Restriction map for
pFN21AA1677
(Circular) (Six-base) MAPSORT of: pFN21AA1677.seq Check: 194 from: 1 to: 6517
pFN21AA1677 6517 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4327 278
Size: 53 83 186 936 2791 2468
Fragments arranged by size:
2791 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3546 3860 1937
Size: 1609 314 4594
Fragments arranged by size:
4594 1609 314
Acc65I G'GTAC_C
Cuts at: 3838 3838
Size: 6517
AclI AA'CG_TT
Cuts at: 1563 3816 4644 5017 1563
Size: 2253 828 373 3063
Fragments arranged by size:
3063 2253 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2155 2303 4580 5964 6232 1632
Size: 523 148 2277 1384 268 1917
Fragments arranged by size:
2277 1917 1384 523 268 148
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6320
AflIII A'CryG_T
Cuts at: 1203 5416 6393 1203
Size: 4213 977 1327
Fragments arranged by size:
4213 1327 977
AgeI A'CCGG_T
Cuts at: 2243 3296 5348 2243
Size: 1053 2052 3412
Fragments arranged by size:
3412 2052 1053
AhdI GACnn_n'nnGTC
Cuts at: 2518 5246 2518
Size: 2728 3789
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6517
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6517
AlwNI CAG_nnn'CTG
Cuts at: 3560 4260 5832 3560
Size: 700 1572 4245
Fragments arranged by size:
4245 1572 700
ApaI G_GGCC'C
Cuts at: 3020 3020
Size: 6517
ApaLI G'TGCA_C
Cuts at: 4574 5730 4574
Size: 1156 5361
ApoI r'AATT_y
Cuts at: 1493 2217 2723 3029 3116 3224 3826 4052 4204
Size: 724 506 306 87 108 602 226 152
Cuts at: 4204 6313 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 724 602 506 306 226 152
108 87
AseI AT'TA_AT
Cuts at: 160 1032 5071 160
Size: 872 4039 1606
Fragments arranged by size:
4039 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6517
AvaI C'yCGr_G
Cuts at: 1870 1943 3064 3842 1870
Size: 73 1121 778 4545
Fragments arranged by size:
4545 1121 778 73
BamHI G'GATC_C
Cuts at: 3232 3847 4214 3232
Size: 615 367 5535
Fragments arranged by size:
5535 615 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3838 5293 618
Size: 325 217 627 2051 1455 1842
Fragments arranged by size:
2051 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3020 3836 1391
Size: 1629 816 4072
Fragments arranged by size:
4072 1629 816
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2638 3472 928
Size: 971 739 834 3973
Fragments arranged by size:
3973 971 834 739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2946 2980 4708 4742 1903
Size: 34 1009 34 1728 34 3678
Fragments arranged by size:
3678 1728 1009 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4409 5619 4409
Size: 1210 5307
BclI T'GATC_A
Cuts at: 1548 2563 3562 1548
Size: 1015 999 4503
Fragments arranged by size:
4503 1015 999
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5128 136
Size: 107 122 71 631 605 3456 1525
Fragments arranged by size:
3456 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2385 6512 2385
Size: 4127 2390
BlpI GC'TnA_GC
Cuts at: 3934 3934
Size: 6517
Bme1580I G_kGCm'C
Cuts at: 1790 3020 4578 5734 1790
Size: 1230 1558 1156 2573
Fragments arranged by size:
2573 1558 1230 1156
BmgBI CAC'GTC
Cuts at: 1318 2017 1318
Size: 699 5818
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4248 5206 442
Size: 416 53 83 399 24 2831 958 1753
Fragments arranged by size:
2831 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2659 5177 1239
Size: 1420 2518 2579
Fragments arranged by size:
2579 2518 1420
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3372 3523 4642 5507 5805 3372
Size: 151 1119 865 298 4084
Fragments arranged by size:
4084 1119 865 298 151
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5180 882
Size: 617 3681 2219
Fragments arranged by size:
3681 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4308 493
Size: 711 3104 2702
Fragments arranged by size:
3104 2702 711
BsaBI GATnn'nnATC
Cuts at: 2562 3237 6511 2562
Size: 675 3274 2568
Fragments arranged by size:
3274 2568 675
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4324 4706 5341 275
Size: 53 83 186 936 2791 382 635 1451
Fragments arranged by size:
2791 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2243 2670 3296 3697 3781 4950 5348 5622 5769
Size: 427 626 401 84 1169 398 274 147
Cuts at: 5769 6180 2243
Size: 411 2580
Fragments arranged by size:
2580 1169 626 427 411 401 398 274
147 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6292
Fragments arranged by size:
6292 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 1186
Size: 910 5607
BseYI C'CCAG_C
Cuts at: 2060 3013 3347 5720 6216 2060
Size: 953 334 2373 496 2361
Fragments arranged by size:
2373 2361 953 496 334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2424 2627 1163
Size: 573 688 203 5053
Fragments arranged by size:
5053 688 573 203
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3967 4728 5756 1540
Size: 275 186 1966 761 1028 2301
Fragments arranged by size:
2301 1966 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2074 3276 4578 4663 5734 2074
Size: 1202 1302 85 1071 2857
Fragments arranged by size:
2857 1302 1202 1071 85
BsmI GAATG_Cn'
Cuts at: 4028 4121 4028
Size: 93 6424
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 3020 3276 3836 4578 4663 5734
Size: 399 284 946 256 560 742 85 1071
Cuts at: 5734 1391
Size: 2174
Fragments arranged by size:
2174 1071 946 742 560 399 284 256
85
BspEI T'CCGG_A
Cuts at: 3697 3781 6180 3697
Size: 84 2399 4034
Fragments arranged by size:
4034 2399 84
BspHI T'CATG_A
Cuts at: 2308 4406 6136 2308
Size: 2098 1730 2689
Fragments arranged by size:
2689 2098 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2565 3780 3872 844
Size: 1721 1215 92 3489
Fragments arranged by size:
3489 1721 1215 92
BsrBI CCG'CTC
Cuts at: 1394 1823 4404 1394
Size: 429 2581 3507
Fragments arranged by size:
3507 2581 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5012 5186 65
Size: 1183 3764 174 1396
Fragments arranged by size:
3764 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2243 3296 5161 5348 1700
Size: 110 433 1053 1865 187 2869
Fragments arranged by size:
2869 1865 1053 433 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6517
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6517
BssSI C'ACGA_G
Cuts at: 4577 5589 4577
Size: 1012 5505
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6517
BstBI TT'CG_AA
Cuts at: 3516 5321 3516
Size: 1805 4712
BstEII G'GTnAC_C
Cuts at: 1184 3394 1184
Size: 2210 4307
BstXI CCAn_nnnn'nTGG
Cuts at: 2922 2922
Size: 6517
BstYI r'GATC_y
Cuts at: 1972 2385 3232 3847 4214 4599 4616 6068 6154
Size: 413 847 615 367 385 17 1452 86
Cuts at: 6154 6166 6512 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 847 615 413 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3547 3547
Size: 6517
BtgI C'CryG_G
Cuts at: 513 1065 2004 3607 513
Size: 552 939 1603 3423
Fragments arranged by size:
3423 1603 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5778
Fragments arranged by size:
5778 683 56
BtsI GCAGTG_nn'
Cuts at: 4033 4827 4847 4033
Size: 794 20 5703
Fragments arranged by size:
5703 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6517
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6482
DraI TTT'AAA
Cuts at: 2226 3821 2226
Size: 1595 4922
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2051 5524 809
Size: 507 735 3473 1802
Fragments arranged by size:
3473 1802 735 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2151 3604 3964
Size: 54 1425 50 275 18 321 1453 360
Cuts at: 3964 4853 6482 8
Size: 889 1629 43
Fragments arranged by size:
1629 1453 1425 889 360 321 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3964 1537
Size: 275 2152 4090
Fragments arranged by size:
4090 2152 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3003 4447 1353
Size: 1650 1444 3423
Fragments arranged by size:
3423 1650 1444
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5104 5478 5624 387
Size: 1064 138 3515 374 146 1280
Fragments arranged by size:
3515 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2155 2303 2659 4580 5177 5964 6232
Size: 393 523 148 356 1921 597 787 268
Cuts at: 6232 1239
Size: 1524
Fragments arranged by size:
1921 1524 787 597 523 393 356 268
148
EcoO109I rG'GnC_Cy
Cuts at: 3507 3507
Size: 6517
EcoRI G'AATT_C
Cuts at: 3029 3826 4204 3029
Size: 797 378 5342
Fragments arranged by size:
5342 797 378
FspI TGC'GCA
Cuts at: 5023 5023
Size: 6517
HaeII r_GCGC'y
Cuts at: 1678 5664 1678
Size: 3986 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2504 2536 2801 2833 3189
Size: 32 135 32 932 32 265 32 356
Cuts at: 3189 3221 5158 5190 5232 5264 1373
Size: 32 1937 32 42 32 2626
Fragments arranged by size:
2626 1937 932 356 265 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2761 2887 3247 3861 4107 669
Size: 988 281 823 126 360 614 246 3079
Fragments arranged by size:
3079 988 823 614 360 281 246 126
HindIII A'AGCT_T
Cuts at: 748 3470 748
Size: 2722 3795
HpaI GTT'AAC
Cuts at: 2887 3247 4107 2887
Size: 360 860 5297
Fragments arranged by size:
5297 860 360
KpnI G_GTAC'C
Cuts at: 3842 3842
Size: 6517
MfeI C'AATT_G
Cuts at: 4116 4116
Size: 6517
MluI A'CGCG_T
Cuts at: 6393 6393
Size: 6517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2787 5631 5815 1305
Size: 1482 2844 184 2007
Fragments arranged by size:
2844 2007 1482 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2153 3606 10
Size: 54 1425 343 321 1453 2921
Fragments arranged by size:
2921 1453 1425 343 321 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4475 4834 4993 518
Size: 648 3309 359 159 2042
Fragments arranged by size:
3309 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3933 4610 5758 6003 1606
Size: 2327 677 1148 245 2120
Fragments arranged by size:
2327 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3607 513
Size: 552 939 1603 3423
Fragments arranged by size:
3423 1603 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6517
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6517
NotI GC'GGCC_GC
Cuts at: 3964 3964
Size: 6517
NspI r_CATG'y
Cuts at: 1132 1229 3875 5420 1132
Size: 97 2646 1545 2229
Fragments arranged by size:
2646 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6517
PciI A'CATG_T
Cuts at: 5416 5416
Size: 6517
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3405 1108
Size: 2297 4220
PmeI GTTT'AAAC
Cuts at: 3821 3821
Size: 6517
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4586 4618 6099 6131 4586
Size: 32 1481 32 4972
Fragments arranged by size:
4972 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6517
PsiI TTA'TAA
Cuts at: 3660 4087 3660
Size: 427 6090
PspOMI G'GGCC_C
Cuts at: 3016 3016
Size: 6517
PstI C_TGCA'G
Cuts at: 830 2191 3087 3869 830
Size: 1361 896 782 3478
Fragments arranged by size:
3478 1361 896 782
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6517
SalI G'TCGA_C
Cuts at: 1936 3859 1936
Size: 1923 4594
SapI GCTCTTCn'nnn_
Cuts at: 3003 3003
Size: 6517
SbfI CC_TGCA'GG
Cuts at: 3869 3869
Size: 6517
ScaI AGT'ACT
Cuts at: 4765 6477 4765
Size: 1712 4805
SfcI C'TryA_G
Cuts at: 826 1045 2187 2283 2850 3083 3123 3537 3865
Size: 219 1142 96 567 233 40 414 328
Cuts at: 3865 5000 5681 5872 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1142 1135 681 567 414 328 233
219 191 96 40
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6517
SgrAI Cr'CCGG_yG
Cuts at: 1700 3296 1700
Size: 1596 4921
SmaI CCC'GGG
Cuts at: 1872 3844 1872
Size: 1972 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3387 3502 4621 5522 5784 820
Size: 197 926 1444 115 1119 901 262 1553
Fragments arranged by size:
1553 1444 1119 926 901 262 197 115
SnaBI TAC'GTA
Cuts at: 493 4308 493
Size: 3815 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6517
SphI G_CATG'C
Cuts at: 1132 3875 1132
Size: 2743 3774
SspI AAT'ATT
Cuts at: 5 52 4441 5
Size: 47 4389 2081
Fragments arranged by size:
4389 2081 47
StuI AGG'CCT
Cuts at: 1517 2509 3651 1517
Size: 992 1142 4383
Fragments arranged by size:
4383 1142 992
StyI C'CwwG_G
Cuts at: 513 1065 2004 3607 3956 513
Size: 552 939 1603 349 3074
Fragments arranged by size:
3074 1603 939 552 349
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2171 4544 4561 4714 4899 5344 325
Size: 1255 591 2373 17 153 185 445 1498
Fragments arranged by size:
2373 1498 1255 591 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2271 3790 4763
Size: 274 80 33 51 1443 294 1519 973
Cuts at: 4763 6475 96
Size: 1712 138
Fragments arranged by size:
1712 1519 1443 973 294 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3679 3711 6477 6509 3679
Size: 32 2766 32 3687
Fragments arranged by size:
3687 2766 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6517
XbaI T'CTAG_A
Cuts at: 3853 3853
Size: 6517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2922 2922
Size: 6517
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6517
XmaI C'CCGG_G
Cuts at: 1870 3842 1870
Size: 1972 4545
XmnI GAAnn'nnTTC
Cuts at: 4646 4646
Size: 6517
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4325 276
Size: 53 83 186 936 2791 2468
Fragments arranged by size:
2791 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI