Restriction map for
pFN21AA1700
(Circular) (Six-base) MAPSORT of: pFN21AA1700.seq Check: 6549 from: 1 to: 6700
pFN21AA1700 6700 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2360 2370 2360
Size: 10 6690
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4510 278
Size: 53 83 186 936 2974 2468
Fragments arranged by size:
2974 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4043 1937
Size: 2106 4594
Acc65I G'GTAC_C
Cuts at: 4021 4021
Size: 6700
AclI AA'CG_TT
Cuts at: 1563 4827 5200 1563
Size: 3264 373 3063
Fragments arranged by size:
3264 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2286 3417 4763 6147 6415 1632
Size: 654 1131 1346 1384 268 1917
Fragments arranged by size:
1917 1384 1346 1131 654 268
AflII C'TTAA_G
Cuts at: 820 1017 3611 820
Size: 197 2594 3909
Fragments arranged by size:
3909 2594 197
AflIII A'CryG_T
Cuts at: 1203 2796 5599 6576 1203
Size: 1593 2803 977 1327
Fragments arranged by size:
2803 1593 1327 977
AgeI A'CCGG_T
Cuts at: 5531 5531
Size: 6700
AhdI GACnn_n'nnGTC
Cuts at: 5429 5429
Size: 6700
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6700
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3470 3779 1184
Size: 2286 309 4105
Fragments arranged by size:
4105 2286 309
AlwNI CAG_nnn'CTG
Cuts at: 3672 3885 4443 6015 3672
Size: 213 558 1572 4357
Fragments arranged by size:
4357 1572 558 213
ApaLI G'TGCA_C
Cuts at: 2729 4757 5913 2729
Size: 2028 1156 3516
Fragments arranged by size:
3516 2028 1156
ApoI r'AATT_y
Cuts at: 1493 2487 2658 3896 4009 4235 4387 6496 1493
Size: 994 171 1238 113 226 152 2109 1697
Fragments arranged by size:
2109 1697 1238 994 226 171 152 113
AseI AT'TA_AT
Cuts at: 160 1032 2142 5254 160
Size: 872 1110 3112 1606
Fragments arranged by size:
3112 1606 1110 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6700
AvaI C'yCGr_G
Cuts at: 1870 1943 2609 4025 1870
Size: 73 666 1416 4545
Fragments arranged by size:
4545 1416 666 73
BamHI G'GATC_C
Cuts at: 4030 4397 4030
Size: 367 6333
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3482 4021 5476 618
Size: 325 217 627 1695 539 1455 1842
Fragments arranged by size:
1842 1695 1455 627 539 325 217
BanII G_rGCy'C
Cuts at: 1391 2208 3857 4019 1391
Size: 817 1649 162 4072
Fragments arranged by size:
4072 1649 817 162
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3183 928
Size: 971 1284 4445
Fragments arranged by size:
4445 1284 971
BbvCI CC'TCA_GC
Cuts at: 3712 3712
Size: 6700
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4891 4925 1903
Size: 34 2954 34 3678
Fragments arranged by size:
3678 2954 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4592 5802 4592
Size: 1210 5490
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6700
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3242 3615 5311
Size: 107 122 71 631 605 1570 373 1696
Cuts at: 5311 136
Size: 1525
Fragments arranged by size:
1696 1570 1525 631 605 373 122 107
71
BglII A'GATC_T
Cuts at: 6695 6695
Size: 6700
BlpI GC'TnA_GC
Cuts at: 4117 4117
Size: 6700
Bme1580I G_kGCm'C
Cuts at: 1790 2733 3087 3096 4761 5917 1790
Size: 943 354 9 1665 1156 2573
Fragments arranged by size:
2573 1665 1156 943 354 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6700
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4431 5389 442
Size: 416 53 83 399 24 3014 958 1753
Fragments arranged by size:
3014 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6700
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2543 2543
Size: 6700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2357 2931 2972 5360 1239
Size: 1118 574 41 2388 2579
Fragments arranged by size:
2579 2388 1118 574 41
Bpu10I CC'TnA_GC
Cuts at: 3712 3712
Size: 6700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2921 3176 4825 5690 5988 2921
Size: 255 1649 865 298 3633
Fragments arranged by size:
3633 1649 865 298 255
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3771 4000 5363 882
Size: 617 2272 229 1363 2219
Fragments arranged by size:
2272 2219 1363 617 229
BsaAI yAC'GTr
Cuts at: 493 1204 4491 493
Size: 711 3287 2702
Fragments arranged by size:
3287 2702 711
BsaBI GATnn'nnATC
Cuts at: 6694 6694
Size: 6700
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3008 4507 4889 5524
Size: 53 83 186 936 1475 1499 382 635
Cuts at: 5524 275
Size: 1451
Fragments arranged by size:
1499 1475 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5133 5531 5805 5952 6363 5133
Size: 398 274 147 411 5470
Fragments arranged by size:
5470 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2833 2863 3768 3798 1178
Size: 30 165 30 1430 30 905 30 4080
Fragments arranged by size:
4080 1430 905 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3256 1186
Size: 2070 4630
BseYI C'CCAG_C
Cuts at: 2741 3086 3095 3940 5903 6399 2741
Size: 345 9 845 1963 496 3042
Fragments arranged by size:
3042 1963 845 496 345 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2930 3122 3137 1163
Size: 573 1194 192 15 4726
Fragments arranged by size:
4726 1194 573 192 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4150 4911 5939 1540
Size: 275 186 2149 761 1028 2301
Fragments arranged by size:
2301 2149 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2208 2733 4761 4846 5917 2208
Size: 525 2028 85 1071 2991
Fragments arranged by size:
2991 2028 1071 525 85
BsmI GAATG_Cn'
Cuts at: 2215 3457 4211 4304 2215
Size: 1242 754 93 4611
Fragments arranged by size:
4611 1242 754 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2999 2999
Size: 6700
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2208 2733 3087 3096 3857 4019 4761
Size: 399 418 525 354 9 761 162 742
Cuts at: 4761 4846 5917 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 761 742 525 418 399 354
162 85 9
BspEI T'CCGG_A
Cuts at: 6363 6363
Size: 6700
BspHI T'CATG_A
Cuts at: 2781 4589 6319 2781
Size: 1808 1730 3162
Fragments arranged by size:
3162 1808 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2360 2370 2447 4055 844
Size: 1516 10 77 1608 3489
Fragments arranged by size:
3489 1608 1516 77 10
BsrBI CCG'CTC
Cuts at: 1394 1823 2754 4587 1394
Size: 429 931 1833 3507
Fragments arranged by size:
3507 1833 931 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5195 5369 65
Size: 1183 3947 174 1396
Fragments arranged by size:
3947 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2097 5344 5531 1700
Size: 110 287 3247 187 2869
Fragments arranged by size:
3247 2869 287 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6700
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6700
BssSI C'ACGA_G
Cuts at: 4760 5772 4760
Size: 1012 5688
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3885 794
Size: 3091 3609
BstBI TT'CG_AA
Cuts at: 5504 5504
Size: 6700
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6700
BstXI CCAn_nnnn'nTGG
Cuts at: 3310 3310
Size: 6700
BstYI r'GATC_y
Cuts at: 1972 2745 4030 4397 4782 4799 6251 6337 6349
Size: 773 1285 367 385 17 1452 86 12
Cuts at: 6349 6695 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1285 773 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2666 513
Size: 552 939 662 4547
Fragments arranged by size:
4547 939 662 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2749 487
Size: 683 56 1523 4438
Fragments arranged by size:
4438 1523 683 56
BtsI GCAGTG_nn'
Cuts at: 3484 3509 3736 4216 5010 5030 3484
Size: 25 227 480 794 20 5154
Fragments arranged by size:
5154 794 480 227 25 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6700
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6665
DraI TTT'AAA
Cuts at: 4004 4004
Size: 6700
DraIII CAC_nnn'GTG
Cuts at: 3026 3026
Size: 6700
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5707 809
Size: 507 4391 1802
Fragments arranged by size:
4391 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2099 4147 5036
Size: 54 1425 50 275 18 269 2048 889
Cuts at: 5036 6665 8
Size: 1629 43
Fragments arranged by size:
2048 1629 1425 889 275 269 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4147 1537
Size: 275 2335 4090
Fragments arranged by size:
4090 2335 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2305 2336 2780 3843 3930 4630 1353
Size: 952 31 444 1063 87 700 3423
Fragments arranged by size:
3423 1063 952 700 444 87 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2746 3850 5287 5661 5807 387
Size: 1064 138 1157 1104 1437 374 146 1280
Fragments arranged by size:
1437 1280 1157 1104 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2206 2206
Size: 6700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2286 2357 2931 2972 3417 4763 5360
Size: 393 654 71 574 41 445 1346 597
Cuts at: 5360 6147 6415 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1346 787 654 597 574 445 393
268 71 41
EcoNI CCTnn'n_nnAGG
Cuts at: 2531 2531
Size: 6700
EcoO109I rG'GnC_Cy
Cuts at: 3495 3495
Size: 6700
EcoRI G'AATT_C
Cuts at: 2487 4009 4387 2487
Size: 1522 378 4800
Fragments arranged by size:
4800 1522 378
EcoRV GAT'ATC
Cuts at: 3214 3631 3214
Size: 417 6283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2617 3217 2617
Size: 600 6100
FspI TGC'GCA
Cuts at: 3797 5206 3797
Size: 1409 5291
HaeII r_GCGC'y
Cuts at: 1678 3143 5847 1678
Size: 1465 2704 2531
Fragments arranged by size:
2704 2531 1465
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2011 2043 2261 2276 2293
Size: 32 135 32 439 32 218 15 17
Cuts at: 2293 2308 3203 3235 3768 3800 3894 3911 3926
Size: 15 895 32 533 32 94 17 15
Cuts at: 3926 3943 5341 5373 5415 5447 1373
Size: 17 1398 32 42 32 2626
Fragments arranged by size:
2626 1398 895 533 439 218 135 94
42 32 32 32 32 32 32 32
17 17 17 15 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2236 4044 4290 669
Size: 988 281 298 1808 246 3079
Fragments arranged by size:
3079 1808 988 298 281 246
HindIII A'AGCT_T
Cuts at: 748 2159 2811 748
Size: 1411 652 4637
Fragments arranged by size:
4637 1411 652
HpaI GTT'AAC
Cuts at: 4290 4290
Size: 6700
KpnI G_GTAC'C
Cuts at: 4025 4025
Size: 6700
MfeI C'AATT_G
Cuts at: 4299 4299
Size: 6700
MluI A'CGCG_T
Cuts at: 6576 6576
Size: 6700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2652 5814 5998 1305
Size: 1347 3162 184 2007
Fragments arranged by size:
3162 2007 1347 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4878
Fragments arranged by size:
4878 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3519 4658 5017 5176 518
Size: 648 2353 1139 359 159 2042
Fragments arranged by size:
2353 2042 1139 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3130 3672 3753 3763 4116 4793 5941 6186
Size: 1524 542 81 10 353 677 1148 245
Cuts at: 6186 1606
Size: 2120
Fragments arranged by size:
2120 1524 1148 677 542 353 245 81
10
NaeI GCC'GGC
Cuts at: 2099 2099
Size: 6700
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5209
Fragments arranged by size:
5209 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6700
NgoMIV G'CCGG_C
Cuts at: 2097 2097
Size: 6700
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6700
NotI GC'GGCC_GC
Cuts at: 4147 4147
Size: 6700
NspI r_CATG'y
Cuts at: 1132 1229 2800 4058 5603 1132
Size: 97 1571 1258 1545 2229
Fragments arranged by size:
2229 1571 1545 1258 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6700
PciI A'CATG_T
Cuts at: 2796 5599 2796
Size: 2803 3897
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2131 2878 3672 1108
Size: 1023 747 794 4136
Fragments arranged by size:
4136 1023 794 747
PfoI T'CCnGG_A
Cuts at: 2877 2877
Size: 6700
PmeI GTTT'AAAC
Cuts at: 4004 4004
Size: 6700
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4769 4801 6282 6314 4769
Size: 32 1481 32 5155
Fragments arranged by size:
5155 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3495 3495
Size: 6700
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6700
PsiI TTA'TAA
Cuts at: 4270 4270
Size: 6700
PstI C_TGCA'G
Cuts at: 830 3420 3758 4052 830
Size: 2590 338 294 3478
Fragments arranged by size:
3478 2590 338 294
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6700
PvuII CAG'CTG
Cuts at: 3672 3753 3763 3672
Size: 81 10 6609
Fragments arranged by size:
6609 81 10
SacI G_AGCT'C
Cuts at: 2208 2208
Size: 6700
SalI G'TCGA_C
Cuts at: 1936 4042 1936
Size: 2106 4594
SapI GCTCTTCn'nnn_
Cuts at: 2336 3930 2336
Size: 1594 5106
SbfI CC_TGCA'GG
Cuts at: 4052 4052
Size: 6700
ScaI AGT'ACT
Cuts at: 4948 6660 4948
Size: 1712 4988
SfcI C'TryA_G
Cuts at: 826 1045 3416 3748 3754 4048 5183 5864 6055
Size: 219 2371 332 6 294 1135 681 191
Cuts at: 6055 826
Size: 1471
Fragments arranged by size:
2371 1471 1135 681 332 294 219 191
6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6700
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6700
SmaI CCC'GGG
Cuts at: 1872 4027 1872
Size: 2155 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2936 3191 3611 4804 5705 5967
Size: 197 926 993 255 420 1193 901 262
Cuts at: 5967 820
Size: 1553
Fragments arranged by size:
1553 1193 993 926 901 420 262 255
197
SnaBI TAC'GTA
Cuts at: 493 4491 493
Size: 3998 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6700
SphI G_CATG'C
Cuts at: 1132 4058 1132
Size: 2926 3774
SspI AAT'ATT
Cuts at: 5 52 4624 5
Size: 47 4572 2081
Fragments arranged by size:
4572 2081 47
StuI AGG'CCT
Cuts at: 1517 3430 1517
Size: 1913 4787
StyI C'CwwG_G
Cuts at: 513 1065 2004 4139 513
Size: 552 939 2135 3074
Fragments arranged by size:
3074 2135 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3451 4727 4744 4897 5082 5527 325
Size: 1255 1871 1276 17 153 185 445 1498
Fragments arranged by size:
1871 1498 1276 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2320 4946 6658
Size: 274 80 33 51 1443 343 2626 1712
Cuts at: 6658 96
Size: 138
Fragments arranged by size:
2626 1712 1443 343 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6660 6692 6660
Size: 32 6668
Tth111I GACn'n_nGTC
Cuts at: 1316 3782 1316
Size: 2466 4234
XbaI T'CTAG_A
Cuts at: 4036 4036
Size: 6700
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2018 3896 2018
Size: 1878 4822
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6700
XmaI C'CCGG_G
Cuts at: 1870 4025 1870
Size: 2155 4545
XmnI GAAnn'nnTTC
Cuts at: 2345 4829 2345
Size: 2484 4216
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4508 276
Size: 53 83 186 936 2974 2468
Fragments arranged by size:
2974 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI