Restriction map for
pFN21AA1707
(Circular) (Six-base) MAPSORT of: pFN21AA1707.seq Check: 7017 from: 1 to: 7756
pFN21AA1707 7756 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5566 278
Size: 53 83 186 936 4030 2468
Fragments arranged by size:
4030 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5099 1937
Size: 3162 4594
Acc65I G'GTAC_C
Cuts at: 5077 5077
Size: 7756
AclI AA'CG_TT
Cuts at: 1563 5883 6256 1563
Size: 4320 373 3063
Fragments arranged by size:
4320 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2718 2985 3669 4002 5819 7203 7471 1632
Size: 1086 267 684 333 1817 1384 268 1917
Fragments arranged by size:
1917 1817 1384 1086 684 333 268 267
AflII C'TTAA_G
Cuts at: 820 1017 3278 4259 820
Size: 197 2261 981 4317
Fragments arranged by size:
4317 2261 981 197
AflIII A'CryG_T
Cuts at: 1203 2339 3365 6655 7632 1203
Size: 1136 1026 3290 977 1327
Fragments arranged by size:
3290 1327 1136 1026 977
AgeI A'CCGG_T
Cuts at: 6587 6587
Size: 7756
AhdI GACnn_n'nnGTC
Cuts at: 6485 6485
Size: 7756
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7756
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7756
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3083 3115 3083
Size: 32 7724
AlwNI CAG_nnn'CTG
Cuts at: 5499 7071 5499
Size: 1572 6184
ApaLI G'TGCA_C
Cuts at: 5813 6969 5813
Size: 1156 6600
ApoI r'AATT_y
Cuts at: 1493 2054 2240 3273 3755 4759 5065 5291 5443
Size: 561 186 1033 482 1004 306 226 152
Cuts at: 5443 7552 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1033 1004 561 482 306 226
186 152
AseI AT'TA_AT
Cuts at: 160 1032 6310 160
Size: 872 5278 1606
Fragments arranged by size:
5278 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7756
AvaI C'yCGr_G
Cuts at: 1870 1943 5081 1870
Size: 73 3138 4545
Fragments arranged by size:
4545 3138 73
BamHI G'GATC_C
Cuts at: 2950 4921 5086 5453 2950
Size: 1971 165 367 5253
Fragments arranged by size:
5253 1971 367 165
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5077 6532 618
Size: 325 217 627 3290 1455 1842
Fragments arranged by size:
3290 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2588 5013 5075 1391
Size: 1197 2425 62 4072
Fragments arranged by size:
4072 2425 1197 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2706 2813 4405 928
Size: 971 807 107 1592 4279
Fragments arranged by size:
4279 1592 971 807 107
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5947 5981 1903
Size: 34 4010 34 3678
Fragments arranged by size:
4010 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2382 3586 4564 5648 6858 2382
Size: 1204 978 1084 1210 3280
Fragments arranged by size:
3280 1210 1204 1084 978
BclI T'GATC_A
Cuts at: 1548 2728 1548
Size: 1180 6576
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6367 136
Size: 107 122 71 631 605 4695 1525
Fragments arranged by size:
4695 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4201 7751 4201
Size: 3550 4206
BlpI GC'TnA_GC
Cuts at: 4572 5173 4572
Size: 601 7155
Bme1580I G_kGCm'C
Cuts at: 1790 5817 6973 1790
Size: 4027 1156 2573
Fragments arranged by size:
4027 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7756
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5487 6445 442
Size: 416 53 83 399 24 4070 958 1753
Fragments arranged by size:
4070 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7756
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4226 6416 1239
Size: 2987 2190 2579
Fragments arranged by size:
2987 2579 2190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5881 6746 7044 5881
Size: 865 298 6593
Fragments arranged by size:
6593 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3685 6419 882
Size: 617 2186 2734 2219
Fragments arranged by size:
2734 2219 2186 617
BsaAI yAC'GTr
Cuts at: 493 1204 4616 5547 493
Size: 711 3412 931 2702
Fragments arranged by size:
3412 2702 931 711
BsaBI GATnn'nnATC
Cuts at: 3639 4608 7750 3639
Size: 969 3142 3645
Fragments arranged by size:
3645 3142 969
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5563 5945 6580 275
Size: 53 83 186 936 4030 382 635 1451
Fragments arranged by size:
4030 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6189 6587 6861 7008 7419 6189
Size: 398 274 147 411 6526
Fragments arranged by size:
6526 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4949 4979 1178
Size: 30 165 30 3546 30 3955
Fragments arranged by size:
3955 3546 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7756
BseYI C'CCAG_C
Cuts at: 4557 6959 7455 4557
Size: 2402 496 4858
Fragments arranged by size:
4858 2402 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4662 1163
Size: 573 2926 4257
Fragments arranged by size:
4257 2926 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5206 5967 6995 1540
Size: 275 186 3205 761 1028 2301
Fragments arranged by size:
3205 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5013 5817 5902 6973 5013
Size: 804 85 1071 5796
Fragments arranged by size:
5796 1071 804 85
BsmI GAATG_Cn'
Cuts at: 5267 5360 5267
Size: 93 7663
BsmBI CGTCTCn'nnnn_
Cuts at: 4485 4485
Size: 7756
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2588 5013 5075 5817 5902 6973 1391
Size: 399 798 2425 62 742 85 1071 2174
Fragments arranged by size:
2425 2174 1071 798 742 399 85 62
BspEI T'CCGG_A
Cuts at: 7419 7419
Size: 7756
BspHI T'CATG_A
Cuts at: 5645 7375 5645
Size: 1730 6026
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5111 844
Size: 4267 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5643 1394
Size: 429 3820 3507
Fragments arranged by size:
3820 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2306 3581 6251 6425 65
Size: 1183 1058 1275 2670 174 1396
Fragments arranged by size:
2670 1396 1275 1183 1058 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3065 6400 6587 1700
Size: 110 1255 3335 187 2869
Fragments arranged by size:
3335 2869 1255 187 110
BsrGI T'GTAC_A
Cuts at: 96 3376 4741 96
Size: 3280 1365 3111
Fragments arranged by size:
3280 3111 1365
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7756
BssSI C'ACGA_G
Cuts at: 5816 6828 5816
Size: 1012 6744
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7756
BstBI TT'CG_AA
Cuts at: 6560 6560
Size: 7756
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7756
BstYI r'GATC_y
Cuts at: 1972 2950 3454 3549 4201 4837 4921 5086 5453
Size: 978 504 95 652 636 84 165 367
Cuts at: 5453 5838 5855 7307 7393 7405 7751 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 978 652 636 504 385 367
346 165 95 86 84 17 12
Bsu36I CC'TnA_GG
Cuts at: 2105 2105
Size: 7756
BtgI C'CryG_G
Cuts at: 513 1065 4511 513
Size: 552 3446 3758
Fragments arranged by size:
3758 3446 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7017
Fragments arranged by size:
7017 683 56
BtsI GCAGTG_nn'
Cuts at: 2096 4879 5272 6066 6086 2096
Size: 2783 393 794 20 3766
Fragments arranged by size:
3766 2783 794 393 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7756
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2902 2937 4815 4850 529
Size: 35 2338 35 1878 35 3435
Fragments arranged by size:
3435 2338 1878 35 35 35
DraI TTT'AAA
Cuts at: 3606 5060 3606
Size: 1454 6302
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6763 809
Size: 507 5447 1802
Fragments arranged by size:
5447 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4029 5203 6092
Size: 54 1425 50 275 18 2199 1174 889
Cuts at: 6092 7721 8
Size: 1629 43
Fragments arranged by size:
2199 1629 1425 1174 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5203 1537
Size: 275 3391 4090
Fragments arranged by size:
4090 3391 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2364 3688 3913 5686 1353
Size: 1011 1324 225 1773 3423
Fragments arranged by size:
3423 1773 1324 1011 225
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6343 6717 6863 387
Size: 1064 138 4754 374 146 1280
Fragments arranged by size:
4754 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5011 5011
Size: 7756
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2718 2985 3669 4002 4226 5819 6416
Size: 393 1086 267 684 333 224 1593 597
Cuts at: 6416 7203 7471 1239
Size: 787 268 1524
Fragments arranged by size:
1593 1524 1086 787 684 597 393 333
268 267 224
EcoO109I rG'GnC_Cy
Cuts at: 2448 3248 2448
Size: 800 6956
EcoRI G'AATT_C
Cuts at: 3755 5065 5443 3755
Size: 1310 378 6068
Fragments arranged by size:
6068 1310 378
FspI TGC'GCA
Cuts at: 2634 6262 2634
Size: 3628 4128
HaeII r_GCGC'y
Cuts at: 1678 6903 1678
Size: 5225 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4141 4173 4268 4300 4998
Size: 32 135 32 2569 32 95 32 698
Cuts at: 4998 5030 6397 6429 6471 6503 1373
Size: 32 1367 32 42 32 2626
Fragments arranged by size:
2626 2569 1367 698 135 95 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2208 2326 2871 3033 4933 5100
Size: 988 281 270 118 545 162 1900 167
Cuts at: 5100 5346 669
Size: 246 3079
Fragments arranged by size:
3079 1900 988 545 281 270 246 167
162 118
HindIII A'AGCT_T
Cuts at: 748 4909 748
Size: 4161 3595
HpaI GTT'AAC
Cuts at: 4933 5346 4933
Size: 413 7343
KpnI G_GTAC'C
Cuts at: 5081 5081
Size: 7756
MfeI C'AATT_G
Cuts at: 3222 5355 3222
Size: 2133 5623
MluI A'CGCG_T
Cuts at: 7632 7632
Size: 7756
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6870 7054 1305
Size: 5565 184 2007
Fragments arranged by size:
5565 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5934
Fragments arranged by size:
5934 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5714 6073 6232 518
Size: 648 4548 359 159 2042
Fragments arranged by size:
4548 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3215 5172 5849 6997 7242 1606
Size: 1609 1957 677 1148 245 2120
Fragments arranged by size:
2120 1957 1609 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 4511 513
Size: 552 3446 3758
Fragments arranged by size:
3758 3446 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7756
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7756
NotI GC'GGCC_GC
Cuts at: 5203 5203
Size: 7756
NspI r_CATG'y
Cuts at: 1132 1229 2343 3369 5114 6659 1132
Size: 97 1114 1026 1745 1545 2229
Fragments arranged by size:
2229 1745 1545 1114 1026 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7756
PciI A'CATG_T
Cuts at: 2339 3365 6655 2339
Size: 1026 3290 3440
Fragments arranged by size:
3440 3290 1026
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7756
PfoI T'CCnGG_A
Cuts at: 2801 2801
Size: 7756
PmeI GTTT'AAAC
Cuts at: 5060 5060
Size: 7756
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4408 4440 5825 5857 7338 7370 4408
Size: 32 1385 32 1481 32 4794
Fragments arranged by size:
4794 1481 1385 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2448 3248 2448
Size: 800 6956
PshAI GACnn'nnGTC
Cuts at: 1613 2213 2411 1613
Size: 600 198 6958
Fragments arranged by size:
6958 600 198
PsiI TTA'TAA
Cuts at: 5326 5326
Size: 7756
PstI C_TGCA'G
Cuts at: 830 4056 5108 830
Size: 3226 1052 3478
Fragments arranged by size:
3478 3226 1052
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7756
PvuII CAG'CTG
Cuts at: 3215 3215
Size: 7756
SacI G_AGCT'C
Cuts at: 5013 5013
Size: 7756
SalI G'TCGA_C
Cuts at: 1936 5098 1936
Size: 3162 4594
SbfI CC_TGCA'GG
Cuts at: 5108 5108
Size: 7756
ScaI AGT'ACT
Cuts at: 3328 6004 7716 3328
Size: 2676 1712 3368
Fragments arranged by size:
3368 2676 1712
SfcI C'TryA_G
Cuts at: 826 1045 4052 5104 6239 6920 7111 826
Size: 219 3007 1052 1135 681 191 1471
Fragments arranged by size:
3007 1471 1135 1052 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7756
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7756
SmaI CCC'GGG
Cuts at: 1872 5083 1872
Size: 3211 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3278 4259 5860 6761 7023 820
Size: 197 926 1335 981 1601 901 262 1553
Fragments arranged by size:
1601 1553 1335 981 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4616 5547 493
Size: 4123 931 2702
Fragments arranged by size:
4123 2702 931
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7756
SphI G_CATG'C
Cuts at: 1132 5114 1132
Size: 3982 3774
SspI AAT'ATT
Cuts at: 5 52 2768 2849 5680 5
Size: 47 2716 81 2831 2081
Fragments arranged by size:
2831 2716 2081 81 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7756
StyI C'CwwG_G
Cuts at: 513 1065 4511 5195 513
Size: 552 3446 684 3074
Fragments arranged by size:
3446 3074 684 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5783 5800 5953 6138 6583 325
Size: 1255 4203 17 153 185 445 1498
Fragments arranged by size:
4203 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2852 3326 3376
Size: 274 80 33 51 1443 875 474 50
Cuts at: 3376 4741 6002 7714 96
Size: 1365 1261 1712 138
Fragments arranged by size:
1712 1443 1365 1261 875 474 274 138
80 51 50 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7716 7748 7716
Size: 32 7724
Tth111I GACn'n_nGTC
Cuts at: 1316 4630 1316
Size: 3314 4442
XbaI T'CTAG_A
Cuts at: 2125 5092 2125
Size: 2967 4789
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3109 4107 3109
Size: 998 6758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7756
XmaI C'CCGG_G
Cuts at: 1870 5081 1870
Size: 3211 4545
XmnI GAAnn'nnTTC
Cuts at: 2358 4539 5001 5885 2358
Size: 2181 462 884 4229
Fragments arranged by size:
4229 2181 884 462
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5564 276
Size: 53 83 186 936 4030 2468
Fragments arranged by size:
4030 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI