Restriction map for
pFN21AA1839
(Circular) (Six-base) MAPSORT of: pFN21AA1839.seq Check: 8580 from: 1 to: 6256
pFN21AA1839 6256 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2112 2112
Size: 6256
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4066 278
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3599 1937
Size: 1662 4594
Acc65I G'GTAC_C
Cuts at: 3577 3577
Size: 6256
AclI AA'CG_TT
Cuts at: 1563 4383 4756 1563
Size: 2820 373 3063
Fragments arranged by size:
3063 2820 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2165 2325 2786 2907 3084 3144 4319 5703
Size: 533 160 461 121 177 60 1175 1384
Cuts at: 5703 5971 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1175 533 461 268 177 160
121 60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6059
AflIII A'CryG_T
Cuts at: 1203 3292 5155 6132 1203
Size: 2089 1863 977 1327
Fragments arranged by size:
2089 1863 1327 977
AgeI A'CCGG_T
Cuts at: 5087 5087
Size: 6256
AhdI GACnn_n'nnGTC
Cuts at: 4985 4985
Size: 6256
AleI CACnn'nnGTG
Cuts at: 1166 2881 1166
Size: 1715 4541
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2043 1184
Size: 859 5397
AlwNI CAG_nnn'CTG
Cuts at: 2348 3999 5571 2348
Size: 1651 1572 3033
Fragments arranged by size:
3033 1651 1572
ApaLI G'TGCA_C
Cuts at: 4313 5469 4313
Size: 1156 5100
ApoI r'AATT_y
Cuts at: 1493 2297 2464 2584 2969 3198 3267 3280 3565
Size: 804 167 120 385 229 69 13 285
Cuts at: 3565 3791 3943 6052 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 804 385 285 229 226 167
152 120 69 13
AseI AT'TA_AT
Cuts at: 160 1032 2783 4810 160
Size: 872 1751 2027 1606
Fragments arranged by size:
2027 1751 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2073 3581 1870
Size: 73 72 58 1508 4545
Fragments arranged by size:
4545 1508 73 72 58
BamHI G'GATC_C
Cuts at: 3586 3953 3586
Size: 367 5889
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2100 3577 5032 618
Size: 325 217 627 313 1477 1455 1842
Fragments arranged by size:
1842 1477 1455 627 325 313 217
BanII G_rGCy'C
Cuts at: 1391 2054 2161 3575 1391
Size: 663 107 1414 4072
Fragments arranged by size:
4072 1414 663 107
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2640 3147 928
Size: 971 741 507 4037
Fragments arranged by size:
4037 971 741 507
BbvCI CC'TCA_GC
Cuts at: 2124 2676 2124
Size: 552 5704
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4447 4481 1903
Size: 34 2510 34 3678
Fragments arranged by size:
3678 2510 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4148 5358 4148
Size: 1210 5046
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6256
BfrBI ATG'CAT
Cuts at: 3074 3074
Size: 6256
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4867 136
Size: 107 122 71 631 605 3195 1525
Fragments arranged by size:
3195 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6251 6251
Size: 6256
BlpI GC'TnA_GC
Cuts at: 2112 3673 2112
Size: 1561 4695
Bme1580I G_kGCm'C
Cuts at: 1790 4317 5473 1790
Size: 2527 1156 2573
Fragments arranged by size:
2573 2527 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6256
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3987 4945 442
Size: 416 53 83 399 24 2570 958 1753
Fragments arranged by size:
2570 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4916 1239
Size: 3677 2579
Bpu10I CC'TnA_GC
Cuts at: 2124 2676 2124
Size: 552 5704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2459 4381 5246 5544 2459
Size: 1922 865 298 3171
Fragments arranged by size:
3171 1922 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4919 882
Size: 617 3420 2219
Fragments arranged by size:
3420 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4047 493
Size: 711 2843 2702
Fragments arranged by size:
2843 2702 711
BsaBI GATnn'nnATC
Cuts at: 6250 6250
Size: 6256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4063 4445 5080 275
Size: 53 83 186 936 2530 382 635 1451
Fragments arranged by size:
2530 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4689 5087 5361 5508 5919 4689
Size: 398 274 147 411 5026
Fragments arranged by size:
5026 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6031
Fragments arranged by size:
6031 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2044 2047 2141 2664 3143 1186
Size: 858 3 94 523 479 4299
Fragments arranged by size:
4299 858 523 479 94 3
BseYI C'CCAG_C
Cuts at: 5459 5955 5459
Size: 496 5760
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5683
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2083 3706 4467 5495 1540
Size: 275 186 82 1623 761 1028 2301
Fragments arranged by size:
2301 1623 1028 761 275 186 82
BsiHKAI G_wGCw'C
Cuts at: 2054 4317 4402 5473 2054
Size: 2263 85 1071 2837
Fragments arranged by size:
2837 2263 1071 85
BsmI GAATG_Cn'
Cuts at: 3767 3860 3767
Size: 93 6163
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2161 3575 4317 4402 5473 1391
Size: 399 264 107 1414 742 85 1071 2174
Fragments arranged by size:
2174 1414 1071 742 399 264 107 85
BspEI T'CCGG_A
Cuts at: 5919 5919
Size: 6256
BspHI T'CATG_A
Cuts at: 3046 4145 5875 3046
Size: 1099 1730 3427
Fragments arranged by size:
3427 1730 1099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 3064 3611 844
Size: 1268 952 547 3489
Fragments arranged by size:
3489 1268 952 547
BsrBI CCG'CTC
Cuts at: 1394 1823 4143 1394
Size: 429 2320 3507
Fragments arranged by size:
3507 2320 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2645 4751 4925 65
Size: 1183 1397 2106 174 1396
Fragments arranged by size:
2106 1397 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2107 4900 5087 1700
Size: 110 297 2793 187 2869
Fragments arranged by size:
2869 2793 297 187 110
BsrGI T'GTAC_A
Cuts at: 96 2554 96
Size: 2458 3798
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6256
BssSI C'ACGA_G
Cuts at: 2047 4316 5328 2047
Size: 2269 1012 2975
Fragments arranged by size:
2975 2269 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6256
BstBI TT'CG_AA
Cuts at: 5060 5060
Size: 6256
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6256
BstYI r'GATC_y
Cuts at: 1972 3529 3586 3953 4338 4355 5807 5893 5905
Size: 1557 57 367 385 17 1452 86 12
Cuts at: 5905 6251 1972
Size: 346 1977
Fragments arranged by size:
1977 1557 1452 385 367 346 86 57
17 12
Bsu36I CC'TnA_GG
Cuts at: 2685 3534 2685
Size: 849 5407
BtgI C'CryG_G
Cuts at: 513 1065 2004 2443 3489 513
Size: 552 939 439 1046 3280
Fragments arranged by size:
3280 1046 939 552 439
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5517
Fragments arranged by size:
5517 683 56
BtsI GCAGTG_nn'
Cuts at: 3772 4566 4586 3772
Size: 794 20 5442
Fragments arranged by size:
5442 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2581 2616 529
Size: 35 2017 35 4169
Fragments arranged by size:
4169 2017 35 35
DraI TTT'AAA
Cuts at: 2463 3560 2463
Size: 1097 5159
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5263 809
Size: 507 3947 1802
Fragments arranged by size:
3947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3703 4592 6221
Size: 54 1425 50 275 18 1873 889 1629
Cuts at: 6221 8
Size: 43
Fragments arranged by size:
1873 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3703 1537
Size: 275 1891 4090
Fragments arranged by size:
4090 1891 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2664 4186 1353
Size: 1311 1522 3423
Fragments arranged by size:
3423 1522 1311
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4843 5217 5363 387
Size: 1064 138 3254 374 146 1280
Fragments arranged by size:
3254 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2052 2052
Size: 6256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2325 2786 2907 3084 3144 4319
Size: 393 533 160 461 121 177 60 1175
Cuts at: 4319 4916 5703 5971 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1175 787 597 533 461 393 268
177 160 121 60
EcoNI CCTnn'n_nnAGG
Cuts at: 3537 3537
Size: 6256
EcoO109I rG'GnC_Cy
Cuts at: 2173 2560 3307 2173
Size: 387 747 5122
Fragments arranged by size:
5122 747 387
EcoRI G'AATT_C
Cuts at: 2969 3565 3943 2969
Size: 596 378 5282
Fragments arranged by size:
5282 596 378
EcoRV GAT'ATC
Cuts at: 2034 2034
Size: 6256
FspI TGC'GCA
Cuts at: 4762 4762
Size: 6256
HaeII r_GCGC'y
Cuts at: 1678 2836 5403 1678
Size: 1158 2567 2531
Fragments arranged by size:
2567 2531 1158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2705 2737 3329 3361 4897
Size: 32 135 32 1133 32 592 32 1536
Cuts at: 4897 4929 4971 5003 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1536 1133 592 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3337 3600 3846 669
Size: 988 281 1399 263 246 3079
Fragments arranged by size:
3079 1399 988 281 263 246
HindIII A'AGCT_T
Cuts at: 748 3408 748
Size: 2660 3596
HpaI GTT'AAC
Cuts at: 3846 3846
Size: 6256
KpnI G_GTAC'C
Cuts at: 3581 3581
Size: 6256
MfeI C'AATT_G
Cuts at: 3855 3855
Size: 6256
MluI A'CGCG_T
Cuts at: 6132 6132
Size: 6256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5370 5554 1305
Size: 4065 184 2007
Fragments arranged by size:
4065 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4434
Fragments arranged by size:
4434 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2881 4214 4573 4732 518
Size: 648 1715 1333 359 159 2042
Fragments arranged by size:
2042 1715 1333 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2996 3358 3672 4349 5497 5742 1606
Size: 1390 362 314 677 1148 245 2120
Fragments arranged by size:
2120 1390 1148 677 362 314 245
NaeI GCC'GGC
Cuts at: 2109 2109
Size: 6256
NcoI C'CATG_G
Cuts at: 513 1065 2004 2443 3489 513
Size: 552 939 439 1046 3280
Fragments arranged by size:
3280 1046 939 552 439
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6256
NgoMIV G'CCGG_C
Cuts at: 2107 2107
Size: 6256
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6256
NotI GC'GGCC_GC
Cuts at: 3703 3703
Size: 6256
NsiI A_TGCA'T
Cuts at: 3076 3076
Size: 6256
NspI r_CATG'y
Cuts at: 1132 1229 2654 2992 3296 3614 5159 1132
Size: 97 1425 338 304 318 1545 2229
Fragments arranged by size:
2229 1545 1425 338 318 304 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6256
PciI A'CATG_T
Cuts at: 3292 5155 3292
Size: 1863 4393
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2444 1108
Size: 1336 4920
PmeI GTTT'AAAC
Cuts at: 3560 3560
Size: 6256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 5838 5870 4325
Size: 32 1481 32 4711
Fragments arranged by size:
4711 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2173 2560 3307 2173
Size: 387 747 5122
Fragments arranged by size:
5122 747 387
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6256
PsiI TTA'TAA
Cuts at: 3826 3826
Size: 6256
PstI C_TGCA'G
Cuts at: 830 3608 830
Size: 2778 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6256
PvuII CAG'CTG
Cuts at: 2996 2996
Size: 6256
SacI G_AGCT'C
Cuts at: 2054 2054
Size: 6256
SalI G'TCGA_C
Cuts at: 1936 3598 1936
Size: 1662 4594
SbfI CC_TGCA'GG
Cuts at: 3608 3608
Size: 6256
ScaI AGT'ACT
Cuts at: 2150 4504 6216 2150
Size: 2354 1712 2190
Fragments arranged by size:
2354 2190 1712
SfcI C'TryA_G
Cuts at: 826 1045 2949 3262 3604 4739 5420 5611 826
Size: 219 1904 313 342 1135 681 191 1471
Fragments arranged by size:
1904 1471 1135 681 342 313 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6256
SmaI CCC'GGG
Cuts at: 1872 3583 1872
Size: 1711 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2474 4360 5261 5523 820
Size: 197 926 531 1886 901 262 1553
Fragments arranged by size:
1886 1553 926 901 531 262 197
SnaBI TAC'GTA
Cuts at: 493 4047 493
Size: 3554 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6256
SphI G_CATG'C
Cuts at: 1132 2654 3614 1132
Size: 1522 960 3774
Fragments arranged by size:
3774 1522 960
SspI AAT'ATT
Cuts at: 5 52 4180 5
Size: 47 4128 2081
Fragments arranged by size:
4128 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6256
StyI C'CwwG_G
Cuts at: 513 1065 2004 2443 2529 3489 3695 513
Size: 552 939 439 86 960 206 3074
Fragments arranged by size:
3074 960 939 552 439 206 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2097 2680 2778 4283 4300 4453 4638
Size: 1255 517 583 98 1505 17 153 185
Cuts at: 4638 5083 325
Size: 445 1498
Fragments arranged by size:
1505 1498 1255 583 517 445 185 153
98 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2148 2554 4502
Size: 274 80 33 51 1443 171 406 1948
Cuts at: 4502 6214 96
Size: 1712 138
Fragments arranged by size:
1948 1712 1443 406 274 171 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6216 6248 6216
Size: 32 6224
Tth111I GACn'n_nGTC
Cuts at: 1316 2375 1316
Size: 1059 5197
XbaI T'CTAG_A
Cuts at: 3173 3203 3592 3173
Size: 30 389 5837
Fragments arranged by size:
5837 389 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3112 3112
Size: 6256
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6256
XmaI C'CCGG_G
Cuts at: 1870 3581 1870
Size: 1711 4545
XmnI GAAnn'nnTTC
Cuts at: 2150 4385 2150
Size: 2235 4021
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4064 276
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I DraIII FalI FseI FspAI
KasI NarI NruI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI