Restriction map for
pFN21AA1793
(Circular) (Six-base) MAPSORT of: pFN21AA1793.seq Check: 9090 from: 1 to: 8167
pFN21AA1793 8167 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4060 4490 4060
Size: 430 7737
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2840 5462 5977 278
Size: 53 83 186 936 1304 2622 515 2468
Fragments arranged by size:
2622 2468 1304 936 515 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5510 1937
Size: 3573 4594
Acc65I G'GTAC_C
Cuts at: 5488 5488
Size: 8167
AclI AA'CG_TT
Cuts at: 1563 6294 6667 1563
Size: 4731 373 3063
Fragments arranged by size:
4731 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2841 2943 3125 3173 3431 4554 4697 6230
Size: 1209 102 182 48 258 1123 143 1533
Cuts at: 6230 7614 7882 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1533 1384 1209 1123 268 258 182
143 102 48
AfeI AGC'GCT
Cuts at: 4547 4547
Size: 8167
AflII C'TTAA_G
Cuts at: 820 1017 2411 820
Size: 197 1394 6576
Fragments arranged by size:
6576 1394 197
AflIII A'CryG_T
Cuts at: 1203 3302 3504 7066 8043 1203
Size: 2099 202 3562 977 1327
Fragments arranged by size:
3562 2099 1327 977 202
AgeI A'CCGG_T
Cuts at: 6998 6998
Size: 8167
AhdI GACnn_n'nnGTC
Cuts at: 6896 6896
Size: 8167
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8167
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8167
AlwNI CAG_nnn'CTG
Cuts at: 2200 2461 2689 3117 3277 3340 4066 4506 5210
Size: 261 228 428 160 63 726 440 704
Cuts at: 5210 5910 7482 2200
Size: 700 1572 2885
Fragments arranged by size:
2885 1572 726 704 700 440 428 261
228 160 63
ApaI G_GGCC'C
Cuts at: 2731 2731
Size: 8167
ApaLI G'TGCA_C
Cuts at: 6224 7380 6224
Size: 1156 7011
ApoI r'AATT_y
Cuts at: 1493 5476 5702 5854 7963 1493
Size: 3983 226 152 2109 1697
Fragments arranged by size:
3983 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6721 160
Size: 872 5689 1606
Fragments arranged by size:
5689 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8167
AvaI C'yCGr_G
Cuts at: 1870 1943 2229 4938 5492 1870
Size: 73 286 2709 554 4545
Fragments arranged by size:
4545 2709 554 286 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4284 4317 4284
Size: 33 8134
BamHI G'GATC_C
Cuts at: 5497 5864 5497
Size: 367 7800
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5488 6943 618
Size: 325 217 627 3701 1455 1842
Fragments arranged by size:
3701 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2194 2280 2627 2731 3039 3168 3201 3561
Size: 803 86 347 104 308 129 33 360
Cuts at: 3561 3705 4107 4896 5486 1391
Size: 144 402 789 590 4072
Fragments arranged by size:
4072 803 789 590 402 360 347 308
144 129 104 86 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2559 3837 4542 5286 928
Size: 971 660 1278 705 744 3809
Fragments arranged by size:
3809 1278 971 744 705 660
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4505 4539 6358 6392 1903
Size: 34 50 34 2484 34 1819 34 3678
Fragments arranged by size:
3678 2484 1819 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3382 5011 6059 7269 3382
Size: 1629 1048 1210 4280
Fragments arranged by size:
4280 1629 1210 1048
BclI T'GATC_A
Cuts at: 1548 4575 1548
Size: 3027 5140
BfrBI ATG'CAT
Cuts at: 2935 2935
Size: 8167
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2312 4091 4397
Size: 107 122 71 631 605 640 1779 306
Cuts at: 4397 6778 136
Size: 2381 1525
Fragments arranged by size:
2381 1779 1525 640 631 605 306 122
107 71
BglII A'GATC_T
Cuts at: 2770 8162 2770
Size: 5392 2775
BlpI GC'TnA_GC
Cuts at: 3441 4527 5196 5584 3441
Size: 1086 669 388 6024
Fragments arranged by size:
6024 1086 669 388
Bme1580I G_kGCm'C
Cuts at: 1790 2731 6228 7384 1790
Size: 941 3497 1156 2573
Fragments arranged by size:
3497 2573 1156 941
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8167
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5898 6856 442
Size: 416 53 83 399 24 4481 958 1753
Fragments arranged by size:
4481 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2300 4821 1056
Size: 1244 2521 4402
Fragments arranged by size:
4402 2521 1244
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2275 2611 2275
Size: 336 7831
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2240 2303 2795 3269 3509 3539 3566
Size: 959 42 63 492 474 240 30 27
Cuts at: 3566 3728 4883 5075 6827 1239
Size: 162 1155 192 1752 2579
Fragments arranged by size:
2579 1752 1155 959 492 474 240 192
162 63 42 30 27
Bpu10I CC'TnA_GC
Cuts at: 2193 4904 2193
Size: 2711 5456
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3023 3545 3785 4247 4349 5057 6292 7157 7455
Size: 522 240 462 102 708 1235 865 298
Cuts at: 7455 3023
Size: 3735
Fragments arranged by size:
3735 1235 865 708 522 462 298 240
102
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2219 2654 5033 6830 882
Size: 617 720 435 2379 1797 2219
Fragments arranged by size:
2379 2219 1797 720 617 435
BsaAI yAC'GTr
Cuts at: 493 1204 5958 493
Size: 711 4754 2702
Fragments arranged by size:
4754 2702 711
BsaBI GATnn'nnATC
Cuts at: 2588 8161 2588
Size: 5573 2594
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2837 4083 5304 5459
Size: 53 83 186 936 1304 1246 1221 155
Cuts at: 5459 5974 6356 6991 275
Size: 515 382 635 1451
Fragments arranged by size:
1451 1304 1246 1221 936 635 515 382
186 155 83 53
BsaWI w'CCGG_w
Cuts at: 2636 3580 6600 6998 7272 7419 7830 2636
Size: 944 3020 398 274 147 411 2973
Fragments arranged by size:
3020 2973 944 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2262 2292 3026 3056 3092
Size: 30 165 30 859 30 734 30 36
Cuts at: 3092 3122 3245 3275 3341 3371 1178
Size: 30 123 30 66 30 5974
Fragments arranged by size:
5974 859 734 165 123 66 36 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2084 2195 2237 2387 3023 3026 3113
Size: 877 21 111 42 150 636 3 87
Cuts at: 3113 3362 3733 3746 4151 4904 5126 5141 5150
Size: 249 371 13 405 753 222 15 9
Cuts at: 5150 5282 5306 5309 1186
Size: 132 24 3 4044
Fragments arranged by size:
4044 877 753 636 405 371 249 222
150 132 111 87 42 24 21 15
13 9 3 3
BseYI C'CCAG_C
Cuts at: 2035 2307 4262 7370 7866 2035
Size: 272 1955 3108 496 2336
Fragments arranged by size:
3108 2336 1955 496 272
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2126 2625 3263 3743 4402 4492 4613
Size: 573 390 499 638 480 659 90 121
Cuts at: 4613 4871 1163
Size: 258 4459
Fragments arranged by size:
4459 659 638 573 499 480 390 258
121 90
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5617 6378 7406 1540
Size: 275 186 3616 761 1028 2301
Fragments arranged by size:
3616 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2627 3039 3049 3168 3201 3561 3705 4107 4896
Size: 412 10 119 33 360 144 402 789
Cuts at: 4896 6228 6313 7384 2627
Size: 1332 85 1071 3410
Fragments arranged by size:
3410 1332 1071 789 412 402 360 144
119 85 33 10
BsmI GAATG_Cn'
Cuts at: 2565 4730 4954 4978 5678 5771 2565
Size: 2165 224 24 700 93 4961
Fragments arranged by size:
4961 2165 700 224 93 24
BsmBI CGTCTCn'nnnn_
Cuts at: 3425 5450 3425
Size: 2025 6142
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2194 2280 2627 2731 3039 3049 3168
Size: 399 404 86 347 104 308 10 119
Cuts at: 3168 3201 3561 3705 4107 4896 5486 6228 6313
Size: 33 360 144 402 789 590 742 85
Cuts at: 6313 7384 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 789 742 590 404 402 399
360 347 308 144 119 104 86 85
33 10
BspEI T'CCGG_A
Cuts at: 3580 7830 3580
Size: 4250 3917
BspHI T'CATG_A
Cuts at: 3216 6056 7786 3216
Size: 2840 1730 3597
Fragments arranged by size:
3597 2840 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4060 4490 4531 5522 844
Size: 3216 430 41 991 3489
Fragments arranged by size:
3489 3216 991 430 41
BsrBI CCG'CTC
Cuts at: 1394 1823 3493 4141 6054 1394
Size: 429 1670 648 1913 3507
Fragments arranged by size:
3507 1913 1670 648 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3421 4273 6662 6836 65
Size: 1183 2173 852 2389 174 1396
Fragments arranged by size:
2389 2173 1396 1183 852 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3264 4085 6811 6998 1700
Size: 110 1454 821 2726 187 2869
Fragments arranged by size:
2869 2726 1454 821 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8167
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8167
BssSI C'ACGA_G
Cuts at: 6227 7239 6227
Size: 1012 7155
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2689 3277 3340 4066 4591 4725 794
Size: 1895 588 63 726 525 134 4236
Fragments arranged by size:
4236 1895 726 588 525 134 63
BstBI TT'CG_AA
Cuts at: 6971 6971
Size: 8167
BstEII G'GTnAC_C
Cuts at: 1184 4999 1184
Size: 3815 4352
BstYI r'GATC_y
Cuts at: 1972 2334 2451 2524 2770 3927 5206 5497 5864
Size: 362 117 73 246 1157 1279 291 367
Cuts at: 5864 6249 6266 7718 7804 7816 8162 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1279 1157 385 367 362 346
291 246 117 86 73 17 12
Bsu36I CC'TnA_GG
Cuts at: 3383 5211 3383
Size: 1828 6339
BtgI C'CryG_G
Cuts at: 513 1065 4740 513
Size: 552 3675 3940
Fragments arranged by size:
3940 3675 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2978 487
Size: 683 56 1752 5676
Fragments arranged by size:
5676 1752 683 56
BtsI GCAGTG_nn'
Cuts at: 2125 3362 3617 3701 3770 3776 4883 5180 5683
Size: 1237 255 84 69 6 1107 297 503
Cuts at: 5683 6477 6497 2125
Size: 794 20 3795
Fragments arranged by size:
3795 1237 1107 794 503 297 255 84
69 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8167
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8132
DraI TTT'AAA
Cuts at: 5471 5471
Size: 8167
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7174 809
Size: 507 5858 1802
Fragments arranged by size:
5858 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4659 5614 6503
Size: 54 1425 50 275 18 2829 955 889
Cuts at: 6503 8132 8
Size: 1629 43
Fragments arranged by size:
2829 1629 1425 955 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5614 1537
Size: 275 3802 4090
Fragments arranged by size:
4090 3802 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2049 2055 2061 2073 2615 2919 2973 3747
Size: 696 6 6 12 542 304 54 774
Cuts at: 3747 3930 4139 4235 4403 4445 4484 4677 4907
Size: 183 209 96 168 42 39 193 230
Cuts at: 4907 5244 5259 5283 5317 6097 1353
Size: 337 15 24 34 780 3423
Fragments arranged by size:
3423 780 774 696 542 337 304 230
209 193 183 168 96 54 42 39
34 24 15 12 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4886 6754 7128 7274 387
Size: 1064 138 3297 1868 374 146 1280
Fragments arranged by size:
3297 1868 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2625 3037 3166 3199 3559 3703 4105 4894 2625
Size: 412 129 33 360 144 402 789 5898
Fragments arranged by size:
5898 789 412 402 360 144 129 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2240 2303 2795 2841 2943 3125
Size: 393 566 42 63 492 46 102 182
Cuts at: 3125 3173 3269 3431 3509 3539 3566 3728 4554
Size: 48 96 162 78 30 27 162 826
Cuts at: 4554 4697 4883 5075 6230 6827 7614 7882 1239
Size: 143 186 192 1155 597 787 268 1524
Fragments arranged by size:
1524 1155 826 787 597 566 492 393
268 192 186 182 162 162 143 102
96 78 63 48 46 42 30 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2519 2714 4699 5409 2519
Size: 195 1985 710 5277
Fragments arranged by size:
5277 1985 710 195
EcoO109I rG'GnC_Cy
Cuts at: 2728 3526 4063 2728
Size: 798 537 6832
Fragments arranged by size:
6832 798 537
EcoRI G'AATT_C
Cuts at: 5476 5854 5476
Size: 378 7789
FspI TGC'GCA
Cuts at: 6673 6673
Size: 8167
HaeII r_GCGC'y
Cuts at: 1678 2307 4549 7314 1678
Size: 629 2242 2765 2531
Fragments arranged by size:
2765 2531 2242 629
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2145 2177 2443 2475 2495
Size: 32 135 32 573 32 266 32 20
Cuts at: 2495 2527 3092 3124 3424 3456 5294 5326 5447
Size: 32 565 32 300 32 1838 32 121
Cuts at: 5447 5479 6808 6840 6882 6914 1373
Size: 32 1329 32 42 32 2626
Fragments arranged by size:
2626 1838 1329 573 565 300 266 135
121 42 32 32 32 32 32 32
32 32 32 32 32 20
HincII GTy'rAC
Cuts at: 669 1657 1938 2140 5511 5757 669
Size: 988 281 202 3371 246 3079
Fragments arranged by size:
3371 3079 988 281 246 202
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8167
HpaI GTT'AAC
Cuts at: 5757 5757
Size: 8167
KpnI G_GTAC'C
Cuts at: 5492 5492
Size: 8167
MfeI C'AATT_G
Cuts at: 5766 5766
Size: 8167
MluI A'CGCG_T
Cuts at: 8043 8043
Size: 8167
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2955 3957 7281 7465 1305
Size: 1650 1002 3324 184 2007
Fragments arranged by size:
3324 2007 1650 1002 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4661 10
Size: 54 1425 343 2829 3516
Fragments arranged by size:
3516 2829 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2253 2499 3507 6125 6484 6643 518
Size: 648 1087 246 1008 2618 359 159 2042
Fragments arranged by size:
2618 2042 1087 1008 648 359 246 159
MspA1I CmG'CkG
Cuts at: 1606 2206 3181 3340 3463 3517 3544 3682 3745
Size: 600 975 159 123 54 27 138 63
Cuts at: 3745 3850 4189 4506 4633 4804 4861 4873 5583
Size: 105 339 317 127 171 57 12 710
Cuts at: 5583 6260 7408 7653 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 975 710 677 600 339 317
245 171 159 138 127 123 105 63
57 54 27 12
NaeI GCC'GGC
Cuts at: 3266 3266
Size: 8167
NcoI C'CATG_G
Cuts at: 513 1065 4740 513
Size: 552 3675 3940
Fragments arranged by size:
3940 3675 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8167
NgoMIV G'CCGG_C
Cuts at: 3264 3264
Size: 8167
NheI G'CTAG_C
Cuts at: 1052 2296 4817 1052
Size: 1244 2521 4402
Fragments arranged by size:
4402 2521 1244
NotI GC'GGCC_GC
Cuts at: 5614 5614
Size: 8167
NsiI A_TGCA'T
Cuts at: 2937 2937
Size: 8167
NspI r_CATG'y
Cuts at: 1132 1229 3306 3508 3868 5525 7070 1132
Size: 97 2077 202 360 1657 1545 2229
Fragments arranged by size:
2229 2077 1657 1545 360 202 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8167
PciI A'CATG_T
Cuts at: 3302 3504 7066 3302
Size: 202 3562 4403
Fragments arranged by size:
4403 3562 202
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3788 1108
Size: 2680 5487
PfoI T'CCnGG_A
Cuts at: 2280 3705 3966 2280
Size: 1425 261 6481
Fragments arranged by size:
6481 1425 261
PmeI GTTT'AAAC
Cuts at: 5471 5471
Size: 8167
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6236 6268 7749 7781 6236
Size: 32 1481 32 6622
Fragments arranged by size:
6622 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3526 3526
Size: 8167
PshAI GACnn'nnGTC
Cuts at: 1613 4086 1613
Size: 2473 5694
PsiI TTA'TAA
Cuts at: 5737 5737
Size: 8167
PspOMI G'GGCC_C
Cuts at: 2727 2727
Size: 8167
PstI C_TGCA'G
Cuts at: 830 2205 2520 2685 2736 2817 3030 3129 3273
Size: 1375 315 165 51 81 213 99 144
Cuts at: 3273 3345 3357 3378 3687 3750 3849 4071 4155
Size: 72 12 21 309 63 99 222 84
Cuts at: 4155 4587 4782 4803 4878 5519 830
Size: 432 195 21 75 641 3478
Fragments arranged by size:
3478 1375 641 432 315 309 222 213
195 165 144 99 99 84 81 75
72 63 51 21 21 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8167
PvuII CAG'CTG
Cuts at: 2206 3181 3340 3463 3517 3544 3682 3745 3850
Size: 975 159 123 54 27 138 63 105
Cuts at: 3850 4189 4506 4633 4804 4861 4873 2206
Size: 339 317 127 171 57 12 5500
Fragments arranged by size:
5500 975 339 317 171 159 138 127
123 105 63 57 54 27 12
SacI G_AGCT'C
Cuts at: 2627 3039 3168 3201 3561 3705 4107 4896 2627
Size: 412 129 33 360 144 402 789 5898
Fragments arranged by size:
5898 789 412 402 360 144 129 33
SalI G'TCGA_C
Cuts at: 1936 5509 1936
Size: 3573 4594
SapI GCTCTTCn'nnn_
Cuts at: 2615 2919 2973 3747 3930 4235 4677 2615
Size: 304 54 774 183 305 442 6105
Fragments arranged by size:
6105 774 442 305 304 183 54
SbfI CC_TGCA'GG
Cuts at: 2520 2736 4071 5519 2520
Size: 216 1335 1448 5168
Fragments arranged by size:
5168 1448 1335 216
ScaI AGT'ACT
Cuts at: 6415 8127 6415
Size: 1712 6455
SfcI C'TryA_G
Cuts at: 826 1045 2201 2270 2516 2681 2732 2813 2984
Size: 219 1156 69 246 165 51 81 171
Cuts at: 2984 3026 3125 3269 3341 3353 3374 3683 3746
Size: 42 99 144 72 12 21 309 63
Cuts at: 3746 3845 4067 4151 4583 4778 4799 4874 5515
Size: 99 222 84 432 195 21 75 641
Cuts at: 5515 6650 7331 7522 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1156 1135 681 641 432 309 246
222 219 195 191 171 165 144 99
99 84 81 75 72 69 63 51
42 21 21 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8167
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8167
SmaI CCC'GGG
Cuts at: 1872 2231 4940 5494 1872
Size: 359 2709 554 4545
Fragments arranged by size:
4545 2709 554 359
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2411 3038 3560 3800 4226 4364
Size: 197 926 468 627 522 240 426 138
Cuts at: 4364 5036 6271 7172 7434 820
Size: 672 1235 901 262 1553
Fragments arranged by size:
1553 1235 926 901 672 627 522 468
426 262 240 197 138
SnaBI TAC'GTA
Cuts at: 493 5958 493
Size: 5465 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8167
SphI G_CATG'C
Cuts at: 1132 3868 5525 1132
Size: 2736 1657 3774
Fragments arranged by size:
3774 2736 1657
SspI AAT'ATT
Cuts at: 5 52 6091 5
Size: 47 6039 2081
Fragments arranged by size:
6039 2081 47
StuI AGG'CCT
Cuts at: 1517 3566 4400 4918 5415 1517
Size: 2049 834 518 497 4269
Fragments arranged by size:
4269 2049 834 518 497
StyI C'CwwG_G
Cuts at: 513 1065 2262 2347 3859 4740 5606 513
Size: 552 1197 85 1512 881 866 3074
Fragments arranged by size:
3074 1512 1197 881 866 552 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3104 4355 6194 6211 6364 6549 6994
Size: 1255 1524 1251 1839 17 153 185 445
Cuts at: 6994 325
Size: 1498
Fragments arranged by size:
1839 1524 1498 1255 1251 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3651 5067 6413
Size: 274 80 33 51 1443 1674 1416 1346
Cuts at: 6413 8125 96
Size: 1712 138
Fragments arranged by size:
1712 1674 1443 1416 1346 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3026 3058 3245 3277 3341 3373 8127 8159 3026
Size: 32 187 32 64 32 4754 32 3034
Fragments arranged by size:
4754 3034 187 64 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5461 1316
Size: 4145 4022
XbaI T'CTAG_A
Cuts at: 5503 5503
Size: 8167
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 4045 5050 2011
Size: 2034 1005 5128
Fragments arranged by size:
5128 2034 1005
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8167
XmaI C'CCGG_G
Cuts at: 1870 2229 4938 5492 1870
Size: 359 2709 554 4545
Fragments arranged by size:
4545 2709 554 359
XmnI GAAnn'nnTTC
Cuts at: 2565 6296 2565
Size: 3731 4436
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2838 5460 5975 276
Size: 53 83 186 936 1304 2622 515 2468
Fragments arranged by size:
2622 2468 1304 936 515 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BbvCI BsiWI BstXI BstZ17I
DraIII EcoRV FalI FseI FspAI KasI NarI NruI
PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI