Restriction map for
pFN21AA1675
(Circular) (Six-base) MAPSORT of: pFN21AA1675.seq Check: 5444 from: 1 to: 8533
pFN21AA1675 8533 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3825 6343 278
Size: 53 83 186 936 2289 2518 2468
Fragments arranged by size:
2518 2468 2289 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2220 2646 5876 1937
Size: 283 426 3230 4594
Fragments arranged by size:
4594 3230 426 283
Acc65I G'GTAC_C
Cuts at: 5854 5854
Size: 8533
AclI AA'CG_TT
Cuts at: 1563 6660 7033 1563
Size: 5097 373 3063
Fragments arranged by size:
5097 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2250 3044 3797 4670 4951 6596 7980 8248
Size: 618 794 753 873 281 1645 1384 268
Cuts at: 8248 1632
Size: 1917
Fragments arranged by size:
1917 1645 1384 873 794 753 618 281
268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8336
AflIII A'CryG_T
Cuts at: 1203 2757 2759 3795 4857 7432 8409 1203
Size: 1554 2 1036 1062 2575 977 1327
Fragments arranged by size:
2575 1554 1327 1062 1036 977 2
AgeI A'CCGG_T
Cuts at: 7364 7364
Size: 8533
AhdI GACnn_n'nnGTC
Cuts at: 5431 7262 5431
Size: 1831 6702
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4784 1184
Size: 3600 4933
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2675 2707 2675
Size: 32 8501
AlwNI CAG_nnn'CTG
Cuts at: 2285 4051 4192 4411 4869 6276 7848 2285
Size: 1766 141 219 458 1407 1572 2970
Fragments arranged by size:
2970 1766 1572 1407 458 219 141
ApaI G_GGCC'C
Cuts at: 2339 3100 3729 3979 4379 4438 2339
Size: 761 629 250 400 59 6434
Fragments arranged by size:
6434 761 629 400 250 59
ApaLI G'TGCA_C
Cuts at: 2627 6590 7746 2627
Size: 3963 1156 3414
Fragments arranged by size:
3963 3414 1156
ApoI r'AATT_y
Cuts at: 1493 5842 6068 6220 8329 1493
Size: 4349 226 152 2109 1697
Fragments arranged by size:
4349 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7087 160
Size: 872 6055 1606
Fragments arranged by size:
6055 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8533
AvaI C'yCGr_G
Cuts at: 1870 1943 2355 2813 3093 3379 3487 3547 3728
Size: 73 412 458 280 286 108 60 181
Cuts at: 3728 3970 4278 4756 5126 5444 5858 1870
Size: 242 308 478 370 318 414 4545
Fragments arranged by size:
4545 478 458 414 412 370 318 308
286 280 242 181 108 73 60
BamHI G'GATC_C
Cuts at: 5863 6230 5863
Size: 367 8166
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2350 2742 3511 3964 3997
Size: 325 217 627 563 392 769 453 33
Cuts at: 3997 4064 4505 5079 5854 7309 618
Size: 67 441 574 775 1455 1842
Fragments arranged by size:
1842 1455 775 769 627 574 563 453
441 392 325 217 67 33
BanII G_rGCy'C
Cuts at: 1391 2076 2201 2262 2339 2688 2808 3100 3617
Size: 685 125 61 77 349 120 292 517
Cuts at: 3617 3729 3872 3979 4157 4237 4379 4438 4470
Size: 112 143 107 178 80 142 59 32
Cuts at: 4470 4648 5451 5852 1391
Size: 178 803 401 4072
Fragments arranged by size:
4072 803 685 517 401 349 292 178
178 143 142 125 120 112 107 80
77 61 59 32
BbeI G_GCGC'C
Cuts at: 2746 3515 3968 4068 4509 2746
Size: 769 453 100 441 6770
Fragments arranged by size:
6770 769 453 441 100
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2238 2457 3128 3785 3997 5217 5736
Size: 971 339 219 671 657 212 1220 519
Cuts at: 5736 928
Size: 3725
Fragments arranged by size:
3725 1220 971 671 657 519 339 219
212
BbvCI CC'TCA_GC
Cuts at: 2799 5532 5610 2799
Size: 2733 78 5722
Fragments arranged by size:
5722 2733 78
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2108 2142 2995 3029 4842
Size: 34 50 34 87 34 853 34 1813
Cuts at: 4842 4876 6724 6758 1903
Size: 34 1848 34 3678
Fragments arranged by size:
3678 1848 1813 853 87 50 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6425 7635 6425
Size: 1210 7323
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8533
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4452 4748 5498
Size: 107 122 71 631 605 2780 296 750
Cuts at: 5498 7144 136
Size: 1646 1525
Fragments arranged by size:
2780 1646 1525 750 631 605 296 122
107 71
BglII A'GATC_T
Cuts at: 2442 4878 8528 2442
Size: 2436 3650 2447
Fragments arranged by size:
3650 2447 2436
BlpI GC'TnA_GC
Cuts at: 5950 5950
Size: 8533
Bme1580I G_kGCm'C
Cuts at: 1790 2339 2355 2631 2660 3100 3710 3729 3979
Size: 549 16 276 29 440 610 19 250
Cuts at: 3979 4319 4379 4438 4535 5320 6594 7750 1790
Size: 340 60 59 97 785 1274 1156 2573
Fragments arranged by size:
2573 1274 1156 785 610 549 440 340
276 250 97 60 59 29 19 16
BmgBI CAC'GTC
Cuts at: 1318 2407 3135 3889 4348 4483 4996 5356 5796
Size: 1089 728 754 459 135 513 360 440
Cuts at: 5796 1318
Size: 4055
Fragments arranged by size:
4055 1089 754 728 513 459 440 360
135
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3642 4299 4935
Size: 416 53 83 399 24 2225 657 636
Cuts at: 4935 5314 6264 7222 442
Size: 379 950 958 1753
Fragments arranged by size:
2225 1753 958 950 657 636 416 399
379 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2036 1056
Size: 980 7553
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2912 3460 3773 4748 4970 5402 5808 5826
Size: 1673 548 313 975 222 432 406 18
Cuts at: 5826 7193 1239
Size: 1367 2579
Fragments arranged by size:
2579 1673 1367 975 548 432 406 313
222 18
Bpu10I CC'TnA_GC
Cuts at: 2799 4418 5532 5610 2799
Size: 1619 1114 78 5722
Fragments arranged by size:
5722 1619 1114 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2495 2792 3131 3593 4274 5069 6658 7523 7821
Size: 297 339 462 681 795 1589 865 298
Cuts at: 7821 2495
Size: 3207
Fragments arranged by size:
3207 1589 865 795 681 462 339 298
297
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3685 4703 7196 882
Size: 617 2186 1018 2493 2219
Fragments arranged by size:
2493 2219 2186 1018 617
BsaAI yAC'GTr
Cuts at: 493 1204 2632 2760 3085 4860 6324 493
Size: 711 1428 128 325 1775 1464 2702
Fragments arranged by size:
2702 1775 1464 1428 711 325 128
BsaBI GATnn'nnATC
Cuts at: 2484 8527 2484
Size: 6043 2490
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2643 2743 3512 3636
Size: 53 83 186 936 1110 100 769 124
Cuts at: 3636 3822 3965 4065 4284 4506 6340 6722 7357
Size: 186 143 100 219 222 1834 382 635
Cuts at: 7357 275
Size: 1451
Fragments arranged by size:
1834 1451 1110 936 769 635 382 222
219 186 186 143 124 100 100 83
53
BsaWI w'CCGG_w
Cuts at: 4800 4998 6966 7364 7638 7785 8196 4800
Size: 198 1968 398 274 147 411 5137
Fragments arranged by size:
5137 1968 411 398 274 198 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2675 2705 2912 2942 3458
Size: 30 165 30 1272 30 207 30 516
Cuts at: 3458 3488 3499 3529 4922 4952 5735 5765 1178
Size: 30 11 30 1393 30 783 30 3946
Fragments arranged by size:
3946 1393 1272 783 516 207 165 30
30 30 30 30 30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2630 2696 2816 2933 3301 3746 4415 5375
Size: 1444 66 120 117 368 445 669 960
Cuts at: 5375 5378 5399 5486 5495 5727 1186
Size: 3 21 87 9 232 3992
Fragments arranged by size:
3992 1444 960 669 445 368 232 120
117 87 66 21 9 3
BseYI C'CCAG_C
Cuts at: 2245 2332 3050 3398 3431 3664 4014 4150 4501
Size: 87 718 348 33 233 350 136 351
Cuts at: 4501 4548 7736 8232 2245
Size: 47 3188 496 2546
Fragments arranged by size:
3188 2546 718 496 351 350 348 233
136 87 47 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4882 5701 1163
Size: 573 3146 819 3995
Fragments arranged by size:
3995 3146 819 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3581 4670 4960 5983 6744 7772
Size: 275 186 1580 1089 290 1023 761 1028
Cuts at: 7772 1540
Size: 2301
Fragments arranged by size:
2301 1580 1089 1028 1023 761 290 275
186
BsiHKAI G_wGCw'C
Cuts at: 2262 2534 2631 2808 5100 6594 6679 7750 2262
Size: 272 97 177 2292 1494 85 1071 3045
Fragments arranged by size:
3045 2292 1494 1071 272 177 97 85
BsiWI C'GTAC_G
Cuts at: 2205 2205
Size: 8533
BsmI GAATG_Cn'
Cuts at: 6044 6137 6044
Size: 93 8440
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2201 2262 2339 2355 2534 2631
Size: 399 286 125 61 77 16 179 97
Cuts at: 2631 2660 2688 2808 3100 3617 3710 3729 3872
Size: 29 28 120 292 517 93 19 143
Cuts at: 3872 3979 4157 4237 4319 4379 4438 4470 4535
Size: 107 178 80 82 60 59 32 65
Cuts at: 4535 4648 5100 5320 5451 5852 6594 6679 7750
Size: 113 452 220 131 401 742 85 1071
Cuts at: 7750 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 517 452 401 399 292
286 220 179 178 143 131 125 120
113 107 97 93 85 82 80 77
65 61 60 59 32 29 28 19
16
BspEI T'CCGG_A
Cuts at: 4998 8196 4998
Size: 3198 5335
BspHI T'CATG_A
Cuts at: 6422 8152 6422
Size: 1730 6803
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2094 2745 4671 4854 5888 844
Size: 1250 651 1926 183 1034 3489
Fragments arranged by size:
3489 1926 1250 1034 651 183
BsrBI CCG'CTC
Cuts at: 1394 1823 2866 3075 4561 4962 6420 1394
Size: 429 1043 209 1486 401 1458 3507
Fragments arranged by size:
3507 1486 1458 1043 429 401 209
BsrDI GCAATG_nn'
Cuts at: 65 1248 4098 5715 7028 7202 65
Size: 1183 2850 1617 1313 174 1396
Fragments arranged by size:
2850 1617 1396 1313 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3234 3327 4508 5076 7177 7364 1700
Size: 110 1424 93 1181 568 2101 187 2869
Fragments arranged by size:
2869 2101 1424 1181 568 187 110 93
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8533
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8533
BssSI C'ACGA_G
Cuts at: 2846 5093 5099 6593 7605 2846
Size: 2247 6 1494 1012 3774
Fragments arranged by size:
3774 2247 1494 1012 6
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2859 4100 5049 794
Size: 2065 1241 949 4278
Fragments arranged by size:
4278 2065 1241 949
BstBI TT'CG_AA
Cuts at: 3015 4979 7337 3015
Size: 1964 2358 4211
Fragments arranged by size:
4211 2358 1964
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8533
BstXI CCAn_nnnn'nTGG
Cuts at: 2146 3655 4487 2146
Size: 1509 832 6192
Fragments arranged by size:
6192 1509 832
BstYI r'GATC_y
Cuts at: 1972 2442 4878 5863 6230 6615 6632 8084 8170
Size: 470 2436 985 367 385 17 1452 86
Cuts at: 8170 8182 8528 1972
Size: 12 346 1977
Fragments arranged by size:
2436 1977 1452 985 470 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4835 4913 4835
Size: 78 8455
BtgI C'CryG_G
Cuts at: 513 1065 2145 3507 3834 3858 4638 5502 513
Size: 552 1080 1362 327 24 780 864 3544
Fragments arranged by size:
3544 1362 1080 864 780 552 327 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2553 4075 487
Size: 683 56 1327 1522 4945
Fragments arranged by size:
4945 1522 1327 683 56
BtsI GCAGTG_nn'
Cuts at: 2467 3814 5320 6049 6843 6863 2467
Size: 1347 1506 729 794 20 4137
Fragments arranged by size:
4137 1506 1347 794 729 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3268 3303 529
Size: 35 2704 35 5759
Fragments arranged by size:
5759 2704 35 35
DraI TTT'AAA
Cuts at: 5667 5837 5667
Size: 170 8363
DraIII CAC_nnn'GTG
Cuts at: 2559 2559
Size: 8533
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5158 7540 809
Size: 507 3842 2382 1802
Fragments arranged by size:
3842 2382 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2836 3289
Size: 54 1425 50 275 18 261 745 453
Cuts at: 3289 3578 3990 4667 5406 5499 5980 6869 8498
Size: 289 412 677 739 93 481 889 1629
Cuts at: 8498 8
Size: 43
Fragments arranged by size:
1629 1425 889 745 739 677 481 453
412 289 275 261 93 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3578 4667 5980 1537
Size: 275 1766 1089 1313 4090
Fragments arranged by size:
4090 1766 1313 1089 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2723 4688 4981 6463 1353
Size: 1370 1965 293 1482 3423
Fragments arranged by size:
3423 1965 1482 1370 293
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2969 3673 3919 4766 5306 7120
Size: 1064 138 1380 704 246 847 540 1814
Cuts at: 7120 7494 7640 387
Size: 374 146 1280
Fragments arranged by size:
1814 1380 1280 1064 847 704 540 374
246 146 138
EcoICRI GAG'CTC
Cuts at: 2260 2806 2260
Size: 546 7987
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2250 2912 3044 3460 3773 3797 4670
Size: 393 618 662 132 416 313 24 873
Cuts at: 4670 4748 4951 4970 5402 5808 5826 6596 7193
Size: 78 203 19 432 406 18 770 597
Cuts at: 7193 7980 8248 1239
Size: 787 268 1524
Fragments arranged by size:
1524 873 787 770 662 618 597 432
416 406 393 313 268 203 132 78
24 19 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2678 2798 4838 5384 2678
Size: 120 2040 546 5827
Fragments arranged by size:
5827 2040 546 120
EcoO109I rG'GnC_Cy
Cuts at: 2083 2212 2336 2791 2818 3725 3975 3976 4375
Size: 129 124 455 27 907 250 1 399
Cuts at: 4375 4376 5131 2083
Size: 1 755 5485
Fragments arranged by size:
5485 907 755 455 399 250 129 124
27 1 1
EcoRI G'AATT_C
Cuts at: 5842 6220 5842
Size: 378 8155
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5654 5654
Size: 8533
FspI TGC'GCA
Cuts at: 7039 7039
Size: 8533
HaeII r_GCGC'y
Cuts at: 1678 2746 3093 3334 3515 3968 4068 4509 7680
Size: 1068 347 241 181 453 100 441 3171
Cuts at: 7680 1678
Size: 2531
Fragments arranged by size:
3171 2531 1068 453 441 347 241 181
100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4256 4288 5417 5449 7174
Size: 32 135 32 2684 32 1129 32 1725
Cuts at: 7174 7206 7248 7280 1373
Size: 32 42 32 2626
Fragments arranged by size:
2684 2626 1725 1129 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3826 5877 6123 669
Size: 988 281 1888 2051 246 3079
Fragments arranged by size:
3079 2051 1888 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8533
HpaI GTT'AAC
Cuts at: 6123 6123
Size: 8533
KasI G'GCGC_C
Cuts at: 2742 3511 3964 4064 4505 2742
Size: 769 453 100 441 6770
Fragments arranged by size:
6770 769 453 441 100
KpnI G_GTAC'C
Cuts at: 5858 5858
Size: 8533
MfeI C'AATT_G
Cuts at: 6132 6132
Size: 8533
MluI A'CGCG_T
Cuts at: 8409 8409
Size: 8533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2994 3249 3780 4848 5160 5778 7647 7831
Size: 1689 255 531 1068 312 618 1869 184
Cuts at: 7831 1305
Size: 2007
Fragments arranged by size:
2007 1869 1689 1068 618 531 312 255
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2093 3291 10
Size: 54 1425 343 261 1198 5252
Fragments arranged by size:
5252 1425 1198 343 261 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 2522 4133 4485 6491 6850 7009
Size: 648 978 378 1611 352 2006 359 159
Cuts at: 7009 518
Size: 2042
Fragments arranged by size:
2042 2006 1611 978 648 378 359 352
159
MspA1I CmG'CkG
Cuts at: 1606 2505 2697 2835 3003 3064 3604 3719 4048
Size: 899 192 138 168 61 540 115 329
Cuts at: 4048 4411 4678 4990 5049 5609 5949 6626 7774
Size: 363 267 312 59 560 340 677 1148
Cuts at: 7774 8019 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 899 677 560 540 363 340
329 312 267 245 192 168 138 115
61 59
NaeI GCC'GGC
Cuts at: 3236 4510 3236
Size: 1274 7259
NarI GG'CG_CC
Cuts at: 2743 3512 3965 4065 4506 2743
Size: 769 453 100 441 6770
Fragments arranged by size:
6770 769 453 441 100
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7981
NdeI CA'TA_TG
Cuts at: 387 3188 5704 387
Size: 2801 2516 3216
Fragments arranged by size:
3216 2801 2516
NgoMIV G'CCGG_C
Cuts at: 3234 4508 3234
Size: 1274 7259
NheI G'CTAG_C
Cuts at: 1052 2032 1052
Size: 980 7553
NotI GC'GGCC_GC
Cuts at: 5980 5980
Size: 8533
NspI r_CATG'y
Cuts at: 1132 1229 3799 5299 5891 7436 1132
Size: 97 2570 1500 592 1545 2229
Fragments arranged by size:
2570 2229 1545 1500 592 97
PasI CC'CwG_GG
Cuts at: 1348 2279 2653 3655 5755 1348
Size: 931 374 1002 2100 4126
Fragments arranged by size:
4126 2100 1002 931 374
PciI A'CATG_T
Cuts at: 3795 7432 3795
Size: 3637 4896
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2285 1108
Size: 1177 7356
PmeI GTTT'AAAC
Cuts at: 5837 5837
Size: 8533
PmlI CAC'GTG
Cuts at: 2632 2760 3085 4860 2632
Size: 128 325 1775 6305
Fragments arranged by size:
6305 1775 325 128
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2675 2707 2855 2887 3722 3754 6602 6634 8115
Size: 32 148 32 835 32 2848 32 1481
Cuts at: 8115 8147 2675
Size: 32 3061
Fragments arranged by size:
3061 2848 1481 835 148 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2083 5131 2083
Size: 3048 5485
PshAI GACnn'nnGTC
Cuts at: 1613 2943 1613
Size: 1330 7203
PsiI TTA'TAA
Cuts at: 6103 6103
Size: 8533
PspOMI G'GGCC_C
Cuts at: 2335 3096 3725 3975 4375 4434 2335
Size: 761 629 250 400 59 6434
Fragments arranged by size:
6434 761 629 400 250 59
PstI C_TGCA'G
Cuts at: 830 4773 5885 830
Size: 3943 1112 3478
Fragments arranged by size:
3943 3478 1112
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8533
PvuII CAG'CTG
Cuts at: 2505 3719 4048 5609 2505
Size: 1214 329 1561 5429
Fragments arranged by size:
5429 1561 1214 329
RsrII CG'GwC_CG
Cuts at: 4228 4228
Size: 8533
SacI G_AGCT'C
Cuts at: 2262 2808 2262
Size: 546 7987
SalI G'TCGA_C
Cuts at: 1936 5875 1936
Size: 3939 4594
SapI GCTCTTCn'nnn_
Cuts at: 4688 4688
Size: 8533
SbfI CC_TGCA'GG
Cuts at: 5885 5885
Size: 8533
ScaI AGT'ACT
Cuts at: 3467 4112 4952 6781 8493 3467
Size: 645 840 1829 1712 3507
Fragments arranged by size:
3507 1829 1712 840 645
SfcI C'TryA_G
Cuts at: 826 1045 4603 4769 5881 7016 7697 7888 826
Size: 219 3558 166 1112 1135 681 191 1471
Fragments arranged by size:
3558 1471 1135 1112 681 219 191 166
SfoI GGC'GCC
Cuts at: 2744 3513 3966 4066 4507 2744
Size: 769 453 100 441 6770
Fragments arranged by size:
6770 769 453 441 100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8533
SgrAI Cr'CCGG_yG
Cuts at: 1700 3327 4508 1700
Size: 1627 1181 5725
Fragments arranged by size:
5725 1627 1181
SmaI CCC'GGG
Cuts at: 1872 2357 3381 3489 3549 3972 4280 5128 5860
Size: 485 1024 108 60 423 308 848 732
Cuts at: 5860 1872
Size: 4545
Fragments arranged by size:
4545 1024 848 732 485 423 308 108
60
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2510 2807 3146 3608 4289 5084
Size: 197 926 567 297 339 462 681 795
Cuts at: 5084 6637 7538 7800 820
Size: 1553 901 262 1553
Fragments arranged by size:
1553 1553 926 901 795 681 567 462
339 297 262 197
SnaBI TAC'GTA
Cuts at: 493 6324 493
Size: 5831 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8533
SphI G_CATG'C
Cuts at: 1132 5891 1132
Size: 4759 3774
SspI AAT'ATT
Cuts at: 5 52 6457 5
Size: 47 6405 2081
Fragments arranged by size:
6405 2081 47
StuI AGG'CCT
Cuts at: 1517 2954 5331 1517
Size: 1437 2377 4719
Fragments arranged by size:
4719 2377 1437
StyI C'CwwG_G
Cuts at: 513 1065 2013 2190 2821 2949 4371 5972 513
Size: 552 948 177 631 128 1422 1601 3074
Fragments arranged by size:
3074 1601 1422 948 631 552 177 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3644 3857 4245 6560 6577 6730 6915
Size: 1255 2064 213 388 2315 17 153 185
Cuts at: 6915 7360 325
Size: 445 1498
Fragments arranged by size:
2315 2064 1498 1255 445 388 213 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2514 2754 2883
Size: 274 80 33 51 1443 537 240 129
Cuts at: 2883 2928 3465 4110 4950 6779 8491 96
Size: 45 537 645 840 1829 1712 138
Fragments arranged by size:
1829 1712 1443 840 645 537 537 274
240 138 129 80 51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2975 3007 3499 3531 5735 5767 8493 8525 2975
Size: 32 492 32 2204 32 2726 32 2983
Fragments arranged by size:
2983 2726 2204 492 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8533
XbaI T'CTAG_A
Cuts at: 5869 5869
Size: 8533
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2311 3406 3947 2311
Size: 1095 541 6897
Fragments arranged by size:
6897 1095 541
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8533
XmaI C'CCGG_G
Cuts at: 1870 2355 3379 3487 3547 3970 4278 5126 5858
Size: 485 1024 108 60 423 308 848 732
Cuts at: 5858 1870
Size: 4545
Fragments arranged by size:
4545 1024 848 732 485 423 308 108
60
XmnI GAAnn'nnTTC
Cuts at: 2235 3038 3125 3182 4880 5024 6662 2235
Size: 803 87 57 1698 144 1638 4106
Fragments arranged by size:
4106 1698 1638 803 144 87 57
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3823 6341 276
Size: 53 83 186 936 2289 2518 2468
Fragments arranged by size:
2518 2468 2289 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BplI BsmBI
BstZ17I EcoRV FseI FspAI NruI NsiI PacI PfoI
PspXI PsrI SacII SanDI SexAI SfiI SrfI SwaI