Restriction map for
pFN21AA1775
(Circular) (Six-base) MAPSORT of: pFN21AA1775.seq Check: 7429 from: 1 to: 7282
pFN21AA1775 7282 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5092 278
Size: 53 83 186 936 3556 2468
Fragments arranged by size:
3556 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2166 4625 1937
Size: 229 2459 4594
Fragments arranged by size:
4594 2459 229
Acc65I G'GTAC_C
Cuts at: 4603 4603
Size: 7282
AclI AA'CG_TT
Cuts at: 1563 5409 5782 1563
Size: 3846 373 3063
Fragments arranged by size:
3846 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2822 3985 5345 6729 6997 1632
Size: 1190 1163 1360 1384 268 1917
Fragments arranged by size:
1917 1384 1360 1190 1163 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7085
AflIII A'CryG_T
Cuts at: 1203 3858 6181 7158 1203
Size: 2655 2323 977 1327
Fragments arranged by size:
2655 2323 1327 977
AgeI A'CCGG_T
Cuts at: 6113 6113
Size: 7282
AhdI GACnn_n'nnGTC
Cuts at: 6011 6011
Size: 7282
AleI CACnn'nnGTG
Cuts at: 1166 2565 3491 4139 1166
Size: 1399 926 648 4309
Fragments arranged by size:
4309 1399 926 648
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7282
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2522 2554 3726 3758 2522
Size: 32 1172 32 6046
Fragments arranged by size:
6046 1172 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2189 2501 2533 3391 4040 5025 6597 2189
Size: 312 32 858 649 985 1572 2874
Fragments arranged by size:
2874 1572 985 858 649 312 32
ApaLI G'TGCA_C
Cuts at: 4562 5339 6495 4562
Size: 777 1156 5349
Fragments arranged by size:
5349 1156 777
ApoI r'AATT_y
Cuts at: 1493 2844 3185 3901 4591 4817 4969 7078 1493
Size: 1351 341 716 690 226 152 2109 1697
Fragments arranged by size:
2109 1697 1351 716 690 341 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2889 5836 160
Size: 872 1857 2947 1606
Fragments arranged by size:
2947 1857 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7282
AvaI C'yCGr_G
Cuts at: 1870 1943 2438 4369 4607 1870
Size: 73 495 1931 238 4545
Fragments arranged by size:
4545 1931 495 238 73
BamHI G'GATC_C
Cuts at: 3496 4612 4979 3496
Size: 1116 367 5799
Fragments arranged by size:
5799 1116 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2398 4124 4405 4603 6058
Size: 325 217 627 611 1726 281 198 1455
Cuts at: 6058 618
Size: 1842
Fragments arranged by size:
1842 1726 1455 627 611 325 281 217
198
BanII G_rGCy'C
Cuts at: 1391 2792 2927 2984 2996 3470 3852 3992 4061
Size: 1401 135 57 12 474 382 140 69
Cuts at: 4061 4378 4536 4601 1391
Size: 317 158 65 4072
Fragments arranged by size:
4072 1401 474 382 317 158 140 135
69 65 57 12
BbeI G_GCGC'C
Cuts at: 2402 4409 2402
Size: 2007 5275
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2731 3758 928
Size: 971 832 1027 4452
Fragments arranged by size:
4452 1027 971 832
BbvCI CC'TCA_GC
Cuts at: 3618 3684 3618
Size: 66 7216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5473 5507 1903
Size: 34 3536 34 3678
Fragments arranged by size:
3678 3536 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5174 6384 5174
Size: 1210 6072
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7282
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2030 4213 5893
Size: 107 122 71 631 605 358 2183 1680
Cuts at: 5893 136
Size: 1525
Fragments arranged by size:
2183 1680 1525 631 605 358 122 107
71
BglII A'GATC_T
Cuts at: 3888 7277 3888
Size: 3389 3893
BlpI GC'TnA_GC
Cuts at: 4699 4699
Size: 7282
Bme1580I G_kGCm'C
Cuts at: 1790 2750 4566 5343 6499 1790
Size: 960 1816 777 1156 2573
Fragments arranged by size:
2573 1816 1156 960 777
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7282
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3011 3555 3588
Size: 416 53 83 399 24 1594 544 33
Cuts at: 3588 3767 4420 5013 5971 442
Size: 179 653 593 958 1753
Fragments arranged by size:
1753 1594 958 653 593 544 416 399
179 83 53 33 24
BmtI G_CTAG'C
Cuts at: 1056 3609 1056
Size: 2553 4729
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4066 4066
Size: 7282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2523 2564 2921 2922 3454 5942 1239
Size: 1284 41 357 1 532 2488 2579
Fragments arranged by size:
2579 2488 1284 532 357 41 1
Bpu10I CC'TnA_GC
Cuts at: 2061 3618 3684 2061
Size: 1557 66 5659
Fragments arranged by size:
5659 1557 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3128 3881 4004 4256 4520 5407 6272 6570 3128
Size: 753 123 252 264 887 865 298 3840
Fragments arranged by size:
3840 887 865 753 298 264 252 123
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2190 2817 4037 5945 882
Size: 617 691 627 1220 1908 2219
Fragments arranged by size:
2219 1908 1220 691 627 617
BsaAI yAC'GTr
Cuts at: 493 1204 2164 5073 493
Size: 711 960 2909 2702
Fragments arranged by size:
2909 2702 960 711
BsaBI GATnn'nnATC
Cuts at: 4028 7276 4028
Size: 3248 4034
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2399 4406 5089 5471
Size: 53 83 186 936 866 2007 683 382
Cuts at: 5471 6106 275
Size: 635 1451
Fragments arranged by size:
2007 1451 936 866 683 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5715 6113 6387 6534 6945 5715
Size: 398 274 147 411 6052
Fragments arranged by size:
6052 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2189 2219 2496 2522 2526
Size: 30 165 30 786 30 277 26 4
Cuts at: 2526 2552 3656 3686 3974 4004 1178
Size: 26 1104 30 288 30 4456
Fragments arranged by size:
4456 1104 786 288 277 165 30 30
30 30 30 26 26 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7282
BseYI C'CCAG_C
Cuts at: 2228 2453 2585 2931 2995 3594 4235 4397 4409
Size: 225 132 346 64 599 641 162 12
Cuts at: 4409 6485 6981 2228
Size: 2076 496 2529
Fragments arranged by size:
2529 2076 641 599 496 346 225 162
132 64 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2519 3911 3992 1163
Size: 573 783 1392 81 4453
Fragments arranged by size:
4453 1392 783 573 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4732 5493 6521 1540
Size: 275 186 2731 761 1028 2301
Fragments arranged by size:
2731 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3114 4224 4286 4378 4536 4566 5343 5428 6499
Size: 1110 62 92 158 30 777 85 1071
Cuts at: 6499 3114
Size: 3897
Fragments arranged by size:
3897 1110 1071 777 158 92 85 62
30
BsmI GAATG_Cn'
Cuts at: 4793 4886 4793
Size: 93 7189
BsmBI CGTCTCn'nnnn_
Cuts at: 4450 4450
Size: 7282
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2750 2792 2927 2984 2996 3114 3470
Size: 399 960 42 135 57 12 118 356
Cuts at: 3470 3852 3992 4061 4224 4286 4378 4536 4566
Size: 382 140 69 163 62 92 158 30
Cuts at: 4566 4601 5343 5428 6499 1391
Size: 35 742 85 1071 2174
Fragments arranged by size:
2174 1071 960 742 399 382 356 163
158 140 135 118 92 85 69 62
57 42 35 30 12
BspEI T'CCGG_A
Cuts at: 6945 6945
Size: 7282
BspHI T'CATG_A
Cuts at: 5171 6901 5171
Size: 1730 5552
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2458 2541 4637 844
Size: 1614 83 2096 3489
Fragments arranged by size:
3489 2096 1614 83
BsrBI CCG'CTC
Cuts at: 1394 1823 3075 3912 5169 1394
Size: 429 1252 837 1257 3507
Fragments arranged by size:
3507 1257 1252 837 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5777 5951 65
Size: 1183 4529 174 1396
Fragments arranged by size:
4529 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2016 4121 5926 6113 1700
Size: 110 206 2105 1805 187 2869
Fragments arranged by size:
2869 2105 1805 206 187 110
BsrGI T'GTAC_A
Cuts at: 96 2962 96
Size: 2866 4416
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7282
BssSI C'ACGA_G
Cuts at: 5342 6354 5342
Size: 1012 6270
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2533 794
Size: 1739 5543
BstBI TT'CG_AA
Cuts at: 6086 6086
Size: 7282
BstEII G'GTnAC_C
Cuts at: 1184 2377 1184
Size: 1193 6089
BstXI CCAn_nnnn'nTGG
Cuts at: 3478 3820 4141 4498 3478
Size: 342 321 357 6262
Fragments arranged by size:
6262 357 342 321
BstYI r'GATC_y
Cuts at: 1972 3129 3496 3888 4612 4979 5364 5381 6833
Size: 1157 367 392 724 367 385 17 1452
Cuts at: 6833 6919 6931 7277 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1157 724 392 385 367 367
346 86 17 12
BstZ17I GTA'TAC
Cuts at: 2167 2167
Size: 7282
Bsu36I CC'TnA_GG
Cuts at: 2190 2190
Size: 7282
BtgI C'CryG_G
Cuts at: 513 1065 2550 2631 2694 2814 4062 4107 4128
Size: 552 1485 81 63 120 1248 45 21
Cuts at: 4128 513
Size: 3667
Fragments arranged by size:
3667 1485 1248 552 120 81 63 45
21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6543
Fragments arranged by size:
6543 683 56
BtsI GCAGTG_nn'
Cuts at: 3624 3698 4798 5592 5612 3624
Size: 74 1100 794 20 5294
Fragments arranged by size:
5294 1100 794 74 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7282
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3252 3287 529
Size: 35 2688 35 4524
Fragments arranged by size:
4524 2688 35 35
DraI TTT'AAA
Cuts at: 4586 4586
Size: 7282
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2096 6289 809
Size: 507 780 4193 1802
Fragments arranged by size:
4193 1802 780 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2355 4119 4131
Size: 54 1425 50 275 18 525 1764 12
Cuts at: 4131 4143 4729 5618 7247 8
Size: 12 586 889 1629 43
Fragments arranged by size:
1764 1629 1425 889 586 525 275 54
50 43 18 12 12
EagI C'GGCC_G
Cuts at: 1537 1812 4729 1537
Size: 275 2917 4090
Fragments arranged by size:
4090 2917 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2280 3563 4550 5212 1353
Size: 927 1283 987 662 3423
Fragments arranged by size:
3423 1283 987 927 662
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5869 6243 6389 387
Size: 1064 138 4280 374 146 1280
Fragments arranged by size:
4280 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4376 4534 4376
Size: 158 7124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2523 2564 2822 2921 2922 3454 3985
Size: 393 891 41 258 99 1 532 531
Cuts at: 3985 5345 5942 6729 6997 1239
Size: 1360 597 787 268 1524
Fragments arranged by size:
1524 1360 891 787 597 532 531 393
268 258 99 41 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2138 2138
Size: 7282
EcoO109I rG'GnC_Cy
Cuts at: 2610 3139 3206 3509 3768 4201 2610
Size: 529 67 303 259 433 5691
Fragments arranged by size:
5691 529 433 303 259 67
EcoRI G'AATT_C
Cuts at: 2844 3901 4591 4969 2844
Size: 1057 690 378 5157
Fragments arranged by size:
5157 1057 690 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4594 4594
Size: 7282
FspI TGC'GCA
Cuts at: 5788 5788
Size: 7282
HaeII r_GCGC'y
Cuts at: 1678 2014 2402 4409 6429 1678
Size: 336 388 2007 2020 2531
Fragments arranged by size:
2531 2020 2007 388 336
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2187 2189 2219 2221 2483
Size: 32 135 32 615 2 30 2 262
Cuts at: 2483 2496 2515 2528 3974 4006 5923 5955 5997
Size: 13 19 13 1446 32 1917 32 42
Cuts at: 5997 6029 1373
Size: 32 2626
Fragments arranged by size:
2626 1917 1446 615 262 135 42 32
32 32 32 32 30 19 13 13
2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2254 4518 4626 4872 669
Size: 988 281 316 2264 108 246 3079
Fragments arranged by size:
3079 2264 988 316 281 246 108
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7282
HpaI GTT'AAC
Cuts at: 4872 4872
Size: 7282
KasI G'GCGC_C
Cuts at: 2398 4405 2398
Size: 2007 5275
KpnI G_GTAC'C
Cuts at: 4607 4607
Size: 7282
MfeI C'AATT_G
Cuts at: 4881 4881
Size: 7282
MluI A'CGCG_T
Cuts at: 7158 7158
Size: 7282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3189 6396 6580 1305
Size: 1884 3207 184 2007
Fragments arranged by size:
3207 2007 1884 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4133 4145 10
Size: 54 1425 343 2301 12 3147
Fragments arranged by size:
3147 2301 1425 343 54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 2565 2630 3491 4139 5240 5599 5758
Size: 648 1399 65 861 648 1101 359 159
Cuts at: 5758 518
Size: 2042
Fragments arranged by size:
2042 1399 1101 861 648 648 359 159
65
MspA1I CmG'CkG
Cuts at: 1606 2565 2999 3199 3284 3371 3491 4698 5375
Size: 959 434 200 85 87 120 1207 677
Cuts at: 5375 6523 6768 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1207 1148 959 677 434 245 200
120 87 85
NaeI GCC'GGC
Cuts at: 4123 4123
Size: 7282
NarI GG'CG_CC
Cuts at: 2399 4406 2399
Size: 2007 5275
NcoI C'CATG_G
Cuts at: 513 1065 2814 4062 4128 513
Size: 552 1749 1248 66 3667
Fragments arranged by size:
3667 1749 1248 552 66
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7282
NgoMIV G'CCGG_C
Cuts at: 4121 4121
Size: 7282
NheI G'CTAG_C
Cuts at: 1052 3605 1052
Size: 2553 4729
NotI GC'GGCC_GC
Cuts at: 4729 4729
Size: 7282
NspI r_CATG'y
Cuts at: 1132 1229 2963 4640 6185 1132
Size: 97 1734 1677 1545 2229
Fragments arranged by size:
2229 1734 1677 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2035 3121 3210 3461 3513 3600 1348
Size: 687 1086 89 251 52 87 5030
Fragments arranged by size:
5030 1086 687 251 89 87 52
PciI A'CATG_T
Cuts at: 6181 6181
Size: 7282
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2482 3391 3578 4176 1108
Size: 1374 909 187 598 4214
Fragments arranged by size:
4214 1374 909 598 187
PfoI T'CCnGG_A
Cuts at: 2412 2612 3390 4052 2412
Size: 200 778 662 5642
Fragments arranged by size:
5642 778 662 200
PmeI GTTT'AAAC
Cuts at: 4586 4586
Size: 7282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2522 2554 5351 5383 6864 6896 2522
Size: 32 2797 32 1481 32 2908
Fragments arranged by size:
2908 2797 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2610 3206 3509 3768 4201 2610
Size: 596 303 259 433 5691
Fragments arranged by size:
5691 596 433 303 259
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7282
PsiI TTA'TAA
Cuts at: 4852 4852
Size: 7282
PstI C_TGCA'G
Cuts at: 830 2529 4634 830
Size: 1699 2105 3478
Fragments arranged by size:
3478 2105 1699
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7282
PvuII CAG'CTG
Cuts at: 2999 3199 3284 3491 2999
Size: 200 85 207 6790
Fragments arranged by size:
6790 207 200 85
SacI G_AGCT'C
Cuts at: 4378 4536 4378
Size: 158 7124
SalI G'TCGA_C
Cuts at: 1936 4624 1936
Size: 2688 4594
SanDI GG'GwC_CC
Cuts at: 3206 3768 3206
Size: 562 6720
SapI GCTCTTCn'nnn_
Cuts at: 2280 2280
Size: 7282
SbfI CC_TGCA'GG
Cuts at: 4634 4634
Size: 7282
ScaI AGT'ACT
Cuts at: 5530 7242 5530
Size: 1712 5570
SexAI A'CCwGG_T
Cuts at: 3819 3819
Size: 7282
SfcI C'TryA_G
Cuts at: 826 1045 2151 2525 2851 4630 5765 6446 6637
Size: 219 1106 374 326 1779 1135 681 191
Cuts at: 6637 826
Size: 1471
Fragments arranged by size:
1779 1471 1135 1106 681 374 326 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2030 2030
Size: 7282
SfoI GGC'GCC
Cuts at: 2400 4407 2400
Size: 2007 5275
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7282
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7282
SmaI CCC'GGG
Cuts at: 1872 4609 1872
Size: 2737 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3143 3896 4019 4271 4535 5386
Size: 197 926 1200 753 123 252 264 851
Cuts at: 5386 6287 6549 820
Size: 901 262 1553
Fragments arranged by size:
1553 1200 926 901 851 753 264 262
252 197 123
SnaBI TAC'GTA
Cuts at: 493 5073 493
Size: 4580 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7282
SphI G_CATG'C
Cuts at: 1132 4640 1132
Size: 3508 3774
SspI AAT'ATT
Cuts at: 5 52 3530 5206 5
Size: 47 3478 1676 2081
Fragments arranged by size:
3478 2081 1676 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7282
StyI C'CwwG_G
Cuts at: 513 1065 2640 2814 3978 4062 4086 4128 4721
Size: 552 1575 174 1164 84 24 42 593
Cuts at: 4721 513
Size: 3074
Fragments arranged by size:
3074 1575 1164 593 552 174 84 42
24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2396 3129 3585 3608 3854 4100 4411
Size: 1255 816 733 456 23 246 246 311
Cuts at: 4411 5309 5326 5479 5664 6109 325
Size: 898 17 153 185 445 1498
Fragments arranged by size:
1498 1255 898 816 733 456 445 311
246 246 185 153 23 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2962 3672 5528
Size: 274 80 33 51 1443 985 710 1856
Cuts at: 5528 7240 96
Size: 1712 138
Fragments arranged by size:
1856 1712 1443 985 710 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3225 3257 7242 7274 3225
Size: 32 3985 32 3233
Fragments arranged by size:
3985 3233 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3407 1316
Size: 2091 5191
XbaI T'CTAG_A
Cuts at: 4618 4618
Size: 7282
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2116 3031 3575 3820 3913 4105 2116
Size: 915 544 245 93 192 5293
Fragments arranged by size:
5293 915 544 245 192 93
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7282
XmaI C'CCGG_G
Cuts at: 1870 4607 1870
Size: 2737 4545
XmnI GAAnn'nnTTC
Cuts at: 5411 5411
Size: 7282
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5090 276
Size: 53 83 186 936 3556 2468
Fragments arranged by size:
3556 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI
DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SrfI SwaI