Restriction map for
pFN21AA1741
(Circular) (Six-base) MAPSORT of: pFN21AA1741.seq Check: 4726 from: 1 to: 9892
pFN21AA1741 9892 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4371 6318 6461 4371
Size: 1947 143 7802
Fragments arranged by size:
7802 1947 143
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7702 278
Size: 53 83 186 936 6166 2468
Fragments arranged by size:
6166 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2015 7235 1937
Size: 78 5220 4594
Fragments arranged by size:
5220 4594 78
Acc65I G'GTAC_C
Cuts at: 4625 6779 7213 4625
Size: 2154 434 7304
Fragments arranged by size:
7304 2154 434
AclI AA'CG_TT
Cuts at: 1563 8019 8392 1563
Size: 6456 373 3063
Fragments arranged by size:
6456 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2638 2994 3312 3528 4145 5667 6570 6849
Size: 1006 356 318 216 617 1522 903 279
Cuts at: 6849 6992 7196 7955 9339 9607 1632
Size: 143 204 759 1384 268 1917
Fragments arranged by size:
1917 1522 1384 1006 903 759 617 356
318 279 268 216 204 143
AfeI AGC'GCT
Cuts at: 2468 4870 2468
Size: 2402 7490
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9695
AflIII A'CryG_T
Cuts at: 1203 8791 9768 1203
Size: 7588 977 1327
Fragments arranged by size:
7588 1327 977
AgeI A'CCGG_T
Cuts at: 8723 8723
Size: 9892
AhdI GACnn_n'nnGTC
Cuts at: 4135 8621 4135
Size: 4486 5406
AleI CACnn'nnGTG
Cuts at: 1166 2483 1166
Size: 1317 8575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9892
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4523 4555 4523
Size: 32 9860
AlwNI CAG_nnn'CTG
Cuts at: 2900 2956 3884 3934 4037 5987 6281 6367 6476
Size: 56 928 50 103 1950 294 86 109
Cuts at: 6476 7635 9207 2900
Size: 1159 1572 3585
Fragments arranged by size:
3585 1950 1572 1159 928 294 109 103
86 56 50
ApaI G_GGCC'C
Cuts at: 2143 5216 6760 2143
Size: 3073 1544 5275
Fragments arranged by size:
5275 3073 1544
ApaLI G'TGCA_C
Cuts at: 7949 9105 7949
Size: 1156 8736
ApoI r'AATT_y
Cuts at: 1493 4196 4601 4664 4994 7201 7427 7579 9688
Size: 2703 405 63 330 2207 226 152 2109
Cuts at: 9688 1493
Size: 1697
Fragments arranged by size:
2703 2207 2109 1697 405 330 226 152
63
AseI AT'TA_AT
Cuts at: 160 1032 8446 160
Size: 872 7414 1606
Fragments arranged by size:
7414 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9892
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 2106 2136 2208 3064 3457 3966
Size: 73 112 51 30 72 856 393 509
Cuts at: 3966 4022 4967 5123 6430 6852 7217 1870
Size: 56 945 156 1307 422 365 4545
Fragments arranged by size:
4545 1307 945 856 509 422 393 365
156 112 73 72 56 51 30
BamHI G'GATC_C
Cuts at: 5072 7222 7589 5072
Size: 2150 367 7375
Fragments arranged by size:
7375 2150 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2077 2185 4625 4817 6049
Size: 325 217 627 290 108 2440 192 1232
Cuts at: 6049 6330 6572 6750 6779 6856 6920 7213 8668
Size: 281 242 178 29 77 64 293 1455
Cuts at: 8668 618
Size: 1842
Fragments arranged by size:
2440 1842 1455 1232 627 325 293 290
281 242 217 192 178 108 77 64
29
BanII G_rGCy'C
Cuts at: 1391 2143 3047 3072 3088 3135 3341 3717 3789
Size: 752 904 25 16 47 206 376 72
Cuts at: 3789 4955 5209 5216 5546 6069 6079 6132 6584
Size: 1166 254 7 330 523 10 53 452
Cuts at: 6584 6760 6962 7211 1391
Size: 176 202 249 4072
Fragments arranged by size:
4072 1166 904 752 523 452 376 330
254 249 206 202 176 72 53 47
25 16 10 7
BbeI G_GCGC'C
Cuts at: 6053 6053
Size: 9892
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5028 5468 6246 6567 928
Size: 971 3129 440 778 321 4253
Fragments arranged by size:
4253 3129 971 778 440 321
BbvCI CC'TCA_GC
Cuts at: 3534 5721 6305 6964 7005 3534
Size: 2187 584 659 41 6421
Fragments arranged by size:
6421 2187 659 584 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2766 2800 6789 6823 8083 8117 1903
Size: 34 829 34 3989 34 1260 34 3678
Fragments arranged by size:
3989 3678 1260 829 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7784 8994 7784
Size: 1210 8682
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9892
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2194 3083 3518
Size: 107 122 71 631 605 522 889 435
Cuts at: 3518 3527 5882 6441 6974 7156 8503 136
Size: 9 2355 559 533 182 1347 1525
Fragments arranged by size:
2355 1525 1347 889 631 605 559 533
522 435 182 122 107 71 9
BglII A'GATC_T
Cuts at: 4183 9887 4183
Size: 5704 4188
BlpI GC'TnA_GC
Cuts at: 2032 4119 7000 7309 2032
Size: 2087 2881 309 4615
Fragments arranged by size:
4615 2881 2087 309
Bme1580I G_kGCm'C
Cuts at: 1790 2143 3737 4565 5122 5216 6125 6335 6753
Size: 353 1594 828 557 94 909 210 418
Cuts at: 6753 6760 6923 7953 9109 1790
Size: 7 163 1030 1156 2573
Fragments arranged by size:
2573 1594 1156 1030 909 828 557 418
353 210 163 94 7
BmgBI CAC'GTC
Cuts at: 1318 6565 1318
Size: 5247 4645
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2364 3306 4080
Size: 416 53 83 399 24 947 942 774
Cuts at: 4080 4543 4631 4669 4795 5944 6657 7097 7623
Size: 463 88 38 126 1149 713 440 526
Cuts at: 7623 8581 442
Size: 958 1753
Fragments arranged by size:
1753 1149 958 947 942 774 713 526
463 440 416 399 126 88 83 53
38 24
BmtI G_CTAG'C
Cuts at: 1056 5166 1056
Size: 4110 5782
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3705 4698 6320 7144 3705
Size: 993 1622 824 6453
Fragments arranged by size:
6453 1622 993 824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2352 2673 3072 3287 3433 3869 4014 4134
Size: 1113 321 399 215 146 436 145 120
Cuts at: 4134 4555 4574 4888 5350 5919 6009 6188 6392
Size: 421 19 314 462 569 90 179 204
Cuts at: 6392 6515 6544 8552 1239
Size: 123 29 2008 2579
Fragments arranged by size:
2579 2008 1113 569 462 436 421 399
321 314 215 204 179 146 145 123
120 90 29 19
Bpu10I CC'TnA_GC
Cuts at: 2772 2893 3534 3918 4949 5208 5597 5721 6305
Size: 121 641 384 1031 259 389 124 584
Cuts at: 6305 6956 6964 7005 2772
Size: 651 8 41 5659
Fragments arranged by size:
5659 1031 651 641 584 389 384 259
124 121 41 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2809 3731 3984 5623 8017 8882 9180 2809
Size: 922 253 1639 2394 865 298 3521
Fragments arranged by size:
3521 2394 1639 922 865 298 253
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3968 4206 4293 4313 4936 5127 5507
Size: 617 2469 238 87 20 623 191 380
Cuts at: 5507 6171 6221 8555 882
Size: 664 50 2334 2219
Fragments arranged by size:
2469 2334 2219 664 623 617 380 238
191 87 50 20
BsaAI yAC'GTr
Cuts at: 493 1204 5107 7683 493
Size: 711 3903 2576 2702
Fragments arranged by size:
3903 2702 2576 711
BsaBI GATnn'nnATC
Cuts at: 9886 9886
Size: 9892
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4917 6050 7699 8081
Size: 53 83 186 936 3384 1133 1649 382
Cuts at: 8081 8716 275
Size: 635 1451
Fragments arranged by size:
3384 1649 1451 1133 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 8325 8723 8997 9144 9555 8325
Size: 398 274 147 411 8662
Fragments arranged by size:
8662 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2608 2638 3029 3059 7093
Size: 30 165 30 1205 30 391 30 4034
Cuts at: 7093 7123 1178
Size: 30 3947
Fragments arranged by size:
4034 3947 1205 391 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2084 2349 2378 2393 2741 2746 2830
Size: 895 3 265 29 15 348 5 84
Cuts at: 2830 3245 3248 3779 3959 3962 4311 4415 5377
Size: 415 3 531 180 3 349 104 962
Cuts at: 5377 5751 6238 6389 6467 6500 7022 1186
Size: 374 487 151 78 33 522 4056
Fragments arranged by size:
4056 962 895 531 522 487 415 374
349 348 265 180 151 104 84 78
33 29 15 5 3 3 3
BseYI C'CCAG_C
Cuts at: 2174 2265 2369 2401 2455 3059 3104 3331 3596
Size: 91 104 32 54 604 45 227 265
Cuts at: 3596 3697 4223 4491 4977 5259 5292 5497 6199
Size: 101 526 268 486 282 33 205 702
Cuts at: 6199 9095 9591 2174
Size: 2896 496 2475
Fragments arranged by size:
2896 2475 702 604 526 496 486 282
268 265 227 205 104 101 91 54
45 33 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2433 3045 3653 1163
Size: 573 697 612 608 7402
Fragments arranged by size:
7402 697 612 608 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2545 7342 8103 9131 1540
Size: 275 186 544 4797 761 1028 2301
Fragments arranged by size:
4797 2301 1028 761 544 275 186
BsiHKAI G_wGCw'C
Cuts at: 2712 3303 4364 4458 4955 6069 7953 8038 9109
Size: 591 1061 94 497 1114 1884 85 1071
Cuts at: 9109 2712
Size: 3495
Fragments arranged by size:
3495 1884 1114 1071 1061 591 497 94
85
BsmI GAATG_Cn'
Cuts at: 7403 7496 7403
Size: 93 9799
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2143 2712 3047 3072 3088 3135 3303
Size: 399 353 569 335 25 16 47 168
Cuts at: 3303 3341 3717 3737 3789 4364 4458 4565 4955
Size: 38 376 20 52 575 94 107 390
Cuts at: 4955 5122 5209 5216 5546 6069 6079 6125 6132
Size: 167 87 7 330 523 10 46 7
Cuts at: 6132 6335 6584 6753 6760 6923 6962 7211 7953
Size: 203 249 169 7 163 39 249 742
Cuts at: 7953 8038 9109 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 575 569 523 399 390
376 353 335 330 249 249 203 169
168 167 163 107 94 87 85 52
47 46 39 38 25 20 16 10
7 7 7
BspEI T'CCGG_A
Cuts at: 9555 9555
Size: 9892
BspHI T'CATG_A
Cuts at: 7781 9511 7781
Size: 1730 8162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2803 4157 4371 6318 6461 7247 844
Size: 1959 1354 214 1947 143 786 3489
Fragments arranged by size:
3489 1959 1947 1354 786 214 143
BsrBI CCG'CTC
Cuts at: 1394 1823 7779 1394
Size: 429 5956 3507
Fragments arranged by size:
5956 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 8387 8561 65
Size: 1183 7139 174 1396
Fragments arranged by size:
7139 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5055 5621 8536 8723 1700
Size: 110 3245 566 2915 187 2869
Fragments arranged by size:
3245 2915 2869 566 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9892
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9892
BssSI C'ACGA_G
Cuts at: 3448 7952 8964 3448
Size: 4504 1012 4376
Fragments arranged by size:
4504 4376 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6476 794
Size: 5682 4210
BstBI TT'CG_AA
Cuts at: 3432 7064 8696 3432
Size: 3632 1632 4628
Fragments arranged by size:
4628 3632 1632
BstEII G'GTnAC_C
Cuts at: 1184 5968 1184
Size: 4784 5108
BstXI CCAn_nnnn'nTGG
Cuts at: 2182 2273 2354 2485 3271 5530 6199 6406 6522
Size: 91 81 131 786 2259 669 207 116
Cuts at: 6522 2182
Size: 5552
Fragments arranged by size:
5552 2259 786 669 207 131 116 91
81
BstYI r'GATC_y
Cuts at: 1972 2493 3199 4183 5072 6656 6696 7222 7589
Size: 521 706 984 889 1584 40 526 367
Cuts at: 7589 7974 7991 9443 9529 9541 9887 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1584 1452 984 889 706 526 521
385 367 346 86 40 17 12
Bsu36I CC'TnA_GG
Cuts at: 2724 2887 3120 5954 6137 7129 2724
Size: 163 233 2834 183 992 5487
Fragments arranged by size:
5487 2834 992 233 183 163
BtgI C'CryG_G
Cuts at: 513 1065 2037 2481 4747 5568 6842 7050 513
Size: 552 972 444 2266 821 1274 208 3355
Fragments arranged by size:
3355 2266 1274 972 821 552 444 208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3687 487
Size: 683 56 2461 6692
Fragments arranged by size:
6692 2461 683 56
BtsI GCAGTG_nn'
Cuts at: 2048 2619 3932 4897 5097 5227 7408 8202 8222
Size: 571 1313 965 200 130 2181 794 20
Cuts at: 8222 2048
Size: 3718
Fragments arranged by size:
3718 2181 1313 965 794 571 200 130
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9892
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2373 2408 529
Size: 35 1809 35 8013
Fragments arranged by size:
8013 1809 35 35
DraI TTT'AAA
Cuts at: 7196 7196
Size: 9892
DraIII CAC_nnn'GTG
Cuts at: 4088 5449 4088
Size: 1361 8531
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5782 6910 8899 809
Size: 507 4466 1128 1989 1802
Fragments arranged by size:
4466 1989 1802 1128 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2475 2499 3549
Size: 54 1425 50 275 18 645 24 1050
Cuts at: 3549 4393 5053 5057 7339 8228 9857 8
Size: 844 660 4 2282 889 1629 43
Fragments arranged by size:
2282 1629 1425 1050 889 844 660 645
275 54 50 43 24 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 7339 1537
Size: 275 5527 4090
Fragments arranged by size:
5527 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2484 2657 2700 4667 4730 4793 4859 5643
Size: 1131 173 43 1967 63 63 66 784
Cuts at: 5643 5699 6589 6867 7822 1353
Size: 56 890 278 955 3423
Fragments arranged by size:
3423 1967 1131 955 890 784 278 173
66 63 63 56 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2441 3248 8479 8853 8999 387
Size: 1064 138 852 807 5231 374 146 1280
Fragments arranged by size:
5231 1280 1064 852 807 374 146 138
EcoICRI GAG'CTC
Cuts at: 4953 6067 4953
Size: 1114 8778
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2352 2638 2673 2994 3072 3287 3312
Size: 393 720 286 35 321 78 215 25
Cuts at: 3312 3433 3528 3869 4014 4134 4145 4555 4574
Size: 121 95 341 145 120 11 410 19
Cuts at: 4574 4888 5350 5667 5919 6009 6188 6392 6515
Size: 314 462 317 252 90 179 204 123
Cuts at: 6515 6544 6570 6849 6992 7196 7955 8552 9339
Size: 29 26 279 143 204 759 597 787
Cuts at: 9339 9607 1239
Size: 268 1524
Fragments arranged by size:
1524 787 759 720 597 462 410 393
341 321 317 314 286 279 268 252
215 204 204 179 145 143 123 121
120 95 90 78 35 29 26 25
19 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2771 3179 3863 5033 6371 6449 6523 6794 6824
Size: 408 684 1170 1338 78 74 271 30
Cuts at: 6824 2771
Size: 5839
Fragments arranged by size:
5839 1338 1170 684 408 271 78 74
30
EcoO109I rG'GnC_Cy
Cuts at: 2140 2213 2261 2437 2527 2604 2764 2855 2897
Size: 73 48 176 90 77 160 91 42
Cuts at: 2897 3094 3453 4034 4908 5212 5213 6364 6442
Size: 197 359 581 874 304 1 1151 78
Cuts at: 6442 6644 6757 7078 2140
Size: 202 113 321 4954
Fragments arranged by size:
4954 1151 874 581 359 321 304 202
197 176 160 113 91 90 78 77
73 48 42 1
EcoRI G'AATT_C
Cuts at: 4601 7201 7579 4601
Size: 2600 378 6914
Fragments arranged by size:
6914 2600 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5045 5045
Size: 9892
FseI GG_CCGG'CC
Cuts at: 5059 5059
Size: 9892
FspI TGC'GCA
Cuts at: 8398 8398
Size: 9892
HaeII r_GCGC'y
Cuts at: 1678 2290 2470 3283 4872 6053 9039 1678
Size: 612 180 813 1589 1181 2986 2531
Fragments arranged by size:
2986 2531 1589 1181 813 612 180
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2570 2602 2608 2640 3029
Size: 32 135 32 998 32 6 32 389
Cuts at: 3029 3061 3982 4014 4121 4153 5057 5089 5247
Size: 32 921 32 107 32 904 32 158
Cuts at: 5247 5279 5353 5385 5969 6001 6233 6265 7016
Size: 32 74 32 584 32 232 32 751
Cuts at: 7016 7048 8533 8565 8607 8639 1373
Size: 32 1485 32 42 32 2626
Fragments arranged by size:
2626 1485 998 921 904 751 584 389
232 158 135 107 74 42 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3686 6940 7236 7482 669
Size: 988 281 1748 3254 296 246 3079
Fragments arranged by size:
3254 3079 1748 988 296 281 246
HindIII A'AGCT_T
Cuts at: 748 2976 4930 748
Size: 2228 1954 5710
Fragments arranged by size:
5710 2228 1954
HpaI GTT'AAC
Cuts at: 7482 7482
Size: 9892
KasI G'GCGC_C
Cuts at: 6049 6049
Size: 9892
KpnI G_GTAC'C
Cuts at: 4629 6783 7217 4629
Size: 2154 434 7304
Fragments arranged by size:
7304 2154 434
MfeI C'AATT_G
Cuts at: 7491 7491
Size: 9892
MluI A'CGCG_T
Cuts at: 9768 9768
Size: 9892
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2769 3432 4459 5812 6429 6774 9006 9190
Size: 1464 663 1027 1353 617 345 2232 184
Cuts at: 9190 1305
Size: 2007
Fragments arranged by size:
2232 2007 1464 1353 1027 663 617 345
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2501 10
Size: 54 1425 343 669 7401
Fragments arranged by size:
7401 1425 669 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2483 7850 8209 8368 518
Size: 648 843 474 5367 359 159 2042
Fragments arranged by size:
5367 2042 843 648 474 359 159
MspA1I CmG'CkG
Cuts at: 1606 3002 3701 4053 4245 4507 5145 5471 5497
Size: 1396 699 352 192 262 638 326 26
Cuts at: 5497 5526 6425 7004 7052 7308 7985 9133 9378
Size: 29 899 579 48 256 677 1148 245
Cuts at: 9378 1606
Size: 2120
Fragments arranged by size:
2120 1396 1148 899 699 677 638 579
352 326 262 256 245 192 48 29
26
NaeI GCC'GGC
Cuts at: 5057 5057
Size: 9892
NarI GG'CG_CC
Cuts at: 6050 6050
Size: 9892
NcoI C'CATG_G
Cuts at: 513 1065 2037 4747 513
Size: 552 972 2710 5658
Fragments arranged by size:
5658 2710 972 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9892
NgoMIV G'CCGG_C
Cuts at: 5055 5055
Size: 9892
NheI G'CTAG_C
Cuts at: 1052 5162 1052
Size: 4110 5782
NotI GC'GGCC_GC
Cuts at: 7339 7339
Size: 9892
NruI TCG'CGA
Cuts at: 2590 2590
Size: 9892
NspI r_CATG'y
Cuts at: 1132 1229 2254 3655 7250 8795 1132
Size: 97 1025 1401 3595 1545 2229
Fragments arranged by size:
3595 2229 1545 1401 1025 97
PasI CC'CwG_GG
Cuts at: 1348 2420 2628 4448 4558 5315 5879 6232 6361
Size: 1072 208 1820 110 757 564 353 129
Cuts at: 6361 1348
Size: 4879
Fragments arranged by size:
4879 1820 1072 757 564 353 208 129
110
PciI A'CATG_T
Cuts at: 8791 8791
Size: 9892
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2814 3142 4088 4371 4729 5173 5987 6046
Size: 1706 328 946 283 358 444 814 59
Cuts at: 6046 6061 1108
Size: 15 4939
Fragments arranged by size:
4939 1706 946 814 444 358 328 283
59 15
PfoI T'CCnGG_A
Cuts at: 3846 4590 4904 5896 6521 6825 3846
Size: 744 314 992 625 304 6913
Fragments arranged by size:
6913 992 744 625 314 304
PmeI GTTT'AAAC
Cuts at: 7196 7196
Size: 9892
PmlI CAC'GTG
Cuts at: 5107 5107
Size: 9892
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7961 7993 9474 9506 7961
Size: 32 1481 32 8347
Fragments arranged by size:
8347 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2213 2261 2527 2604 2764 4908 7078 2213
Size: 48 266 77 160 2144 2170 5027
Fragments arranged by size:
5027 2170 2144 266 160 77 48
PshAI GACnn'nnGTC
Cuts at: 1613 3802 4305 1613
Size: 2189 503 7200
Fragments arranged by size:
7200 2189 503
PsiI TTA'TAA
Cuts at: 7462 7462
Size: 9892
PspOMI G'GGCC_C
Cuts at: 2139 5212 6756 2139
Size: 3073 1544 5275
Fragments arranged by size:
5275 3073 1544
PstI C_TGCA'G
Cuts at: 830 4191 5894 6277 7244 830
Size: 3361 1703 383 967 3478
Fragments arranged by size:
3478 3361 1703 967 383
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9892
PvuII CAG'CTG
Cuts at: 3701 4245 5145 5471 5497 5526 7004 3701
Size: 544 900 326 26 29 1478 6589
Fragments arranged by size:
6589 1478 900 544 326 29 26
SacI G_AGCT'C
Cuts at: 4955 6069 4955
Size: 1114 8778
SacII CC_GC'GG
Cuts at: 7053 7053
Size: 9892
SalI G'TCGA_C
Cuts at: 1936 7234 1936
Size: 5298 4594
SanDI GG'GwC_CC
Cuts at: 2213 2213
Size: 9892
SapI GCTCTTCn'nnn_
Cuts at: 2700 4859 5643 5699 6589 2700
Size: 2159 784 56 890 6003
Fragments arranged by size:
6003 2159 890 784 56
SbfI CC_TGCA'GG
Cuts at: 7244 7244
Size: 9892
ScaI AGT'ACT
Cuts at: 8140 9852 8140
Size: 1712 8180
SfcI C'TryA_G
Cuts at: 826 1045 4187 4630 4885 5890 6273 6834 7240
Size: 219 3142 443 255 1005 383 561 406
Cuts at: 7240 8375 9056 9247 826
Size: 1135 681 191 1471
Fragments arranged by size:
3142 1471 1135 1005 681 561 443 406
383 255 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 6441 6441
Size: 9892
SfoI GGC'GCC
Cuts at: 6051 6051
Size: 9892
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9892
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9892
SmaI CCC'GGG
Cuts at: 1872 2057 2138 2210 3066 3968 7219 1872
Size: 185 81 72 856 902 3251 4545
Fragments arranged by size:
4545 3251 902 856 185 81 72
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2824 3710 3999 5602 7996 8897
Size: 197 926 881 886 289 1603 2394 901
Cuts at: 8897 9159 820
Size: 262 1553
Fragments arranged by size:
2394 1603 1553 926 901 886 881 289
262 197
SnaBI TAC'GTA
Cuts at: 493 7683 493
Size: 7190 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9892
SphI G_CATG'C
Cuts at: 1132 7250 1132
Size: 6118 3774
SrfI GCCC'GGGC
Cuts at: 3968 3968
Size: 9892
SspI AAT'ATT
Cuts at: 5 52 7816 5
Size: 47 7764 2081
Fragments arranged by size:
7764 2081 47
StuI AGG'CCT
Cuts at: 1517 2311 2324 2871 1517
Size: 794 13 547 8538
Fragments arranged by size:
8538 794 547 13
StyI C'CwwG_G
Cuts at: 513 1065 2037 2306 3240 3358 3789 4165 4276
Size: 552 972 269 934 118 431 376 111
Cuts at: 4276 4651 4747 5029 5857 6183 6258 6399 6436
Size: 375 96 282 828 326 75 141 37
Cuts at: 6436 6640 6783 6924 7331 513
Size: 204 143 141 407 3074
Fragments arranged by size:
3074 972 934 828 552 431 407 376
375 326 282 269 204 143 141 141
118 111 96 75 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2360 3176 3325 3619 3677 3995 4099
Size: 1255 780 816 149 294 58 318 104
Cuts at: 4099 4676 7919 7936 8089 8274 8719 325
Size: 577 3243 17 153 185 445 1498
Fragments arranged by size:
3243 1498 1255 816 780 577 445 318
294 185 153 149 104 58 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 8138 9850 96
Size: 274 80 33 51 1443 6161 1712 138
Fragments arranged by size:
6161 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6003 6035 6807 6839 7093 7125 9852 9884 6003
Size: 32 772 32 254 32 2727 32 6011
Fragments arranged by size:
6011 2727 772 254 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9892
XbaI T'CTAG_A
Cuts at: 7228 7228
Size: 9892
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2273 3671 5111 6181 2273
Size: 1398 1440 1070 5984
Fragments arranged by size:
5984 1440 1398 1070
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9892
XmaI C'CCGG_G
Cuts at: 1870 2055 2136 2208 3064 3966 7217 1870
Size: 185 81 72 856 902 3251 4545
Fragments arranged by size:
4545 3251 902 856 185 81 72
XmnI GAAnn'nnTTC
Cuts at: 8021 8021
Size: 9892
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7700 276
Size: 53 83 186 936 6166 2468
Fragments arranged by size:
6166 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV
FspAI NsiI PacI PspXI PsrI RsrII SexAI SwaI