Restriction map for
pFN21AA1825
(Circular) (Six-base) MAPSORT of: pFN21AA1825.seq Check: 1057 from: 1 to: 8317
pFN21AA1825 8317 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2399 4467 4498 2399
Size: 2068 31 6218
Fragments arranged by size:
6218 2068 31
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6127 278
Size: 53 83 186 936 4591 2468
Fragments arranged by size:
4591 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2913 3045 5214 5313 5660 1937
Size: 976 132 2169 99 347 4594
Fragments arranged by size:
4594 2169 976 347 132 99
Acc65I G'GTAC_C
Cuts at: 3401 5638 3401
Size: 2237 6080
AclI AA'CG_TT
Cuts at: 1563 6444 6817 1563
Size: 4881 373 3063
Fragments arranged by size:
4881 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3233 3914 4580 4640 6380 7764 8032 1632
Size: 1601 681 666 60 1740 1384 268 1917
Fragments arranged by size:
1917 1740 1601 1384 681 666 268 60
AfeI AGC'GCT
Cuts at: 3872 3872
Size: 8317
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8120
AflIII A'CryG_T
Cuts at: 1203 2865 3540 7216 8193 1203
Size: 1662 675 3676 977 1327
Fragments arranged by size:
3676 1662 1327 977 675
AgeI A'CCGG_T
Cuts at: 7148 7148
Size: 8317
AhdI GACnn_n'nnGTC
Cuts at: 7046 7046
Size: 8317
AleI CACnn'nnGTG
Cuts at: 1166 3170 3758 4946 1166
Size: 2004 588 1188 4537
Fragments arranged by size:
4537 2004 1188 588
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2051 2300 3299 4394 4397 1184
Size: 867 249 999 1095 3 5104
Fragments arranged by size:
5104 1095 999 867 249 3
AlwNI CAG_nnn'CTG
Cuts at: 2255 3856 4285 4483 4816 5068 5533 6060 7632
Size: 1601 429 198 333 252 465 527 1572
Cuts at: 7632 2255
Size: 2940
Fragments arranged by size:
2940 1601 1572 527 465 429 333 252
198
ApaI G_GGCC'C
Cuts at: 2050 3715 3973 2050
Size: 1665 258 6394
Fragments arranged by size:
6394 1665 258
ApaLI G'TGCA_C
Cuts at: 2369 3445 5195 6374 7530 2369
Size: 1076 1750 1179 1156 3156
Fragments arranged by size:
3156 1750 1179 1156 1076
ApoI r'AATT_y
Cuts at: 1493 2522 2666 3861 5626 5852 6004 8113 1493
Size: 1029 144 1195 1765 226 152 2109 1697
Fragments arranged by size:
2109 1765 1697 1195 1029 226 152 144
AseI AT'TA_AT
Cuts at: 160 1032 6871 160
Size: 872 5839 1606
Fragments arranged by size:
5839 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8317
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 2353 4041 4107 4392 4692 4826
Size: 73 144 266 1688 66 285 300 134
Cuts at: 4826 5127 5246 5390 5642 1870
Size: 301 119 144 252 4545
Fragments arranged by size:
4545 1688 301 300 285 266 252 144
144 134 119 73 66
BamHI G'GATC_C
Cuts at: 3564 5647 6014 3564
Size: 2083 367 5867
Fragments arranged by size:
5867 2083 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 2193 2243 2437 2695
Size: 325 217 627 248 158 50 194 258
Cuts at: 2695 2775 3004 3164 3401 3752 4598 4613 5638
Size: 80 229 160 237 351 846 15 1025
Cuts at: 5638 7093 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1025 846 627 351 325 258
248 237 229 217 194 160 158 80
50 15
BanII G_rGCy'C
Cuts at: 1391 2050 2469 2772 3093 3558 3715 3935 3973
Size: 659 419 303 321 465 157 220 38
Cuts at: 3973 4054 4400 4764 5246 5636 1391
Size: 81 346 364 482 390 4072
Fragments arranged by size:
4072 659 482 465 419 390 364 346
321 303 220 157 81 38
BbeI G_GCGC'C
Cuts at: 2197 4617 2197
Size: 2420 5897
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2502 2643 3105 928
Size: 971 603 141 462 6140
Fragments arranged by size:
6140 971 603 462 141
BbvCI CC'TCA_GC
Cuts at: 3104 3104
Size: 8317
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3018 3052 6508 6542 1903
Size: 34 1081 34 3456 34 3678
Fragments arranged by size:
3678 3456 1081 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6209 7419 6209
Size: 1210 7107
BclI T'GATC_A
Cuts at: 1548 4239 4923 1548
Size: 2691 684 4942
Fragments arranged by size:
4942 2691 684
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2074 2123
Size: 107 122 71 631 605 337 65 49
Cuts at: 2123 2173 4440 4523 5484 6928 136
Size: 50 2267 83 961 1444 1525
Fragments arranged by size:
2267 1525 1444 961 631 605 337 122
107 83 71 65 50 49
BglII A'GATC_T
Cuts at: 4035 8312 4035
Size: 4277 4040
BlpI GC'TnA_GC
Cuts at: 5734 5734
Size: 8317
Bme1580I G_kGCm'C
Cuts at: 1790 2040 2050 2373 3167 3449 3715 3892 3973
Size: 250 10 323 794 282 266 177 81
Cuts at: 3973 4007 5199 6378 7534 1790
Size: 34 1192 1179 1156 2573
Fragments arranged by size:
2573 1192 1179 1156 794 323 282 266
250 177 81 34 10
BmgBI CAC'GTC
Cuts at: 1318 3016 3151 4609 4633 1318
Size: 1698 135 1458 24 5002
Fragments arranged by size:
5002 1698 1458 135 24
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3674 3994 4865
Size: 416 53 83 399 24 2257 320 871
Cuts at: 4865 6048 7006 442
Size: 1183 958 1753
Fragments arranged by size:
2257 1753 1183 958 871 416 399 320
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8317
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4375 4375
Size: 8317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2576 3113 3350 3461 4151 4439 4720 5045
Size: 1337 537 237 111 690 288 281 325
Cuts at: 5045 5168 6977 1239
Size: 123 1809 2579
Fragments arranged by size:
2579 1809 1337 690 537 325 288 281
237 123 111
Bpu10I CC'TnA_GC
Cuts at: 3104 3477 4662 4709 4805 5204 3104
Size: 373 1185 47 96 399 6217
Fragments arranged by size:
6217 1185 399 373 96 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2525 4127 4163 5099 6442 7307 7605 2525
Size: 1602 36 936 1343 865 298 3237
Fragments arranged by size:
3237 1602 1343 936 865 298 36
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3326 4171 4815 6980 882
Size: 617 1827 845 644 2165 2219
Fragments arranged by size:
2219 2165 1827 845 644 617
BsaAI yAC'GTr
Cuts at: 493 1204 4273 4576 4921 6108 493
Size: 711 3069 303 345 1187 2702
Fragments arranged by size:
3069 2702 1187 711 345 303
BsaBI GATnn'nnATC
Cuts at: 8311 8311
Size: 8317
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2194 4614 6124 6506
Size: 53 83 186 936 661 2420 1510 382
Cuts at: 6506 7141 275
Size: 635 1451
Fragments arranged by size:
2420 1510 1451 936 661 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2877 3254 3561 6750 7148 7422 7569 7980 2877
Size: 377 307 3189 398 274 147 411 3214
Fragments arranged by size:
3214 3189 411 398 377 307 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2441 2471 2957 2987 4041
Size: 30 165 30 1038 30 486 30 1054
Cuts at: 4041 4071 1178
Size: 30 5424
Fragments arranged by size:
5424 1054 1038 486 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4790 5441 5449 1186
Size: 3604 651 8 4054
Fragments arranged by size:
4054 3604 651 8
BseYI C'CCAG_C
Cuts at: 2262 2268 2411 3413 3928 4066 4081 4476 4567
Size: 6 143 1002 515 138 15 395 91
Cuts at: 4567 7520 8016 2262
Size: 2953 496 2563
Fragments arranged by size:
2953 2563 1002 515 496 395 143 138
91 15 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2306 2377 2462 2867 3131 3197 3532
Size: 573 570 71 85 405 264 66 335
Cuts at: 3532 3968 4156 4271 4526 4924 1163
Size: 436 188 115 255 398 4556
Fragments arranged by size:
4556 573 570 436 405 398 335 264
255 188 115 85 71 66
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2983 3468 3795 3960 4757 5569
Size: 275 186 982 485 327 165 797 812
Cuts at: 5569 5767 6528 7556 1540
Size: 198 761 1028 2301
Fragments arranged by size:
2301 1028 982 812 797 761 485 327
275 198 186 165
BsiHKAI G_wGCw'C
Cuts at: 2373 2469 3449 5199 6378 6463 7534 2373
Size: 96 980 1750 1179 85 1071 3156
Fragments arranged by size:
3156 1750 1179 1071 980 96 85
BsmI GAATG_Cn'
Cuts at: 4222 5828 5921 4222
Size: 1606 93 6618
Fragments arranged by size:
6618 1606 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2977 3654 2977
Size: 677 7640
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2050 2373 2469 2772 3093 3167
Size: 399 250 10 323 96 303 321 74
Cuts at: 3167 3449 3558 3715 3892 3935 3973 4007 4054
Size: 282 109 157 177 43 38 34 47
Cuts at: 4054 4400 4764 5199 5246 5636 6378 6463 7534
Size: 346 364 435 47 390 742 85 1071
Cuts at: 7534 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 435 399 390 364 346
323 321 303 282 250 177 157 109
96 85 74 47 47 43 38 34
10
BspEI T'CCGG_A
Cuts at: 2877 3561 7980 2877
Size: 684 4419 3214
Fragments arranged by size:
4419 3214 684
BspHI T'CATG_A
Cuts at: 4236 6206 7936 4236
Size: 1970 1730 4617
Fragments arranged by size:
4617 1970 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2399 4467 4498 5672 844
Size: 1555 2068 31 1174 3489
Fragments arranged by size:
3489 2068 1555 1174 31
BsrBI CCG'CTC
Cuts at: 1394 1823 2985 4177 4258 4666 4678 6204 1394
Size: 429 1162 1192 81 408 12 1526 3507
Fragments arranged by size:
3507 1526 1192 1162 429 408 81 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 2377 2491 2937 5520 6812 6986 65
Size: 1183 1129 114 446 2583 1292 174 1396
Fragments arranged by size:
2583 1396 1292 1183 1129 446 174 114
BsrFI r'CCGG_y
Cuts at: 1700 1810 2122 2172 3217 4341 5078 6961 7148
Size: 110 312 50 1045 1124 737 1883 187
Cuts at: 7148 1700
Size: 2869
Fragments arranged by size:
2869 1883 1124 1045 737 312 187 110
50
BsrGI T'GTAC_A
Cuts at: 96 5160 5274 96
Size: 5064 114 3139
Fragments arranged by size:
5064 3139 114
BssHII G'CGCG_C
Cuts at: 1925 2101 2103 2382 1925
Size: 176 2 279 7860
Fragments arranged by size:
7860 279 176 2
BssSI C'ACGA_G
Cuts at: 2309 2468 3721 3758 5489 6377 7389 2309
Size: 159 1253 37 1731 888 1012 3237
Fragments arranged by size:
3237 1731 1253 1012 888 159 37
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2857 794
Size: 2063 6254
BstBI TT'CG_AA
Cuts at: 2520 7121 2520
Size: 4601 3716
BstEII G'GTnAC_C
Cuts at: 1184 3673 3764 4948 1184
Size: 2489 91 1184 4553
Fragments arranged by size:
4553 2489 1184 91
BstXI CCAn_nnnn'nTGG
Cuts at: 2824 4609 5338 2824
Size: 1785 729 5803
Fragments arranged by size:
5803 1785 729
BstYI r'GATC_y
Cuts at: 1972 2289 2646 2874 3564 4035 4209 4971 5647
Size: 317 357 228 690 471 174 762 676
Cuts at: 5647 6014 6399 6416 7868 7954 7966 8312 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 762 690 676 471 385 367
357 346 317 228 174 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2460 2577 3204 3795 5325 513
Size: 552 939 456 117 627 591 1530 3505
Fragments arranged by size:
3505 1530 939 627 591 552 456 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3355 3505 3937 4855 487
Size: 683 56 2129 150 432 918 3949
Fragments arranged by size:
3949 2129 918 683 432 150 56
BtsI GCAGTG_nn'
Cuts at: 4260 4352 5833 6627 6647 4260
Size: 92 1481 794 20 5930
Fragments arranged by size:
5930 1481 794 92 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8317
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8282
DraI TTT'AAA
Cuts at: 5621 5621
Size: 8317
DraIII CAC_nnn'GTG
Cuts at: 4231 4231
Size: 8317
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3682 7324 809
Size: 507 2366 3642 1802
Fragments arranged by size:
3642 2366 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2165 2980 3501
Size: 54 1425 50 275 18 335 815 521
Cuts at: 3501 3528 3648 3792 3957 4650 5076 5322 5328
Size: 27 120 144 165 693 426 246 6
Cuts at: 5328 5340 5433 5562 5764 6653 8282 8
Size: 12 93 129 202 889 1629 43
Fragments arranged by size:
1629 1425 889 815 693 521 426 335
275 246 202 165 144 129 120 93
54 50 43 27 18 12 6
EagI C'GGCC_G
Cuts at: 1537 1812 2980 3792 3957 5764 1537
Size: 275 1168 812 165 1807 4090
Fragments arranged by size:
4090 1807 1168 812 275 165
EarI CTCTTCn'nnn_
Cuts at: 1353 3302 3356 5096 6247 1353
Size: 1949 54 1740 1151 3423
Fragments arranged by size:
3423 1949 1740 1151 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4478 6904 7278 7424 387
Size: 1064 138 2889 2426 374 146 1280
Fragments arranged by size:
2889 2426 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2467 2467
Size: 8317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2576 3113 3233 3350 3461 3914 4151
Size: 393 944 537 120 117 111 453 237
Cuts at: 4151 4439 4580 4640 4720 5045 5168 6380 6977
Size: 288 141 60 80 325 123 1212 597
Cuts at: 6977 7764 8032 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1212 944 787 597 537 453 393
325 288 268 237 141 123 120 117
111 80 60
EcoNI CCTnn'n_nnAGG
Cuts at: 4792 4808 4792
Size: 16 8301
EcoO109I rG'GnC_Cy
Cuts at: 2046 3409 3712 3969 3970 4126 5530 5592 2046
Size: 1363 303 257 1 156 1404 62 4771
Fragments arranged by size:
4771 1404 1363 303 257 156 62 1
EcoRI G'AATT_C
Cuts at: 2522 5626 6004 2522
Size: 3104 378 4835
Fragments arranged by size:
4835 3104 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2519 2519
Size: 8317
FspI TGC'GCA
Cuts at: 3287 6823 3287
Size: 3536 4781
HaeII r_GCGC'y
Cuts at: 1678 2129 2186 2197 3874 4067 4360 4617 4646
Size: 451 57 11 1677 193 293 257 29
Cuts at: 4646 4982 5558 7464 1678
Size: 336 576 1906 2531
Fragments arranged by size:
2531 1906 1677 576 451 336 293 257
193 57 29 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2498 2530 2957 2989 4041
Size: 32 135 32 926 32 427 32 1052
Cuts at: 4041 4073 6958 6990 7032 7064 1373
Size: 32 2885 32 42 32 2626
Fragments arranged by size:
2885 2626 1052 926 427 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3223 3508 3618 5302 5661 5907
Size: 988 281 1285 285 110 1684 359 246
Cuts at: 5907 669
Size: 3079
Fragments arranged by size:
3079 1684 1285 988 359 285 281 246
110
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8317
HpaI GTT'AAC
Cuts at: 5907 5907
Size: 8317
KasI G'GCGC_C
Cuts at: 2193 4613 2193
Size: 2420 5897
KpnI G_GTAC'C
Cuts at: 3405 5642 3405
Size: 2237 6080
MfeI C'AATT_G
Cuts at: 5916 5916
Size: 8317
MluI A'CGCG_T
Cuts at: 8193 8193
Size: 8317
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2850 7431 7615 1305
Size: 1545 4581 184 2007
Fragments arranged by size:
4581 2007 1545 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3530 4652 5324 5330 5342
Size: 54 1425 343 1698 1122 672 6 12
Cuts at: 5342 5435 10
Size: 93 2892
Fragments arranged by size:
2892 1698 1425 1122 672 343 93 54
12 6
MslI CAynn'nnrTG
Cuts at: 518 1166 2484 2822 3170 3758 4946 5177 5324
Size: 648 1318 338 348 588 1188 231 147
Cuts at: 5324 5330 5336 5501 6275 6634 6793 518
Size: 6 6 165 774 359 159 2042
Fragments arranged by size:
2042 1318 1188 774 648 588 359 348
338 231 165 159 147 6 6
MspA1I CmG'CkG
Cuts at: 1606 2015 2255 2657 3036 3228 3380 3742 4483
Size: 409 240 402 379 192 152 362 741
Cuts at: 4483 4753 4938 5733 6410 7558 7803 1606
Size: 270 185 795 677 1148 245 2120
Fragments arranged by size:
2120 1148 795 741 677 409 402 379
362 270 245 240 192 185 152
NaeI GCC'GGC
Cuts at: 2124 4343 5080 2124
Size: 2219 737 5361
Fragments arranged by size:
5361 2219 737
NarI GG'CG_CC
Cuts at: 2194 4614 2194
Size: 2420 5897
NcoI C'CATG_G
Cuts at: 513 1065 2004 5325 513
Size: 552 939 3321 3505
Fragments arranged by size:
3505 3321 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8317
NgoMIV G'CCGG_C
Cuts at: 2122 4341 5078 2122
Size: 2219 737 5361
Fragments arranged by size:
5361 2219 737
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8317
NotI GC'GGCC_GC
Cuts at: 5764 5764
Size: 8317
NspI r_CATG'y
Cuts at: 1132 1229 2869 3544 4269 4628 5675 7220 1132
Size: 97 1640 675 725 359 1047 1545 2229
Fragments arranged by size:
2229 1640 1545 1047 725 675 359 97
PasI CC'CwG_GG
Cuts at: 1348 3271 1348
Size: 1923 6394
PciI A'CATG_T
Cuts at: 2865 3540 7216 2865
Size: 675 3676 3966
Fragments arranged by size:
3966 3676 675
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4231 1108
Size: 3123 5194
PfoI T'CCnGG_A
Cuts at: 2631 4203 4736 5538 2631
Size: 1572 533 802 5410
Fragments arranged by size:
5410 1572 802 533
PmeI GTTT'AAAC
Cuts at: 5621 5621
Size: 8317
PmlI CAC'GTG
Cuts at: 4273 4921 4273
Size: 648 7669
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6386 6418 7899 7931 6386
Size: 32 1481 32 6772
Fragments arranged by size:
6772 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3409 5530 5592 3409
Size: 2121 62 6134
Fragments arranged by size:
6134 2121 62
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8317
PsiI TTA'TAA
Cuts at: 5887 5887
Size: 8317
PspOMI G'GGCC_C
Cuts at: 2046 3711 3969 2046
Size: 1665 258 6394
Fragments arranged by size:
6394 1665 258
PspXI vC'TCGA_Gb
Cuts at: 4826 4826
Size: 8317
PstI C_TGCA'G
Cuts at: 830 3379 4323 4464 5580 5669 830
Size: 2549 944 141 1116 89 3478
Fragments arranged by size:
3478 2549 1116 944 141 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8317
PvuII CAG'CTG
Cuts at: 2657 3380 3742 4483 2657
Size: 723 362 741 6491
Fragments arranged by size:
6491 741 723 362
RsrII CG'GwC_CG
Cuts at: 3252 3252
Size: 8317
SacI G_AGCT'C
Cuts at: 2469 2469
Size: 8317
SalI G'TCGA_C
Cuts at: 1936 5659 1936
Size: 3723 4594
SanDI GG'GwC_CC
Cuts at: 5592 5592
Size: 8317
SbfI CC_TGCA'GG
Cuts at: 4464 5669 4464
Size: 1205 7112
ScaI AGT'ACT
Cuts at: 2789 6565 8277 2789
Size: 3776 1712 2829
Fragments arranged by size:
3776 2829 1712
SexAI A'CCwGG_T
Cuts at: 3000 3000
Size: 8317
SfcI C'TryA_G
Cuts at: 826 1045 2797 3375 3696 4319 4460 4958 5002
Size: 219 1752 578 321 623 141 498 44
Cuts at: 5002 5060 5576 5665 6800 7481 7672 826
Size: 58 516 89 1135 681 191 1471
Fragments arranged by size:
1752 1471 1135 681 623 578 516 498
321 219 191 141 89 58 44
SfoI GGC'GCC
Cuts at: 2195 4615 2195
Size: 2420 5897
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8317
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8317
SmaI CCC'GGG
Cuts at: 1872 2089 4043 4109 4394 4694 5644 1872
Size: 217 1954 66 285 300 950 4545
Fragments arranged by size:
4545 1954 950 300 285 217 66
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2504 4142 4178 4826 5114 6421
Size: 197 926 561 1638 36 648 288 1307
Cuts at: 6421 7322 7584 820
Size: 901 262 1553
Fragments arranged by size:
1638 1553 1307 926 901 648 561 288
262 197 36
SnaBI TAC'GTA
Cuts at: 493 6108 493
Size: 5615 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8317
SphI G_CATG'C
Cuts at: 1132 4269 4628 5675 1132
Size: 3137 359 1047 3774
Fragments arranged by size:
3774 3137 1047 359
SrfI GCCC'GGGC
Cuts at: 2089 2089
Size: 8317
SspI AAT'ATT
Cuts at: 5 52 6241 5
Size: 47 6189 2081
Fragments arranged by size:
6189 2081 47
StuI AGG'CCT
Cuts at: 1517 2837 1517
Size: 1320 6997
StyI C'CwwG_G
Cuts at: 513 1065 2004 3168 3405 4191 5325 5756 513
Size: 552 939 1164 237 786 1134 431 3074
Fragments arranged by size:
3074 1164 1134 939 786 552 431 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2231 2272 3454 3752 4625 6344 6361
Size: 1255 651 41 1182 298 873 1719 17
Cuts at: 6361 6514 6699 7144 325
Size: 153 185 445 1498
Fragments arranged by size:
1719 1498 1255 1182 873 651 445 298
185 153 41 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2787 3543 5160
Size: 274 80 33 51 1443 810 756 1617
Cuts at: 5160 5274 6563 8275 96
Size: 114 1289 1712 138
Fragments arranged by size:
1712 1617 1443 1289 810 756 274 138
114 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8277 8309 8277
Size: 32 8285
Tth111I GACn'n_nGTC
Cuts at: 1316 2969 5570 1316
Size: 1653 2601 4063
Fragments arranged by size:
4063 2601 1653
XbaI T'CTAG_A
Cuts at: 3772 5653 3772
Size: 1881 6436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2467 3628 4852 2467
Size: 1161 1224 5932
Fragments arranged by size:
5932 1224 1161
XhoI C'TCGA_G
Cuts at: 1943 4826 1943
Size: 2883 5434
XmaI C'CCGG_G
Cuts at: 1870 2087 4041 4107 4392 4692 5642 1870
Size: 217 1954 66 285 300 950 4545
Fragments arranged by size:
4545 1954 950 300 285 217 66
XmnI GAAnn'nnTTC
Cuts at: 3827 6446 3827
Size: 2619 5698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6125 276
Size: 53 83 186 936 4591 2468
Fragments arranged by size:
4591 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SanDI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I
EcoRV FseI FspAI NruI NsiI PacI PsrI SacII
SapI SfiI SwaI