Restriction map for
pFN21AA1714
(Circular) (Six-base) MAPSORT of: pFN21AA1714.seq Check: 9568 from: 1 to: 8086
pFN21AA1714 8086 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3326 4287 3326
Size: 961 7125
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5896 278
Size: 53 83 186 936 4360 2468
Fragments arranged by size:
4360 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5429 1937
Size: 3492 4594
Acc65I G'GTAC_C
Cuts at: 5407 5407
Size: 8086
AclI AA'CG_TT
Cuts at: 1563 5385 6213 6586 1563
Size: 3822 828 373 3063
Fragments arranged by size:
3822 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1995 2119 6149 7533 7801 1632
Size: 363 124 4030 1384 268 1917
Fragments arranged by size:
4030 1917 1384 363 268 124
AfeI AGC'GCT
Cuts at: 4028 4028
Size: 8086
AflII C'TTAA_G
Cuts at: 820 1017 3272 820
Size: 197 2255 5634
Fragments arranged by size:
5634 2255 197
AflIII A'CryG_T
Cuts at: 1203 4452 6985 7962 1203
Size: 3249 2533 977 1327
Fragments arranged by size:
3249 2533 1327 977
AgeI A'CCGG_T
Cuts at: 6917 6917
Size: 8086
AhdI GACnn_n'nnGTC
Cuts at: 3178 6815 3178
Size: 3637 4449
AleI CACnn'nnGTG
Cuts at: 1166 4550 1166
Size: 3384 4702
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4903 1184
Size: 3719 4367
AlwNI CAG_nnn'CTG
Cuts at: 3341 3436 3669 4042 5829 7401 3341
Size: 95 233 373 1787 1572 4026
Fragments arranged by size:
4026 1787 1572 373 233 95
ApaLI G'TGCA_C
Cuts at: 2429 6143 7299 2429
Size: 3714 1156 3216
Fragments arranged by size:
3714 3216 1156
ApoI r'AATT_y
Cuts at: 1493 2636 2661 2946 4381 5395 5621 5773 7882
Size: 1143 25 285 1435 1014 226 152 2109
Cuts at: 7882 1493
Size: 1697
Fragments arranged by size:
2109 1697 1435 1143 1014 285 226 152
25
AseI AT'TA_AT
Cuts at: 160 1032 2711 6640 160
Size: 872 1679 3929 1606
Fragments arranged by size:
3929 1679 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8086
AvaI C'yCGr_G
Cuts at: 1870 1943 2170 5411 1870
Size: 73 227 3241 4545
Fragments arranged by size:
4545 3241 227 73
AvrII C'CTAG_G
Cuts at: 2123 2495 2123
Size: 372 7714
BamHI G'GATC_C
Cuts at: 3577 5416 5783 3577
Size: 1839 367 5880
Fragments arranged by size:
5880 1839 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3938 3951 4068 4632 5407
Size: 325 217 627 2151 13 117 564 775
Cuts at: 5407 6862 618
Size: 1455 1842
Fragments arranged by size:
2151 1842 1455 775 627 564 325 217
117 13
BanII G_rGCy'C
Cuts at: 1391 2069 2312 2841 3134 5183 5405 1391
Size: 678 243 529 293 2049 222 4072
Fragments arranged by size:
4072 2049 678 529 293 243 222
BbeI G_GCGC'C
Cuts at: 4072 4072
Size: 8086
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5149 5183 6277 6311 1903
Size: 34 3212 34 1094 34 3678
Fragments arranged by size:
3678 3212 1094 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5978 7188 5978
Size: 1210 6876
BclI T'GATC_A
Cuts at: 1548 4681 5127 1548
Size: 3133 446 4507
Fragments arranged by size:
4507 3133 446
BfrBI ATG'CAT
Cuts at: 4797 5273 4797
Size: 476 7610
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6697 136
Size: 107 122 71 631 605 5025 1525
Fragments arranged by size:
5025 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8081 8081
Size: 8086
BlpI GC'TnA_GC
Cuts at: 2754 5503 2754
Size: 2749 5337
Bme1580I G_kGCm'C
Cuts at: 1790 2433 3943 3954 4725 6147 7303 1790
Size: 643 1510 11 771 1422 1156 2573
Fragments arranged by size:
2573 1510 1422 1156 771 643 11
BmgBI CAC'GTC
Cuts at: 1318 3245 4453 1318
Size: 1927 1208 4951
Fragments arranged by size:
4951 1927 1208
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2326 3688 4147
Size: 416 53 83 399 24 909 1362 459
Cuts at: 4147 4352 5817 6775 442
Size: 205 1465 958 1753
Fragments arranged by size:
1753 1465 1362 958 909 459 416 399
205 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8086
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2426 3118 3608 5059 6746 1239
Size: 1187 692 490 1451 1687 2579
Fragments arranged by size:
2579 1687 1451 1187 692 490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 6211 7076 7374 2015
Size: 4196 865 298 2727
Fragments arranged by size:
4196 2727 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3740 4809 6749 882
Size: 617 2241 1069 1940 2219
Fragments arranged by size:
2241 2219 1940 1069 617
BsaAI yAC'GTr
Cuts at: 493 1204 4639 5877 493
Size: 711 3435 1238 2702
Fragments arranged by size:
3435 2702 1238 711
BsaBI GATnn'nnATC
Cuts at: 2516 5256 8080 2516
Size: 2740 2824 2522
Fragments arranged by size:
2824 2740 2522
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3885 4069 4856 5893
Size: 53 83 186 936 2352 184 787 1037
Cuts at: 5893 6275 6910 275
Size: 382 635 1451
Fragments arranged by size:
2352 1451 1037 936 787 635 382 186
184 83 53
BsaWI w'CCGG_w
Cuts at: 6519 6917 7191 7338 7749 6519
Size: 398 274 147 411 6856
Fragments arranged by size:
6856 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2758 2788 3015 3045 3695
Size: 30 165 30 1355 30 227 30 650
Cuts at: 3695 3725 4859 4889 5140 5170 1178
Size: 30 1134 30 251 30 4094
Fragments arranged by size:
4094 1355 1134 650 251 227 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2134 2831 5017 1186
Size: 948 697 2186 4255
Fragments arranged by size:
4255 2186 948 697
BseYI C'CCAG_C
Cuts at: 3604 3662 3944 7289 7785 3604
Size: 58 282 3345 496 3905
Fragments arranged by size:
3905 3345 496 282 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3923 4619 4899 1163
Size: 573 2187 696 280 4350
Fragments arranged by size:
4350 2187 696 573 280
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5536 6297 7325 1540
Size: 275 186 3535 761 1028 2301
Fragments arranged by size:
3535 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2312 2433 2578 2841 3134 3450 4278 5183 6147
Size: 121 145 263 293 316 828 905 964
Cuts at: 6147 6232 7303 2312
Size: 85 1071 3095
Fragments arranged by size:
3095 1071 964 905 828 316 293 263
145 121 85
BsiWI C'GTAC_G
Cuts at: 3978 3978
Size: 8086
BsmI GAATG_Cn'
Cuts at: 2821 2972 4797 5597 5690 2821
Size: 151 1825 800 93 5217
Fragments arranged by size:
5217 1825 800 151 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2613 4674 2613
Size: 2061 6025
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2312 2433 2578 2841 3134 3450
Size: 399 279 243 121 145 263 293 316
Cuts at: 3450 3943 3954 4278 4725 5183 5405 6147 6232
Size: 493 11 324 447 458 222 742 85
Cuts at: 6232 7303 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 493 458 447 399 324
316 293 279 263 243 222 145 121
85 11
BspEI T'CCGG_A
Cuts at: 7749 7749
Size: 8086
BspHI T'CATG_A
Cuts at: 3069 5975 7705 3069
Size: 2906 1730 3450
Fragments arranged by size:
3450 2906 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2842 3326 3758 4287 5441 844
Size: 1998 484 432 529 1154 3489
Fragments arranged by size:
3489 1998 1154 529 484 432
BsrBI CCG'CTC
Cuts at: 1394 1823 5973 1394
Size: 429 4150 3507
Fragments arranged by size:
4150 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2662 2755 4921 6581 6755 65
Size: 1183 1414 93 2166 1660 174 1396
Fragments arranged by size:
2166 1660 1414 1396 1183 174 93
BsrFI r'CCGG_y
Cuts at: 1700 1810 6730 6917 1700
Size: 110 4920 187 2869
Fragments arranged by size:
4920 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8086
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8086
BssSI C'ACGA_G
Cuts at: 4080 6146 7158 4080
Size: 2066 1012 5008
Fragments arranged by size:
5008 2066 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5015 794
Size: 4221 3865
BstBI TT'CG_AA
Cuts at: 3199 6890 3199
Size: 3691 4395
BstEII G'GTnAC_C
Cuts at: 1184 2320 3925 4948 1184
Size: 1136 1605 1023 4322
Fragments arranged by size:
4322 1605 1136 1023
BstXI CCAn_nnnn'nTGG
Cuts at: 2914 4101 2914
Size: 1187 6899
BstYI r'GATC_y
Cuts at: 1972 2917 3063 3577 4831 5416 5783 6168 6185
Size: 945 146 514 1254 585 367 385 17
Cuts at: 6185 7637 7723 7735 8081 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1254 945 585 514 385 367
346 146 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3545 3545
Size: 8086
BtgI C'CryG_G
Cuts at: 513 1065 2004 2164 2865 4008 4360 4432 513
Size: 552 939 160 701 1143 352 72 4167
Fragments arranged by size:
4167 1143 939 701 552 352 160 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7347
Fragments arranged by size:
7347 683 56
BtsI GCAGTG_nn'
Cuts at: 2277 3365 4349 4443 4885 5602 6396 6416 2277
Size: 1088 984 94 442 717 794 20 3947
Fragments arranged by size:
3947 1088 984 794 717 442 94 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8086
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8051
DraI TTT'AAA
Cuts at: 2607 2641 5290 5390 2607
Size: 34 2649 100 5303
Fragments arranged by size:
5303 2649 100 34
DraIII CAC_nnn'GTG
Cuts at: 3370 4639 5320 3370
Size: 1269 681 6136
Fragments arranged by size:
6136 1269 681
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2852 4256 4908 7093 809
Size: 507 1536 1404 652 2185 1802
Fragments arranged by size:
2185 1802 1536 1404 652 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4228 4767 5533
Size: 54 1425 50 275 18 2398 539 766
Cuts at: 5533 6422 8051 8
Size: 889 1629 43
Fragments arranged by size:
2398 1629 1425 889 766 539 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5533 1537
Size: 275 3721 4090
Fragments arranged by size:
4090 3721 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2188 3305 4677 4757 6016 1353
Size: 835 1117 1372 80 1259 3423
Fragments arranged by size:
3423 1372 1259 1117 835 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4037 6673 7047 7193 387
Size: 1064 138 2448 2636 374 146 1280
Fragments arranged by size:
2636 2448 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2310 2839 3132 5181 2310
Size: 529 293 2049 5215
Fragments arranged by size:
5215 2049 529 293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1995 2119 2426 3118 3608 5059 6149
Size: 393 363 124 307 692 490 1451 1090
Cuts at: 6149 6746 7533 7801 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1451 1090 787 692 597 490 393
363 307 268 124
EcoNI CCTnn'n_nnAGG
Cuts at: 2122 2122
Size: 8086
EcoO109I rG'GnC_Cy
Cuts at: 3619 3619
Size: 8086
EcoRI G'AATT_C
Cuts at: 2661 2946 4381 5395 5773 2661
Size: 285 1435 1014 378 4974
Fragments arranged by size:
4974 1435 1014 378 285
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2741 2746 2741
Size: 5 8081
FspI TGC'GCA
Cuts at: 6592 6592
Size: 8086
HaeII r_GCGC'y
Cuts at: 1678 3605 4030 4072 7233 1678
Size: 1927 425 42 3161 2531
Fragments arranged by size:
3161 2531 1927 425 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3164 3196 3482 3514 3695
Size: 32 135 32 1592 32 286 32 181
Cuts at: 3695 3727 5112 5144 6727 6759 6801 6833 1373
Size: 32 1385 32 1583 32 42 32 2626
Fragments arranged by size:
2626 1592 1583 1385 286 181 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2857 5430 5676 669
Size: 988 281 919 2573 246 3079
Fragments arranged by size:
3079 2573 988 919 281 246
HindIII A'AGCT_T
Cuts at: 748 2723 2986 748
Size: 1975 263 5848
Fragments arranged by size:
5848 1975 263
HpaI GTT'AAC
Cuts at: 5676 5676
Size: 8086
KasI G'GCGC_C
Cuts at: 4068 4068
Size: 8086
KpnI G_GTAC'C
Cuts at: 5411 5411
Size: 8086
MfeI C'AATT_G
Cuts at: 5685 5685
Size: 8086
MluI A'CGCG_T
Cuts at: 7962 7962
Size: 8086
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2975 4090 7200 7384 1305
Size: 1670 1115 3110 184 2007
Fragments arranged by size:
3110 2007 1670 1115 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6264
Fragments arranged by size:
6264 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3399 4550 5103 6044 6403 6562 518
Size: 648 2233 1151 553 941 359 159 2042
Fragments arranged by size:
2233 2042 1151 941 648 553 359 159
MspA1I CmG'CkG
Cuts at: 1606 3425 3948 3986 4000 4010 4039 4362 5502
Size: 1819 523 38 14 10 29 323 1140
Cuts at: 5502 6179 7327 7572 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1819 1148 1140 677 523 323 245
38 29 14 10
NarI GG'CG_CC
Cuts at: 4069 4069
Size: 8086
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6595
Fragments arranged by size:
6595 939 552
NdeI CA'TA_TG
Cuts at: 387 3396 387
Size: 3009 5077
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8086
NotI GC'GGCC_GC
Cuts at: 5533 5533
Size: 8086
NsiI A_TGCA'T
Cuts at: 4799 5275 4799
Size: 476 7610
NspI r_CATG'y
Cuts at: 1132 1229 4541 5444 6989 1132
Size: 97 3312 903 1545 2229
Fragments arranged by size:
3312 2229 1545 903 97
PacI TTA_AT'TAA
Cuts at: 2715 2715
Size: 8086
PasI CC'CwG_GG
Cuts at: 1348 2791 3523 3847 1348
Size: 1443 732 324 5587
Fragments arranged by size:
5587 1443 732 324
PciI A'CATG_T
Cuts at: 6985 6985
Size: 8086
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8086
PfoI T'CCnGG_A
Cuts at: 2401 3134 2401
Size: 733 7353
PmeI GTTT'AAAC
Cuts at: 5390 5390
Size: 8086
PmlI CAC'GTG
Cuts at: 4639 4639
Size: 8086
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6155 6187 7668 7700 6155
Size: 32 1481 32 6541
Fragments arranged by size:
6541 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3619 3619
Size: 8086
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8086
PsiI TTA'TAA
Cuts at: 3018 5656 3018
Size: 2638 5448
PstI C_TGCA'G
Cuts at: 830 4812 4892 5438 830
Size: 3982 80 546 3478
Fragments arranged by size:
3982 3478 546 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8086
PvuII CAG'CTG
Cuts at: 3425 4000 3425
Size: 575 7511
RsrII CG'GwC_CG
Cuts at: 4005 4005
Size: 8086
SacI G_AGCT'C
Cuts at: 2312 2841 3134 5183 2312
Size: 529 293 2049 5215
Fragments arranged by size:
5215 2049 529 293
SacII CC_GC'GG
Cuts at: 4011 4363 4011
Size: 352 7734
SalI G'TCGA_C
Cuts at: 1936 5428 1936
Size: 3492 4594
SapI GCTCTTCn'nnn_
Cuts at: 4757 4757
Size: 8086
SbfI CC_TGCA'GG
Cuts at: 5438 5438
Size: 8086
ScaI AGT'ACT
Cuts at: 6334 8046 6334
Size: 1712 6374
SfcI C'TryA_G
Cuts at: 826 1045 2434 4808 4888 5434 6569 7250 7441
Size: 219 1389 2374 80 546 1135 681 191
Cuts at: 7441 826
Size: 1471
Fragments arranged by size:
2374 1471 1389 1135 681 546 219 191
80
SfoI GGC'GCC
Cuts at: 4070 4070
Size: 8086
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8086
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8086
SmaI CCC'GGG
Cuts at: 1872 2172 5413 1872
Size: 300 3241 4545
Fragments arranged by size:
4545 3241 300
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2030 3272 6190 7091 7353 820
Size: 197 926 87 1242 2918 901 262 1553
Fragments arranged by size:
2918 1553 1242 926 901 262 197 87
SnaBI TAC'GTA
Cuts at: 493 5877 493
Size: 5384 2702
SpeI A'CTAG_T
Cuts at: 152 4244 152
Size: 4092 3994
SphI G_CATG'C
Cuts at: 1132 4541 5444 1132
Size: 3409 903 3774
Fragments arranged by size:
3774 3409 903
SspI AAT'ATT
Cuts at: 5 52 6010 5
Size: 47 5958 2081
Fragments arranged by size:
5958 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8086
StyI C'CwwG_G
Cuts at: 513 1065 2004 2123 2257 2293 2495 3320 5525
Size: 552 939 119 134 36 202 825 2205
Cuts at: 5525 513
Size: 3074
Fragments arranged by size:
3074 2205 939 825 552 202 134 119
36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2306 2308 2985 4621 4791 6113 6130
Size: 1255 726 2 677 1636 170 1322 17
Cuts at: 6130 6283 6468 6913 325
Size: 153 185 445 1498
Fragments arranged by size:
1636 1498 1322 1255 726 677 445 185
170 153 17 2
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3589 3864 4169
Size: 274 80 33 51 1443 1612 275 305
Cuts at: 4169 6332 8044 96
Size: 2163 1712 138
Fragments arranged by size:
2163 1712 1612 1443 305 275 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2758 2790 2807 2839 3096 3128 3356 3388 4859
Size: 32 17 32 257 32 228 32 1471
Cuts at: 4859 4891 5124 5140 5156 5172 8046 8078 2758
Size: 32 233 16 16 16 2874 32 2766
Fragments arranged by size:
2874 2766 1471 257 233 228 32 32
32 32 32 32 17 16 16 16
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8086
XbaI T'CTAG_A
Cuts at: 5422 5422
Size: 8086
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2300 3142 4101 4754 2011
Size: 289 842 959 653 5343
Fragments arranged by size:
5343 959 842 653 289
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8086
XmaI C'CCGG_G
Cuts at: 1870 2170 5411 1870
Size: 300 3241 4545
Fragments arranged by size:
4545 3241 300
XmnI GAAnn'nnTTC
Cuts at: 6215 6215
Size: 8086
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5894 276
Size: 53 83 186 936 4360 2468
Fragments arranged by size:
4360 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BbvCI BplI Bpu10I BstZ17I
EcoRV FseI FspAI NaeI NgoMIV NruI PspOMI PspXI
PsrI SanDI SexAI SfiI SrfI SwaI