Restriction map for
pFN21AA1692
(Circular) (Six-base) MAPSORT of: pFN21AA1692.seq Check: 8858 from: 1 to: 7204
pFN21AA1692 7204 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3785 3785
Size: 7204
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5014 278
Size: 53 83 186 936 3478 2468
Fragments arranged by size:
3478 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4547 1937
Size: 2610 4594
Acc65I G'GTAC_C
Cuts at: 4525 4525
Size: 7204
AclI AA'CG_TT
Cuts at: 1563 5331 5704 1563
Size: 3768 373 3063
Fragments arranged by size:
3768 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2124 3507 3806 4057 5267 6651 6919 1632
Size: 492 1383 299 251 1210 1384 268 1917
Fragments arranged by size:
1917 1384 1383 1210 492 299 268 251
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7007
AflIII A'CryG_T
Cuts at: 1203 4062 6103 7080 1203
Size: 2859 2041 977 1327
Fragments arranged by size:
2859 2041 1327 977
AgeI A'CCGG_T
Cuts at: 6035 6035
Size: 7204
AhdI GACnn_n'nnGTC
Cuts at: 5933 5933
Size: 7204
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7204
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4114 1184
Size: 2930 4274
AlwNI CAG_nnn'CTG
Cuts at: 2925 3497 3575 3719 3883 4947 6519 2925
Size: 572 78 144 164 1064 1572 3610
Fragments arranged by size:
3610 1572 1064 572 164 144 78
ApaI G_GGCC'C
Cuts at: 3035 3035
Size: 7204
ApaLI G'TGCA_C
Cuts at: 5261 6417 5261
Size: 1156 6048
ApoI r'AATT_y
Cuts at: 1493 2408 3155 3562 4513 4739 4891 7000 1493
Size: 915 747 407 951 226 152 2109 1697
Fragments arranged by size:
2109 1697 951 915 747 407 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2592 5758 160
Size: 872 1560 3166 1606
Fragments arranged by size:
3166 1606 1560 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7204
AvaI C'yCGr_G
Cuts at: 1870 1943 2696 4359 4529 1870
Size: 73 753 1663 170 4545
Fragments arranged by size:
4545 1663 753 170 73
BamHI G'GATC_C
Cuts at: 3852 4534 4901 3852
Size: 682 367 6155
Fragments arranged by size:
6155 682 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4525 5980 618
Size: 325 217 627 2738 1455 1842
Fragments arranged by size:
2738 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2259 3035 3937 4523 1391
Size: 868 776 902 586 4072
Fragments arranged by size:
4072 902 868 776 586
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2097 2313 3210 3249 3513 3744 3953
Size: 971 198 216 897 39 264 231 209
Cuts at: 3953 928
Size: 4179
Fragments arranged by size:
4179 971 897 264 231 216 209 198
39
BbvCI CC'TCA_GC
Cuts at: 2583 3668 3843 2583
Size: 1085 175 5944
Fragments arranged by size:
5944 1085 175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5395 5429 1903
Size: 34 50 34 3374 34 3678
Fragments arranged by size:
3678 3374 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5096 6306 5096
Size: 1210 5994
BclI T'GATC_A
Cuts at: 1548 2497 3721 1548
Size: 949 1224 5031
Fragments arranged by size:
5031 1224 949
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 7204
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5815 136
Size: 107 122 71 631 605 4143 1525
Fragments arranged by size:
4143 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2041 2485 7199 2041
Size: 444 4714 2046
Fragments arranged by size:
4714 2046 444
BlpI GC'TnA_GC
Cuts at: 4621 4621
Size: 7204
Bme1580I G_kGCm'C
Cuts at: 1790 3035 5265 6421 1790
Size: 1245 2230 1156 2573
Fragments arranged by size:
2573 2230 1245 1156
BmgBI CAC'GTC
Cuts at: 1318 4063 1318
Size: 2745 4459
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2369 4935 5893
Size: 416 53 83 399 24 952 2566 958
Cuts at: 5893 442
Size: 1753
Fragments arranged by size:
2566 1753 958 952 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7204
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3011 3588 4421 5864 1239
Size: 1772 577 833 1443 2579
Fragments arranged by size:
2579 1772 1443 833 577
Bpu10I CC'TnA_GC
Cuts at: 2583 3668 3843 2583
Size: 1085 175 5944
Fragments arranged by size:
5944 1085 175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5329 6194 6492 5329
Size: 865 298 6041
Fragments arranged by size:
6041 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2210 2281 5867 882
Size: 617 711 71 3586 2219
Fragments arranged by size:
3586 2219 711 617 71
BsaAI yAC'GTr
Cuts at: 493 1204 4995 493
Size: 711 3791 2702
Fragments arranged by size:
3791 2702 711
BsaBI GATnn'nnATC
Cuts at: 3857 7198 3857
Size: 3341 3863
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5011 5393 6028 275
Size: 53 83 186 936 3478 382 635 1451
Fragments arranged by size:
3478 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5637 6035 6309 6456 6867 5637
Size: 398 274 147 411 5974
Fragments arranged by size:
5974 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2051 2081 2954 2984 3002
Size: 30 165 30 648 30 873 30 18
Cuts at: 3002 3032 3983 4013 1178
Size: 30 951 30 4369
Fragments arranged by size:
4369 951 873 648 165 30 30 30
30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2304 3010 1186
Size: 1118 706 5380
Fragments arranged by size:
5380 1118 706
BseYI C'CCAG_C
Cuts at: 2054 3511 6407 6903 2054
Size: 1457 2896 496 2355
Fragments arranged by size:
2896 2355 1457 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2458 1163
Size: 573 722 5909
Fragments arranged by size:
5909 722 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4654 5415 6443 1540
Size: 275 186 2653 761 1028 2301
Fragments arranged by size:
2653 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3937 3967 5265 5350 6421 3937
Size: 30 1298 85 1071 4720
Fragments arranged by size:
4720 1298 1071 85 30
BsmI GAATG_Cn'
Cuts at: 2965 4426 4715 4808 2965
Size: 1461 289 93 5361
Fragments arranged by size:
5361 1461 289 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2259 3035 3937 3967 4523 5265 5350
Size: 399 469 776 902 30 556 742 85
Cuts at: 5350 6421 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 902 776 742 556 469 399
85 30
BspEI T'CCGG_A
Cuts at: 6867 6867
Size: 7204
BspHI T'CATG_A
Cuts at: 2340 5093 6823 2340
Size: 2753 1730 2721
Fragments arranged by size:
2753 2721 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2569 3785 4559 844
Size: 1725 1216 774 3489
Fragments arranged by size:
3489 1725 1216 774
BsrBI CCG'CTC
Cuts at: 1394 1823 4350 5091 1394
Size: 429 2527 741 3507
Fragments arranged by size:
3507 2527 741 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2397 3078 4103 5699 5873 65
Size: 1183 1149 681 1025 1596 174 1396
Fragments arranged by size:
1596 1396 1183 1149 1025 681 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5848 6035 1700
Size: 110 4038 187 2869
Fragments arranged by size:
4038 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7204
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7204
BssSI C'ACGA_G
Cuts at: 2049 5264 6276 2049
Size: 3215 1012 2977
Fragments arranged by size:
3215 2977 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3962 794
Size: 3168 4036
BstBI TT'CG_AA
Cuts at: 4368 6008 4368
Size: 1640 5564
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7204
BstXI CCAn_nnnn'nTGG
Cuts at: 4501 4501
Size: 7204
BstYI r'GATC_y
Cuts at: 1972 2041 2485 3852 4015 4534 4901 5286 5303
Size: 69 444 1367 163 519 367 385 17
Cuts at: 5303 6755 6841 6853 7199 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1367 519 444 385 367 346
163 86 69 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3690 3848 513
Size: 552 2625 158 3869
Fragments arranged by size:
3869 2625 552 158
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6465
Fragments arranged by size:
6465 683 56
BtsI GCAGTG_nn'
Cuts at: 2409 4720 5514 5534 2409
Size: 2311 794 20 4079
Fragments arranged by size:
4079 2311 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7204
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3363 3398 529
Size: 35 2799 35 4335
Fragments arranged by size:
4335 2799 35 35
DraI TTT'AAA
Cuts at: 4508 4508
Size: 7204
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6211 809
Size: 507 4895 1802
Fragments arranged by size:
4895 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2952 3460 4147
Size: 54 1425 50 275 18 1122 508 687
Cuts at: 4147 4329 4651 5540 7169 8
Size: 182 322 889 1629 43
Fragments arranged by size:
1629 1425 1122 889 687 508 322 275
182 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4651 1537
Size: 275 2839 4090
Fragments arranged by size:
4090 2839 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2069 2904 5134 1353
Size: 716 835 2230 3423
Fragments arranged by size:
3423 2230 835 716
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5791 6165 6311 387
Size: 1064 138 4202 374 146 1280
Fragments arranged by size:
4202 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3935 3935
Size: 7204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 3011 3507 3588 3806 4057 4421
Size: 393 492 887 496 81 218 251 364
Cuts at: 4421 5267 5864 6651 6919 1239
Size: 846 597 787 268 1524
Fragments arranged by size:
1524 887 846 787 597 496 492 393
364 268 251 218 81
EcoO109I rG'GnC_Cy
Cuts at: 2059 2245 3032 2059
Size: 186 787 6231
Fragments arranged by size:
6231 787 186
EcoRI G'AATT_C
Cuts at: 3155 4513 4891 3155
Size: 1358 378 5468
Fragments arranged by size:
5468 1358 378
EcoRV GAT'ATC
Cuts at: 2338 2338
Size: 7204
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2114 3372 3664 3787 4187 2114
Size: 1258 292 123 400 5131
Fragments arranged by size:
5131 1258 400 292 123
FspI TGC'GCA
Cuts at: 5710 5710
Size: 7204
HaeII r_GCGC'y
Cuts at: 1678 6351 1678
Size: 4673 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 2207 2239 3002
Size: 32 135 32 479 32 124 32 763
Cuts at: 3002 3034 3551 3583 5845 5877 5919 5951 1373
Size: 32 517 32 2262 32 42 32 2626
Fragments arranged by size:
2626 2262 763 517 479 135 124 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4548 4794 669
Size: 988 281 2610 246 3079
Fragments arranged by size:
3079 2610 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2106 748
Size: 1358 5846
HpaI GTT'AAC
Cuts at: 4794 4794
Size: 7204
KpnI G_GTAC'C
Cuts at: 4529 4529
Size: 7204
MfeI C'AATT_G
Cuts at: 4803 4803
Size: 7204
MluI A'CGCG_T
Cuts at: 7080 7080
Size: 7204
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6318 6502 1305
Size: 5013 184 2007
Fragments arranged by size:
5013 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2954 3462 4149 4331 10
Size: 54 1425 343 1122 508 687 182 2883
Fragments arranged by size:
2883 1425 1122 687 508 343 182 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2402 2904 4096 4448 5162 5521 5680
Size: 648 1236 502 1192 352 714 359 159
Cuts at: 5680 518
Size: 2042
Fragments arranged by size:
2042 1236 1192 714 648 502 359 352
159
MspA1I CmG'CkG
Cuts at: 1606 2632 3511 4620 5297 6445 6690 1606
Size: 1026 879 1109 677 1148 245 2120
Fragments arranged by size:
2120 1148 1109 1026 879 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3690 3848 513
Size: 552 2625 158 3869
Fragments arranged by size:
3869 2625 552 158
NdeI CA'TA_TG
Cuts at: 387 3685 387
Size: 3298 3906
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7204
NotI GC'GGCC_GC
Cuts at: 4651 4651
Size: 7204
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 7204
NspI r_CATG'y
Cuts at: 1132 1229 2240 2675 2903 4562 6107 1132
Size: 97 1011 435 228 1659 1545 2229
Fragments arranged by size:
2229 1659 1545 1011 435 228 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7204
PciI A'CATG_T
Cuts at: 6103 6103
Size: 7204
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7204
PmeI GTTT'AAAC
Cuts at: 4508 4508
Size: 7204
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5273 5305 6786 6818 5273
Size: 32 1481 32 5659
Fragments arranged by size:
5659 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 2059
Size: 7204
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7204
PsiI TTA'TAA
Cuts at: 4774 4774
Size: 7204
PspOMI G'GGCC_C
Cuts at: 3031 3031
Size: 7204
PspXI vC'TCGA_Gb
Cuts at: 2696 2696
Size: 7204
PstI C_TGCA'G
Cuts at: 830 4556 830
Size: 3726 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7204
PvuII CAG'CTG
Cuts at: 2632 3511 2632
Size: 879 6325
SacI G_AGCT'C
Cuts at: 3937 3937
Size: 7204
SalI G'TCGA_C
Cuts at: 1936 4546 1936
Size: 2610 4594
SbfI CC_TGCA'GG
Cuts at: 4556 4556
Size: 7204
ScaI AGT'ACT
Cuts at: 5452 7164 5452
Size: 1712 5492
SexAI A'CCwGG_T
Cuts at: 2937 4023 2937
Size: 1086 6118
SfcI C'TryA_G
Cuts at: 826 1045 3624 4552 5687 6368 6559 826
Size: 219 2579 928 1135 681 191 1471
Fragments arranged by size:
2579 1471 1135 928 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7204
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7204
SmaI CCC'GGG
Cuts at: 1872 4361 4531 1872
Size: 2489 170 4545
Fragments arranged by size:
4545 2489 170
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2696 5308 6209 6471 820
Size: 197 926 753 2612 901 262 1553
Fragments arranged by size:
2612 1553 926 901 753 262 197
SnaBI TAC'GTA
Cuts at: 493 4995 493
Size: 4502 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7204
SphI G_CATG'C
Cuts at: 1132 2240 4562 1132
Size: 1108 2322 3774
Fragments arranged by size:
3774 2322 1108
SspI AAT'ATT
Cuts at: 5 52 3541 5128 5
Size: 47 3489 1587 2081
Fragments arranged by size:
3489 2081 1587 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7204
StyI C'CwwG_G
Cuts at: 513 1065 2478 2530 3114 3463 3690 3848 4407
Size: 552 1413 52 584 349 227 158 559
Cuts at: 4407 4643 513
Size: 236 3074
Fragments arranged by size:
3074 1413 584 559 552 349 236 227
158 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2234 5231 5248 5401 5586 6031 325
Size: 1255 654 2997 17 153 185 445 1498
Fragments arranged by size:
2997 1498 1255 654 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4309 5450 7162
Size: 274 80 33 51 1443 2332 1141 1712
Cuts at: 7162 96
Size: 138
Fragments arranged by size:
2332 1712 1443 1141 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7164 7196 7164
Size: 32 7172
Tth111I GACn'n_nGTC
Cuts at: 1316 2221 1316
Size: 905 6299
XbaI T'CTAG_A
Cuts at: 4540 4540
Size: 7204
XhoI C'TCGA_G
Cuts at: 1943 2696 1943
Size: 753 6451
XmaI C'CCGG_G
Cuts at: 1870 4359 4529 1870
Size: 2489 170 4545
Fragments arranged by size:
4545 2489 170
XmnI GAAnn'nnTTC
Cuts at: 5333 5333
Size: 7204
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5012 276
Size: 53 83 186 936 3478 2468
Fragments arranged by size:
3478 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PsrI
RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI