Restriction map for
pFN21AA1773
(Circular) (Six-base) MAPSORT of: pFN21AA1773.seq Check: 7573 from: 1 to: 14599
pFN21AA1773 14599 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2821 3421 3790 5539 5923 6262 8569 9133 11263
Size: 600 369 1749 384 339 2307 564 2130
Cuts at: 11263 11426 2821
Size: 163 5994
Fragments arranged by size:
5994 2307 2130 1749 600 564 384 369
339 163
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5422 6930 12409 278
Size: 53 83 186 936 3886 1508 5479 2468
Fragments arranged by size:
5479 3886 2468 1508 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 8670 11942 1937
Size: 6733 3272 4594
Fragments arranged by size:
6733 4594 3272
Acc65I G'GTAC_C
Cuts at: 2458 5680 11920 2458
Size: 3222 6240 5137
Fragments arranged by size:
6240 5137 3222
AclI AA'CG_TT
Cuts at: 1563 12726 13099 1563
Size: 11163 373 3063
Fragments arranged by size:
11163 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4716 5234 5469 5631 7915 7989 8953 9253
Size: 3084 518 235 162 2284 74 964 300
Cuts at: 9253 9501 12662 14046 14314 1632
Size: 248 3161 1384 268 1917
Fragments arranged by size:
3161 3084 2284 1917 1384 964 518 300
268 248 235 162 74
AfeI AGC'GCT
Cuts at: 6366 6493 6804 6366
Size: 127 311 14161
Fragments arranged by size:
14161 311 127
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 14402
AflIII A'CryG_T
Cuts at: 1203 6655 6739 13498 14475 1203
Size: 5452 84 6759 977 1327
Fragments arranged by size:
6759 5452 1327 977 84
AgeI A'CCGG_T
Cuts at: 13430 13430
Size: 14599
AhdI GACnn_n'nnGTC
Cuts at: 13328 13328
Size: 14599
AleI CACnn'nnGTG
Cuts at: 1166 4112 4427 6110 7832 8039 8159 9305 9465
Size: 2946 315 1683 1722 207 120 1146 160
Cuts at: 9465 10145 10175 11624 1166
Size: 680 30 1449 4141
Fragments arranged by size:
4141 2946 1722 1683 1449 1146 680 315
207 160 120 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4130 4631 7022 9676 1184
Size: 2946 501 2391 2654 6107
Fragments arranged by size:
6107 2946 2654 2391 501
AlwNI CAG_nnn'CTG
Cuts at: 2519 2618 2722 5062 5231 5542 5591 5654 5926
Size: 99 104 2340 169 311 49 63 272
Cuts at: 5926 6005 6029 6907 7814 7838 9052 9530 9722
Size: 79 24 878 907 24 1214 478 192
Cuts at: 9722 9770 9839 10259 10391 10754 10919 10969 11048
Size: 48 69 420 132 363 165 50 79
Cuts at: 11048 11117 11348 11861 12342 13914 2519
Size: 69 231 513 481 1572 3204
Fragments arranged by size:
3204 2340 1572 1214 907 878 513 481
478 420 363 311 272 231 192 169
165 132 104 99 79 79 69 69
63 50 49 48 24 24
ApaI G_GGCC'C
Cuts at: 2716 3954 4878 5096 6690 7129 7821 8571 8958
Size: 1238 924 218 1594 439 692 750 387
Cuts at: 8958 9012 10894 11340 2716
Size: 54 1882 446 5975
Fragments arranged by size:
5975 1882 1594 1238 924 750 692 446
439 387 218 54
ApaLI G'TGCA_C
Cuts at: 3919 6093 9407 10056 12656 13812 3919
Size: 2174 3314 649 2600 1156 4706
Fragments arranged by size:
4706 3314 2600 2174 1156 649
ApoI r'AATT_y
Cuts at: 1493 11908 12134 12286 14395 1493
Size: 10415 226 152 2109 1697
Fragments arranged by size:
10415 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 13153 160
Size: 872 12121 1606
Fragments arranged by size:
12121 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 14599
AvaI C'yCGr_G
Cuts at: 1870 1943 2442 3495 4326 4503 4638 4914 6128
Size: 73 499 1053 831 177 135 276 1214
Cuts at: 6128 6711 7485 8097 8211 9442 9648 9813 11682
Size: 583 774 612 114 1231 206 165 1869
Cuts at: 11682 11924 1870
Size: 242 4545
Fragments arranged by size:
4545 1869 1231 1214 1053 831 774 612
583 499 276 242 206 177 165 135
114 73
AvrII C'CTAG_G
Cuts at: 10876 10876
Size: 14599
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2598 2631 2598
Size: 33 14566
BamHI G'GATC_C
Cuts at: 3499 4148 4498 5326 5731 7093 8962 11929 12296
Size: 649 350 828 405 1362 1869 2967 367
Cuts at: 12296 3499
Size: 5802
Fragments arranged by size:
5802 2967 1869 1362 828 649 405 367
350
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2113 2120 2375 2458 2480
Size: 325 217 627 326 7 255 83 22
Cuts at: 2480 3512 3548 3773 4414 4421 4468 5039 5680
Size: 1032 36 225 641 7 47 571 641
Cuts at: 5680 5995 6119 6134 6940 7282 7427 7594 7688
Size: 315 124 15 806 342 145 167 94
Cuts at: 7688 7985 8369 9221 9974 10072 10447 10478 10532
Size: 297 384 852 753 98 375 31 54
Cuts at: 10532 10630 10792 11511 11920 13375 618
Size: 98 162 719 409 1455 1842
Fragments arranged by size:
1842 1455 1032 852 806 753 719 641
641 627 571 409 384 375 342 326
325 315 297 255 225 217 167 162
145 124 98 98 94 83 54 47
36 31 22 15 7 7
BanII G_rGCy'C
Cuts at: 1391 2054 2716 3022 3038 3447 3732 3954 4759
Size: 663 662 306 16 409 285 222 805
Cuts at: 4759 4878 4928 5096 5570 6062 6081 6357 6690
Size: 119 50 168 474 492 19 276 333
Cuts at: 6690 7129 7187 7722 7821 8571 8768 8783 8853
Size: 439 58 535 99 750 197 15 70
Cuts at: 8853 8882 8958 9012 9382 9951 9971 10606 10894
Size: 29 76 54 370 569 20 635 288
Cuts at: 10894 11340 11384 11475 11918 1391
Size: 446 44 91 443 4072
Fragments arranged by size:
4072 805 750 663 662 635 569 535
492 474 446 443 439 409 370 333
306 288 285 276 222 197 168 119
99 91 76 70 58 54 50 44
29 20 19 16 15
BbeI G_GCGC'C
Cuts at: 3552 6944 7692 3552
Size: 3392 748 10459
Fragments arranged by size:
10459 3392 748
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4704 5457 6598 6631 9489 9586 11533
Size: 971 2805 753 1141 33 2858 97 1947
Cuts at: 11533 928
Size: 3994
Fragments arranged by size:
3994 2858 2805 1947 1141 971 753 97
33
BbvCI CC'TCA_GC
Cuts at: 3366 3630 4118 4290 5944 6030 6231 7472 9618
Size: 264 488 172 1654 86 201 1241 2146
Cuts at: 9618 10697 10908 11743 11836 11842 3366
Size: 1079 211 835 93 6 6123
Fragments arranged by size:
6123 2146 1654 1241 1079 835 488 264
211 201 172 93 86 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2180 2214 4532 4566 10187
Size: 34 50 34 159 34 2318 34 5621
Cuts at: 10187 10221 11237 11271 12790 12824 1903
Size: 34 1016 34 1519 34 3678
Fragments arranged by size:
5621 3678 2318 1519 1016 159 50 34
34 34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 12491 13701 12491
Size: 1210 13389
BclI T'GATC_A
Cuts at: 1548 8163 10105 11142 1548
Size: 6615 1942 1037 5005
Fragments arranged by size:
6615 5005 1942 1037
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3834 4256 4873
Size: 107 122 71 631 605 2162 422 617
Cuts at: 4873 5139 6685 9906 11171 11456 11730 13210 136
Size: 266 1546 3221 1265 285 274 1480 1525
Fragments arranged by size:
3221 2162 1546 1525 1480 1265 631 617
605 422 285 274 266 122 107 71
BglII A'GATC_T
Cuts at: 5971 8718 14594 5971
Size: 2747 5876 5976
Fragments arranged by size:
5976 5876 2747
BlpI GC'TnA_GC
Cuts at: 6488 6843 8190 8386 8782 8942 9861 11504 12016
Size: 355 1347 196 396 160 919 1643 512
Cuts at: 12016 6488
Size: 9071
Fragments arranged by size:
9071 1643 1347 919 512 396 355 196
160
Bme1580I G_kGCm'C
Cuts at: 1790 2273 2716 3776 3923 3954 4093 4132 4327
Size: 483 443 1060 147 31 139 39 195
Cuts at: 4327 4620 4878 5096 6097 6137 6690 7129 7267
Size: 293 258 218 1001 40 553 439 138
Cuts at: 7267 7821 8571 8958 9012 9411 9655 10060 10320
Size: 554 750 387 54 399 244 405 260
Cuts at: 10320 10473 10483 10633 10894 11340 11516 11655 12660
Size: 153 10 150 261 446 176 139 1005
Cuts at: 12660 13816 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1060 1005 1001 750 554 553
483 446 443 439 405 399 387 293
261 260 258 244 218 195 176 153
150 147 139 139 138 54 40 39
31 10
BmgBI CAC'GTC
Cuts at: 1318 2731 3388 6418 7036 1318
Size: 1413 657 3030 618 8881
Fragments arranged by size:
8881 3030 1413 657 618
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2643 4314 4583
Size: 416 53 83 399 24 1226 1671 269
Cuts at: 4583 9592 9665 10793 10979 11403 11500 12330 13288
Size: 5009 73 1128 186 424 97 830 958
Cuts at: 13288 442
Size: 1753
Fragments arranged by size:
5009 1753 1671 1226 1128 958 830 424
416 399 269 186 97 83 73 53
24
BmtI G_CTAG'C
Cuts at: 1056 8220 1056
Size: 7164 7435
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2674 3052 5812 7741 11336 2674
Size: 378 2760 1929 3595 5937
Fragments arranged by size:
5937 3595 2760 1929 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2409 2516 2586 2595 2603 2699 4298 4613
Size: 1170 107 70 9 8 96 1599 315
Cuts at: 4613 4922 4928 5165 5505 5682 5903 6819 7298
Size: 309 6 237 340 177 221 916 479
Cuts at: 7298 7437 7545 7677 7736 7749 8631 9366 9536
Size: 139 108 132 59 13 882 735 170
Cuts at: 9536 10149 10437 10724 11070 11888 13259 1239
Size: 613 288 287 346 818 1371 2579
Fragments arranged by size:
2579 1599 1371 1170 916 882 818 735
613 479 346 340 315 309 288 287
237 221 177 170 139 132 108 107
96 70 59 13 9 8 6
Bpu10I CC'TnA_GC
Cuts at: 2463 2619 3027 3245 3366 3441 3630 4118 4290
Size: 156 408 218 121 75 189 488 172
Cuts at: 4290 5944 6030 6231 7472 7823 9618 9931 9970
Size: 1654 86 201 1241 351 1795 313 39
Cuts at: 9970 10697 10908 11378 11743 11836 11842 2463
Size: 727 211 470 365 93 6 5220
Fragments arranged by size:
5220 1795 1654 1241 727 488 470 408
365 351 313 218 211 201 189 172
156 121 93 86 75 39 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3343 3940 3983 6775 9182 11546 12724 13589 13887
Size: 597 43 2792 2407 2364 1178 865 298
Cuts at: 13887 3343
Size: 4055
Fragments arranged by size:
4055 2792 2407 2364 1178 865 597 298
43
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2564 6509 9363 10001 13262 882
Size: 617 1065 3945 2854 638 3261 2219
Fragments arranged by size:
3945 3261 2854 2219 1065 638 617
BsaAI yAC'GTr
Cuts at: 493 1204 6703 6850 8654 8668 9412 10507 12390
Size: 711 5499 147 1804 14 744 1095 1883
Cuts at: 12390 493
Size: 2702
Fragments arranged by size:
5499 2702 1883 1804 1095 744 711 147
14
BsaBI GATnn'nnATC
Cuts at: 8804 14593 8804
Size: 5789 8810
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2524 3549 5419 6390
Size: 53 83 186 936 991 1025 1870 971
Cuts at: 6390 6927 6941 7068 7689 12406 12788 13423 275
Size: 537 14 127 621 4717 382 635 1451
Fragments arranged by size:
4717 1870 1451 1025 991 971 936 635
621 537 382 186 127 83 53 14
BsaWI w'CCGG_w
Cuts at: 2889 3875 13032 13430 13704 13851 14262 2889
Size: 986 9157 398 274 147 411 3226
Fragments arranged by size:
9157 3226 986 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2209 2239 3551 3581 5818
Size: 30 165 30 806 30 1312 30 2237
Cuts at: 5818 5848 6863 6893 7383 7413 8011 8041 8979
Size: 30 1015 30 490 30 598 30 938
Cuts at: 8979 9009 10213 10243 10833 10863 1178
Size: 30 1204 30 590 30 4914
Fragments arranged by size:
4914 2237 1312 1204 1015 938 806 598
590 490 165 30 30 30 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 2217 3317 3673 3830 4041 5892 7163
Size: 991 40 1100 356 157 211 1851 1271
Cuts at: 7163 7182 8466 11167 11345 11480 11483 11772 1186
Size: 19 1284 2701 178 135 3 289 4013
Fragments arranged by size:
4013 2701 1851 1284 1271 1100 991 356
289 211 178 157 135 40 19 3
BseYI C'CCAG_C
Cuts at: 2017 2101 2108 2135 2519 3031 3777 4621 4808
Size: 84 7 27 384 512 746 844 187
Cuts at: 4808 5989 7721 7772 7814 7925 8643 8767 9623
Size: 1181 1732 51 42 111 718 124 856
Cuts at: 9623 10114 10208 10247 10373 11027 11341 11410 11587
Size: 491 94 39 126 654 314 69 177
Cuts at: 11587 11759 13802 14298 2017
Size: 172 2043 496 2318
Fragments arranged by size:
2318 2043 1732 1181 856 844 746 718
654 512 496 491 384 314 187 177
172 126 124 111 94 84 69 51
42 39 27 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3129 3368 4292 4674 5090 5567 5573
Size: 573 1393 239 924 382 416 477 6
Cuts at: 5573 5786 5951 6290 6302 6603 6683 6720 6770
Size: 213 165 339 12 301 80 37 50
Cuts at: 6770 7815 7827 9434 10529 11328 11370 11876 1163
Size: 1045 12 1607 1095 799 42 506 3886
Fragments arranged by size:
3886 1607 1393 1095 1045 924 799 573
506 477 416 382 339 301 239 213
165 80 50 42 37 12 12 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6556 6821 12049 12810 13838 1540
Size: 275 186 4555 265 5228 761 1028 2301
Fragments arranged by size:
5228 4555 2301 1028 761 275 265 186
BsiHKAI G_wGCw'C
Cuts at: 3923 5884 6097 6849 8853 9168 9411 9971 10060
Size: 1961 213 752 2004 315 243 560 89
Cuts at: 10060 10335 11007 11475 12660 12745 13816 3923
Size: 275 672 468 1185 85 1071 4706
Fragments arranged by size:
4706 2004 1961 1185 1071 752 672 560
468 315 275 243 213 89 85
BsmI GAATG_Cn'
Cuts at: 8248 8319 12110 12203 8248
Size: 71 3791 93 10644
Fragments arranged by size:
10644 3791 93 71
BsmBI CGTCTCn'nnnn_
Cuts at: 7075 9202 7075
Size: 2127 12472
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2273 2716 3022 3038 3447 3732
Size: 399 264 219 443 306 16 409 285
Cuts at: 3732 3776 3923 3954 4093 4132 4327 4620 4759
Size: 44 147 31 139 39 195 293 139
Cuts at: 4759 4878 4928 5096 5570 5884 6062 6081 6097
Size: 119 50 168 474 314 178 19 16
Cuts at: 6097 6137 6357 6690 6849 7129 7187 7267 7722
Size: 40 220 333 159 280 58 80 455
Cuts at: 7722 7821 8571 8768 8783 8853 8882 8958 9012
Size: 99 750 197 15 70 29 76 54
Cuts at: 9012 9168 9382 9411 9655 9951 9971 10060 10320
Size: 156 214 29 244 296 20 89 260
Cuts at: 10320 10335 10473 10483 10606 10633 10894 11007 11340
Size: 15 138 10 123 27 261 113 333
Cuts at: 11340 11384 11475 11516 11655 11918 12660 12745 13816
Size: 44 91 41 139 263 742 85 1071
Cuts at: 13816 1391
Size: 2174
Fragments arranged by size:
2174 1071 750 742 474 455 443 409
399 333 333 314 306 296 293 285
280 264 263 261 260 244 220 219
214 197 195 178 168 159 156 147
139 139 139 138 123 119 113 99
91 89 85 80 76 70 58 54
50 44 44 41 40 39 31 29
29 27 20 19 16 16 15 15
10
BspEI T'CCGG_A
Cuts at: 3875 14262 3875
Size: 10387 4212
BspHI T'CATG_A
Cuts at: 5884 7642 9070 12488 14218 5884
Size: 1758 1428 3418 1730 6265
Fragments arranged by size:
6265 3418 1758 1730 1428
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2446 2821 3421 3790 5037 5539 5923 6262
Size: 1602 375 600 369 1247 502 384 339
Cuts at: 6262 6892 8569 9133 9495 10620 10815 11263 11426
Size: 630 1677 564 362 1125 195 448 163
Cuts at: 11426 11508 11954 844
Size: 82 446 3489
Fragments arranged by size:
3489 1677 1602 1247 1125 630 600 564
502 448 446 384 375 369 362 339
195 163 82
BsrBI CCG'CTC
Cuts at: 1394 1823 2658 2956 5854 5983 8076 11712 12486
Size: 429 835 298 2898 129 2093 3636 774
Cuts at: 12486 1394
Size: 3507
Fragments arranged by size:
3636 3507 2898 2093 835 774 429 298
129
BsrDI GCAATG_nn'
Cuts at: 65 1248 2075 13094 13268 65
Size: 1183 827 11019 174 1396
Fragments arranged by size:
11019 1396 1183 827 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6454 6555 7691 8007 8647 11459 13243
Size: 110 4644 101 1136 316 640 2812 1784
Cuts at: 13243 13430 1700
Size: 187 2869
Fragments arranged by size:
4644 2869 2812 1784 1136 640 316 187
110 101
BsrGI T'GTAC_A
Cuts at: 96 5205 5763 6369 96
Size: 5109 558 606 8326
Fragments arranged by size:
8326 5109 606 558
BssHII G'CGCG_C
Cuts at: 1925 6211 6213 6252 6314 6473 6587 6599 1925
Size: 4286 2 39 62 159 114 12 9925
Fragments arranged by size:
9925 4286 159 114 62 39 12 2
BssSI C'ACGA_G
Cuts at: 2505 3006 9366 9396 9911 10175 12659 13671 2505
Size: 501 6360 30 515 264 2484 1012 3433
Fragments arranged by size:
6360 3433 2484 1012 515 501 264 30
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2846 10064 11117 794
Size: 2052 7218 1053 4276
Fragments arranged by size:
7218 4276 2052 1053
BstBI TT'CG_AA
Cuts at: 6192 13403 6192
Size: 7211 7388
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 14599
BstXI CCAn_nnnn'nTGG
Cuts at: 2124 4267 5338 6683 2124
Size: 2143 1071 1345 10040
Fragments arranged by size:
10040 2143 1345 1071
BstYI r'GATC_y
Cuts at: 1972 3499 3878 4148 4498 5326 5731 5971 6721
Size: 1527 379 270 350 828 405 240 750
Cuts at: 6721 7093 8718 8962 11133 11929 12296 12681 12698
Size: 372 1625 244 2171 796 367 385 17
Cuts at: 12698 14150 14236 14248 14594 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2171 1977 1625 1527 1452 828 796 750
405 385 379 372 367 350 346 270
244 240 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3453 3583 3768 4879 5035 6157 7287 9840 10260
Size: 130 185 1111 156 1122 1130 2553 420
Cuts at: 10260 3453
Size: 7792
Fragments arranged by size:
7792 2553 1130 1122 1111 420 185 156
130
BtgI C'CryG_G
Cuts at: 513 1065 4005 4064 4153 4830 4984 6081 6206
Size: 552 2940 59 89 677 154 1097 125
Cuts at: 6206 6690 6810 6874 8417 9373 9733 10688 10761
Size: 484 120 64 1543 956 360 955 73
Cuts at: 10761 10824 11065 11191 11476 11625 513
Size: 63 241 126 285 149 3487
Fragments arranged by size:
3487 2940 1543 1097 956 955 677 552
484 360 285 241 154 149 126 125
120 89 73 64 63 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 7872 487
Size: 683 56 6646 7214
Fragments arranged by size:
7214 6646 683 56
BtsI GCAGTG_nn'
Cuts at: 2355 2735 3356 3761 4615 4722 4748 5043 5232
Size: 380 621 405 854 107 26 295 189
Cuts at: 5232 5610 5826 7803 8227 9032 9187 9351 10958
Size: 378 216 1977 424 805 155 164 1607
Cuts at: 10958 11346 11619 12115 12909 12929 2355
Size: 388 273 496 794 20 4025
Fragments arranged by size:
4025 1977 1607 854 805 794 621 496
424 405 388 380 378 295 273 216
189 164 155 107 26 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 14599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 8113 8148 529
Size: 35 7549 35 6980
Fragments arranged by size:
7549 6980 35 35
DraI TTT'AAA
Cuts at: 11903 11903
Size: 14599
DraIII CAC_nnn'GTG
Cuts at: 5156 5866 5879 5156
Size: 710 13 13876
Fragments arranged by size:
13876 710 13
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 13606 809
Size: 507 12290 1802
Fragments arranged by size:
12290 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2280 2957 3651
Size: 54 1425 50 275 18 450 677 694
Cuts at: 3651 4248 5955 6772 6818 6894 7436 7666 7953
Size: 597 1707 817 46 76 542 230 287
Cuts at: 7953 8121 8134 8748 8977 10149 10489 11098 11163
Size: 168 13 614 229 1172 340 609 65
Cuts at: 11163 11457 11532 11576 12046 12935 14564 8
Size: 294 75 44 470 889 1629 43
Fragments arranged by size:
1707 1629 1425 1172 889 817 694 677
614 609 597 542 470 450 340 294
287 275 230 229 168 76 75 65
54 50 46 44 43 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 6818 12046 1537
Size: 275 5006 5228 4090
Fragments arranged by size:
5228 5006 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2042 4517 7125 8117 10573 11028 12529 1353
Size: 689 2475 2608 992 2456 455 1501 3423
Fragments arranged by size:
3423 2608 2475 2456 1501 992 689 455
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8603 13186 13560 13706 387
Size: 1064 138 7014 4583 374 146 1280
Fragments arranged by size:
7014 4583 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 8851 9969 11473 8851
Size: 1118 1504 11977
Fragments arranged by size:
11977 1504 1118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2409 2516 2586 2595 2603 2699 4298
Size: 393 777 107 70 9 8 96 1599
Cuts at: 4298 4613 4716 4922 4928 5165 5234 5469 5505
Size: 315 103 206 6 237 69 235 36
Cuts at: 5505 5631 5682 5903 6819 7298 7437 7545 7677
Size: 126 51 221 916 479 139 108 132
Cuts at: 7677 7736 7749 7915 7989 8631 8953 9253 9366
Size: 59 13 166 74 642 322 300 113
Cuts at: 9366 9501 9536 10149 10437 10724 11070 11888 12662
Size: 135 35 613 288 287 346 818 774
Cuts at: 12662 13259 14046 14314 1239
Size: 597 787 268 1524
Fragments arranged by size:
1599 1524 916 818 787 777 774 642
613 597 479 393 346 322 315 300
288 287 268 237 235 221 206 166
139 135 132 126 113 108 107 103
96 74 70 69 59 51 36 35
13 9 8 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3452 3507 5546 5811 6668 8364 8576 9146 10241
Size: 55 2039 265 857 1696 212 570 1095
Cuts at: 10241 11202 3452
Size: 961 6849
Fragments arranged by size:
6849 2039 1696 1095 961 857 570 265
212 55
EcoO109I rG'GnC_Cy
Cuts at: 2058 2712 2719 3870 3950 3951 4874 4875 5059
Size: 654 7 1151 80 1 923 1 184
Cuts at: 5059 5092 5093 5408 5665 5986 6715 6904 7126
Size: 33 1 315 257 321 729 189 222
Cuts at: 7126 7297 7381 7617 7818 7922 8093 8567 8568
Size: 171 84 236 201 104 171 474 1
Cuts at: 8568 8921 8955 9008 9009 9291 9644 10256 10721
Size: 353 34 53 1 282 353 612 465
Cuts at: 10721 11054 11336 11337 11831 2058
Size: 333 282 1 494 4826
Fragments arranged by size:
4826 1151 923 729 654 612 494 474
465 353 353 333 321 315 282 282
257 236 222 201 189 184 171 171
104 84 80 53 34 33 7 1
1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 11908 12286 11908
Size: 378 14221
EcoRV GAT'ATC
Cuts at: 10768 10768
Size: 14599
FspI TGC'GCA
Cuts at: 7739 13105 7739
Size: 5366 9233
HaeII r_GCGC'y
Cuts at: 1678 3552 6074 6166 6332 6338 6368 6461 6488
Size: 1874 2522 92 166 6 30 93 27
Cuts at: 6488 6495 6806 6944 7692 13746 1678
Size: 7 311 138 748 6054 2531
Fragments arranged by size:
6054 2531 2522 1874 748 311 166 138
93 92 30 27 7 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3332 3364 3566 3598 4133
Size: 32 135 32 1760 32 202 32 535
Cuts at: 4133 4165 4202 4234 4320 4352 4514 4546 4754
Size: 32 37 32 86 32 162 32 208
Cuts at: 4754 4786 4910 4942 5018 5050 5387 5419 5518
Size: 32 124 32 76 32 337 32 99
Cuts at: 5518 5550 6140 6172 6863 6895 7169 7201 7252
Size: 32 590 32 691 32 274 32 51
Cuts at: 7252 7284 7383 7415 8091 8123 8591 8623 8900
Size: 32 99 32 676 32 468 32 277
Cuts at: 8900 8932 8979 9011 9200 9232 9953 9985 10000
Size: 32 47 32 189 32 721 32 15
Cuts at: 10000 10032 13240 13272 13314 13346 1373
Size: 32 3208 32 42 32 2626
Fragments arranged by size:
3208 2626 1760 721 691 676 590 535
468 337 277 274 208 202 189 162
135 124 99 99 86 76 51 47
42 37 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3400 4230 4780 6931 7200 9277
Size: 988 281 1462 830 550 2151 269 2077
Cuts at: 9277 11943 12189 669
Size: 2666 246 3079
Fragments arranged by size:
3079 2666 2151 2077 1462 988 830 550
281 269 246
HindIII A'AGCT_T
Cuts at: 748 3958 7081 10342 748
Size: 3210 3123 3261 5005
Fragments arranged by size:
5005 3261 3210 3123
HpaI GTT'AAC
Cuts at: 4780 7200 12189 4780
Size: 2420 4989 7190
Fragments arranged by size:
7190 4989 2420
KasI G'GCGC_C
Cuts at: 3548 6940 7688 3548
Size: 3392 748 10459
Fragments arranged by size:
10459 3392 748
KpnI G_GTAC'C
Cuts at: 2462 5684 11924 2462
Size: 3222 6240 5137
Fragments arranged by size:
6240 5137 3222
MfeI C'AATT_G
Cuts at: 10083 12198 10083
Size: 2115 12484
MluI A'CGCG_T
Cuts at: 6655 6739 14475 6655
Size: 84 7736 6779
Fragments arranged by size:
7736 6779 84
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3603 4566 6366 6804 8152 9835 10221 11548
Size: 2298 963 1800 438 1348 1683 386 1327
Cuts at: 11548 13713 13897 1305
Size: 2165 184 2007
Fragments arranged by size:
2298 2165 2007 1800 1683 1348 1327 963
438 386 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2282 3653 4250 7668 7955
Size: 54 1425 343 450 1371 597 3418 287
Cuts at: 7955 8123 8750 10491 11165 11534 10
Size: 168 627 1741 674 369 3075
Fragments arranged by size:
3418 3075 1741 1425 1371 674 627 597
450 369 343 287 168 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3170 3207 3909 4112 4427 6110 7832
Size: 648 2004 37 702 203 315 1683 1722
Cuts at: 7832 7850 8039 8159 9305 9465 10145 10175 11094
Size: 18 189 120 1146 160 680 30 919
Cuts at: 11094 11624 12557 12916 13075 518
Size: 530 933 359 159 2042
Fragments arranged by size:
2042 2004 1722 1683 1146 933 919 702
680 648 530 359 315 203 189 160
159 120 37 30 18
MspA1I CmG'CkG
Cuts at: 1606 2174 2262 2338 2677 2977 3640 3980 4112
Size: 568 88 76 339 300 663 340 132
Cuts at: 4112 4276 5443 5923 5993 6029 6083 6325 6692
Size: 164 1167 480 70 36 54 242 367
Cuts at: 6692 7471 7745 7811 9043 9049 9418 9554 9601
Size: 779 274 66 1232 6 369 136 47
Cuts at: 9601 9728 10641 11675 11747 12015 12692 13840 14085
Size: 127 913 1034 72 268 677 1148 245
Cuts at: 14085 1606
Size: 2120
Fragments arranged by size:
2120 1232 1167 1148 1034 913 779 677
663 568 480 369 367 340 339 300
274 268 245 242 164 136 132 127
88 76 72 70 66 54 47 36
6
NaeI GCC'GGC
Cuts at: 6456 8649 11461 6456
Size: 2193 2812 9594
Fragments arranged by size:
9594 2812 2193
NarI GG'CG_CC
Cuts at: 3549 6941 7689 3549
Size: 3392 748 10459
Fragments arranged by size:
10459 3392 748
NcoI C'CATG_G
Cuts at: 513 1065 4005 4153 513
Size: 552 2940 148 10959
Fragments arranged by size:
10959 2940 552 148
NdeI CA'TA_TG
Cuts at: 387 387
Size: 14599
NgoMIV G'CCGG_C
Cuts at: 6454 8647 11459 6454
Size: 2193 2812 9594
Fragments arranged by size:
9594 2812 2193
NheI G'CTAG_C
Cuts at: 1052 8216 1052
Size: 7164 7435
NotI GC'GGCC_GC
Cuts at: 12046 12046
Size: 14599
NspI r_CATG'y
Cuts at: 1132 1229 3059 5600 6302 7208 9875 10193 11957
Size: 97 1830 2541 702 906 2667 318 1764
Cuts at: 11957 13502 1132
Size: 1545 2229
Fragments arranged by size:
2667 2541 2229 1830 1764 1545 906 702
318 97
PasI CC'CwG_GG
Cuts at: 1348 2536 4730 5363 8252 8274 8844 11827 1348
Size: 1188 2194 633 2889 22 570 2983 4120
Fragments arranged by size:
4120 2983 2889 2194 1188 633 570 22
PciI A'CATG_T
Cuts at: 13498 13498
Size: 14599
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2791 2992 3188 4177 4900 5156 5509 5926
Size: 1683 201 196 989 723 256 353 417
Cuts at: 5926 7256 8030 8533 9997 10498 11048 11417 11530
Size: 1330 774 503 1464 501 550 369 113
Cuts at: 11530 1108
Size: 4177
Fragments arranged by size:
4177 1683 1464 1330 989 774 723 550
503 501 417 369 353 256 201 196
113
PfoI T'CCnGG_A
Cuts at: 5698 8706 10092 10736 5698
Size: 3008 1386 644 9561
Fragments arranged by size:
9561 3008 1386 644
PmeI GTTT'AAAC
Cuts at: 11903 11903
Size: 14599
PmlI CAC'GTG
Cuts at: 6703 8654 9412 10507 6703
Size: 1951 758 1095 10795
Fragments arranged by size:
10795 1951 1095 758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 12668 12700 14181 14213 12668
Size: 32 1481 32 13054
Fragments arranged by size:
13054 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3870 5059 5408 5986 6715 6904 7297 7381 7617
Size: 1189 349 578 729 189 393 84 236
Cuts at: 7617 8921 9291 10256 11054 11831 3870
Size: 1304 370 965 798 777 6638
Fragments arranged by size:
6638 1304 1189 965 798 777 729 578
393 370 349 236 189 84
PshAI GACnn'nnGTC
Cuts at: 1613 3868 1613
Size: 2255 12344
PsiI TTA'TAA
Cuts at: 12169 12169
Size: 14599
PspOMI G'GGCC_C
Cuts at: 2712 3950 4874 5092 6686 7125 7817 8567 8954
Size: 1238 924 218 1594 439 692 750 387
Cuts at: 8954 9008 10890 11336 2712
Size: 54 1882 446 5975
Fragments arranged by size:
5975 1882 1594 1238 924 750 692 446
439 387 218 54
PstI C_TGCA'G
Cuts at: 830 2362 2457 2949 3801 5643 5724 5833 7615
Size: 1532 95 492 852 1842 81 109 1782
Cuts at: 7615 7632 7750 8181 8439 8580 9048 9423 9727
Size: 17 118 431 258 141 468 375 304
Cuts at: 9727 10965 11122 11680 11951 830
Size: 1238 157 558 271 3478
Fragments arranged by size:
3478 1842 1782 1532 1238 852 558 492
468 431 375 304 271 258 157 141
118 109 95 81 17
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 14599
PvuII CAG'CTG
Cuts at: 2677 3640 3980 4112 4276 5443 5923 6029 7471
Size: 963 340 132 164 1167 480 106 1442
Cuts at: 7471 7745 7811 9043 9049 9418 9601 9728 11675
Size: 274 66 1232 6 369 183 127 1947
Cuts at: 11675 11747 2677
Size: 72 5529
Fragments arranged by size:
5529 1947 1442 1232 1167 963 480 369
340 274 183 164 132 127 106 72
66 6
SacI G_AGCT'C
Cuts at: 8853 9971 11475 8853
Size: 1118 1504 11977
Fragments arranged by size:
11977 1504 1118
SacII CC_GC'GG
Cuts at: 6084 6693 6084
Size: 609 13990
SalI G'TCGA_C
Cuts at: 1936 11941 1936
Size: 10005 4594
SanDI GG'GwC_CC
Cuts at: 5986 6715 7381 7617 9291 11831 5986
Size: 729 666 236 1674 2540 8754
Fragments arranged by size:
8754 2540 1674 729 666 236
SapI GCTCTTCn'nnn_
Cuts at: 2042 2042
Size: 14599
SbfI CC_TGCA'GG
Cuts at: 2457 3801 5643 7615 11951 2457
Size: 1344 1842 1972 4336 5105
Fragments arranged by size:
5105 4336 1972 1842 1344
ScaI AGT'ACT
Cuts at: 12847 14559 12847
Size: 1712 12887
SexAI A'CCwGG_T
Cuts at: 4456 9147 11267 4456
Size: 4691 2120 7788
Fragments arranged by size:
7788 4691 2120
SfcI C'TryA_G
Cuts at: 826 1045 2358 2453 2672 2945 3797 4307 4382
Size: 219 1313 95 219 273 852 510 75
Cuts at: 4382 4632 5428 5639 5720 5829 7611 7628 7634
Size: 250 796 211 81 109 1782 17 6
Cuts at: 7634 7746 8082 8177 8314 8435 8576 8626 9044
Size: 112 336 95 137 121 141 50 418
Cuts at: 9044 9419 9522 9562 9723 10596 10961 11118 11323
Size: 375 103 40 161 873 365 157 205
Cuts at: 11323 11676 11947 13082 13763 13954 826
Size: 353 271 1135 681 191 1471
Fragments arranged by size:
1782 1471 1313 1135 873 852 796 681
510 418 375 365 353 336 273 271
250 219 219 211 205 191 161 157
141 137 121 112 109 103 95 95
81 75 50 40 17 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 3834 4256 4873 9906 11171 3834
Size: 422 617 5033 1265 7262
Fragments arranged by size:
7262 5033 1265 617 422
SfoI GGC'GCC
Cuts at: 3550 6942 7690 3550
Size: 3392 748 10459
Fragments arranged by size:
10459 3392 748
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 14599
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 14599
SmaI CCC'GGG
Cuts at: 1872 4916 6713 7487 8213 9650 11684 11926 1872
Size: 3044 1797 774 726 1437 2034 242 4545
Fragments arranged by size:
4545 3044 2034 1797 1437 774 726 242
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3322 3955 3998 6790 9161 11561
Size: 197 926 1379 633 43 2792 2371 2400
Cuts at: 11561 12703 13604 13866 820
Size: 1142 901 262 1553
Fragments arranged by size:
2792 2400 2371 1553 1379 1142 926 901
633 262 197 43
SnaBI TAC'GTA
Cuts at: 493 12390 493
Size: 11897 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 14599
SphI G_CATG'C
Cuts at: 1132 6302 10193 11957 1132
Size: 5170 3891 1764 3774
Fragments arranged by size:
5170 3891 3774 1764
SrfI GCCC'GGGC
Cuts at: 11684 11684
Size: 14599
SspI AAT'ATT
Cuts at: 5 52 12523 5
Size: 47 12471 2081
Fragments arranged by size:
12471 2081 47
StuI AGG'CCT
Cuts at: 1517 2684 3996 4867 5462 9881 1517
Size: 1167 1312 871 595 4419 6235
Fragments arranged by size:
6235 4419 1312 1167 871 595
StyI C'CwwG_G
Cuts at: 513 1065 3508 3852 4005 4153 4206 4245 5890
Size: 552 2443 344 153 148 53 39 1645
Cuts at: 5890 7752 8559 9321 10836 10876 11076 12038 513
Size: 1862 807 762 1515 40 200 962 3074
Fragments arranged by size:
3074 2443 1862 1645 1515 962 807 762
552 344 200 153 148 53 40 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2661 3266 3572 3791 3845 4381 5148
Size: 1255 1081 605 306 219 54 536 767
Cuts at: 5148 5158 5412 6080 6525 7891 10349 10787 11404
Size: 10 254 668 445 1366 2458 438 617
Cuts at: 11404 11649 12626 12643 12796 12981 13426 325
Size: 245 977 17 153 185 445 1498
Fragments arranged by size:
2458 1498 1366 1255 1081 977 767 668
617 605 536 445 445 438 306 254
245 219 185 153 54 17 10
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 5205 5763
Size: 274 80 33 51 1443 258 2970 558
Cuts at: 5763 6369 6963 8363 9705 9778 10033 12845 14557
Size: 606 594 1400 1342 73 255 2812 1712
Cuts at: 14557 96
Size: 138
Fragments arranged by size:
2970 2812 1712 1443 1400 1342 606 594
558 274 258 255 138 80 73 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2209 2241 3551 3583 5694 5726 5818 5850 7866
Size: 32 1310 32 2111 32 92 32 2016
Cuts at: 7866 7898 8011 8043 9236 9268 10440 10472 10833
Size: 32 113 32 1193 32 1172 32 361
Cuts at: 10833 10865 14559 14591 2209
Size: 32 3694 32 2217
Fragments arranged by size:
3694 2217 2111 2016 1310 1193 1172 361
113 92 32 32 32 32 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3260 1316
Size: 1944 12655
XbaI T'CTAG_A
Cuts at: 9682 11935 9682
Size: 2253 12346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3515 3697 4151 4178 4327 5084 5157 5245 5480
Size: 182 454 27 149 757 73 88 235
Cuts at: 5480 8651 8704 11359 3515
Size: 3171 53 2655 6755
Fragments arranged by size:
6755 3171 2655 757 454 235 182 149
88 73 53 27
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 14599
XmaI C'CCGG_G
Cuts at: 1870 4914 6711 7485 8211 9648 11682 11924 1870
Size: 3044 1797 774 726 1437 2034 242 4545
Fragments arranged by size:
4545 3044 2034 1797 1437 774 726 242
XmnI GAAnn'nnTTC
Cuts at: 12728 12728
Size: 14599
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5420 6928 12407 276
Size: 53 83 186 936 3886 1508 5479 2468
Fragments arranged by size:
5479 3886 2468 1508 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BfrBI BsiWI BstZ17I FalI FseI FspAI
NruI NsiI PacI PspXI PsrI RsrII SwaI