Restriction map for
pFN21AA1668
(Circular) (Six-base) MAPSORT of: pFN21AA1668.seq Check: 2971 from: 1 to: 7294
pFN21AA1668 7294 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3196 3563 3934 3991 3196
Size: 367 371 57 6499
Fragments arranged by size:
6499 371 367 57
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5104 278
Size: 53 83 186 936 3568 2468
Fragments arranged by size:
3568 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4637 1937
Size: 2700 4594
Acc65I G'GTAC_C
Cuts at: 3641 4615 3641
Size: 974 6320
AclI AA'CG_TT
Cuts at: 1563 5421 5794 1563
Size: 3858 373 3063
Fragments arranged by size:
3858 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5357 6741 7009 1632
Size: 3725 1384 268 1917
Fragments arranged by size:
3725 1917 1384 268
AfeI AGC'GCT
Cuts at: 2531 3528 2531
Size: 997 6297
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7097
AflIII A'CryG_T
Cuts at: 1203 6193 7170 1203
Size: 4990 977 1327
Fragments arranged by size:
4990 1327 977
AgeI A'CCGG_T
Cuts at: 6125 6125
Size: 7294
AhdI GACnn_n'nnGTC
Cuts at: 6023 6023
Size: 7294
AleI CACnn'nnGTG
Cuts at: 1166 2405 2639 3125 1166
Size: 1239 234 486 5335
Fragments arranged by size:
5335 1239 486 234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7294
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3033 3065 3033
Size: 32 7262
AlwNI CAG_nnn'CTG
Cuts at: 2798 2881 3623 4255 5037 6609 2798
Size: 83 742 632 782 1572 3483
Fragments arranged by size:
3483 1572 782 742 632 83
ApaI G_GGCC'C
Cuts at: 2672 2703 4342 2672
Size: 31 1639 5624
Fragments arranged by size:
5624 1639 31
ApaLI G'TGCA_C
Cuts at: 2516 5351 6507 2516
Size: 2835 1156 3303
Fragments arranged by size:
3303 2835 1156
ApoI r'AATT_y
Cuts at: 1493 4603 4829 4981 7090 1493
Size: 3110 226 152 2109 1697
Fragments arranged by size:
3110 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5848 160
Size: 872 4816 1606
Fragments arranged by size:
4816 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7294
AvaI C'yCGr_G
Cuts at: 1870 1943 2671 2856 3178 4341 4619 1870
Size: 73 728 185 322 1163 278 4545
Fragments arranged by size:
4545 1163 728 322 278 185 73
BamHI G'GATC_C
Cuts at: 2229 3962 4624 4991 2229
Size: 1733 662 367 4532
Fragments arranged by size:
4532 1733 662 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2037 2133 2468 2633 3221
Size: 325 217 627 250 96 335 165 588
Cuts at: 3221 3641 3800 3844 4293 4615 6070 618
Size: 420 159 44 449 322 1455 1842
Fragments arranged by size:
1842 1455 627 588 449 420 335 325
322 250 217 165 159 96 44
BanII G_rGCy'C
Cuts at: 1391 2442 2672 2703 3248 3614 3842 4230 4290
Size: 1051 230 31 545 366 228 388 60
Cuts at: 4290 4342 4613 1391
Size: 52 271 4072
Fragments arranged by size:
4072 1051 545 388 366 271 230 228
60 52 31
BbeI G_GCGC'C
Cuts at: 3804 3804
Size: 7294
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2173 4231 4446 928
Size: 971 274 2058 215 3776
Fragments arranged by size:
3776 2058 971 274 215
BbvCI CC'TCA_GC
Cuts at: 2285 2799 2285
Size: 514 6780
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5485 5519 1903
Size: 34 3548 34 3678
Fragments arranged by size:
3678 3548 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4063 5186 6396 4063
Size: 1123 1210 4961
Fragments arranged by size:
4961 1210 1123
BclI T'GATC_A
Cuts at: 1548 4461 1548
Size: 2913 4381
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3976 5905 136
Size: 107 122 71 631 605 2304 1929 1525
Fragments arranged by size:
2304 1929 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7289 7289
Size: 7294
BlpI GC'TnA_GC
Cuts at: 3530 4711 3530
Size: 1181 6113
Bme1580I G_kGCm'C
Cuts at: 1790 2471 2520 2636 2672 2703 3606 4342 5355
Size: 681 49 116 36 31 903 736 1013
Cuts at: 5355 6511 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1013 903 736 681 116 49
36 31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7294
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2302 5025 5983
Size: 416 53 83 399 24 885 2723 958
Cuts at: 5983 442
Size: 1753
Fragments arranged by size:
2723 1753 958 885 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7294
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2458 2461 3523 2458
Size: 3 1062 6229
Fragments arranged by size:
6229 1062 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2388 2572 2764 2956 3281 3303 3608 4118
Size: 1149 184 192 192 325 22 305 510
Cuts at: 4118 4139 4277 5954 1239
Size: 21 138 1677 2579
Fragments arranged by size:
2579 1677 1149 510 325 305 192 192
184 138 22 21
Bpu10I CC'TnA_GC
Cuts at: 2084 2285 2427 2799 3769 2084
Size: 201 142 372 970 5609
Fragments arranged by size:
5609 970 372 201 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3714 5419 6284 6582 3714
Size: 1705 865 298 4426
Fragments arranged by size:
4426 1705 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2266 5957 882
Size: 617 767 3691 2219
Fragments arranged by size:
3691 2219 767 617
BsaAI yAC'GTr
Cuts at: 493 1204 2493 5085 493
Size: 711 1289 2592 2702
Fragments arranged by size:
2702 2592 1289 711
BsaBI GATnn'nnATC
Cuts at: 7288 7288
Size: 7294
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3801 5101 5483 6118
Size: 53 83 186 936 2268 1300 382 635
Cuts at: 6118 275
Size: 1451
Fragments arranged by size:
2268 1451 1300 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2571 3912 4290 5727 6125 6399 6546 6957 2571
Size: 1341 378 1437 398 274 147 411 2908
Fragments arranged by size:
2908 1437 1341 411 398 378 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2246 2276 3456 3486 3530
Size: 30 165 30 843 30 1180 30 44
Cuts at: 3530 3560 1178
Size: 30 4912
Fragments arranged by size:
4912 1180 843 165 44 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2434 2450 2642 3242 3302 3305 3308 3311
Size: 1248 16 192 600 60 3 3 3
Cuts at: 3311 3604 3667 3679 4061 4136 4289 4343 1186
Size: 293 63 12 382 75 153 54 4137
Fragments arranged by size:
4137 1248 600 382 293 192 153 75
63 60 54 16 12 3 3 3
BseYI C'CCAG_C
Cuts at: 2009 2224 2483 2665 2774 2944 3237 3329 3452
Size: 215 259 182 109 170 293 92 123
Cuts at: 3452 3777 3937 6497 6993 2009
Size: 325 160 2560 496 2310
Fragments arranged by size:
2560 2310 496 325 293 259 215 182
170 160 123 109 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2111 2546 2667 3020 3149 3309 3368
Size: 573 375 435 121 353 129 160 59
Cuts at: 3368 3597 1163
Size: 229 4860
Fragments arranged by size:
4860 573 435 375 353 229 160 129
121 59
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2684 2837 4744 5505 6533 1540
Size: 275 186 683 153 1907 761 1028 2301
Fragments arranged by size:
2301 1907 1028 761 683 275 186 153
BsiHKAI G_wGCw'C
Cuts at: 2442 2520 2588 2788 2939 3614 4104 4230 4290
Size: 78 68 200 151 675 490 126 60
Cuts at: 4290 5355 5440 6511 2442
Size: 1065 85 1071 3225
Fragments arranged by size:
3225 1071 1065 675 490 200 151 126
85 78 68 60
BsmI GAATG_Cn'
Cuts at: 4805 4898 4805
Size: 93 7201
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2442 2471 2520 2588 2636 2672 2703
Size: 399 652 29 49 68 48 36 31
Cuts at: 2703 2788 2939 3248 3606 3614 3842 4104 4230
Size: 85 151 309 358 8 228 262 126
Cuts at: 4230 4290 4342 4613 5355 5440 6511 1391
Size: 60 52 271 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 652 399 358 309 271
262 228 151 126 85 85 68 60
52 49 48 36 31 29 8
BspEI T'CCGG_A
Cuts at: 6957 6957
Size: 7294
BspHI T'CATG_A
Cuts at: 2292 5183 6913 2292
Size: 2891 1730 2673
Fragments arranged by size:
2891 2673 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2154 3196 3563 3868 3934 3991 4649 844
Size: 1310 1042 367 305 66 57 658 3489
Fragments arranged by size:
3489 1310 1042 658 367 305 66 57
BsrBI CCG'CTC
Cuts at: 1394 1823 2237 2970 5181 1394
Size: 429 414 733 2211 3507
Fragments arranged by size:
3507 2211 733 429 414
BsrDI GCAATG_nn'
Cuts at: 65 1248 5789 5963 65
Size: 1183 4541 174 1396
Fragments arranged by size:
4541 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2130 2136 2471 4083 4419 5938 6125
Size: 110 320 6 335 1612 336 1519 187
Cuts at: 6125 1700
Size: 2869
Fragments arranged by size:
2869 1612 1519 336 335 320 187 110
6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7294
BssHII G'CGCG_C
Cuts at: 1925 2022 3445 1925
Size: 97 1423 5774
Fragments arranged by size:
5774 1423 97
BssSI C'ACGA_G
Cuts at: 4193 5354 6366 4193
Size: 1161 1012 5121
Fragments arranged by size:
5121 1161 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2493 2515 2524 2747 3043 794
Size: 1699 22 9 223 296 5045
Fragments arranged by size:
5045 1699 296 223 22 9
BstBI TT'CG_AA
Cuts at: 6098 6098
Size: 7294
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7294
BstXI CCAn_nnnn'nTGG
Cuts at: 2257 2407 3638 2257
Size: 150 1231 5913
Fragments arranged by size:
5913 1231 150
BstYI r'GATC_y
Cuts at: 1972 2073 2229 2841 3962 4624 4991 5376 5393
Size: 101 156 612 1121 662 367 385 17
Cuts at: 5393 6845 6931 6943 7289 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1121 662 612 385 367 346
156 101 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2446 2625 2826 4044 4497 2446
Size: 179 201 1218 453 5243
Fragments arranged by size:
5243 1218 453 201 179
BtgI C'CryG_G
Cuts at: 513 1065 2004 2015 2062 3113 4057 4105 513
Size: 552 939 11 47 1051 944 48 3702
Fragments arranged by size:
3702 1051 944 939 552 48 47 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2543 3504 487
Size: 683 56 1317 961 4277
Fragments arranged by size:
4277 1317 961 683 56
BtsI GCAGTG_nn'
Cuts at: 2563 3802 4810 5604 5624 2563
Size: 1239 1008 794 20 4233
Fragments arranged by size:
4233 1239 1008 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7294
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7259
DraI TTT'AAA
Cuts at: 4598 4598
Size: 7294
DraIII CAC_nnn'GTG
Cuts at: 2524 2524
Size: 7294
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2302 6301 809
Size: 507 986 3999 1802
Fragments arranged by size:
3999 1802 986 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2335 2473 2834
Size: 54 1425 50 275 18 505 138 361
Cuts at: 2834 2883 3252 3336 3510 4741 5630 7259 8
Size: 49 369 84 174 1231 889 1629 43
Fragments arranged by size:
1629 1425 1231 889 505 369 361 275
174 138 84 54 50 49 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2834 4741 1537
Size: 275 1022 1907 4090
Fragments arranged by size:
4090 1907 1022 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3306 4428 5224 1353
Size: 1953 1122 796 3423
Fragments arranged by size:
3423 1953 1122 796
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5881 6255 6401 387
Size: 1064 138 4292 374 146 1280
Fragments arranged by size:
4292 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2440 3612 4228 4288 2440
Size: 1172 616 60 5446
Fragments arranged by size:
5446 1172 616 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2388 2572 2764 2956 3281 3303 3608
Size: 393 756 184 192 192 325 22 305
Cuts at: 3608 4118 4139 4277 5357 5954 6741 7009 1239
Size: 510 21 138 1080 597 787 268 1524
Fragments arranged by size:
1524 1080 787 756 597 510 393 325
305 268 192 192 184 138 22 21
EcoNI CCTnn'n_nnAGG
Cuts at: 4145 4412 4145
Size: 267 7027
EcoO109I rG'GnC_Cy
Cuts at: 2365 2700 3106 3773 2365
Size: 335 406 667 5886
Fragments arranged by size:
5886 667 406 335
EcoRI G'AATT_C
Cuts at: 4603 4981 4603
Size: 378 6916
FspI TGC'GCA
Cuts at: 2497 5800 2497
Size: 3303 3991
HaeII r_GCGC'y
Cuts at: 1678 2533 3436 3530 3561 3804 4084 4267 6441
Size: 855 903 94 31 243 280 183 2174
Cuts at: 6441 1678
Size: 2531
Fragments arranged by size:
2531 2174 903 855 280 243 183 94
31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2221 2246 2253 2278 3530
Size: 32 135 32 649 25 7 25 1252
Cuts at: 3530 3562 5935 5967 6009 6041 1373
Size: 32 2373 32 42 32 2626
Fragments arranged by size:
2626 2373 1252 649 135 42 32 32
32 32 32 25 25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 4638 4884 669
Size: 988 281 2700 246 3079
Fragments arranged by size:
3079 2700 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7294
HpaI GTT'AAC
Cuts at: 4884 4884
Size: 7294
KasI G'GCGC_C
Cuts at: 3800 3800
Size: 7294
KpnI G_GTAC'C
Cuts at: 3645 4619 3645
Size: 974 6320
MfeI C'AATT_G
Cuts at: 4893 4893
Size: 7294
MluI A'CGCG_T
Cuts at: 7170 7170
Size: 7294
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3808 4431 6408 6592 1305
Size: 2503 623 1977 184 2007
Fragments arranged by size:
2503 2007 1977 623 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2337 2885 3338 10
Size: 54 1425 343 505 548 453 3966
Fragments arranged by size:
3966 1425 548 505 453 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2405 2639 3125 4169 4532 5252 5611
Size: 648 1239 234 486 1044 363 720 359
Cuts at: 5611 5770 518
Size: 159 2042
Fragments arranged by size:
2042 1239 1044 720 648 486 363 359
234 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2064 3323 3599 3874 4710 5387 6535
Size: 411 47 1259 276 275 836 677 1148
Cuts at: 6535 6780 1606
Size: 245 2120
Fragments arranged by size:
2120 1259 1148 836 677 411 276 275
245 47
NaeI GCC'GGC
Cuts at: 4085 4085
Size: 7294
NarI GG'CG_CC
Cuts at: 3801 3801
Size: 7294
NcoI C'CATG_G
Cuts at: 513 1065 2004 3113 4057 513
Size: 552 939 1109 944 3750
Fragments arranged by size:
3750 1109 944 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7294
NgoMIV G'CCGG_C
Cuts at: 4083 4083
Size: 7294
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7294
NotI GC'GGCC_GC
Cuts at: 4741 4741
Size: 7294
NspI r_CATG'y
Cuts at: 1132 1229 2516 4168 4652 6197 1132
Size: 97 1287 1652 484 1545 2229
Fragments arranged by size:
2229 1652 1545 1287 484 97
PasI CC'CwG_GG
Cuts at: 1348 2603 3352 1348
Size: 1255 749 5290
Fragments arranged by size:
5290 1255 749
PciI A'CATG_T
Cuts at: 6193 6193
Size: 7294
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7294
PfoI T'CCnGG_A
Cuts at: 2097 2241 2761 2097
Size: 144 520 6630
Fragments arranged by size:
6630 520 144
PmeI GTTT'AAAC
Cuts at: 4598 4598
Size: 7294
PmlI CAC'GTG
Cuts at: 2493 2493
Size: 7294
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5363 5395 6876 6908 5363
Size: 32 1481 32 5749
Fragments arranged by size:
5749 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3106 3773 3106
Size: 667 6627
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7294
PsiI TTA'TAA
Cuts at: 4864 4864
Size: 7294
PspOMI G'GGCC_C
Cuts at: 2668 2699 4338 2668
Size: 31 1639 5624
Fragments arranged by size:
5624 1639 31
PstI C_TGCA'G
Cuts at: 830 2330 2794 2982 3574 3797 3879 3931 4646
Size: 1500 464 188 592 223 82 52 715
Cuts at: 4646 830
Size: 3478
Fragments arranged by size:
3478 1500 715 592 464 223 188 82
52
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7294
PvuII CAG'CTG
Cuts at: 3323 3599 3874 3323
Size: 276 275 6743
Fragments arranged by size:
6743 276 275
SacI G_AGCT'C
Cuts at: 2442 3614 4230 4290 2442
Size: 1172 616 60 5446
Fragments arranged by size:
5446 1172 616 60
SacII CC_GC'GG
Cuts at: 2018 2065 2018
Size: 47 7247
SalI G'TCGA_C
Cuts at: 1936 4636 1936
Size: 2700 4594
SbfI CC_TGCA'GG
Cuts at: 2794 4646 2794
Size: 1852 5442
ScaI AGT'ACT
Cuts at: 5542 7254 5542
Size: 1712 5582
SexAI A'CCwGG_T
Cuts at: 2991 2991
Size: 7294
SfcI C'TryA_G
Cuts at: 826 1045 2326 2391 2790 2873 2978 3570 3793
Size: 219 1281 65 399 83 105 592 223
Cuts at: 3793 3875 3927 4642 5777 6458 6649 826
Size: 82 52 715 1135 681 191 1471
Fragments arranged by size:
1471 1281 1135 715 681 592 399 223
219 191 105 83 82 65 52
SfoI GGC'GCC
Cuts at: 3802 3802
Size: 7294
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7294
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7294
SmaI CCC'GGG
Cuts at: 1872 2673 2858 3180 4343 4621 1872
Size: 801 185 322 1163 278 4545
Fragments arranged by size:
4545 1163 801 322 278 185
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3729 5398 6299 6561 820
Size: 197 926 1786 1669 901 262 1553
Fragments arranged by size:
1786 1669 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5085 493
Size: 4592 2702
SpeI A'CTAG_T
Cuts at: 152 3700 152
Size: 3548 3746
SphI G_CATG'C
Cuts at: 1132 4652 1132
Size: 3520 3774
SspI AAT'ATT
Cuts at: 5 52 5218 5
Size: 47 5166 2081
Fragments arranged by size:
5166 2081 47
StuI AGG'CCT
Cuts at: 1517 2695 2927 1517
Size: 1178 232 5884
Fragments arranged by size:
5884 1178 232
StyI C'CwwG_G
Cuts at: 513 1065 2004 2169 2751 2814 3113 3957 4057
Size: 552 939 165 582 63 299 844 100
Cuts at: 4057 4733 513
Size: 676 3074
Fragments arranged by size:
3074 939 844 676 582 552 299 165
100 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2371 2413 3343 3388 3466 5321 5338
Size: 1255 791 42 930 45 78 1855 17
Cuts at: 5338 5491 5676 6121 325
Size: 153 185 445 1498
Fragments arranged by size:
1855 1498 1255 930 791 445 185 153
78 45 42 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4035 5540 7252
Size: 274 80 33 51 1443 2058 1505 1712
Cuts at: 7252 96
Size: 138
Fragments arranged by size:
2058 1712 1505 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3350 3382 3456 3488 7254 7286 3350
Size: 32 74 32 3766 32 3358
Fragments arranged by size:
3766 3358 74 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2177 2258 2680 1316
Size: 861 81 422 5930
Fragments arranged by size:
5930 861 422 81
XbaI T'CTAG_A
Cuts at: 4630 4630
Size: 7294
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3349 3679 4317 3349
Size: 330 638 6326
Fragments arranged by size:
6326 638 330
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7294
XmaI C'CCGG_G
Cuts at: 1870 2671 2856 3178 4341 4619 1870
Size: 801 185 322 1163 278 4545
Fragments arranged by size:
4545 1163 801 322 278 185
XmnI GAAnn'nnTTC
Cuts at: 4475 5423 4475
Size: 948 6346
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5102 276
Size: 53 83 186 936 3568 2468
Fragments arranged by size:
3568 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV
FalI FseI FspAI NruI NsiI PacI PspXI PsrI
RsrII SanDI SapI SfiI SrfI SwaI