Restriction map for
pFN21AA1708
(Circular) (Six-base) MAPSORT of: pFN21AA1708.seq Check: 2859 from: 1 to: 9688
pFN21AA1708 9688 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3398 4546 3398
Size: 1148 8540
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7498 278
Size: 53 83 186 936 5962 2468
Fragments arranged by size:
5962 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5167 6416 7031 1937
Size: 3230 1249 615 4594
Fragments arranged by size:
4594 3230 1249 615
Acc65I G'GTAC_C
Cuts at: 7009 7009
Size: 9688
AclI AA'CG_TT
Cuts at: 1563 3077 7815 8188 1563
Size: 1514 4738 373 3063
Fragments arranged by size:
4738 3063 1514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2867 3039 3176 4467 5108 5361 5466 5745
Size: 1235 172 137 1291 641 253 105 279
Cuts at: 5745 5960 6015 6231 6677 7751 9135 9403 1632
Size: 215 55 216 446 1074 1384 268 1917
Fragments arranged by size:
1917 1384 1291 1235 1074 641 446 279
268 253 216 215 172 137 105 55
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9491
AflIII A'CryG_T
Cuts at: 1203 2579 2962 3264 8587 9564 1203
Size: 1376 383 302 5323 977 1327
Fragments arranged by size:
5323 1376 1327 977 383 302
AgeI A'CCGG_T
Cuts at: 8519 8519
Size: 9688
AhdI GACnn_n'nnGTC
Cuts at: 6561 8417 6561
Size: 1856 7832
AleI CACnn'nnGTG
Cuts at: 1166 5977 1166
Size: 4811 4877
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3180 1184
Size: 1996 7692
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4507 4539 4507
Size: 32 9656
AlwNI CAG_nnn'CTG
Cuts at: 2837 3707 3869 3985 4568 5753 6771 7431 9003
Size: 870 162 116 583 1185 1018 660 1572
Cuts at: 9003 2837
Size: 3522
Fragments arranged by size:
3522 1572 1185 1018 870 660 583 162
116
ApaLI G'TGCA_C
Cuts at: 7745 8901 7745
Size: 1156 8532
ApoI r'AATT_y
Cuts at: 1493 2532 2556 2789 4029 4614 5667 6912 6997
Size: 1039 24 233 1240 585 1053 1245 85
Cuts at: 6997 7223 7375 9484 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1245 1240 1053 1039 585 233
226 152 85 24
AseI AT'TA_AT
Cuts at: 160 1032 5052 8242 160
Size: 872 4020 3190 1606
Fragments arranged by size:
4020 3190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9688
AvaI C'yCGr_G
Cuts at: 1870 1943 2349 3011 7013 1870
Size: 73 406 662 4002 4545
Fragments arranged by size:
4545 4002 662 406 73
AvrII C'CTAG_G
Cuts at: 2131 6439 2131
Size: 4308 5380
BamHI G'GATC_C
Cuts at: 7018 7385 7018
Size: 367 9321
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2012 6353 6742 7009 8464
Size: 325 217 627 225 4341 389 267 1455
Cuts at: 8464 618
Size: 1842
Fragments arranged by size:
4341 1842 1455 627 389 325 267 225
217
BanII G_rGCy'C
Cuts at: 1391 2030 2457 2675 3204 4600 5598 6550 7007
Size: 639 427 218 529 1396 998 952 457
Cuts at: 7007 1391
Size: 4072
Fragments arranged by size:
4072 1396 998 952 639 529 457 427
218
BbeI G_GCGC'C
Cuts at: 2016 2016
Size: 9688
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2119 5114 5367 6163 6237 928
Size: 971 220 2995 253 796 74 4379
Fragments arranged by size:
4379 2995 971 796 253 220 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4562 4596 7879 7913 1903
Size: 34 2625 34 3283 34 3678
Fragments arranged by size:
3678 3283 2625 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2234 4030 6799 7580 8790 2234
Size: 1796 2769 781 1210 3132
Fragments arranged by size:
3132 2769 1796 1210 781
BclI T'GATC_A
Cuts at: 1548 3379 3790 1548
Size: 1831 411 7446
Fragments arranged by size:
7446 1831 411
BfrBI ATG'CAT
Cuts at: 3347 4583 3347
Size: 1236 8452
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8299 136
Size: 107 122 71 631 605 6627 1525
Fragments arranged by size:
6627 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2193 3466 5284 5380 6487 6945 6961 9683 2193
Size: 1273 1818 96 1107 458 16 2722 2198
Fragments arranged by size:
2722 2198 1818 1273 1107 458 96 16
BlpI GC'TnA_GC
Cuts at: 7105 7105
Size: 9688
Bme1580I G_kGCm'C
Cuts at: 1790 7749 8905 1790
Size: 5959 1156 2573
Fragments arranged by size:
5959 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9688
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2345 6102 6122
Size: 416 53 83 399 24 928 3757 20
Cuts at: 6122 6749 7419 8377 442
Size: 627 670 958 1753
Fragments arranged by size:
3757 1753 958 928 670 627 416 399
83 53 24 20
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2295 2406 2571 2877 5902 6333 8348 1239
Size: 1056 111 165 306 3025 431 2015 2579
Fragments arranged by size:
3025 2579 2015 1056 431 306 165 111
Bpu10I CC'TnA_GC
Cuts at: 4542 5250 4542
Size: 708 8980
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4763 4802 7813 8678 8976 4763
Size: 39 3011 865 298 5475
Fragments arranged by size:
5475 3011 865 298 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4047 8351 882
Size: 617 2548 4304 2219
Fragments arranged by size:
4304 2548 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2582 7479 493
Size: 711 1378 4897 2702
Fragments arranged by size:
4897 2702 1378 711
BsaBI GATnn'nnATC
Cuts at: 9682 9682
Size: 9688
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2013 7495 7877 8512
Size: 53 83 186 936 480 5482 382 635
Cuts at: 8512 275
Size: 1451
Fragments arranged by size:
5482 1451 936 635 480 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 8121 8519 8793 8940 9351 8121
Size: 398 274 147 411 8458
Fragments arranged by size:
8458 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2012 2042 2162 2192 3559
Size: 30 165 30 609 30 120 30 1367
Cuts at: 3559 3589 5900 5930 1178
Size: 30 2311 30 4936
Fragments arranged by size:
4936 2311 1367 609 165 120 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3437 3824 3827 3830 3833 3836 4805 5881
Size: 2251 387 3 3 3 3 969 1076
Cuts at: 5881 6032 1186
Size: 151 4842
Fragments arranged by size:
4842 2251 1076 969 387 151 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2181 4499 4599 8891 9387 2181
Size: 2318 100 4292 496 2482
Fragments arranged by size:
4292 2482 2318 496 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4581 5044 1163
Size: 573 2845 463 5807
Fragments arranged by size:
5807 2845 573 463
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7138 7899 8927 1540
Size: 275 186 5137 761 1028 2301
Fragments arranged by size:
5137 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2030 2457 2675 3204 5598 6018 6550 7749 7834
Size: 427 218 529 2394 420 532 1199 85
Cuts at: 7834 8905 2030
Size: 1071 2813
Fragments arranged by size:
2813 2394 1199 1071 532 529 427 420
218 85
BsmI GAATG_Cn'
Cuts at: 2443 3283 6228 7199 7292 2443
Size: 840 2945 971 93 4839
Fragments arranged by size:
4839 2945 971 840 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3329 3329
Size: 9688
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2457 2675 3204 4600 5598 6018
Size: 399 240 427 218 529 1396 998 420
Cuts at: 6018 6550 7007 7749 7834 8905 1391
Size: 532 457 742 85 1071 2174
Fragments arranged by size:
2174 1396 1071 998 742 532 529 457
427 420 399 240 218 85
BspEI T'CCGG_A
Cuts at: 9351 9351
Size: 9688
BspHI T'CATG_A
Cuts at: 2536 3033 3271 3793 5385 6838 7577 9307 2536
Size: 497 238 522 1592 1453 739 1730 2917
Fragments arranged by size:
2917 1730 1592 1453 739 522 497 238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3315 3398 4546 5091 7043 844
Size: 2471 83 1148 545 1952 3489
Fragments arranged by size:
3489 2471 1952 1148 545 83
BsrBI CCG'CTC
Cuts at: 1394 1823 7575 1394
Size: 429 5752 3507
Fragments arranged by size:
5752 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2926 8183 8357 65
Size: 1183 1678 5257 174 1396
Fragments arranged by size:
5257 1678 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4648 5799 8332 8519 1700
Size: 110 2838 1151 2533 187 2869
Fragments arranged by size:
2869 2838 2533 1151 187 110
BsrGI T'GTAC_A
Cuts at: 96 2096 96
Size: 2000 7688
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9688
BssSI C'ACGA_G
Cuts at: 2493 7748 8760 2493
Size: 5255 1012 3421
Fragments arranged by size:
5255 3421 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9688
BstBI TT'CG_AA
Cuts at: 3514 6232 8492 3514
Size: 2718 2260 4710
Fragments arranged by size:
4710 2718 2260
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9688
BstXI CCAn_nnnn'nTGG
Cuts at: 5446 5446
Size: 9688
BstYI r'GATC_y
Cuts at: 1972 2193 2834 3466 5284 5380 5912 6397 6487
Size: 221 641 632 1818 96 532 485 90
Cuts at: 6487 6945 6961 7018 7385 7770 7787 9239 9325
Size: 458 16 57 367 385 17 1452 86
Cuts at: 9325 9337 9683 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1818 1452 641 632 532 485 458
385 367 346 221 96 90 86 57
17 16 12
Bsu36I CC'TnA_GG
Cuts at: 2121 2121
Size: 9688
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 9136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8949
Fragments arranged by size:
8949 683 56
BtsI GCAGTG_nn'
Cuts at: 2618 3504 5476 7204 7998 8018 2618
Size: 886 1972 1728 794 20 4288
Fragments arranged by size:
4288 1972 1728 886 794 20
ClaI AT'CG_AT
Cuts at: 1554 4953 1554
Size: 3399 6289
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9653
DraI TTT'AAA
Cuts at: 2431 5343 6992 2431
Size: 2912 1649 5127
Fragments arranged by size:
5127 2912 1649
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8695 809
Size: 507 7379 1802
Fragments arranged by size:
7379 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4696 7135 8024
Size: 54 1425 50 275 18 2866 2439 889
Cuts at: 8024 9653 8
Size: 1629 43
Fragments arranged by size:
2866 2439 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7135 1537
Size: 275 5323 4090
Fragments arranged by size:
5323 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2460 3234 3801 3996 4443 4467 4920 6050
Size: 1107 774 567 195 447 24 453 1130
Cuts at: 6050 7618 1353
Size: 1568 3423
Fragments arranged by size:
3423 1568 1130 1107 774 567 453 447
195 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8275 8649 8795 387
Size: 1064 138 6686 374 146 1280
Fragments arranged by size:
6686 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2028 2455 2673 3202 5596 6548 2028
Size: 427 218 529 2394 952 5168
Fragments arranged by size:
5168 2394 952 529 427 218
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2295 2406 2571 2867 2877 3039 3176
Size: 393 663 111 165 296 10 162 137
Cuts at: 3176 4467 5108 5361 5466 5745 5902 5960 6015
Size: 1291 641 253 105 279 157 58 55
Cuts at: 6015 6231 6333 6677 7751 8348 9135 9403 1239
Size: 216 102 344 1074 597 787 268 1524
Fragments arranged by size:
1524 1291 1074 787 663 641 597 393
344 296 279 268 253 216 165 162
157 137 111 105 102 58 55 10
EcoNI CCTnn'n_nnAGG
Cuts at: 5920 5920
Size: 9688
EcoO109I rG'GnC_Cy
Cuts at: 2470 6443 2470
Size: 3973 5715
EcoRI G'AATT_C
Cuts at: 2532 4614 6997 7375 2532
Size: 2082 2383 378 4845
Fragments arranged by size:
4845 2383 2082 378
EcoRV GAT'ATC
Cuts at: 6208 6208
Size: 9688
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2489 5592 6278 6566 6707 2489
Size: 3103 686 288 141 5470
Fragments arranged by size:
5470 3103 686 288 141
FspI TGC'GCA
Cuts at: 8194 8194
Size: 9688
HaeII r_GCGC'y
Cuts at: 1678 2016 3548 8835 1678
Size: 338 1532 5287 2531
Fragments arranged by size:
5287 2531 1532 338
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2012 2044 2162 2194 3121
Size: 32 135 32 440 32 118 32 927
Cuts at: 3121 3153 3857 3889 4919 4951 6382 6414 6547
Size: 32 704 32 1030 32 1431 32 133
Cuts at: 6547 6579 6820 6852 8329 8361 8403 8435 1373
Size: 32 241 32 1477 32 42 32 2626
Fragments arranged by size:
2626 1477 1431 1030 927 704 440 241
135 133 118 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2317 5168 6429 7032 7278 669
Size: 988 281 379 2851 1261 603 246 3079
Fragments arranged by size:
3079 2851 1261 988 603 379 281 246
HindIII A'AGCT_T
Cuts at: 748 5390 748
Size: 4642 5046
HpaI GTT'AAC
Cuts at: 2317 6429 7278 2317
Size: 4112 849 4727
Fragments arranged by size:
4727 4112 849
KasI G'GCGC_C
Cuts at: 2012 2012
Size: 9688
KpnI G_GTAC'C
Cuts at: 7013 7013
Size: 9688
MfeI C'AATT_G
Cuts at: 4229 7287 4229
Size: 3058 6630
MluI A'CGCG_T
Cuts at: 9564 9564
Size: 9688
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4812 8802 8986 1305
Size: 3507 3990 184 2007
Fragments arranged by size:
3990 3507 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7866
Fragments arranged by size:
7866 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2702 3276 3798 4766 5444 5977 6524
Size: 648 1536 574 522 968 678 533 547
Cuts at: 6524 6594 7646 8005 8164 518
Size: 70 1052 359 159 2042
Fragments arranged by size:
2042 1536 1052 968 678 648 574 547
533 522 359 159 70
MspA1I CmG'CkG
Cuts at: 1606 3558 4565 6893 7104 7781 8929 9174 1606
Size: 1952 1007 2328 211 677 1148 245 2120
Fragments arranged by size:
2328 2120 1952 1148 1007 677 245 211
NaeI GCC'GGC
Cuts at: 4650 5801 4650
Size: 1151 8537
NarI GG'CG_CC
Cuts at: 2013 2013
Size: 9688
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 9136
NdeI CA'TA_TG
Cuts at: 387 5220 5704 6860 387
Size: 4833 484 1156 3215
Fragments arranged by size:
4833 3215 1156 484
NgoMIV G'CCGG_C
Cuts at: 4648 5799 4648
Size: 1151 8537
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9688
NotI GC'GGCC_GC
Cuts at: 7135 7135
Size: 9688
NsiI A_TGCA'T
Cuts at: 3349 4585 3349
Size: 1236 8452
NspI r_CATG'y
Cuts at: 1132 1229 2687 2966 3045 3268 4193 7046 8591
Size: 97 1458 279 79 223 925 2853 1545
Cuts at: 8591 1132
Size: 2229
Fragments arranged by size:
2853 2229 1545 1458 925 279 223 97
79
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9688
PciI A'CATG_T
Cuts at: 2962 3264 8587 2962
Size: 302 5323 4063
Fragments arranged by size:
5323 4063 302
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4426 1108
Size: 3318 6370
PfoI T'CCnGG_A
Cuts at: 2636 2636
Size: 9688
PmeI GTTT'AAAC
Cuts at: 6992 6992
Size: 9688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2662 2694 5425 5457 7757 7789 9270 9302 2662
Size: 32 2731 32 2300 32 1481 32 3048
Fragments arranged by size:
3048 2731 2300 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2470 2470
Size: 9688
PshAI GACnn'nnGTC
Cuts at: 1613 4483 1613
Size: 2870 6818
PsiI TTA'TAA
Cuts at: 7258 7258
Size: 9688
PstI C_TGCA'G
Cuts at: 830 3981 4164 5047 6776 6892 7040 830
Size: 3151 183 883 1729 116 148 3478
Fragments arranged by size:
3478 3151 1729 883 183 148 116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9688
PvuII CAG'CTG
Cuts at: 4565 6893 4565
Size: 2328 7360
SacI G_AGCT'C
Cuts at: 2030 2457 2675 3204 5598 6550 2030
Size: 427 218 529 2394 952 5168
Fragments arranged by size:
5168 2394 952 529 427 218
SalI G'TCGA_C
Cuts at: 1936 5166 7030 1936
Size: 3230 1864 4594
Fragments arranged by size:
4594 3230 1864
SapI GCTCTTCn'nnn_
Cuts at: 4467 4467
Size: 9688
SbfI CC_TGCA'GG
Cuts at: 7040 7040
Size: 9688
ScaI AGT'ACT
Cuts at: 5157 5323 7936 9648 5157
Size: 166 2613 1712 5197
Fragments arranged by size:
5197 2613 1712 166
SexAI A'CCwGG_T
Cuts at: 4056 4056
Size: 9688
SfcI C'TryA_G
Cuts at: 826 1045 2250 2862 2971 2999 3287 3977 4160
Size: 219 1205 612 109 28 288 690 183
Cuts at: 4160 5043 6417 6772 6888 7036 8171 8852 9043
Size: 883 1374 355 116 148 1135 681 191
Cuts at: 9043 826
Size: 1471
Fragments arranged by size:
1471 1374 1205 1135 883 690 681 612
355 288 219 191 183 148 116 109
28
SfoI GGC'GCC
Cuts at: 2014 2014
Size: 9688
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9688
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9688
SmaI CCC'GGG
Cuts at: 1872 7015 1872
Size: 5143 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2349 4742 4817 7792 8693 8955
Size: 197 926 406 2393 75 2975 901 262
Cuts at: 8955 820
Size: 1553
Fragments arranged by size:
2975 2393 1553 926 901 406 262 197
75
SnaBI TAC'GTA
Cuts at: 493 7479 493
Size: 6986 2702
SpeI A'CTAG_T
Cuts at: 152 5489 152
Size: 5337 4351
SphI G_CATG'C
Cuts at: 1132 7046 1132
Size: 5914 3774
SspI AAT'ATT
Cuts at: 5 52 7612 5
Size: 47 7560 2081
Fragments arranged by size:
7560 2081 47
StuI AGG'CCT
Cuts at: 1517 4637 1517
Size: 3120 6568
StyI C'CwwG_G
Cuts at: 513 1065 2131 2934 5730 6439 6507 6732 7127
Size: 552 1066 803 2796 709 68 225 395
Cuts at: 7127 513
Size: 3074
Fragments arranged by size:
3074 2796 1066 803 709 552 395 225
68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2615 6584 6768 6890 7715 7732 7885
Size: 1255 1035 3969 184 122 825 17 153
Cuts at: 7885 8070 8515 325
Size: 185 445 1498
Fragments arranged by size:
3969 1498 1255 1035 825 445 185 184
153 122 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2096 3210 5155
Size: 274 80 33 51 1443 119 1114 1945
Cuts at: 5155 5321 5492 7934 9646 96
Size: 166 171 2442 1712 138
Fragments arranged by size:
2442 1945 1712 1443 1114 274 171 166
138 119 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5900 5932 9648 9680 5900
Size: 32 3716 32 5908
Fragments arranged by size:
5908 3716 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4327 1316
Size: 3011 6677
XbaI T'CTAG_A
Cuts at: 4331 5287 6644 7024 4331
Size: 956 1357 380 6995
Fragments arranged by size:
6995 1357 956 380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4789 5443 4789
Size: 654 9034
XhoI C'TCGA_G
Cuts at: 1943 2349 1943
Size: 406 9282
XmaI C'CCGG_G
Cuts at: 1870 7013 1870
Size: 5143 4545
XmnI GAAnn'nnTTC
Cuts at: 4428 7817 4428
Size: 3389 6299
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7496 276
Size: 53 83 186 936 5962 2468
Fragments arranged by size:
5962 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbvCI BplI BsiWI BstZ17I
DraIII FseI FspAI NruI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SfiI SrfI SwaI