Restriction map for
pFN21AA1667
(Circular) (Six-base) MAPSORT of: pFN21AA1667.seq Check: 4862 from: 1 to: 6829
pFN21AA1667 6829 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3853 4036 3853
Size: 183 6646
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4639 278
Size: 53 83 186 936 3103 2468
Fragments arranged by size:
3103 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4172 1937
Size: 2235 4594
Acc65I G'GTAC_C
Cuts at: 4150 4150
Size: 6829
AclI AA'CG_TT
Cuts at: 1563 4956 5329 1563
Size: 3393 373 3063
Fragments arranged by size:
3393 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2264 2546 4057 4127 4892 6276 6544 1632
Size: 632 282 1511 70 765 1384 268 1917
Fragments arranged by size:
1917 1511 1384 765 632 282 268 70
AfeI AGC'GCT
Cuts at: 2558 2558
Size: 6829
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6632
AflIII A'CryG_T
Cuts at: 1203 3175 5728 6705 1203
Size: 1972 2553 977 1327
Fragments arranged by size:
2553 1972 1327 977
AgeI A'CCGG_T
Cuts at: 5660 5660
Size: 6829
AhdI GACnn_n'nnGTC
Cuts at: 2603 5558 2603
Size: 2955 3874
AleI CACnn'nnGTG
Cuts at: 1166 3974 1166
Size: 2808 4021
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3051 3622 1184
Size: 1867 571 4391
Fragments arranged by size:
4391 1867 571
AlwNI CAG_nnn'CTG
Cuts at: 2140 3515 4021 4102 4572 6144 2140
Size: 1375 506 81 470 1572 2825
Fragments arranged by size:
2825 1572 1375 506 470 81
ApaLI G'TGCA_C
Cuts at: 4886 6042 4886
Size: 1156 5673
ApoI r'AATT_y
Cuts at: 1493 2439 3920 4138 4364 4516 6625 1493
Size: 946 1481 218 226 152 2109 1697
Fragments arranged by size:
2109 1697 1481 946 226 218 152
AseI AT'TA_AT
Cuts at: 160 1032 5383 160
Size: 872 4351 1606
Fragments arranged by size:
4351 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6829
AvaI C'yCGr_G
Cuts at: 1870 1943 4154 1870
Size: 73 2211 4545
Fragments arranged by size:
4545 2211 73
BamHI G'GATC_C
Cuts at: 4159 4526 4159
Size: 367 6462
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4024 4067 4150 5605 618
Size: 325 217 627 2237 43 83 1455 1842
Fragments arranged by size:
2237 1842 1455 627 325 217 83 43
BanII G_rGCy'C
Cuts at: 1391 2304 2514 3202 3322 3618 4043 4148 1391
Size: 913 210 688 120 296 425 105 4072
Fragments arranged by size:
4072 913 688 425 296 210 120 105
BbeI G_GCGC'C
Cuts at: 4071 4071
Size: 6829
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3342 928
Size: 971 1443 4415
Fragments arranged by size:
4415 1443 971
BbvCI CC'TCA_GC
Cuts at: 3059 3675 3059
Size: 616 6213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3598 3632 5020 5054 1903
Size: 34 1661 34 1388 34 3678
Fragments arranged by size:
3678 1661 1388 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4721 5931 4721
Size: 1210 5619
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6829
BfrBI ATG'CAT
Cuts at: 3913 3913
Size: 6829
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3971 4083 5440
Size: 107 122 71 631 605 2299 112 1357
Cuts at: 5440 136
Size: 1525
Fragments arranged by size:
2299 1525 1357 631 605 122 112 107
71
BglII A'GATC_T
Cuts at: 6824 6824
Size: 6829
BlpI GC'TnA_GC
Cuts at: 3538 4246 3538
Size: 708 6121
Bme1580I G_kGCm'C
Cuts at: 1790 4890 6046 1790
Size: 3100 1156 2573
Fragments arranged by size:
3100 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6829
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4560 5518 442
Size: 416 53 83 399 24 3143 958 1753
Fragments arranged by size:
3143 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6829
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3808 3808
Size: 6829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2199 2805 2816 3023 3214 3568 3878 5489
Size: 960 606 11 207 191 354 310 1611
Cuts at: 5489 1239
Size: 2579
Fragments arranged by size:
2579 1611 960 606 354 310 207 191
11
Bpu10I CC'TnA_GC
Cuts at: 3059 3135 3675 3059
Size: 76 540 6213
Fragments arranged by size:
6213 540 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4954 5819 6117 4954
Size: 865 298 5666
Fragments arranged by size:
5666 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2814 5492 882
Size: 617 1315 2678 2219
Fragments arranged by size:
2678 2219 1315 617
BsaAI yAC'GTr
Cuts at: 493 1204 4620 493
Size: 711 3416 2702
Fragments arranged by size:
3416 2702 711
BsaBI GATnn'nnATC
Cuts at: 6823 6823
Size: 6829
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4068 4636 5018 5653
Size: 53 83 186 936 2535 568 382 635
Cuts at: 5653 275
Size: 1451
Fragments arranged by size:
2535 1451 936 635 568 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5262 5660 5934 6081 6492 5262
Size: 398 274 147 411 5599
Fragments arranged by size:
5599 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2501 2531 1178
Size: 30 165 30 1098 30 5476
Fragments arranged by size:
5476 1098 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2205 3055 3692 1186
Size: 1019 850 637 4323
Fragments arranged by size:
4323 1019 850 637
BseYI C'CCAG_C
Cuts at: 2284 2847 3309 3688 3927 6032 6528 2284
Size: 563 462 379 239 2105 496 2585
Fragments arranged by size:
2585 2105 563 496 462 379 239
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3011 1163
Size: 573 1275 4981
Fragments arranged by size:
4981 1275 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3829 4279 5040 6068 1540
Size: 275 186 1828 450 761 1028 2301
Fragments arranged by size:
2301 1828 1028 761 450 275 186
BsiHKAI G_wGCw'C
Cuts at: 2304 2414 2873 4043 4890 4975 6046 2304
Size: 110 459 1170 847 85 1071 3087
Fragments arranged by size:
3087 1170 1071 847 459 110 85
BsmI GAATG_Cn'
Cuts at: 2538 3530 3831 4340 4433 2538
Size: 992 301 509 93 4934
Fragments arranged by size:
4934 992 509 301 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3761 3761
Size: 6829
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 2414 2514 2873 3202 3322 3618
Size: 399 514 110 100 359 329 120 296
Cuts at: 3618 4043 4148 4890 4975 6046 1391
Size: 425 105 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 514 425 399 359 329
296 120 110 105 100 85
BspEI T'CCGG_A
Cuts at: 6492 6492
Size: 6829
BspHI T'CATG_A
Cuts at: 4718 6448 4718
Size: 1730 5099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2558 3319 3853 3864 4036 4184 844
Size: 1714 761 534 11 172 148 3489
Fragments arranged by size:
3489 1714 761 534 172 148 11
BsrBI CCG'CTC
Cuts at: 1394 1823 2714 4716 1394
Size: 429 891 2002 3507
Fragments arranged by size:
3507 2002 891 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2717 5324 5498 65
Size: 1183 1469 2607 174 1396
Fragments arranged by size:
2607 1469 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5473 5660 1700
Size: 110 3663 187 2869
Fragments arranged by size:
3663 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6829
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6829
BssSI C'ACGA_G
Cuts at: 2041 2780 3617 3796 4889 5901 2041
Size: 739 837 179 1093 1012 2969
Fragments arranged by size:
2969 1093 1012 837 739 179
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4102 794
Size: 3308 3521
BstBI TT'CG_AA
Cuts at: 5633 5633
Size: 6829
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6829
BstXI CCAn_nnnn'nTGG
Cuts at: 3238 3238
Size: 6829
BstYI r'GATC_y
Cuts at: 1972 2932 3427 4159 4526 4911 4928 6380 6466
Size: 960 495 732 367 385 17 1452 86
Cuts at: 6466 6478 6824 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 960 732 495 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2664 3154 3255 4059 2664
Size: 490 101 804 5434
Fragments arranged by size:
5434 804 490 101
BtgI C'CryG_G
Cuts at: 513 1065 2004 2913 3456 3969 513
Size: 552 939 909 543 513 3373
Fragments arranged by size:
3373 939 909 552 543 513
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6090
Fragments arranged by size:
6090 683 56
BtsI GCAGTG_nn'
Cuts at: 3640 4345 5139 5159 3640
Size: 705 794 20 5310
Fragments arranged by size:
5310 794 705 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6829
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6794
DraI TTT'AAA
Cuts at: 4133 4133
Size: 6829
DraIII CAC_nnn'GTG
Cuts at: 2418 4117 2418
Size: 1699 5130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5836 809
Size: 507 4520 1802
Fragments arranged by size:
4520 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2899 2992
Size: 54 1425 50 275 18 177 892 93
Cuts at: 2992 3346 3867 4276 5165 6794 8
Size: 354 521 409 889 1629 43
Fragments arranged by size:
1629 1425 892 889 521 409 354 275
177 93 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4276 1537
Size: 275 2464 4090
Fragments arranged by size:
4090 2464 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4759 1353
Size: 3406 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5416 5790 5936 387
Size: 1064 138 3827 374 146 1280
Fragments arranged by size:
3827 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2302 4041 2302
Size: 1739 5090
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 2264 2546 2805 2816 3023 3214
Size: 393 567 65 282 259 11 207 191
Cuts at: 3214 3568 3878 4057 4127 4892 5489 6276 6544
Size: 354 310 179 70 765 597 787 268
Cuts at: 6544 1239
Size: 1524
Fragments arranged by size:
1524 787 765 597 567 393 354 310
282 268 259 207 191 179 70 65
11
EcoNI CCTnn'n_nnAGG
Cuts at: 2685 3074 3476 3860 2685
Size: 389 402 384 5654
Fragments arranged by size:
5654 402 389 384
EcoO109I rG'GnC_Cy
Cuts at: 2641 3396 3405 2641
Size: 755 9 6065
Fragments arranged by size:
6065 755 9
EcoRI G'AATT_C
Cuts at: 4138 4516 4138
Size: 378 6451
FspI TGC'GCA
Cuts at: 5335 5335
Size: 6829
HaeII r_GCGC'y
Cuts at: 1678 2560 3228 4071 5976 1678
Size: 882 668 843 1905 2531
Fragments arranged by size:
2531 1905 882 843 668
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2018 2050 2589 2621 2794
Size: 32 135 32 446 32 539 32 173
Cuts at: 2794 2826 2924 2956 3613 3645 5470 5502 5544
Size: 32 98 32 657 32 1825 32 42
Cuts at: 5544 5576 1373
Size: 32 2626
Fragments arranged by size:
2626 1825 657 539 446 173 135 98
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4173 4419 669
Size: 988 281 2235 246 3079
Fragments arranged by size:
3079 2235 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2952 3449 748
Size: 2204 497 4128
Fragments arranged by size:
4128 2204 497
HpaI GTT'AAC
Cuts at: 4419 4419
Size: 6829
KasI G'GCGC_C
Cuts at: 4067 4067
Size: 6829
KpnI G_GTAC'C
Cuts at: 4154 4154
Size: 6829
MfeI C'AATT_G
Cuts at: 4428 4428
Size: 6829
MluI A'CGCG_T
Cuts at: 6705 6705
Size: 6829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2123 2323 5943 6127 1305
Size: 818 200 3620 184 2007
Fragments arranged by size:
3620 2007 818 200 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2901 2994 3348 3869
Size: 54 1425 343 177 892 93 354 521
Cuts at: 3869 10
Size: 2970
Fragments arranged by size:
2970 1425 892 521 354 343 177 93
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3974 4787 5146 5305 518
Size: 648 2808 813 359 159 2042
Fragments arranged by size:
2808 2042 813 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2190 2316 3685 3931 4021 4245 4922 6070
Size: 584 126 1369 246 90 224 677 1148
Cuts at: 6070 6315 1606
Size: 245 2120
Fragments arranged by size:
2120 1369 1148 677 584 246 245 224
126 90
NarI GG'CG_CC
Cuts at: 4068 4068
Size: 6829
NcoI C'CATG_G
Cuts at: 513 1065 2004 2913 513
Size: 552 939 909 4429
Fragments arranged by size:
4429 939 909 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6829
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6829
NotI GC'GGCC_GC
Cuts at: 4276 4276
Size: 6829
NsiI A_TGCA'T
Cuts at: 3915 3915
Size: 6829
NspI r_CATG'y
Cuts at: 1132 1229 3179 4187 5732 1132
Size: 97 1950 1008 1545 2229
Fragments arranged by size:
2229 1950 1545 1008 97
PasI CC'CwG_GG
Cuts at: 1348 3070 3777 1348
Size: 1722 707 4400
Fragments arranged by size:
4400 1722 707
PciI A'CATG_T
Cuts at: 3175 5728 3175
Size: 2553 4276
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2864 2919 3001 4021 1108
Size: 1756 55 82 1020 3916
Fragments arranged by size:
3916 1756 1020 82 55
PfoI T'CCnGG_A
Cuts at: 2832 3430 3999 2832
Size: 598 569 5662
Fragments arranged by size:
5662 598 569
PmeI GTTT'AAAC
Cuts at: 4133 4133
Size: 6829
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2647 2679 4898 4930 6411 6443 2647
Size: 32 2219 32 1481 32 3033
Fragments arranged by size:
3033 2219 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2641 3396 2641
Size: 755 6074
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6829
PsiI TTA'TAA
Cuts at: 4399 4399
Size: 6829
PstI C_TGCA'G
Cuts at: 830 2547 3571 4033 4181 830
Size: 1717 1024 462 148 3478
Fragments arranged by size:
3478 1717 1024 462 148
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6829
PvuII CAG'CTG
Cuts at: 2316 3931 4021 2316
Size: 1615 90 5124
Fragments arranged by size:
5124 1615 90
SacI G_AGCT'C
Cuts at: 2304 4043 2304
Size: 1739 5090
SalI G'TCGA_C
Cuts at: 1936 4171 1936
Size: 2235 4594
SbfI CC_TGCA'GG
Cuts at: 4181 4181
Size: 6829
ScaI AGT'ACT
Cuts at: 5077 6789 5077
Size: 1712 5117
SfcI C'TryA_G
Cuts at: 826 1045 2543 3567 4029 4177 5312 5993 6184
Size: 219 1498 1024 462 148 1135 681 191
Cuts at: 6184 826
Size: 1471
Fragments arranged by size:
1498 1471 1135 1024 681 462 219 191
148
SfoI GGC'GCC
Cuts at: 4069 4069
Size: 6829
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6829
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6829
SmaI CCC'GGG
Cuts at: 1872 4156 1872
Size: 2284 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4933 5834 6096 820
Size: 197 926 2990 901 262 1553
Fragments arranged by size:
2990 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4620 493
Size: 4127 2702
SpeI A'CTAG_T
Cuts at: 152 3082 152
Size: 2930 3899
SphI G_CATG'C
Cuts at: 1132 4187 1132
Size: 3055 3774
SspI AAT'ATT
Cuts at: 5 52 4753 5
Size: 47 4701 2081
Fragments arranged by size:
4701 2081 47
StuI AGG'CCT
Cuts at: 1517 3471 1517
Size: 1954 4875
StyI C'CwwG_G
Cuts at: 513 1065 2004 2076 2637 2913 4268 513
Size: 552 939 72 561 276 1355 3074
Fragments arranged by size:
3074 1355 939 561 552 276 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2269 2627 4856 4873 5026 5211 5656
Size: 1255 689 358 2229 17 153 185 445
Cuts at: 5656 325
Size: 1498
Fragments arranged by size:
2229 1498 1255 689 445 358 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5075 6787 96
Size: 274 80 33 51 1443 3098 1712 138
Fragments arranged by size:
3098 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2917 2949 6789 6821 2917
Size: 32 3840 32 2925
Fragments arranged by size:
3840 2925 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2806 1316
Size: 1490 5339
XbaI T'CTAG_A
Cuts at: 4165 4165
Size: 6829
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2861 2902 3512 2861
Size: 41 610 6178
Fragments arranged by size:
6178 610 41
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6829
XmaI C'CCGG_G
Cuts at: 1870 4154 1870
Size: 2284 4545
XmnI GAAnn'nnTTC
Cuts at: 4958 4958
Size: 6829
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4637 276
Size: 53 83 186 936 3103 2468
Fragments arranged by size:
3103 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BsiWI BstZ17I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI