Restriction map for
pFN21AA1826
(Circular) (Six-base) MAPSORT of: pFN21AA1826.seq Check: 3017 from: 1 to: 5740
pFN21AA1826 5740 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2396 2396
Size: 5740
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3550 278
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3083 1937
Size: 1146 4594
Acc65I G'GTAC_C
Cuts at: 2220 3061 2220
Size: 841 4899
AclI AA'CG_TT
Cuts at: 1563 3867 4240 1563
Size: 2304 373 3063
Fragments arranged by size:
3063 2304 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2349 2738 3803 5187 5455 1632
Size: 717 389 1065 1384 268 1917
Fragments arranged by size:
1917 1384 1065 717 389 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5543
AflIII A'CryG_T
Cuts at: 1203 4639 5616 1203
Size: 3436 977 1327
Fragments arranged by size:
3436 1327 977
AgeI A'CCGG_T
Cuts at: 4571 4571
Size: 5740
AhdI GACnn_n'nnGTC
Cuts at: 4469 4469
Size: 5740
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5740
AlwNI CAG_nnn'CTG
Cuts at: 3483 5055 3483
Size: 1572 4168
ApaLI G'TGCA_C
Cuts at: 3797 4953 3797
Size: 1156 4584
ApoI r'AATT_y
Cuts at: 1493 2368 2465 3015 3049 3275 3427 5536 1493
Size: 875 97 550 34 226 152 2109 1697
Fragments arranged by size:
2109 1697 875 550 226 152 97 34
AseI AT'TA_AT
Cuts at: 160 1032 2124 4294 160
Size: 872 1092 2170 1606
Fragments arranged by size:
2170 1606 1092 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
AvaI C'yCGr_G
Cuts at: 1870 1943 3065 1870
Size: 73 1122 4545
Fragments arranged by size:
4545 1122 73
BamHI G'GATC_C
Cuts at: 2411 2449 3070 3437 2411
Size: 38 621 367 4714
Fragments arranged by size:
4714 621 367 38
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2220 3061 4516 618
Size: 325 217 627 433 841 1455 1842
Fragments arranged by size:
1842 1455 841 627 433 325 217
BanII G_rGCy'C
Cuts at: 1391 3059 1391
Size: 1668 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4769
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3931 3965 1903
Size: 34 1994 34 3678
Fragments arranged by size:
3678 1994 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3632 4842 3632
Size: 1210 4530
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5740
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4351 136
Size: 107 122 71 631 605 2679 1525
Fragments arranged by size:
2679 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2588 5735 2588
Size: 3147 2593
BlpI GC'TnA_GC
Cuts at: 3157 3157
Size: 5740
Bme1580I G_kGCm'C
Cuts at: 1790 3801 4957 1790
Size: 2011 1156 2573
Fragments arranged by size:
2573 2011 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5740
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3471 4429 442
Size: 416 53 83 399 24 2054 958 1753
Fragments arranged by size:
2054 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4400 1239
Size: 3161 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2924 3865 4730 5028 2924
Size: 941 865 298 3636
Fragments arranged by size:
3636 941 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4403 882
Size: 617 2904 2219
Fragments arranged by size:
2904 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3531 493
Size: 711 2327 2702
Fragments arranged by size:
2702 2327 711
BsaBI GATnn'nnATC
Cuts at: 5734 5734
Size: 5740
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3547 3929 4564 275
Size: 53 83 186 936 2014 382 635 1451
Fragments arranged by size:
2014 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4173 4571 4845 4992 5403 4173
Size: 398 274 147 411 4510
Fragments arranged by size:
4510 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5515
Fragments arranged by size:
5515 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2293 2492 2495 1186
Size: 1107 199 3 4431
Fragments arranged by size:
4431 1107 199 3
BseYI C'CCAG_C
Cuts at: 4943 5439 4943
Size: 496 5244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5167
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3190 3951 4979 1540
Size: 275 186 1189 761 1028 2301
Fragments arranged by size:
2301 1189 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2239 3801 3886 4957 2239
Size: 1562 85 1071 3022
Fragments arranged by size:
3022 1562 1071 85
BsmI GAATG_Cn'
Cuts at: 2176 3251 3344 2176
Size: 1075 93 4572
Fragments arranged by size:
4572 1075 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2239 3059 3801 3886 4957 1391
Size: 399 449 820 742 85 1071 2174
Fragments arranged by size:
2174 1071 820 742 449 399 85
BspEI T'CCGG_A
Cuts at: 5403 5403
Size: 5740
BspHI T'CATG_A
Cuts at: 3629 5359 3629
Size: 1730 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2396 2674 3028 3095 844
Size: 1552 278 354 67 3489
Fragments arranged by size:
3489 1552 354 278 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2749 2770 3627 1394
Size: 429 926 21 857 3507
Fragments arranged by size:
3507 926 857 429 21
BsrDI GCAATG_nn'
Cuts at: 65 1248 4235 4409 65
Size: 1183 2987 174 1396
Fragments arranged by size:
2987 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4384 4571 1700
Size: 110 2574 187 2869
Fragments arranged by size:
2869 2574 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5740
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5740
BssSI C'ACGA_G
Cuts at: 3800 4812 3800
Size: 1012 4728
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5740
BstBI TT'CG_AA
Cuts at: 3013 4544 3013
Size: 1531 4209
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5740
BstYI r'GATC_y
Cuts at: 1972 2411 2449 2588 3070 3437 3822 3839 5291
Size: 439 38 139 482 367 385 17 1452
Cuts at: 5291 5377 5389 5735 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 482 439 385 367 346 139
86 38 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5001
Fragments arranged by size:
5001 683 56
BtsI GCAGTG_nn'
Cuts at: 3256 4050 4070 3256
Size: 794 20 4926
Fragments arranged by size:
4926 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5740
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5705
DraI TTT'AAA
Cuts at: 3044 3044
Size: 5740
DraIII CAC_nnn'GTG
Cuts at: 2165 2165
Size: 5740
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4747 809
Size: 507 3431 1802
Fragments arranged by size:
3431 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3187 4076 5705
Size: 54 1425 50 275 18 1357 889 1629
Cuts at: 5705 8
Size: 43
Fragments arranged by size:
1629 1425 1357 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3187 1537
Size: 275 1375 4090
Fragments arranged by size:
4090 1375 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2145 2240 2325 2430 3670 1353
Size: 792 95 85 105 1240 3423
Fragments arranged by size:
3423 1240 792 105 95 85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4327 4701 4847 387
Size: 1064 138 2738 374 146 1280
Fragments arranged by size:
2738 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2349 2738 3803 4400 5187 5455 1239
Size: 393 717 389 1065 597 787 268 1524
Fragments arranged by size:
1524 1065 787 717 597 393 389 268
EcoRI G'AATT_C
Cuts at: 3015 3049 3427 3015
Size: 34 378 5328
Fragments arranged by size:
5328 378 34
EcoRV GAT'ATC
Cuts at: 2665 2665
Size: 5740
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3004 3026 3004
Size: 22 5718
FspI TGC'GCA
Cuts at: 4246 4246
Size: 5740
HaeII r_GCGC'y
Cuts at: 1678 4887 1678
Size: 3209 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2649 2681 2810 2842 4381
Size: 32 135 32 1077 32 129 32 1539
Cuts at: 4381 4413 4455 4487 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1539 1077 135 129 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3084 3330 669
Size: 988 281 1146 246 3079
Fragments arranged by size:
3079 1146 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5740
HpaI GTT'AAC
Cuts at: 3330 3330
Size: 5740
KpnI G_GTAC'C
Cuts at: 2224 3065 2224
Size: 841 4899
MfeI C'AATT_G
Cuts at: 3339 3339
Size: 5740
MluI A'CGCG_T
Cuts at: 5616 5616
Size: 5740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2625 4854 5038 1305
Size: 1320 2229 184 2007
Fragments arranged by size:
2229 2007 1320 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3918
Fragments arranged by size:
3918 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2553 3698 4057 4216 518
Size: 648 1387 1145 359 159 2042
Fragments arranged by size:
2042 1387 1145 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3156 3833 4981 5226 1606
Size: 1550 677 1148 245 2120
Fragments arranged by size:
2120 1550 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5188
NdeI CA'TA_TG
Cuts at: 387 2596 387
Size: 2209 3531
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5740
NotI GC'GGCC_GC
Cuts at: 3187 3187
Size: 5740
NspI r_CATG'y
Cuts at: 1132 1229 3098 4643 1132
Size: 97 1869 1545 2229
Fragments arranged by size:
2229 1869 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5740
PciI A'CATG_T
Cuts at: 4639 4639
Size: 5740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5740
PfoI T'CCnGG_A
Cuts at: 2516 2516
Size: 5740
PmeI GTTT'AAAC
Cuts at: 3044 3044
Size: 5740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 5322 5354 3809
Size: 32 1481 32 4195
Fragments arranged by size:
4195 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5740
PsiI TTA'TAA
Cuts at: 3310 3310
Size: 5740
PstI C_TGCA'G
Cuts at: 830 2685 2778 2943 2973 3092 830
Size: 1855 93 165 30 119 3478
Fragments arranged by size:
3478 1855 165 119 93 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5740
SalI G'TCGA_C
Cuts at: 1936 3082 1936
Size: 1146 4594
SbfI CC_TGCA'GG
Cuts at: 3092 3092
Size: 5740
ScaI AGT'ACT
Cuts at: 3988 5700 3988
Size: 1712 4028
SfcI C'TryA_G
Cuts at: 826 1045 2175 2681 2774 2939 2969 3088 4223
Size: 219 1130 506 93 165 30 119 1135
Cuts at: 4223 4904 5095 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1130 681 506 219 191 165
119 93 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5740
SmaI CCC'GGG
Cuts at: 1872 3067 1872
Size: 1195 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2903 3844 4745 5007 820
Size: 197 926 960 941 901 262 1553
Fragments arranged by size:
1553 960 941 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3531 493
Size: 3038 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5740
SphI G_CATG'C
Cuts at: 1132 3098 1132
Size: 1966 3774
SspI AAT'ATT
Cuts at: 5 52 2623 3664 5
Size: 47 2571 1041 2081
Fragments arranged by size:
2571 2081 1041 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5740
StyI C'CwwG_G
Cuts at: 513 1065 2829 3179 513
Size: 552 1764 350 3074
Fragments arranged by size:
3074 1764 552 350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3767 3784 3937 4122 4567 325
Size: 1255 2187 17 153 185 445 1498
Fragments arranged by size:
2187 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3986 5698 96
Size: 274 80 33 51 1443 2009 1712 138
Fragments arranged by size:
2009 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5700 5732 5700
Size: 32 5708
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5740
XbaI T'CTAG_A
Cuts at: 2585 3076 2585
Size: 491 5249
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2272 2272
Size: 5740
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5740
XmaI C'CCGG_G
Cuts at: 1870 3065 1870
Size: 1195 4545
XmnI GAAnn'nnTTC
Cuts at: 3869 3869
Size: 5740
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3548 276
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI