Restriction map for
pFN21AA1662
(Circular) (Six-base) MAPSORT of: pFN21AA1662.seq Check: 795 from: 1 to: 11800
pFN21AA1662 11800 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2185 5194 6055 8365 8602 9048 2185
Size: 3009 861 2310 237 446 4937
Fragments arranged by size:
4937 3009 2310 861 446 237
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8976 9610 278
Size: 53 83 186 936 7440 634 2468
Fragments arranged by size:
7440 2468 936 634 186 83 53
AccI GT'mk_AC
Cuts at: 1937 9143 1937
Size: 7206 4594
Acc65I G'GTAC_C
Cuts at: 5349 9121 5349
Size: 3772 8028
AclI AA'CG_TT
Cuts at: 1563 9927 10300 1563
Size: 8364 373 3063
Fragments arranged by size:
8364 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2118 5306 7656 7772 8618 9050 9863 11247
Size: 486 3188 2350 116 846 432 813 1384
Cuts at: 11247 11515 1632
Size: 268 1917
Fragments arranged by size:
3188 2350 1917 1384 846 813 486 432
268 116
AfeI AGC'GCT
Cuts at: 7949 8948 7949
Size: 999 10801
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11603
AflIII A'CryG_T
Cuts at: 1203 2819 10699 11676 1203
Size: 1616 7880 977 1327
Fragments arranged by size:
7880 1616 1327 977
AgeI A'CCGG_T
Cuts at: 2712 10631 2712
Size: 7919 3881
AhdI GACnn_n'nnGTC
Cuts at: 7662 10529 7662
Size: 2867 8933
AleI CACnn'nnGTG
Cuts at: 1166 5338 5492 6611 1166
Size: 4172 154 1119 6355
Fragments arranged by size:
6355 4172 1119 154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5085 5529 5685 1184
Size: 3901 444 156 7299
Fragments arranged by size:
7299 3901 444 156
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3244 3276 3391 3423 3538 3570 3685 3717 3832
Size: 32 115 32 115 32 115 32 115
Cuts at: 3832 3864 3244
Size: 32 11180
Fragments arranged by size:
11180 115 115 115 115 32 32 32
32 32
AlwNI CAG_nnn'CTG
Cuts at: 2141 6274 8101 8422 8722 8743 9543 11115 2141
Size: 4133 1827 321 300 21 800 1572 2826
Fragments arranged by size:
4133 2826 1827 1572 800 321 300 21
ApaI G_GGCC'C
Cuts at: 2253 2264 5871 6320 6371 6730 7061 7091 7748
Size: 11 3607 449 51 359 331 30 657
Cuts at: 7748 7882 8184 8236 8555 2253
Size: 134 302 52 319 5498
Fragments arranged by size:
5498 3607 657 449 359 331 319 302
134 52 51 30 11
ApaLI G'TGCA_C
Cuts at: 8277 9857 11013 8277
Size: 1580 1156 9064
Fragments arranged by size:
9064 1580 1156
ApoI r'AATT_y
Cuts at: 1493 3205 4684 9109 9335 9487 11596 1493
Size: 1712 1479 4425 226 152 2109 1697
Fragments arranged by size:
4425 2109 1712 1697 1479 226 152
AseI AT'TA_AT
Cuts at: 160 1032 10354 160
Size: 872 9322 1606
Fragments arranged by size:
9322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11800
AvaI C'yCGr_G
Cuts at: 1870 1943 2527 2538 4926 5076 5217 5364 5676
Size: 73 584 11 2388 150 141 147 312
Cuts at: 5676 5760 5777 6668 7262 8311 8956 9125 1870
Size: 84 17 891 594 1049 645 169 4545
Fragments arranged by size:
4545 2388 1049 891 645 594 584 312
169 150 147 141 84 73 17 11
AvrII C'CTAG_G
Cuts at: 2963 2963
Size: 11800
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4973 5006 8870 8903 4973
Size: 33 3864 33 7870
Fragments arranged by size:
7870 3864 33 33
BamHI G'GATC_C
Cuts at: 5572 9130 9497 5572
Size: 3558 367 7875
Fragments arranged by size:
7875 3558 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 2146 2709 5061 5349
Size: 325 217 627 227 132 563 2352 288
Cuts at: 5349 5505 5661 5725 6706 8002 8036 8134 8397
Size: 156 156 64 981 1296 34 98 263
Cuts at: 8397 9121 10576 618
Size: 724 1455 1842
Fragments arranged by size:
2352 1842 1455 1296 981 724 627 563
325 288 263 227 217 156 156 132
98 64 34
BanII G_rGCy'C
Cuts at: 1391 2124 2132 2253 2264 2283 2514 2575 5011
Size: 733 8 121 11 19 231 61 2436
Cuts at: 5011 5031 5136 5319 5759 5768 5871 6320 6371
Size: 20 105 183 440 9 103 449 51
Cuts at: 6371 6399 6510 6548 6730 6768 6956 7061 7091
Size: 28 111 38 182 38 188 105 30
Cuts at: 7091 7226 7748 7778 7848 7882 8184 8236 8555
Size: 135 522 30 70 34 302 52 319
Cuts at: 8555 8925 9119 1391
Size: 370 194 4072
Fragments arranged by size:
4072 2436 733 522 449 440 370 319
302 231 194 188 183 182 135 121
111 105 105 103 70 61 52 51
38 38 34 30 30 28 20 19
11 9 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 6043 928
Size: 971 4144 6685
Fragments arranged by size:
6685 4144 971
BbvCI CC'TCA_GC
Cuts at: 2142 2272 7485 8451 8473 8673 9096 2142
Size: 130 5213 966 22 200 423 4846
Fragments arranged by size:
5213 4846 966 423 200 130 22
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 9991 10025 1903
Size: 34 8054 34 3678
Fragments arranged by size:
8054 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9692 10902 9692
Size: 1210 10590
BclI T'GATC_A
Cuts at: 1548 1548
Size: 11800
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5006 5219 5978
Size: 107 122 71 631 605 3334 213 759
Cuts at: 5978 6089 6258 6375 7797 8559 10411 136
Size: 111 169 117 1422 762 1852 1525
Fragments arranged by size:
3334 1852 1525 1422 762 759 631 605
213 169 122 117 111 107 71
BglII A'GATC_T
Cuts at: 11795 11795
Size: 11800
BlpI GC'TnA_GC
Cuts at: 5767 8513 8525 8987 9217 5767
Size: 2746 12 462 230 8350
Fragments arranged by size:
8350 2746 462 230 12
Bme1580I G_kGCm'C
Cuts at: 1790 2151 2253 2264 3226 3373 3451 3520 3667
Size: 361 102 11 962 147 78 69 147
Cuts at: 3667 3745 3814 3892 3964 4036 4108 4180 4252
Size: 78 69 78 72 72 72 72 72
Cuts at: 4252 4393 5064 5477 5508 5664 5871 6320 6371
Size: 141 671 413 31 156 207 449 51
Cuts at: 6371 6443 6709 6730 7061 7091 7748 7882 8041
Size: 72 266 21 331 30 657 134 159
Cuts at: 8041 8139 8184 8236 8281 8555 8659 9861 11017
Size: 98 45 52 45 274 104 1202 1156
Cuts at: 11017 1790
Size: 2573
Fragments arranged by size:
2573 1202 1156 962 671 657 449 413
361 331 274 266 207 159 156 147
147 141 134 104 102 98 78 78
78 72 72 72 72 72 72 69
69 52 51 45 45 31 30 21
11
BmgBI CAC'GTC
Cuts at: 1318 2772 1318
Size: 1454 10346
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2473 2677 4882
Size: 416 53 83 399 24 1056 204 2205
Cuts at: 4882 4903 5627 5700 6568 6757 6911 6985 9531
Size: 21 724 73 868 189 154 74 2546
Cuts at: 9531 10489 442
Size: 958 1753
Fragments arranged by size:
2546 2205 1753 1056 958 868 724 416
399 204 189 154 83 74 73 53
24 21
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11800
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2553 4550 7405 8647 2553
Size: 1997 2855 1242 5706
Fragments arranged by size:
5706 2855 1997 1242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2369 2395 2723 2857 2887 2917 3232 3379
Size: 1130 26 328 134 30 30 315 147
Cuts at: 3379 3526 3673 3820 6155 6263 6364 6700 7067
Size: 147 147 147 2335 108 101 336 367
Cuts at: 7067 7398 7949 8123 8228 8261 8528 8666 8867
Size: 331 551 174 105 33 267 138 201
Cuts at: 8867 8909 9047 10460 1239
Size: 42 138 1413 2579
Fragments arranged by size:
2579 2335 1413 1130 551 367 336 331
328 315 267 201 174 147 147 147
147 138 138 134 108 105 101 42
33 30 30 26
Bpu10I CC'TnA_GC
Cuts at: 2123 2142 2272 2805 5025 5550 6870 7485 8451
Size: 19 130 533 2220 525 1320 615 966
Cuts at: 8451 8473 8673 9096 2123
Size: 22 200 423 4827
Fragments arranged by size:
4827 2220 1320 966 615 533 525 423
200 130 22 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2351 7214 9925 10790 11088 2351
Size: 4863 2711 865 298 3063
Fragments arranged by size:
4863 3063 2711 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4633 5777 6142 6229 10463 882
Size: 617 3134 1144 365 87 4234 2219
Fragments arranged by size:
4234 3134 2219 1144 617 365 87
BsaAI yAC'GTr
Cuts at: 493 1204 2822 7840 9591 493
Size: 711 1618 5018 1751 2702
Fragments arranged by size:
5018 2702 1751 1618 711
BsaBI GATnn'nnATC
Cuts at: 7361 11794 7361
Size: 4433 7367
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5222 8251 8973 9607
Size: 53 83 186 936 3689 3029 722 634
Cuts at: 9607 9989 10624 275
Size: 382 635 1451
Fragments arranged by size:
3689 3029 1451 936 722 635 634 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2712 8565 10233 10631 10905 11052 11463 2712
Size: 5853 1668 398 274 147 411 3049
Fragments arranged by size:
5853 3049 1668 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2393 2423 3103 3133 3244
Size: 30 165 30 990 30 680 30 111
Cuts at: 3244 3274 3391 3421 3538 3568 3685 3715 3832
Size: 30 117 30 117 30 117 30 117
Cuts at: 3832 3862 4333 4363 4840 4870 6705 6735 7460
Size: 30 471 30 477 30 1835 30 725
Cuts at: 7460 7490 8423 8453 8526 8556 8891 8921 9059
Size: 30 933 30 73 30 335 30 138
Cuts at: 9059 9089 1178
Size: 30 3889
Fragments arranged by size:
3889 1835 990 933 725 680 477 471
335 165 138 117 117 117 117 111
73 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2108 2501 2504 2545 2573 2619 2830
Size: 838 84 393 3 41 28 46 211
Cuts at: 2830 3166 3247 3316 3394 3463 3541 3610 3688
Size: 336 81 69 78 69 78 69 78
Cuts at: 3688 3757 3835 3904 3976 4048 4120 4192 4264
Size: 69 78 69 72 72 72 72 72
Cuts at: 4264 4336 4405 4594 5362 5674 5822 6503 7136
Size: 72 69 189 768 312 148 681 633
Cuts at: 7136 7145 7502 7937 7970 8090 8259 8399 8468
Size: 9 357 435 33 120 169 140 69
Cuts at: 8468 8690 8777 1186
Size: 222 87 4209
Fragments arranged by size:
4209 838 768 681 633 435 393 357
336 312 222 211 189 169 148 140
120 87 84 81 78 78 78 78
72 72 72 72 72 72 69 69
69 69 69 69 69 46 41 33
28 9 3
BseYI C'CCAG_C
Cuts at: 2498 2502 3081 3123 3225 3303 3372 3450 3519
Size: 4 579 42 102 78 69 78 69
Cuts at: 3519 3597 3666 3744 3813 3891 4064 4251 4392
Size: 78 69 78 69 78 173 187 141
Cuts at: 4392 4859 4872 5034 6008 6056 6398 6465 6618
Size: 467 13 162 974 48 342 67 153
Cuts at: 6618 6737 6796 6841 6900 6968 7091 7217 7888
Size: 119 59 45 59 68 123 126 671
Cuts at: 7888 7944 8235 11003 11499 2498
Size: 56 291 2768 496 2799
Fragments arranged by size:
2799 2768 974 671 579 496 467 342
291 187 173 162 153 141 126 123
119 102 78 78 78 78 78 69
69 69 69 68 67 59 59 56
48 45 42 13 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3327 3621 4603 5316 5450 5902 5937
Size: 573 1591 294 982 713 134 452 35
Cuts at: 5937 6964 7513 7574 7706 8924 1163
Size: 1027 549 61 132 1218 4039
Fragments arranged by size:
4039 1591 1218 1027 982 713 573 549
452 294 134 132 61 35
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7029 9250 10011 11039 1540
Size: 275 186 5028 2221 761 1028 2301
Fragments arranged by size:
5028 2301 2221 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 2514 4967 6510 6768 7079 7848 8281 8688
Size: 390 2453 1543 258 311 769 433 407
Cuts at: 8688 8925 9039 9861 9946 11017 2124
Size: 237 114 822 85 1071 2907
Fragments arranged by size:
2907 2453 1543 1071 822 769 433 407
390 311 258 237 114 85
BsiWI C'GTAC_G
Cuts at: 7660 7660
Size: 11800
BsmI GAATG_Cn'
Cuts at: 9311 9404 9311
Size: 93 11707
BsmBI CGTCTCn'nnnn_
Cuts at: 2583 2778 8258 8450 2583
Size: 195 5480 192 5933
Fragments arranged by size:
5933 5480 195 192
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2132 2151 2253 2264 2283 2514
Size: 399 334 8 19 102 11 19 231
Cuts at: 2514 2575 3226 3373 3451 3520 3667 3745 3814
Size: 61 651 147 78 69 147 78 69
Cuts at: 3814 3892 3964 4036 4108 4180 4252 4393 4967
Size: 78 72 72 72 72 72 141 574
Cuts at: 4967 5011 5031 5064 5136 5319 5477 5508 5664
Size: 44 20 33 72 183 158 31 156
Cuts at: 5664 5759 5768 5871 6320 6371 6399 6443 6510
Size: 95 9 103 449 51 28 44 67
Cuts at: 6510 6548 6709 6730 6768 6956 7061 7079 7091
Size: 38 161 21 38 188 105 18 12
Cuts at: 7091 7226 7748 7778 7848 7882 8041 8139 8184
Size: 135 522 30 70 34 159 98 45
Cuts at: 8184 8236 8281 8555 8659 8688 8925 9039 9119
Size: 52 45 274 104 29 237 114 80
Cuts at: 9119 9861 9946 11017 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 651 574 522 449 399
334 274 237 231 188 183 161 159
158 156 147 147 141 135 114 105
104 103 102 98 95 85 80 78
78 78 72 72 72 72 72 72
70 69 69 67 61 52 51 45
45 44 44 38 38 34 33 31
30 29 28 21 20 19 19 18
12 11 9 8
BspEI T'CCGG_A
Cuts at: 8565 11463 8565
Size: 2898 8902
BspHI T'CATG_A
Cuts at: 6502 9689 11419 6502
Size: 3187 1730 6883
Fragments arranged by size:
6883 3187 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2169 2185 5194 5838 6055 7527 8365 8602
Size: 1325 16 3009 644 217 1472 838 237
Cuts at: 8602 9048 9155 844
Size: 446 107 3489
Fragments arranged by size:
3489 3009 1472 1325 838 644 446 237
217 107 16
BsrBI CCG'CTC
Cuts at: 1394 1823 2707 4659 5803 7228 7933 7960 8407
Size: 429 884 1952 1144 1425 705 27 447
Cuts at: 8407 8833 9013 9687 1394
Size: 426 180 674 3507
Fragments arranged by size:
3507 1952 1425 1144 884 705 674 447
429 426 180 27
BsrDI GCAATG_nn'
Cuts at: 65 1248 8787 8815 10295 10469 65
Size: 1183 7539 28 1480 174 1396
Fragments arranged by size:
7539 1480 1396 1183 174 28
BsrFI r'CCGG_y
Cuts at: 1700 1810 2636 2712 7269 8064 10444 10631 1700
Size: 110 826 76 4557 795 2380 187 2869
Fragments arranged by size:
4557 2869 2380 826 795 187 110 76
BsrGI T'GTAC_A
Cuts at: 96 4547 96
Size: 4451 7349
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11800
BssSI C'ACGA_G
Cuts at: 3276 3345 3423 3492 3570 3639 3717 3786 3864
Size: 69 78 69 78 69 78 69 78
Cuts at: 3864 4365 5165 5312 5468 6541 8198 8402 9860
Size: 501 800 147 156 1073 1657 204 1458
Cuts at: 9860 10872 3276
Size: 1012 4204
Fragments arranged by size:
4204 1657 1458 1073 1012 800 501 204
156 147 78 78 78 78 69 69
69 69
BstAPI GCAn_nnn'nTGC
Cuts at: 794 8422 8692 8814 794
Size: 7628 270 122 3780
Fragments arranged by size:
7628 3780 270 122
BstBI TT'CG_AA
Cuts at: 10604 10604
Size: 11800
BstEII G'GTnAC_C
Cuts at: 1184 6067 7243 1184
Size: 4883 1176 5741
Fragments arranged by size:
5741 4883 1176
BstXI CCAn_nnnn'nTGG
Cuts at: 5848 6483 6613 6900 8297 5848
Size: 635 130 287 1397 9351
Fragments arranged by size:
9351 1397 635 287 130
BstYI r'GATC_y
Cuts at: 1972 5572 6118 6685 7575 8285 9130 9497 9882
Size: 3600 546 567 890 710 845 367 385
Cuts at: 9882 9899 11351 11437 11449 11795 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
3600 1977 1452 890 845 710 567 546
385 367 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2091 2184 2240 6153 7128 7313 7618 2091
Size: 93 56 3913 975 185 305 6273
Fragments arranged by size:
6273 3913 975 305 185 93 56
BtgI C'CryG_G
Cuts at: 513 1065 2004 2101 5454 8164 8466 9051 513
Size: 552 939 97 3353 2710 302 585 3262
Fragments arranged by size:
3353 3262 2710 939 585 552 302 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4798 487
Size: 683 56 3572 7489
Fragments arranged by size:
7489 3572 683 56
BtsI GCAGTG_nn'
Cuts at: 2523 2799 5885 6606 6938 7077 7427 7479 8142
Size: 276 3086 721 332 139 350 52 663
Cuts at: 8142 8912 9316 10110 10130 2523
Size: 770 404 794 20 4193
Fragments arranged by size:
4193 3086 794 770 721 663 404 350
332 276 139 52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11765
DraI TTT'AAA
Cuts at: 9104 9104
Size: 11800
DraIII CAC_nnn'GTG
Cuts at: 2753 3149 5708 6993 2753
Size: 396 2559 1285 7560
Fragments arranged by size:
7560 2559 1285 396
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 10807 809
Size: 507 9491 1802
Fragments arranged by size:
9491 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4739 4823 5823
Size: 54 1425 50 275 18 2909 84 1000
Cuts at: 5823 5911 6250 6819 6995 7608 7911 8194 8299
Size: 88 339 569 176 613 303 283 105
Cuts at: 8299 8749 9054 9247 10136 11765 8
Size: 450 305 193 889 1629 43
Fragments arranged by size:
2909 1629 1425 1000 889 613 569 450
339 305 303 283 275 193 176 105
88 84 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 9247 1537
Size: 275 7435 4090
Fragments arranged by size:
7435 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2283 3079 4774 4942 5089 5567 5939 6140
Size: 930 796 1695 168 147 478 372 201
Cuts at: 6140 8232 8502 9730 1353
Size: 2092 270 1228 3423
Fragments arranged by size:
3423 2092 1695 1228 930 796 478 372
270 201 168 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2413 6121 7270 7645 7826 10387
Size: 1064 138 824 3708 1149 375 181 2561
Cuts at: 10387 10761 10907 387
Size: 374 146 1280
Fragments arranged by size:
3708 2561 1280 1149 1064 824 375 374
181 146 138
EcoICRI GAG'CTC
Cuts at: 2122 2512 6508 6766 7846 8923 2122
Size: 390 3996 258 1080 1077 4999
Fragments arranged by size:
4999 3996 1080 1077 390 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2118 2369 2395 2723 2857 2887 2917
Size: 393 486 251 26 328 134 30 30
Cuts at: 2917 3232 3379 3526 3673 3820 5306 6155 6263
Size: 315 147 147 147 147 1486 849 108
Cuts at: 6263 6364 6700 7067 7398 7656 7772 7949 8123
Size: 101 336 367 331 258 116 177 174
Cuts at: 8123 8228 8261 8528 8618 8666 8867 8909 9047
Size: 105 33 267 90 48 201 42 138
Cuts at: 9047 9050 9863 10460 11247 11515 1239
Size: 3 813 597 787 268 1524
Fragments arranged by size:
1524 1486 849 813 787 597 486 393
367 336 331 328 315 268 267 258
251 201 177 174 147 147 147 147
138 134 116 108 105 101 90 48
42 33 30 30 26 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2601 8048 8426 8450 8759 8894 9095 2601
Size: 5447 378 24 309 135 201 5306
Fragments arranged by size:
5447 5306 378 309 201 135 24
EcoO109I rG'GnC_Cy
Cuts at: 2188 2249 2260 2294 2353 2389 2494 2592 2877
Size: 61 11 34 59 36 105 98 285
Cuts at: 2877 2907 2937 2967 2997 4341 5389 5416 5867
Size: 30 30 30 30 1344 1048 27 451
Cuts at: 5867 5868 5970 6177 6207 6309 6316 6317 6368
Size: 1 102 207 30 102 7 1 51
Cuts at: 6368 6566 6754 6793 6845 7058 7087 7088 7309
Size: 198 188 39 52 213 29 1 221
Cuts at: 7309 7744 7745 7936 7992 8029 8180 8181 8551
Size: 435 1 191 56 37 151 1 370
Cuts at: 8551 2188
Size: 5437
Fragments arranged by size:
5437 1344 1048 451 435 370 285 221
213 207 198 191 188 151 105 102
102 98 61 59 56 52 51 39
37 36 34 30 30 30 30 30
29 27 11 7 1 1 1 1
1
EcoRI G'AATT_C
Cuts at: 3205 4684 9109 9487 3205
Size: 1479 4425 378 5518
Fragments arranged by size:
5518 4425 1479 378
FspI TGC'GCA
Cuts at: 9083 10306 9083
Size: 1223 10577
HaeII r_GCGC'y
Cuts at: 1678 2202 5774 5932 7951 8176 8422 8950 10947
Size: 524 3572 158 2019 225 246 528 1997
Cuts at: 10947 1678
Size: 2531
Fragments arranged by size:
3572 2531 2019 1997 528 524 246 225
158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2381 2393 2413 2425 4333
Size: 32 135 32 809 12 20 12 1908
Cuts at: 4333 4365 4741 4773 4840 4872 5504 5536 5981
Size: 32 376 32 67 32 632 32 445
Cuts at: 5981 6013 6705 6737 6863 6895 7018 7050 7630
Size: 32 692 32 126 32 123 32 580
Cuts at: 7630 7648 7662 7680 8345 8377 9059 9091 10441
Size: 18 14 18 665 32 682 32 1350
Cuts at: 10441 10473 10515 10547 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1908 1350 809 692 682 665 632
580 445 376 135 126 123 67 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 20 18
18 14 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2718 9144 9390 669
Size: 988 281 780 6426 246 3079
Fragments arranged by size:
6426 3079 988 780 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 11800
HpaI GTT'AAC
Cuts at: 9390 9390
Size: 11800
KpnI G_GTAC'C
Cuts at: 5353 9125 5353
Size: 3772 8028
MfeI C'AATT_G
Cuts at: 9399 9399
Size: 11800
MluI A'CGCG_T
Cuts at: 11676 11676
Size: 11800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4030 4102 4318 7138 7691 10914 11098 1305
Size: 2725 72 216 2820 553 3223 184 2007
Fragments arranged by size:
3223 2820 2725 2007 553 216 184 72
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6252 6821 6997 7913 8301
Size: 54 1425 343 4420 569 176 916 388
Cuts at: 8301 10
Size: 3509
Fragments arranged by size:
4420 3509 1425 916 569 388 343 176
54
MslI CAynn'nnrTG
Cuts at: 518 1166 4612 5067 5208 5338 5492 5511 5667
Size: 648 3446 455 141 130 154 19 156
Cuts at: 5667 6611 6810 9758 10117 10276 518
Size: 944 199 2948 359 159 2042
Fragments arranged by size:
3446 2948 2042 944 648 455 359 199
159 156 154 141 130 19
MspA1I CmG'CkG
Cuts at: 1606 2070 2303 2379 2502 3127 3229 3307 3376
Size: 464 233 76 123 625 102 78 69
Cuts at: 3376 3454 3523 3601 3670 3748 3817 3895 4255
Size: 78 69 78 69 78 69 78 360
Cuts at: 4255 4396 4555 4997 5889 5966 6184 6800 6964
Size: 141 159 442 892 77 218 616 164
Cuts at: 6964 7768 7855 7876 8166 8239 8395 8700 8776
Size: 804 87 21 290 73 156 305 76
Cuts at: 8776 9028 9216 9893 11041 11286 1606
Size: 252 188 677 1148 245 2120
Fragments arranged by size:
2120 1148 892 804 677 625 616 464
442 360 305 290 252 245 233 218
188 164 159 156 141 123 102 87
78 78 78 78 78 77 76 76
73 69 69 69 69 21
NcoI C'CATG_G
Cuts at: 513 1065 2004 2101 5454 9051 513
Size: 552 939 97 3353 3597 3262
Fragments arranged by size:
3597 3353 3262 939 552 97
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11800
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11800
NotI GC'GGCC_GC
Cuts at: 9247 9247
Size: 11800
NspI r_CATG'y
Cuts at: 1132 1229 8337 9158 10703 1132
Size: 97 7108 821 1545 2229
Fragments arranged by size:
7108 2229 1545 821 97
PasI CC'CwG_GG
Cuts at: 1348 2994 4719 4803 5424 6313 6371 7063 1348
Size: 1646 1725 84 621 889 58 692 6085
Fragments arranged by size:
6085 1725 1646 889 692 621 84 58
PciI A'CATG_T
Cuts at: 10699 10699
Size: 11800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2224 2753 4668 6923 6993 1108
Size: 1116 529 1915 2255 70 5915
Fragments arranged by size:
5915 2255 1915 1116 529 70
PfoI T'CCnGG_A
Cuts at: 5412 5568 8913 9063 5412
Size: 156 3345 150 8149
Fragments arranged by size:
8149 3345 156 150
PmeI GTTT'AAAC
Cuts at: 9104 9104
Size: 11800
PmlI CAC'GTG
Cuts at: 2822 2822
Size: 11800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3244 3276 3391 3423 3538 3570 3685 3717 3832
Size: 32 115 32 115 32 115 32 115
Cuts at: 3832 3864 7094 7126 9869 9901 11382 11414 3244
Size: 32 3230 32 2743 32 1481 32 3630
Fragments arranged by size:
3630 3230 2743 1481 115 115 115 115
32 32 32 32 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2188 2294 2389 2592 4341 5416 6177 6207 6309
Size: 106 95 203 1749 1075 761 30 102
Cuts at: 6309 6793 6845 7309 7936 7992 2188
Size: 484 52 464 627 56 5996
Fragments arranged by size:
5996 1749 1075 761 627 484 464 203
106 102 95 56 52 30
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 11800
PsiI TTA'TAA
Cuts at: 9370 9370
Size: 11800
PspOMI G'GGCC_C
Cuts at: 2249 2260 5867 6316 6367 6726 7057 7087 7744
Size: 11 3607 449 51 359 331 30 657
Cuts at: 7744 7878 8180 8232 8551 2249
Size: 134 302 52 319 5498
Fragments arranged by size:
5498 3607 657 449 359 331 319 302
134 52 51 30 11
PspXI vC'TCGA_Gb
Cuts at: 5076 5076
Size: 11800
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3061 3093 4303 4335 3061
Size: 32 1210 32 10526
Fragments arranged by size:
10526 1210 32 32
PstI C_TGCA'G
Cuts at: 830 2617 6774 6945 7110 8394 8418 8427 8613
Size: 1787 4157 171 165 1284 24 9 186
Cuts at: 8613 9152 830
Size: 539 3478
Fragments arranged by size:
4157 3478 1787 1284 539 186 171 165
24 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11800
PvuII CAG'CTG
Cuts at: 2303 2379 2502 6800 6964 8239 8776 9028 2303
Size: 76 123 4298 164 1275 537 252 5075
Fragments arranged by size:
5075 4298 1275 537 252 164 123 76
SacI G_AGCT'C
Cuts at: 2124 2514 6510 6768 7848 8925 2124
Size: 390 3996 258 1080 1077 4999
Fragments arranged by size:
4999 3996 1080 1077 390 258
SacII CC_GC'GG
Cuts at: 8167 8167
Size: 11800
SalI G'TCGA_C
Cuts at: 1936 9142 1936
Size: 7206 4594
SanDI GG'GwC_CC
Cuts at: 2294 6177 6309 6845 2294
Size: 3883 132 536 7249
Fragments arranged by size:
7249 3883 536 132
SapI GCTCTTCn'nnn_
Cuts at: 8232 8502 8232
Size: 270 11530
SbfI CC_TGCA'GG
Cuts at: 6774 9152 6774
Size: 2378 9422
ScaI AGT'ACT
Cuts at: 8630 10048 11760 8630
Size: 1418 1712 8670
Fragments arranged by size:
8670 1712 1418
SexAI A'CCwGG_T
Cuts at: 2588 2588
Size: 11800
SfcI C'TryA_G
Cuts at: 826 1045 2613 6770 6941 7106 7729 8390 8414
Size: 219 1568 4157 171 165 623 661 24
Cuts at: 8414 8423 8609 9148 10283 10964 11155 826
Size: 9 186 539 1135 681 191 1471
Fragments arranged by size:
4157 1568 1471 1135 681 661 623 539
219 191 186 171 165 24 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 5978 6258 8559 5978
Size: 280 2301 9219
Fragments arranged by size:
9219 2301 280
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11800
SgrAI Cr'CCGG_yG
Cuts at: 1700 2636 1700
Size: 936 10864
SmaI CCC'GGG
Cuts at: 1872 4928 5219 5366 5762 5779 7264 8313 9127
Size: 3056 291 147 396 17 1485 1049 814
Cuts at: 9127 1872
Size: 4545
Fragments arranged by size:
4545 3056 1485 1049 814 396 291 147
17
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2330 5076 7229 9904 10805 11067
Size: 197 926 387 2746 2153 2675 901 262
Cuts at: 11067 820
Size: 1553
Fragments arranged by size:
2746 2675 2153 1553 926 901 387 262
197
SnaBI TAC'GTA
Cuts at: 493 9591 493
Size: 9098 2702
SpeI A'CTAG_T
Cuts at: 152 5585 152
Size: 5433 6367
SphI G_CATG'C
Cuts at: 1132 9158 1132
Size: 8026 3774
SspI AAT'ATT
Cuts at: 5 52 7466 9724 5
Size: 47 7414 2258 2081
Fragments arranged by size:
7414 2258 2081 47
StuI AGG'CCT
Cuts at: 1517 2238 2536 4843 6363 6482 7044 7829 8498
Size: 721 298 2307 1520 119 562 785 669
Cuts at: 8498 1517
Size: 4819
Fragments arranged by size:
4819 2307 1520 785 721 669 562 298
119
StyI C'CwwG_G
Cuts at: 513 1065 2004 2101 2963 4831 5454 6013 7584
Size: 552 939 97 862 1868 623 559 1571
Cuts at: 7584 7830 8257 9051 9239 513
Size: 246 427 794 188 3074
Fragments arranged by size:
3074 1868 1571 939 862 794 623 559
552 427 246 188 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2873 2903 2933 2963 2993 3065 3947
Size: 1255 1293 30 30 30 30 72 882
Cuts at: 3947 4163 4448 4637 4922 5258 5715 5719 5854
Size: 216 285 189 285 336 457 4 135
Cuts at: 5854 6619 7004 7751 8024 9827 9844 9997 10182
Size: 765 385 747 273 1803 17 153 185
Cuts at: 10182 10627 325
Size: 445 1498
Fragments arranged by size:
1803 1498 1293 1255 882 765 747 457
445 385 336 285 285 273 216 189
185 153 135 72 30 30 30 30
17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3116 4547 4911
Size: 274 80 33 51 1443 1139 1431 364
Cuts at: 4911 5811 6453 8628 10046 11758 96
Size: 900 642 2175 1418 1712 138
Fragments arranged by size:
2175 1712 1443 1431 1418 1139 900 642
364 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2015 2047 2811 2843 3133 3135 3165 3167 8423
Size: 32 764 32 290 2 30 2 5256
Cuts at: 8423 8455 8526 8558 8891 8923 11760 11792 2015
Size: 32 71 32 333 32 2837 32 2023
Fragments arranged by size:
5256 2837 2023 764 333 290 71 32
32 32 32 32 32 30 2 2
Tth111I GACn'n_nGTC
Cuts at: 1316 9004 1316
Size: 7688 4112
XbaI T'CTAG_A
Cuts at: 9136 9136
Size: 11800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2596 4895 5576 5794 8744 2596
Size: 2299 681 218 2950 5652
Fragments arranged by size:
5652 2950 2299 681 218
XhoI C'TCGA_G
Cuts at: 1943 5076 1943
Size: 3133 8667
XmaI C'CCGG_G
Cuts at: 1870 4926 5217 5364 5760 5777 7262 8311 9125
Size: 3056 291 147 396 17 1485 1049 814
Cuts at: 9125 1870
Size: 4545
Fragments arranged by size:
4545 3056 1485 1049 814 396 291 147
17
XmnI GAAnn'nnTTC
Cuts at: 3073 4768 9929 3073
Size: 1695 5161 4944
Fragments arranged by size:
5161 4944 1695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8974 9608 276
Size: 53 83 186 936 7440 634 2468
Fragments arranged by size:
7440 2468 936 634 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI BbeI BfrBI BstZ17I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI RsrII
SfoI SrfI SwaI