Restriction map for
pFN21AA1649
(Circular) (Six-base) MAPSORT of: pFN21AA1649.seq Check: 1190 from: 1 to: 5968
pFN21AA1649 5968 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3778 278
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3311 1937
Size: 1374 4594
Acc65I G'GTAC_C
Cuts at: 3289 3289
Size: 5968
AclI AA'CG_TT
Cuts at: 1563 4095 4468 1563
Size: 2532 373 3063
Fragments arranged by size:
3063 2532 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2453 3221 4031 5415 5683 1632
Size: 821 768 810 1384 268 1917
Fragments arranged by size:
1917 1384 821 810 768 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5771
AflIII A'CryG_T
Cuts at: 1203 2760 4867 5844 1203
Size: 1557 2107 977 1327
Fragments arranged by size:
2107 1557 1327 977
AgeI A'CCGG_T
Cuts at: 3012 4799 3012
Size: 1787 4181
AhdI GACnn_n'nnGTC
Cuts at: 4697 4697
Size: 5968
AleI CACnn'nnGTG
Cuts at: 1166 2690 1166
Size: 1524 4444
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2265 1184
Size: 1081 4887
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3140 3172 3140
Size: 32 5936
AlwNI CAG_nnn'CTG
Cuts at: 2720 2813 3711 5283 2720
Size: 93 898 1572 3405
Fragments arranged by size:
3405 1572 898 93
ApaI G_GGCC'C
Cuts at: 2920 2920
Size: 5968
ApaLI G'TGCA_C
Cuts at: 4025 5181 4025
Size: 1156 4812
ApoI r'AATT_y
Cuts at: 1493 2542 3251 3277 3503 3655 5764 1493
Size: 1049 709 26 226 152 2109 1697
Fragments arranged by size:
2109 1697 1049 709 226 152 26
AseI AT'TA_AT
Cuts at: 160 1032 4522 160
Size: 872 3490 1606
Fragments arranged by size:
3490 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
AvaI C'yCGr_G
Cuts at: 1870 1943 2100 2226 2355 2871 3293 1870
Size: 73 157 126 129 516 422 4545
Fragments arranged by size:
4545 516 422 157 129 126 73
BamHI G'GATC_C
Cuts at: 2109 3298 3665 2109
Size: 1189 367 4412
Fragments arranged by size:
4412 1189 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2323 2476 2840 3289 4744
Size: 325 217 627 536 153 364 449 1455
Cuts at: 4744 618
Size: 1842
Fragments arranged by size:
1842 1455 627 536 449 364 325 217
153
BanII G_rGCy'C
Cuts at: 1391 2920 2932 3287 1391
Size: 1529 12 355 4072
Fragments arranged by size:
4072 1529 355 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4997
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4159 4193 1903
Size: 34 2222 34 3678
Fragments arranged by size:
3678 2222 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3860 5070 3860
Size: 1210 4758
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5968
BfrBI ATG'CAT
Cuts at: 2708 2708
Size: 5968
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4579 136
Size: 107 122 71 631 605 2907 1525
Fragments arranged by size:
2907 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5963 5963
Size: 5968
BlpI GC'TnA_GC
Cuts at: 2645 2814 3385 2645
Size: 169 571 5228
Fragments arranged by size:
5228 571 169
Bme1580I G_kGCm'C
Cuts at: 1790 2328 2920 4029 5185 1790
Size: 538 592 1109 1156 2573
Fragments arranged by size:
2573 1156 1109 592 538
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5968
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2219 3699 4657
Size: 416 53 83 399 24 802 1480 958
Cuts at: 4657 442
Size: 1753
Fragments arranged by size:
1753 1480 958 802 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4628 1239
Size: 3389 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2906 4093 4958 5256 2906
Size: 1187 865 298 3618
Fragments arranged by size:
3618 1187 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4631 882
Size: 617 3132 2219
Fragments arranged by size:
3132 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3759 493
Size: 711 2555 2702
Fragments arranged by size:
2702 2555 711
BsaBI GATnn'nnATC
Cuts at: 5962 5962
Size: 5968
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3775 4157 4792 275
Size: 53 83 186 936 2242 382 635 1451
Fragments arranged by size:
2242 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3012 4401 4799 5073 5220 5631 3012
Size: 1389 398 274 147 411 3349
Fragments arranged by size:
3349 1389 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2948 2978 3140 3170 1178
Size: 30 165 30 1545 30 162 30 3976
Fragments arranged by size:
3976 1545 165 162 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2392 2897 3148 3151 1186
Size: 1206 505 251 3 4003
Fragments arranged by size:
4003 1206 505 251 3
BseYI C'CCAG_C
Cuts at: 2639 5171 5667 2639
Size: 2532 496 2940
Fragments arranged by size:
2940 2532 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2297 2849 1163
Size: 573 561 552 4282
Fragments arranged by size:
4282 573 561 552
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3418 4179 5207 1540
Size: 275 186 1417 761 1028 2301
Fragments arranged by size:
2301 1417 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2932 4029 4114 5185 2932
Size: 1097 85 1071 3715
Fragments arranged by size:
3715 1097 1071 85
BsmI GAATG_Cn'
Cuts at: 3479 3572 3479
Size: 93 5875
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2328 2920 2932 3287 4029 4114 5185
Size: 399 538 592 12 355 742 85 1071
Cuts at: 5185 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 592 538 399 355 85
12
BspEI T'CCGG_A
Cuts at: 5631 5631
Size: 5968
BspHI T'CATG_A
Cuts at: 3857 5587 3857
Size: 1730 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3323 844
Size: 2479 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3855 1394
Size: 429 2032 3507
Fragments arranged by size:
3507 2032 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4463 4637 65
Size: 1183 3215 174 1396
Fragments arranged by size:
3215 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3012 4612 4799 1700
Size: 110 1202 1600 187 2869
Fragments arranged by size:
2869 1600 1202 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5968
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5968
BssSI C'ACGA_G
Cuts at: 4028 5040 4028
Size: 1012 4956
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5968
BstBI TT'CG_AA
Cuts at: 2260 2740 4772 2260
Size: 480 2032 3456
Fragments arranged by size:
3456 2032 480
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5968
BstXI CCAn_nnnn'nTGG
Cuts at: 2639 2639
Size: 5968
BstYI r'GATC_y
Cuts at: 1972 2109 3298 3665 4050 4067 5519 5605 5617
Size: 137 1189 367 385 17 1452 86 12
Cuts at: 5617 5963 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1189 385 367 346 137 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2614 3220 2614
Size: 606 5362
BtgI C'CryG_G
Cuts at: 513 1065 2004 2319 2472 513
Size: 552 939 315 153 4009
Fragments arranged by size:
4009 939 552 315 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5229
Fragments arranged by size:
5229 683 56
BtsI GCAGTG_nn'
Cuts at: 3484 4278 4298 3484
Size: 794 20 5154
Fragments arranged by size:
5154 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5968
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5933
DraI TTT'AAA
Cuts at: 3272 3272
Size: 5968
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2761 4975 809
Size: 507 1445 2214 1802
Fragments arranged by size:
2214 1802 1445 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3415 4304 5933
Size: 54 1425 50 275 18 1585 889 1629
Cuts at: 5933 8
Size: 43
Fragments arranged by size:
1629 1585 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3415 1537
Size: 275 1603 4090
Fragments arranged by size:
4090 1603 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2369 3215 3898 1353
Size: 1016 846 683 3423
Fragments arranged by size:
3423 1016 846 683
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4555 4929 5075 387
Size: 1064 138 435 2531 374 146 1280
Fragments arranged by size:
2531 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2930 2930
Size: 5968
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2453 3221 4031 4628 5415 5683 1239
Size: 393 821 768 810 597 787 268 1524
Fragments arranged by size:
1524 821 810 787 768 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2674 2916 2917 3225 2674
Size: 242 1 308 5417
Fragments arranged by size:
5417 308 242 1
EcoRI G'AATT_C
Cuts at: 3251 3277 3655 3251
Size: 26 378 5564
Fragments arranged by size:
5564 378 26
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2081 2386 3226 2081
Size: 305 840 4823
Fragments arranged by size:
4823 840 305
FspI TGC'GCA
Cuts at: 4474 4474
Size: 5968
HaeII r_GCGC'y
Cuts at: 1678 5115 1678
Size: 3437 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2094 2126 2247 2279 2413
Size: 32 135 32 522 32 121 32 134
Cuts at: 2413 2445 3095 3127 4609 4641 4683 4715 1373
Size: 32 650 32 1482 32 42 32 2626
Fragments arranged by size:
2626 1482 650 522 135 134 121 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2136 3312 3558 669
Size: 988 281 198 1176 246 3079
Fragments arranged by size:
3079 1176 988 281 246 198
HindIII A'AGCT_T
Cuts at: 748 2234 2657 3263 748
Size: 1486 423 606 3453
Fragments arranged by size:
3453 1486 606 423
HpaI GTT'AAC
Cuts at: 3558 3558
Size: 5968
KpnI G_GTAC'C
Cuts at: 3293 3293
Size: 5968
MfeI C'AATT_G
Cuts at: 3567 3567
Size: 5968
MluI A'CGCG_T
Cuts at: 5844 5844
Size: 5968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2065 3189 5082 5266 1305
Size: 760 1124 1893 184 2007
Fragments arranged by size:
2007 1893 1124 760 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4146
Fragments arranged by size:
4146 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2690 2846 3054 3926 4285 4444 518
Size: 648 1524 156 208 872 359 159 2042
Fragments arranged by size:
2042 1524 872 648 359 208 159 156
MspA1I CmG'CkG
Cuts at: 1606 2810 3384 4061 5209 5454 1606
Size: 1204 574 677 1148 245 2120
Fragments arranged by size:
2120 1204 1148 677 574 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2472 513
Size: 552 939 468 4009
Fragments arranged by size:
4009 939 552 468
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5968
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5968
NotI GC'GGCC_GC
Cuts at: 3415 3415
Size: 5968
NsiI A_TGCA'T
Cuts at: 2710 2710
Size: 5968
NspI r_CATG'y
Cuts at: 1132 1229 2764 3326 4871 1132
Size: 97 1535 562 1545 2229
Fragments arranged by size:
2229 1545 1535 562 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5968
PciI A'CATG_T
Cuts at: 2760 4867 2760
Size: 2107 3861
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2411 2635 1108
Size: 1303 224 4441
Fragments arranged by size:
4441 1303 224
PfoI T'CCnGG_A
Cuts at: 2765 2765
Size: 5968
PmeI GTTT'AAAC
Cuts at: 3272 3272
Size: 5968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3140 3172 4037 4069 5550 5582 3140
Size: 32 865 32 1481 32 3526
Fragments arranged by size:
3526 1481 865 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5968
PsiI TTA'TAA
Cuts at: 3538 3538
Size: 5968
PspOMI G'GGCC_C
Cuts at: 2916 2916
Size: 5968
PspXI vC'TCGA_Gb
Cuts at: 2871 2871
Size: 5968
PstI C_TGCA'G
Cuts at: 830 3320 830
Size: 2490 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5968
PvuII CAG'CTG
Cuts at: 2810 2810
Size: 5968
SacI G_AGCT'C
Cuts at: 2932 2932
Size: 5968
SalI G'TCGA_C
Cuts at: 1936 3310 1936
Size: 1374 4594
SbfI CC_TGCA'GG
Cuts at: 3320 3320
Size: 5968
ScaI AGT'ACT
Cuts at: 4216 5928 4216
Size: 1712 4256
SfcI C'TryA_G
Cuts at: 826 1045 3316 4451 5132 5323 826
Size: 219 2271 1135 681 191 1471
Fragments arranged by size:
2271 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5968
SmaI CCC'GGG
Cuts at: 1872 2228 2357 3295 1872
Size: 356 129 938 4545
Fragments arranged by size:
4545 938 356 129
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2100 2871 2921 4072 4973 5235
Size: 197 926 157 771 50 1151 901 262
Cuts at: 5235 820
Size: 1553
Fragments arranged by size:
1553 1151 926 901 771 262 197 157
50
SnaBI TAC'GTA
Cuts at: 493 3759 493
Size: 3266 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5968
SphI G_CATG'C
Cuts at: 1132 3326 1132
Size: 2194 3774
SspI AAT'ATT
Cuts at: 5 52 3892 5
Size: 47 3840 2081
Fragments arranged by size:
3840 2081 47
StuI AGG'CCT
Cuts at: 1517 2121 1517
Size: 604 5364
StyI C'CwwG_G
Cuts at: 513 1065 2004 2472 2670 3407 513
Size: 552 939 468 198 737 3074
Fragments arranged by size:
3074 939 737 552 468 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2422 3995 4012 4165 4350 4795 325
Size: 1255 842 1573 17 153 185 445 1498
Fragments arranged by size:
1573 1498 1255 842 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3003 4214 5926
Size: 274 80 33 51 1443 1026 1211 1712
Cuts at: 5926 96
Size: 138
Fragments arranged by size:
1712 1443 1211 1026 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5928 5960 5928
Size: 32 5936
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5968
XbaI T'CTAG_A
Cuts at: 3304 3304
Size: 5968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2636 2636
Size: 5968
XhoI C'TCGA_G
Cuts at: 1943 2100 2871 1943
Size: 157 771 5040
Fragments arranged by size:
5040 771 157
XmaI C'CCGG_G
Cuts at: 1870 2226 2355 3293 1870
Size: 356 129 938 4545
Fragments arranged by size:
4545 938 356 129
XmnI GAAnn'nnTTC
Cuts at: 4097 4097
Size: 5968
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3776 276
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI