Restriction map for
pFN21AA1738
(Circular) (Six-base) MAPSORT of: pFN21AA1738.seq Check: 5977 from: 1 to: 8086
pFN21AA1738 8086 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4338 4338
Size: 8086
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5896 278
Size: 53 83 186 936 4360 2468
Fragments arranged by size:
4360 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2591 5429 1937
Size: 654 2838 4594
Fragments arranged by size:
4594 2838 654
Acc65I G'GTAC_C
Cuts at: 5407 5407
Size: 8086
AclI AA'CG_TT
Cuts at: 1563 6213 6586 1563
Size: 4650 373 3063
Fragments arranged by size:
4650 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2964 3194 3209 3665 3954 4802 4950 6149
Size: 1332 230 15 456 289 848 148 1199
Cuts at: 6149 7533 7801 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1332 1199 848 456 289 268
230 148 15
AfeI AGC'GCT
Cuts at: 4861 4861
Size: 8086
AflII C'TTAA_G
Cuts at: 820 1017 3739 820
Size: 197 2722 5167
Fragments arranged by size:
5167 2722 197
AflIII A'CryG_T
Cuts at: 1203 5167 6985 7962 1203
Size: 3964 1818 977 1327
Fragments arranged by size:
3964 1818 1327 977
AgeI A'CCGG_T
Cuts at: 6917 6917
Size: 8086
AhdI GACnn_n'nnGTC
Cuts at: 3940 6815 3940
Size: 2875 5211
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8086
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8086
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2141 2173 2141
Size: 32 8054
AlwNI CAG_nnn'CTG
Cuts at: 4928 5829 7401 4928
Size: 901 1572 5613
Fragments arranged by size:
5613 1572 901
ApaI G_GGCC'C
Cuts at: 2433 2433
Size: 8086
ApaLI G'TGCA_C
Cuts at: 2072 6143 7299 2072
Size: 4071 1156 2859
Fragments arranged by size:
4071 2859 1156
ApoI r'AATT_y
Cuts at: 1493 4741 5395 5621 5773 7882 1493
Size: 3248 654 226 152 2109 1697
Fragments arranged by size:
3248 2109 1697 654 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6640 160
Size: 872 5608 1606
Fragments arranged by size:
5608 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8086
AvaI C'yCGr_G
Cuts at: 1870 1943 2094 4727 4818 5411 1870
Size: 73 151 2633 91 593 4545
Fragments arranged by size:
4545 2633 593 151 91 73
BamHI G'GATC_C
Cuts at: 5416 5783 5416
Size: 367 7719
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4221 5407 6862 618
Size: 325 217 627 2434 1186 1455 1842
Fragments arranged by size:
2434 1842 1455 1186 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2433 3132 4388 4599 4896 5405 1391
Size: 1042 699 1256 211 297 509 4072
Fragments arranged by size:
4072 1256 1042 699 509 297 211
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2229 2412 2654 3197 3431 4884 928
Size: 971 330 183 242 543 234 1453 4130
Fragments arranged by size:
4130 1453 971 543 330 242 234 183
BbvCI CC'TCA_GC
Cuts at: 3990 3990
Size: 8086
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6277 6311 1903
Size: 34 4340 34 3678
Fragments arranged by size:
4340 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2689 5978 7188 2689
Size: 3289 1210 3587
Fragments arranged by size:
3587 3289 1210
BclI T'GATC_A
Cuts at: 1548 4666 5377 1548
Size: 3118 711 4257
Fragments arranged by size:
4257 3118 711
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6697 136
Size: 107 122 71 631 605 5025 1525
Fragments arranged by size:
5025 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3614 4040 8081 3614
Size: 426 4041 3619
Fragments arranged by size:
4041 3619 426
BlpI GC'TnA_GC
Cuts at: 5503 5503
Size: 8086
Bme1580I G_kGCm'C
Cuts at: 1790 2076 2433 2847 3015 4224 6147 7303 1790
Size: 286 357 414 168 1209 1923 1156 2573
Fragments arranged by size:
2573 1923 1209 1156 414 357 286 168
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8086
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2442 5817 6775
Size: 416 53 83 399 24 1025 3375 958
Cuts at: 6775 442
Size: 1753
Fragments arranged by size:
3375 1753 1025 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2442 1056
Size: 1386 6700
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3409 4870 3409
Size: 1461 6625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3230 4188 4628 4840 5270 6746 1239
Size: 1991 958 440 212 430 1476 2579
Fragments arranged by size:
2579 1991 1476 958 440 430 212
Bpu10I CC'TnA_GC
Cuts at: 2434 3990 2434
Size: 1556 6530
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3320 3746 6211 7076 7374 3320
Size: 426 2465 865 298 4032
Fragments arranged by size:
4032 2465 865 426 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4724 6749 882
Size: 617 3225 2025 2219
Fragments arranged by size:
3225 2219 2025 617
BsaAI yAC'GTr
Cuts at: 493 1204 2077 5877 493
Size: 711 873 3800 2702
Fragments arranged by size:
3800 2702 873 711
BsaBI GATnn'nnATC
Cuts at: 8080 8080
Size: 8086
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2881 5893 6275 6910
Size: 53 83 186 936 1348 3012 382 635
Cuts at: 6910 275
Size: 1451
Fragments arranged by size:
3012 1451 1348 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6519 6917 7191 7338 7749 6519
Size: 398 274 147 411 6856
Fragments arranged by size:
6856 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2141 2171 3038 3068 4256
Size: 30 165 30 738 30 867 30 1188
Cuts at: 4256 4286 4902 4932 5243 5273 1178
Size: 30 616 30 311 30 3991
Fragments arranged by size:
3991 1188 867 738 616 311 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 2150 2153 2162 2165 2168 2189 3062
Size: 961 3 3 9 3 3 21 873
Cuts at: 3062 3065 4277 4396 4466 4858 4907 1186
Size: 3 1212 119 70 392 49 4365
Fragments arranged by size:
4365 1212 961 873 392 119 70 49
21 9 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 4418 4895 7289 7785 4418
Size: 477 2394 496 4719
Fragments arranged by size:
4719 2394 496 477
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2099 3422 4176 1163
Size: 573 363 1323 754 5073
Fragments arranged by size:
5073 1323 754 573 363
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2595 3399 5536 6297 7325 1540
Size: 275 186 594 804 2137 761 1028 2301
Fragments arranged by size:
2301 2137 1028 804 761 594 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 3351 3568 4599 6147 6232 7303 2076
Size: 1275 217 1031 1548 85 1071 2859
Fragments arranged by size:
2859 1548 1275 1071 1031 217 85
BsmI GAATG_Cn'
Cuts at: 4483 4823 5597 5690 4483
Size: 340 774 93 6879
Fragments arranged by size:
6879 774 340 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2335 2335
Size: 8086
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2433 2847 3015 3132 3351 3568
Size: 399 286 357 414 168 117 219 217
Cuts at: 3568 4224 4388 4599 4896 5405 6147 6232 7303
Size: 656 164 211 297 509 742 85 1071
Cuts at: 7303 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 656 509 414 399 357
297 286 219 217 211 168 164 117
85
BspEI T'CCGG_A
Cuts at: 7749 7749
Size: 8086
BspHI T'CATG_A
Cuts at: 2568 3522 5975 7705 2568
Size: 954 2453 1730 2949
Fragments arranged by size:
2949 2453 1730 954
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4338 5441 844
Size: 3494 1103 3489
Fragments arranged by size:
3494 3489 1103
BsrBI CCG'CTC
Cuts at: 1394 1823 5973 1394
Size: 429 4150 3507
Fragments arranged by size:
4150 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6581 6755 65
Size: 1183 5333 174 1396
Fragments arranged by size:
5333 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2261 6730 6917 1700
Size: 110 451 4469 187 2869
Fragments arranged by size:
4469 2869 451 187 110
BsrGI T'GTAC_A
Cuts at: 96 3267 4338 96
Size: 3171 1071 3844
Fragments arranged by size:
3844 3171 1071
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8086
BssSI C'ACGA_G
Cuts at: 6146 7158 6146
Size: 1012 7074
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4318 794
Size: 3524 4562
BstBI TT'CG_AA
Cuts at: 6890 6890
Size: 8086
BstEII G'GTnAC_C
Cuts at: 1184 3805 1184
Size: 2621 5465
BstYI r'GATC_y
Cuts at: 1972 3614 4040 4548 5416 5783 6168 6185 7637
Size: 1642 426 508 868 367 385 17 1452
Cuts at: 7637 7723 7735 8081 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1642 1452 868 508 426 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4026 4026
Size: 8086
BtgI C'CryG_G
Cuts at: 513 1065 2670 5322 513
Size: 552 1605 2652 3277
Fragments arranged by size:
3277 2652 1605 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2089 4857 487
Size: 683 56 863 2768 3716
Fragments arranged by size:
3716 2768 863 683 56
BtsI GCAGTG_nn'
Cuts at: 4261 5602 6396 6416 4261
Size: 1341 794 20 5931
Fragments arranged by size:
5931 1341 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8086
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8051
DraI TTT'AAA
Cuts at: 2839 5390 2839
Size: 2551 5535
DraIII CAC_nnn'GTG
Cuts at: 3487 3563 3487
Size: 76 8010
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4209 7093 809
Size: 507 2893 2884 1802
Fragments arranged by size:
2893 2884 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2248 3396 4084
Size: 54 1425 50 275 18 418 1148 688
Cuts at: 4084 5186 5533 6422 8051 8
Size: 1102 347 889 1629 43
Fragments arranged by size:
1629 1425 1148 1102 889 688 418 347
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3396 5533 1537
Size: 275 1584 2137 4090
Fragments arranged by size:
4090 2137 1584 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2139 3246 3969 6016 1353
Size: 786 1107 723 2047 3423
Fragments arranged by size:
3423 2047 1107 786 723
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4850 6673 7047 7193 387
Size: 1064 138 3261 1823 374 146 1280
Fragments arranged by size:
3261 1823 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4597 4597
Size: 8086
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2964 3194 3209 3230 3665 3954 4188
Size: 393 1332 230 15 21 435 289 234
Cuts at: 4188 4628 4802 4840 4950 5270 6149 6746 7533
Size: 440 174 38 110 320 879 597 787
Cuts at: 7533 7801 1239
Size: 268 1524
Fragments arranged by size:
1524 1332 879 787 597 440 435 393
320 289 268 234 230 174 110 38
21 15
EcoO109I rG'GnC_Cy
Cuts at: 2311 2429 2430 4003 4710 4815 2311
Size: 118 1 1573 707 105 5582
Fragments arranged by size:
5582 1573 707 118 105 1
EcoRI G'AATT_C
Cuts at: 5395 5773 5395
Size: 378 7708
EcoRV GAT'ATC
Cuts at: 3625 3625
Size: 8086
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4784 4784
Size: 8086
FspI TGC'GCA
Cuts at: 6592 6592
Size: 8086
HaeII r_GCGC'y
Cuts at: 1678 4863 7233 1678
Size: 3185 2370 2531
Fragments arranged by size:
3185 2531 2370
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2088 2120 2282 2314 2687
Size: 32 135 32 516 32 162 32 373
Cuts at: 2687 2719 3184 3216 3305 3337 3926 3958 4051
Size: 32 465 32 89 32 589 32 93
Cuts at: 4051 4083 4256 4288 4523 4555 4835 4867 5318
Size: 32 173 32 235 32 280 32 451
Cuts at: 5318 5350 6727 6759 6801 6833 1373
Size: 32 1377 32 42 32 2626
Fragments arranged by size:
2626 1377 589 516 465 451 373 280
235 173 162 135 93 89 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2592 5113 5430 5676 669
Size: 988 281 654 2521 317 246 3079
Fragments arranged by size:
3079 2521 988 654 317 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8086
HpaI GTT'AAC
Cuts at: 5676 5676
Size: 8086
KpnI G_GTAC'C
Cuts at: 5411 5411
Size: 8086
MfeI C'AATT_G
Cuts at: 5685 5685
Size: 8086
MluI A'CGCG_T
Cuts at: 7962 7962
Size: 8086
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2334 2673 3114 4119 4768 4921 5079 7200
Size: 1029 339 441 1005 649 153 158 2121
Cuts at: 7200 7384 1305
Size: 184 2007
Fragments arranged by size:
2121 2007 1029 1005 649 441 339 184
158 153
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2250 10
Size: 54 1425 343 418 5846
Fragments arranged by size:
5846 1425 418 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 4622 5309 6044 6403 6562 518
Size: 648 879 2577 687 735 359 159 2042
Fragments arranged by size:
2577 2042 879 735 687 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3989 4418 4899 5502 6179 7327 7572 1606
Size: 2383 429 481 603 677 1148 245 2120
Fragments arranged by size:
2383 2120 1148 677 603 481 429 245
NaeI GCC'GGC
Cuts at: 2263 2263
Size: 8086
NcoI C'CATG_G
Cuts at: 513 1065 5322 513
Size: 552 4257 3277
Fragments arranged by size:
4257 3277 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8086
NgoMIV G'CCGG_C
Cuts at: 2261 2261
Size: 8086
NheI G'CTAG_C
Cuts at: 1052 2438 1052
Size: 1386 6700
NotI GC'GGCC_GC
Cuts at: 5533 5533
Size: 8086
NspI r_CATG'y
Cuts at: 1132 1229 2050 5171 5444 6989 1132
Size: 97 821 3121 273 1545 2229
Fragments arranged by size:
3121 2229 1545 821 273 97
PasI CC'CwG_GG
Cuts at: 1348 2411 3160 4922 1348
Size: 1063 749 1762 4512
Fragments arranged by size:
4512 1762 1063 749
PciI A'CATG_T
Cuts at: 5167 6985 5167
Size: 1818 6268
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5195 1108
Size: 4087 3999
PfoI T'CCnGG_A
Cuts at: 3770 3770
Size: 8086
PmeI GTTT'AAAC
Cuts at: 5390 5390
Size: 8086
PmlI CAC'GTG
Cuts at: 2077 2077
Size: 8086
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2141 2173 6155 6187 7668 7700 2141
Size: 32 3982 32 1481 32 2527
Fragments arranged by size:
3982 2527 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4003 4710 4003
Size: 707 7379
PshAI GACnn'nnGTC
Cuts at: 1613 2654 1613
Size: 1041 7045
PsiI TTA'TAA
Cuts at: 3443 5656 3443
Size: 2213 5873
PspOMI G'GGCC_C
Cuts at: 2429 2429
Size: 8086
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3624 3656 3624
Size: 32 8054
PstI C_TGCA'G
Cuts at: 830 3532 4395 5438 830
Size: 2702 863 1043 3478
Fragments arranged by size:
3478 2702 1043 863
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8086
PvuII CAG'CTG
Cuts at: 3989 4418 3989
Size: 429 7657
SacI G_AGCT'C
Cuts at: 4599 4599
Size: 8086
SalI G'TCGA_C
Cuts at: 1936 2590 5428 1936
Size: 654 2838 4594
Fragments arranged by size:
4594 2838 654
SbfI CC_TGCA'GG
Cuts at: 5438 5438
Size: 8086
ScaI AGT'ACT
Cuts at: 2168 6334 8046 2168
Size: 4166 1712 2208
Fragments arranged by size:
4166 2208 1712
SfcI C'TryA_G
Cuts at: 826 1045 3034 3528 4330 4391 5434 6569 7250
Size: 219 1989 494 802 61 1043 1135 681
Cuts at: 7250 7441 826
Size: 191 1471
Fragments arranged by size:
1989 1471 1135 1043 802 681 494 219
191 61
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8086
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8086
SmaI CCC'GGG
Cuts at: 1872 4820 5413 1872
Size: 2948 593 4545
Fragments arranged by size:
4545 2948 593
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3335 3725 3739 4727 6190 7091
Size: 197 926 1392 390 14 988 1463 901
Cuts at: 7091 7353 820
Size: 262 1553
Fragments arranged by size:
1553 1463 1392 988 926 901 390 262
197 14
SnaBI TAC'GTA
Cuts at: 493 5877 493
Size: 5384 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8086
SphI G_CATG'C
Cuts at: 1132 5444 1132
Size: 4312 3774
SspI AAT'ATT
Cuts at: 5 52 4497 5000 6010 5
Size: 47 4445 503 1010 2081
Fragments arranged by size:
4445 2081 1010 503 47
StuI AGG'CCT
Cuts at: 1517 3186 4649 1517
Size: 1669 1463 4954
Fragments arranged by size:
4954 1669 1463
StyI C'CwwG_G
Cuts at: 513 1065 2217 3514 4672 5103 5322 5525 513
Size: 552 1152 1297 1158 431 219 203 3074
Fragments arranged by size:
3074 1297 1158 1152 552 431 219 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2609 3126 4462 5151 6113 6130 6283
Size: 1255 1029 517 1336 689 962 17 153
Cuts at: 6283 6468 6913 325
Size: 185 445 1498
Fragments arranged by size:
1498 1336 1255 1029 962 689 517 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2166 2387 3267
Size: 274 80 33 51 1443 189 221 880
Cuts at: 3267 4338 5083 6332 8044 96
Size: 1071 745 1249 1712 138
Fragments arranged by size:
1712 1443 1249 1071 880 745 274 221
189 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2286 2318 3038 3070 3101 3133 8046 8078 2286
Size: 32 720 32 31 32 4913 32 2294
Fragments arranged by size:
4913 2294 720 32 32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8086
XbaI T'CTAG_A
Cuts at: 5422 5422
Size: 8086
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3488 3488
Size: 8086
XhoI C'TCGA_G
Cuts at: 1943 4727 1943
Size: 2784 5302
XmaI C'CCGG_G
Cuts at: 1870 4818 5411 1870
Size: 2948 593 4545
Fragments arranged by size:
4545 2948 593
XmnI GAAnn'nnTTC
Cuts at: 3085 6215 3085
Size: 3130 4956
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5894 276
Size: 53 83 186 936 4360 2468
Fragments arranged by size:
4360 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I
EcoNI FseI FspAI KasI NarI NruI NsiI PacI
PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI