Restriction map for
pFN21AA1803
(Circular) (Six-base) MAPSORT of: pFN21AA1803.seq Check: 7806 from: 1 to: 8941
pFN21AA1803 8941 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5654 6046 5654
Size: 392 8549
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6751 278
Size: 53 83 186 936 5215 2468
Fragments arranged by size:
5215 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2836 3081 6284 1937
Size: 899 245 3203 4594
Fragments arranged by size:
4594 3203 899 245
Acc65I G'GTAC_C
Cuts at: 6262 6262
Size: 8941
AclI AA'CG_TT
Cuts at: 1563 7068 7441 1563
Size: 5505 373 3063
Fragments arranged by size:
5505 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2901 3435 5036 5148 5304 5922 7004 8388
Size: 1269 534 1601 112 156 618 1082 1384
Cuts at: 8388 8656 1632
Size: 268 1917
Fragments arranged by size:
1917 1601 1384 1269 1082 618 534 268
156 112
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8744
AflIII A'CryG_T
Cuts at: 1203 6016 7840 8817 1203
Size: 4813 1824 977 1327
Fragments arranged by size:
4813 1824 1327 977
AgeI A'CCGG_T
Cuts at: 3270 7772 3270
Size: 4502 4439
AhdI GACnn_n'nnGTC
Cuts at: 7670 7670
Size: 8941
AleI CACnn'nnGTG
Cuts at: 1166 3416 1166
Size: 2250 6691
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4365 1184
Size: 3181 5760
AlwNI CAG_nnn'CTG
Cuts at: 2658 2816 4544 4738 6684 8256 2658
Size: 158 1728 194 1946 1572 3343
Fragments arranged by size:
3343 1946 1728 1572 194 158
ApaI G_GGCC'C
Cuts at: 2028 5474 2028
Size: 3446 5495
ApaLI G'TGCA_C
Cuts at: 2953 3185 4466 6998 8154 2953
Size: 232 1281 2532 1156 3740
Fragments arranged by size:
3740 2532 1281 1156 232
ApoI r'AATT_y
Cuts at: 1493 3350 4289 4451 5303 5312 5972 6250 6476
Size: 1857 939 162 852 9 660 278 226
Cuts at: 6476 6628 8737 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1857 1697 939 852 660 278 226
162 152 9
AseI AT'TA_AT
Cuts at: 160 1032 7495 160
Size: 872 6463 1606
Fragments arranged by size:
6463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8941
AvaI C'yCGr_G
Cuts at: 1870 1943 3395 3401 5462 6266 1870
Size: 73 1452 6 2061 804 4545
Fragments arranged by size:
4545 2061 1452 804 73 6
BamHI G'GATC_C
Cuts at: 6271 6638 6271
Size: 367 8574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3074 3263 3522 4408 4616
Size: 325 217 627 1287 189 259 886 208
Cuts at: 4616 6262 7717 618
Size: 1646 1455 1842
Fragments arranged by size:
1842 1646 1455 1287 886 627 325 259
217 208 189
BanII G_rGCy'C
Cuts at: 1391 2028 2511 3402 3425 3468 3604 5169 5474
Size: 637 483 891 23 43 136 1565 305
Cuts at: 5474 5855 6002 6260 1391
Size: 381 147 258 4072
Fragments arranged by size:
4072 1565 891 637 483 381 305 258
147 136 43 23
BbeI G_GCGC'C
Cuts at: 3267 4620 3267
Size: 1353 7588
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5145 5910 928
Size: 971 3246 765 3959
Fragments arranged by size:
3959 3246 971 765
BbvCI CC'TCA_GC
Cuts at: 3174 3801 3174
Size: 627 8314
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2229 2263 3689 3723 4470 4504 7132
Size: 34 292 34 1426 34 747 34 2628
Cuts at: 7132 7166 1903
Size: 34 3678
Fragments arranged by size:
3678 2628 1426 747 292 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4685 6833 8043 4685
Size: 2148 1210 5583
Fragments arranged by size:
5583 2148 1210
BclI T'GATC_A
Cuts at: 1548 3768 1548
Size: 2220 6721
BfrBI ATG'CAT
Cuts at: 4132 4132
Size: 8941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7552 136
Size: 107 122 71 631 605 5880 1525
Fragments arranged by size:
5880 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5479 8936 5479
Size: 3457 5484
BlpI GC'TnA_GC
Cuts at: 3806 6358 3806
Size: 2552 6389
Bme1580I G_kGCm'C
Cuts at: 1790 2028 2957 3189 3640 4470 5451 5474 7002
Size: 238 929 232 451 830 981 23 1528
Cuts at: 7002 8158 1790
Size: 1156 2573
Fragments arranged by size:
2573 1528 1156 981 929 830 451 238
232 23
BmgBI CAC'GTC
Cuts at: 1318 2427 3226 4510 1318
Size: 1109 799 1284 5749
Fragments arranged by size:
5749 1284 1109 799
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3361 6672 7630
Size: 416 53 83 399 24 1944 3311 958
Cuts at: 7630 442
Size: 1753
Fragments arranged by size:
3311 1944 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2915 1056
Size: 1859 7082
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4279 4279
Size: 8941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 3721 5635 5651 5839 6236 7601 1239
Size: 1085 1397 1914 16 188 397 1365 2579
Fragments arranged by size:
2579 1914 1397 1365 1085 397 188 16
Bpu10I CC'TnA_GC
Cuts at: 3174 3801 4053 3174
Size: 627 252 8062
Fragments arranged by size:
8062 627 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5015 7066 7931 8229 5015
Size: 2051 865 298 5727
Fragments arranged by size:
5727 2051 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3508 5723 7604 882
Size: 617 2009 2215 1881 2219
Fragments arranged by size:
2219 2215 2009 1881 617
BsaAI yAC'GTr
Cuts at: 493 1204 5437 6732 493
Size: 711 4233 1295 2702
Fragments arranged by size:
4233 2702 1295 711
BsaBI GATnn'nnATC
Cuts at: 8935 8935
Size: 8941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3264 4608 4617 5344
Size: 53 83 186 936 1731 1344 9 727
Cuts at: 5344 6748 7130 7765 275
Size: 1404 382 635 1451
Fragments arranged by size:
1731 1451 1404 1344 936 727 635 382
186 83 53 9
BsaWI w'CCGG_w
Cuts at: 2145 3270 3519 7374 7772 8046 8193 8604 2145
Size: 1125 249 3855 398 274 147 411 2482
Fragments arranged by size:
3855 2482 1125 411 398 274 249 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3455 3485 3488 3518 3530
Size: 30 165 30 2052 30 3 30 12
Cuts at: 3530 3560 5090 5120 1178
Size: 30 1530 30 4999
Fragments arranged by size:
4999 2052 1530 165 30 30 30 30
30 30 12 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3245 3461 3464 3476 4025 4333 4768 5072
Size: 2059 216 3 12 549 308 435 304
Cuts at: 5072 5077 5095 5308 5480 1186
Size: 5 18 213 172 4647
Fragments arranged by size:
4647 2059 549 435 308 304 216 213
172 18 12 5 3
BseYI C'CCAG_C
Cuts at: 2591 3603 4528 5444 8144 8640 2591
Size: 1012 925 916 2700 496 2892
Fragments arranged by size:
2892 2700 1012 925 916 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5531 1163
Size: 573 3795 4573
Fragments arranged by size:
4573 3795 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2254 3966 4685 6391 7152 8180
Size: 275 186 253 1712 719 1706 761 1028
Cuts at: 8180 1540
Size: 2301
Fragments arranged by size:
2301 1712 1706 1028 761 719 275 253
186
BsiHKAI G_wGCw'C
Cuts at: 2332 2351 2957 3189 3848 3857 4470 4988 5855
Size: 19 606 232 659 9 613 518 867
Cuts at: 5855 7002 7087 8158 2332
Size: 1147 85 1071 3115
Fragments arranged by size:
3115 1147 1071 867 659 613 606 518
232 85 19 9
BsmI GAATG_Cn'
Cuts at: 2708 6226 6452 6545 2708
Size: 3518 226 93 5104
Fragments arranged by size:
5104 3518 226 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3227 3227
Size: 8941
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2332 2351 2511 2957 3189 3402
Size: 399 238 304 19 160 446 232 213
Cuts at: 3402 3425 3468 3604 3640 3848 3857 4470 4988
Size: 23 43 136 36 208 9 613 518
Cuts at: 4988 5169 5451 5474 5855 6002 6260 7002 7087
Size: 181 282 23 381 147 258 742 85
Cuts at: 7087 8158 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 613 518 446 399 381
304 282 258 238 232 213 208 181
160 147 136 85 43 36 23 23
19 9
BspEI T'CCGG_A
Cuts at: 8604 8604
Size: 8941
BspHI T'CATG_A
Cuts at: 4747 6830 8560 4747
Size: 2083 1730 5128
Fragments arranged by size:
5128 2083 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5654 6046 6296 844
Size: 4810 392 250 3489
Fragments arranged by size:
4810 3489 392 250
BsrBI CCG'CTC
Cuts at: 1394 1823 2308 6828 1394
Size: 429 485 4520 3507
Fragments arranged by size:
4520 3507 485 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3094 4528 4696 7436 7610 65
Size: 1183 1846 1434 168 2740 174 1396
Fragments arranged by size:
2740 1846 1434 1396 1183 174 168
BsrFI r'CCGG_y
Cuts at: 1700 1810 3270 4923 5583 7585 7772 1700
Size: 110 1460 1653 660 2002 187 2869
Fragments arranged by size:
2869 2002 1653 1460 660 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8941
BssSI C'ACGA_G
Cuts at: 3550 7001 8013 3550
Size: 3451 1012 4478
Fragments arranged by size:
4478 3451 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2961 3096 4089 4358 4738 4896 5394 794
Size: 2167 135 993 269 380 158 498 4341
Fragments arranged by size:
4341 2167 993 498 380 269 158 135
BstBI TT'CG_AA
Cuts at: 2239 2667 7745 2239
Size: 428 5078 3435
Fragments arranged by size:
5078 3435 428
BstEII G'GTnAC_C
Cuts at: 1184 3166 1184
Size: 1982 6959
BstXI CCAn_nnnn'nTGG
Cuts at: 3946 4000 3946
Size: 54 8887
BstYI r'GATC_y
Cuts at: 1972 3243 5479 6271 6638 7023 7040 8492 8578
Size: 1271 2236 792 367 385 17 1452 86
Cuts at: 8578 8590 8936 1972
Size: 12 346 1977
Fragments arranged by size:
2236 1977 1452 1271 792 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2837 2837
Size: 8941
BtgI C'CryG_G
Cuts at: 513 1065 3121 4864 5038 5066 5932 513
Size: 552 2056 1743 174 28 866 3522
Fragments arranged by size:
3522 2056 1743 866 552 174 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2452 5877 487
Size: 683 56 1226 3425 3551
Fragments arranged by size:
3551 3425 1226 683 56
BtsI GCAGTG_nn'
Cuts at: 2351 2476 3721 3834 4110 4645 5296 6457 7251
Size: 125 1245 113 276 535 651 1161 794
Cuts at: 7251 7271 2351
Size: 20 4021
Fragments arranged by size:
4021 1245 1161 794 651 535 276 125
113 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5347 5382 529
Size: 35 4783 35 4088
Fragments arranged by size:
4783 4088 35 35
DraI TTT'AAA
Cuts at: 5416 6245 5416
Size: 829 8112
DraIII CAC_nnn'GTG
Cuts at: 3514 5542 3514
Size: 2028 6913
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7948 809
Size: 507 6632 1802
Fragments arranged by size:
6632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2042 2229 3963
Size: 54 1425 50 275 18 212 187 1734
Cuts at: 3963 4682 4770 6388 7277 8906 8
Size: 719 88 1618 889 1629 43
Fragments arranged by size:
1734 1629 1618 1425 889 719 275 212
187 88 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3963 4682 6388 1537
Size: 275 2151 719 1706 4090
Fragments arranged by size:
4090 2151 1706 719 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2796 3632 4002 4398 5550 6871 1353
Size: 1443 836 370 396 1152 1321 3423
Fragments arranged by size:
3423 1443 1321 1152 836 396 370
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4574 7528 7902 8048 387
Size: 1064 138 2985 2954 374 146 1280
Fragments arranged by size:
2985 2954 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5853 5853
Size: 8941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 2901 3435 3721 5036 5148 5304
Size: 393 692 577 534 286 1315 112 156
Cuts at: 5304 5635 5651 5839 5922 6236 7004 7601 8388
Size: 331 16 188 83 314 768 597 787
Cuts at: 8388 8656 1239
Size: 268 1524
Fragments arranged by size:
1524 1315 787 768 692 597 577 534
393 331 314 286 268 188 156 112
83 16
EcoO109I rG'GnC_Cy
Cuts at: 2024 2025 2813 4156 5470 5471 2024
Size: 1 788 1343 1314 1 5494
Fragments arranged by size:
5494 1343 1314 788 1 1
EcoRI G'AATT_C
Cuts at: 4289 5303 5312 6250 6628 4289
Size: 1014 9 938 378 6602
Fragments arranged by size:
6602 1014 938 378 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3865 4120 4186 5309 5656 3865
Size: 255 66 1123 347 7150
Fragments arranged by size:
7150 1123 347 255 66
FspI TGC'GCA
Cuts at: 7447 7447
Size: 8941
HaeII r_GCGC'y
Cuts at: 1678 3267 3697 4620 8088 1678
Size: 1589 430 923 3468 2531
Fragments arranged by size:
3468 2531 1589 923 430
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3455 3487 4878 4910 5062
Size: 32 135 32 1883 32 1391 32 152
Cuts at: 5062 5094 6057 6089 7582 7614 7656 7688 1373
Size: 32 963 32 1493 32 42 32 2626
Fragments arranged by size:
2626 1883 1493 1391 963 152 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5812 6285 6531 669
Size: 988 281 3874 473 246 3079
Fragments arranged by size:
3874 3079 988 473 281 246
HindIII A'AGCT_T
Cuts at: 748 5375 748
Size: 4627 4314
HpaI GTT'AAC
Cuts at: 5812 6531 5812
Size: 719 8222
KasI G'GCGC_C
Cuts at: 3263 4616 3263
Size: 1353 7588
KpnI G_GTAC'C
Cuts at: 6266 6266
Size: 8941
MfeI C'AATT_G
Cuts at: 6540 6540
Size: 8941
MluI A'CGCG_T
Cuts at: 8817 8817
Size: 8941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4647 5782 8055 8239 1305
Size: 3342 1135 2273 184 2007
Fragments arranged by size:
3342 2273 2007 1135 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4772 10
Size: 54 1425 343 2940 4179
Fragments arranged by size:
4179 2940 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2335 2570 2950 3029 3164 3416 3591
Size: 648 1169 235 380 79 135 252 175
Cuts at: 3591 3998 4885 4956 6899 7258 7417 518
Size: 407 887 71 1943 359 159 2042
Fragments arranged by size:
2042 1943 1169 887 648 407 380 359
252 235 175 159 135 79 71
MspA1I CmG'CkG
Cuts at: 1606 2065 2277 3173 3805 4080 4532 4541 4800
Size: 459 212 896 632 275 452 9 259
Cuts at: 4800 5014 5207 5625 5824 6357 7034 8182 8427
Size: 214 193 418 199 533 677 1148 245
Cuts at: 8427 1606
Size: 2120
Fragments arranged by size:
2120 1148 896 677 632 533 459 452
418 275 259 245 214 212 199 193
9
NaeI GCC'GGC
Cuts at: 4925 4925
Size: 8941
NarI GG'CG_CC
Cuts at: 3264 4617 3264
Size: 1353 7588
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8389
NdeI CA'TA_TG
Cuts at: 387 4851 387
Size: 4464 4477
NgoMIV G'CCGG_C
Cuts at: 4923 4923
Size: 8941
NheI G'CTAG_C
Cuts at: 1052 2911 1052
Size: 1859 7082
NotI GC'GGCC_GC
Cuts at: 6388 6388
Size: 8941
NsiI A_TGCA'T
Cuts at: 4134 4134
Size: 8941
NspI r_CATG'y
Cuts at: 1132 1229 4132 4967 6020 6299 7844 1132
Size: 97 2903 835 1053 279 1545 2229
Fragments arranged by size:
2903 2229 1545 1053 835 279 97
PasI CC'CwG_GG
Cuts at: 1348 2718 3911 1348
Size: 1370 1193 6378
Fragments arranged by size:
6378 1370 1193
PciI A'CATG_T
Cuts at: 6016 7840 6016
Size: 1824 7117
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8941
PfoI T'CCnGG_A
Cuts at: 2850 3737 4581 6213 2850
Size: 887 844 1632 5578
Fragments arranged by size:
5578 1632 887 844
PmeI GTTT'AAAC
Cuts at: 6245 6245
Size: 8941
PmlI CAC'GTG
Cuts at: 5437 5437
Size: 8941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5013 5045 7010 7042 8523 8555 5013
Size: 32 1965 32 1481 32 5399
Fragments arranged by size:
5399 1965 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4156 4156
Size: 8941
PshAI GACnn'nnGTC
Cuts at: 1613 3154 1613
Size: 1541 7400
PsiI TTA'TAA
Cuts at: 6511 6511
Size: 8941
PspOMI G'GGCC_C
Cuts at: 2024 5470 2024
Size: 3446 5495
PspXI vC'TCGA_Gb
Cuts at: 3395 3395
Size: 8941
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4196 4228 4196
Size: 32 8909
PstI C_TGCA'G
Cuts at: 830 2910 4117 6293 830
Size: 2080 1207 2176 3478
Fragments arranged by size:
3478 2176 2080 1207
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8941
PvuII CAG'CTG
Cuts at: 2065 2277 3805 4532 4541 4800 5014 5207 5824
Size: 212 1528 727 9 259 214 193 617
Cuts at: 5824 2065
Size: 5182
Fragments arranged by size:
5182 1528 727 617 259 214 212 193
9
SacI G_AGCT'C
Cuts at: 5855 5855
Size: 8941
SalI G'TCGA_C
Cuts at: 1936 6283 1936
Size: 4347 4594
SapI GCTCTTCn'nnn_
Cuts at: 5550 5550
Size: 8941
SbfI CC_TGCA'GG
Cuts at: 6293 6293
Size: 8941
ScaI AGT'ACT
Cuts at: 3539 7189 8901 3539
Size: 3650 1712 3579
Fragments arranged by size:
3650 3579 1712
SexAI A'CCwGG_T
Cuts at: 3498 3498
Size: 8941
SfcI C'TryA_G
Cuts at: 826 1045 2644 2906 4113 6289 7424 8105 8296
Size: 219 1599 262 1207 2176 1135 681 191
Cuts at: 8296 826
Size: 1471
Fragments arranged by size:
2176 1599 1471 1207 1135 681 262 219
191
SfoI GGC'GCC
Cuts at: 3265 4618 3265
Size: 1353 7588
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8941
SmaI CCC'GGG
Cuts at: 1872 6268 1872
Size: 4396 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3395 5030 7045 7946 8208 820
Size: 197 926 1452 1635 2015 901 262 1553
Fragments arranged by size:
2015 1635 1553 1452 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6732 493
Size: 6239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8941
SphI G_CATG'C
Cuts at: 1132 4132 4967 6299 1132
Size: 3000 835 1332 3774
Fragments arranged by size:
3774 3000 1332 835
SspI AAT'ATT
Cuts at: 5 52 6865 5
Size: 47 6813 2081
Fragments arranged by size:
6813 2081 47
StuI AGG'CCT
Cuts at: 1517 4387 1517
Size: 2870 6071
StyI C'CwwG_G
Cuts at: 513 1065 2029 3786 6380 513
Size: 552 964 1757 2594 3074
Fragments arranged by size:
3074 2594 1757 964 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2268 3924 4485 6093 6968 6985 7138
Size: 1255 688 1656 561 1608 875 17 153
Cuts at: 7138 7323 7768 325
Size: 185 445 1498
Fragments arranged by size:
1656 1608 1498 1255 875 688 561 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3537 4794 7187
Size: 274 80 33 51 1443 1560 1257 2393
Cuts at: 7187 8899 96
Size: 1712 138
Fragments arranged by size:
2393 1712 1560 1443 1257 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3488 3520 4049 4081 5090 5122 8901 8933 3488
Size: 32 529 32 1009 32 3779 32 3496
Fragments arranged by size:
3779 3496 1009 529 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4715 1316
Size: 3399 5542
XbaI T'CTAG_A
Cuts at: 2449 6277 2449
Size: 3828 5113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4618 4618
Size: 8941
XhoI C'TCGA_G
Cuts at: 1943 3395 1943
Size: 1452 7489
XmaI C'CCGG_G
Cuts at: 1870 6266 1870
Size: 4396 4545
XmnI GAAnn'nnTTC
Cuts at: 5378 7070 5378
Size: 1692 7249
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6749 276
Size: 53 83 186 936 5215 2468
Fragments arranged by size:
5215 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BsiWI Bsu36I EcoNI
EcoRV FseI FspAI NruI PacI RsrII SacII SanDI
SfiI SrfI SwaI