Restriction map for
pFN21AA1687
(Circular) (Six-base) MAPSORT of: pFN21AA1687.seq Check: 9166 from: 1 to: 6859
pFN21AA1687 6859 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4669 278
Size: 53 83 186 936 3133 2468
Fragments arranged by size:
3133 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4202 1937
Size: 2265 4594
Acc65I G'GTAC_C
Cuts at: 4180 4180
Size: 6859
AclI AA'CG_TT
Cuts at: 1563 4986 5359 1563
Size: 3423 373 3063
Fragments arranged by size:
3423 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2210 2466 2490 4922 6306 6574 1632
Size: 464 114 256 24 2432 1384 268 1917
Fragments arranged by size:
2432 1917 1384 464 268 256 114 24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6662
AflIII A'CryG_T
Cuts at: 1203 5758 6735 1203
Size: 4555 977 1327
Fragments arranged by size:
4555 1327 977
AgeI A'CCGG_T
Cuts at: 5690 5690
Size: 6859
AhdI GACnn_n'nnGTC
Cuts at: 5588 5588
Size: 6859
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6859
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6859
AlwNI CAG_nnn'CTG
Cuts at: 2015 2615 3145 4602 6174 2015
Size: 600 530 1457 1572 2700
Fragments arranged by size:
2700 1572 1457 600 530
ApaLI G'TGCA_C
Cuts at: 4916 6072 4916
Size: 1156 5703
ApoI r'AATT_y
Cuts at: 1493 2272 2344 2704 2960 2985 4168 4394 4546
Size: 779 72 360 256 25 1183 226 152
Cuts at: 4546 6655 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1183 779 360 256 226 152
72 25
AseI AT'TA_AT
Cuts at: 160 1032 3015 5413 160
Size: 872 1983 2398 1606
Fragments arranged by size:
2398 1983 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6859
AvaI C'yCGr_G
Cuts at: 1870 1943 2172 3981 4184 1870
Size: 73 229 1809 203 4545
Fragments arranged by size:
4545 1809 229 203 73
BamHI G'GATC_C
Cuts at: 4189 4556 4189
Size: 367 6492
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2227 3383 3964 4180 5635
Size: 325 217 627 440 1156 581 216 1455
Cuts at: 5635 618
Size: 1842
Fragments arranged by size:
1842 1455 1156 627 581 440 325 217
216
BanII G_rGCy'C
Cuts at: 1391 4178 1391
Size: 2787 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2102 2454 2777 928
Size: 971 203 352 323 5010
Fragments arranged by size:
5010 971 352 323 203
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2492 2526 3242 3276 5050 5084 1903
Size: 34 555 34 716 34 1774 34 3678
Fragments arranged by size:
3678 1774 716 555 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4751 5961 4751
Size: 1210 5649
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6859
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5470 136
Size: 107 122 71 631 605 3798 1525
Fragments arranged by size:
3798 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2465 3151 6854 2465
Size: 686 3703 2470
Fragments arranged by size:
3703 2470 686
BlpI GC'TnA_GC
Cuts at: 4276 4276
Size: 6859
Bme1580I G_kGCm'C
Cuts at: 1790 3295 4004 4920 6076 1790
Size: 1505 709 916 1156 2573
Fragments arranged by size:
2573 1505 1156 916 709
BmgBI CAC'GTC
Cuts at: 1318 3737 3824 1318
Size: 2419 87 4353
Fragments arranged by size:
4353 2419 87
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3104 4590 5548
Size: 416 53 83 399 24 1687 1486 958
Cuts at: 5548 442
Size: 1753
Fragments arranged by size:
1753 1687 1486 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6859
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2187 3996 4034 2187
Size: 1809 38 5012
Fragments arranged by size:
5012 1809 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2080 3889 5519 1239
Size: 841 1809 1630 2579
Fragments arranged by size:
2579 1809 1630 841
Bpu10I CC'TnA_GC
Cuts at: 2857 3550 2857
Size: 693 6166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3206 4984 5849 6147 3206
Size: 1778 865 298 3918
Fragments arranged by size:
3918 1778 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3026 5522 882
Size: 617 1527 2496 2219
Fragments arranged by size:
2496 2219 1527 617
BsaAI yAC'GTr
Cuts at: 493 1204 3661 4024 4650 493
Size: 711 2457 363 626 2702
Fragments arranged by size:
2702 2457 711 626 363
BsaBI GATnn'nnATC
Cuts at: 6853 6853
Size: 6859
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4666 5048 5683 275
Size: 53 83 186 936 3133 382 635 1451
Fragments arranged by size:
3133 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5292 5690 5964 6111 6522 5292
Size: 398 274 147 411 5629
Fragments arranged by size:
5629 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3405 3435 3787 3817 3887
Size: 30 165 30 2002 30 352 30 70
Cuts at: 3887 3917 1178
Size: 30 4120
Fragments arranged by size:
4120 2002 352 165 70 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6859
BseYI C'CCAG_C
Cuts at: 2584 6062 6558 2584
Size: 3478 496 2885
Fragments arranged by size:
3478 2885 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2735 3022 3095 1163
Size: 573 999 287 73 4927
Fragments arranged by size:
4927 999 573 287 73
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4309 5070 6098 1540
Size: 275 186 2308 761 1028 2301
Fragments arranged by size:
2308 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3808 4920 5005 6076 3808
Size: 1112 85 1071 4591
Fragments arranged by size:
4591 1112 1071 85
BsmI GAATG_Cn'
Cuts at: 2461 4370 4463 2461
Size: 1909 93 4857
Fragments arranged by size:
4857 1909 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3450 3450
Size: 6859
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3295 3808 4004 4178 4920 5005 6076
Size: 399 1505 513 196 174 742 85 1071
Cuts at: 6076 1391
Size: 2174
Fragments arranged by size:
2174 1505 1071 742 513 399 196 174
85
BspEI T'CCGG_A
Cuts at: 6522 6522
Size: 6859
BspHI T'CATG_A
Cuts at: 3595 4748 6478 3595
Size: 1153 1730 3976
Fragments arranged by size:
3976 1730 1153
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2145 4214 844
Size: 1301 2069 3489
Fragments arranged by size:
3489 2069 1301
BsrBI CCG'CTC
Cuts at: 1394 1823 4746 1394
Size: 429 2923 3507
Fragments arranged by size:
3507 2923 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2637 3898 5354 5528 65
Size: 1183 1389 1261 1456 174 1396
Fragments arranged by size:
1456 1396 1389 1261 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2242 5503 5690 1700
Size: 110 432 3261 187 2869
Fragments arranged by size:
3261 2869 432 187 110
BsrGI T'GTAC_A
Cuts at: 96 2891 96
Size: 2795 4064
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6859
BssSI C'ACGA_G
Cuts at: 4919 5931 4919
Size: 1012 5847
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3073 3299 794
Size: 2279 226 4354
Fragments arranged by size:
4354 2279 226
BstBI TT'CG_AA
Cuts at: 5663 5663
Size: 6859
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6859
BstYI r'GATC_y
Cuts at: 1972 2043 2465 2689 3151 3242 4189 4556 4941
Size: 71 422 224 462 91 947 367 385
Cuts at: 4941 4958 6410 6496 6508 6854 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 947 462 422 385 367 346
224 91 86 71 17 12
Bsu36I CC'TnA_GG
Cuts at: 2048 3356 3639 2048
Size: 1308 283 5268
Fragments arranged by size:
5268 1308 283
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6120
Fragments arranged by size:
6120 683 56
BtsI GCAGTG_nn'
Cuts at: 2616 3010 3062 4375 5169 5189 2616
Size: 394 52 1313 794 20 4286
Fragments arranged by size:
4286 1313 794 394 52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6859
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3805 3840 529
Size: 35 3241 35 3548
Fragments arranged by size:
3548 3241 35 35
DraI TTT'AAA
Cuts at: 3465 4163 3465
Size: 698 6161
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5866 809
Size: 507 4550 1802
Fragments arranged by size:
4550 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3394 4306 5195
Size: 54 1425 50 275 18 1564 912 889
Cuts at: 5195 6824 8
Size: 1629 43
Fragments arranged by size:
1629 1564 1425 912 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4306 1537
Size: 275 2494 4090
Fragments arranged by size:
4090 2494 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2156 2466 2514 2661 3024 3448 4117 4789
Size: 803 310 48 147 363 424 669 672
Cuts at: 4789 1353
Size: 3423
Fragments arranged by size:
3423 803 672 669 424 363 310 147
48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5446 5820 5966 387
Size: 1064 138 3857 374 146 1280
Fragments arranged by size:
3857 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2080 2096 2210 2466 2490 3889 4922
Size: 393 448 16 114 256 24 1399 1033
Cuts at: 4922 5519 6306 6574 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1399 1033 787 597 448 393 268
256 114 24 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2082 2082
Size: 6859
EcoO109I rG'GnC_Cy
Cuts at: 2139 3634 2139
Size: 1495 5364
EcoRI G'AATT_C
Cuts at: 2960 4168 4546 2960
Size: 1208 378 5273
Fragments arranged by size:
5273 1208 378
FspI TGC'GCA
Cuts at: 5365 5365
Size: 6859
HaeII r_GCGC'y
Cuts at: 1678 6006 1678
Size: 4328 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2801 2833 3227 3259 3938
Size: 32 135 32 1229 32 394 32 679
Cuts at: 3938 3970 5500 5532 5574 5606 1373
Size: 32 1530 32 42 32 2626
Fragments arranged by size:
2626 1530 1229 679 394 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3534 3675 3957 4114 4203 4449
Size: 988 281 1596 141 282 157 89 246
Cuts at: 4449 669
Size: 3079
Fragments arranged by size:
3079 1596 988 282 281 246 157 141
89
HindIII A'AGCT_T
Cuts at: 748 2358 748
Size: 1610 5249
HpaI GTT'AAC
Cuts at: 4114 4449 4114
Size: 335 6524
KpnI G_GTAC'C
Cuts at: 4184 4184
Size: 6859
MfeI C'AATT_G
Cuts at: 2741 4458 2741
Size: 1717 5142
MluI A'CGCG_T
Cuts at: 6735 6735
Size: 6859
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3918 4009 5973 6157 1305
Size: 2613 91 1964 184 2007
Fragments arranged by size:
2613 2007 1964 184 91
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5037
Fragments arranged by size:
5037 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3017 4817 5176 5335 518
Size: 648 843 1008 1800 359 159 2042
Fragments arranged by size:
2042 1800 1008 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2794 4275 4952 6100 6345 1606
Size: 1188 1481 677 1148 245 2120
Fragments arranged by size:
2120 1481 1188 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6307
NdeI CA'TA_TG
Cuts at: 387 4072 387
Size: 3685 3174
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6859
NotI GC'GGCC_GC
Cuts at: 4306 4306
Size: 6859
NspI r_CATG'y
Cuts at: 1132 1229 4137 4217 5762 1132
Size: 97 2908 80 1545 2229
Fragments arranged by size:
2908 2229 1545 97 80
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6859
PciI A'CATG_T
Cuts at: 5758 5758
Size: 6859
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2596 3369 1108
Size: 1488 773 4598
Fragments arranged by size:
4598 1488 773
PmeI GTTT'AAAC
Cuts at: 4163 4163
Size: 6859
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4928 4960 6441 6473 4928
Size: 32 1481 32 5314
Fragments arranged by size:
5314 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2139 3634 2139
Size: 1495 5364
PshAI GACnn'nnGTC
Cuts at: 1613 2801 1613
Size: 1188 5671
PsiI TTA'TAA
Cuts at: 2489 4429 2489
Size: 1940 4919
PstI C_TGCA'G
Cuts at: 830 2779 2793 4211 830
Size: 1949 14 1418 3478
Fragments arranged by size:
3478 1949 1418 14
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6859
PvuII CAG'CTG
Cuts at: 2794 2794
Size: 6859
SalI G'TCGA_C
Cuts at: 1936 4201 1936
Size: 2265 4594
SanDI GG'GwC_CC
Cuts at: 2139 3634 2139
Size: 1495 5364
SapI GCTCTTCn'nnn_
Cuts at: 2156 2466 4117 2156
Size: 310 1651 4898
Fragments arranged by size:
4898 1651 310
SbfI CC_TGCA'GG
Cuts at: 4211 4211
Size: 6859
ScaI AGT'ACT
Cuts at: 5107 6819 5107
Size: 1712 5147
SfcI C'TryA_G
Cuts at: 826 1045 2418 2775 2789 3303 3585 3621 4207
Size: 219 1373 357 14 514 282 36 586
Cuts at: 4207 5342 6023 6214 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1373 1135 681 586 514 357 282
219 191 36 14
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6859
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6859
SmaI CCC'GGG
Cuts at: 1872 4186 1872
Size: 2314 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3185 3981 4963 5864 6126 820
Size: 197 926 1242 796 982 901 262 1553
Fragments arranged by size:
1553 1242 982 926 901 796 262 197
SnaBI TAC'GTA
Cuts at: 493 3661 4650 493
Size: 3168 989 2702
Fragments arranged by size:
3168 2702 989
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6859
SphI G_CATG'C
Cuts at: 1132 4217 1132
Size: 3085 3774
SspI AAT'ATT
Cuts at: 5 52 4783 5
Size: 47 4731 2081
Fragments arranged by size:
4731 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6859
StyI C'CwwG_G
Cuts at: 513 1065 2709 2884 4298 513
Size: 552 1644 175 1414 3074
Fragments arranged by size:
3074 1644 1414 552 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3049 4886 4903 5056 5241 5686 325
Size: 1255 1469 1837 17 153 185 445 1498
Fragments arranged by size:
1837 1498 1469 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2891 5105 6817
Size: 274 80 33 51 1443 914 2214 1712
Cuts at: 6817 96
Size: 138
Fragments arranged by size:
2214 1712 1443 914 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3787 3819 6819 6851 3787
Size: 32 3000 32 3795
Fragments arranged by size:
3795 3000 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6859
XbaI T'CTAG_A
Cuts at: 4195 4195
Size: 6859
XhoI C'TCGA_G
Cuts at: 1943 3981 1943
Size: 2038 4821
XmaI C'CCGG_G
Cuts at: 1870 4184 1870
Size: 2314 4545
XmnI GAAnn'nnTTC
Cuts at: 2348 4988 2348
Size: 2640 4219
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4667 276
Size: 53 83 186 936 3133 2468
Fragments arranged by size:
3133 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI