250
Size: 303 81 738 5116
Fragments arranged by size:
5116 738 303 81
PmeI GTTT'AAAC
Cuts at: 3542 3542
Size: 6238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4307 4339 5820 5852 4307
Size: 32 1481 32 4693
Fragments arranged by size:
4693 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2128 2638 2904 2128
Size: 510 266 5462
Fragments arranged by size:
5462 510 266
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6238
PsiI TTA'TAA
Cuts at: 3808 3808
Size: 6238
PspOMI G'GGCC_C
Cuts at: 3228 3303 3228
Size: 75 6163
PspXI vC'TCGA_Gb
Cuts at: 3051 3051
Size: 6238
PstI C_TGCA'G
Cuts at: 830 2058 2124 2588 2661 2912 3090 3429 3492
Size: 1228 66 464 73 251 178 339 63
Cuts at: 3492 3590 830
Size: 98 3478
Fragments arranged by size:
3478 1228 464 339 251 178 98 73
66 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6238
PvuII CAG'CTG
Cuts at: 2053 2119 3424 3457 2053
Size: 66 1305 33 4834
Fragments arranged by size:
4834 1305 66 33
SacI G_AGCT'C
Cuts at: 2142 2151 2654 2142
Size: 9 503 5726
Fragments arranged by size:
5726 503 9
SalI G'TCGA_C
Cuts at: 1936 3580 1936
Size: 1644 4594
SapI GCTCTTCn'nnn_
Cuts at: 2174 2202 2379 3326 2174
Size: 28 177 947 5086
Fragments arranged by size:
5086 947 177 28
SbfI CC_TGCA'GG
Cuts at: 2661 3590 2661
Size: 929 5309
ScaI AGT'ACT
Cuts at: 4486 6198 4486
Size: 1712 4526
SfcI C'TryA_G
Cuts at: 826 1045 2054 2120 2419 2584 2657 2908 3086
Size: 219 1009 66 299 165 73 251 178
Cuts at: 3086 3425 3488 3586 4721 5402 5593 826
Size: 339 63 98 1135 681 191 1471
Fragments arranged by size:
1471 1135 1009 681 339 299 251 219
191 178 165 98 73 66 63
SfoI GGC'GCC
Cuts at: 2080 2080
Size: 6238
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6238
SmaI CCC'GGG
Cuts at: 1872 2217 3565 1872
Size: 345 1348 4545
Fragments arranged by size:
4545 1348 345
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3051 4342 5243 5505 820
Size: 197 926 1108 1291 901 262 1553
Fragments arranged by size:
1553 1291 1108 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4029 493
Size: 3536 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6238
SphI G_CATG'C
Cuts at: 1132 3596 1132
Size: 2464 3774
SrfI GCCC'GGGC
Cuts at: 2217 2217
Size: 6238
SspI AAT'ATT
Cuts at: 5 52 4162 5
Size: 47 4110 2081
Fragments arranged by size:
4110 2081 47
StuI AGG'CCT
Cuts at: 1517 3157 1517
Size: 1640 4598
StyI C'CwwG_G
Cuts at: 513 1065 2004 2073 2454 2596 3677 513
Size: 552 939 69 381 142 1081 3074
Fragments arranged by size:
3074 1081 939 552 381 142 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3530 4265 4282 4435 4620 5065 325
Size: 1255 1950 735 17 153 185 445 1498
Fragments arranged by size:
1950 1498 1255 735 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4484 6196 96
Size: 274 80 33 51 1443 2507 1712 138
Fragments arranged by size:
2507 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2549 2581 6198 6230 2549
Size: 32 3617 32 2557
Fragments arranged by size:
3617 2557 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6238
XbaI T'CTAG_A
Cuts at: 3574 3574
Size: 6238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3037 3037
Size: 6238
XhoI C'TCGA_G
Cuts at: 1943 3051 1943
Size: 1108 5130
XmaI C'CCGG_G
Cuts at: 1870 2215 3563 1870
Size: 345 1348 4545
Fragments arranged by size:
4545 1348 345
XmnI GAAnn'nnTTC
Cuts at: 4367 4367
Size: 6238
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4046 276
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI
PacI PmlI PsrI RsrII SacII SanDI SexAI SfiI
SwaI