Restriction map for
pFN21AA1805
(Circular) (Six-base) MAPSORT of: pFN21AA1805.seq Check: 3539 from: 1 to: 6406
pFN21AA1805 6406 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4216 278
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3045 3252 3749 1937
Size: 1108 207 497 4594
Fragments arranged by size:
4594 1108 497 207
Acc65I G'GTAC_C
Cuts at: 3118 3727 3118
Size: 609 5797
AclI AA'CG_TT
Cuts at: 1563 4533 4906 1563
Size: 2970 373 3063
Fragments arranged by size:
3063 2970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3092 3151 4469 5853 6121 1632
Size: 1460 59 1318 1384 268 1917
Fragments arranged by size:
1917 1460 1384 1318 268 59
AflII C'TTAA_G
Cuts at: 820 1017 2158 820
Size: 197 1141 5068
Fragments arranged by size:
5068 1141 197
AflIII A'CryG_T
Cuts at: 1203 5305 6282 1203
Size: 4102 977 1327
Fragments arranged by size:
4102 1327 977
AgeI A'CCGG_T
Cuts at: 5237 5237
Size: 6406
AhdI GACnn_n'nnGTC
Cuts at: 5135 5135
Size: 6406
AleI CACnn'nnGTG
Cuts at: 1166 2877 1166
Size: 1711 4695
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6406
AlwNI CAG_nnn'CTG
Cuts at: 3061 3115 4149 5721 3061
Size: 54 1034 1572 3746
Fragments arranged by size:
3746 1572 1034 54
ApaLI G'TGCA_C
Cuts at: 4463 5619 4463
Size: 1156 5250
ApoI r'AATT_y
Cuts at: 1493 2348 3347 3715 3941 4093 6202 1493
Size: 855 999 368 226 152 2109 1697
Fragments arranged by size:
2109 1697 999 855 368 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4960 160
Size: 872 3928 1606
Fragments arranged by size:
3928 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
AvaI C'yCGr_G
Cuts at: 1870 1943 3548 3731 1870
Size: 73 1605 183 4545
Fragments arranged by size:
4545 1605 183 73
BamHI G'GATC_C
Cuts at: 3736 4103 3736
Size: 367 6039
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3118 3727 5182 618
Size: 325 217 627 1331 609 1455 1842
Fragments arranged by size:
1842 1455 1331 627 609 325 217
BanII G_rGCy'C
Cuts at: 1391 3159 3290 3725 1391
Size: 1768 131 435 4072
Fragments arranged by size:
4072 1768 435 131
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2001 2343 2549 3200 3323 928
Size: 971 102 342 206 651 123 4011
Fragments arranged by size:
4011 971 651 342 206 123 102
BbvCI CC'TCA_GC
Cuts at: 3396 3396
Size: 6406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2059 2093 4597 4631 1903
Size: 34 122 34 2504 34 3678
Fragments arranged by size:
3678 2504 122 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4298 5508 4298
Size: 1210 5196
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5017 136
Size: 107 122 71 631 605 3345 1525
Fragments arranged by size:
3345 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6401 6401
Size: 6406
BlpI GC'TnA_GC
Cuts at: 3823 3823
Size: 6406
Bme1580I G_kGCm'C
Cuts at: 1790 4467 5623 1790
Size: 2677 1156 2573
Fragments arranged by size:
2677 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6406
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2098 2756 4137
Size: 416 53 83 399 24 681 658 1381
Cuts at: 4137 5095 442
Size: 958 1753
Fragments arranged by size:
1753 1381 958 681 658 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6406
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2794 3082 2794
Size: 288 6118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2764 5066 1239
Size: 1525 2302 2579
Fragments arranged by size:
2579 2302 1525
Bpu10I CC'TnA_GC
Cuts at: 2756 3396 2756
Size: 640 5766
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4531 5396 5694 4531
Size: 865 298 5243
Fragments arranged by size:
5243 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3516 5069 882
Size: 617 2017 1553 2219
Fragments arranged by size:
2219 2017 1553 617
BsaAI yAC'GTr
Cuts at: 493 1204 4197 493
Size: 711 2993 2702
Fragments arranged by size:
2993 2702 711
BsaBI GATnn'nnATC
Cuts at: 6400 6400
Size: 6406
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4213 4595 5230 275
Size: 53 83 186 936 2680 382 635 1451
Fragments arranged by size:
2680 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4839 5237 5511 5658 6069 4839
Size: 398 274 147 411 5176
Fragments arranged by size:
5176 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2465 2495 3368 3398 1178
Size: 30 165 30 1062 30 873 30 4186
Fragments arranged by size:
4186 1062 873 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2080 2486 3601 1186
Size: 894 406 1115 3991
Fragments arranged by size:
3991 1115 894 406
BseYI C'CCAG_C
Cuts at: 5609 6105 5609
Size: 496 5910
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5833
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3856 4617 5645 1540
Size: 275 186 1855 761 1028 2301
Fragments arranged by size:
2301 1855 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2072 2111 4467 4552 5623 2072
Size: 39 2356 85 1071 2855
Fragments arranged by size:
2855 2356 1071 85 39
BsmI GAATG_Cn'
Cuts at: 3917 4010 3917
Size: 93 6313
BsmBI CGTCTCn'nnnn_
Cuts at: 3016 3016
Size: 6406
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2111 3159 3290 3725 4467 4552
Size: 399 282 39 1048 131 435 742 85
Cuts at: 4552 5623 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1048 742 435 399 282 131
85 39
BspEI T'CCGG_A
Cuts at: 6069 6069
Size: 6406
BspHI T'CATG_A
Cuts at: 2146 4295 6025 2146
Size: 2149 1730 2527
Fragments arranged by size:
2527 2149 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2531 3761 844
Size: 1687 1230 3489
Fragments arranged by size:
3489 1687 1230
BsrBI CCG'CTC
Cuts at: 1394 1823 4293 1394
Size: 429 2470 3507
Fragments arranged by size:
3507 2470 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2483 4901 5075 65
Size: 1183 1235 2418 174 1396
Fragments arranged by size:
2418 1396 1235 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5050 5237 1700
Size: 110 3240 187 2869
Fragments arranged by size:
3240 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3293 96
Size: 3197 3209
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6406
BssSI C'ACGA_G
Cuts at: 2071 4466 5478 2071
Size: 2395 1012 2999
Fragments arranged by size:
2999 2395 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6406
BstBI TT'CG_AA
Cuts at: 5210 5210
Size: 6406
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6406
BstXI CCAn_nnnn'nTGG
Cuts at: 2800 2800
Size: 6406
BstYI r'GATC_y
Cuts at: 1972 3736 4103 4488 4505 5957 6043 6055 6401
Size: 1764 367 385 17 1452 86 12 346
Cuts at: 6401 1972
Size: 1977
Fragments arranged by size:
1977 1764 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3046 3046
Size: 6406
Bsu36I CC'TnA_GG
Cuts at: 2921 2921
Size: 6406
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5854
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5667
Fragments arranged by size:
5667 683 56
BtsI GCAGTG_nn'
Cuts at: 2512 2538 3265 3922 4716 4736 2512
Size: 26 727 657 794 20 4182
Fragments arranged by size:
4182 794 727 657 26 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6406
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6371
DraI TTT'AAA
Cuts at: 2559 3710 2559
Size: 1151 5255
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5413 809
Size: 507 4097 1802
Fragments arranged by size:
4097 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3078 3853 4742
Size: 54 1425 50 275 18 1248 775 889
Cuts at: 4742 6371 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1248 889 775 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3853 1537
Size: 275 2041 4090
Fragments arranged by size:
4090 2041 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4336 1353
Size: 2983 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4993 5367 5513 387
Size: 1064 138 3404 374 146 1280
Fragments arranged by size:
3404 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2764 3092 3151 4469 5066 5853 6121
Size: 393 1132 328 59 1318 597 787 268
Cuts at: 6121 1239
Size: 1524
Fragments arranged by size:
1524 1318 1132 787 597 393 328 268
59
EcoO109I rG'GnC_Cy
Cuts at: 2042 2100 3112 2042
Size: 58 1012 5336
Fragments arranged by size:
5336 1012 58
EcoRI G'AATT_C
Cuts at: 3715 4093 3715
Size: 378 6028
EcoRV GAT'ATC
Cuts at: 2807 3202 2807
Size: 395 6011
FspI TGC'GCA
Cuts at: 4912 4912
Size: 6406
HaeII r_GCGC'y
Cuts at: 1678 5553 1678
Size: 3875 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2629 2661 5047 5079 5121
Size: 32 135 32 1057 32 2386 32 42
Cuts at: 5121 5153 1373
Size: 32 2626
Fragments arranged by size:
2626 2386 1057 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3750 3996 669
Size: 988 281 1812 246 3079
Fragments arranged by size:
3079 1812 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2369 3433 748
Size: 1621 1064 3721
Fragments arranged by size:
3721 1621 1064
HpaI GTT'AAC
Cuts at: 3996 3996
Size: 6406
KpnI G_GTAC'C
Cuts at: 3122 3731 3122
Size: 609 5797
MfeI C'AATT_G
Cuts at: 4005 4005
Size: 6406
MluI A'CGCG_T
Cuts at: 6282 6282
Size: 6406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5520 5704 1305
Size: 4215 184 2007
Fragments arranged by size:
4215 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3080 10
Size: 54 1425 343 1248 3336
Fragments arranged by size:
3336 1425 1248 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2182 2607 2877 4364 4723 4882 518
Size: 648 1016 425 270 1487 359 159 2042
Fragments arranged by size:
2042 1487 1016 648 425 359 270 159
MspA1I CmG'CkG
Cuts at: 1606 2261 3463 3822 4499 5647 5892 1606
Size: 655 1202 359 677 1148 245 2120
Fragments arranged by size:
2120 1202 1148 677 655 359 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5854
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6406
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6406
NotI GC'GGCC_GC
Cuts at: 3853 3853
Size: 6406
NspI r_CATG'y
Cuts at: 1132 1229 2767 3764 5309 1132
Size: 97 1538 997 1545 2229
Fragments arranged by size:
2229 1545 1538 997 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6406
PciI A'CATG_T
Cuts at: 5305 5305
Size: 6406
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2533 3087 1108
Size: 1425 554 4427
Fragments arranged by size:
4427 1425 554
PmeI GTTT'AAAC
Cuts at: 3710 3710
Size: 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3389 3421 4475 4507 5988 6020 3389
Size: 32 1054 32 1481 32 3775
Fragments arranged by size:
3775 1481 1054 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2042 2100 3112 2042
Size: 58 1012 5336
Fragments arranged by size:
5336 1012 58
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6406
PsiI TTA'TAA
Cuts at: 3976 3976
Size: 6406
PstI C_TGCA'G
Cuts at: 830 2845 3758 830
Size: 2015 913 3478
Fragments arranged by size:
3478 2015 913
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6406
PvuII CAG'CTG
Cuts at: 2261 2261
Size: 6406
SalI G'TCGA_C
Cuts at: 1936 3748 1936
Size: 1812 4594
SanDI GG'GwC_CC
Cuts at: 2042 2042
Size: 6406
SbfI CC_TGCA'GG
Cuts at: 3758 3758
Size: 6406
ScaI AGT'ACT
Cuts at: 3180 4654 6366 3180
Size: 1474 1712 3220
Fragments arranged by size:
3220 1712 1474
SexAI A'CCwGG_T
Cuts at: 3114 3114
Size: 6406
SfcI C'TryA_G
Cuts at: 826 1045 2841 3053 3253 3754 4889 5570 5761
Size: 219 1796 212 200 501 1135 681 191
Cuts at: 5761 826
Size: 1471
Fragments arranged by size:
1796 1471 1135 681 501 219 212 200
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6406
SmaI CCC'GGG
Cuts at: 1872 3733 1872
Size: 1861 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2158 4510 5411 5673 820
Size: 197 926 215 2352 901 262 1553
Fragments arranged by size:
2352 1553 926 901 262 215 197
SnaBI TAC'GTA
Cuts at: 493 4197 493
Size: 3704 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6406
SphI G_CATG'C
Cuts at: 1132 2767 3764 1132
Size: 1635 997 3774
Fragments arranged by size:
3774 1635 997
SspI AAT'ATT
Cuts at: 5 52 4330 5
Size: 47 4278 2081
Fragments arranged by size:
4278 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6406
StyI C'CwwG_G
Cuts at: 513 1065 2301 3081 3845 513
Size: 552 1236 780 764 3074
Fragments arranged by size:
3074 1236 780 764 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4433 4450 4603 4788 5233 325
Size: 1255 2853 17 153 185 445 1498
Fragments arranged by size:
2853 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2133 3178 3293
Size: 274 80 33 51 1443 156 1045 115
Cuts at: 3293 4652 6364 96
Size: 1359 1712 138
Fragments arranged by size:
1712 1443 1359 1045 274 156 138 115
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3294 3326 3368 3400 6366 6398 3294
Size: 32 42 32 2966 32 3302
Fragments arranged by size:
3302 2966 42 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6406
XbaI T'CTAG_A
Cuts at: 3742 3742
Size: 6406
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6406
XmaI C'CCGG_G
Cuts at: 1870 3731 1870
Size: 1861 4545
XmnI GAAnn'nnTTC
Cuts at: 4535 4535
Size: 6406
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4214 276
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI DraIII EcoICRI EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI
SfiI SfoI SrfI SwaI XcmI