Restriction map for
pFN21AA1685
(Circular) (Six-base) MAPSORT of: pFN21AA1685.seq Check: 8602 from: 1 to: 9010
pFN21AA1685 9010 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2382 3052 4360 4741 2382
Size: 670 1308 381 6651
Fragments arranged by size:
6651 1308 670 381
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6820 278
Size: 53 83 186 936 5284 2468
Fragments arranged by size:
5284 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6353 1937
Size: 4416 4594
Acc65I G'GTAC_C
Cuts at: 6331 6331
Size: 9010
AclI AA'CG_TT
Cuts at: 1563 7137 7510 1563
Size: 5574 373 3063
Fragments arranged by size:
5574 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2988 3369 4593 5096 5189 5463 5493 7073
Size: 1356 381 1224 503 93 274 30 1580
Cuts at: 7073 8457 8725 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1580 1384 1356 1224 503 381 274
268 93 30
AfeI AGC'GCT
Cuts at: 4980 4980
Size: 9010
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8813
AflIII A'CryG_T
Cuts at: 1203 3760 7909 8886 1203
Size: 2557 4149 977 1327
Fragments arranged by size:
4149 2557 1327 977
AgeI A'CCGG_T
Cuts at: 7841 7841
Size: 9010
AhdI GACnn_n'nnGTC
Cuts at: 7739 7739
Size: 9010
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9010
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5090 1184
Size: 3906 5104
AlwNI CAG_nnn'CTG
Cuts at: 2040 4025 4046 4082 4205 4517 4646 4733 5062
Size: 1985 21 36 123 312 129 87 329
Cuts at: 5062 6092 6753 8325 2040
Size: 1030 661 1572 2725
Fragments arranged by size:
2725 1985 1572 1030 661 329 312 129
123 87 36 21
ApaI G_GGCC'C
Cuts at: 4184 4476 4184
Size: 292 8718
ApaLI G'TGCA_C
Cuts at: 7067 8223 7067
Size: 1156 7854
ApoI r'AATT_y
Cuts at: 1493 2103 2993 3069 4902 6319 6545 6697 8806
Size: 610 890 76 1833 1417 226 152 2109
Cuts at: 8806 1493
Size: 1697
Fragments arranged by size:
2109 1833 1697 1417 890 610 226 152
76
AseI AT'TA_AT
Cuts at: 160 1032 7564 160
Size: 872 6532 1606
Fragments arranged by size:
6532 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9010
AvaI C'yCGr_G
Cuts at: 1870 1943 4753 5861 6335 1870
Size: 73 2810 1108 474 4545
Fragments arranged by size:
4545 2810 1108 474 73
BamHI G'GATC_C
Cuts at: 6340 6707 6340
Size: 367 8643
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4532 6331 7786 618
Size: 325 217 627 2745 1799 1455 1842
Fragments arranged by size:
2745 1842 1799 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3393 3692 3925 4184 4476 4575 5408 5601
Size: 2002 299 233 259 292 99 833 193
Cuts at: 5601 6329 1391
Size: 728 4072
Fragments arranged by size:
4072 2002 833 728 299 292 259 233
193 99
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3705 5304 928
Size: 971 1806 1599 4634
Fragments arranged by size:
4634 1806 1599 971
BbvCI CC'TCA_GC
Cuts at: 3403 5135 3403
Size: 1732 7278
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4744 4778 7201 7235 1903
Size: 34 2807 34 2423 34 3678
Fragments arranged by size:
3678 2807 2423 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4850 6902 8112 4850
Size: 2052 1210 5748
Fragments arranged by size:
5748 2052 1210
BclI T'GATC_A
Cuts at: 1548 2862 3682 5994 6225 1548
Size: 1314 820 2312 231 4333
Fragments arranged by size:
4333 2312 1314 820 231
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4448 7621 136
Size: 107 122 71 631 605 2776 3173 1525
Fragments arranged by size:
3173 2776 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2759 9005 2759
Size: 6246 2764
BlpI GC'TnA_GC
Cuts at: 2977 3425 5680 6427 2977
Size: 448 2255 747 5560
Fragments arranged by size:
5560 2255 747 448
Bme1580I G_kGCm'C
Cuts at: 1790 3452 4184 4476 5373 6191 7071 8227 1790
Size: 1662 732 292 897 818 880 1156 2573
Fragments arranged by size:
2573 1662 1156 897 880 818 732 292
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9010
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3186 3280 4255
Size: 416 53 83 399 24 1769 94 975
Cuts at: 4255 4331 4470 6741 7699 442
Size: 76 139 2271 958 1753
Fragments arranged by size:
2271 1769 1753 975 958 416 399 139
94 83 76 53 24
BmtI G_CTAG'C
Cuts at: 1056 5688 6114 1056
Size: 4632 426 3952
Fragments arranged by size:
4632 3952 426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2179 2677 2913 3859 4332 5128 7670 1239
Size: 940 498 236 946 473 796 2542 2579
Fragments arranged by size:
2579 2542 946 940 796 498 473 236
Bpu10I CC'TnA_GC
Cuts at: 3155 3403 5127 5135 3155
Size: 248 1724 8 7030
Fragments arranged by size:
7030 1724 248 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3686 3845 7135 8000 8298 3686
Size: 159 3290 865 298 4398
Fragments arranged by size:
4398 3290 865 298 159
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3805 3877 3916 4262 7673 882
Size: 617 2306 72 39 346 3411 2219
Fragments arranged by size:
3411 2306 2219 617 346 72 39
BsaAI yAC'GTr
Cuts at: 493 1204 3761 6801 493
Size: 711 2557 3040 2702
Fragments arranged by size:
3040 2702 2557 711
BsaBI GATnn'nnATC
Cuts at: 3462 9004 3462
Size: 5542 3468
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6817 7199 7834 275
Size: 53 83 186 936 5284 382 635 1451
Fragments arranged by size:
5284 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2824 7443 7841 8115 8262 8673 2824
Size: 4619 398 274 147 411 3161
Fragments arranged by size:
4619 3161 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2177 2207 2675 2705 3172
Size: 30 165 30 774 30 468 30 467
Cuts at: 3172 3202 1178
Size: 30 6986
Fragments arranged by size:
6986 774 468 467 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2148 2560 4152 5609 1186
Size: 962 412 1592 1457 4587
Fragments arranged by size:
4587 1592 1457 962 412
BseYI C'CCAG_C
Cuts at: 3217 3744 5062 5326 6128 8213 8709 3217
Size: 527 1318 264 802 2085 496 3518
Fragments arranged by size:
3518 2085 1318 802 527 496 264
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3383 4572 1163
Size: 573 1647 1189 5601
Fragments arranged by size:
5601 1647 1189 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5401 6460 7221 8249 1540
Size: 275 186 3400 1059 761 1028 2301
Fragments arranged by size:
3400 2301 1059 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2860 4318 4575 4760 7071 7156 8227 2860
Size: 1458 257 185 2311 85 1071 3643
Fragments arranged by size:
3643 2311 1458 1071 257 185 85
BsmI GAATG_Cn'
Cuts at: 2179 3432 6521 6614 2179
Size: 1253 3089 93 4575
Fragments arranged by size:
4575 3089 1253 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6295 6295
Size: 9010
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2860 3393 3452 3692 3925 4184 4318
Size: 399 1070 533 59 240 233 259 134
Cuts at: 4318 4476 4575 4760 5373 5408 5601 6191 6329
Size: 158 99 185 613 35 193 590 138
Cuts at: 6329 7071 7156 8227 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1070 742 613 590 533 399
259 240 233 193 185 158 138 134
99 85 59 35
BspEI T'CCGG_A
Cuts at: 8673 8673
Size: 9010
BspHI T'CATG_A
Cuts at: 6899 8629 6899
Size: 1730 7280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2382 2447 2632 2641 3052 3898 4360 4571
Size: 1538 65 185 9 411 846 462 211
Cuts at: 4571 4741 5365 6365 844
Size: 170 624 1000 3489
Fragments arranged by size:
3489 1538 1000 846 624 462 411 211
185 170 65 9
BsrBI CCG'CTC
Cuts at: 1394 1823 3635 6897 1394
Size: 429 1812 3262 3507
Fragments arranged by size:
3507 3262 1812 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2184 2552 3308 5367 6067 7505 7679
Size: 1183 936 368 756 2059 700 1438 174
Cuts at: 7679 65
Size: 1396
Fragments arranged by size:
2059 1438 1396 1183 936 756 700 368
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7654 7841 1700
Size: 110 5844 187 2869
Fragments arranged by size:
5844 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9010
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9010
BssSI C'ACGA_G
Cuts at: 7070 8082 7070
Size: 1012 7998
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2911 4025 4046 4906 794
Size: 2117 1114 21 860 4898
Fragments arranged by size:
4898 2117 1114 860 21
BstBI TT'CG_AA
Cuts at: 6197 7814 6197
Size: 1617 7393
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9010
BstXI CCAn_nnnn'nTGG
Cuts at: 5618 5618
Size: 9010
BstYI r'GATC_y
Cuts at: 1972 2759 3463 3864 5199 6340 6707 7092 7109
Size: 787 704 401 1335 1141 367 385 17
Cuts at: 7109 8561 8647 8659 9005 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1335 1141 787 704 401 385
367 346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3107 4893 6136 513
Size: 552 2042 1786 1243 3387
Fragments arranged by size:
3387 2042 1786 1243 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3558 3888 487
Size: 683 56 2332 330 5609
Fragments arranged by size:
5609 2332 683 330 56
BtsI GCAGTG_nn'
Cuts at: 2314 2362 5838 6526 7320 7340 2314
Size: 48 3476 688 794 20 3984
Fragments arranged by size:
3984 3476 794 688 48 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9010
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6223 6258 529
Size: 35 5659 35 3281
Fragments arranged by size:
5659 3281 35 35
DraI TTT'AAA
Cuts at: 4803 6314 4803
Size: 1511 7499
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2946 8017 809
Size: 507 1630 5071 1802
Fragments arranged by size:
5071 1802 1630 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5398 6139 6457
Size: 54 1425 50 275 18 3568 741 318
Cuts at: 6457 7346 8975 8
Size: 889 1629 43
Fragments arranged by size:
3568 1629 1425 889 741 318 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5398 6457 1537
Size: 275 3586 1059 4090
Fragments arranged by size:
4090 3586 1059 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2199 3333 3723 3746 6165 6940 1353
Size: 846 1134 390 23 2419 775 3423
Fragments arranged by size:
3423 2419 1134 846 775 390 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2060 7597 7971 8117 387
Size: 1064 138 471 5537 374 146 1280
Fragments arranged by size:
5537 1280 1064 471 374 146 138
EcoICRI GAG'CTC
Cuts at: 4573 4573
Size: 9010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2179 2677 2913 2988 3369 3859 4332
Size: 393 547 498 236 75 381 490 473
Cuts at: 4332 4593 5096 5128 5189 5463 5493 7073 7670
Size: 261 503 32 61 274 30 1580 597
Cuts at: 7670 8457 8725 1239
Size: 787 268 1524
Fragments arranged by size:
1580 1524 787 597 547 503 498 490
473 393 381 274 268 261 236 75
61 32 30
EcoNI CCTnn'n_nnAGG
Cuts at: 4086 4086
Size: 9010
EcoO109I rG'GnC_Cy
Cuts at: 2037 3733 4079 4180 4181 4472 4514 4583 5106
Size: 1696 346 101 1 291 42 69 523
Cuts at: 5106 5424 5642 2037
Size: 318 218 5405
Fragments arranged by size:
5405 1696 523 346 318 291 218 101
69 42 1
EcoRI G'AATT_C
Cuts at: 2993 3069 6319 6697 2993
Size: 76 3250 378 5306
Fragments arranged by size:
5306 3250 378 76
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2080 2123 2169 2080
Size: 43 46 8921
Fragments arranged by size:
8921 46 43
FspI TGC'GCA
Cuts at: 7516 7516
Size: 9010
HaeII r_GCGC'y
Cuts at: 1678 2513 4982 6249 8157 1678
Size: 835 2469 1267 1908 2531
Fragments arranged by size:
2531 2469 1908 1267 835
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2143 2175 2303 2335 2847
Size: 32 135 32 571 32 128 32 512
Cuts at: 2847 2879 3172 3204 5021 5053 5221 5253 5561
Size: 32 293 32 1817 32 168 32 308
Cuts at: 5561 5593 5979 6011 6014 6046 6062 6094 7651
Size: 32 386 32 3 32 16 32 1557
Cuts at: 7651 7683 7725 7757 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1817 1557 571 512 386 308 293
168 135 128 42 32 32 32 32
32 32 32 32 32 32 32 32
32 32 16 3
HincII GTy'rAC
Cuts at: 669 1657 1938 6354 6600 669
Size: 988 281 4416 246 3079
Fragments arranged by size:
4416 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4697 748
Size: 3949 5061
HpaI GTT'AAC
Cuts at: 6600 6600
Size: 9010
KpnI G_GTAC'C
Cuts at: 6335 6335
Size: 9010
MfeI C'AATT_G
Cuts at: 6609 6609
Size: 9010
MluI A'CGCG_T
Cuts at: 8886 8886
Size: 9010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2598 4042 4370 4532 8124 8308 1305
Size: 1293 1444 328 162 3592 184 2007
Fragments arranged by size:
3592 2007 1444 1293 328 184 162
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6141 10
Size: 54 1425 343 4309 2879
Fragments arranged by size:
4309 2879 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3242 6227 6968 7327 7486 518
Size: 648 2076 2985 741 359 159 2042
Fragments arranged by size:
2985 2076 2042 741 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3221 4025 4031 4043 4556 4870 5062 5293
Size: 1615 804 6 12 513 314 192 231
Cuts at: 5293 6426 7103 8251 8496 1606
Size: 1133 677 1148 245 2120
Fragments arranged by size:
2120 1615 1148 1133 804 677 513 314
245 231 192 12 6
NcoI C'CATG_G
Cuts at: 513 1065 3107 4893 6136 513
Size: 552 2042 1786 1243 3387
Fragments arranged by size:
3387 2042 1786 1243 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9010
NheI G'CTAG_C
Cuts at: 1052 5684 6110 1052
Size: 4632 426 3952
Fragments arranged by size:
4632 3952 426
NotI GC'GGCC_GC
Cuts at: 6457 6457
Size: 9010
NspI r_CATG'y
Cuts at: 1132 1229 2169 4371 6368 7913 1132
Size: 97 940 2202 1997 1545 2229
Fragments arranged by size:
2229 2202 1997 1545 940 97
PasI CC'CwG_GG
Cuts at: 1348 3918 4088 1348
Size: 2570 170 6270
Fragments arranged by size:
6270 2570 170
PciI A'CATG_T
Cuts at: 7909 7909
Size: 9010
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4082 4094 1108
Size: 2974 12 6024
Fragments arranged by size:
6024 2974 12
PfoI T'CCnGG_A
Cuts at: 2890 4075 4105 2890
Size: 1185 30 7795
Fragments arranged by size:
7795 1185 30
PmeI GTTT'AAAC
Cuts at: 6314 6314
Size: 9010
PmlI CAC'GTG
Cuts at: 3761 3761
Size: 9010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7079 7111 8592 8624 7079
Size: 32 1481 32 7465
Fragments arranged by size:
7465 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2037 4079 4514 4583 5424 5642 2037
Size: 2042 435 69 841 218 5405
Fragments arranged by size:
5405 2042 841 435 218 69
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9010
PsiI TTA'TAA
Cuts at: 3540 6580 3540
Size: 3040 5970
PspOMI G'GGCC_C
Cuts at: 4180 4472 4180
Size: 292 8718
PspXI vC'TCGA_Gb
Cuts at: 4753 4753
Size: 9010
PstI C_TGCA'G
Cuts at: 830 4051 5899 6362 830
Size: 3221 1848 463 3478
Fragments arranged by size:
3478 3221 1848 463
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9010
PvuII CAG'CTG
Cuts at: 3221 4025 4043 4556 4870 5062 5293 3221
Size: 804 18 513 314 192 231 6938
Fragments arranged by size:
6938 804 513 314 231 192 18
SacI G_AGCT'C
Cuts at: 4575 4575
Size: 9010
SalI G'TCGA_C
Cuts at: 1936 6352 1936
Size: 4416 4594
SbfI CC_TGCA'GG
Cuts at: 6362 6362
Size: 9010
ScaI AGT'ACT
Cuts at: 5560 7258 8970 5560
Size: 1698 1712 5600
Fragments arranged by size:
5600 1712 1698
SexAI A'CCwGG_T
Cuts at: 6040 6040
Size: 9010
SfcI C'TryA_G
Cuts at: 826 1045 4047 4322 4725 5895 5983 6358 7493
Size: 219 3002 275 403 1170 88 375 1135
Cuts at: 7493 8174 8365 826
Size: 681 191 1471
Fragments arranged by size:
3002 1471 1170 1135 681 403 375 275
219 191 88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9010
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9010
SmaI CCC'GGG
Cuts at: 1872 6337 1872
Size: 4465 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3701 3860 4753 7114 8015 8277
Size: 197 926 1758 159 893 2361 901 262
Cuts at: 8277 820
Size: 1553
Fragments arranged by size:
2361 1758 1553 926 901 893 262 197
159
SnaBI TAC'GTA
Cuts at: 493 6801 493
Size: 6308 2702
SpeI A'CTAG_T
Cuts at: 152 3944 152
Size: 3792 5218
SphI G_CATG'C
Cuts at: 1132 2169 4371 6368 1132
Size: 1037 2202 1997 3774
Fragments arranged by size:
3774 2202 1997 1037
SspI AAT'ATT
Cuts at: 5 52 5386 6934 5
Size: 47 5334 1548 2081
Fragments arranged by size:
5334 2081 1548 47
StuI AGG'CCT
Cuts at: 1517 3254 1517
Size: 1737 7273
StyI C'CwwG_G
Cuts at: 513 1065 3013 3107 3352 4893 5102 6136 6449
Size: 552 1948 94 245 1541 209 1034 313
Cuts at: 6449 513
Size: 3074
Fragments arranged by size:
3074 1948 1541 1034 552 313 245 209
94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2101 2458 3737 5924 5981 7037 7054
Size: 1255 521 357 1279 2187 57 1056 17
Cuts at: 7054 7207 7392 7837 325
Size: 153 185 445 1498
Fragments arranged by size:
2187 1498 1279 1255 1056 521 445 357
185 153 57 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5558 7256 8968
Size: 274 80 33 51 1443 3581 1698 1712
Cuts at: 8968 96
Size: 138
Fragments arranged by size:
3581 1712 1698 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2177 2209 8970 9002 2177
Size: 32 6761 32 2185
Fragments arranged by size:
6761 2185 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4101 4160 1316
Size: 2785 59 6166
Fragments arranged by size:
6166 2785 59
XbaI T'CTAG_A
Cuts at: 6346 6346
Size: 9010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4192 5359 4192
Size: 1167 7843
XhoI C'TCGA_G
Cuts at: 1943 4753 1943
Size: 2810 6200
XmaI C'CCGG_G
Cuts at: 1870 6335 1870
Size: 4465 4545
XmnI GAAnn'nnTTC
Cuts at: 2106 2750 4698 7139 2106
Size: 644 1948 2441 3977
Fragments arranged by size:
3977 2441 1948 644
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6818 276
Size: 53 83 186 936 5284 2468
Fragments arranged by size:
5284 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PsrI RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI