Restriction map for
pFN21AA1746
(Circular) (Six-base) MAPSORT of: pFN21AA1746.seq Check: 5357 from: 1 to: 7987
pFN21AA1746 7987 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2737 2984 5154 2737
Size: 247 2170 5570
Fragments arranged by size:
5570 2170 247
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4728 5797 278
Size: 53 83 186 936 3192 1069 2468
Fragments arranged by size:
3192 2468 1069 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5330 1937
Size: 3393 4594
Acc65I G'GTAC_C
Cuts at: 3319 5308 3319
Size: 1989 5998
AclI AA'CG_TT
Cuts at: 1563 6114 6487 1563
Size: 4551 373 3063
Fragments arranged by size:
4551 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2210 2769 4178 5171 6050 7434 7702 1632
Size: 578 559 1409 993 879 1384 268 1917
Fragments arranged by size:
1917 1409 1384 993 879 578 559 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7790
AflIII A'CryG_T
Cuts at: 1203 5065 6886 7863 1203
Size: 3862 1821 977 1327
Fragments arranged by size:
3862 1821 1327 977
AgeI A'CCGG_T
Cuts at: 6818 6818
Size: 7987
AhdI GACnn_n'nnGTC
Cuts at: 2049 3748 6716 2049
Size: 1699 2968 3320
Fragments arranged by size:
3320 2968 1699
AleI CACnn'nnGTG
Cuts at: 1166 2867 3995 1166
Size: 1701 1128 5158
Fragments arranged by size:
5158 1701 1128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2701 4455 1184
Size: 1517 1754 4716
Fragments arranged by size:
4716 1754 1517
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2817 2849 4110 4142 4863 4895 2817
Size: 32 1261 32 721 32 5909
Fragments arranged by size:
5909 1261 721 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3139 4081 4966 5260 5730 7302 3139
Size: 942 885 294 470 1572 3824
Fragments arranged by size:
3824 1572 942 885 470 294
ApaI G_GGCC'C
Cuts at: 2896 2896
Size: 7987
ApaLI G'TGCA_C
Cuts at: 6044 7200 6044
Size: 1156 6831
ApoI r'AATT_y
Cuts at: 1493 2525 4554 5296 5522 5674 7783 1493
Size: 1032 2029 742 226 152 2109 1697
Fragments arranged by size:
2109 2029 1697 1032 742 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6541 160
Size: 872 5509 1606
Fragments arranged by size:
5509 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7987
AvaI C'yCGr_G
Cuts at: 1870 1943 2092 2101 3737 5312 1870
Size: 73 149 9 1636 1575 4545
Fragments arranged by size:
4545 1636 1575 149 73 9
AvrII C'CTAG_G
Cuts at: 2372 2372
Size: 7987
BamHI G'GATC_C
Cuts at: 2194 5317 5684 2194
Size: 3123 367 4497
Fragments arranged by size:
4497 3123 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2531 3319 5308 6763 618
Size: 325 217 627 744 788 1989 1455 1842
Fragments arranged by size:
1989 1842 1455 788 744 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2038 2093 2108 2405 2896 3855 4473 4985
Size: 647 55 15 297 491 959 618 512
Cuts at: 4985 5175 5306 1391
Size: 190 131 4072
Fragments arranged by size:
4072 959 647 618 512 491 297 190
131 55 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2438 2590 2879 4680 928
Size: 971 539 152 289 1801 4235
Fragments arranged by size:
4235 1801 971 539 289 152
BbvCI CC'TCA_GC
Cuts at: 3024 3024
Size: 7987
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6178 6212 1903
Size: 34 4241 34 3678
Fragments arranged by size:
4241 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3718 4198 5879 7089 3718
Size: 480 1681 1210 4616
Fragments arranged by size:
4616 1681 1210 480
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7987
BfrBI ATG'CAT
Cuts at: 3363 3363
Size: 7987
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2018 2097
Size: 107 122 71 631 605 337 9 79
Cuts at: 2097 4052 4274 5204 6598 136
Size: 1955 222 930 1394 1525
Fragments arranged by size:
1955 1525 1394 930 631 605 337 222
122 107 79 71 9
BglII A'GATC_T
Cuts at: 4315 7982 4315
Size: 3667 4320
BlpI GC'TnA_GC
Cuts at: 2087 2622 4082 5117 5404 2087
Size: 535 1460 1035 287 4670
Fragments arranged by size:
4670 1460 1035 535 287
Bme1580I G_kGCm'C
Cuts at: 1790 2896 3597 6048 7204 1790
Size: 1106 701 2451 1156 2573
Fragments arranged by size:
2573 2451 1156 1106 701
BmgBI CAC'GTC
Cuts at: 1318 2548 5182 1318
Size: 1230 2634 4123
Fragments arranged by size:
4123 2634 1230
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2702 2848 5194
Size: 416 53 83 399 24 1285 146 2346
Cuts at: 5194 5718 6676 442
Size: 524 958 1753
Fragments arranged by size:
2346 1753 1285 958 524 416 399 146
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7987
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3501 3874 3501
Size: 373 7614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2822 3239 3844 5189 5282 6647 1239
Size: 1583 417 605 1345 93 1365 2579
Fragments arranged by size:
2579 1583 1365 1345 605 417 93
Bpu10I CC'TnA_GC
Cuts at: 2037 2399 2812 3024 2037
Size: 362 413 212 7000
Fragments arranged by size:
7000 413 362 212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2092 2800 3618 4440 4628 4643 5114 6112 6977
Size: 708 818 822 188 15 471 998 865
Cuts at: 6977 7275 2092
Size: 298 2804
Fragments arranged by size:
2804 998 865 822 818 708 471 298
188 15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2941 4790 4861 6650 882
Size: 617 1442 1849 71 1789 2219
Fragments arranged by size:
2219 1849 1789 1442 617 71
BsaAI yAC'GTr
Cuts at: 493 1204 3388 5158 5778 493
Size: 711 2184 1770 620 2702
Fragments arranged by size:
2702 2184 1770 711 620
BsaBI GATnn'nnATC
Cuts at: 3653 7981 3653
Size: 4328 3659
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2911 4725 5794 6176
Size: 53 83 186 936 1378 1814 1069 382
Cuts at: 6176 6811 275
Size: 635 1451
Fragments arranged by size:
1814 1451 1378 1069 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2380 4207 6420 6818 7092 7239 7650 2380
Size: 1827 2213 398 274 147 411 2717
Fragments arranged by size:
2717 2213 1827 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2168 2198 4110 4140 4839
Size: 30 165 30 765 30 1912 30 699
Cuts at: 4839 4869 1178
Size: 30 4296
Fragments arranged by size:
4296 1912 765 699 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2768 3261 4134 1186
Size: 1582 493 873 5039
Fragments arranged by size:
5039 1582 873 493
BseYI C'CCAG_C
Cuts at: 2896 3087 4472 4978 7190 7686 2896
Size: 191 1385 506 2212 496 3197
Fragments arranged by size:
3197 2212 1385 506 496 191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2845 1163
Size: 573 1109 6305
Fragments arranged by size:
6305 1109 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5437 6198 7226 1540
Size: 275 186 3436 761 1028 2301
Fragments arranged by size:
3436 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4064 4921 6048 6133 7204 4064
Size: 857 1127 85 1071 4847
Fragments arranged by size:
4847 1127 1071 857 85
BsmI GAATG_Cn'
Cuts at: 3770 5498 5591 3770
Size: 1728 93 6166
Fragments arranged by size:
6166 1728 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2093 2108 2405 2896 3597 3855
Size: 399 248 55 15 297 491 701 258
Cuts at: 3855 4064 4473 4921 4985 5175 5306 6048 6133
Size: 209 409 448 64 190 131 742 85
Cuts at: 6133 7204 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 701 491 448 409 399
297 258 248 209 190 131 85 64
55 15
BspEI T'CCGG_A
Cuts at: 2380 7650 2380
Size: 5270 2717
BspHI T'CATG_A
Cuts at: 4354 5229 5876 7606 4354
Size: 875 647 1730 4735
Fragments arranged by size:
4735 1730 875 647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2669 2737 2984 5154 5342 844
Size: 1825 68 247 2170 188 3489
Fragments arranged by size:
3489 2170 1825 247 188 68
BsrBI CCG'CTC
Cuts at: 1394 1823 3919 5874 1394
Size: 429 2096 1955 3507
Fragments arranged by size:
3507 2096 1955 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4019 6482 6656 65
Size: 1183 2771 2463 174 1396
Fragments arranged by size:
2771 2463 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5284 6631 6818 1700
Size: 110 3474 1347 187 2869
Fragments arranged by size:
3474 2869 1347 187 110
BsrGI T'GTAC_A
Cuts at: 96 2589 96
Size: 2493 5494
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7987
BssSI C'ACGA_G
Cuts at: 6047 7059 6047
Size: 1012 6975
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4168 794
Size: 3374 4613
BstBI TT'CG_AA
Cuts at: 6791 6791
Size: 7987
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7987
BstXI CCAn_nnnn'nTGG
Cuts at: 2663 4099 4936 5068 2663
Size: 1436 837 132 5582
Fragments arranged by size:
5582 1436 837 132
BstYI r'GATC_y
Cuts at: 1972 2194 4315 4906 5134 5317 5684 6069 6086
Size: 222 2121 591 228 183 367 385 17
Cuts at: 6086 7538 7624 7636 7982 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2121 1977 1452 591 385 367 346 228
222 183 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4892 4892
Size: 7987
BtgI C'CryG_G
Cuts at: 513 1065 2004 2022 4065 4092 4255 4986 513
Size: 552 939 18 2043 27 163 731 3514
Fragments arranged by size:
3514 2043 939 731 552 163 27 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7248
Fragments arranged by size:
7248 683 56
BtsI GCAGTG_nn'
Cuts at: 3754 5503 6297 6317 3754
Size: 1749 794 20 5424
Fragments arranged by size:
5424 1749 794 20
ClaI AT'CG_AT
Cuts at: 1554 3280 1554
Size: 1726 6261
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7952
DraI TTT'AAA
Cuts at: 2154 5291 2154
Size: 3137 4850
DraIII CAC_nnn'GTG
Cuts at: 5161 5161
Size: 7987
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6994 809
Size: 507 5678 1802
Fragments arranged by size:
5678 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2252 4938 5022
Size: 54 1425 50 275 18 422 2686 84
Cuts at: 5022 5184 5434 6323 7952 8
Size: 162 250 889 1629 43
Fragments arranged by size:
2686 1629 1425 889 422 275 250 162
84 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5434 1537
Size: 275 3622 4090
Fragments arranged by size:
4090 3622 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2730 2745 3279 3903 4329 5208 5917 1353
Size: 1377 15 534 624 426 879 709 3423
Fragments arranged by size:
3423 1377 879 709 624 534 426 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2324 6574 6948 7094 387
Size: 1064 138 735 4250 374 146 1280
Fragments arranged by size:
4250 1280 1064 735 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2769 2822 3239 3844 4178 5171
Size: 393 578 559 53 417 605 334 993
Cuts at: 5171 5189 5282 6050 6647 7434 7702 1239
Size: 18 93 768 597 787 268 1524
Fragments arranged by size:
1524 993 787 768 605 597 578 559
417 393 334 268 93 53 18
EcoNI CCTnn'n_nnAGG
Cuts at: 4891 4891
Size: 7987
EcoO109I rG'GnC_Cy
Cuts at: 2605 2775 2893 2605
Size: 170 118 7699
Fragments arranged by size:
7699 170 118
EcoRI G'AATT_C
Cuts at: 5296 5674 5296
Size: 378 7609
EcoRV GAT'ATC
Cuts at: 2671 2671
Size: 7987
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3368 3889 4547 4655 4669 3368
Size: 521 658 108 14 6686
Fragments arranged by size:
6686 658 521 108 14
FspI TGC'GCA
Cuts at: 6493 6493
Size: 7987
HaeII r_GCGC'y
Cuts at: 1678 5124 7134 1678
Size: 3446 2010 2531
Fragments arranged by size:
3446 2531 2010
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2035 2067 2872 2904 2913
Size: 32 135 32 463 32 805 32 9
Cuts at: 2913 2945 3734 3766 3950 3982 4172 4204 4839
Size: 32 789 32 184 32 190 32 635
Cuts at: 4839 4841 4871 4873 6628 6660 6702 6734 1373
Size: 2 30 2 1755 32 42 32 2626
Fragments arranged by size:
2626 1755 805 789 635 463 190 184
135 42 32 32 32 32 32 32
32 32 32 32 30 9 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3810 5331 5577 669
Size: 988 281 1872 1521 246 3079
Fragments arranged by size:
3079 1872 1521 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4637 5189 748
Size: 3889 552 3546
Fragments arranged by size:
3889 3546 552
HpaI GTT'AAC
Cuts at: 5577 5577
Size: 7987
KpnI G_GTAC'C
Cuts at: 3323 5312 3323
Size: 1989 5998
MfeI C'AATT_G
Cuts at: 3552 5586 3552
Size: 2034 5953
MluI A'CGCG_T
Cuts at: 7863 7863
Size: 7987
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2479 3561 4158 4215 4583 7101 7285 1305
Size: 1174 1082 597 57 368 2518 184 2007
Fragments arranged by size:
2518 2007 1174 1082 597 368 184 57
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2254 4940 5024 5186 10
Size: 54 1425 343 422 2686 84 162 2811
Fragments arranged by size:
2811 2686 1425 422 343 162 84 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2867 3995 4091 5066 5945 6304 6463
Size: 648 1701 1128 96 975 879 359 159
Cuts at: 6463 518
Size: 2042
Fragments arranged by size:
2042 1701 1128 975 879 648 359 159
96
MspA1I CmG'CkG
Cuts at: 1606 2031 2598 2926 3008 3940 5403 6080 7228
Size: 425 567 328 82 932 1463 677 1148
Cuts at: 7228 7473 1606
Size: 245 2120
Fragments arranged by size:
2120 1463 1148 932 677 567 425 328
245 82
NcoI C'CATG_G
Cuts at: 513 1065 2004 4092 4255 513
Size: 552 939 2088 163 4245
Fragments arranged by size:
4245 2088 939 552 163
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7987
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7987
NotI GC'GGCC_GC
Cuts at: 5434 5434
Size: 7987
NsiI A_TGCA'T
Cuts at: 3365 3365
Size: 7987
NspI r_CATG'y
Cuts at: 1132 1229 2049 2791 4090 5069 5345 6890 1132
Size: 97 820 742 1299 979 276 1545 2229
Fragments arranged by size:
2229 1545 1299 979 820 742 276 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7987
PciI A'CATG_T
Cuts at: 5065 6886 5065
Size: 1821 6166
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3139 4966 1108
Size: 2031 1827 4129
Fragments arranged by size:
4129 2031 1827
PfoI T'CCnGG_A
Cuts at: 2820 2820
Size: 7987
PmeI GTTT'AAAC
Cuts at: 5291 5291
Size: 7987
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4110 4142 6056 6088 7569 7601 4110
Size: 32 1914 32 1481 32 4496
Fragments arranged by size:
4496 1914 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7987
PsiI TTA'TAA
Cuts at: 3209 5557 3209
Size: 2348 5639
PspOMI G'GGCC_C
Cuts at: 2892 2892
Size: 7987
PstI C_TGCA'G
Cuts at: 830 2734 3939 4434 5339 830
Size: 1904 1205 495 905 3478
Fragments arranged by size:
3478 1904 1205 905 495
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7987
PvuII CAG'CTG
Cuts at: 2598 2926 3008 3940 2598
Size: 328 82 932 6645
Fragments arranged by size:
6645 932 328 82
SalI G'TCGA_C
Cuts at: 1936 5329 1936
Size: 3393 4594
SapI GCTCTTCn'nnn_
Cuts at: 4329 5208 4329
Size: 879 7108
SbfI CC_TGCA'GG
Cuts at: 5339 5339
Size: 7987
ScaI AGT'ACT
Cuts at: 6235 7947 6235
Size: 1712 6275
SfcI C'TryA_G
Cuts at: 826 1045 2117 2730 3115 3935 4430 5335 6470
Size: 219 1072 613 385 820 495 905 1135
Cuts at: 6470 7151 7342 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1072 905 820 681 613 495
385 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2018 2018
Size: 7987
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7987
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7987
SmaI CCC'GGG
Cuts at: 1872 2094 5314 1872
Size: 222 3220 4545
Fragments arranged by size:
4545 3220 222
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2107 2779 3633 4455 4622 4643
Size: 197 926 164 672 854 822 167 21
Cuts at: 4643 5129 6091 6992 7254 820
Size: 486 962 901 262 1553
Fragments arranged by size:
1553 962 926 901 854 822 672 486
262 197 167 164 21
SnaBI TAC'GTA
Cuts at: 493 5778 493
Size: 5285 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7987
SphI G_CATG'C
Cuts at: 1132 5345 1132
Size: 4213 3774
SspI AAT'ATT
Cuts at: 5 52 5911 5
Size: 47 5859 2081
Fragments arranged by size:
5859 2081 47
StuI AGG'CCT
Cuts at: 1517 4428 1517
Size: 2911 5076
StyI C'CwwG_G
Cuts at: 513 1065 2004 2372 2466 4092 4255 4945 5426
Size: 552 939 368 94 1626 163 690 481
Cuts at: 5426 513
Size: 3074
Fragments arranged by size:
3074 1626 939 690 552 481 368 163
94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6014 6031 6184 6369 6814 325
Size: 1255 4434 17 153 185 445 1498
Fragments arranged by size:
4434 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2589 4298 6233
Size: 274 80 33 51 1443 612 1709 1935
Cuts at: 6233 7945 96
Size: 1712 138
Fragments arranged by size:
1935 1712 1709 1443 612 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2186 2218 3037 3069 7947 7979 2186
Size: 32 819 32 4878 32 2194
Fragments arranged by size:
4878 2194 819 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4853 1316
Size: 3537 4450
XbaI T'CTAG_A
Cuts at: 5323 5323
Size: 7987
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4099 4099
Size: 7987
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7987
XmaI C'CCGG_G
Cuts at: 1870 2092 5312 1870
Size: 222 3220 4545
Fragments arranged by size:
4545 3220 222
XmnI GAAnn'nnTTC
Cuts at: 2739 5237 6116 2739
Size: 2498 879 4610
Fragments arranged by size:
4610 2498 879
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4726 5795 276
Size: 53 83 186 936 3192 1069 2468
Fragments arranged by size:
3192 2468 1069 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BsiWI BsmBI BstZ17I EcoICRI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfoI SrfI SwaI