Restriction map for
pFN21AA1780
(Circular) (Six-base) MAPSORT of: pFN21AA1780.seq Check: 6570 from: 1 to: 8125
pFN21AA1780 8125 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2618 4126 4439 2618
Size: 1508 313 6304
Fragments arranged by size:
6304 1508 313
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5935 278
Size: 53 83 186 936 4399 2468
Fragments arranged by size:
4399 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5468 1937
Size: 3531 4594
Acc65I G'GTAC_C
Cuts at: 5446 5446
Size: 8125
AclI AA'CG_TT
Cuts at: 1563 6252 6625 1563
Size: 4689 373 3063
Fragments arranged by size:
4689 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4359 5193 6188 7572 7840 1632
Size: 2727 834 995 1384 268 1917
Fragments arranged by size:
2727 1917 1384 995 834 268
AfeI AGC'GCT
Cuts at: 3050 3626 3050
Size: 576 7549
AflII C'TTAA_G
Cuts at: 820 1017 4528 820
Size: 197 3511 4417
Fragments arranged by size:
4417 3511 197
AflIII A'CryG_T
Cuts at: 1203 2867 3200 3548 4146 7024 8001 1203
Size: 1664 333 348 598 2878 977 1327
Fragments arranged by size:
2878 1664 1327 977 598 348 333
AgeI A'CCGG_T
Cuts at: 6956 6956
Size: 8125
AhdI GACnn_n'nnGTC
Cuts at: 6854 6854
Size: 8125
AleI CACnn'nnGTG
Cuts at: 1166 3064 1166
Size: 1898 6227
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3667 4294 1184
Size: 2483 627 5015
Fragments arranged by size:
5015 2483 627
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3343 3375 3343
Size: 32 8093
AlwNI CAG_nnn'CTG
Cuts at: 2132 2418 4098 5868 7440 2132
Size: 286 1680 1770 1572 2817
Fragments arranged by size:
2817 1770 1680 1572 286
ApaI G_GGCC'C
Cuts at: 3740 3915 4133 3740
Size: 175 218 7732
Fragments arranged by size:
7732 218 175
ApaLI G'TGCA_C
Cuts at: 3286 3552 3810 4269 6182 7338 3286
Size: 266 258 459 1913 1156 4073
Fragments arranged by size:
4073 1913 1156 459 266 258
ApoI r'AATT_y
Cuts at: 1493 2166 4017 5041 5434 5660 5812 7921 1493
Size: 673 1851 1024 393 226 152 2109 1697
Fragments arranged by size:
2109 1851 1697 1024 673 393 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6679 160
Size: 872 5647 1606
Fragments arranged by size:
5647 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8125
AvaI C'yCGr_G
Cuts at: 1870 1943 2810 5450 1870
Size: 73 867 2640 4545
Fragments arranged by size:
4545 2640 867 73
BamHI G'GATC_C
Cuts at: 5293 5455 5822 5293
Size: 162 367 7596
Fragments arranged by size:
7596 367 162
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2457 2558 2922 3827 3962
Size: 325 217 627 670 101 364 905 135
Cuts at: 3962 5446 6901 618
Size: 1484 1455 1842
Fragments arranged by size:
1842 1484 1455 905 670 627 364 325
217 135 101
BanII G_rGCy'C
Cuts at: 1391 2479 3081 3740 3915 4133 4224 4983 5444
Size: 1088 602 659 175 218 91 759 461
Cuts at: 5444 1391
Size: 4072
Fragments arranged by size:
4072 1088 759 659 602 461 218 175
91
BbeI G_GCGC'C
Cuts at: 3966 3966
Size: 8125
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2097 2264 928
Size: 971 198 167 6789
Fragments arranged by size:
6789 971 198 167
BbvCI CC'TCA_GC
Cuts at: 3723 3723
Size: 8125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4967 5001 6316 6350 1903
Size: 34 3030 34 1315 34 3678
Fragments arranged by size:
3678 3030 1315 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2642 2900 6017 7227 2642
Size: 258 3117 1210 3540
Fragments arranged by size:
3540 3117 1210 258
BclI T'GATC_A
Cuts at: 1548 2161 2428 4489 1548
Size: 613 267 2061 5184
Fragments arranged by size:
5184 2061 613 267
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2775 3597 6736
Size: 107 122 71 631 605 1103 822 3139
Cuts at: 6736 136
Size: 1525
Fragments arranged by size:
3139 1525 1103 822 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3237 4061 8120 3237
Size: 824 4059 3242
Fragments arranged by size:
4059 3242 824
BlpI GC'TnA_GC
Cuts at: 3621 5542 3621
Size: 1921 6204
Bme1580I G_kGCm'C
Cuts at: 1790 2561 2789 3290 3298 3556 3740 3814 3915
Size: 771 228 501 8 258 184 74 101
Cuts at: 3915 4133 4273 4373 6186 7342 1790
Size: 218 140 100 1813 1156 2573
Fragments arranged by size:
2573 1813 1156 771 501 258 228 218
184 140 101 100 74 8
BmgBI CAC'GTC
Cuts at: 1318 2620 2749 2868 4828 1318
Size: 1302 129 119 1960 4615
Fragments arranged by size:
4615 1960 1302 129 119
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2122 2443 3459
Size: 416 53 83 399 24 705 321 1016
Cuts at: 3459 3981 4135 4455 4782 4801 4944 5856 6814
Size: 522 154 320 327 19 143 912 958
Cuts at: 6814 442
Size: 1753
Fragments arranged by size:
1753 1016 958 912 705 522 416 399
327 321 320 154 143 83 53 24
19
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8125
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4260 4260
Size: 8125
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2395 2775 3171 6785 1239
Size: 1156 380 396 3614 2579
Fragments arranged by size:
3614 2579 1156 396 380
Bpu10I CC'TnA_GC
Cuts at: 2030 3723 5072 5373 2030
Size: 1693 1349 301 4782
Fragments arranged by size:
4782 1693 1349 301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6250 7115 7413 6250
Size: 865 298 6962
Fragments arranged by size:
6962 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2963 6788 882
Size: 617 1464 3825 2219
Fragments arranged by size:
3825 2219 1464 617
BsaAI yAC'GTr
Cuts at: 493 1204 3007 3549 3603 4149 5916 493
Size: 711 1803 542 54 546 1767 2702
Fragments arranged by size:
2702 1803 1767 711 546 542 54
BsaBI GATnn'nnATC
Cuts at: 2427 8119 2427
Size: 5692 2433
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3963 5281 5932 6314
Size: 53 83 186 936 2430 1318 651 382
Cuts at: 6314 6949 275
Size: 635 1451
Fragments arranged by size:
2430 1451 1318 936 651 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2654 6558 6956 7230 7377 7788 2654
Size: 3904 398 274 147 411 2991
Fragments arranged by size:
3904 2991 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3190 3220 1178
Size: 30 165 30 1787 30 6083
Fragments arranged by size:
6083 1787 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2748 1186
Size: 1562 6563
BseYI C'CCAG_C
Cuts at: 2391 3732 7328 7824 2391
Size: 1341 3596 496 2692
Fragments arranged by size:
3596 2692 1341 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2167 2431 3529 4012 4032 4213 4915
Size: 573 431 264 1098 483 20 181 702
Cuts at: 4915 1163
Size: 4373
Fragments arranged by size:
4373 1098 702 573 483 431 264 181
20
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5575 6336 7364 1540
Size: 275 186 3574 761 1028 2301
Fragments arranged by size:
3574 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3290 3556 3814 4273 4983 6186 6271 7342 3290
Size: 266 258 459 710 1203 85 1071 4073
Fragments arranged by size:
4073 1203 1071 710 459 266 258 85
BsiWI C'GTAC_G
Cuts at: 3603 3603
Size: 8125
BsmI GAATG_Cn'
Cuts at: 2636 2765 2849 5636 5729 2636
Size: 129 84 2787 93 5032
Fragments arranged by size:
5032 2787 129 93 84
BsmBI CGTCTCn'nnnn_
Cuts at: 5288 5288
Size: 8125
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2479 2561 2789 3081 3290 3298 3556
Size: 399 689 82 228 292 209 8 258
Cuts at: 3556 3740 3814 3915 4133 4224 4273 4373 4983
Size: 184 74 101 218 91 49 100 610
Cuts at: 4983 5444 6186 6271 7342 1391
Size: 461 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 689 610 461 399 292
258 228 218 209 184 101 100 91
85 82 74 49 8
BspEI T'CCGG_A
Cuts at: 7788 7788
Size: 8125
BspHI T'CATG_A
Cuts at: 6014 7744 6014
Size: 1730 6395
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2618 2981 3551 4034 4126 4439 5349 5480
Size: 1774 363 570 483 92 313 910 131
Cuts at: 5480 844
Size: 3489
Fragments arranged by size:
3489 1774 910 570 483 363 313 131
92
BsrBI CCG'CTC
Cuts at: 1394 1823 3012 6012 1394
Size: 429 1189 3000 3507
Fragments arranged by size:
3507 3000 1189 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2893 3670 3979 4578 4756 4940 6620
Size: 1183 1645 777 309 599 178 184 1680
Cuts at: 6620 6794 65
Size: 174 1396
Fragments arranged by size:
1680 1645 1396 1183 777 599 309 184
178 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2454 3929 4945 5164 6769 6956 1700
Size: 110 644 1475 1016 219 1605 187 2869
Fragments arranged by size:
2869 1605 1475 1016 644 219 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8125
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8125
BssSI C'ACGA_G
Cuts at: 2176 6185 7197 2176
Size: 4009 1012 3104
Fragments arranged by size:
4009 3104 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3038 4098 794
Size: 2244 1060 4821
Fragments arranged by size:
4821 2244 1060
BstBI TT'CG_AA
Cuts at: 6929 6929
Size: 8125
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8125
BstXI CCAn_nnnn'nTGG
Cuts at: 2063 3647 4316 4445 2063
Size: 1584 669 129 5743
Fragments arranged by size:
5743 1584 669 129
BstYI r'GATC_y
Cuts at: 1972 2671 3237 3843 4061 5293 5455 5822 6207
Size: 699 566 606 218 1232 162 367 385
Cuts at: 6207 6224 7676 7762 7774 8120 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1232 699 606 566 385 367
346 218 162 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3072 3072
Size: 8125
BtgI C'CryG_G
Cuts at: 513 1065 2004 2347 3663 513
Size: 552 939 343 1316 4975
Fragments arranged by size:
4975 1316 939 552 343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2436 3854 487
Size: 683 56 1210 1418 4758
Fragments arranged by size:
4758 1418 1210 683 56
BtsI GCAGTG_nn'
Cuts at: 2443 4099 4328 5425 5641 6435 6455 2443
Size: 1656 229 1097 216 794 20 4113
Fragments arranged by size:
4113 1656 1097 794 229 216 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8125
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8090
DraI TTT'AAA
Cuts at: 2206 5429 2206
Size: 3223 4902
DraIII CAC_nnn'GTG
Cuts at: 2705 2793 3442 4809 2705
Size: 88 649 1367 6021
Fragments arranged by size:
6021 1367 649 88
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7132 809
Size: 507 5816 1802
Fragments arranged by size:
5816 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5572 6461 8090
Size: 54 1425 50 275 18 3742 889 1629
Cuts at: 8090 8
Size: 43
Fragments arranged by size:
3742 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5572 1537
Size: 275 3760 4090
Fragments arranged by size:
4090 3760 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4619 4904 5167 6055 1353
Size: 3266 285 263 888 3423
Fragments arranged by size:
3423 3266 888 285 263
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6712 7086 7232 387
Size: 1064 138 5123 374 146 1280
Fragments arranged by size:
5123 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4981 4981
Size: 8125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2395 2775 3171 4359 5193 6188 6785
Size: 393 763 380 396 1188 834 995 597
Cuts at: 6785 7572 7840 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1188 995 834 787 763 597 396
393 380 268
EcoO109I rG'GnC_Cy
Cuts at: 2438 2946 3540 3736 3737 3912 4129 4449 4916
Size: 508 594 196 1 175 217 320 467
Cuts at: 4916 2438
Size: 5647
Fragments arranged by size:
5647 594 508 467 320 217 196 175
1
EcoRI G'AATT_C
Cuts at: 5041 5434 5812 5041
Size: 393 378 7354
Fragments arranged by size:
7354 393 378
FspI TGC'GCA
Cuts at: 6631 6631
Size: 8125
HaeII r_GCGC'y
Cuts at: 1678 2536 3052 3628 3966 4182 7272 1678
Size: 858 516 576 338 216 3090 2531
Fragments arranged by size:
3090 2531 858 576 516 338 216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2227 2259 3190 3222 4474
Size: 32 135 32 655 32 931 32 1252
Cuts at: 4474 4506 5144 5176 5348 5380 6766 6798 6840
Size: 32 638 32 172 32 1386 32 42
Cuts at: 6840 6872 1373
Size: 32 2626
Fragments arranged by size:
2626 1386 1252 931 655 638 172 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2986 4831 5469 5715 669
Size: 988 281 1048 1845 638 246 3079
Fragments arranged by size:
3079 1845 1048 988 638 281 246
HindIII A'AGCT_T
Cuts at: 748 3225 748
Size: 2477 5648
HpaI GTT'AAC
Cuts at: 5715 5715
Size: 8125
KasI G'GCGC_C
Cuts at: 3962 3962
Size: 8125
KpnI G_GTAC'C
Cuts at: 5450 5450
Size: 8125
MfeI C'AATT_G
Cuts at: 5724 5724
Size: 8125
MluI A'CGCG_T
Cuts at: 8001 8001
Size: 8125
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4064 4492 7239 7423 1305
Size: 2759 428 2747 184 2007
Fragments arranged by size:
2759 2747 2007 428 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6303
Fragments arranged by size:
6303 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3064 3502 3559 3645 4276 4749 6083
Size: 648 1898 438 57 86 631 473 1334
Cuts at: 6083 6442 6601 518
Size: 359 159 2042
Fragments arranged by size:
2042 1898 1334 648 631 473 438 359
159 86 57
MspA1I CmG'CkG
Cuts at: 1606 2547 2637 3246 3678 3909 5541 6218 7366
Size: 941 90 609 432 231 1632 677 1148
Cuts at: 7366 7611 1606
Size: 245 2120
Fragments arranged by size:
2120 1632 1148 941 677 609 432 245
231 90
NaeI GCC'GGC
Cuts at: 4947 5166 4947
Size: 219 7906
NarI GG'CG_CC
Cuts at: 3963 3963
Size: 8125
NcoI C'CATG_G
Cuts at: 513 1065 2004 2347 513
Size: 552 939 343 6291
Fragments arranged by size:
6291 939 552 343
NdeI CA'TA_TG
Cuts at: 387 4384 5092 5185 5299 387
Size: 3997 708 93 114 3213
Fragments arranged by size:
3997 3213 708 114 93
NgoMIV G'CCGG_C
Cuts at: 4945 5164 4945
Size: 219 7906
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8125
NotI GC'GGCC_GC
Cuts at: 5572 5572
Size: 8125
NspI r_CATG'y
Cuts at: 1132 1229 3021 3204 3501 3888 4675 5483 7028
Size: 97 1792 183 297 387 787 808 1545
Cuts at: 7028 1132
Size: 2229
Fragments arranged by size:
2229 1792 1545 808 787 387 297 183
97
PasI CC'CwG_GG
Cuts at: 1348 4904 1348
Size: 3556 4569
PciI A'CATG_T
Cuts at: 3200 7024 3200
Size: 3824 4301
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8125
PfoI T'CCnGG_A
Cuts at: 2290 2902 3208 2290
Size: 612 306 7207
Fragments arranged by size:
7207 612 306
PmeI GTTT'AAAC
Cuts at: 5429 5429
Size: 8125
PmlI CAC'GTG
Cuts at: 3007 3549 4149 3007
Size: 542 600 6983
Fragments arranged by size:
6983 600 542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4726 4758 6194 6226 7707 7739 4726
Size: 32 1436 32 1481 32 5112
Fragments arranged by size:
5112 1481 1436 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2438 2946 3540 4449 2438
Size: 508 594 909 6114
Fragments arranged by size:
6114 909 594 508
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8125
PsiI TTA'TAA
Cuts at: 5695 5695
Size: 8125
PspOMI G'GGCC_C
Cuts at: 3736 3911 4129 3736
Size: 175 218 7732
Fragments arranged by size:
7732 218 175
PstI C_TGCA'G
Cuts at: 830 3245 3638 3878 4178 4463 5477 830
Size: 2415 393 240 300 285 1014 3478
Fragments arranged by size:
3478 2415 1014 393 300 285 240
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8125
PvuII CAG'CTG
Cuts at: 3246 3246
Size: 8125
SacI G_AGCT'C
Cuts at: 4983 4983
Size: 8125
SalI G'TCGA_C
Cuts at: 1936 5467 1936
Size: 3531 4594
SanDI GG'GwC_CC
Cuts at: 2438 2438
Size: 8125
SbfI CC_TGCA'GG
Cuts at: 5477 5477
Size: 8125
ScaI AGT'ACT
Cuts at: 4543 6373 8085 4543
Size: 1830 1712 4583
Fragments arranged by size:
4583 1830 1712
SfcI C'TryA_G
Cuts at: 826 1045 3241 3634 3874 4174 4459 5241 5473
Size: 219 2196 393 240 300 285 782 232
Cuts at: 5473 6608 7289 7480 826
Size: 1135 681 191 1471
Fragments arranged by size:
2196 1471 1135 782 681 393 300 285
240 232 219 191
SfoI GGC'GCC
Cuts at: 3964 3964
Size: 8125
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8125
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8125
SmaI CCC'GGG
Cuts at: 1872 5452 1872
Size: 3580 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4528 6229 7130 7392 820
Size: 197 926 2585 1701 901 262 1553
Fragments arranged by size:
2585 1701 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5916 493
Size: 5423 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8125
SphI G_CATG'C
Cuts at: 1132 3021 4675 5483 1132
Size: 1889 1654 808 3774
Fragments arranged by size:
3774 1889 1654 808
SspI AAT'ATT
Cuts at: 5 52 6049 5
Size: 47 5997 2081
Fragments arranged by size:
5997 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8125
StyI C'CwwG_G
Cuts at: 513 1065 2004 2347 5257 5564 513
Size: 552 939 343 2910 307 3074
Fragments arranged by size:
3074 2910 939 552 343 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4820 6152 6169 6322 6507 6952 325
Size: 1255 3240 1332 17 153 185 445 1498
Fragments arranged by size:
3240 1498 1332 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3416 4325 4541
Size: 274 80 33 51 1443 1439 909 216
Cuts at: 4541 5116 6371 8083 96
Size: 575 1255 1712 138
Fragments arranged by size:
1712 1443 1439 1255 909 575 274 216
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3804 3836 3994 4026 4078 4110 8085 8117 3804
Size: 32 158 32 52 32 3975 32 3812
Fragments arranged by size:
3975 3812 158 52 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2618 1316
Size: 1302 6823
XbaI T'CTAG_A
Cuts at: 5461 5461
Size: 8125
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2468 2597 2855 4122 2468
Size: 129 258 1267 6471
Fragments arranged by size:
6471 1267 258 129
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8125
XmaI C'CCGG_G
Cuts at: 1870 5450 1870
Size: 3580 4545
XmnI GAAnn'nnTTC
Cuts at: 5041 5176 6254 5041
Size: 135 1078 6912
Fragments arranged by size:
6912 1078 135
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5933 276
Size: 53 83 186 936 4399 2468
Fragments arranged by size:
4399 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BstZ17I EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PspXI PsrI RsrII
SacII SapI SexAI SfiI SrfI SwaI