Restriction map for
pFN21AA1745
(Circular) (Six-base) MAPSORT of: pFN21AA1745.seq Check: 3686 from: 1 to: 7216
pFN21AA1745 7216 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5026 278
Size: 53 83 186 936 3490 2468
Fragments arranged by size:
3490 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4559 1937
Size: 2622 4594
Acc65I G'GTAC_C
Cuts at: 4362 4537 4362
Size: 175 7041
AclI AA'CG_TT
Cuts at: 1563 5343 5716 1563
Size: 3780 373 3063
Fragments arranged by size:
3780 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2187 2449 2779 3097 4268 5279 6663 6931
Size: 555 262 330 318 1171 1011 1384 268
Cuts at: 6931 1632
Size: 1917
Fragments arranged by size:
1917 1384 1171 1011 555 330 318 268
262
AfeI AGC'GCT
Cuts at: 2900 2900
Size: 7216
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7019
AflIII A'CryG_T
Cuts at: 1203 6115 7092 1203
Size: 4912 977 1327
Fragments arranged by size:
4912 1327 977
AgeI A'CCGG_T
Cuts at: 6047 6047
Size: 7216
AhdI GACnn_n'nnGTC
Cuts at: 5945 5945
Size: 7216
AleI CACnn'nnGTG
Cuts at: 1166 2837 3151 1166
Size: 1671 314 5231
Fragments arranged by size:
5231 1671 314
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3335 1184
Size: 2151 5065
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2804 2836 2804
Size: 32 7184
AlwNI CAG_nnn'CTG
Cuts at: 2326 2929 3505 3597 4000 4014 4372 4959 6531
Size: 603 576 92 403 14 358 587 1572
Cuts at: 6531 2326
Size: 3011
Fragments arranged by size:
3011 1572 603 587 576 403 358 92
14
ApaI G_GGCC'C
Cuts at: 3446 3886 4335 3446
Size: 440 449 6327
Fragments arranged by size:
6327 449 440
ApaLI G'TGCA_C
Cuts at: 3452 4101 4168 4271 5273 6429 3452
Size: 649 67 103 1002 1156 4239
Fragments arranged by size:
4239 1156 1002 649 103 67
ApoI r'AATT_y
Cuts at: 1493 2572 2813 4525 4751 4903 7012 1493
Size: 1079 241 1712 226 152 2109 1697
Fragments arranged by size:
2109 1712 1697 1079 241 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5770 160
Size: 872 4738 1606
Fragments arranged by size:
4738 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7216
AvaI C'yCGr_G
Cuts at: 1870 1943 2262 3216 3447 3550 3618 4387 4395
Size: 73 319 954 231 103 68 769 8
Cuts at: 4395 4476 4541 1870
Size: 81 65 4545
Fragments arranged by size:
4545 954 769 319 231 103 81 73
68 65 8
AvrII C'CTAG_G
Cuts at: 4327 4327
Size: 7216
BamHI G'GATC_C
Cuts at: 3699 4546 4913 3699
Size: 847 367 6002
Fragments arranged by size:
6002 847 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3175 3565 3944 4221 4362
Size: 325 217 627 1388 390 379 277 141
Cuts at: 4362 4537 5992 618
Size: 175 1455 1842
Fragments arranged by size:
1842 1455 1388 627 390 379 325 277
217 175 141
BanII G_rGCy'C
Cuts at: 1391 2165 2610 2696 2993 3446 3886 4335 4535
Size: 774 445 86 297 453 440 449 200
Cuts at: 4535 1391
Size: 4072
Fragments arranged by size:
4072 774 453 449 445 440 297 200
86
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2971 3025 3076 3103 3738 4234 4393
Size: 971 1072 54 51 27 635 496 159
Cuts at: 4393 928
Size: 3751
Fragments arranged by size:
3751 1072 971 635 496 159 54 51
27
BbvCI CC'TCA_GC
Cuts at: 2294 2731 3678 2294
Size: 437 947 5832
Fragments arranged by size:
5832 947 437
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4330 4364 5407 5441 1903
Size: 34 50 34 2309 34 1043 34 3678
Fragments arranged by size:
3678 2309 1043 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4465 5108 6318 4465
Size: 643 1210 5363
Fragments arranged by size:
5363 1210 643
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7216
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2068 5827 136
Size: 107 122 71 631 605 396 3759 1525
Fragments arranged by size:
3759 1525 631 605 396 122 107 71
BglII A'GATC_T
Cuts at: 2781 3474 7211 2781
Size: 693 3737 2786
Fragments arranged by size:
3737 2786 693
BlpI GC'TnA_GC
Cuts at: 2135 2228 2426 2987 4633 2135
Size: 93 198 561 1646 4718
Fragments arranged by size:
4718 1646 561 198 93
Bme1580I G_kGCm'C
Cuts at: 1790 3180 3217 3407 3446 3456 3570 3886 3947
Size: 1390 37 190 39 10 114 316 61
Cuts at: 3947 4105 4172 4275 4335 4469 5277 6433 1790
Size: 158 67 103 60 134 808 1156 2573
Fragments arranged by size:
2573 1390 1156 808 316 190 158 134
114 103 67 61 60 39 37 10
BmgBI CAC'GTC
Cuts at: 1318 3661 1318
Size: 2343 4873
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3037 3933 4193
Size: 416 53 83 399 24 1620 896 260
Cuts at: 4193 4454 4947 5905 442
Size: 261 493 958 1753
Fragments arranged by size:
1753 1620 958 896 493 416 399 261
260 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7216
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2288 2288
Size: 7216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3213 3631 5876 1239
Size: 1974 418 2245 2579
Fragments arranged by size:
2579 2245 1974 418
Bpu10I CC'TnA_GC
Cuts at: 2294 2731 3678 2294
Size: 437 947 5832
Fragments arranged by size:
5832 947 437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2900 3257 5341 6206 6504 2900
Size: 357 2084 865 298 3612
Fragments arranged by size:
3612 2084 865 357 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4301 5879 882
Size: 617 2802 1578 2219
Fragments arranged by size:
2802 2219 1578 617
BsaAI yAC'GTr
Cuts at: 493 1204 2254 3913 5007 493
Size: 711 1050 1659 1094 2702
Fragments arranged by size:
2702 1659 1094 1050 711
BsaBI GATnn'nnATC
Cuts at: 7210 7210
Size: 7216
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5023 5405 6040 275
Size: 53 83 186 936 3490 382 635 1451
Fragments arranged by size:
3490 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4224 5649 6047 6321 6468 6879 4224
Size: 1425 398 274 147 411 4561
Fragments arranged by size:
4561 1425 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2871 2901 3953 3983 1178
Size: 30 165 30 1468 30 1052 30 4411
Fragments arranged by size:
4411 1468 1052 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2044 3479 3602 3833 3998 1186
Size: 858 1435 123 231 165 4404
Fragments arranged by size:
4404 1435 858 231 165 123
BseYI C'CCAG_C
Cuts at: 2716 2922 2993 3955 4125 6419 6915 2716
Size: 206 71 962 170 2294 496 3017
Fragments arranged by size:
3017 2294 962 496 206 170 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2358 2383 3122 3359 3518 4152 1163
Size: 573 622 25 739 237 159 634 4227
Fragments arranged by size:
4227 739 634 622 573 237 159 25
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3418 4666 5427 6455 1540
Size: 275 186 1417 1248 761 1028 2301
Fragments arranged by size:
2301 1417 1248 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2263 2610 2696 2936 3456 4105 4172 4275 5277
Size: 347 86 240 520 649 67 103 1002
Cuts at: 5277 5362 6433 2263
Size: 85 1071 3046
Fragments arranged by size:
3046 1071 1002 649 520 347 240 103
86 85 67
BsmI GAATG_Cn'
Cuts at: 4727 4820 4727
Size: 93 7123
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2165 2263 2610 2696 2936 2993 3180
Size: 399 375 98 347 86 240 57 187
Cuts at: 3180 3217 3407 3446 3456 3570 3886 3947 4105
Size: 37 190 39 10 114 316 61 158
Cuts at: 4105 4172 4275 4335 4469 4535 5277 5362 6433
Size: 67 103 60 134 66 742 85 1071
Cuts at: 6433 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 375 347 316 240
190 187 158 134 114 103 98 86
85 67 66 61 60 57 39 37
10
BspEI T'CCGG_A
Cuts at: 6879 6879
Size: 7216
BspHI T'CATG_A
Cuts at: 5105 6835 5105
Size: 1730 5486
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3289 3939 4292 4571 844
Size: 2445 650 353 279 3489
Fragments arranged by size:
3489 2445 650 353 279
BsrBI CCG'CTC
Cuts at: 1394 1823 2173 2425 2884 3642 3841 4348 5103
Size: 429 350 252 459 758 199 507 755
Cuts at: 5103 1394
Size: 3507
Fragments arranged by size:
3507 758 755 507 459 429 350 252
199
BsrDI GCAATG_nn'
Cuts at: 65 1248 2847 5711 5885 65
Size: 1183 1599 2864 174 1396
Fragments arranged by size:
2864 1599 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3417 4218 5860 6047 1700
Size: 110 1607 801 1642 187 2869
Fragments arranged by size:
2869 1642 1607 801 187 110
BsrGI T'GTAC_A
Cuts at: 96 3355 3588 96
Size: 3259 233 3724
Fragments arranged by size:
3724 3259 233
BssHII G'CGCG_C
Cuts at: 1925 2055 1925
Size: 130 7086
BssSI C'ACGA_G
Cuts at: 5276 6288 5276
Size: 1012 6204
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3505 794
Size: 2711 4505
BstBI TT'CG_AA
Cuts at: 2190 6020 2190
Size: 3830 3386
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7216
BstXI CCAn_nnnn'nTGG
Cuts at: 2590 2590
Size: 7216
BstYI r'GATC_y
Cuts at: 1972 2412 2781 3474 3699 4497 4546 4913 5298
Size: 440 369 693 225 798 49 367 385
Cuts at: 5298 5315 6767 6853 6865 7211 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 798 693 440 385 369 367
346 225 86 49 17 12
Bsu36I CC'TnA_GG
Cuts at: 2027 2027
Size: 7216
BtgI C'CryG_G
Cuts at: 513 1065 2048 3570 3695 513
Size: 552 983 1522 125 4034
Fragments arranged by size:
4034 1522 983 552 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2033 2784 2883 4074 487
Size: 683 56 807 751 99 1191 3629
Fragments arranged by size:
3629 1191 807 751 683 99 56
BtsI GCAGTG_nn'
Cuts at: 2366 2583 3158 3438 4072 4732 5526 5546 2366
Size: 217 575 280 634 660 794 20 4036
Fragments arranged by size:
4036 794 660 634 575 280 217 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7216
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7181
DraI TTT'AAA
Cuts at: 4520 4520
Size: 7216
DraIII CAC_nnn'GTG
Cuts at: 3175 3175
Size: 7216
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3299 6223 809
Size: 507 1983 2924 1802
Fragments arranged by size:
2924 1983 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2069 2174 2649
Size: 54 1425 50 275 18 239 105 475
Cuts at: 2649 2806 3415 3573 3684 4185 4663 5552 7181
Size: 157 609 158 111 501 478 889 1629
Cuts at: 7181 8
Size: 43
Fragments arranged by size:
1629 1425 889 609 501 478 475 275
239 158 157 111 105 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3415 4663 1537
Size: 275 1603 1248 4090
Fragments arranged by size:
4090 1603 1248 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2163 3401 4412 5146 1353
Size: 810 1238 1011 734 3423
Fragments arranged by size:
3423 1238 1011 810 734
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4472 5803 6177 6323 387
Size: 1064 138 2883 1331 374 146 1280
Fragments arranged by size:
2883 1331 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2608 2694 2608
Size: 86 7130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2187 2449 2779 3097 3213 3631 4268
Size: 393 555 262 330 318 116 418 637
Cuts at: 4268 5279 5876 6663 6931 1239
Size: 1011 597 787 268 1524
Fragments arranged by size:
1524 1011 787 637 597 555 418 393
330 318 268 262 116
EcoNI CCTnn'n_nnAGG
Cuts at: 3125 4250 3125
Size: 1125 6091
EcoO109I rG'GnC_Cy
Cuts at: 2244 2269 2380 3443 3622 3730 3882 3883 3928
Size: 25 111 1063 179 108 152 1 45
Cuts at: 3928 4140 4331 4332 4392 2244
Size: 212 191 1 60 5068
Fragments arranged by size:
5068 1063 212 191 179 152 111 108
60 45 25 1 1
EcoRI G'AATT_C
Cuts at: 4525 4903 4525
Size: 378 6838
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2803 3298 2803
Size: 495 6721
FspI TGC'GCA
Cuts at: 2012 5722 2012
Size: 3710 3506
HaeII r_GCGC'y
Cuts at: 1678 2135 2902 3726 6363 1678
Size: 457 767 824 2637 2531
Fragments arranged by size:
2637 2531 824 767 457
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3186 3218 5857 5889 5931
Size: 32 135 32 1614 32 2639 32 42
Cuts at: 5931 5963 1373
Size: 32 2626
Fragments arranged by size:
2639 2626 1614 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4560 4806 669
Size: 988 281 2622 246 3079
Fragments arranged by size:
3079 2622 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7216
HpaI GTT'AAC
Cuts at: 4806 4806
Size: 7216
KpnI G_GTAC'C
Cuts at: 4366 4541 4366
Size: 175 7041
MfeI C'AATT_G
Cuts at: 4815 4815
Size: 7216
MluI A'CGCG_T
Cuts at: 7092 7092
Size: 7216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2147 2232 3873 6330 6514 1305
Size: 842 85 1641 2457 184 2007
Fragments arranged by size:
2457 2007 1641 842 184 85
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2808 3575 3686 10
Size: 54 1425 343 976 767 111 3540
Fragments arranged by size:
3540 1425 976 767 343 111 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2018 2489 2837 3151 5174 5533 5692
Size: 648 852 471 348 314 2023 359 159
Cuts at: 5692 518
Size: 2042
Fragments arranged by size:
2042 2023 852 648 471 359 348 314
159
MspA1I CmG'CkG
Cuts at: 1606 2077 2430 2926 3117 3651 3682 3955 4000
Size: 471 353 496 191 534 31 273 45
Cuts at: 4000 4109 4125 4632 5309 6457 6702 1606
Size: 109 16 507 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 534 507 496 471 353
273 245 191 109 45 31 16
NaeI GCC'GGC
Cuts at: 3419 3419
Size: 7216
NcoI C'CATG_G
Cuts at: 513 1065 2048 3570 513
Size: 552 983 1522 4159
Fragments arranged by size:
4159 1522 983 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7216
NgoMIV G'CCGG_C
Cuts at: 3417 3417
Size: 7216
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7216
NotI GC'GGCC_GC
Cuts at: 4663 4663
Size: 7216
NspI r_CATG'y
Cuts at: 1132 1229 3316 4574 6119 1132
Size: 97 2087 1258 1545 2229
Fragments arranged by size:
2229 2087 1545 1258 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7216
PciI A'CATG_T
Cuts at: 6115 6115
Size: 7216
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3349 3696 3955 4000 4112 1108
Size: 2241 347 259 45 112 4212
Fragments arranged by size:
4212 2241 347 259 112 45
PfoI T'CCnGG_A
Cuts at: 2535 3510 3702 2535
Size: 975 192 6049
Fragments arranged by size:
6049 975 192
PmeI GTTT'AAAC
Cuts at: 4520 4520
Size: 7216
PmlI CAC'GTG
Cuts at: 3913 3913
Size: 7216
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5285 5317 6798 6830 5285
Size: 32 1481 32 5671
Fragments arranged by size:
5671 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2244 2380 3622 3730 3928 4140 4392 2244
Size: 136 1242 108 198 212 252 5068
Fragments arranged by size:
5068 1242 252 212 198 136 108
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7216
PsiI TTA'TAA
Cuts at: 4786 4786
Size: 7216
PspOMI G'GGCC_C
Cuts at: 3442 3882 4331 3442
Size: 440 449 6327
Fragments arranged by size:
6327 449 440
PstI C_TGCA'G
Cuts at: 830 2112 2973 3246 3327 3474 3584 4568 830
Size: 1282 861 273 81 147 110 984 3478
Fragments arranged by size:
3478 1282 984 861 273 147 110 81
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7216
PvuII CAG'CTG
Cuts at: 2926 3651 3682 3955 4000 4109 4125 2926
Size: 725 31 273 45 109 16 6017
Fragments arranged by size:
6017 725 273 109 45 31 16
SacI G_AGCT'C
Cuts at: 2610 2696 2610
Size: 86 7130
SalI G'TCGA_C
Cuts at: 1936 4558 1936
Size: 2622 4594
SanDI GG'GwC_CC
Cuts at: 3622 4392 3622
Size: 770 6446
SapI GCTCTTCn'nnn_
Cuts at: 2163 2163
Size: 7216
SbfI CC_TGCA'GG
Cuts at: 4568 4568
Size: 7216
ScaI AGT'ACT
Cuts at: 5464 7176 5464
Size: 1712 5504
SfcI C'TryA_G
Cuts at: 826 1045 2108 2891 2969 3060 3242 3323 3470
Size: 219 1063 783 78 91 182 81 147
Cuts at: 3470 3580 4564 5699 6380 6571 826
Size: 110 984 1135 681 191 1471
Fragments arranged by size:
1471 1135 1063 984 783 681 219 191
182 147 110 91 81 78
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7216
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7216
SmaI CCC'GGG
Cuts at: 1872 3218 3449 3620 4389 4478 4543 1872
Size: 1346 231 171 769 89 65 4545
Fragments arranged by size:
4545 1346 769 231 171 89 65
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2262 2915 3272 5320 6221 6483
Size: 197 926 319 653 357 2048 901 262
Cuts at: 6483 820
Size: 1553
Fragments arranged by size:
2048 1553 926 901 653 357 319 262
197
SnaBI TAC'GTA
Cuts at: 493 5007 493
Size: 4514 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7216
SphI G_CATG'C
Cuts at: 1132 3316 4574 1132
Size: 2184 1258 3774
Fragments arranged by size:
3774 2184 1258
SspI AAT'ATT
Cuts at: 5 52 5140 5
Size: 47 5088 2081
Fragments arranged by size:
5088 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7216
StyI C'CwwG_G
Cuts at: 513 1065 2048 2635 3570 3726 4327 4486 4655
Size: 552 983 587 935 156 601 159 169
Cuts at: 4655 513
Size: 3074
Fragments arranged by size:
3074 983 935 601 587 552 169 159
156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2246 2702 3533 3962 5243 5260 5413
Size: 1255 666 456 831 429 1281 17 153
Cuts at: 5413 5598 6043 325
Size: 185 445 1498
Fragments arranged by size:
1498 1281 1255 831 666 456 445 429
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3355 3588 5462
Size: 274 80 33 51 1443 1378 233 1874
Cuts at: 5462 7174 96
Size: 1712 138
Fragments arranged by size:
1874 1712 1443 1378 274 233 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2871 2903 3480 3512 3953 3985 4416 4448 7176
Size: 32 577 32 441 32 431 32 2728
Cuts at: 7176 7208 2871
Size: 32 2879
Fragments arranged by size:
2879 2728 577 441 431 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7216
XbaI T'CTAG_A
Cuts at: 4552 4552
Size: 7216
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2590 2590
Size: 7216
XhoI C'TCGA_G
Cuts at: 1943 2262 1943
Size: 319 6897
XmaI C'CCGG_G
Cuts at: 1870 3216 3447 3618 4387 4476 4541 1870
Size: 1346 231 171 769 89 65 4545
Fragments arranged by size:
4545 1346 769 231 171 89 65
XmnI GAAnn'nnTTC
Cuts at: 4154 4241 5345 4154
Size: 87 1104 6025
Fragments arranged by size:
6025 1104 87
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5024 276
Size: 53 83 186 936 3490 2468
Fragments arranged by size:
3490 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI
SwaI